GB2572877A - Polynucleotide libraries having controlled stoichiometry and synthesis thereof - Google Patents
Polynucleotide libraries having controlled stoichiometry and synthesis thereof Download PDFInfo
- Publication number
- GB2572877A GB2572877A GB1908719.6A GB201908719A GB2572877A GB 2572877 A GB2572877 A GB 2572877A GB 201908719 A GB201908719 A GB 201908719A GB 2572877 A GB2572877 A GB 2572877A
- Authority
- GB
- United Kingdom
- Prior art keywords
- synthesis
- libraries
- polynucleotide libraries
- polynucleotides
- controlled stoichiometry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 4
- 108091033319 polynucleotide Proteins 0.000 title abstract 4
- 239000002157 polynucleotide Substances 0.000 title abstract 4
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000007481 next generation sequencing Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662424302P | 2016-11-18 | 2016-11-18 | |
| US201762548307P | 2017-08-21 | 2017-08-21 | |
| US201762558666P | 2017-09-14 | 2017-09-14 | |
| PCT/US2017/062391 WO2018094263A1 (en) | 2016-11-18 | 2017-11-17 | Polynucleotide libraries having controlled stoichiometry and synthesis thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB201908719D0 GB201908719D0 (en) | 2019-07-31 |
| GB2572877A true GB2572877A (en) | 2019-10-16 |
Family
ID=62144787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1908719.6A Withdrawn GB2572877A (en) | 2016-11-18 | 2017-11-17 | Polynucleotide libraries having controlled stoichiometry and synthesis thereof |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20180142289A1 (enExample) |
| EP (1) | EP3541973B1 (enExample) |
| JP (3) | JP2020504709A (enExample) |
| KR (1) | KR102569164B1 (enExample) |
| CN (1) | CN110382752A (enExample) |
| AU (2) | AU2017363204B2 (enExample) |
| CA (1) | CA3044057A1 (enExample) |
| GB (1) | GB2572877A (enExample) |
| IL (2) | IL266681B2 (enExample) |
| WO (1) | WO2018094263A1 (enExample) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102160389B1 (ko) | 2013-08-05 | 2020-09-28 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
| WO2017053450A1 (en) | 2015-09-22 | 2017-03-30 | Twist Bioscience Corporation | Flexible substrates for nucleic acid synthesis |
| EP3384077A4 (en) | 2015-12-01 | 2019-05-08 | Twist Bioscience Corporation | FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF |
| EP3452614B1 (en) | 2016-05-02 | 2023-06-28 | Becton, Dickinson and Company | Accurate molecular barcoding |
| WO2018038772A1 (en) | 2016-08-22 | 2018-03-01 | Twist Bioscience Corporation | De novo synthesized nucleic acid libraries |
| EP4664304A3 (en) | 2016-09-21 | 2026-01-21 | Atlas Data Storage, Inc. | Nucleic acid based data storage |
| AU2017363204B2 (en) * | 2016-11-18 | 2022-12-08 | Twist Bioscience Corporation | Polynucleotide libraries having controlled stoichiometry and synthesis thereof |
| GB2573069A (en) | 2016-12-16 | 2019-10-23 | Twist Bioscience Corp | Variant libraries of the immunological synapse and synthesis thereof |
| WO2018156792A1 (en) | 2017-02-22 | 2018-08-30 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CA3056388A1 (en) | 2017-03-15 | 2018-09-20 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| CN111566125A (zh) | 2017-09-11 | 2020-08-21 | 特韦斯特生物科学公司 | Gpcr结合蛋白及其合成 |
| CN111565834B (zh) | 2017-10-20 | 2022-08-26 | 特韦斯特生物科学公司 | 用于多核苷酸合成的加热的纳米孔 |
| IL312616B2 (en) | 2018-01-04 | 2025-08-01 | Twist Bioscience Corp | Dna-based digital information storage |
| EP3814497A4 (en) | 2018-05-18 | 2022-03-02 | Twist Bioscience Corporation | POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION |
| EP3902816A4 (en) | 2018-12-26 | 2022-09-14 | Twist Bioscience Corporation | HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS |
| WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| KR20210144698A (ko) | 2019-02-26 | 2021-11-30 | 트위스트 바이오사이언스 코포레이션 | 항체 최적화를 위한 변이 핵산 라이브러리 |
| CA3131689A1 (en) | 2019-02-26 | 2020-09-03 | Twist Bioscience Corporation | Variant nucleic acid libraries for glp1 receptor |
| EP3987019A4 (en) | 2019-06-21 | 2023-04-19 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| CN115023440B (zh) | 2019-09-23 | 2025-09-12 | 特韦斯特生物科学公司 | 结合CD3ε的抗体 |
| EP4055159A4 (en) | 2019-11-06 | 2024-04-17 | Adaptive Biotechnologies Corporation | SYNTHETIC STRANDS FOR NUCLEIC ACID SEQUENCING AND RELATED METHODS AND SYSTEMS |
| CN110853708B (zh) * | 2019-11-13 | 2022-03-08 | 上海仁东医学检验所有限公司 | 用于hla分型的核酸捕获探针的设计方法 |
| AU2020400030B2 (en) | 2019-12-09 | 2026-05-21 | Twist Bioscience Corporation | Variant nucleic acid libraries for adenosine receptors |
| BR112022021780A2 (pt) | 2020-04-27 | 2023-03-07 | Twist Bioscience Corp | Bibliotecas de ácido nucléico variantes para coronavírus |
| WO2021231324A1 (en) * | 2020-05-11 | 2021-11-18 | Personal Genome Diagnostics Inc. | Selective capture of target dna sequences |
| WO2021242668A1 (en) * | 2020-05-28 | 2021-12-02 | Illumina, Inc. | Comparing copies of polynucleotides with different features |
| CA3190667A1 (en) | 2020-08-26 | 2022-03-03 | Aaron Sato | Methods and compositions relating to glp1r variants |
| US12534494B2 (en) | 2020-08-28 | 2026-01-27 | Twist Bioscience Corporation | Devices and methods for synthesis |
| EP4229210A4 (en) | 2020-10-19 | 2025-01-08 | Twist Bioscience Corporation | METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES |
| WO2022159620A1 (en) | 2021-01-21 | 2022-07-28 | Twist Bioscience Corporation | Methods and compositions relating to adenosine receptors |
| BE1029144B1 (fr) * | 2021-02-25 | 2022-09-20 | Oncodna | Méthode de caracterisation d'une tumeur à l'aide d'un séquençage ciblé |
| US12258406B2 (en) | 2021-03-24 | 2025-03-25 | Twist Bioscience Corporation | Antibodies that bind CD3 Epsilon |
| WO2022235584A1 (en) | 2021-05-03 | 2022-11-10 | Twist Bioscience Corporation | Variant nucleic acid libraries for glycans |
| WO2022271884A2 (en) | 2021-06-22 | 2022-12-29 | Twist Bioscience Corporation | Methods and compositions relating to covid antibody epitopes |
| WO2023023285A2 (en) | 2021-08-19 | 2023-02-23 | Twist Bioscience Corporation | Methods and compositions relating to covalently closed nucleic acids |
| US12697597B2 (en) | 2021-11-01 | 2026-08-04 | Twist Bioscience Corporation | Methods for polynucleotide synthesis |
| EP4433085A2 (en) | 2021-11-18 | 2024-09-25 | Twist Bioscience Corporation | Dickkopf-1 variant antibodies and methods of use |
| US12325739B2 (en) | 2022-01-03 | 2025-06-10 | Twist Bioscience Corporation | Bispecific SARS-CoV-2 antibodies and methods of use |
| EP4490318A4 (en) | 2022-03-07 | 2026-06-10 | Twist Bioscience Corp | METHYLATION-MEDIATED ADAPTER ELIMINATION ON NUCLEIC ACID SEQUENCES |
| CN116417071B (zh) * | 2023-04-07 | 2026-04-03 | 祥符实验室 | 一种dna数据存储的选择性访问方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150056609A1 (en) * | 2012-05-09 | 2015-02-26 | Longhorn Vaccines And Diagnostics, Llc | Next Generation Genomic Sequencing Methods |
| US20160032396A1 (en) * | 2013-03-15 | 2016-02-04 | The Board Of Trustees Of The Leland Stanford Junior University | Identification and Use of Circulating Nucleic Acid Tumor Markers |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5474796A (en) | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US7655791B2 (en) * | 2001-11-13 | 2010-02-02 | Rubicon Genomics, Inc. | DNA amplification and sequencing using DNA molecules generated by random fragmentation |
| WO2010030683A1 (en) * | 2008-09-09 | 2010-03-18 | Rosetta Inpharmatics Llc | Methods of generating gene specific libraries |
| US8828688B2 (en) * | 2010-05-27 | 2014-09-09 | Affymetrix, Inc. | Multiplex amplification methods |
| JP6017458B2 (ja) * | 2011-02-02 | 2016-11-02 | ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション | 大量並列連続性マッピング |
| CA2871505C (en) * | 2012-04-24 | 2021-10-12 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| EP2880182B1 (en) * | 2012-08-02 | 2017-07-26 | Qiagen GmbH | Recombinase mediated targeted dna enrichment for next generation sequencing |
| KR102160389B1 (ko) * | 2013-08-05 | 2020-09-28 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| GB2534067B (en) * | 2013-08-30 | 2021-07-21 | Personalis Inc | Methods and systems for genomic analysis |
| CA2929108A1 (en) * | 2013-10-29 | 2015-05-07 | Longhorn Vaccines And Diagnostics, Llc | Next generation genomic sequencing methods |
| AU2017363204B2 (en) * | 2016-11-18 | 2022-12-08 | Twist Bioscience Corporation | Polynucleotide libraries having controlled stoichiometry and synthesis thereof |
-
2017
- 2017-11-17 AU AU2017363204A patent/AU2017363204B2/en not_active Ceased
- 2017-11-17 EP EP17872347.4A patent/EP3541973B1/en active Active
- 2017-11-17 WO PCT/US2017/062391 patent/WO2018094263A1/en not_active Ceased
- 2017-11-17 US US15/816,995 patent/US20180142289A1/en not_active Abandoned
- 2017-11-17 IL IL266681A patent/IL266681B2/en unknown
- 2017-11-17 CA CA3044057A patent/CA3044057A1/en active Pending
- 2017-11-17 JP JP2019525959A patent/JP2020504709A/ja active Pending
- 2017-11-17 CN CN201780084031.1A patent/CN110382752A/zh active Pending
- 2017-11-17 IL IL314890A patent/IL314890A/en unknown
- 2017-11-17 GB GB1908719.6A patent/GB2572877A/en not_active Withdrawn
- 2017-11-17 KR KR1020197017281A patent/KR102569164B1/ko active Active
-
2021
- 2021-06-17 US US17/350,750 patent/US20210348220A1/en not_active Abandoned
-
2022
- 2022-10-28 JP JP2022173718A patent/JP2023017863A/ja active Pending
-
2023
- 2023-02-15 AU AU2023200850A patent/AU2023200850A1/en not_active Abandoned
-
2024
- 2024-10-31 JP JP2024192262A patent/JP2025028854A/ja active Pending
-
2025
- 2025-08-13 US US19/298,873 patent/US20260132447A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150056609A1 (en) * | 2012-05-09 | 2015-02-26 | Longhorn Vaccines And Diagnostics, Llc | Next Generation Genomic Sequencing Methods |
| US20160032396A1 (en) * | 2013-03-15 | 2016-02-04 | The Board Of Trustees Of The Leland Stanford Junior University | Identification and Use of Circulating Nucleic Acid Tumor Markers |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201908719D0 (en) | 2019-07-31 |
| CN110382752A (zh) | 2019-10-25 |
| IL266681A (en) | 2019-07-31 |
| IL314890A (en) | 2024-10-01 |
| WO2018094263A1 (en) | 2018-05-24 |
| JP2020504709A (ja) | 2020-02-13 |
| AU2017363204A1 (en) | 2019-07-04 |
| AU2017363204B2 (en) | 2022-12-08 |
| US20210348220A1 (en) | 2021-11-11 |
| EP3541973B1 (en) | 2024-03-27 |
| US20180142289A1 (en) | 2018-05-24 |
| IL266681B1 (en) | 2024-09-01 |
| JP2023017863A (ja) | 2023-02-07 |
| KR102569164B1 (ko) | 2023-08-21 |
| KR20190093585A (ko) | 2019-08-09 |
| EP3541973A4 (en) | 2020-07-29 |
| AU2023200850A1 (en) | 2023-03-16 |
| EP3541973A1 (en) | 2019-09-25 |
| CA3044057A1 (en) | 2018-05-24 |
| IL266681B2 (en) | 2025-01-01 |
| JP2025028854A (ja) | 2025-03-05 |
| US20260132447A1 (en) | 2026-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3844783C0 (en) | PNEUMATIC TARGET IRRADIATION SYSTEMS FOR RADIOISOTOPE PRODUCTION | |
| GB2613480B (en) | Methods for generating circular nucleic acid molecules | |
| BR112018072195A2 (pt) | amplificação de incompatibilidade otimizada multiplexado (moma) - pcr de tempo real para avaliar o câncer | |
| IL266681A (en) | Polynucleotide libraries with controlled stoichiometry and their synthesis | |
| IL253196A0 (en) | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using them | |
| GB2581599B8 (en) | Tagging nucleic acid molecules from single cells for phased sequencing | |
| WO2018200380A8 (en) | Compositions and methods for improving sample identification in indexed nucleic acid libraries | |
| MX2018006973A (es) | Anticuerpos anti-cd73 humanizados. | |
| EP4467654A3 (en) | Genome fractioning | |
| EP3704136C0 (en) | ADAPTER-BASED RETROVIRAL VECTOR SYSTEM FOR SELECTIVE TRANSDUCTION OF TARGET CELLS | |
| MX2018010362A (es) | Metodos y sistemas para evaluar carga mutacional de tumores. | |
| MX2018013224A (es) | Número de objetivo por amplificación de discordancias optimizada multiplicada (moma). | |
| EP3518656A4 (en) | PROCESS FOR SELECTING TARGET SITES FOR MODIFICATION OF SITE-SPECIFIC GENOME IN PLANTS | |
| EP3497221A4 (en) | COMPOSITIONS, SYSTEMS AND METHODS FOR PROGRAMMING IMMUNE CELL FUNCTION BY TARGETED GENE REGULATION | |
| WO2018094356A3 (en) | Compositions and methods for target nucleic acid modification | |
| SG10201809312QA (en) | Nucleic acid synthesis techniques | |
| EA201691811A1 (ru) | Варианты гфпд и способы их применения | |
| MX2016014313A (es) | Moleculas de acido nucleico dre4 que confieren resistencia a las plagas de coleopteros. | |
| GB2577654B (en) | Acid resistant urease inhibitor containing fertilizer compositions | |
| DK3870562T3 (da) | Hfo-1234ze- og hfo-1234yf-sammensætninger og processer til fremstilling og anvendelse af sammensætningerne | |
| DK3371313T3 (da) | Fremgangsmåder og sammensætninger til forbedret plantetransformation | |
| SG11202009774XA (en) | Compositions and methods for preparing nucleic acid libraries | |
| MX2016008261A (es) | Ras opuesta (rop) y moleculas de acido nucleico relacionadas que confieren resistencia a plagas de coleopteros y hemipteros. | |
| EP3789752A4 (en) | REACTION VESSEL FOR TESTING | |
| GB201918755D0 (en) | Control nucleic acids, and compositions kits, and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |