GB2422902A - Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes - Google Patents

Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes

Info

Publication number
GB2422902A
GB2422902A GB0610644A GB0610644A GB2422902A GB 2422902 A GB2422902 A GB 2422902A GB 0610644 A GB0610644 A GB 0610644A GB 0610644 A GB0610644 A GB 0610644A GB 2422902 A GB2422902 A GB 2422902A
Authority
GB
United Kingdom
Prior art keywords
restriction enzymes
dna sequences
hemimethylation
cytosine methylation
investigating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0610644A
Other languages
English (en)
Other versions
GB0610644D0 (en
Inventor
Joern Lewin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of GB0610644D0 publication Critical patent/GB0610644D0/en
Publication of GB2422902A publication Critical patent/GB2422902A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
GB0610644A 2003-11-09 2004-11-09 Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes Withdrawn GB2422902A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2003153419 DE10353419B3 (de) 2003-11-09 2003-11-09 Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme
PCT/EP2004/012853 WO2005045069A2 (fr) 2003-11-09 2004-11-09 Procede de controle de la methylation de la cytosine dans des sequences d'adn au moyen d'enzymes de restriction sensibles a l'hemi-methylation

Publications (2)

Publication Number Publication Date
GB0610644D0 GB0610644D0 (en) 2006-07-05
GB2422902A true GB2422902A (en) 2006-08-09

Family

ID=34559656

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0610644A Withdrawn GB2422902A (en) 2003-11-09 2004-11-09 Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes

Country Status (3)

Country Link
DE (1) DE10353419B3 (fr)
GB (1) GB2422902A (fr)
WO (1) WO2005045069A2 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
WO1999028501A1 (fr) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Systeme fluorometrique d'observation et de detection de l'amplification d'acides nucleiques
EP0976835A1 (fr) * 1998-07-29 2000-02-02 Keygene N.V. Méthode de détection de methylation des acides nucléiques par AFLP
EP1138782A2 (fr) * 2000-03-26 2001-10-04 Bruker-Saxonia Analytik Gmbh Variation multiplex de séquence pour l'analyse des échantillons d'ADN par spectrométrie de masse
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
WO2003023065A1 (fr) * 2001-09-06 2003-03-20 Syngenta Participations Ag Types de methylation de l'adn
EP1312685A2 (fr) * 2001-11-16 2003-05-21 Nisshinbo Industries, Inc. Matrice d'oligonucleotides pour la détection d'états de methylation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2412609C (fr) * 2000-06-23 2010-09-07 The University Of Chicago Methodes d'isolation d'adn centromere
EP1438428A4 (fr) * 2001-09-14 2005-11-16 Univ Queensland Detection de methylation d'adn
WO2003027640A2 (fr) * 2001-09-28 2003-04-03 University Of Delaware Détection et séparation de polymorphismes

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
WO1999028501A1 (fr) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Systeme fluorometrique d'observation et de detection de l'amplification d'acides nucleiques
EP0976835A1 (fr) * 1998-07-29 2000-02-02 Keygene N.V. Méthode de détection de methylation des acides nucléiques par AFLP
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
EP1138782A2 (fr) * 2000-03-26 2001-10-04 Bruker-Saxonia Analytik Gmbh Variation multiplex de séquence pour l'analyse des échantillons d'ADN par spectrométrie de masse
WO2003023065A1 (fr) * 2001-09-06 2003-03-20 Syngenta Participations Ag Types de methylation de l'adn
EP1312685A2 (fr) * 2001-11-16 2003-05-21 Nisshinbo Industries, Inc. Matrice d'oligonucleotides pour la détection d'états de methylation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Genome Research 12 (1) 2002. Gitan R S et.al. pages 158-164 *
Nucleic Acids Res. 26 (10) 1998. Rein et. al. pages 2255-2264 *

Also Published As

Publication number Publication date
WO2005045069A3 (fr) 2005-07-21
WO2005045069A2 (fr) 2005-05-19
DE10353419B3 (de) 2005-06-30
GB0610644D0 (en) 2006-07-05

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Legal Events

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)