GB0610644D0 - Method for investigating cytosine methylation in dna sequences by means of hemimethylation restriction enzymes - Google Patents

Method for investigating cytosine methylation in dna sequences by means of hemimethylation restriction enzymes

Info

Publication number
GB0610644D0
GB0610644D0 GBGB0610644.7A GB0610644A GB0610644D0 GB 0610644 D0 GB0610644 D0 GB 0610644D0 GB 0610644 A GB0610644 A GB 0610644A GB 0610644 D0 GB0610644 D0 GB 0610644D0
Authority
GB
United Kingdom
Prior art keywords
hemimethylation
dna sequences
restriction enzymes
cytosine methylation
investigating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GBGB0610644.7A
Other versions
GB2422902A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of GB0610644D0 publication Critical patent/GB0610644D0/en
Publication of GB2422902A publication Critical patent/GB2422902A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB0610644A 2003-11-09 2004-11-09 Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes Withdrawn GB2422902A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10353419A DE10353419B3 (en) 2003-11-09 2003-11-09 Method for studying cytosine methylations in DNA sequences using hemimethylation-sensitive restriction enzymes
PCT/EP2004/012853 WO2005045069A2 (en) 2003-11-09 2004-11-09 Method for investigating cytosine methylation in dna sequences by means of hemi-methylation sensitive restriction enzymes

Publications (2)

Publication Number Publication Date
GB0610644D0 true GB0610644D0 (en) 2006-07-05
GB2422902A GB2422902A (en) 2006-08-09

Family

ID=34559656

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0610644A Withdrawn GB2422902A (en) 2003-11-09 2004-11-09 Method for investigating cytosine methylation in DNA sequences by means of hemimethylation sensitive restriction enzymes

Country Status (3)

Country Link
DE (1) DE10353419B3 (en)
GB (1) GB2422902A (en)
WO (1) WO2005045069A2 (en)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
ATE244771T1 (en) * 1998-07-29 2003-07-15 Keygene Nv METHOD FOR DETECTING NUCLEIC ACID METHYLATIONS BY AFLP
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
DE10015797B4 (en) * 2000-03-26 2006-02-02 Bruker Daltonik Gmbh Multiplex analysis of DNA mixtures using photolytically readable DNA chips
CA2412609C (en) * 2000-06-23 2010-09-07 The University Of Chicago Methods for isolating centromere dna
WO2003023065A1 (en) * 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
EP1438428A4 (en) * 2001-09-14 2005-11-16 Univ Queensland Detection of dna methylation
AU2002341898A2 (en) * 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
JP2003144172A (en) * 2001-11-16 2003-05-20 Nisshinbo Ind Inc Oligonucleotide-immobilized board for detecting methylation

Also Published As

Publication number Publication date
DE10353419B3 (en) 2005-06-30
WO2005045069A3 (en) 2005-07-21
WO2005045069A2 (en) 2005-05-19
GB2422902A (en) 2006-08-09

Similar Documents

Publication Publication Date Title
GB2398383B (en) Method and means for nucleic acid sequencing
GB0514933D0 (en) Improved method of nucleotide detection
IL172093A0 (en) A plurality of nucleic acid molecules and methods for generating nucleotide sequences
AU2003304094A8 (en) Nucleic acid sequence detection using multiplexed oligonucleotide pcr
IL181410A0 (en) Process for plasmid dna fermentation
AU2003258452A8 (en) Rna interference based methods and compositions for inhibiting hbv gene expression
EP1727900A4 (en) Method for selectively separating and purifying rna and method for separating and purifying nucleic acid
AU2001242277A1 (en) Method for detecting cytosine methylation in DNA samples
EP1692266A4 (en) Improved methods for detecting and measuring specific nucleic acid sequences
GB0426100D0 (en) Method of detecting DNA variation in sequence data
TWI319436B (en) Method of detecting acid-fast bacteria using ribosomal rna as target
IL154270A0 (en) Method for detecting mutations in nucleotide sequences
GB0610644D0 (en) Method for investigating cytosine methylation in dna sequences by means of hemimethylation restriction enzymes
EP1688489A4 (en) Method of selecting genomic dna fragment
PL1737945T3 (en) Method for purifying plasmid dna
AU2003302685A1 (en) Methylation dna detecting method
EP1652917A4 (en) Rna capable of inhibiting expression of klf5 gene
AU2003233219A8 (en) Methods for identifying and isolating unique nucleic acid sequences
EP1698694A4 (en) Method for obtaining gene tag
EP1664282A4 (en) Method for isolating hepatocytes
EP1627060A4 (en) Process for extracting nucleic acid
GB2415782B8 (en) Method for obtaining aptamer using microarrays
EP1641934A4 (en) Methods for enhancing gene expression analysis
AU2003215083A1 (en) Method and apparatus for validating dna sequences without sequencing
GB0405925D0 (en) Method for simultaneous detection of multiple nucleic acid sequences

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)