GB2361238A - Human genome-derived single exon nucleic acid probes - Google Patents

Abstract

A single exon nucleic acid microarray comprising a plurality of single exon nucleic acid probes for measuring gene expression in a sample derived from human heart is described. Also described are single exon nucleic acid probes expressed in the heart and their use in methods for detecting gene expression.

Description

2361238 1 HUMAN GENOME-DERIVED SINGLE EXON NUCLEIC ACID PROBES USEFUL FOR

ANALYSIS OF GENE EXPRESSION IN HUMAN HEART Crossreference to parent application.

The present application is a divisional application of GB 0024263.6. All references to SEQ ID N0s. in the present application are references to the sequence listing accompanying GB 0024263.6.

Field of the Invention

The present invention relates to genome-derived single exon microarrays useful for verifying the expression of regions of genomic DNA predicted to encode protein. In particular, the present invention relates to unique genomederived single exon nucleic acid probes expressed in human heart and single exon nucleic acid microarrays that include such probes.

Background of the Invention

For almost two decades following the invention of general techniques for nucleic acid sequencing, Sanger et al., Proc. Natl. Acad. Sci. USA 70 (4):12 09-13 (1973); Gilbert et al., Proc. Natl. Acad. Scl. USA 70(12):3581-4 (1973), these techniques were used principally as tools to further the understanding of proteins - known or suspected - about which a basic foundation of biological knowledge had already been built. In many cases, the cloning effort that preceded sequence identification had been both informed and directed by that antecedent biological understanding.

For example, the cloning of the T cell receptor 2 for antigen was predicated upon its known or suspected cell type- specific expression, by its suspected membrane association. and by the predicted assembly of its gene via T cell-specific somatic recombination. Subsequent sequencing efforts at once confirmed and extended understanding of this family of proteins. Hedrick et al., Nature 308 (5955):153-8 (1984).

More recently, however, the development of high throughput sequencing methods and devices, in concert with large public and private undertakings to sequence the human and other genomes, has altered this investigational paradigm: today, sequence information often precedes understanding of the basic biology of the encoded protein product.

one of the approaches to large-scale sequencing is predicated upon the proposition that expressed sequences - that is, those accessible through isolation of mRNA - are of greatest initial interest. This "expressed sequence tag" (I1EST11) approach has already yielded vast amounts of sequence data (see for example Adams et al., Science 252:1651 (1991); Williamson, Drug Discov. Today 4:115 (1999)). For nucleic acids sequenced by this approach, often the only biological information that is known a priori with any certainty is the likelihood of biologic expression itself. By virtue of the species and tissue from which the mRNA had originally been obtained, most such sequences are also annotated with the identity of the species and at least one tissue in which expression appears likely.

More recently, the pace of genomic sequencing has accelerated dramatically. When genomic DNA serves as the initial substrate for sequencing efforts, expression cannot be presumed; often the only a priori biological information about the sequence includes the species and chromosome (and 3 perhaps chromosomal map location) of origin.

With the ever-accelerating pace of sequence accumulation by directed, EST, and genomic sequencing approaches - and in particular, with the accumulation of sequence information from multiple genera, from multiple species within genera, and from multiple individuals within a species there is an increasing need for methods that rapidly and effectively permit the functions of nucleic sequences to be elucidated. And as such functional information accumulates, there is a further need for methods of storing such functional information in meaningful and useful relationship to the sequence itself; that is, there is an increasing need for means and apparatus for annotating raw sequence data with known or predicted functional information.

Although the increase in the pace of genomic sequencing is due in large part to technological changes in sequencing strategies and instrumentation, Service, Science 280:995 (1998); Pennisi, Science 283: 18221823 (1999) there is an important functional motivation as well.

While it was understood that the EST approach would rarely be able to yield sequence information about the noncoding portions of the genome, it now also appears the EST approach is capable of capturing only a fraction of a genomels actual expression complexity.

For example, when the C. elegans genome was fully sequenced, gene prediction algorithms identified over 19,000 potential genes, of which only 7,000 had been found by EST sequencing. C. elegans Sequencing Consortium, Science 282:2012 (1998). Analogously, the recently completed sequence of chromosome 2 of Arabidopsis predicts over 4000 genes, Lin et al., Nature, 402:761 (1999), of which only about 6% had previously been identified via EST sequencing efforts. Although the human genome has the 4 greatest depth of EST coverage, it is still woefully short of surrendering all of its genes. One recent estimate suggests that the human genome contains more than 146,000 genes, which would at this point leave greater than half of the genes undiscovered. It is now predicted that many genes, perhaps 20 to 50%, will only be found by genomic sequencing.

There is, therefore, a need for methods that permit the functional regions of genomic sequence - and most importantly, but not exclusively, regions that function to encode genes - to be identified.

Much of the coding sequence of the human genome is not homologous to known genes, making detection of open reading frames P1ORFs11) and predictions of gene function difficult. Computational methods exist for predicting coding regions in eukaryotic genomes. Gene prediction programs such as GRAIL and GRAIL II, Uberbacher et al., Proc. Natl. Acad. Sci. USA 88 (24):11261-5 (1991); Xu et al., Genet. Eng. 16:241-53 (1994); Uberbacher et: al., Methods Enzymol. 266:259-81 (1996); GENEFINDER, Solovyev et al., Nucl. Acids. Res. 22:5156-63 (1994); Solovyev et al., Ismb 5:294302 (1997); and GENESCAN, Burge et al., J. Mol. Biol. 268:78-94 (1997), predict many putative genes without known homology or function. Such programs are known, however, to give high false positive rates. Burset et al., Genomics 34:353-367 (1996). Using a consensus obtained by a plurality of such programs is known to increase the reliability of calling exons f rom genomic sequence.

Ansari-Lari et al., Genome Res. 8 (1):29-40 (1998) Identification of functional genes from genomic data remains, however, an imperfect art. For example, in reporting the full sequence of human chromosome 21, the Chromosome 21 Mapping and Sequencing Consortium reports that prior bioinformatic estimates of human gene number may need to be revised substantially downwards. Nature 405:311-199 (2000); Reeves, Nature 405:283-284 (2000) Thus, there is a need for methods and apparatus that permit the functions of the regions identified bioinformatically - and specifically, that permit the expression of regions predicted to encode protein - readily to be confirmed experimentally.

Recently, the development of nucleic acid microarrays has made possible the automated and highly 10 parallel measurement of gene expression. Reviewed in Schena (ed.), DNA Microarrays: A Practical Approach (Practical Approach Series), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21 (1) (suppl):1 - 60 (1999); Schena (ed.), Microarray Biochip: Tools and Technology, Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376).

It is common for microarrays to be derived from cDNA/EST libraries, either from those previously described in the literature, such as those from the I.M.A.G.E.

consortium, Lennon et al., Genomics 33 (1):151-2 (1996), or from the construction of "problem specific,, libraries targeted at a particular biological question, R.S. Thomas et al., Cancer Res. (in press). Such microarrays by definition can measure expression only of those genes found in EST libraries, and thus have not been useful as probes for genes discovered solely by genomic sequencing.

The utility of using whole genome nucleic acid microarrays to answer certain biological questions has been demonstrated for the yeast Saccharomyces cerevisiae. De Risi et al., Science 278:680 (1997). The vast majority of yeast nuclear genes, approximately 95% however, are single exon genes, i.e., lack introns, Lopez et al., RNA 5:1135- 1137 (1999); Goffeau et al., Science 274:563-67 (1996), permitting coding regions more readily to be identified.

6 Whole genome nucleic acid microarrays have not generally been used to probe gene expression from more complex eukaryotic genomes, and in particular from those averaging more than one intron per gene.

Summary of the Invention

The present invention solves these and other problems in the art by providing methods and apparatus for predicting, confirming, and displaying functional information derived from genomic sequence. The present invention also provides apparatus for verifying the expression of putative genes identified within genomic sequence.

In particular, the invention provides novel genome-derived single exon nucleic acid microarrays useful for verifying the expression of putative genes identified within genomic sequence.

The present invention also provides compositions and kits for the ready production of nucleic acids identical in sequence to, or substantially identical in sequence to, probes on the genome- derived single exon microarrays of the present invention.

Accordingly, in a first aspect of the invention, there is provided a spatial ly- addressable set of single exon nucleic acid probes for measuring gene expression in a sample derived from human heart, comprising a plurality of single exon nucleic acid probes according to any one of the nucleotide sequences set out in SEQ ID NOs: 1 - 5,202 of the sequence listing accompanying GB 0024263.6 or a complementary sequence, or a portion of such a sequence.

By plurality is meant at least two, suitably at least 20, most suitably at least 100, preferably at least 1000 and, most preferably, upto 5000.

7 In one embodiment of the f irst aspect, each of said plurality of probes is separately and addressably amplifiable.

In an alternative embodiment, each of said plurality of probes is separately and addressably isolatable from said plurality.

In a preferred embodiment, each of said plurality of probes is amplifiable using at least one common primer.

Preferably, each of said plurality of probes is amplifiable using a first and a second common primer.

In yet another embodiment, said set of single exon nucleic acid probes comprises between 50 - 20,000 probes, for example, 50 - 5000.

Suitably, said set of single exon nucleic acid probes comprises at least 50 1000 discrete single exon nucleic acid probes having a sequence as set out in any of SEQ ID NOS.: 1 - 10,283 of the sequence listing accompanying GB 0024263.6 or a complimentary sequence, or a portion of such a sequence.

Preferably, the average length of the single exon nucleic acid probes is between 200 and 500 bp. It is preferred that the average length should be at least 200bp, suitably at least 250bp, most suitably at least 300bp, preferably at least 400bp and, most preferably, 500 bp.

In another embodiment, the single exon nucleic acid probes lack prokaryotic and bacteriophage vector sequence. It is preferred that at least 50%, suitably at least 60%, most suitably at least 70%, preferably at least 75%, more preferably at least 80, 85, 90, 95 or 99% of said single exon nucleic acid probes lack prokaryotic and bacteriophage vector sequence.

In another preferred embodiment, said single exon nucleic acid lack homopolymeric stretches of A or T. It is preferred that at least 50%, suitably at least 60%, most 8 suitably at least 70%, preferably at least 75%, more preferably at least 80, 85, 90, 95 or 99% of said single exon nucleic acid probes lack homopolymeric stretches of or T.

In a particularly preferred embodiment, a set of single exon nucleic acid probes in accordance with a first aspect of the invention comprises a nucleotide sequence selected from any of SEQ ID NOS.: 286, 287, 442, 871, 1029, 1280, 1597, 1619, 1694, 1715, 1721, 1848, 1935, 2119, 2233, 2893, 3003, 3003, 3149, 3403,3471, 4184, 4184, 4242, 4730, 4779, 4976, 6169, 6410, 6723, 6745, 6818, 6838, 6844, 6966, 7052, 7230 7340, 8044, 8155, 8298, 8545, 8612, 8654, 8746, 9228, 9290 9310, 9367, 9842, 9889, 10079 and 10091 of the sequence listing accompanying GB 0024263.6, a sequence complementary to any of said sequences, or a portion of such a sequence. In yet another embodiment, a set of single exon 3513, 3607, 4102, 4164, 4988, 5473, 5608, 6019, nucleic acid probes in accordance with the invention comprises a nucleotide encoding a peptide having an amino acid sequence as set out in any of SEQ ID NOS.: 10603, 10741, 11179, 11571, 11893, 11913, 11999, 12017, 12023, 12152, 12245, 12439, 12552, 13144, 13255, 13256, 13407, 13653, 13724, 13841, 14313, 14370, 14390, 14391, 14934, 15149 and 15161, of the sequence listing accompanying GB 0024263.6 or portion thereof.

Preferably, a spatially-addressable set of single exon nucleic acid probes in accordance with the first aspect of the invention is is addressably disposed upon a substrate.

Suitable substrates include a filter membrane which may, preferably, be nitrocellulose or nylon. The nylon may preferably, be positively-charged. Other suitable substrates include glass, amorphous silicon, crystalline silicon, and plastic. Further suitable materials include 9 polymethylacrylic, polyethylene, polypropylene, polyacrylate, polymethylmethacrylate, polyvinyl chloride, polytetrafluoroethylene, polystyrene, polycarbonate, polyacetal, polysulfone, celluloseacetate, cellulosenitrate, nitrocellulose, and mixtures thereof.

In a second aspect of the invention, there is provided a microarray comprising a spatially addressable set of single exon nucleic acid probes in accordance with the first aspect of the invention.

In one embodiment, a genome-derived single-exon microarray is packaged together with such an ordered set of amplifiable probes corresponding to the probes, or one or more subsets of probes, thereon. In alternative embodiments, the ordered set of amplifiable probes is packaged separately from the genome-derived single exon microarray.

In another aspect, the invention provides genomederived single exon nucleic acid probes useful for gene expression analysis, and particularly for gene expression analysis by microarray. In particular embodiments of this aspect, the present invention provides human single-exon probes that include spec if ical ly-hybridi zable fragments of SEQ ID Nos. 5,203 10,283, wherein the fragment hybridizes at high stringency to an expressed human gene. In particular embodiments, the invention provides single exon probes comprising SEQ ID Nos. 1 - 5,202.

Accordingly, in a third aspect of the invention, there is provided a single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart which is a nucleic acid molecule comprising a nucleotide sequence as set out in any of SEQ ID N0s.: 1 5,202 or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human heart.

In one embodiment, a single exon nucleic acid probe in accordance with the third aspect comprises a nucleotide sequence as set out in any of SEQ ID N0s.: 5,203 - 10,283 or a complementary sequence or a fragment thereof.

In a fourth aspect of the invention, there is provided a single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart which is a nucleic acid molecule having a sequence encoding a peptide comprising a peptide sequence as set out in any of SEQ ID N0s.: 10, 284 - 15, 322 of the sequence listing accompanying GB 0024263.6, or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human heart.

In a preferred embodiment, a single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart is selected from any of SEQ ID NOS.:

1715, 3403, 4779, 6818, 8545, 8612, 8654, 8746, 9228, 9290, 9310, 9367, 9842, 9889, 10079 and 10091 of the sequence listing accompanying GB 0024263.6.

In another embodiment, a single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart is selected from a nucleotide encoding a peptide, or portion thereof, as set out in any of SEQ ID NOS.: 10603, 10741, 11179, 11571, 11893, 11913, 11999, 12017, 12023, 12152, 12245, 12439, 12552, 13144, 13255, 13256, 13407, 13653, 13724, 13841, 14313, 14370, 14390, 14391, 14934, 15149 and 15161 of the sequence listing accompanying GB 0024263.6.

Preferably, a single exon nucleic acid probe in 286, 287, 442, 871, 1029, 1280, 1597, 1619, 1694, 1721, 1848, 1935, 2119, 2233, 2893, 3003, 3003, 3149, 3471, 3513, 3607, 4102, 4164, 4184, 4184, 4242, 4730, 4976, 4988, 5473, 5608, 6019, 6169, 6838, 6844, 6966, 7052, 7230, 7340, 6410, 6723, 8044, 8155, 6745, 8298, 11 accordance with the third or fourth aspects of the invention comprises between at least 15 and 50 contiguous nucleotides of said SEQ ID NO:. It is preferred that the single exon nucleic acid probe comprises at least 15, suitably at least 20. more suitably at least 25 or preferably at least 50 contiguous nucleotides of said SEQ ID NO:.

In another preferred embodiment, a single exon nucleic acid probe in accordance with the third or fourth aspects of the invention is between 3kb and 25kb in length. It is preferred that said probe is no more than 3kb, suitably no more than 5kb, more suitably no more than 10kb, preferably 15kb, more preferably 20kb or, most preferably, no more than 20kb in length.

Preferably, a single exon nucleic acid probe in accordance with either the fifth or sixth aspect of the invention is DNA, preferably single-stranded DNA, RNA or PNA.

In another embodiment of either the third or fourth aspect of the invention, a single exon nucleic acid probe is detectably labeled. Suitable detectable labels include a radionuclide, a fluorescent label or a first member of a specific binding pair. Suitable fluorescent labels include dyes such as cyanine dyes, preferably Cy3 and Cy5 although other suitable dyes will be known to those skilled in the art.

In a particularly preferred embodiment, a single exon nucleic acid probe in accordance with either the third or fourth aspect of the invention lacks prokaryotic and bacteriophage vector sequence. In yet another embodiment, a single exon nucleic acid probe in accordance with either the third or fourth aspect of the invention lacks homopolymeric stretches of A or T.

In a fifth aspect of the invention, there is 12 provided an amplifiable nucleic acid composition, comprising:

the single exon nucleic acid probe in accordance with either of the third or fourth aspects of the invention; and at least one nucleic acid primer; wherein said at least one primer is sufficient to prime enzymatic amplification of said probe.

In an sixth aspect of the invention, there is provided a method of measuring gene expression in a sample 10 derived from human heart, comprising:

contacting the single exon microarray in accordance with the second aspect of the invention, with a first collection of detectably labeled nucleic acids, said first collection of nucleic acids derived from mRNA of human heart; and then measuring the label detectably bound to each probe of said microarray.

In a seventh aspect of the invention, there is provided a method of identifying exons in a eukaryotic genome, comprising:

algorithmically predicting at least one exon from genomic sequence of said eukaryote; and then detecting specific hybridization of detectably labeled nucleic acids to a single exon probe, wherein said detectably labeled nucleic acids are derived from mRNA from the heart of said eukaryote, said probe is a single exon probe having a fragment identical in sequence to, or complementary in sequence to, said predicted exon, said probe is included within a single exon microarray in accordance with the first aspect of the invention, and said fragment is selectively hybridizable at high stringency.

In a eighth aspect of the invention, there is provided a method of assigning exons to a single gene, 13 comprising:

identifying a plurality of exons from genomic sequence in accordance with the seventh aspect of the invention; and then measuring the expression of each of said exons in a plurality of tissues and/or cell types using hybridization to single exon microarrays having a probe with said exon, wherein a common pattern of expression of said exons in said plurality of tissues and/or cell types indicates that the exons should be assigned to a single gene.

In an ninth aspect of the invention, there is provided a nucleic acid sequence as set out in any of SEQ ID NOs: 1 - 10,283 wherein said sequence encodes a peptide.

In a tenth aspect of the invention, there is provided a peptide encoded by a sequence comprising a sequence as set out in any of SEQ ID NOs: 5,203 10,283, or a complementary sequence or coding portion thereof.

In a preferred embodiment, a peptide may be encoded by a sequence comprising a sequence set out in any of SEQ ID NOS.: 1 -5,202.

In a further aspect, the invention provides peptides comprising an amino acid sequence translated from the DNA fragments, said amino acid sequences comprising SEQ ID NOS.: 10,283 - 15,322.

Accordingly in a eleventh aspect of the invention there is provided a peptide comprising a sequence as set out in any of SEQ ID NOs: 10,284 15,322, or fragment thereof.

In another aspect, the invention provides means for displaying annotated sequence, and in particular, for displaying sequence annotated according to the methods and apparatus of the present invention. Further, such display 14 can be used as a preferred graphical user interface for electronic search, query, and analysis of such annotated sequence.

D Detailed Description of the Invention

Definitions As used herein, the term Ilmicroarrayll and phrase "nucleic acid microarrayll refer to a substrate-bound collection of plural nucleic acids, hybridization to each of the plurality of bound nucleic acids being separately detectable. The substrate can be solid or porous, planar or non-planar, unitary or distributed.

As so defined, the term Ilmicroarrayll and phrase "nucleic acid microarrayll include all the devices so called in Schena (ed.), DNA Microarrays: A Practical Approach (Practical Approach Series), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21 (1) (suppl):1 - 60 (1999); and Schena (ed.), Microarray Biochip: Tools and Technology, Eaton Publishing Company/BioTechniques, Books Division (2000) (ISBN: 1881299376). As so defined, the term Ilmicroarrayll and phrase "nucleic acid microarrayll further include substrate-bound collections of plural nucleic acids in which the nucleic acids are distributably disposed on a plurality of beads, rather than on a unitary planar substrate, as is described, inter alia, in Brenner et al., Proc. Natl. Acad. Sci. USA 97 (4):166501670 (2000) in such case, the term Ilmicroarrayll and phrase "nucleic acid microarrayll refer to the plurality of beads in aggregate.

As used herein with respect to a nucleic acid microarray, the term "probe" refers to the nucleic acid that is, or is intended to be, bound to the substrate; in such context, the term "target" thus refers to nucleic acid intended to be bound thereto by Watson-Crick complementarity. As used herein with respect to solution phase hybridization, the term "probe" refers to the nucleic 5 acid of known sequence that is detectably labeled.

As used herein, the expression "probe comprising SEQ ID NO.", and variants thereof, intends a nucleic acid probe, at least a portion of which probe has either (i) sequence directly as given in the referenced SEQ ID NO., or (ii) a sequence complementary to the sequence as given in the referenced SEQ ID NO., the choice as between sequence directly as given and complement thereof dictated by the requirement that the probe hybridize to mRNA.

As used herein, the term 11ORF11 refers to a nucleic acid sequence that, in at least one reading frame, does not possess stop codons; the term does not require that the ORF encode the entirety of a natural protein. In the absence of a consensus prediction, all six frames of an exon are examined for stop codons and the longest ORF is selected.

As used herein, the term "amplicon" refers to a PCR product amplified from human genomic DNA, containing the predicted exon.

As used herein the term "exon" refers to the consensus prediction of the various exon and gene predicting algorithms.

As used herein, the term "peptide" refers to a sequence of amino acids. The sequences referred to as PEPTIDE SEQ ID NOS.: are the predicted peptide sequences that would be translated f rom one of the exons, or a portion thereof set out in exon SEQ ID NOS.:. The codons encoding the peptide are wholly contained within the exon.

As used herein, a "portions" of a def ined nucleotide sequence or sequences can be and, preferably, 16 are fragments unique to that sequence or to one or a combination of those sequences. A fragment unique to a nucleic acid molecule is one that is a signature for the larger nucleic acid molecule.

As used herein, the phrase "expression of a probe" and its linguistic variants means that the ORF present within the probe, or its complement, is present within a target mRNA.

As used herein, -stringent conditions" refers to parameters well known to those skilled in the art. When a nucleic acid molecule is said to be hybridisable to another of a given sequence under "stringent conditions" it is meant that it is homologous to the given sequence.

As used herein, the phrase "specific binding pair,, intends a pair of molecules that bind to one another with high specificity. Binding pairs are said to exhibit specific binding when they exhibit avidity of at least 107, preferably at least 108, more preferably at least 109 liters/mole. Nonlimiting examples of specific binding pairs are: antibody and antigen; biotin and avidin; and biotin and streptavidin.

As used herein with respect to the visual display of annotated genomic sequence, the term "rectangle" means any geometric shape that has at least a first and a second border, wherein the first and second borders each are capable of mapping uniquely to a point of another visual object of the display.

As used herein, a 11Mondrianll means a visual display in which a single genomic sequence is annotated with predicted and experimentally confirmed functional information.

Brief Description of the Drawings

17 The present invention is further illustrated with reference to the following non-limiting figures and examples in which:

FIG. 1 illustrates a process for predicting functional regions from genomic sequence, confirming the functional activity of such regions experimentally, and associating and displaying the data so obtained in meaningful and useful relationship to the original sequence data; FIG. 2 further elaborates that portion of the process schematized in FIG. 1 for predicting functional regions from genomic sequence; FIG. 3 illustrates a Mondrian visual display; FIG. 4 presents a Mondrian showing a hypothetical annotated genomic sequence; FIG. 5 is a histogram showing the distribution of ORF length and PCR products as obtained, with ORF length shown in black and PCR product length shown in dotted lines; FIG. 6 is a histogram showing the distribution, among exons predicted according to the methods described, of expression as measured using simultaneous two color hybridization to a genome-derived single exon microarray. The graph showsthe number of sequence veri f ied products that were either not expressed (1,011), expressed in one or more but not all tested tissues (11111 11911), or expressed in all tissues tested (111011); FIG. 7 is a pictorial representation of the expression of verified sequences that showed expression with signal intensity greater than 3 in at least one tissue, with: FIG. 7A showing the expression as measured by microarray hybridization in each of the 10 measured tissues, and the expression as measured llbioinformaticallyll 18 by query of EST, NR and SwissProt databases; with FIG. 7B showing the legend for display of physical expression (ratio) in FIG. 7A; and with FIG. 7C showing the legend for scoring EST hits as depicted in FIG. 7A; FIG. 8 shows a comparison of normalized CY3 signal intensity for arrayed sequences that were identical to sequences in existing EST, NR and SwissProt databases or that were dissimilar (unknown), where black denotes the signal intensity for all sequence-verified products with a BLAST Expect (11E11) value of greater than le-30 (1 X 10-30) Punknown") and a dotted line denotes sequence -verified spots with a BLAST expect (11E11) value of less than le-30 (1 X 10-30) ("known],); FIG. 9 presents a Mondrian of BAC AC008172 (bases 25,000 to 130,000), containing the carbamyl phosphate synthetase gene (AF154830.1); and FIG. 10 is a Mondrian of BAC A049839.

Methods and Apparatus for Predicting, Confirming, Annotating, and Displaying Functional Regions From Genomic Sequence Data FIG. 1 is a flow chart illustrating in broad outline a process for predicting functional regions from genomic sequence, confirming and characterizing the functional activity of such regions experimentally, and then associating and displaying the information so obtained in meaningful and useful relationship to the original sequence data.

The initial input into process 10 of the present invention is drawn from one or more databases 100 containing genomic sequence data. Because genomic sequence is usually obtained from subgenomic fragments, the sequence 19 data typically will be stored in a series of records corresponding to these subgenomic sequenced fragments. Some fragments will have been catenated to form larger contiguous sequences (Ilcontigs"); others will not. A finite percentage of sequence data in the database will typically be erroneous, consisting inter alia of vector sequence. sequence created from aberrant cloning events, sequence of artificial polylinkers, and sequence that was erroneously read.

Each sequence record in database 100 will minimally contain as annotation a unique sequence identifier (accession number), and will typically be annotated further to identify the date of accession, species of origin. and depositor. Because database 100 can contain nongenomic sequence, each sequence will typically be annotated further to permit query for genomic sequence. Chromosomal origin, optionally with map location, can also be present. Data can be, and over time increasingly will be, further annotated with additional information, in part through use of the present invention, as described below. Annotation can be present within the data records, in information external to database 100 and linked to the records thereto, or through a combination of the two.

Databases useful as genomic sequence database 100 in the present invention include GenBank, and particularly include several divisions thereof, including the htgs (draft), NT (nucleotide, command line), and NR (nonredundant) divisions. GenBank is produced by the National Institutes of Health and is maintained by the National Center for Biotechnology Information (NCBI). Databases of genomic sequence from species other than human, such as mouse, rat, Arabidopsis, C. elegans, C. brigsii, Drosophila, zebra fish, and other higher eukaryotic organisms will also prove useful as genomic sequence database 100.

Genomic sequence obtained by query of genomic sequence database 100 is then input into one or more processes 200 for identification of regions therein that are predicted to have a biological function as specified by the user. Such functions include, but are not limited to, encoding protein, regulating transcription, regulating message transport after transcription into mRNA, regulating message splicing after transcription into mRNA, of regulating message degradation after transcription into mRNA, and the like. other functions include directing somatic recombination events, contributing to chromosomal stability or movement, contributing to allelic exclusion or X chromosome inactivation, and the like.

The particular genomic sequence to be input into process 200 will depend upon the function for which relevant sequence is to be identified as well as upon the approach chosen for such identification. Process step 200 can be iterated to identify different functions within a given genomic region. In such case, the input often will be different for the several iterations.

Sequences predicted to have the requisite function by process 200 are then input into process 300, where a subset of the input sequences suitable for experimental confirmation is identified. Experimental confirmation can involve physical and/or bioinformatic assay. Where the subsequent experimental assay is bioinformatic, rather than physical, there are fewer constraints on the sequences that can be tested, and in this latter case therefore process 300 can output the entirety of the input sequence.

The subset of sequences output from process 300 is then used in process 400 for experimental verification and characterization of the function predicted in 21 process 200, which experimental verification can, and often will, include both physical and bioinformatic assay.

Process 500 annotates the sequence data with the functional information obtained in the physical and/or bioinformatic assays of process 400. Such annotation can be done using any technique that usefully relates the functional information to the sequence, as, for example, by incorporating the functional data into the sequence data record itself, by linking records in a hierarchical or relational database. by linking to external databases, by a combination thereof, or by other means well known within the database arts. The data can even be submitted for incorporation into databases maintained by others, such as GenBank, which is maintained by NCBI.

As further noted in FIG. 1, additional annotation can be input into process 500 from external sources 600.

The annotated data is then displayed in process 800, either before, concomitantly with, or after optional storage 700 on nontransient media, such as magnetic disk, optical disc, magnetooptical disk, flash memory, or the like.

FIG. 1 shows that the experimental data output from process 400 can be used in each preceding step of process 10: e.g., facilitating identification of functional sequences in process 200, facilitating identification of an experimentally suitable subset thereof in process 300, and facilitating creation of physical and/or informational substrates for, and performance of subsequent assay, of functional sequences in process 400.

Information from each step can be passed directly to the succeeding process, or stored in permanent or interim form prior to passage to the succeeding process. Often, data will be stored after each, or at least a plurality, of such process steps. Any or all process steps 22 can be automated.

FIG. 2 further elaborates the prediction of functional sequence within genomic sequence according to process 200.

Genomic sequence database 100 is first queried 20 for genomic sequence.

The sequence required to be returned by query 20 will depend, in the first instance, upon the function to be identified.

For example, genomic sequences that function to encode protein can be identified inter alla using gene prediction approaches, comparative sequence analysis approaches, or combinations of the two. In gene prediction analysis, sequence from one genome is input into process 200 where at least one, preferably a plurality, of algorithmic methods are applied to identify putative coding regions. In comparative sequence analysis, by contrast, corresponding, e.g., syntenic, sequence from a plurality of sources, typically a plurality of species, is input into process 200, where at least one, possibly a plurality, of algorithmic methods are applied to compare the sequences and identify regions of least variability.

The exact content of query 20 will also depend upon the database queried. For example, if the database contains both genomic and nongenomic sequence, perhaps derived from multiple species, and the function to be determined is protein coding regions in human genomic sequence, the query will accordingly require that the sequence returned be genomic and derived from humans.

Query 20 can also incorporate criteria that compel return of sequence that meets operative requirements of the subsequent analytical method. Alternatively, or in addition, such operative criteria can be enforced in subsequent preprocess step 24.

23 For example, if the function sought to be identified is protein coding, query 20 can incorporate criteria that return from genomic sequence database 100 only those sequences present within contigs sufficiently long as to have obviated substantial fragmentation of any given exon among a plurality of separate sequence fragments.

Such criteria can, for example, consist of a required minimal individual genomic sequence fragment length, such as 10 kb, more typically 20 kb, 30 kb, 40kb, and preferably 50 kb or more, as well as an optional further or alternative requirement that sequence from any given clone, such as a bacterial artificial chromosome (11BACII), be presented in no more than a finite maximal number of fragments, such as no more than 20 separate pieces, more typically no more than 15 fragments, even more typically no more than about 10 - 12 fragments.

Results using the present invention have shown that genomic sequence from bacterial artificial chromosomes (BACs) is sufficient for gene prediction analysis according to the present invention if the sequence is at least 50 kb in length, and if additionally the sequence from any given BAC is presented in fewer than 15, and preferably fewer than 10, fragments. Accordingly, query 20 can incorporate a requirement that data accessioned from BAC sequencing be in fewer than 15, preferably fewer than 10, fragments.

An additional criterion that can be incorporated into the query can be the date, or range of dates, of sequence accession. Although the process has been described above as if genomic sequence database 100 were static, it is of course understood that the genomic sequence databases need not be static, and indeed are typically updated on a frequent, even hourly, basis. Thus, as further described in Examples 1 and 2, infra, it is 24 possible to query the database for newly added sequence, either newly added after an absolute date, or newly added relative to a prior analysis performed using the methods and apparatus of the present invention. In this way, the process herein described can incorporate a dynamic, temporal component.

one utility of such temporal limitation is to identify, from newly accessioned genomic sequence, the presence of novel genes, particularly those not previously identified by EST sequencing (or other sequencing efforts that are similarly based upon gene expression). As further described in Example 1, such an approach has shown that newly accessioned human genomic sequence, when analyzed for sequences that function to encode protein, readily identifies genes that are novel over those in existing EST and other expression databases. This makes the methods of the present invention extremely powerful gene discovery tools. And as would be appreciated, such gene discovery can be performed using genomic sequence from species other than human.

If query 20 incorporates multiple criteria, such as above-described, the multiple criteria can be performed as a series of separate queries or as a single query, depending in part upon the query language, the complexity of the query, and other considerations well known in the database arts.

If query 20 returns no genomic sequence meeting the query criteria, the negative result can be reported by process 22, and process 200 (and indeed, entire process 10) ended 23, as shown. Alternatively, or in addition to report and termination of the initial inquiry, a new query 20 can be generated that takes into account the initial negative result.

When query 20 returns sequence meeting the query criteria, the returned sequence is then passed to optional preprocessing 24, suitable and specific for the desired analytical approach and the particular analytical methods thereof to be used in process 25.

Preprocessing 24 can include processes suitable for many approaches and methods thereof, as well as processes specifically suited for the intended subsequent analysis.

Preprocessing 24 suitable for most approaches and methods will include elimination of sequence irrelevant to, or that would interfere with, the subsequent analysis. Such sequence includes repetitive sequence, such as Alu repeats and LINE elements, vector sequence, artificial sequence, such as artificial polylinkers, and the like. Such removal can readily be performed by identification and subsequent masking of the undesired sequence.

Identification can be effected by comparing the genomic sequence returned by query 20 with public or private databases containing known repetitive sequence, vector sequence, artificial sequence, and other artifactual sequence. Such comparison can readily be done using programs well known in the art, such as CROSS - MATCH, or by proprietary sequence comparison programs the engineering of which is well within the skill in the art.

Alternatively, or in addition, undesirable including artifactual, sequence can be identified algorithmically without comparison to external databases and thereafter removed. For example, synthetic polylinker sequence can be identified by an algorithm that identifies a significantly higher than average density of known restriction sites. As another example, vector sequence can be identified by algorithms that identify nucleotide or codon usage at variance with that of the bulk of the genomic sequence.

26 once identified, undesired sequence can be removed. Removal can usefully be done by masking the undesired sequence as, for example, by converting the specific nucleotide references to one that is unrecognized by the subsequent bioinformatic algorithms, such as,X,,. Alternatively, but at present less preferred, the undesired sequence can be excised from the returned genomic sequence, leaving gaps.

Preprocessing 24 can further include selection from among duplicative sequences of that one sequence of highest quality. Higher quality can be measured as a lower percentage of, fewest number of, or least densely clustered occurrence of ambiguous nucleotides, defined as those nucleotides that are identified in the genomic sequence using symbols indicating ambiguity. Higher quality can also or alternatively be valued by presence in the longest contig.

Preprocessing 24 can, and often will, also include formatting of the data as specifically appropriate for passage to the analytical algorithms of process 25. Such formatting can and typically will include, inter alia, addition of a unique sequence identifier, either derived from the original accession number in genomic sequence database 100, or newly applied, and can further include additional annotation. Formatting can include conversion from one to another sequence listing standard, such as conversion to or from FASTA or the like, depending upon the input expected by the subsequent process.

Preprocessing, which can be optional depending upon the function desired to be identified and the informational requirements of the methods for effecting such identification, is followed by sequence processing 25, where sequences with the desired function are identified within the genomic sequence.

27 As mentioned above, such functions can include, but are not limited to, encoding protein, regulating transcription. regulating message transport after transcription into mRNA, regulating message splicing after transcription, of regulating message degradation, and the like. other functions include directing somatic recombination events, contributing to chromosomal stability or movement, contributing to allelic exclusion or X chromosome inactivation, or the like.

The methods of the present invention are particularly useful for gene discovery, that is, for identifying, from genomic sequence, regions that function to encode genes, and in a particularly useful embodiment, for identifying regions that function to encode genes not hitherto identified by expression-based or directed cloning and sequencing. In conjunction with verification using the novel single exon microarrays of the present invention, as further described below, the methods herein described become powerful gene discovery tools.

Accordingly, in a preferred embodiment of the present invention, process 25 is used to identify putative coding regions. Two preferred approaches in process 25 for identifying sequence that encodes putative genes are gene prediction and comparative sequence analysis.

Gene prediction can be performed using any of a number of algorithmic methods, embodied in one or more software programs, that identify open reading frames (ORFs) using a variety of heuristics, such as GRAIL, DICTION, and GENEFINDER. Comparative sequence analysis similarly can be performed using any of a variety of known programs that identify regions with lower sequence variability.

As further described in Example 1, below, gene finding software programs yield a range of results. For the newly accessioned human genomic sequence input in 28 Example 1, for example, GRAIL identified the greatest percentage of genomic sequence as putative coding region, 2% of the data analyzed; GENEFINDER was second, calling 1%; and DICTION yielded the least putative coding region, with 0.8% of genomic sequence called as coding region.

Increased reliability can be obtained when consensus is required among several such methods. Although discussed herein particularly with respect to exon calling, consensus among methods will in general increase reliability of predicting other functions as well.

Thus, as indicated by query 26, sequence processing 25, optionally with preprocessing 24, can be repeated with a different method, with consensus among such iterations determined and reported in process 27.

Process 27 compares the several outputs for a given input genomic sequence and identifies consensus among the separately reported results. The consensus itself, as well as the sequence meeting that consensus, is then stored in process 29a, displayed in process 29b, and/or output to process 300 for subsequent identification of a subset thereof suitable for assay.

Multiple levels of consensus can be calculated and reported by process 27. For example, as further described in Example 1, infra, process 27 can report consensus as between all specific pairs of methods of gene prediction, as consensus among any one or more of the pairs of methods of gene prediction, or as among all of the gene prediction algorithms used. Thus, in Example 1, process 27 reported that GRAIL and GENEFINDER programs agreed on 0.7% of genomic sequence, that GRAIL and DICTION agreed on 0.5% of genomic sequence, and that the three programs together agreed on 0.25% of the data analyzed. Put another way, 0.25% of the genomic sequence was identified by all three of the programs as containing putative coding region.

29 Furthermore, consensus can be required among different approaches to identifying a chosen function.

For example, if the function desired to be identified is coding of protein sequence, and a first used approach to exon calling is gene prediction, the process can be repeated on the same input sequence, or subset thereof, with another approach, such as comparative sequence analysis. In such a case, where comparative sequence analysis follows gene prediction, the comparison can be performed not only on genomic nucleic acid sequence, but additionally or alternatively can be performed on the predicted amino acid sequence translated from the ORFs prior identified by the gene prediction approach.

Although shown as an iterative process, the multiple analyses required to achieve consensus can be done in series, in parallel, or some combination thereof.

Predicted functional sequence, optionally representing a consensus among a plurality of methods and approaches for determination thereof, is passed to process 300 for identification of a subset thereof for functional assay.

In the preferred embodiment of the methods of the present invention, wherein the function sought to be identified is protein coding, process 300 is used to identify a subset thereof suitable for experimental verification by physical and/or bioinformatic approaches.

For example, putative ORFs identified in process 200 can be classified, or binned, bioinformatically into putative genes. This binning can be based inter alia upon consideration of the average number of exons/gene in the species chosen for analysis, upon density of exons that have been called on the genomic sequence, and other empirical rules. Thereafter, one or more among the genespecific ORFs can be chosen for subsequent use in gene expression assay.

Where such subsequent gene expression assay uses amplified nucleic acid, considerations such as desired amplicon length, primer synthesis requirements, putative exon length, sequence GC content, existence of possible secondary structure, and the like can be used to identify and select those ORFs that appear most likely successfully to amplify. Where subsequent gene expression assay relies upon nucleic acid hybridization, whether or not using amplified product, further considerations involving hybridization stringency can be applied to identify that subset of sequences that will most readily permit sequencespecific discrimination at a chosen hybridization and wash stringency. One particular such consideration is avoidance of putative exons that span repetitive sequence; such sequence can hybridize spuriously to nonspecific message, reducing specific signal in the hybridization.

For bioinformatic assay, there are fewer constraints on the sequences that can be tested experimentally, and in this latter case therefore process 300 can output the entirety of the input sequence.

The subset of sequences identified by process 300 as suitable for use in assay is then used in process 400 to create the physical and/or informational substrate for experimental verification of the predictions made in process 200, and thereafter to assay those substrates.

As mentioned, the methods of the present invention are particularly useful for identifying potential coding regions within genomic sequence. In a preferred embodiment of process 400, therefore, the expression of the sequences predicted to encode protein is verified. The combination of the predictive and experimental methods provides a powerful gene discovery engine.

Thus, in another aspect, the present invention provides methods and apparatus for verifying the expression of putative genes identified within genomic sequence. In particular, the invention provides a novel method of verifying gene expression in which expression of predicted ORFs is measured and confirmed using a novel type of nucleic acid microarray, the genome-derived single exon nucleic acid microarrays of the present invention.

Putative ORFs as predicted by a consensus of gene calling, particularly gene prediction, algorithms in process 200, and as further identified as suitable by process 300, are amplified from genomic DNA using the polymerase chain reaction (PCR). Although PCR is conveniently used, other amplification approaches can also be used.

Amplification schemes can be designed to capture the entirety of each predicted ORF in an amplicon with minimal additional (that is, intronic or intergenic) sequence. Because ORFs predicted from human genomic sequence using the methods of the present invention differ in length, such an approach results in amplicons of varying length.

However, most predicted ORFs are shorter than 500 bp in length, and although amplicons of at least about 100 or 200 base pairs can be immobilized as probes on nucleic acid microarrays, early experimental results using the methods of the present invention have suggested that longer amplicons, at least about 400 or 500 base pairs, are more effective. Furthermore, certain advantages derive from application to the microarray of amplicons of defined size.

Therefore, amplification schemes can alternatively, and preferably, be designed to amplify regions of defined size, preferably at least about 300, 400 or 500 bp, centered about each predicted ORF. Such an approach results in a population of amplicons of limited 32 size diversity, but that typically contain intronic and/or intergenic nucleic acid in addition to putative ORF.

Conversely, somewhat fewer than 10% of ORFs predicted from human genomic sequence according to the methods of the present invention exceed 500 bp in length. Portions of such extended ORFs, preferably at least about 300, 400 or 500 bp in length, can be amplified. However, it has been discovered that the percentage success at amplifying pieces of such ORFs is low, and that such putative exons are more effectively amplified when larger fragments, at least about 1000 or 1500 bp, and even as large as 2000 bp are amplified.

The putative ORFs selected in process 300 are thus input into one or more primer design programs, such as PRIMER3 (available online for use at http://www-genome.wi.mit.edu/cgibin/primer/ with a goal of amplifying at least about 500 base pairs of genomic sequence centered within or about ORFs predicted to be no more than about 500 bp, or at least about 1000 1500 bp of genomic sequence for ORFs predicted to exceed 500 bp in length, and the primers synthesized by standard techniques. Primers with the requisite sequences can be purchased commercially or synthesized by standard techniques.

Conveniently, a first predetermined sequence can be added commonly to the ORF-specific 51 primer and a second, typically different, predetermined sequence commonly added to each 31 ORF-unique primer. This serves to immortalize the amplicon, that is, serves to permit further amplification of any amplicon using a single set of primers complementary respectively to the common 51 and common 31 sequence elements. The presence of these "universal" priming sequences further facilitates later sequence verification, providing a sequence common to all amplicons at which to prime sequencing reactions. The 33 common 51 and 31 sequences further serve to add a cloning site should any of the ORFs warrant further study.

Such predetermined sequence is usefully at least about 10, 12 or 15 nt in length, and usually does not exceed about 25 nt in length. The "universal,, priming sequences used in the examples presented infra were each 16 nt long.

The genomic DNA to be used as substrate f or amplification will come from the eukaryotic species from which the genomic sequence data had originally been obtained, or a closely related species, and can conveniently be prepared by well known techniques from somatic or germline tissue or cultured cells of the organism. See, e.g., Short Protocols in Molecular Biolog: A Compendium of Methods from Current Protocols in Molecular Biology, Ausubel et al. (eds. ), 4 th edition (April 1999), John Wiley & Sons (ISBN: 047132938X) and Maniatis et al., Molecular Cloning: A Laboratory Manual, 2 nd edition (December 1989), Cold Spring Harbor Laboratory Press (ISBN: 0879693096). Many such prepared genomic DNAs are available commercially, with the human genomic DNAs additionally having certification of donor informed consent. Although the intronic and intergenic material flanking putative coding

regions in the amplicons could potentially interfere with hybridizations during microarray experiments, we have found, surprisingly, that differential expression ratios are not significantly affected. Rather, the predominant effect of exon size is to alter the absolute signal intensity, rather than its ratio. Equally surprising, the art had suggested that single exon probes would not provide sufficient signal intensity for high stringency hybridization analyses; we find that such probes not only provide adequate signal, but have substantial 34 advantages, as herein described.

After partial purification, as by size exclusion spin column, with or without confirmation as to amplicon quality as by gel electrophoresis, each amplicon (single 5 exon probe) is disposed in an array upon a support substrate.

Methods for creating microarrays by deposition and fixation of nucleic acids onto support substrates are well known in the art (Reviewed by Schena et al., see above).

Typically, the support substrate will be glass, although other materials, such as amorphous or crystalline silicon or plastics. Such plastics include polymethylacrylic, polyethylene, polypropylene, polyacrylate, polymethylmethacrylate, polyvinylchloride, polytetrafluoroethylene, polystyrene, polycarbonate, polyacetal, polysulfone, celluloseacetate, cellulosenitrate, nitrocellulose, or mixtures thereof, can also be used. Typically, the support will be rectangular, although other shapes, particularly circular disks and even spheres, present certain advantages. Particularly advantageous alternatives to glass slides as support substrates for array of nucleic acids are optical discs, as described in WO 98/12559.

The amplified nucleic acids can be attached covalently to a surface of the support substrate or, more typically, applied to a derivatized surface in a chaotropic agent that facilitates denaturation and adherence by presumed noncovalent interactions, or some combination thereof.

Robotic spotting devices useful for arraying nucleic acids on support substrates can be constructed using public domain specifications (The MGuide, version 2.0, http: / /cmgm. stanf ord. edu/pbrown/mguide/ index. html), or can conveniently be purchased from commercial sources (MicroArray GenII Spotter and MicroArray GenIII Spotter, Molecular Dynamics, Inc., Sunnyvale, CA). Spotting can also be effected by printing methods, including those using 5 ink jet technology.

As is well known in the art. microarrays typically also contain immobilized control nucleic acids. For controls useful in providing measurements of background signal for the genome-derived single exon microarrays of the present invention, a plurality of E. coli genes can readily be used. As further described in Example 1, 16 or 32 E. coli genes suffice to provide a robust measure of background noise in such microarrays.

As is well known in the art. the amplified product disposed in arrays on a support substrate to create a nucleic acid microarray can consist entirely of natural nucleotides linked by phosphodiester bonds, or alternatively can include either nonnative nucleotides, alternative internucleotide linkages, or both, so long as complementary binding can be obtained in the hybridization. If enzymatic amplification is used to produce the immobilized probes, the amplifying enzyme will impose certain further constraints upon the types of nucleic acid analogs that can be generated.

Although particularly described herein as using high density microarrays constructed on planar substrates, the methods of the present invention for confirming the expression of ORFs predicted from genomic sequence can use any of the known types of microarrays, as herein defined, including lower density planar arrays, and microarrays on nonplanar, nonunitary, distributed substrates.

For example, gene expression can be confirmed using hybridization to lower density arrays, such as those constructed on membranes, such as nitrocellulose, nylon, 36 and positively-charged derivatized nylon membranes.

Further, gene expression can also be confirmed using nonplanar, bead-based microarrays such as are described in Brenner et al., P-roc. Natl. Acad. Sci. USA 97(4):166501670 (2000); U. S. Patent No. 6, 057, 107; and U. S. Patent No.

5,736,330. In theory, a packed collection of such beads provides in aggregate a higher density of nucleic acid probe than can be achieved with spotting or lithography techniques on a single planar substrate.

Planar microarrays on solid substrates, however, provide certain useful advantages, including high throughput and compatibility with existing readers. For example, each standard microscope slide can include at least 1000, typically at least 2000, preferably 5000 and upto 10,000 - 50,000 or more nucleic acid probes of discrete sequence. The number of sequences deposited will depend on their required application.

Each putative gene can be represented in the array by a single predicted ORF. Alternatively, genes can be represented by more than one predicted ORF. For purposes of measuring differential splicing, more than one predicted ORF will be provided for a putative gene. And as is well known in the art, each probe of defined sequence, representing a single predicted ORF, can be deposited in a plurality of locations on a single microarray to provide redundancy of signal.

The genome-derived single exon microarrays described above differ in several fundamental and advantageous ways from microarrays presently used in the gene expression art, including (1) those created by deposition of mRNA- derived nucleic acids, (2) those created by in situ synthesis of oligonucleotide probes, and (3) those constructed from yeast genomic DNA.

Most nucleic acid microarrays that are in use for 37 study of eukaryotic gene expression have as immobilized probes nucleic acids that are derived - either directly or indirectly - from expressed message. As discussed above, it is common, for example, for such microarrays to be derived from cDNA/EST libraries, either from those previously described in the literature, see Lennon et al., or from the de novo construction of "problem specific,, libraries targeted at a particular biological question, R.S. Thomas et al., Cancer Res. (in press). Such microarrays are herein collectively denominated 1IEST microarraysll.

Such EST microarrays, by definition can measure expression only of those genes found in EST libraries, shown herein to represent only a fraction of expressed genes. Furthermore, such libraries - and thus microarrays based thereupon - are biased by the tissue or cell type of message origin, by the expression levels of the respective genes within the tissues, and by the ability of the message successfully to have been reverse-transcribed and cloned.

Thus, as further discussed in Example 1, the methods of the present invention enable sequences that do not appear in EST or other expression databases to be determined subsequently arrayed for expression measurements could not, therefore, have been represented as probes on an EST microarray. And as further demonstrated in the examples, infra, the remaining population of genes identified from genomic sequence by the methods of the present invention - that is, the one third of sequences that had previously been accessioned in EST or other expression databases - are biased toward genes with higher expression levels.

Representation of a message in an EST and/or cDNA library depends upon the successful reverse transcription, optionally but typically with subsequent successful 38 cloning, of the message. This introduces substantial bias into the population of probes available for arraying in EST microarrays.

In contrast, neither reverse transcription nor cloning is required to produce the probes arrayed on the genomederived single exon microarrays of the present invention. And although the ultimate deposition of a probe on the genome-derived single exon microarray of the present invention depends upon a successful amplification from genomic material, a priori knowledge of the sequence of the desired amplicon affords greater opportunity to recover any given probe sequence recalcitrant to amplification than is afforded by the requirement for successful reverse transcription and cloning of unknown message in EST approaches.

Thus, the genome-derived single exon microarrays of the present invention present a far greater diversity of probes for measuring gene expression, with far less bias, than do EST microarrays presently used in the art.

As a further consequence of their ultimate origin from expressed message, the probes in EST microarrays often contain poly-A (or complementary polyT) stretches derived from the poly-A tail of mature mRNA. These homopolymeric stretches contribute to cross-hybridization, that is, to a spurious signal occasioned by hybridization to the homopolymeric tail of a labeled cDNA that lacks sequence homology to the genespecific portion of the probe.

In contrast, the probes arrayed in the genomederived single exon microarrays of the present invention lack homopolymeric stretches derived from message polyadenylation, and thus can provide more specific signal. Typically, at least about 50, 60 or 75% of the probes on the genomederived single exon microarrays of the present invention lack homopolymeric regions consisting of A or T, 39 where a homopolymeric region is defined for purposes herein as stretches of 25 or more, typically 30 or more, identical nucleotides.

A further distinction, which also affects the specificity of hybridization, is occasioned by the typical derivation of EST microarray probes from cloned material. Because much of the probe material disposed as probes on EST microarrays is excised or amplified from plasmid, phage, or phagemid vectors, EST microarrays typically include a fair amount of vector sequence, more so when the probes are amplified, rather than excised, from the vector.

In contrast, the vast majority of probes in the genome-derived single exon microarrays of the present invention contain no prokaryotic or bacteriophage vector sequence, having been amplified directly or indirectly from genomic DNA. Typically, therefore, at least about 50, 60, 70 or 80% or more of individual exon-including probes disposed on a genome-derived single exon microarray of the present invention lack vector sequence, and particularly lack sequences drawn from plasmids and bacteriophage. Preferably, at least about 85, 90 or more than 90% of exonincluding probes in the genome- derived single exon microarray of the present invention lack vector sequence. With attention to removal of vector sequences through preprocessing 24, percentages of vector-free exon-including probes can be as high as 95 - 99%. The substantial absence of vector sequence from the genome-derived single exon microarrays of the present invention results in greater specificity during hybridization, since spurious cross- hybridization to a probe vector sequence is reduced.

As a further consequence of excision or amplification of probes from vectors in construction of EST microarrays, the probes arrayed thereon often contain artificial sequence, derived from vector polylinker multiple cloning sites, at both 51 and 31 ends. The probes disposed upon the genome-derived single exon microarrays need have no such artificial sequence appended thereto.

As mentioned above, however, the ORF-specific primers used to amplify putative ORFs can include artificial sequences, typically 51 to the ORFspecific primer sequence, useful for "universal,, (that is, independent of ORF sequence) priming of subsequent amplification or sequencing reactions. When such "universal" 51 and/or 31 priming sequences are appended to the amplification primers, the probes disposed upon the genome-derived single exon microarray will include artificial sequence similar to that found in EST microarrays. However, the genome-derived single exon microarray of the present invention can be made without such sequences, and if so constructed, presents an even smaller amount of nonspecific sequence that would contribute to nonspecific hybridization.

Yet another consequence of typical use of cloned material as probes in EST microarrays is that such microarrays contain probes that result from cloning artifacts, such as chimeric molecules containing coding region of two separate genes. Derived from genomic material, typically not thereafter cloned, the probes of the genome-derived single exon microarrays of the present invention lack such cloning artifacts, and thus provide greater specificity of signal in gene expression measurements.

A further consequence of the cloned origin of probes on many EST microarrays is that the individual probes often have disparate sizes, which can cause the optimal hybridization stringency to vary among probes on a single microarray. In contrast, as discussed above, the probes arrayed on the genome-derived single exon 41 microarrays of the present invention can readily be designed to have a narrow distribution in sizes, with the range of probe sizes no greater than about 10% of the average size, typically no greater than about 5% of the 5 average probe size.

Because of their origin from fully- or partiallyspliced message, probes disposed upon EST arrays will often include multiple exons. The percentage of such exonspanning probes in an EST microarray can be calculated, on average, based upon the predicted number of exons/gene for the given species and the average length of the immobilized probes. For human genes, the near-complete sequence of human chromosome 22, Dunham et al., Nature 402(676l):48995 (1999), predicts that human genes average 5.5 exons/gene.

Even with probes of 200 - 500 bp, the vast majority of human EST microarray probes include more than one exon.

In contrast, by virtue of their origin from algorithmically identified ORFs in genomic sequence, the probes in the genome-derived single exon microarrays of the present invention can consist of individual exons. Thus, in contrast to EST microarrays, at least about 50, 60, 70, 75, 80, 85, 95 or 99% of probes deposited in the genomederived microarray of the present invention consist of, or include, no more than one predicted ORF.

This provides the ability, not readily achieved using EST microarrays, to use the genome-derived single exon microarrays of the present invention to measure tissue-specific expression of individual exons, which in turn allows differential splicing events to be detected and characterized, and in particular, allows the correlation of differential splicing to tissue-specific expression patterns.

Furthermore, the exons that are represented in EST microarrays are of ten biased toward the 31 or 51 end of 42 their respective genes, since sequencing strategies used for EST identification are so biased. In contrast, no such 31 or 51 bias necessarily inheres in the selection of exons for disposition on the genome-derived single exon microarrays of the present invention.

Conversely, the probes provided on the genomederived single exon microarrays of the present invention typically, but need not necessarily, include intronic and/or intergenic sequence that is absent from EST microarrays, which are derived from mature niRNA. Typically, at least about 50, 60, 70, 80 or 90% of the exon-including probes on the genomederived single exon microarrays of the present invention include sequence drawn from noncoding regions. As discussed above, the additional presence of noncoding region does not significantly interfere with measurement of gene expression, and provides the additional opportunity to assay prespliced RNA, and thus measure such phenomena such as nuclear export control.

The genome-derived single exon microarrays of the present invention are also quite different from in situ synthesis microarrays, where probe size is severely constrained by inadequacies in the photolithographic synthesis process.

Typically, probes arrayed on in situ synthesis microarrays are limited to a maximum of about 25 bp. As a well known consequence, hybridization to such chips must be performed at low stringency. In order, therefore, to achieve unambiguous sequence-specific hybridization results, the in situ synthesis microarray requires substantial redundancy, with concomitant programmed arraying for each probe of probe analogues with altered (i.e., mismatched) sequence.

In contrast, the longer probe length of the genome-derived single exon microarrays of the present 43 invention allows much higher stringency hybridization and wash. Typically, therefore, exon-including probes on the genome-derived single exon microarrays of the present invention average at least about 100, 200, 300, 400 or 500 bp in length. By obviating the need for substantial probe redundancy, this approach permits a higher density of probes for discrete exons or genes to be arrayed on the microarrays of the present invention than can be achieved for in situ synthesis microarrays.

A further distinction is that the probes in in situ synthesis microarrays typically are covalently linked to the substrate surface. In contrast, the probes disposed on the genome-derived microarray of the present invention typically are, but need not necessarily be, bound noncovalently to the substrate.

Furthermore, the short probe size on in situ microarrays causes large percentage differences in the melting temperature of probes hybridized to their complementary target sequence, and thus causes large percentage differences in the theoretically optimum stringency across the array as a whole.

In contrast, the larger probe size in the microarrays of the present invention create lower percentage differences in melting temperature across the range of arrayed probes.

A further significant advantage of the microarrays of the present invention over in situ synthesized arrays is that the quality of each individual probe can be confirmed before deposition. In contrast, the quality of probes cannot be assessed on a probe-by-probe basis for the in situ synthesized microarrays presently being used.

The genome-derived single exon microarrays of the present invention are also distinguished over, and present 44 substantial benefits over, the genome-derived microarrays from lower eukaryotes such as yeast. Lashkari et al., Proc. Natl. Acad. Sci. USA 94:13057-13062 (1997).

Only about 220 - 250 of the 6100 or so nuclear genes in Saccharomyces cerevisiae - that is, only about 4 - 5% - have standard, spliceosomal, introns, Lopez et al., Nucl. Acids Res. 28:85-86 (2000); Spingola et al., RNA 5 (2):221-34 (1999). Furthermore, the entire yeast genome has already been sequenced. These two facts permit the ready amplification and disposition of singleORF amplicons on such microarray without the requirement for antecedent use of gene prediction and/or comparative sequence analyses.

Thus, a significant aspect of the present invention is the ability to identify and to confirm expression of predicted coding regions in genomic sequence drawn from eukaryotic organisms that have a higher percentage of genes having introns than do yeast such as Saccharomyces cerevisiae, particularly in genomic sequence drawn from eukaryotes in which at least about 10, 20 or 50% of protein- encoding genes have introns. In preferred embodiments, the methods and apparatus of the present invention are used to identify and confirm expression of novel genes from genomic sequence of eukaryotes in which the average number of introns per gene is at least about one, two or three or more.

After the physical substrate is prepared, experimental verification of predicted function is performed.

In a preferred embodiment of the present invention, where the function sought to be identified in genomic sequence is protein coding, experimental verification is performed by measuring expression of the putative ORFs, typically through nucleic acid hybridization experiments, and in particularly preferred embodiments, through hybridization to genome-derived single exon microarrays prepared as above- described.

Expression is conveniently measured and expressed for each probe in the microarray as a ratio of the expression measured concurrently in a plurality of mRNA sources, according to techniques well known in the microarray art, Reviewed in Schena et al., and as further described in Example 2, below. The mRNA source for the reference against which specific expression is measured can be drawn from a homogeneous mRNA source, such as a single cultured cell-type, or alternatively can be heterogeneous, as from a pool of mRNA derived from multiple tissues and/or cell types, as further described in Example 2, infra.

mRNA can be prepared by standard techniques, see Ausubel et al. and Maniatis et al., or purchased commercially. The mRNA is then typically reversetranscribed in the presence of labeled nucleotides: the index source (that in which expression is desired to be measured) is reverse transcribed in the presence of nucleotides labeled with a first label, typically a fluorophore (fluorochrome; fluor; fluorescent dye); the reference source is reverse transcribed in the presence of a second label, typically a fluorophore, typically fluorometrically-distinguishable from the first label. As further described in Example 2, infra, Cy3 and CyS dyes prove particularly useful in these methods. After partial purification of the index and reference targets, hybridization to the probe array is conducted according to standard techniques, typically under a coverslip.

After wash, microarrays are conveniently scanned using a commercial microarray scanning device, such as a Gen3 Scanner (Molecular Dynamics, Sunnyvale, CA). Data on expression is then passed, with or without interim storage, 46 to process 500, where the results for each probe are related to the original sequence.

Often, hybridization of target material to the genome-derived single exon microarray will identify certain of the probes thereon as of particular interest. Thus, it is often desirable that the user be able readily to obtain sufficient quantities of an individual probe, either for subsequent arrayed deposition upon an additional support substrate, often as part of a microarray having a plurality of probes so identified, or alternatively or additionally as a solitary solid-phase or solution-phase probe, for further use.

Thus, in another aspect, the present invention provides compositions and kits for the ready production of nucleic acids identical in sequence to, or substantially identical in sequence to, probes on the genome-derived single exon microarrays of the present invention.

In this aspect, a small quantity of each probe is disposed, typically without attachment to substrate, in a spatially- addressable ordered set, typically one per well of a microtiter dish. Although a 96 well microtiter plate can be used, greater efficiency is obtained using higher density arrays, such as are provided by microtiter plates having 384, 864, 1536, 3456, 6144, or 9600 wells, and although microtiter plates having physical depressions (wells) are conveniently used, any device that permits addressable withdrawal of reagent from fluidlynoncommunicating areas can be used.

In this aspect of the invention, therefore, a fluidly noncommunicating addressable ordered set of individual probes, corresponding to those on a genomederived single exon microarray, is provided, with each probe in sufficient quantity to permit amplification, such as by PCR. As earlier mentioned, the ORF-specific 47 51 primers used for genomic amplification can have a first common sequence added thereto, and the ORF-specific 31 primers used for genomic amplification can have a second, different, common sequence added thereto, thus permitting, in this preferred embodiment, the use of a single set of 5 1 and 31 primers to amplify any one of the probes from the amplifiable ordered set.

Each discrete amplifiable probe can also be packaged with amplification primers, solutes, buffers, etc., and can be provided in dry (e.g., lyophilized) form or wet, in the latter case typically with addition of agents that retard evaporation.

In another aspect of the present invention, a genome-derived single-exon microarray is packaged together with such an ordered set of amplifiable probes corresponding to the probes, or one or more subsets of probes, thereon. In alternative embodiments, the ordered set of amplifiable probes is packaged separately from the genome-derived single exon microarray.

In some embodiments, the microarray and/or ordered probe set are further packaged with recordable media that provide probe identification and addressing information, and that can additionally contain annotation information, such as gene expression data. Such recordable media can be packaged with the microarray, with the ordered probe set, or with both.

If the microarray is constructed on a substrate that incorporates recordable media, such as is described in international patent application no. WO 98/12559, then separate packaging of the genome-derived single exon microarray and the bioinformatic information is not required.

The amount of amplifiable probe material should be sufficient to permit at least one amplification 48 sufficient for subsequent hybridization assay.

Although the use of high density genome-derived microarrays on solid planar substrates is presently a preferred approach for the physical confirmation and characterization of the expression of sequences predicted to encode protein, other types of microarrays (as herein defined) can also be used.

Furthermore, as earlier mentioned, experimental verification of the function predicted from genomic sequence in process 200 can be bioinformatic, rather than, or additional to, physical verification.

For example, where the function desired to be identified is protein coding, the predicted ORFs can be compared bioinformatically to sequences known or suspected of being expressed.

Thus, the sequences output from process 300 (or process 200), can be used to query expression databases, such as EST databases, SNP ("single nucleotide polymorphism11) databases, known cDNA and mRNA sequences, SAGE ("serial analysis of gene expression,') databases, and more generalized sequence databases that allow query for expressed sequences. Such query can be done by any sequence query algorithm, such as BLAST (,, basic local alignment search tool"). The results of such query - including information on identical sequences and information on nonidentical sequences that have diffuse or focal regions of sequence homology to the query sequence can then be passed directly to process 500, or used to inform analyses subsequently undertaken in process 200, process 300, or process 400.

Experimental data, whether obtained by physical or bioinformatic assay in process 400, is passed to process 500 where it is usefully related to the sequence data itself, a process colloquially termed "annotation". Such 49 annotation can be done using any technique that usefully relates the functional information to the sequence, as, for example, by incorporating the functional data into the record itself, by linking records in a hierarchical or relational database. by linking to external databases, or by a combination thereof. Such database techniques are well within the skill in the art.

The annotated sequence data can be stored locally, uploaded to genomic sequence database 100, and/or displayed 800.

The methods and apparatus of the present invention rapidly produce functional information from genomic sequence. Coupled with the escalating pace at which sequence now accumulates, the rapid pace of sequence annotation produces a need for methods of displaying the information in meaningful ways.

FIG. 3 shows visual display 80 presenting a single genomic sequence annotated according to the present invention. Because of its nominal resemblance to artistic works of Piet Mondrian, visual display 80 is alternatively described herein as a 11Mondrianll. Each of the visual elements of display 80 is aligned with respect to the

genomic sequence being annotated (hereinafter, the "annotated sequence"). Given the number of nucleotides typically represented in an annotated sequence, representation of individual nucleotides would rarely be readable in hard copy output of display 80. Typically, therefore, the annotated sequence is schematized as rectangle 89, extending from the left border of display 80 to its right border. By convention herein, the left border of rectangle 89 represents the first nucleotide of the sequence and the right border of rectangle 89 represents the last nucleotide of the sequence.

As further discussed below, however, the Mondrian visual display of annotated sequence can serve as a convenient graphical user interface for computerized representation, analysis, and query of information stored electronically. For such use, the individual nucleotides can conveniently be linked to the X axis coordinate of rectangle 89. This permits the annotated sequence at any point within rectangle 89 readily to be viewed, either automatically - for example, by time-delayed appearance of a small overlaid window upon movement of a cursor or other pointer over rectangle 89 - or through user intervention, as by clicking a mouse or other pointing device at a point in rectangle 89.

Visual display 80 is generated after user specification of the genomic sequence to be displayed. Such specification can consist of or include an accession number for a single clone (e.g., a single BAC accessioned into GenBank), wherein the starting and stopping nucleotides are thus absolutely identified, or alternatively can consist of or include an anchor or fulcrum point about which a chosen range of sequence is anchored, thus providing relative endpoints for the sequence to be displayed. For example, the user can anchor such a range about a given chromosomal map location, gene name, or even a sequence returned by query for similarity or identity to an input query sequence. When visual display 80 is used as a graphical user interface to computerized data, additional control over the first and last displayed nucleotide will typically be dynamically selectable, as by use of standard zooming and/or selection tools.

Field 81 of visual display 80 is used to present the output from process 200. that is, to present the bioinformatic prediction of those sequences having the

51 desired function within the genomic sequence. Functional sequences are typically indicated by at least one rectangle 83 (83a, 83b, 83c), the left and right borders of which respectively indicate, by their X-axis coordinates, the starting and ending nucleotides of the region predicted to have function.

Where a single bioinformatic method or approach identifies a plurality of regions having the desired function, a plurality of rectangles 83 is disposed horizontally in field 81. Where multiple methods and/or approaches are used to identify function, each such method and/or approach can be represented by its own series of horizontally disposed rectangles 83, each such horizontally disposed series of rectangles offset vertically from those representing the results of the other methods and approaches.

Thus, rectangles 83a in FIG. 3 represent the functional predictions of a first method of a first approach for predicting function, rectangles 83b represent the functional predictions of a second method and/or second approach for predicting that function, and rectangles 83c represent the predictions of a third method and/or approach.

Where the function desired to be identified is protein coding, field 81 is used to present the bioinformatic prediction of sequences encoding protein. For example, rectangles 83a can represent the results from GRAIL or GRAIL II, rectangles 83b can represent the results from GENEFINDER, and rectangles 83c can represent the results from DICTION.

Optionally, and preferably, rectangles 83 collectively representing predictions of a single method and/or approach are identically colored and/or textured, and are distinguishable from the color and/or texture used 52 for a different method and/or approach.

Alternatively, or in addition, the color, hue, density, or texture of rectangles 83 can be used further to report a measure of the bioinformatic reliability of the prediction. For example, many gene prediction programs will report a measure of the reliability of prediction. Thus, increasing degrees of such reliability can be indicated, e.g., by increasing density of shading. Where display 80 is used as a graphical user interface, such measures of reliability, and indeed all other results output by the program, can additionally or alternatively be made accessible through linkage from individual rectangles 83, as by time-delayed window ("tool tip,' window), or by pointer (e.g., mouse)-activated link.

As earlier described, increased predictive reliability can be achieved by requiring consensus among methods and/or approaches to determining function. Thus, field 81 can include a horizontal series of rectangles 83 that indicate one or more degrees of consensus in predictions of function.

Although FIG. 3 shows three series of horizontally disposed rectangles in field 81, display 80 can include as few as one such series of rectangles and as many as can discriminably be displayed, depending upon the number of methods and/or approaches used to predict a given function.

Furthermore, field 81 can be used to show predictions of a plurality of different functions. However, the increased visual complexity occasioned by such display makes more useful the ability of the user to select a single function for display. When display 80 is used as a graphical user interface for computer query and analysis, such function can usefully be indicated and userselectable, as by a series of graphical buttons or tabs 53 (not shown in FIG. 3).

Rectangle 89 is shown in FIG. 3 as including interposed rectangle 84. Rectangle 84 represents the portion of annotated sequence for which predicted functional information has been assayed physically, with the starting and ending nucleotides of the assayed material indicated by the X axis coordinates of the left and right borders of rectangle 84. Rectangle 85, with optional inclusive circles 86 (86a, 86b, and 86c) displays the results of such physical assay.

Although a single rectangle 84 is shown in FIG.

3, physical assay is not limited to just one region of annotated genomic sequence. It is expected that an increasing percentage of regions predicted to have function by process 200 will be assayed physically, and that display will accordingly, for any given genomic sequence, have an increasing number of rectangles 84 and 85, representing an increased density of sequence annotation.

Where the function desired to be identified is protein coding, rectangle 84 identifies the sequence of the probe used to measure expression. In embodiments of the present invention where expression is measured using genome-derived single exon microarrays, rectangle 84 identifies the sequence included within the probe immobilized on the support surface of the microarray. As noted supra, such probe will often include a small amount of additional, synthetic, material incorporated during amplification and designed to permit reamplification of the probe, which sequence is typically not shown in display 80.

Rectangle 87 is used to present the results of bioinformatic assay of the genomic sequence. For example, where the function desired to be identified is protein coding, process 400 can include bioinformatic query of expression databases with the sequences predicted in 54 process 200 to encode exons. And as earlier discussed, because bioinformatic assay presents fewer constraints than does physical assay, often the entire output of process 200 can be used for such assay, without further subsetting thereof by process 300. Therefore, rectangle 87 typically need not have separate indicators therein of regions submitted for bioinformatic assay; that is, rectangle 87 typically need not have regions therein analogous to rectangles 84 within rectangle 89.

* Rectangle 87 as shown in FIG. 3 includes smaller rectangles 880 and 88. Rectangles 880 indicate regions that returned a positive result in the bioinformatic assay, with rectangles 88 representing regions that did not return such positive results. Where the function desired to be predicted and displayed is protein coding, rectangles 880 indicate regions of the predicted exons that identify sequence with significant similarity in expression databases, such as EST, SNP, SAGE databases, with rectangles 88 indicating genes novel over those identified in existing expression data bases.

Rectangles 880 can further indicate, through color, shading, texture, or the like, additional information obtained from bioinformatic assay.

For example, where the function assayed and displayed is protein coding, the degree of shading of rectangles 880 can be used to represent the degree of sequence similarity found upon query of expression databases. The number of levels of discrimination can be as few as two (identity, and similarity, where similarity has a user-selectable lower threshold). Alternatively, as many different levels of discrimination can be indicated as can visually be discriminated.

Where display 80 is used as a graphical user interface, rectangles 880 can additionally provide links directly to the sequences identified by the query of expression databases, and/or statistical summaries thereof. As with each of the precedingly- discussed uses of display 80 as a graphical user interface, it should be understood that the information accessed via display 80 need not be resident on the computer presenting such display, which often will be serving as a client, with the linked information resident on one or more remotely located servers.

Rectangle 85 displays the results of physical assay of the sequence delimited by its left and right borders.

Rectangle 85 can consist of a single rectangle, thus indicating a single assay, or alternatively, and increasingly typically, will consist of a series of rectangles (85a, 85b, 85c) indicating separate physical assays of the same sequence.

Where the function assayed is gene expression, and where gene expression is assayed as herein described using simultaneous two-color fluorescent detection of hybridization to genome-derived single exon microarrays, individual rectangles 85 can be colored to indicate the degree of expression relative to control. Conveniently, shades of green can be used to depict expression in the sample over control values, and shades of red used to depict expression less than control, corresponding to the spectra of the Cy3 and CyS dyes conventionally used for respective labeling thereof. Additional functional information can be provided in the form of circles 86 (86a, 86b, 86c), where the diameter of the circle can be used to indicate expression intensity. As discussed infra, such relative expression (expression ratios) and absolute expression (signal intensity) can be expressed using normalized values.

56 Where display 80 is used as a graphical user interface, rectangle 85 can be used as a link to further information about the assay. For example, where the assay is one for gene expression, each rectangle 85 can be used 5 to link to information about the source of the hybridized mRNA, the identity of the control, raw or processed data from the microarray scan, or the like.

FIG. 4 is rendition of display 80 representing gene prediction and gene expression for a hypothetical BAC, showing conventions used in the Examples presented infra. BAC sequence PChip seq.") 89 is presented, with the physically assayed region thereof (corresponding to rectangle 84 in FIG. 3) shown in white. Algorithmic gene predictions are shown in field 81, with predictions by

GRAIL shown, predictions by GENEFINDER, and predictions by DICTION shown. Within rectangle 87, regions of sequence that, when used to query expression databases, return identical or similar sequences (11EST hit") are shown as white rectangles (corresponding to rectangles 880 in FIG.

3), gray indicates low homology, and black indicates unknowns (where black and gray would correspond to rectangles 88 in FIG. 3).

Although FIGS. 3 and 4 show a single stretch of sequence, uninterrupted from left to right, longer sequences are usefully represented by vertical stacking of such individual Mondrians, as shown in FIGS. 9 and 10.

Single Exon Probes Useful For Measuring Gene Expression The methods and apparatus of the present invention rapidly produce functional information from genomic sequence. Where the function to be identified is protein coding, the methods and apparatus of the present invention rapidly identify and confirm the expression of 57 portions of genomic sequence that function to encode protein. As a direct result, the methods and apparatus of the present invention rapidly yield large numbers of single-exon nucleic acid probes, the majority from previously unknown genes, each of which is useful for measuring and/or surveying expression of a specific gene in one or more tissues or cell types.

It is, therefore, another aspect of the present invention to provide genome-derived single exon nucleic acid probes useful for gene expression analysis, and particularly for gene expression analysis by microarray.

Using the methods and genome-derived single-exon microarrays of the present invention, we have for example readily identified a large number of unique ORFs from human genomic sequence. Using single exon probes that encompass these ORFs, we have demonstrated, through microarray hybridization analysis, the expression of 5,081 of these ORFs in heart.

As would immediately be appreciated by one of skill in the art, each single exon probe having demonstrable expression in heart is currently available for use in measuring the level of its ORF1s expression in heart. The utility is specific to the probe; at sufficiently high hybridization stringency. which stringencies are well known in the art - see Ausubel et al. and Maniatis et al. - each probe reports the level of expression of message specifically containing that ORF.

It should be appreciated, however, that the probes of the present invention, for which expression in the heart has been demonstrated are useful for both measurement in the heart and for survey of expression in other tissues.

Significant among such advantages is the presence of probes for novel genes.

58 As mentioned above and further detailed in Examples 1 and 2, the methods described enable ORFs which are not present in existing expression databases to be identified. And the fewer the number of tissues in which 5 the ORF can be shown to be expressed, the more likely the ORF will prove to be part of a novel gene: as further discussed in Example 2, ORFs whose expression was measurable in only a single of the tested tissues were represented in existing expression databases at a rate of only 11%, whereas 36% of ORFs whose expression was measurable in 9 tissues were present in existing expression databases, and fully 45% of those ORFs expressed in all ten tested tissues were present in existing expressed sequence databases. Either as tools for measuring gene expression or tools for surveying gene expression, the genome-derived single exon probes of the present invention have significant advantages over the cDNA or EST-based probes that are currently available for achieving these utilities. 20 The genomederived single exon probes of the present invention are useful in constructing genome-derived single exon microarrays; the genome-derived single exon microarrays, in turn, are useful devices for measuring and for surveying gene expression in the human. Gene expression analysis using microarrays conventionally using microarrays having probes derived from expressed message - is well-established as useful in the biological research arts (see Lockhart et al. Nature 405, 827-836). Microarrays have been used to determine gene expression profiles in cells in response to drug treatment (see, for example, Kaminski et al., "Global Analysis of Gene Expression in Pulmonary Fibrosis Reveals Distinct Programs Regulating Lung Inflammation and Fibrosis," Proc.

59 Natl. Acad. Sci. USA 97 (4):1778-83 (2000); Bartosiewicz et al., "Development of a Toxicological Gene Array and Quantitative Assessment of This Technology, 11 Arch. Blochem. Blophys. 376 (1):6673 (2000)), viral infection (see for example, Geiss et al., "Large-scale Monitoring of Host Cell Gene Expression During HIV-1 Infection Using eDNA Microarrays, 11 Virology 266 (1):8-16 (2000)) and during cell processes such as differentiation, senescence and apoptosis (see, for example, Shelton et al., I'Microarray Analysis of Replicative Senescence, 11 Curr. Biol. 9 (17):939-45 (1999); Voehringer et al., "Gene Microarray Identification of Redox and Mitochondrial Elements That Control Resistance or Sensitivity to Apoptosis,11 Proc. Natl. Acad. Sci. USA 97(6):2680-5 (2000H.

Microarrays have also been used to determine abnormal gene expression in diseased tissues (see, for example, Alon et al., "Broad Patterns of Gene Expression Revealed by Clustering Analysis of Tumor and Normal Colon Tissues Probed by Oligonucleotide Arrays,11 Proc. Natl.

Acad. Sci. USA 96 (12):674550 (1999); Perou et: al., "Distinctive Gene Expression Patterns in Human Mammary Epithelial Cells and Breast Cancers, Proc. Natl. Acad. Scl. USA 96 (16):9212-7 (1999); Wang et al., "Identification of Genes Differentially Over-expressed in Lung Squamous Cell Carcinoma Using Combination of cDNA Subtraction and Microarray Analysis, 11 Oncogene 19 (12):1519-28 (2000) Whitney et al., "Analysis of Gene Expression in Multiple Sclerosis Lesions Using cDNA Microarrays, 11 Ann. Neurol. 46 (3):425-8 (1999)), in drug discovery screens (see, for example, Scherf et al., "A Gene Expression Database for the Molecular Pharmacology of Cancer, 11 Nat. Genet. 24 (3):23 6 -44 (2000)) and in diagnosis to determine appropriate treatment strategies (see, for example, Sgroi et al., "In vivo Gene Expression Profile Analysis of Human Breast Cancer Progression, 11 Cancer -Res. 59 (22):5656-61 (1999) In mi croarraybased gene expression screens of pharmacological drug candidates upon cells, each probe provides specific useful data. In particular, it should be appreciated that even those probes that show no change in expression are as informative as those that do change, serving, in essence, as negative controls.

For example, where gene expression analysis is used to assess toxicity of chemical agents on cells, the failure of the agent to change a gene's expression level is evidence that the drug likely does not affect the pathway of which the gene' s expressed protein is a part. Analogously, where gene expression analysis is used to assess side effects of pharmacological agents - whether in lead compound discovery or in subsequent screening of lead compound derivatives - the inability of the agent to alter a gene's expression level is evidence that the drug does not affect the pathway of which the gene's expressed protein is a part.

WO 99/58720 provides methods for quantifying the relatedness of a first and second gene expression profile and for ordering the relatedness of a plurality of gene expression prof iles. The methods so described permit useful information to be extracted from a greater percentage of the individual gene expression measurements from a microarray than methods previously used in the art.

Other uses of microarrays are described in Gerhold et al., Trends Blochem. Scl. 24(5):168-173 (1999) and Zweiger, Trends Blotechnol. 17 (11):429- 436 (1999) Schena et al.

The invention particularly provides genomederived single-exon probes known to be expressed in heart.

The individual single exon probes can be provided in the form of substantially isolated and purified nucleic 61 acid, typically, but not necessarily, in a quantity sufficient to perform a hybridization reaction.

Such nucleic acid can be in any form directly hybridizable to the message that contains the probe's ORF, such as double stranded DNA, singlestranded DNA complementary to the message, singlestranded RNA complementary to the message, or chimeric DNA/RNA molecules so hybridizable. The nucleic acid can alternatively or additionally include either nonnative nucleotides, alternative internucleotide linkages, or both, so long as complementary binding can be obtained. For example, probes can include phosphorothioates, methylphosphonates, morpholino analogs, and peptide nucleic acids (PNA), as are described, for example, in U.S. Patent Nos. 5, 142,047; 5,235,033; 5,166,315; 5,217,866; 5,184,444; 5,861,250.

Usefully, however, such probes are provided in a form and quantity suitable for amplification, where the amplified product is thereafter to be used in the hybridization reactions that probe gene expression.

Typically, such probes are provided in a form and quantity suitable for amplification by PCR or by other well known amplification technique. One such technique additional to PCR is rolling circle amplification, as is described, inter alia, in U.S. Patent Nos. 5,854,033 and 5,714,320 and international patent publications WO 97/19193 and WO 00/15779. As is well understood, where the probes are to be provided in a form suitable for amplification, the range of nucleic acid analogues and/or internucleotide linkages will be constrained by the requirements and nature of the amplification enzyme.

Where the probe is to be provided in form suitable for amplification, the quantity need not be sufficient for direct hybridization for gene expression analysis, and need be sufficient only to function as an 62 amplification template, typically at least about 1, 10 or 100 pg or more.

Each discrete amplifiable probe can also be packaged with amplification primers, either in a single composition that comprises probe template and primers, or in a kit that comprises such primers separately packaged therefrom. As earlier mentioned, the ORF-specific 51 primers used for genomic amplification can have a first common sequence added thereto, and the ORF-specific 31 primers used for genomic amplification can have a second, different, common sequence added thereto, thus permitting, in this embodiment, the use of a single set of 51 and 31 primers to amplify any one of the probes. The probe composition and/or kit can also include buffers, enzyme, etc., required to effect amplification.

As mentioned earlier, when intended for use on a genome-derived single exon microarray of the present invention, the genome-derived single exon probes of the present invention will typically average at least about 100, 200, 300, 400 or 500 bp in length, including (and typically, but not necessarily centered about) the ORF. Furthermore, when intended for use on a genome-derived single exon microarray of the present invention, the genome-derived single exon probes of the present invention will typically not contain a detectable label.

When intended for use in solution phase hybridization, however - that is, for use in a hybridization reaction in which the probe is not first bound to a support substrate (although the target may indeed be so bound) length constraints that are imposed in microarray- based hybridization approaches will be relaxed, and such probes will typically be labeled.

In such case, the only functional constraint that dictates the minimum size of such probe is that each such 63 probe must be capable of specifically identifying in a hybridization reaction the exon from which it is drawn. In theory, a probe of as little as 17 nucleotides is capable of uniquely identifying its cognate sequence in the human genome. For hybridization to expressed message - a subset of target sequence that is much reduced in complexity as compared to genomic sequence - even fewer nucleotides are required for specificity.

Therefore, the probes of the present invention can include as few as 20, 25 or 50 bp or ORF, or more. In particular embodiments, the ORF sequences are given in SEQ ID NOS. 5,203 - 10,283, respectively, for probe SEQ ID NOS. 1 - 5,202. The minimum amount of ORF required to be included in the probe of the present invention in order to provide specific signal in either solution phase or microarray-based hybridizations can readily be determined for each of ORF SEQ ID NOS. 5, 203 - 10,283 individually by routine experimentation using standard high stringency conditions.

Such high stringency conditions are described, inter alia, in Ausubel et al. and Maniatis et al. For mi croarray- based hybridization, standard high stringency conditions can usefully be 50% formamide, 5X SSC, 0.2 gglgl poly(dA), 0.2 ggIM1 human c,tl DNA, and 0.5 % SDS, in a humid oven at 420C overnight, followed by successive washes of the microarray in 1X SSC, 0.2% SDS at 550C for 5 minutes, and then 0.1X SSC, 0.2% SDS, at 550C for 20 minutes. For solution phase hybridization, standard high stringency conditions can usefully be aqueous hybridization at 650C in 6X SSC. Lower stringency conditions, suitable for cross - hybridization to mRNA encoding structurally- and functionally-related proteins, can usefully be the same as the high stringency conditions but with reduction in temperature for hybridization and washing to room 64 temperature (approximately 25OC) When intended for use in solution phase hybridization, the maximum size of the single exon probes of the present invention is dictated by the proximity of other expressed exons in genomic DNA: although each single exon probe can include intergenic and/or intronic material contiguous to the ORF in the human genome, each probe of the present invention will include portions of only one expressed exon.

Thus, each single exon probe will include no more than about 25 kb of contiguous genomic sequence, more typically no more than about 20 kb of contiguous genomic sequence, more usually no more than about 15 kb, even more usually no more than about 10 kb. Usually, probes that are maximally about 5 kb will be used, more typically no more than about 3 kb.

It will be appreciated that the Sequence Listing appended hereto presents, by convention, only that strand of the probe and ORF sequence that can be directly translated reading f rom 51 to 31 end. As would be well understood by one of skill in the art, single stranded probes must be complementary in sequence to the ORF as present in an mRNA; it is well within the skill in the art to determine such complementary sequence. It will further be understood that double stranded probes can be used in both solution-phase hybridization and mi c roarray- based hybridization if suitably denatured.

Thus, it is an aspect of the present invention to provide single-stranded nucleic acid probes that have sequence complementary to those described herein above and below, and double-stranded probes one strand of which has sequence complementary to the probes described herein.

The probes can, but need not, contain intergenic and/or intronic material that flanks the ORF, on one or both sides, in the same linear relationship to the ORF that the intergenic and/or intronic material bears to the ORF in genomic DNA. The probes do not, however, contain nucleic acid derived from more than one expressed ORF.

And when intended for use in solution hybridization, the probes of the present invention can usefully have detectable labels. Nucleic acid labels are well known in the art, and include, inter alia, radioactive labels, such a S 3 H, 32 P ' 33 P ' 35S, 121, ' 131,; fluorescent labels, such as Cy3, Cy5, CyS.5, Cy7, SYBRO Green and other labels described in Haugland, Handbook of Fluorescent Probes and Research Chemicals, 7th ed., Molecular Probes Inc., Eugene, OR (2000), or fluorescence resonance energy transfer tandem conjugates thereof; labels suitable for chemiluminescent and/or enhanced chemiluminescent detection; labels suitable for ESR and NMR detection; and labels that include one member of a specific binding pair,such as biotin, digoxigenin, or the like.

The probes, either in quantity sufficient for hybridization or sufficient for amplification, can be provided in individual vials or containers.

Alternatively, such probes can usefully be packaged as a plurality of such individual genome-derived single exon probes.

When provided as a collection of plural individual probes, the probes are typically made available in amplifiable form in a spatial ly-addressable ordered set, typically one per well of a microtiter dish. Although a 96 well microtiter plate can be used, greater efficiency is obtained using higher density arrays.

If, as earlier mentioned, the ORF-specific 51 primers used for genomic amplification had a first common sequence added thereto, and the ORFspecific 31 66 primers used for genomic amplification had a second, different, common sequence added thereto, a single set of 51 and 31 primers can be used to amplify all of the probes from the amplifiable ordered set.

Such collections of genome-derived single exon probes can usefully include a plurality of probes chosen for the common attribute of expression in the human heart.

In such defined subsets, typically at least 50, 60, 75, 80, 85, 90 or 95% or more of the probes will be chosen by their expression in the defined tissue or cell type.

The single exon probes of the present invention, as well as fragments of the single exon probes comprising selectively hybridizable portions of the probe ORF, can be used to obtain the full length cDNA that includes the ORF by (i) screening of cDNA libraries; (ii) rapid amplification of cDNA ends ("RACE"); or (iii) other conventional means, as are described, inter alla, in Ausubel et al. and Maniatis et al.

It is another aspect of the present invention to provide genome-derived single exon nucleic acid microarrays useful for gene expression analysis, where the term llmicroarrayll has the meaning given in the definitional section of this description, supra.

The invention particularly provides genomederived single-exon nucleic acid microarrays comprising a plurality of probes known to be expressed in human heart. In preferred embodiments, the present invention provides human genome-derived single exon microarrays comprising a plurality of probes drawn from the group consisting of SEQ ID NOS.: 1 - 5, 202.

When used for gene expression analysis, the genome-derived single exon microarrays provide greater physical informational density than do the genome-derived 67 single exon microarrays that have lower percentages of probes known to be expressed commonly in the tested tissue. At a fixed probe density, for example, a given microarray surface area of the defined subset genome- derived single exon microarray can yield a greater number of expression measurements. Alternatively, at a given probe density, the same number of expression measurements can be obtained from a smaller substrate surface area. Alternatively, at a fixed probe density and fixed surface area, probes can be provided redundantly, providing greater reliability in signal measurement for any given probe. Furthermore, with a higher percentage of probes known to be expressed in the assayed tissue, the dynamic range of the detection means can be adjusted to reveal finer levels discrimination among the levels of expression.

Although particularly described with respect to their utility as probes of gene expression, particularly as probes to be included on a genomederived single exon microarray, each of the nucleic acids having SEQ ID NOS.: 1 - 5,202 contains an open-reading frame, set forth respectively in SEQ ID NOS.: 5,203 - 10,283, that encodes a protein domain. Thus, each of SEQ ID NOS. 1 - 5,202 can be used, or that portion thereof in SEQ ID NOS. 5,203 - 10,283 used, to express a protein domain by standard in vitro recombinant techniques. See Ausubel et al. and Maniatis et al.

Additionally, kits are available commercially that readily permit such nucleic acids to be expressed as protein in bacterial cells, insect cells, or mammalian cells, as desired (e.'g., HAT' Protein Expression & Purification System, ClonTech Laboratories, Palo Alto, CA; Adeno-X" Expression System, ClonTech Laboratories, Palo Alto, CA; Protein Fusion & Purification (pMALm) System, New England Biolabs, Beverley, MA) 68 Furthermore, shorter peptides can be chemically synthesized using commercial peptide synthesizing equipment and well known techniques. Procedures are described, inter alia, in Chan et al. (eds. Fmoc Solid Phase Peptide Synthesis: A Practical Approach (Practical Approach Series, (Paper)), Oxford Univ. Press (March 2000) (ISBN:

0199637245); Jones, Amino Acid and Peptide Synthesis (Oxford Chemistry Primers, No 7) Oxford Univ. Press (August 1992) (ISBN: 0198556683) and Bodanszky, Principles of Peptide Synthesis (Springer Laboratory), Springer Verlaq (December 1993) (ISBN: 0387564314).

It is, therefore, another aspect of the invention to provide peptides comprising an amino acid sequence translated from SEQ ID NOS.: 5,203 - 10, 283. Such amino acid sequences are set out in SEQ ID NOS: 10,284 - 15,322. Any such recombinantlyexpressed or synthesized peptide of at least 8, and preferably at least about 15. amino acids, can be conjugated to a carrier protein and used to generate antibody that recognizes the peptide. Thus. it is a further aspect of the invention to provide peptides that have at least 8, preferably at least 15, consecutive amino acids.

The following examples are offered by way of illustration and not by way of limitation.

EXAMPLE 1

Preparation of Single Exon Microarrays from ORFs Predicted in Human Genomic Sequence Bioinformatics Results All human BAC sequences in fewer than 10 pieces that had been accessioned in a five month period immediately preceding this study were downloaded from 69 GenBank. This corresponds to 2200 clones, totaling 350 MB of sequence, or approximately 10% of the human genome.

After masking repetitive elements using the program CROSS-MATCH, the sequence was analyzed for open reading frames using three separate gene finding programs. The three programs predict genes using independent algorithmic methods developed on independent training sets: GRAIL uses a neural network, GENEFINDER uses a hidden Markoff model, and DICTION, a program proprietary to Genetics Institute, operates according to a different heuristic. The results of all three programs were used to create a prediction matrix across the segment of genomic DNA.

The three gene f inding programs yielded a range of results. GRAIL identified the greatest percentage of genomic sequence as putative coding region, 2% of the data analyzed. GENEFINDER was second, calling 1%, and DICTION yielded the least putative coding region, with 0.8% of genomic sequence called as coding region.

The consensus data were as follows. GRAIL and GENEFINDER agreed on 0.7% of genomic sequence, GRAIL and DICTION agreed on 0.5% of genomic sequence, and the three programs together agreed on 0.25% of the data analyzed.

That is, 0.25% of the genomic sequence was identified by all three of the programs as containing putative coding region.

ORFs predicted by any two of the three programs ("consensus ORFs11) were assorted into "gene bins" using two criteria: (1) any 7 consecutive exons within a 25 kb window were placed together in a bin as likely contributing to a single gene, and (2) all ORFs within a 25 kb window were placed together in a bin as likely contributing to a single gene if fewer than 7 exons were found within the 25 kb window.

PCR The largest ORF from each gene bin that did not span repetitive sequence was then chosen for amplification, as were all consensus ORFs longer than 500 bp. This method approximated one exon per gene; however, a number of genes were found to be represented by multiple elements.

Previously, we had determined that DNA fragments fewer than 250 bp in length do not bind well to the amino- modified glass surface of the slides used as support substrate for construction of microarrays; therefore, amplicons were designed in the present experiments to approximate 500 bp in length.

Accordingly, after selecting the largest ORF per gene bin, a 500 bp fragment of sequence centered on the ORF was passed to the primer picking software, PRIMER3 (available online for use at http://www-genome.wi.mit.edu/cgi-bin/primer/ A first additional sequence was commonly added to each ORF-unique 51 primer, and a second, different, additional sequence was commonly added to each ORF-unique 31 primer, to permit subsequent reamplification of the amplicon using a single set of "universal" 51 and 31 primers, thus immortalizing the amplicon. The addition of universal priming sequences also facilitates sequence verification, and can be used to add a cloning site should some ORFs be found to warrant further study.

The ORFs were then PCR amplified from genomic DNA, verified on agarose gels, and sequenced using the universal primers to validate the identity of the amplicon to be spotted in the microarray.

Primers were supplied by Operon Technologies (Alameda, CA). PCR amplification was performed by standard techniques using human genomic DNA (Clontech, Palo Alto, 71 CA) as template. Each PCR product was verified by SYBR"' green (Molecular Probes, Inc., Eugene, OR) staining of agarose gels, with subsequent imaging by Fluorimager (Molecular Dynamics, Inc., Sunnyvale, CA). PCR amplification was classified as successful if a single band appeared.

The success rate for amplifying ORFs of interest directly from genomic DNA using PCR was approximately 75%. FIG. 5 graphs the distribution of predicted ORF (exon) length and distribution of amplified PCR products, with ORF length shown in red and PCR product length shown in blue (which may appear black in the figure). Although the range of ORF sizes is readily seen to extend to beyond 900 bp, the mean predicted exon size was only 229 bp, with a median size of 150 bp (n=9498). With an average amplicon size of 475 25 bp, approximately 50% of the average PCR amplification product contained predicted coding region, with the remaining 50% of the amplicon containing either intron, intergenic sequence, or both.

Using a strategy predicated on amplifying about 500 bp, it was found that long exons had a higher PCR failure rate. To address this, the bioinformatics process was adjusted to amplify 1000, 1500 or 2000 bp fragments from exons larger than 500 bp. This improved the rate of successful amplification of exons exceeding 500 bp, constituting about 9. 2% of the exons predicted by the gene finding algorithms.

Approximately 75% of the probes disposed on the array (90% of those that successfully PCR amplified) were sequence-verified by sequencing in both the forward and reverse direction using MegaBACE sequencer (Molecular Dynamics, Inc., Sunnyvale, CA), universal primers, and standard protocols.

Some genomic clones (BACs) yielded very poor PCR 72 and sequencing results. The reasons for this are unclear, but may be related to the quality of early draft sequence or the inclusion of vector and host contamination in some submitted sequence data.

Although the intronic and intergenic material flanking coding regions could theoretically interfere with hybridization during microarray experiments, subsequent empirical results demonstrated that differential expression ratios were not significantly affected by the presence of noncoding sequence. The variation in exon size was similarly found not to affect differential expression ratios significantly; however, variation in exon size was observed to affect the absolute signal intensity (data not shown).

The 350 MB of genomic DNA was, by the abovedescribed process, reduced to 9750 discrete probes, which were spotted in duplicate onto glass slides using commercially available instrumentation (MicroArray GenII Spotter and/or MicroArray GenIII Spotter, Molecular Dynamics, Inc., Sunnyvale, CA). Each slide additionally included either 16 or 32 E. coli genes, the average hybridization signal of which was used as a measure of background biological noise.

Each of the probe sequences was BLASTed against the human EST data set, the NR data set, and SwissProt GenBank (May 7, 1999 release 2.0.9).

one third of the probe sequences (as amplified) produced an exact match (BLAST Expect ("E") values less than 1 e-100) to either an EST (20% of sequences) or a known mRNA (13% of sequences). A further 22% of the probe sequences showed some homology to a known EST or mRNA (BLAST E values from 1 e-5 to 1 e-99) The remaining 45% of the probe sequences showed no significant sequence homology to any expressed, or potentially expressed, sequences 73 present in public databases.

All of the probe sequences (as amplified) were then analyzed for protein similarities with the SwissProt database using BLASTX, Gish et al., Nature Genet. 3:266 (1993) The predicted functional breakdowns of the 2/3 of probes identical or homologous to known sequences are presented in Table 1.

Table 1

Function of Predicted ORFs As Deduced From Comparative Sequence Analysis Total V6 chip V7 chip Function Predicted from Comparative Sequence Analysis 211 96 115 Receptor 43 77 Zinc Finger 11 19 Homeobox 9 16 Transcription Factor 17 11 7 Transcription 118 57 61 Structural 39 56 Kinase 36 18 18 Phosphatase 83 31 52 Ribosomal 19 26 Transport 21 17 14 Growth Factor 17 12 5 Cytochrome so 33 17 Channel As can be seen, the two most common types of genes were transcription factors and receptors, making up 2.2% and 1.8% of the arrayed elements, respectively.

74 EXAMPLE 2

Gene Expression Measurements From Genome-Derived Single Exon Microarrays The two genome-derived single exon microarrays prepared according to Example 1 were hybridized in a series of simultaneous two-color fluorescence experiments to (1) Cy3-labeled cDNA synthesized from message drawn individually from each of brain, heart, liver, fetal liver, placenta, lung, bone marrow, HeLa, BT 474, or HBL 100 cells, and (2) CyS-labeled cDNA prepared from message pooled from all ten tissues and cell types, as a control in each of the measurements. Hybridization and scanning were carried out using standard protocols and Molecular Dynamics equipment.

Driefly, mRNA samples were bought from commercial sources (Clontech, Palo Alto, CA and Amersham Pharmacia Biotech (APB)). Cy3-d= and CyS-dCTP (both from APB) were incorporated during separate reverse transcriptions of 1 pg of polyA' mRNA performed using 1 pg oligo(dT)12-18 primer and 2 gg random 9mer primers as follows. After heating to 700C, the RNA:primer mixture was snap cooled on ice. After snap cooling on ice, added to the RNA to the stated f inal concentration was: 1X Superscript II buffer, 0.01 M DTT, 1009M dATP, 100 gM dGTP, 100 gM dTTP, 50 gM dCTP, 50 gM Cy3-d=P or CyS-dCTP 50 gM, and 200 U Superscript II enzyme. The reaction was incubated for 2 hours at 420C. After 2 hours, the first strand cDNA was isolated by adding 1 U Ribonuclease H, and incubating for 30 minutes at 370C. The reaction was then purified using a Qiagen PCR cleanup column, increasing the number of ethanol washes to 5. Probe was eluted using 10 mM Tris pH 8. S.

Using a spectrophotometer. probes were measured for dye incorporation.

Volumes of both Cy3 and Cy5 cDNA corresponding to 50 pmoles of each dye were then dried in a Speedvac, resuspended in 30 gl hybridization solution containing 50% f ormamide, 5X SSC, 0. 2 gglgl poly (dA), 0. 2 gglgl human c,,tl DNA, and 0. 5 % SDS.

Hybridizations were carried out under a coverslip, with the array placed in a humid oven at 420C overnight. Before scanning, slides were washed in 1X SSC, 0.2% SDS at 55't for 5 minutes, followed by 0.1X SSC, 0.2% SDS, at 550C for 20 minutes. Slides were briefly dipped in water and dried thoroughly under a gentle stream of nitrogen.

Slides were scanned using a Molecular Dynamics Gen3 scanner, as described. Schena (ed.), Microarray Biochip: Tools and Technology, Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376).

Although the use of pooled cDNA as a reference permitted the survey of a large number of tissues, it attenuates the measurement of relative gene expression, since every highly expressed gene in the tissue/cell typespecific fluorescence channel will be present to a level of at least 10% in the control channel. Because of this fact, both signal and expression ratios (the latter hereinafter, "expression" or "relative expression") for each probe were normalized using the average ratio or average signal, respectively, as measured across the whole slide.

Data were accepted for further analysis only when signal was at least three times greater than biological noise, the latter defined by the average signal produced by the E. coli control genes.

The relative expression signal for these probes was then plotted as function of tissue or cell type, and is presented in FIG. 6.

76 FIG. 6 shows the distribution of expression across a panel of ten tissues. The graph shows the number of sequence-verified products that were either not expressed (11 0 11), expressed in one or more but not all tested tissues (11111 - 11911), and expressed in all tissues tested (111011).

Of 9999 arrayed elements on the two microarrays (including positive and negative controls and "failed" products), 2353 (51%) were expressed in at least one tissue or cell type. of the gene elements showing significant signal - where expression was scored as ',significant" if the normalized Cy3 signal was greater than 1, representing signal 5-fold over biological noise (0.2) - 39% (991) were expressed in all 10 tissues. The next most common class (15%) consisted of gene elements expressed in only a single tissue.

The genes expressed in a single tissue were further analyzed, and the results of the analyses are compiled in FIG. 7.

FIG. 7A is a matrix presenting the expression of all verified sequences that showed expression greater than 3 in at least one tissue. Each clone is represented by a column in the matrix. Each of the 10 tissues assayed is represented by a separate row in the matrix, and relative expression of a clone in that tissue is indicated at the respective node by intensity of green shading, with the intensity legend shown in panel B. The top row of the matrix (11EST Hit") contains llbioinformaticll rather than "physicaP' expression data - that is, presents the results returned by query of EST, NR and SwissProt databases using the probe sequence. The legend for llbioinformatic expression,' (i.e., degree of homology returned) is presented in panel C. Briefly, white is known, black is novel, with gray depicting nonidentical with significant 77 homology (white: E values < le-100; gray: E values from le05 to le-99; black: E values > le-05).

As FIG. 7 readily shows, heart and brain were demonstrated to have the greatest numbers of genes that were shown to be uniquely expressed in the respective tissue. In brain, 200 uniquely expressed genes were identified; in heart, 150. The remaining tissues gave the following figures for uniquely expressed genes: liver, 100; lung, 70; fetal liver, 150; bone marrow, 75; placenta, 100; HeLa, 50; HBL, 100; and BT474, 50.

It was further observed that there were many more "novel" genes among those that were up-regulated in only one tissue. as compared with those that were down-regulated in only one tissue. In fact, it was found that ORFs whose expression was measurable in only a single of the tested tissues were represented in sequencing databases at a rate of only 11%, whereas 36% of the ORFs whose expression was measurable in 9 of the tissues were present in public databases. As for those ORFs expressed in all ten tissues, fully 45% were present in existing expressed sequence databases. These results are not unexpected, since genes expressed in a greater number of tissues have a higher likelihood of being, and thus of having been, discovered by EST approaches.

Comparison of Signal from Known and Unknown Genes The normalized signal of the genes found to have high homology to genes present in the GenBank human EST database were compared to the normalized signal of those genes not found in the GenBank human EST database. The data are shown in FIG. 8.

FIG. 8 shows the normalized Cy3 signal intensity for all sequence veri f ied products with a BLAST Expect ("E") value of greater than le-30 (designated "unknown,') 78 upon query of existing EST, NR and SwissProt databases, and shows in blue the normalized Cy3 signal intensity for all sequence-verified products with a BLAST Expect value of less than le-30 Pknown"). Note that biological background 5 noise has an averaged normalized Cy3 signal intensity of 0.2.

As expected, the most highly expressed of the ORFs were "known,' genes. This is not surprising, since very high signal intensity correlates with very commonly- expressed genes, which have a higher likelihood of being found by EST sequence.

However, a significant point is that a large number of even the high expressers were "unknown". Since the genomic approach used to identify genes and to confirm their expression does not bias exons toward either the 31 or 51 end of a gene, many of these high expression genes will not have been detected in an end-sequenced cDNA library.

The significant point is that presence of the gene in an EST database is not a prerequisite for incorporation into a genome-derived microarray, and further, that arraying such "unknown,' exons can help to assign function to as-yet undiscovered genes.

Verification of Gene Expression To ascertain the validity of the approach described above to identify genes from raw genomic sequence, expression of two of the probes was assayed using reverse transcriptase polymerase chain reaction (RT PCR) and northern blot analysis.

Two microarray probes were selected on the basis of exon size, prior sequencing success, and tissue-specific gene expression patterns as measured by the microarray experiments. The primers originally used to amplify the 79 two respective ORFs f rom, genomic DNA were used in RT PCR against a panel of tissue-specific cDNAs (Rapid-Scan gene expression panel 24 human cDNAs) (OriGene Technologies, Inc., Rockville, MD).

Sequence AL0793001 was shown by microarray hybridization to be present in cardiac tissue, and sequence AL0317341 was shown by microarray experiment to be present in placental tissue (data not shown). RT-PCR on these two sequences confirmed the tissue-specific gene expression as measured by microarrays, as ascertained by the presence of a correctly sized PCR product from the respective tissue type cDNAs.

Clearly, all microarray results cannot, and indeed should not, be confirmed by independent assay methods, or the high throughput, highly parallel advantages of microarray hybridization assays will be lost. However, in addition to the two RT-PCR results presented above, the observation that 1/3 of the arrayed genes exist in expression databases provides powerful confirmation of the power of our methodology - which combines bioinformatic prediction with expression confirmation using genomederived single exon microarrays - to identify novel genes from raw genomic data.

To verify that the approach further provides correct characterization of the expression patterns of the identified genes, a detailed analysis was performed of the microarrayed sequences that showed high signal in brain.

For this latter analysis, sequences that showed high (normalized) signal in brain, but which showed very low (normalized) signal (less than 0.5, determined to be biological noise) in all other tissues, were further studied. There were 82 sequences that fit these criteria, approximately 2% of the arrayed elements. The 10 sequences showing the highest signal in brain in microarray hybridizations are detailed in Table 2, along with assigned function, if known or reasonably predicted.

Table 2

Function of the Most Highly Expressed Genes Expressed Only in Brain Microarray Normal Expressi Homology Gene Function Sequence ized on Ratio to EST as described by Name Signal present GenBank in GenBank AP000217-1 5.2 +7.7 High S-100 protein, b-chain, Ca 21 binding protein expressed in central nervous system AP0000471 2.3 High Unknown Function AC006548-9 1.7 High Similar to mouse membrane glyco-protein M6, expressed in central nervous system AC007245-5 1.5 High Similar to amphiphysin, a synaptic vesicle associated protein. Ref 21 L44140-4 1.2 +2.0 High Endothelial actin-binding 81 protein found in nonmuscle filamin AC004689-9 1.2 +3.5 High Protein Phosphatase PP2A, neuronal/ downregulates activated protein kinases AL031657-1 1.2 +3.0 High Unknown function/ Contains the anhyrin motif, a common protein sequence motif AC009266-2 1.1 +3.7 Low Low homology to the Synaptotagmin I protein in rat/present at low levels throughout rat brain AP000086-1 1.0 +2.7 Low Unknown, very poor homology to collagen AC004689-3 1.0 High Protein Phosphatase PP2A, neuronal/ downregulates activated protein kinases 82 Of the ten sequences studied by these latter confirmatory approaches, eight were previously known. of these eight, six had previously been reported to be important in the central nervous system or brain. The exon giving the highest signal (AP00217-1) was found to be the gene encoding an S100B Ca 2+ binding protein, reported in the literature to be highly and uniquely expressed in the central nervous system. Heizmann, Neurochem. Res. 9:1097 (1997).

A number of the brain-specific probe sequences (including AC006548-9, AC009266-2) did not have homology to any known human cDNAs in GenBank but did show homology to rat and mouse cDNAs. Sequences AC004689-9 and AC004689-3 were both found to be phosphatases present in neurons (Millward et al., Trends Biochem. Sci. 24 (5):186-191 (1999H. Two microarray sequences, AP000047-1 and AP000086-1 have unknown function, with AP000086-1 being absent from GenBank. Functionality can now be narrowed down to a role in the central nervous system for both of these genes, showing the power of designing microarrays in this fashion.

Next, the function of the chip sequences with the highest (normalized) signal intensity in brain, regardless of expression in other tissues, was assessed. In this latter analysis, we found expression of many more common genes, since the sequences were not limited to those expressed only in brain. For example, looking at the 20 highest signal intensity spots in brain, 4 were similar to tubulin (AC00807905; AF146191-2; AC007664-4; AF141912), 2 were similar to actin (AL035701-2; AL034402-1), and 6 were found to be homologous to glyceraldehyde-3 -phosphate dehydrogenase (GAPDH) (AL035604-1; Z86090-1; AC006064-L, AC006064-K; AC0356043; AC006064-L). These genes are often used as controls or housekeeping genes in microarray 83 experiments of all types.

Other interesting genes highly expressed in brain were a ferritin heavy chain protein, which is reported in the literature to be found in brain and liver (joshi et al., J. Neurol. Sci. 134 (Suppl):52 -56 (1995)), a result duplicated with the array. Other highly expressed chip sequences included a translation elongation factor 1 (AC0075644), a DEAD-box homolog (AL023804-4), and a Ychromosome RNA-binding motif (Chai et al., Genomics 49(2):283-89 (1998)) (AC007320-3). A low homology analog (AP00123-1/2) to a gene, DSCR1, thought to be involved in trisomy 21 (Down's syndrome), showed high expression in both brain and heart, in agreement with the literature (Fuentes et al., Mol. Genet. 4 (10):1935-44 (1995)).

As a further validation of the approach, we selected the BAC AC006064 to be included on the array. This BAC was known to contain the GAPDH gene, and thus could be used as a control for the ORF selection process. The gene finding and exon selection algorithms resulted in choosing 25 exons from BAC AC006064 for spotting onto the array, of which four were drawn from the GAPDH gene. Table 3 shows the comparison of the average expression ratio for the 4 exons from BAC006064 compared with the average expression ratio for 5 different dilutions of a commercially availableGAPDH cDNA (Clontech).

Table 3

Comparison of Expression Ratio, for each tissue, of GAPDH Control ( n 5) AC006064 (n = 4) Bone Marrow -1.81 + 0.11 -1.85 0.08 Brain -1.41 + 0.11 -1.17 + 0.05 BT474 1.85 + 0.09 1.66 + 0.12 84 Fetal Liver -1.62 + 0.07 -1.41 + 0.05 HBL100 1.32 + 0.05 2.64 + 0.12 Heart 1.16 + 0.09 1.56 + 0.10 HeLa 1.11 +0.06 1.30 + 0.15 Liver -1.62 + 0.22 -2.07 + Lung -4.95 + 0.93 -3.75 + 0.21 Placenta -3.56 + 0.25 -3.52 + 0.43 Each tissue shows excellent agreement between the experimentally chosen exons and the control, again demonstrating the validity of the present exon mining approach. In addition, the data also show the variability of expression of GAPDH within tissues, calling into question its classification as a housekeeping gene and utility as a housekeeping control in microarray experiments.

EXAMPLE 3

Representation of Sequence and Expression Data as a 11Mondrianll For each genomic clone processed for microarray as above-described, a plethora of information was accumulated, including full clone sequence, probe sequence within the clone, results of each of the three gene finding programs, EST information associated with the probe sequences, and microarray signal and expression for multiple tissues, challenging our ability to display the information.

Accordingly, we devised a new tool for visual display of the sequence with its attendant annotation which, in deference to its visual similarity to the paintings of Piet Mondrian, is hereinafter termed a 11Mondrianll. FIGS. 3 and 4 present the key to the information presented on a Mondrian.

FIG. 9 presents a Mondrian of BAC AC008172 (bases 2 5, 0 0 0 to 13 0, 0 0 0 shown), containing the carbamyl phosphate synthetase gene (AF154830.1). Purple background within the region shown as field 81 in FIG. 3 indicates all 37 known exons for this gene.

As can be seen, GRAIL II successfully identified 27 of the known exons (73%), GENEFINDER successfully identified 37 of the known exons (100%), while DICTION identified 7 of the known exons (19%).

Seven of the predicted exons were selected for physical assay, of which 5 successfully amplified by PCR and were sequenced. These five exons were all found to be from the same gene, the carbamyl phosphate synthetase gene (AF154830. 1).

The five exons were arrayed, and gene expression measured across 10 tissues. As is readily seen in the Mondrian, the five chip sequences on the array show identical expression patterns, elegantly demonstrating the reproducibility of the system.

FIG. 10 is a Mondrian of BAC AL049839. We selected 12 exons from this BAC, of which 10 successfully sequenced, which were found to form between 5 and 6 genes.

Interestingly, 4 of the genes on this BAC are protease inhibitors. Again, these data elegantly show that exons selected from the same gene show the same expression patterns, depicted below the red line. From this figure, it is clear that our ability to find known genes is very good. A novel gene is also found from 86.6 kb to 88.6 kb, upon which all the exon finding programs agree. We are confident we have two exons from a single gene since they show the same expression patterns and the exons are proximal to each other. Backgrounds in the following

86 colors indicate a known gene (top to bottom) red = kallistatin protease inhibitor (P29622) purple = plasma serine protease inhibitor (P05154) turquoise = al anti -chymotrypsin (P01011); mauve = 40S ribosomal protein (P08865). Note that chip sequence 8 and 12 did not sequence verify.

EXAMPLE 4

Genome-Derived Single Exon Probes Useful For Measuring Human Gene Expression The protocols set forth in Examples 1 and 2, supra, were applied to additional human genomic sequence as it became newly available in GenBank. 5,081 unique exons in the human genome that could be shown to be expressed at significant levels in heart tissue were identified.

These unique exons are within longer probe sequences. Each probe was completely sequenced on both strands prior to its use on a genome-derived single exon microarray; sequencing confirms the exact chemical structure of each probe. An added benefit of sequencing is that it placed us in possession of a set of single baseincremented fragments of the sequenced nucleic acid, starting from the sequencing primer 31 OH. (Since the single exon probes were first obtained by PCR amplification from genomic DNA, we were of course additionally in possession of an even larger set of single base incremented fragments of each of the 5,202 single exon probes, each fragment corresponding to an extension product from one of the two amplification primers.) The structures of the 5,202 unique single exon probes are clearly presented in the Sequence Listing as SEQ ID Nos.: 1 5,202.The 16 nt 5 1 primer sequence and 16 nt 87 31 primer sequence present on the amplicon are not included in the sequence listing. The sequences of the exons present within each of these probes is presented in the Sequence Listing as SEQ ID Nos.: 5,203 - 10, 283, respectively. It will be noted that some amplicons have more than one exon, some exons are contained in more than one amplicon.

As detailed in Example 2, expression was demonstrated by disposing the amplicons as single exon probes on nucleic acid microarrays and then performing twocolor fluorescent hybridization analysis; significant expression is based on a statistical confidence that the signal is significantly greater than negative biological control spots. The negative biological control is formed from spotted DNA sequences from a different species. Here, 32 sequences from E.Coli were spotted in duplicate to give a total of 64 spots.

For each hybridisation (each slide, each colour) the median value of the signal from all of the spots is determined. The normalised signal value is the arithmetic mean of the signal from duplicate spots divided by the population median.

Control spots are eliminated if there is more that a fivefold difference between each one of the duplicate spots raw signals.

The median of the signal from the remaining control spots is calculated and all subsequent calculations are done with normalised signals.

Control spots having a signal of greater than median + 2.4 (the value 2.4 is roughly 12 times the observed standard deviation of control spot populations) are eliminated. Spots with such high signals are considered to be "outliers".

The mean and standard deviation of the modified 88 control spot populations are calculated.

The mean + 3x the standard deviation (mean + (3SD)) is used as the signal threshold qualifier for that particular hybridisation. Thus, individual thresholds are determined for each channel and each hybridisation.

This means that, assuming that the data is distributed normally, there is a 99% confidence that any signal exceeding the threshold is significant.

The probes and their expression data are presented in Table 4, set forth respectively in Example 5. Example 5 presents the subset of probes that is significantly expressed in the human heart and thus presents the subset of probes that was recognized to be useful for measuring expression of their cognate genes in human heart tissue.

The sequence of each of the exon probes identified by SEQ ID NOS.: 5,203 to 10,283 was individually used as a BLAST (or, for SWISSPROT, BLASTX) query to identify the most similar sequence in each of dbEST, SwissProt (BLASTX), and NR divisions of GenBank. Because the query sequences are themselves derived from genomic sequence in GenBank, only nongenomic hits from NR were scored.

The smallest in value of the BLAST (or BLASTX) expect PIE11) scores for each query sequence across the three database divisions was used as a measure of the "expression novelty" of the probe's ORP. Table 4 is sorted in descending order based on this measure, reported as "Most Similar (top) Hit BLAST E Value". 29 out of the 5081 exons were found to have no similar sequences.

As sorted, Table 4 thus lists its respective probes (by 11AMPLICON SEQ ID NO.: 11 and additionally by the SEQ ID NO:. of the exon contained within the probe:11EXON SEQ ID NO.: 11) from least similar to sequences known to be 89 expressed (i.e., highest BLAST E value), at the beginning of the table, to most similar to sequences known to be expressed (i.e., lowest BLAST E value), at the bottom of the table.

Table 4 further provides, for each listed probe, the accession number of the database sequence that yielded the "Most Similar (top) Hit BLAST E Value", along with the name of the database in which the database sequence is found ("Top Hit Database Source").

Table 4 further provides SEQ ID NOS.

corresponding to the predicted amino acid sequences where they have been determined for the probe and exon nucleotide sequences. These are set out as PEPTIDE SEQ ID NOS. The peptide sequences for a given exon are predicted as follows: Since each chip exon is a consensus sequence drawn from predictions from various exon finding programs (i.e. Grail, GeneFinder and GenScan), the multiple initial ORFs are first determined in a uniform way according to each prediction. In particular, the reading frame for predicting the first amino acid in the peptide sequence always starts with the first base of any codon and ends with the last base of non-termination codon. Next, for each strand of the exon, initial ORFs are merged into one or more final ORFs in an exhaustive process based on the following criteria:

1) the merging ORFs must be overlapping, and 2) the merging ORFs must be in the same frame.

The Sequence Listing, which is a superset of all of the data presented in Table 4, further includes, for each probe, the most similar hit, with accession number and BLAST E value, f rom the each of the three queried databases.

Table 4 further lists, for each probe, a portion of the descriptor for the top hit ("Top Hit Descriptorll) as provided in the sequence database. For those ORFs that are similar in sequence, but nonidentical to known sequences (e.g.. those with BLAST E values between about le-05 and le-100), the descriptor reveals the likely function of the protein encoded by the probe's ORF.

Using BLAST E value cutoffs of le-05 (i.e., 1 x 105) and le-100 (i.e., 1 x 10-100) as evidence of similarity to sequences known to be expressed is of course arbitrary:

in Example 2, supra, a BLAST E value of le-30 was used as the boundary when only two classes were to be defined for analysis (unknown, >le-30; known <le-30) (see also FIG. 8).

Furthermore, even when the "Most Similar (Top) Hit BLAST E Value', is low, e.g., less than about le-100 - which is probative evidence that the query sequence has previously been shown to be expressed - the top hit is highly unlikely exactly to match the probe sequence.

First, such expression entries typically will not have the intronic and/or intergenic sequence present within the single exon probes listed in the Table. Second, even the ORF itself is unlikely in such cases to be present identically in the databases, since most of the EST and mRNA clones in existing databases include multiple exons, without any indication of the location of exon boundaries.

As noted, the data presented in Table 4 represent a proper subset of the data present within the attached sequence listing. For each amplicon probe (SEQ ID N0s.: 1 - 5,202) and probe exon (SEQ ID N0s.: 5, 203 - 10, 283, respectively), the sequence listing further provides, through iterated annotation fields <220> and <223>:

(a) the accession number of the BAC from which the sequence was derived ("MAP TW), thus providing a link to the chromosomal map location and other information about the genomic milieu of the probe sequence; (b) the most similar sequence provided by BLAST query of the EST database, with accession number and BLAST Table 4

AMPLICON EXON PEPTIDE EXPRESSION HIT HIT ACCESSION DATASET HIT DESCRIPTION

SEQ ID SEQ SEO ID EVALUE NO: 0 NO: NO:

286 5473 10603 0.7 287 5473 10603 0.7 442 5608 1.5 871 6019 11179 5.8 1029 6169 0 ' 7 1280 6410 11571 2.7 1597 6723 11893 0,7 1619 6745 11913 1.8 1694 6818 11999 1.0 1715 6838 12017 1.0 1721 6844 12023 2.7 1848 6966 12152 1.0 1935 7052 12245 1.1 2119 7230 12439 10 2233 7340 12552 0.8 2893 8044 13144 5,9 3003 8155 13255 1.0 3003 8155 13256 1.0 3149 8298 13407 3.7 3403 8545 13653 1.5 11 3471 8612 13724 9.0 3513 8654 0.9 3607 8746 13841 1.0 4102 9228 14313 1.7 4164 9290 14370 5.9 4184 9310 14390 0.9 4184 9310 14391 0.9 4242 9367 1.0 4730 9842 14934 1.8 4779 9889 0.8 4976 10079 15149 5.1 4988 10091 15161 11 578 5738 10854 0.7 9.8OE+00 AA442238.1 EST-HUMAN zv61901,sl Soares_testis_NI-IT Homo sapiens cDNA done MAGE:758160 3' 2631 T721 12921 1.0 9.40E+00 L11433.1 NT Dengue virus type 3 membrane protein (prlenvelope glycoprotein (E) polyprotein mRNA, partial cds 26t- 7721 12922 1.0 9.40E+00 L1 1433.1 NT Dengue virus type 3 membrane protein (prWMyenvelope glycoprotein (E) polyprotein mRNA, partial cds Page 1 of 196 Table 4

433 5600 10731 0.6 8.40E+00 5031804 NT Homo sapiens insulin receptor substrate 1 (IRS1) mRNA 2947 8098 13203 4.2 T20E+00 L12051.1 NT Lycopersicon esculentum Mill. GTPase (SAR2) mRNA, complete cds 2947 8098 13204 4.2 7.2OE+00 L12051.1 NT Lycopersicon esculentum Mill. GTPase (SAR2) mRNA, complete cds 3479 8620 0.8 5,8OE+00 7661557 NT Homo sapiens DESC1 protein (DESC1), mRNA 4675 9787 14886 1.5 5.30E+00 L43126.1 NT Bovine immunodeftefency-like virus surface envelope gene, Vend of cds 3972 9104 9.4 4.8OE+00 AF185255.1 NT Eunice australis histone H3 (H3) gene, partial cds 3236 8384 13490 4.0 4.7OE+00 AL163280.2 NT Homo sapiens chromosome 21 segment HS21 C080 3459 8600 13713 3,9 3.9OE+00 X64518.1 NT N.tabacum chitinase gene 50 for class 1 chifinase C 4226 9350 1.0 3.9OE+00 AF055466.1 NT Mus musculus seminal vesicle secretory protein 99 (MSVSP99) gene, promoter region 2588 7681 0.9 3.8OE+00 AE001562.1 NT Helicobacter pylori, strain J99 section 123 of 132 of the complete genome 3936 9070 14154 10,1 3.7OE+00 AL161539.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 39 3946 9079 5.1 3.7OE+00 AF014880.1 NT Enterobacter agglomerans ColEl -like plasmid RNA one modulator (rom) gene, complete cds 3209 8357 13466 1.0 3.5OE+00 AF221538.1 NT Cryptospoddium fells heat shock protein 70 (HSP70) gene, partial cds 1496 6622 11792 1.3 3.4OE+00 AF254577.1 NT Brassica napus RID135d mRNA, complete cds 2532 7628 12830 1.0 3.4OE+00 AL163278.2 NT Homo sapiens chromosome 21 segment HS21 C078 5036 10136 15211 1.4 3.3012+00 7662155 NT Homo sapiens KIAA0480 gene product (K1AA0480), mRNA 5036 10136 15212 1.4 3.3OE+00 7662155 NT Homo saplens KIAA0480 gene product (K1AA0480), mRNA 492 5657 10780 1,4 3.2OE+00 X96422.1 NT D.rerio ze-50 POU gene 3938 5657 10780 0.8 3.2OE+00 X96422.1 NT Rrerjo zp-50 POU gene 4482 9602 1,5 3.2OE+00 AJ403571.1 NT M.musculus DNA for WAP-binding fragment GFAPB27 2807 7959 13067 1.1 3.0OE+00 8923984 NT Homo sapiens hypothetical protein PROO889 (PROO889), mRNA 1964 7080 12279 0.9 2.9OE+00 AE002225.2 NT Chlarnydophila pneumoniae AR39, section 53 of 94 of the complete genome 2114 7225 12433 1.1 2.9OE+00 AF055477.1 NT Rattus norvegicus pore-forming calcium channel alpha-1 B subunit variant a mRNA, alternatively spliced product, complete cds 1441 6568 11744 2.1 2.8DE+00 AF186398.1 NT Buxus harlandll maturase K (matK) gene, partial cds; chloroplast gene for chloroplast product 161.5 6741 1.1 2.8OE+00 AL161552.2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 52 230 5421 10544 1,6 2.7OE+00 6679306 NT Mus musculus per-hexamer repeat gene 3 (Phxr3), mRNA 230 5421 10545 1.6 2.7OE+00 6679306 NT Mus musculus per-hexamer repeat gene 3 (Phxr3), mRNA 2923 8074 13182 1.5 2.7OE+00 4504548 NT Homo saplens hexabrachion (tenascin C, cytotactin) (HXB) mRNA 3904 9039 14130 1.5 2.7OE+00 AF195052.1 NT Danio rerio proteasome activator subunit 1 (psmel) mRNA, complete eds 461 5627 10754 1.0 2.6OE+00 AE002369.1 NT Neisseria meningifidis serogroup B strain MC58 section 11 of 206 of the complete genome 461 5627 10755 1.0 2.6OE+00 AE002369.1 NT Nusseria meningifidis serogroup B strain MC58 section 11 of 206 of the complete genome 3526 8667 13771 0.8 2.6OE+00 AF111168.2 NT Homo sapiens serine paimitoyl transferase, subuffit 11 gene, complete cds; and unknown genes 3732 8869 13961 0.8 2.6OE+00 AW966084.1 EST-HUMAN EST378157 MAGE resequences, MAGI Homo sapiens cDNA 373 8869 13962 0.8 2.6OE+00 AW966084.1 EST-HUMAN EST378157 MAGE reseque2ces, MAGI Homo sapiens cDNA 45T '-96-93 14785 4.4 2.6OE+00 AF068749.1 NT Mus musculus sphingosine kinase (SPHKIb) mRNA, complete eds Page 2 of 196 Table 4 rvi 1;7 1448 6575 11748 0.8 2.5OE+00 AJ271844.1 NT AspeTillus nidulans recQ gene for DNA helicase. exons 1-4 1448 6575 11749 0.8 2.5OE+00 AJ271844.1 NT Aspergil(us nidulans recQ gene for DNA helicase, exons 1-4 2503 7600 0.8 2.4OE+00 P19532 SWISSPROT TRANSCRIPTION FACTOR E3 2980 8131 13239 0.9 2.4OE+00 M24282.1 NT Chicken alpha-3 collagen type V1 MRNA, Yend 3201 8349 13462 3.0 2.4OE+00 068906 SWISSPROT TRYPTOPHAN SYNTHASE ALPHA CHAIN 4816 9925 15017 7.6 2.4OE+00 4503352 NT Homo sapie s double C2-like domains, alpha (DOC2A) mRNA 1233 6362 11524 32 2.3OE+00 Z46724.1 NT G.domesticus artificial single chain antibody gene (L3) 1371 6499 11665 0.8 2.3012+00 AF180891.1 NT Danio redo fast skeletal muscle myosin fight polypeptide 3 (myiz3) mRNA, co 2639 7728 1.2 2,30E+00 AL163209.2 NT Horno sapi;ns chromosome 21 segment HS21C009 4031 9159 1,3 2.3OE+00 AJ401081.1 NT Bos taurus partial WW gene for gitochrome b 4216 9340 14423 3.8 2.2OE+00 D67071.1 NT Rat gene for requcalcin, exonll (non-coding exon) 4216 9340 14424 3.8 2.2OE+00 D67071.1 NT Rat gene for requealcin, exonl (non-coding exon) 557 7892 10836 2.3 2.1012+00 AF132612.2 NT Mus musculus pre-T cell receptor alpha gene, enhancer region and upstream region 830 5979 11140 0.8 2.1012+00 6912721 NT Homo sapiens tolloid-like 1 (TLLI), mRNA 3539 8680 1.2 2.1OE+00 AW449366.1 EST-HUMAN Ut-H-B13-aki-e-08-0-Ul.sl NCLCGAP_SubS Homo sapiens cIDNA done IMAGE:2734550 3' 3715 8852 13942 1.6 2.10E+00 5835149 NT Gorilla gorilla mitochonddon, complete genome 3715 8852 13943 1,6 2.1OE+00 5835149 NT la mitochonddon, complete genome 3785 8922 14016 1.3 2.1 OE+00 AF257470.1 NT Equus caballus calcitonin gene related peptide 11 precursor, mRNA, complete cds 1178 6311 11466 1.2 2.OOE+00 AF180527.1 NT Horno sapiens p22DoWel (DOKDEL) mRNA, complete cds 1178 6311 11467 1,2 2.0OE+00 AF180527.1 NT Homo sapiens p22DoWel (DOKDEL) mRNA, complete ods 1313 6441 11608 1.4 2.OOE+00 AF204927.1 NT Oryctolagus cuniculus Na+,K- ATPase beta 1 subunit mRNA, complete cds 1557 6684 1.1 2.00IE+00 P25582 SWISSPROT PUTATIVE RRNA METHY1-TRANSFERASE SPB1 2102 7213 12422 1.7 2.OOE+00 Z78279.1 NT R.norvegicus mRNA for collagen alphal type 1 2102 7213 12423 1.7 2.0012+00 Z78279.1 NT R.norvegicus mRNA for collagen alphal type 1 4011 9142 14224 2.1 2.OOE+00 AW664496.1 EST-HUMAN hil 3c05.xl NCICGAP_GU1 Homo sapiens eDNA clone IMAGE:2972168 X similar to gb:X01677 GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER (HUMAN); 4011 9142 14225 2.1 2.OOE+00 AW664496.1 EST HUMAN h113c05.xl NCLWAP_GUI Horno, saplens cDNA done IMAGE:2972168 Ysimilar to gb:X01677 GLYCERALDEHYDE 3-PHOSPHAT-E DEHYDROGENASE, LIVER (HUMAN); 1990 7106 0.7 1,13OE+00 AJ010045.1 NT Mus musculus mRNA for Rho quanine nucleofide-exchange factor, splice variant NET1A 3054 8205 13305 1 7 1.8OE+00 P21004 SWISSPROT PROTEIN B8 PRECURSOR 3082 8233 13331 4.5 1,80IE+00 U04356.1 NT Synechococcus sp. PCC7942 copper transporting P-ATPase (ctaA) and ATP synthase epsilon subunit (atpE) genes, complete cds 3082 8233 13332 4.5 1.8OE+00 U04356.1 NT Synechococcus sp. PCC7942 copper transporting P-ATPase (ctaA) and ATP synthase epsilon subunit (atpE) genes, complete cds 564 5 10B42 0.6 1.70E+00 014137 SWISSPROT HYPOTHETICAL PROTEIN KIAA0124 M 5726 10843 06 1.70E+00 Q14137 SWISSPROT HYPOTHETICAL PROTEIN KIAA0124 565 5726 10842 1.1 1.70E+00 Q14137 SWISSPROT HYPOTHETICAL PROTEIN KIAA0124 56 5726 10843 1.1 1.70E+00 Q14137 SWISSPROT HYPOTHETICAL PROTEIN KIAA0124 Page 3 of 196 Table 4

1092 6230 11381 0.7 1.70E+00 Q60114 SWISSPROT LEVANSUCRASE (BETA-DFRUCTOFURANOSYL TRANSFERASE) (SUCROSE 6 FRUCTOSYL TRANSFERASE) 2225 7332 12545 1.2 1.70E+00 AL163280.2 NT Homo sapiens chromosome 21 segment HS21C080 2321 7424 12633 1.0 1.70E+00 A1141067.1 EST HUMAN oz43h05.xl Soares Nhill- IMP4_SI Homo saplens cIDNA done IMAGE:1678137 3, 4356 9478 14569 0.8 1.70E+00 Q60114 SWISSPROT LEVANSUCRASE (BETA-D- FRUCTOIFURANOSYL TRANSFERASE) (SUCROSE 6 FRUCTOSYL TRANSFERASE) 5059 10157 1.1 1.70E+00 AA747059.1 EST HUMAN nx65c08.sl NCI_WAP-AW1 Homo sapiens cDNA clone IMAGE:1267118 similar to gb:D00723 GLYCINE CLEAVAGE SYSTEM H PROTEIN PRECURSOR (HUMAN); 1989 7104 12307 6.1 1.60E+00 AF199339.1 NT Homo sapiens lens epithelium- derived growth factor gene, alternatively spliced, complete cels 1997 7113 12315 1.3 1.6OE+00 AF077374.1 NT Homo sapiens small proline- rich protein (SPRR3) gene, exons 1, 2, and 3 and complete eds 2003 7118 12320 0.7 11.60E+00 Y1 1344.1 NT Mus musculus ST6GaINAciii gene, exon 2 2238 7344 0.5 11.60E+00 X98373.1 NT B.napus gene encoding endo- polygalacturonase 2931 8082 13190 2.5 11.60E+00 W58426.11 EST HUMAN zd25f01.rl Soares fetal' heart-NbHH19W Homo sapiens cDNA done IMAGE:341689 5' similar to gb:D29805 N-ACETYLLACTOSAMINE SYNTHASE (HUMAN); 3795 8932 14026 1.3 1.6OE+00 D64006,1 NT Synechocystis sp. PCC6803 complete genome, 25127, 3138604-3270709 4255 9380 14460 1.4 AF155827.1 NT Homo sapiens proliferation-associated SNF2-like protein (SMARCA6) mRNA, complete cds 4255 9380 14461 1.4 11.60E+00 AF1155827.1 NT Homo sapiens proliferationassoclated SNF2-like protein (SMARCA6) mRNA, complete eds 4359 9481 14572 1.2 11.60E+00 U34070.1 NT Human CCAATIenhancer binding protein alpha gene, complete cels 5016 10118 15189 3.1 11.60E+00 Y1 1344.1 NT Mus muscuius ST6GaINAcill gene, exon 2 5016 10118 15190 3.1 1.13OE+00 Y1 1344.1 NT Mus musculus ST6GaINAcill gene, exon 2 5239 10344 1.5 11.50E+00 U53449.11 NT Rattus norvegicus jun dimerization protein 2 Ocip-2) mRNA, complete cds 231 5422 10546 0.6 11.50E+00 AE002201.2 NT Chlamydophila pneumoniae AR39, section 32 of 94 of the complete genome 605 5763 0.6 1.5OE+00 6752961 NT Mus musculus a disintegrin and metalloproteinase domain (ADAM) 15 (metargidin) (Adam15), mRNA 2359 7461 12667 0.9 11.50E+00 A.1131402.1 NT Potato virus A RNA complete genome, isolate U 2468 7566 12769 0.7 1.50E+00 6678350 NT Mus musculus T-cell lymphoma Invasion and metastasis 1 (Tiaml), mIRNA 3099 7461 12667 2.2 11.50E+00 AJ131402.1 NT Potato virus A RNA complete genome, Isolate U 3329 8473 13586 0.8 1.5OE+00 AE001945.1 NT Deinococcus radiodurans R1 section 82 of 229 of the complete chromosome 1 27 5236 10340 1.3 1.40E+00 7661685 NT Homo sapiens DKFZP586M0722 protein (DKFZP586M0122), mRNA 27 5236 10341 1.3 11.40E+00 7661685 NT Homo saplens DKFZP586M0122 protein (DKFZP586M0122), mRNA 2231 7338 1,0 1.40E+00 AF053357.1 NT Helicobacter g)dori glutamine synthetase (ginA) gene, complete cds 2286 7391 3.4 11.40E+00 U67922.11 NT Ovis aries prion protein gene, complete ods 2634 7723 12926 0.6 1.40E+00 X74463.1 NT Human papillomavirus type 7 genomic DNA 2746 7830 13032 1.2 11.40E+00 AF064564.2 NT Fugu rubripes neurofibromatosis type 1 (NFl), A-kinase anchor protein (AKAP84), BAW protein (BAW), and WS131 protein (WSBI1) genes, complete ods t 13033 1.2 1.40E+00 AF064564.2 NT Fugu rub(jpes neurofibromatosis type 1 (NFI), A-kinase anchor protein (AKAP84), BAW 27r '7830 protein (BAW), and WS131 protein (WSBI1) genes, comple cds Page 4 of 196 Table 4 n Z_ 3034 8186 13287 1.0 1.40E+00 AE002324.2 NT Chlamydia muridarum, section 55 of 85 of the complete genome 3034 8186 13288 1.0 1.40E+00 AE002324.2 NT Chlamyd a muridarum. section 55 of 85 of the complete genome 3291 8437 0,9 1.40E+00 5453733 NT Homo sapiens Mad4 homolog (MAD4) mRNA 4162 9288 14367 1,1 1.40E+00 AW900455.1 EST HUMAN CMO-NN1005-140300-286- h06 NN1005 Horno sapiens cDNA 4162 9288 14368 1.1 1.40E+00 AW900455.1 EST HUMAN CM0-NW005-140300-286- h06 NN1005 Homo saplens cDNA 5137 10232 0.8 1.40E+00 Q07869 SWISSPROT PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA (PPAR-ALPHA) 248 5436 10562 0.6 1.30E+00 U67587.1 NT Methanococcus jannaschil section 129 of 150 of the complete genome 248 5436 10563 0.6 1.30E+00 U67587.1 NT Methanococcus jannaschil section 129 of 150 of the complete genome 558 5720 0.6 1.30E+00 Z73640.1 NT M.mucedo gene encoding 4-Dihydromethyl- tdsporate dehydrogenase 572 78W 0.8 1.30E4.00 AL163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 884 6032 11191 0.9 11.30E+00 AJ271192.1 NT Cantharellus sp. partial 25S rRNA gene, isolate Tibet 1113 6250 5.6 1.30E+00 Y1 9213.1 NT Homo saplens putative psihHbA pseudogene for hair keratin, exons 2 to 7 1275 6405 11565 3.7 1.30E+00 4507998 NT Homo sapiens zinc finger protein 157 (HZF22) (ZNFl 57) mRNA 1275 6405 11566 3,7 1.30E+00 4507998 NT Homo sapiens zirfc finder protein 157 (HZ1F22) (ZNFl 57) mRNA 1334 6463 0.6 1.30E+00 U61730.2 NT Colx lacryma obi ' dihydrodipicolinate synthase (dapA) gene, complete cds 1361 6489 1.4 1.3012+00 A1375930.1 EST HUMAN tc24e03.xl Soares - total fetus_Nb2HF8-gw Homo sapiens cDNA clone IMAGE:2065564 3' similar to contains Alu repetitive element;contains element MER22 repetitive element; 1593 6719 0.8 1.30E+00 AE002338.2 NT Chlamydia muridarum, section 66 of 85 of the complete genome 2196 7305 2.1 1,30E+00 A13030447.1 NT Cyprinus carplo M ' RPb and MASPb genes for mannose-binding lectin-associated serine protease (MASP) and MASP-related protein, complete cds 2909 8059 13164 1.1 1.30E+00 6755621 NT Mus musculus alpha-spectrin 1, erythrold (Spnal), mIRNA 3547 8688 13790 1,0 1.30E+00 AF016494.1 NT Fugu rubripes gamma- aminobutyric acid receptor beta subunit gene, partial cds; 55M erythrocyte membrane protein (P55), synaptic veside-assoclated integral membrane protein (VAMP-1), procollagen C-proteinase enhancer protein (PC6LCE) genes, complete c> 3902 9037 14128 1.0 1.30E+00 D90908.1 NT Synechocysfis sp. PCC6803 complete genorne, 10127,1188886-1311234 4502 8059 13164 0,8 1.30E+00 6755621 NT Mus musculus alpha-spectrin 1, erythroid (Spnal), mRNA 4623 9737 14833 1,3 1.30E+00 AL161472.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 2 4964 10067. 15139 1.0 1.3012+00 A.1252087.1 NT Plasmodium relchenovA partial 83/AMA-1 gene for apical membrane antigen 1 4964 10067 15140 1.0 1.30E+00 A.1252087.1 NT Plasmodium relchenovA partial 83/AMA-1 gene for apical membrane antigen 1 634 5793 10916 2.8 1.20E+00 AA676246.1 EST-HUMAN zj22d08.sl Soares- fetaLliveLspleen_l NKS-S1 Horno sapiens cDNA done IMAGE:431535 3' 806 5957 11110 1.3 1.20E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR(CLONE PFI-IRP.111) 806 5957 11111 1.3 1.20E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFI-11RP-111) 806 5957 11112 1.3 1.20E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFI-IRP-111) 860 6008 0.8 1.20E+00 8924234 NT Homo sap! ns hypothetical protein PR03077 (PR03077), mRNA 1145 6280 11433 1.9 1.20E+00 AF080245.2 Elaels oleifera sesquiterpene synthase mRNA, complete cds 1187 6319 11475 0.6 1.2 + NT pea seed-bome mosaic virus complete genome 1187 6319 11476 0.6 1.20E+00 AJ252242.1 NT pea seed-bome mosaic virus complete genorne 1 7079 12278 1.1 1 1 AF140631.1 NT Homo sapiens G-protein coupled receptor 14 (G13R14) gene, complete cds 1.20E+00 Page 5 of 196 Table 4

3072 8223 13321 1.0 1.20E+00 AB020681.1 NT Homo sapiens mRNA for KIAA0874 protein, partial cds 3128 8277 13383 5.4 1.20E+00 AL161563.2 NT kabldopsis thaliana DNA chromosome 4, config fragment No. 63 3128 8277 13384 5.4 1.20E+00 AL161563.2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 63 3249 8396 2.9 1.20E+00 P54910 SWISSPROT CONJUGAL TRANSFER PROTEIN TRBE PRECURSOR 3311 8456 13570 0.8 1.20E+00 AF188740.1 NT Homo saplens LHX3 gene, intron 2 3312 8457 1.0 11.20E+00 M81779.1 NT G.gallus T-cadherin mRNA, complete ods 3650 8789 13882 7.3 1.20E+00 U75902.1 NT Mus musculus subdiisin-like serine protease 1-PC (PC7) gene, exons 1 to 9, partial cds 4201 8456 13570 1.1 1.20E+00 AF1 88740.1 NT Homo sapiens LHX3 gene, Intron 2 4298 9422 14513 1.4 1.20E+00 6980951 NT Rattus norvegicus Glydne receptor alpha 2 subuffit (glycin receptor, neonatal) (Gira2), mRNA 4371 9493 1.6 1.20E+00 M87060.1 NT Rattus rattus cardiac AE3 gene, exons1-23 4422 9542 14634 1.3 1.20E+00 AL161509.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 21 4460 9580 14677 1.8 1.20E+00 AF156495.1 NT Homo sapiens post-synapfic density 95 (DI-G4) gene, complete cds 4485 9605 5.4 1.20E+00 Y09200.1 NT T.pinnatum chloroplast rbcL gene, partial 4584 8457 0.9 1.20E+00 M81779.1 NT G.gallus T-cadherin mRNA, complete cds 456 5622 10749 1.1 1.10E+00 D86980.1 NT Human mRNA for KIAA0227 gene, partial cds 1726 6849 12028 1.4 1.1OE+00 AW995393.1 EST HUMAN QVO-BN0042-170300-163- g12 BN0042 Homo saplens cDNA 1857 6975 12160 0.9 1.1OE+00 AW575889.1 EST HUMAN U]-HF-BROp-ajk-f-02-0- UlM NIH_WC-52 Homo sapiens cDNA clone IMAGE:3074834 3, 2870 8021 13123 1.0 1.1OE+00 AB040955.1 NT Homo sapiens mRNA for KIAA1522 protein, partial ods 2971 8122 13229 0.8 1.10E+00 4501940 NT Homo sapiens alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide (ADH7) mRNA 2971 8122 13230 0.8 1.10E+00 4501940 NT Horno sapiens alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide (ADH7) mRNA 3288 8433 13541 6.6 1.1OE+00 AL163213,2 NT Homo sapiens chromosome 21 segment HS21 C01 3 3288 8433 13542 6.6 1.1OE+00 AL163213,2 NT Homo sapiens chromosome 21 segment HS21 C01 3 3441 8583 13697 0.9 1. 1 O-E+00 8922641 NT Homo saplens hypothetical protein FI-J1074.9 (FLJ10749), mRNA 3468 8609 13721 1.0 1.1OE+00 N84066.1 EST HUMAN KK4629F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA clone KK4629 5' similar to ORF(RELATED TO ACTIN BUNDLING PROTEIN DEMATW) 3510 8651 1.3 1, 1 OE+00 8922973 NT Homo sapiens hypothetical protein FLJ 11280 (FLJ 11280), mRNA 3531 8672 13776 1.0 1.1OE+00 A1808360.1 EST-HUMAN w154h11.xl Soares_M - T - GBC - S1 Horno sapiens eDNA done IMAGE:2359461 3'similar to SW:P531 HUMAN Q12888 P53-BINDING PROTEIN 53BP1 3657 8796 13889 1.2 1.1OE+00 Aú003886.1 NT Xylella fasddiosa, section 32 of 229 of the complete genome 3657 8796 13890 1.2 1.1012+00 AE003886.1 NT Xyleifa fasfidiosa, section 32 of 229 of the complete genome 3678 8817 13910 17.8 1.1OE+00 5729757 NT Homo sapiens calpain 9 (nCL-4) (CAPN9) mRNA 3862 8998 14097 1.0 1.1OE+00 8922641 NT Homo sapiens hypothetical protein FLJ10749 (FI-J10749), mRNA 4122 9248 5.9 1. 1 OE+00 5835331 NT R.unlcomis wrnplete mltochonddal genorne 4860 9967 15052 1.2 1.1OE+00 L23195.1 NT Drosophila melanogaster cytoplasmic dynein heavy chain mRNA, complete cds 4921 10028 15105 2.9 1.1OE+00 U18466.1 NT African svAne fever virus, complete genome 4999 10102 15170 1.2 1.10E+00 X78425.1 NT E.faecalls pbp5 gene 112- T 5-29-9 1,2 1.OOE+00 U23808.1 NT Xenopus laevis rhodopsin gene, complete cds Page 6 of 196 Table 4

108 5311 10436 1.1 11.00IE+00 D88425.11 NT Cavila cobaya mRNA for sednelthreolne kinase, complete cds 412 5580 0.7 1.00E+00 AB021684.1 NT Marchanda polymorpha genes for 26S rRNA, 5S rRNA, 18S rRNA, 5.8S rRNA and 26S rRNA 562 5724 10840 1.1 1.OOE+00 A.1251660.1 NT Girardia figrina mRNA for homeodomain transcription factor (so gene) 661 5817 10945 1.5 11.00E+00 A1.163218.2 NT Homo sapiens chromosome 21 segment HS21 C01 8 663 5819 1.0 11.00IE+00 AF125984.1 NT Aedes aegypti ucin-like protein MUC1 mRNA, complete cds 1364 7914 1.0 11.00E+00 X80416.1 NT V.carted Algal-CAM mRNA 1722 6845 12024 1.3 11.00IE+00 AB006531.1 NT Plautia stall intestine virus RNA for nonstructural polyprotein, capsid protein precursor, complete eds 1977 7092 0.5 11.00IE+00 P02704 SWISSPROT PROCOLIPASE A PRECURSOR 2435 7534--- 12740 0.5 1.0011E+00 P48355 SWISSPROT DNA GYRASE SUBUNIT B 2435 7534 12741 0.5 11.00IE+00 P48355 SWISSPROT DNA GYRASE SUBUNIT B 2651 7740 12940 1.1 1.OOE+00 AF131205.11 NT Mus musculus Serf l protein (Serf l), survival of motor neuron protein (Smn), neuronal apoptosis inhibitory protein-rs6 (Nalp-rs6), and neuronal apoptosis inhibitory protein-rs3 (Naip.

rs3) genes, complete cds 2846 7997 13093 3,5 1.OOE+00 P24008 SWiSSPROT 3-OXO-5-ALPHA-STEROID 4- DEHYDROGENASE 1 (STEROID 5-ALPHA-REDUCTASE 1) (SR TYPE 1) 2846 7997 13094 3.5 11.00E+00 P24008 SWISSPROT 3-OX0-5-ALPHA-STEROID 4- DEHYDROGENASE 1 (STEROID 5-ALPHA-REDUCTASE 1) (SR TYPE 1) 2938 8089 1.0 11.00E+00 014226 SWISSPROT HYPOTHETICAL 67.9 KID PROTEIN C61F12.08C IN CHROMOSOME 1 3162 8311 13423 1.2 1.OOE+00 AA628453.1 EST HUMAN af26g08.sl Soares_totaLfetus-Nb2HFI3. 9w Homo sapiens cDNA clone IMAGE:1 032830 X similar to WP:C42D8.3 CE04204;contains element MER22 MER22 repetitive element:

3277 8422 13530 1,9 1.0012+00 AF260827.1 NT lpornoea batatas cystelne protease mRNA, complete eds 3327 8471 0.8 11.00E+00 AF222761.1 NT Rattus norvegicus neuromedin U precursor (NmU) gene, exons 5 and 6 3548 5299 1.2 11.00E+00 U23808.11 NT Xenopus laevis rhodopsin gene, complete cds 3627 8766 13860 1.4 1.0012+00 AJ223816.1 NT Agadcus bl porus mRNA for tyrosinase 3683 8822 13915 1.3 1.OOE+00 U891198.1 NT Geococcyx velox cytochrome b (cytb) gene, mitochondrial gene encoding mitochondrial protein, complete cds 3683 8822 13916 1.3 1.OOE+00 U89198,1 NT Geococcyx velox cytochrome b (cytb) gene, mitochondrial gene encoding mitochondrial 3978 911 0.9 1.OOE+00 AF223391.1 NT protein, complete cds Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 4178 9304 0.8 1.OOE+00 8922245 NT Homo sapiens hypothetical protein FLJ10139 (FLJ10139), mRNA 4M 5.9 1.OOE+00 Q09472 SWISSPROT EIA-ASSOCIATED PROTEIN P300 9924 0,9 1.OOE+00 U757411.1 NT Taenia ovis 45W antigen (ToW4) gene. complete cds 4862 9969 15054 0.9 11.00E+00 AL1 63281.2 NT Homo saelens chromosome 21 segment HS21 C081 4951 10056 0.9 11.00IE+00 D10852.1 NT Rattus norvegicus mRNA for Nacetytglucosaminyitransferase Ill, complete cds 10234 15309 0.9 1.OOE+00 AJ223978.1 NT Bacillus subtilis 42.7kB DNA fragment from yvsA to yvqA 6680 11847 0.8 9.9OE-01 AF245455.1 NT -Drosophila melanogaster regulator of G-protel signalling LOCO Ill mRNA, complete cds j6 8-0 11848 0.8 9.90E-0 AF245455.1 NT Drosophila melanogaster regulator of G-protein signalling LOCO 111 mRNA, complete cds Page 7 of 196 Table 4

17 2598 7689 12892 0.9 9.9OE-01 AL163302.2 NT Homo sapiens chromosome 21 segment HS21C102 3553 8693 1.2 9.9OE-01 AF174585,1 NT Apple mosaic virus RNA 2 putative polymerase gene, complete cds 512 5676 10797 0.8 9.8OE-01 P22567 SWISSPROT AMINO-ACID ACETYLTRANSFERASE (WACETY1-GLUTAMATE SYNTHASE) (AGS) (NAGS) 5106 10202 1.0 9.70E-01 A1660384.1 EST HUMAN we62e04.xl Soares thymus NHIFTh Homo sapiens cDNA clone IMAGE:2345694 3' 2645 7734 12935 0.6 9.6OE-01 AJ400877,1 NT Homo sapiens ASCL3 gene, CEGP1 gene, Cl lorl14 gene, Cl lorl15 gene, Cl lorf16 gene and Cl 1 orfl 7 gene 4337 9460 14548 6.8 9.60E-01 AF197925.1 NT Bronlus inermis putative cytosolic phosphoglucomutase (pgml) mRNA, complete cds 4337 9460 14549 6.8 9.60E-01 AF197925.1 NT Bromus inermis putative cytosolic phosphoglucomutase (pgml) mRNA, complete cds 4355 9477 14568 1.3 9.60E-01 AW799674.1 EST HUMAN PM2-UM0053-240300-005- f12 UM0053 Homo sapiens cDNA 5083 10181 15261 0.9 9.6OE-01 7662375 NT Homo sapiens KIAA0914 gene product (K1AA0914), mRNA 3186 8335 13447 1.5 9.5OE-01 P22149 SWISSPROT IRON-REGULATED TRANSCRIPTIONAL REPRESSOR AFT1 1087 6225 11376 0.8 9.4012-01 6671685 NT Mus musculus catenin src (Catns), mRNA 1087 6225 11377 0.8 9.40E-01 6671685 NT Mus musculus catenin src (Catns), mRNA 3165 8314 3.4 9.40E-01 AF165990.1 NT Bartonelia clarridgeiae RNA polymerase beta subunit (rpoB) gene, partial cds 3181 8330 1.7 9.40E-01 AF080595.1 NT Pimpinelia brachycarpa zinc finger protein (ZFP1) mRNA, complete cds 1700 6823 1.1 9.3OE-01 AF242382.1 NT Homo sapiens phytanoyl-CoA hydroxylase (PHYH) gene, exon 5 2594 7686 12889 1.1 9.30E-01 BE071172.1 EST HUMAN RCS-BT0503-271 199-01 1- BO1 BT0503 Homo sapiens cDNA 3949 9082 14162 0.8 9.30E-01 M20219.1 NT Bovine papillomavirus type 2, complete genome 3949 9082 14163 0.8 9.30E-01 M20219.1 NT Bovine papillomavirus type 2, complete genome 4567 9685 14779 1.3 9.3OE-01 X06083.1 NT Calothrix cpcB3 and cpcA3 gene for phycocyanin 3 subunits beta and alpha 5155 10250 1.5 9.3OE-01 AF075615,1 NT Equus caballus microsatellite LEX013 3658 8797 13891 1.0 9.20E-01 Z74263.1 NT S.cerevislae chromosome IV reading frame ORF YD1-215c 2077 7189 0.5 9.1012-011 8923056 NT Homo sapiens hypothetical protein FLJ20048 (FLJ20048), mRNA 3169 8318 13430 1.0 9.1OE-01 T26418.1 EST-HUMAN AB20OG8R Infant brain, LLNL array of Dr. M. Soares 1 NIB Homo sapiens cDNA clone LLAB20OG8 5' 3169 8318 13431 1.0 9.1OE-01 T26418.1 EST-HUMAN AB20OG8R Infant brain, ILLNIL array of Dr. M. Soares I NIB Homo sapiens cDNA clone LLAB20OG8 5' 4310 9433 14523 0,9 9.1OE-011 U68172.1 NT Rattus norvegicus mucin (MUC2) gene, partial cds 1285 6415 11578 4.8 9.OOE-01 R02285.1 EST-HUMAN ye85c12.sl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE:124558 3' similar to gb:K03002 60S RIBOSOMAL PROTEIN L32 (HUMAN):

3170 8319 13432 0.9 9.OOE-01 7661625 NT Homo sapiens DKFZP564M2423 protein (DKFZP564M2423), mRNA 4282 9407 14491 2.4 9.OOE-01 AF099810.1 NT Homo sapiens neurexin Ill- alpha gene, partial cds 4919 10026 15104 1.5 9.OOE-01 U84903.1 NT Mus musculus L23 mitochondrial- related protein (L23mrp) gene, complete cds 4945 10050 15121 0.8 9.0OE-011 AF017729.1 NT Oryctol gus juniculus Rad51 (RAD51) mRNA, complete cds 1064 6202 11356 0.7 8.8OE-01 AE001358.1 NT Chlamydia trachomads section 85 of 87 of the complete genome 1064 6202 11357 0.7 8.8OE-01 AE001358.1 NT Chlarnydia trachomatis section 85 of 87 of the complete genome 3988 9120 14204 1.5 8.8012-01 AF079764.1 NT Drosophila melanogaster enhancer of polycomb (E(Pc)) mRNA, complete ods 4442 9562 14658 3.3 8.8OE-01 026350 SWISSPROT PUTATIVE F420-DEPENDENT NADP REDUCTASE 4 5623 10750 0.5 8.70E-01 AF1 06953.2 NT Homo sapiens SOS1 (SOS1) gene, partial cds --- 12662 1.1 8.70E-01 5901893 NT AHomo sapiens AT-binding transcription factor 1 (ATBF1), mRNA 27 7455 1 Page 8 of 196 Table 4

2845 7996 13092 4.6 8.70E-01 AA595863,11 EST HUMAN nnO5fl 1.si NCI CGAP Pr41 Horno sapiens eDNA done IMAGE:1 076877 4938 10044 2.5 8.70E-01 AF121970.1 NT Pseudomonas aeruginosa topoisomerase (top), putative transcriptional regulatory protein OhbR (ohbR), ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA (ohbA), OhbC (ohbC), ortho-halobenzoate 11,2-dioxygenase alpha-ISP protein 0MB (ohbB), and put> 466 5631 0.6 8.60E-01 X1 7012.1 NT Rat lGFlI gene for Insulin-like growth factor 11 840 5989 11150 1.2 8.60E-01 W69089.11 EST HUMAN zd44e03xl Soares-fetal- hearLNbHH19W Homo sapiens cDNA clone IMAGE:343516 5' 2224 7331 12544 1.0 8.60E-01 4503210 NT Homo sapiens cytochrome P450, subfamily XWIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1 (CYP27A1 b) mRNA 3568 8707 13805 0.8 8.60E-01 AL1611565.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 65 3730 8867 13959 1,3 8.60E-01 U49724.1 NT Drosophila melanogaster merlin (Dmedin) mIRNA, complete cels 5046 10146 15224 1.0 8.6OE-01 AE002239.2 NT Chlarnydophila pneumoniae AR39, section 67 of 94 of the complete genome 3930 9064 14151 3.1 8.50E-01 A1215419.1 EST-HUMAN qhl 1b01.xl Soares_INIFL T GBC S1 Homo sapiens cDNA done IMAGE:1844329 3' 2006 7120 12321 1.1 8.40E-01 AA0781112.1 EST HUMAN 7H14C04 Chromosome 7 HeLa cDNA Library Homo saplens cDNA clone 7H14C04 4108 9234 14317 0.8 8.40E-01 AF143509.1 NT Mus musculus NK cell receptor 2B4 gene, promoter region and partial cds 5045 10145 15223 1.0 8.40E-01 AA078112.1 EST HUMAN 7H14C04 Chromosome 7 HeLa cDNA Library Homo sapiens cDNA clone 7H14C04 724 5878 11018 0.8 8.30E-01 M93437.1 NT Thermus thermophilus cytochrome c- 552 (cycA) and CycB (cycB) genes, complete cds 3056 8207 13306 2.6 8.30E-01 AL161506.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 18 3739 8876 13966 1.0 8.30E-01 AB010879.1 NT Nicotlana tabacum mIRNA for'chloroplast ribosomal protein L1 0, complete cds 4628 9742 1,9 8.30E-01 AF083221.11 NT Fugu rubripes putative neurotransmitter receptors, YDR140w homolog, and glycinamide dbonucleotide transformylase (GART) genes, complete cds 4917 10024 15102 1.1 8.30E-01 U469116.1 NT Canis familiaris MI-IC DLA Class 11 DRB pseudogene DR132 4917 10024 15103 1.1 8.30E-01 U46916.1 NT Canis famillarls WC DLA Class 11 DRB pseudogene DR132 5177 10024 15102 1.0 8.3OE-01 U46916.1 NT Canis familiarls MI-IC DLA Class 11 DRB pseudogene DRP 5177 10024 15103 1.0 8.30E-01 U46916.1 NT Canis famillaris MI-IC DLA Class 11 DRB pseudogene DRB2 2043 7156 0.5 8.20E-01 AF145589.1 NT Mus musculus trophinin (Tnn) gene, complete cds 2646 7735 1.1 8.20E-01 AW376990.1 ESTHUMAN IL3-CTO2109-161199-031-CO8 CT0219 Homo sapiens ONA 4308 9431 14520 1.2 8.20E-01 7662295 NT Homo sapiens KIAA0769 gene product (K1AA0769), mIRNA 2731 7815 1.3 8.1OE-01 AF191839.1 NT Mus musculus TANK binding kinase TIE1K1 (Tbkl) mRNA, complete cds 3412 8554 13662 2,8 8.1OE-01 AF055066.1 NT Homo sapiens WC class 1 region 3412 8554 13663 2.8 8.1OE-011 AF055066.11 NT Homo sapiens WC class 1 region 5364 1.0 8.OOE-01 AJ271510.1 NT Staphylococcus aureus partial pta gene for phosphate actylltransferase allele 15 285 5472 10602 1.3 8.0OE-011 AJ132772.1 NT Bos taurus futh and rtif genes 763 5916 11063 1.1 8.0OE-011 AF208153.1 NT Drosophila americana texana strain LP97.09 transformer (tra) gene, tra-D-am.01 allele, partial cds 1603 6730 11902 1.2 8.OOE-01 Z541 99.1 NT Lesculenturn DNA Allsa cralq encoding 1 -aminocyclopropane-l -carboxylic add oxidase 3039 8191 13293 2.7 8.0OE-011 AF127897.11 NT Salmid boliviensis olfactory receptor (SB027) gene, partial cds 1=3 8418 1352.1 8.0OE-011 ABOO6193.1 NT Mus musculus gene for oviductal glycoprotein, complete cds Page 9 of 196 Table 4

3643 8782 1.8 8.OOE-01 AL162758.2 NT Neisseria meningiddis serogroup A strain Z2491 complete genome; segment 717 4432 9553 14647 5.7 8.OOE-01 X83739.2 NT G.gallus mRNA for nicotinic acetylcholine receptor (nAChR) beta 3 subunit 446 5612 10743 1.3 7.9OE-01 D1 1476.1 NT Lymantda dispar nuclear polyhedrosis virus gene for DNA polymerase, complete cds 698 5853 1.2 7.9OE-01 AE0021130.1 NT Ureaplasma urealyticum section 31 of 59 of the complete genome 1587 6713 66.0 7.9OE-01 A8040885.1 NT Homo saplens mRNA for KIAA1452 protein, partial ods 1634 6760 1.4 7.9012-01 U32739.1 NT Haemophilus Influenzae Rd section 54 of 16,3 of the complete genome 2216 7324 12538 1.9 7.9OE-01 AB004816.1 NT Oryctolagus cuniculus mRNA for mitsugum!n29, complete cds 2217 7325 12539 0.8 7.9012-01 AF130459.1 NT Danio rerio Trp4-associated protein TapIA (taplA) mRNA, complete cds 3472 8613 13725 2.8 7.9OE-01 AF228664.1 NT Gallus gallus SOX8 transcription factor (SOX8) mRNA, complete cds 4204 9329 0.9 7.9012-01 BE263612.1 EST-HUMAN 601192033F1 N11-1_MGC 7 Homo sapiens cDNA done IMGE:3535785 5, 4508 9627 14720 1.0 7.9OE-01 6753745 NT Mus musculus embigin (Emb), mRNA 4508 9627 14721 1.0 7.9OE-01 6753745 NT Mus musculus embigin (Emb), mRNA 5075 10173 15252 0.8 7.9OE-01 Z4721 0,1 NT S.pneumoniae dexB, cap3A, cap3B and cap3C genes and orts 5075 10173 15253 0.8 7.9OE-01 Z4721 0.1 NT S.pneumonlae dexB, cap3A, cap3B and cap3C genes and orfs 5084 10182 1.0 7.9OE-01 M29930.1 NT Human Insulin receptor (allele 2) gene, exons 14, 15, 16 and 17 858 6006 0.6 7.8OE-01 Z43785.1 EST-HUMAN HSCI KH041 normalized infant brain cIDNA Homo saplens eDNA clone c-1 kh04 2229 7336 12548 1.2 7.8OE-01 AW959567.1 EST HUMAN EST371637 MAGE resequences. MAGF Homo sapiens cIDNA 4603 9717 14810 0.8 7.8OE-01 U87305.1 NT Rattus norvegicus transmembrane receptor Unc51-11 mRNA, complete cds 138 5334 10464 2.2 7.7OE-01 AF184345.1 NT Lycopersicon hirsutum ADIP-. glucose pyrophosphorylase large subunit (AGP-Ll) mRNA, complete cds 709 5863 0.6 7.7OE-01 AF050157.1 NT Mus musculus major histocompatibility locus class 11 region: major histocompatibility protein class 11 alpha chain (1Aalpha) and major histocompatibility protein class 11 beta chain (]Ebeta) genes, complete ods; butyrophilin-like (NG9), butyrophilin-li> 772 5924 11073 0.7 7.7OE-01 P02921 SWISSPROT MELIBIOSE CARRIER PROTEIN (THIOMETHYLGALACTOSIL)F- PERMEASE 11) (MELIBIOSE PERMEASE) (NA+ (L1+)/MELIBIOSE SYMPORTER) (MELIBIOSE TRANSPORTER1 2680 7767 12968 1.0 7.7OE-01 033915 SWISSPROT CITRATE SYNTHASE 3313 8458 0,8 7.7OE-01 8393408 NT Homo sapiens UDP-N-acetyl-alpha-D- galactosamine:polypepdde N acetyigalactosaminyitransferase 7 (GaINAc-T7) (GAI-NAC-T7), mRNA 3549 8689 13791 4.5 7.7OE-01 AF1 18085.1 NT Homo sapiens PRO1975 mRNA,. complete ods 3722 8859 2.3 7.7OE-01 6753425 NT Mus musculus cytokine inducible SH2containing protein (Cish), mRNA 4297 9421 14511 2.9 7.7OE-01 AF199488.1 NT Cotumix cotumix japonica sub- species japonica beta-actin mRNA, partial cds 4297 9421 14512 2.9 7.7OE-01 AF199488.1 NT Cotumix cotumix japonica sub- species japonica beta-actin mRNA, partial eds 502 5666 0.5 7.5OE-01 AL163301.2 NT Homo sapiens chromosome 21 segment HS21 Cl 01 569 5730 10847 0.8 7.5OE-01 AF020503.1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 1972 7087 0.9 7.5OE-01 4505834 NT Homo sapiens polycysfic kidney disease 2 (autosomal dominant) -NOTE: redefinition of symbol (PM2) mRNA 3315 8460 13572 0.9 7.5OE-01 C14203.1 EST HUMAN C14203 Clontech human aorta polyA+ mRNA (#6572) Homo saplens cDNA clone GEN 03 115' 457--- 5305 10432 13.9 7.5OE-01 8922672 NT Homo sapiens hypothetical protein FLJ10793 (FU10793), mRNA Page 10 of 196.

1 Table 4

1114 6251 11401 1.2 7.4OE-01 A1598146,1 EST-HUMAN tn14b09.xl NCI_PGAP_Bm25 Homo sapiens cDNA clone IMAGE:2167577 Ysimilar to contains Alu repetitive element;contalns element MIR repetitive element; 2295 7399 12608 1.0 7.4012-01 AB01 1106.1 NT Homo sapiens mRNA for KIAA0534 protein, partial cds 3665 8804 13898 1.2 7.4OE-01 AF1 12538.1 NT Malva pusilla actin (Actl) mRNA, complete cds 4213 9337 14421 4.4 7.4012-01 AL.163246.2 NT Homo saplens chromosome 21 segment HS21C046 4602 9716 14809 4.8 7.4OE-01 BE094286.1 EST-HUMAN PM3-BT0764-130500-001- c03 BT0764 Hoffio saplens cDNA 4515 9634 14726 1.0 7.3OE-01 AE001 166.1 NT Borrelia burgdorfed (section 52 of 70) of the complete genome 5127 10222 1.1 7.3OE-01 5360211 NT Homo sapiens growth affest-specific 7 (GAS7), transcript variant b, mRNA 814 5964 0.9 7.2OE-01 L29281.1 NT Rattus norvegicus initiation factor-2 kinase (eIF-2a) mRNA, complete cds 1914 7031 12219 1.3 7.2OE-01 X79140.1 NT Ktabacurn Ne1F4A13 mRNA 2412 7511 12718 0.5 7.2OE-01 A13009605.1 NT Gallus gallus gene for melanocortin 2-receptor, complete cds 3028 8180 13280 1.2 7.2OE-01 AF198100.1 NT Fowlpox virus, complete genome 3406 8548 13656 2.1 7.2OE-01 AF065606.1 NT Giardia intestinalls variant- specific surface protein (vsp417-6) gene, vsp417-61A-1 allele.

complete cds 3736 8873 5.1 7.2OE-01 A13010810.1 NT Candida albicans gene for phospholipase D, complete cds 4660 9772 14867 2.4 7.2OE-01 D90314,1 NT L.mesenteroldes gene for sucrose phosphorylase (EC 2.4.1.7) 5064 10162 15238 1.2 7.2012-01 AF196779.1 NT Homo sapiens transcription factor IGHM enhancer 3, JM1 1 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentlation-dependent protein, triple LIM domain protein 6, and synaptophysin genes,'complete ods; and L-type calcium channel a> 5064 101621 15239 1.2 7.2OE-01 AF196779.1 NT Homo sapiens transcriptionfactor IGHM enhancer 3, JM1 1 protein, JM4 protein, JM5 protein, T54 protein, JMIO protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, complete cds; and L-type calcium channel a> 677 5832 10963 3,5 7.1OE-01 D21070.1 NT Rana catesbelana mRNA for bullfrog skeletal muscle calcium release channel (ryanodine receptor) alpha Isoform(Ry1R1), complete cds 3024 8176 13277 10.8 7.1OE-01 AJ270777.1 NT Homo sapiens partial TCF-4 gene for T-cell transcription factor-4, exons 15-16 4114 9240 14325 2.9 7.1OE-01 7305360 NT Mus musculus otogelin (Otog), mRNA 4114 9240 14326 2.9 7.1OE-01 7305360 NT Mus musculus otogelin (Otog), mRNA 4688 9800 14903 0.8 7.1OE-01 Z73277.1 NT S.cerevislae chromosome X11 reading frame ORF YLIR105c 1209 6340 11498 1.0 7.OOE-01 AB014514.1 NT Homo'sapiens mRNA for KIAA0614 protein, partial cds 1209 6340 11499 1.0.7.OOE-01 A13014514.1 NT Homo sapiens mRNA for KIAA0614 protein, partial cds 2399 7499 12705 0,5 7.OOE-01 N62412.1 EST-HUMAN yz73e07.sl Soares_mulfiple-scierosis-2NbHMSP Homo saplens cIDNA clone IMAGE:288708 Ysimilar to contains Alu repetitive element; 2399 7499 12706 0,5 7.0OE-011 N62412.1 EST HUMAN yz73e07.sl Soares_multiple - scierosis_2NbHMSP Homo sapiens cDNA clone JMAGE:288708 Ysimilar to contains AW repetitive element; 4989 10092 2.0 7.OOE-01 AL.163301.2 NT Homo saplens chromosome 21 segment HS21C1 01 954 6100 11257 5.4 6.9OE-01 U69674.1 NT Candida albicans squalene epoyJdase (CAERG1) gene, complete cds and translational regulator gene, partial ods 9 '. 6100 11258 5.4 6.9OE-01 U69674.1 NT Candida albicans squalene opoyJdase (CAERG1) gene, complete cds and translational regulator gene, partial cds Page 11 of 196 Table 4

1288 6418 11581 0.8 6.9OE-01 AA593530.1 EST HUMAN rin28a09.sl NCI_WAP Gasl Homo sapiens cDNA clone IMAGE:1 085176 3' 3182 8331 13443 1.6 6.9OE-01 AE002271.2 NT Chlamydia uridarum, section 3 of 85 of the complete genome 3341 8485 13599 2.6 6.9OE-01 6677734 NT Mus musculus ral quanine nucleotide dissociation stimulator (Rgds), mRNA Mus musculus mRNA for Immunoglobulin gamma heavy chain variable region, isolate PC 3399 8541. 13649 0.9 6.9OE-01 Y17373.1 NT 2811 941 6088 11245 1.0 6.8012-01 AF017784.1 NT Giardia infesfinalis carbamate kinase gene, complete cds aj75a05.sl Soares_parathyroid tumor NbHPA Homo sapiens cDNA clone IMAGE:1402256 Ysimilar to 9M5641 1-mal AiCOHOiL DEHYDROGENASE CLASS 11 PI CHAIN (HUMAN). , 2799 6724 11894 0.6 6.8012-01 AA854475.1 EST-HUMAN 4469 9589 14685 1,3 6.8OE-01 J00762.1 NT Rat(hooded) prolactin gene: exon Ill and flanks 4749 9860 14952 0.8 6.8OE-01 4758521 NT Homo sapiens hevin (HEVIN) mRNA Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B- cells 1 (NFKB1) 295 5481 10612 10.0 6.7OE-01 AF213884.1 NT gene, complete cds Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B- cells 1 (NFKB1) 336 5517 10642 6,7 6.7OE-01 AF213884.1 NT gene, complete cds W2912.sl Soares - totaLfetus-Nb2HF_gw Homo saplens cDNA clone IMAGE:786310 3' 2100 7211 12419 0.6 6.7OE-01 AA451864.1 EST HUMAN similar to contains element TAR1 repetitive element; Drosophila melanogaster Mst85C gene, complete cds; NIVIDIVIC isoform (Nmdmc) gene, complete cds, altemafively spliced; and transcription factor (Relish) gene, complete cds, 2120 7935 12440 1.3 6.70E-01 AF186073.1 NT altemabvely spliced 2966 8117 13224 3.7 6.7OE-01 6678580 NT Mus musculus Wiskott-Aldrich syndrome rotein (Wasp), mRNA 4350 9472 14564 1.0 6.70E-01 X74421.1 NT S.tuberosum mRNA for glucose-6- phosphate dehydrogenase 4881 9989 15070 0,9 6.7OE-01 AW0791 10.1 ' EST HUMAN xa95912.xl NCI_PGAP Col 7 Horno, sapiens cDNA done IMAGE:2574598 3' 5182 10274 15343 0.9 6.7OE-01 AJ252942.1 NT Dendrobium firribriaturn mRNA for phosphoenolpyruvate carboxylase, partial 2452 7551 12759 0.9 6.6OE-01 AF075240.1 NT Homo saplens SLIT1 protein ( LIL2) mRNA, partial cds Homo sapiens lens epithefium-derived growth factor gene, alternatively spliced, complete cds 2667 7754 12954 1.3 6.6OE-01 AF199339.1 NT Homo saplens sema domain, seven thrombospondin repeats (type 1 and type 1 -like), transmembrane domain (TM) and short cytoplasmic domain, (sernaphorin) 5A (SEIVIA5A) 3445 8587 13700 1.0 6.6OE-01 4506880 NT mRNA 3484 8625 13737 1,1 6.6OE-01 AB01 1536.1 NT Horno sapiens mRNA for MEW2, partial cds 3484 8625 13738 -1. 1 6.6OE-01 AB01 1536.1 NT Homo sapiens mRNA for MEGF2, partial cds 3608 8747 13842 3.1 6.6OE-01 Y07669.1 NT C.albicans random DNA marker, 282bp Human hereditary haemochr.omatosis region, histone 2A-like protein gene, hereditary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) 4020 9149 0.9 6.6OE-01 U91328.1 NT gene, complete cds 608 5766 10884 1.4 6.5OE-01 M75140.1 NT H.vulgads Na,K-ATPase alpha subunit mRNA, complete cds 608 5766 10885 1.4 6.50E-01 M75140.1 NT H.vulgads N K-ATPase alpha subunit mRNA, complete cds 3386 8529 13636 5.1 6.5OE-01 AB041225.1 NT Mus musculus gene for Tob2, complete cds 3951 9084 14165 1.1 6.5OE-01.4504632 NT Homo saplens intedeukin 10 receptor, alpha (IL1ORA) mRNA = '- 93-11 14392 4.2 6.5OE-01 AJ272265.1 NT Horno sapiens SPP2 gene for secreted phosphoprotein 24 precursor, exons 1-8 1 1 Page 12 of 196 Table 4

4215 9339 14422 0.9 6.5OE-01 AL161539.2 NT Arabldopsis thaliana DNA chromosome 4, contig fragment No. 39 4994 10097 15164 2.3 6.5OE-01 U28921.1 NT Phaseolus vulgaris ATPase gamma subunit mRNA, nuclear gene encoding mitochondrial protein, partial cds 5031 10131 15207 1.0 6.5OE-01 4826799 NT Homo sapiens potassium inwardly- rectifying channel, subfamily J, member 5 (KCNJ5) mRNA 5175 10269 0.9 6.5OE-01 U37258.11 NT Acetobacter xylinum putative ATP binding protein delta-AceB gene, partial cds, and GDP mannose:celloblosyl-diphosphopolyprenoI alpha-mannosyttransferse gene, complete ods 252 5440 10567 1.8 6.4OE-01 U48848.1 NT Drosophila melanogaster 8W dynein fight chain mRNA, complete cds 3410 8552 13660 2.5 6.4OE-01- U48854.2 NT Mus musculus dystroglycan 1 (DAG1) 9Lne, exons 1 and 2 and complete cds 4389 9511 14598 0.8 6.4OE-01 Y12488.1 NT M.musculus whn gene 4389 9511 14599 0.8 6.40E-01 Y12488.1 NT M.musculus whn gene 427.5594 10728 1.5 6.3OE-01 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFI-IRP-111) 524 5688 10809 0.7 6.3OE-01 U32689.11 NT Haemophilus influenzae Rd section 4 of 163 of the complete genome 1505 6631 11800 1.5 6.3OE-01 AA365227.1 EST-HUMAN EST75935 Pineal gland 11 Homo saplens cIDNA Vend similar to similar to orthodentide related pro n 1 2116 7227 12435 1.4 6.3OE-01 U81 136.1 NT Shi ella flexned mulfi-andblobc resistance locus 2534 7630 12832 1.4 6.3OE-01 U753311.1 NT Gallus gallus bone morphogenedc protein 1 (BMP1) mRNA, partial cds 2534 7630 12833 1.4 6.3OE-01 U75331.1 NT Gallus gallus bone morphogenedc protein 1 (13MP1) mRNA, partial cds 2983 8135 0.9 6.3OE-01 Y17275.1 NT Lycopersicon esculentum p69a gene. complete CDS 3985 9117 14200 1,4 6.3OE-01 X99675.1 NT D.melanogaster mRNA for metabotropic glutamate receptor 4755 9866 14959 1.5 6.2OE-01 4504402 NT Homo sapiens major histocompatibility complex, class 11, DO beta (HLA-DOB) mRNA 5002 10105 15173 1.0 6.20E-01 A1587039.1 EST HUMAN tr55d08.xl NCI- CGAP_Panl Homo sapiens cIDNA done IMAGE:2222223 Ysimilar to contains element MSR1 repetitive element; 2345 7447 1.6 6.1OE-01 6678076 NT Mus musculus secreted acidic Systelne rich glycoprotein.(Sparc), mRNA 2956 8107 1.3 6.1OE-011 AF153704.1 NT Tetrahyme a thermophila dyncin heavy chain (DYH8) gene, partial cds 4931 10037 15109 1.0 6.1 OE-01 L20427.1 NT Rattus norvegicus dihydroxypolyprenyibenzoate methyltraferase mRNA, complete cds 4931 10037 15110 1.0 6.1012-01 1-20427.1 NT Rattus norvegicus dihydroxypolyprenyfbenzoate methyltransferase mRNA, complete cds 486 5651 10775 0.9 6.OOE-01 D87675.1 NT Homo sa ns DNA for arnylold precursor protein, complete cds 549 5712 0.9 6.0OE-01 5802999 NT Homo sapiens adaptor-related protein complex 3, mu 2 subunit (CLA20), mRNA 1339 6467 11635 0,6 6.OOE-01 AF065253.1 NT Human respiratory syncytial virus strain CH93.53b attachment protein (G) gene, complete eds 3744 8881 13970 1.0 6.0OE-011 AJ233396.1 NT Viral hemorrhagic septicemia virus N, P, M, G, Nv, L genes, French strain 07-71 984 6129 11284 1.3 5.9OE-01 U32701.11 NT Haemophilus Influenzae Rd section 16 of 163 of the complete genome 2354 7456 12663 2.3 5.9OE-01 A1859996.1 EST-HUMAN wm22f01.xl NCI CGAP U14 Horno sapiens cDNA clone IMAGE:2436697 3, 3232 8380 13486 4,9 5.9OE-01 AL163267.2 NT Homo_sapi ns chromosome 21 segment HS21 C067 3232 8380 13487 4.9 5.9OE-01 AL163267.2 NT Horno saplens chromosome 21 segment HS21C067 41 9255 4.1 5.9OE-01 AF162756.1 NT Rattus norvegicus ceneAn 2 mRNA, partial ods 1W 6985 12169 0.5 5.8OE-01 P40472 SWiSSPROT SIMI PROTEIN Page 13 of 196 1 Table 4

3401 8543 13651 2.0 5.8OE-01 AF154923.1 NT Mycoplasma arthriddis strain H39 putative cldhesin MAA1 (maal) gene, complete cds 3900 9035 14127 1.0 5.8OE-01 7662059 NT Homo sapiens KIAA0335 gene product (K1AA0335), mRNA 4413 9533 14,622 2.8 5.8OE-01 A13009077.1 NT Viona radiata mRNA for proton pyrophosphatase, complete cds 4690 9802 1.0 5.8OE-01 AF1 10846,1 NT Megaselia scalaris sex-lethal homolog (Megsxi) gene, partial ods, alternatively spliced products 764 5917 11064 2.0 5.70E-01 D83536.2 NT Escherichia cofi genomic DNA, (4. 1 - 6.1 min) 1840 7928 12144 0.9 5.70E-01 A13024336.11 NT Mus musculus mRNA for membrane-boundtransferrin-like protein p97, complete cds 1840 7928 12145 0.9 5.70E-01 A13024336.1 NT Mus musculus mRNA for membrane-bound transfenin-like protein p97, complete cels 3460 8601 2,4 5.7OE-01 A13033503.1 NT Populus euramericana peacs-2 mRNA for 1-aminocycJopropane-l-carboxylate synthase, complete cds 3829 8966 14065 1.7 5.7OE-01 AF01 1581.1 NT Horno sapiens T cell receptoFbeta chain (13V6S72-13.1 1 Sli mRNA, partial cds 5201 10292 15359 2.2 5.7OE-01 U78517.1 NT Rattus norvegicus cAMP- regulated guanine nucleotilde exchange factor 11 (cAMP-GEFII) mRNA, partial cds 3318 8463 13575 1.2 5.6OE-01 A13018283.2 NT Homo sapiens mRNA for KIAA0740 protein, partial cds 3318 8463 13576 1.2 5.60E-01 AB018283.2 NT Homo sapiens mRNA for KIAA0740 protein, partial ods 3808 8945 14041 0.8 5.60E-01 A1161501.2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 13 4146 9272 14350 0.8 5.6OE-01 D831135.1 NT Chicken TBP gene, exon8, complete cds 4726 9838 14930 0.8 5.6OE-01 7330334 NT Homo sapi ns chloride intracellular channel 4 like (CLIC4L), mRNA 1193 6325 11481 1.1 5.5012-01 8393912 NT Rattus norvegicus Propionyl Coenzyme A carboxylase, beta polypepfide (Pccb), mRNA 2668 7755 12955 1.0 5.5OE-01 P03341 SWISSPROT GAG POLYPROTEIN [CONTAINS: INNER COAT PROTEIN P12, CORE PROTEIN P15; CORE SHELL PROTEIN P30; NUCLEOPROTEIN P10] 2668 7755 12956 1.0 5.5OE-01 P03341 SWISSPROT GAG POLYPROTEIN [CONTAINS: INNER COAT PROTEIN P12; CORE PROTEIN P15; CORE SHELL PROTEIN P30; NUCLE6PROTEIN P101 2888 8039 13140 1.3 5.5OE-01 5902085 NT Homo sapiens superkiller viralicidic activ4 2 (S. cerevislae homolog)-iike (SKIV21---),mRNA 3027 8179 1.4 5.50E-01 H4621 9.1 EST HUMAN yol 8al U1 Soares adult brain N2b51-112155Y Homo sapiens cDNA done 1 MAGE: 178266 3' 3196 8345 13458 3.1 5.50E-01 AF227240.11 NT Rabbit oral papillomavirus, complete genome 3634 8773 13867 1.3 5.50E-01 P48755 SWISSPROT FOS-RELATED ANTIGEN-1 139 5335 10465 1.3 5.4OE-01 7657266 NT Homo sapiens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog (K1AA0929), mRNA 139 5335 10466 1.3 5.40E-01 7657266 NT Homo sapiens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog (K1AA0929), mRNA 570 5731 10848 0.9 5.40E-01 AF232006.1 NT Pseudomonas syringae pv. tomato strain DC3000 AvrE (avrE), HrpW (hrpW), and GstA (gstA) genes, complete eds; and unknown genes 570 5731 10849 0.9 5.4OE-01 AF232006.1 NT Pseudomonas syringae pv. tomato strain DC3000 AvrE (avrE), HrpW (hrpW), and GstA (gstA) genes, complete cds; and unknown genes 1250 6379 11538 0.8 5.4OE-01 AW896087.1 EST_HUMAN QV4-NN0040-070400-160- c04 NNO040 Homo sapiens cDNA 2059 7171 12530 1.0 5.40E-01 AE002247.2 NT Chlarnydophila pneumoniae AR39, section 74 of 94 of the complete genome 2208 7317 0.8 5.40E-01 AJ276682.1 NT Drosophila melanogaster mRNA for 15, 15' beta carotene dioxygenase (beta-diox gene) Page 14 of 196 1 Table 4

505 5669 10792 0.7 5.3OE-01 AF019413.1 NT Homo sapiens HLA class Ill region containing tenascin X (tenasdn-X) gene, partial cds:

cytochrome P450 21-hydroxylase (CYP21B), complement component C4 (C413) G1 1, helicase (SK12W), RD, complement factor B (B1). and complement component C2 (C2) genes,> 2093 7204. 12409 0.9 5.3OE-01 AF113919.1 NT Brassica oleracea var. capita phospholipase D2 (PLD2) gene, complete cds 2093 7204 12410 0.9 5.30E-01 AF113919.1 NT Brassica oleracea var. capitata phosph lipase D2 (PLD2) gene, complete eds 2332 7435 12643 0.9 5.3OE-01 4506210 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 3 (PSMC3) mRNA 2755 7839 13040 3.0 5.3OE-01 4506328 NT Homo sapiens protein tyrosine phosphatase, receptor-type, zeta polypepfide 1 (PTIPRZII) mRNA 2755 7839 13041 3.0 5.3OE-01 4506328 NT Homo sapiens protein tyrosine phosphatase, receptor-type, zeta polypeptide 1 (PTIPRZI) mRNA 3205 8353 13463 2.9 5.3OE-01 AF087658.1 NT Homo sapiens secreted C-type lectin precursor (I-SI-CL) gene, complete cds 3919 9053 2,6 5.3OE-01 M62977.1 NT Xenopus laevis LDL receptor-1 gene, W flank 4117 9243 1.3 5.3OE-01 U39687.1 NT Mycoplasma genitallum section 9 of 51 of the complete genome 799 5950 11100 3.9 5.20E-01 L20770.1 NT Drosophila melanogaster helix- loop-hefix mRNA, complete ods 1148 6283 11436 2.6 5.2OE-01 Q9VVV30 SWISSPROT NUCLEAR FACTOR OF ACTIVATED T CELLS 5 IT CELL TRANSCRIPTION FACTOR NFAT5) (NF-AT5) (REL DOMAIN-CONTAINING TRANSCRIPTION FACTOR NFATS) 1174 6308 11462 1.0 5.2OE-01 AF224492.1 NT Homo sapiens phospholipid s mblase 1 gene, complete ods 1843 6961 1.8 5.2OE-01 A1.163285.2 NT Homo sapiens chromosome 21 segment HS21C085 2099 7210 12418 1.0 5,20E-01 A13018283.2 NT Homo sapiens mRNA for KIAA0740 protein, partial cds 3081 8232 13330 1.9 5.2OE-01 U65942.1 NT Chlarnydophila abortus strain S2613 POMP91A and POMP90A precursor, genes, complete cds 3199 8348 1.2 5.20E-01 D73443.1 NT Azotobacter vinelandii Icd gene for Isocitrate dehydrogenase, complete cds 3359 8502 1.8 5.20E-01 AL1 16780.1 NT Botr fl yfis cinerea strain T4 eDNA library under conditions of nitrogen dep vation 3397 8539 13646 1.. 9 5.2012-01 AA984165.1 EST HUMAN am77g05.sli Stratagene schlio.brain S1 1 Homo sapiens cDNA clone IMAGE:1 616504 3' 3580 8719 1.1 5.2OE-01 AF020269.1 NT Medicago sadva chloroplast malate dehydrogenase precursor (pl mdh) mRNA, nuclear gene encoding chloroplast protein, complete cds 3582 8721 13819 1.1 5.2OE-01 U82671.2 NT Homo sapiens chromosome Xq28 melanoma andgen family A2a (MAGEA2A), melanorna antigen family A12 (MAGEA12), melanoma antigen family A2b (MAGEA2B), melanoma antigen family A3 (MAGEA3), caltractin (CALT), NAD(P)H dehydrogenase-like protein (NSDHL), and Li> 4949 10054 1.3 5.2OE-01 7106444 NT Mus musculus vaniliold receptor-like protein 1 (VrIl), mRNA 601 5759 10879 0.6 5.1OE-01 M58509.1 NT Human adrenodo)dn reductase gene, exons 3 to 12 632 5791 10913 1.1 5.1OE-01 A.1233944.1 NT Polyangium vitellinum (strain PI vtl) 16S rRNA gene 632 5791 10914 1.1 5.1OE-01 A.1233944.1 NT Polyanglum vitellinum (strain PI vtl) 16S rRNA gene 16 6758 1.1 5.1OE-01 X87885.1 NT R.norvegicus mIRNA for mammalian fusca protein 3984 9116 14199 3.8 5.1OE-01 A1858495.1 xl NCI CGAP Utl Horno saplens cDNA done IMAGE:2427263 3' F-409L41 9220 14303 2.9 5.1OE-01 P96380 SWISSPROT TRANSCRIPTION-REPAIR COUPLING FACTOR (TRCF) F-; 7199 12403 1.4 5.OOE-01 1 48855J52 NT Homo sapiens postmelodc segregation increa d 2-like 9 (PMS2L9), mRNA Page 15 of 196 Table 4

1-9 C) 2087 7199 12404 1.4 5.OOE-01 4885552 NT Horno sapiens postmelofic segregation increased 2-like 9 (PMS2L9), MRNA 2097 7208 12414 1.3 5.00E-01 AF008210.1 NT Buchnera aphidicola genomic fragment containing (chaperone Hsp60) groEL, DNA biosynthesis initiating protein (dnaA), ATP operon (atpCDGAHFEB), and putative chromosome replication protein (gidA) genes, complete cds; and termination factor Rho (rho) gene> 2097 7208 12415 1.3 5.OOE-01 AF008210.1 NT Buchnera aphidicola genomic fragment containing (chaperone Hsp60) groEL, DNA blosynthesis initiating protein (dnaA), ATP operon (atpCDGAHFEB), and putative chromosome replication protein (gidA) genes, complete cds; and termination factor Rho (rho) gene> 2543 7639 12839 0.9 5.OOE-01 U50730.2 NT Human cyclin-dependent kinase 2 (cdk2) gene, 5' promoter region 2543 7639 12840 0.9 5.OOE-01 U50730.2 NT Human cyclin-dependent kinase 2 (cdk2) gene, 5' promoter region 3688 8826 13920 0.8 5.OOE-01 U55574.1 NT Mus musculus and-DNA Immunoglobulin light chain lgM mRNA, antibody 363p. 138, partial cds 3797 8934 14027 3.4 5.OOE-01 AB03301 0.1 NT Homo sapiens mRNA for K1AA11 184 protein, partial cds 1864 6982 12166 1.4 4.9012-01 U40869.1 NT Cavia porcellus pulmonary surfactant protein A (SP-a) mRNA, complete cds 2111 7222 12431 1,1 4.8OE-01 L01652,1 NT Human ventricular myasin light chain 2 gene, seven exons 4577 9362 1.1 4.8012-01 4504850 NT Homo sapiens potassium channel, subfamily K, member 5 (TASK-2) (KCNK5) mRNA, and translated products 1950 7067 12263 0.8 4.7OE-01 X76536.1 NT S.tuberosurn L. (Desiree) PHA1 mRNA 3674 8813 13907 1.3 4.6012-01 AW818638.1 EST-HUMAN RC1-ST0278-040400-018- b06 ST0278 Horno sapiens cDNA 5087 10185 1.0 4.6OE-01 M1 1267.1 NT Bovine steroid 21-hydroxylase gene (P-450-c21) gene, complete eds 965 6110 11266 1.0 4.5OE-01 AA249416.1 EST-HUMAN -13550.seq.F Human fetal heart, Lambda ZAP Express Homo saplens cDNA 5' 1847 6965 12150 1.3 4.5OE-01 AB033078.1 NT Homo sapiens mRNA for KIAA1252 protein, partial cds 847 6965 12151 1.3 4.50E-01 AB033078.1 NT Homo sapiens mRNA for KIAA1252 protein, partial cds 1869 6987 12171 0.6 4.5OE-01 AE001931.1 NT Deinococcus radiodurans R1 section 68 of 229 of the complete chromosome 1 1869 6987 12172 0.6 4.5OE-01 AE001931.1 NT Deinococcus radiodurans R1 section 68 of 229 of the complete chromosome 1 2841 7992 13090 4.9 4.5OE-01 AA677086.1 EST-HUMAN 455d02.sl Soare5_fetal- tiver_spleen-1NFLS-S1 Homo sapiens cDNA clone IMAGE:454179 3' 3275 8420 13528 4,0 4.5OE-01 Q05793 SWISSPROT BASEMENT MEMBRANE-SPECIFIC HEPARAN SULFATE PROTEOGLYCAN CORE PROTEIN PRECURSOR (HSPG) (PERLECAN) (PLC) 3331 8475 13588 1.1 4.5OE-01 AF126378.1 NT Mus muscutus DNA polymerase epsilon catalyfic subunit (Pole) gene, exons 2 through 12 3942 9075 1,4 4.5OE-01 Q28247 SWISSPROT COLLAGEN ALPHA 5(1V) CHAIN 3982 9114 14197 1.1 4.5OE-01 A1708908.1 EST HUMAN as96c09.xl Barstead aorta HPLR86 Homo sapiens cDNA clone IMAGE:2353480 3' 4085 9212 4.0 4.5OE-01 AW873495.1 EST-HUMAN ho60g02.xl Soares NFL T G151Q_SI Homo saplens cDNA done IMAGE:3041810 3, 1991 7107 0.9 4.4OE-01 6680503 NT Mus musculus Integral membrane- assodated protein 1 (Itmapl), mRNA 2340 7442 12650 1.3 4.4OE-01 P49765 SWISSPROT VASCULAR ENDOTHELIAL GROWTH FACTOR B PRECURSOR (VEGF-13) (VEGF RELATED FACTOR) 3274 8419 13526 1.0 4.4OE-01 AF058790.1 NT Rattus norvegicus SynGAP:b mRNA, complete cds 3274 8419 5_27 1.0 4.4OE-01 AF058790.1 NT Rattus noryegicus SynGAP-b mRNA, complete ods 9267 1.3 4.4012-01 BE378707.1 EST HUMAN - 601237139F1 N1H MGC 44 Homo sapiens cDNA done IMAGE:3609393 5' Page 16 of 196 1 Table 4

* 4464 98-4 8,5 4.4OE-01 ALI 15324.1 NT Botryds cinerea strain T4 d3NA library under conditions of nitrogen deprivation 4924 10031 0.9 4.4OE-01 BE141396.1 EST HUMAN MRO-HT0078-131299-007-905 HT0078 Horno sapiens cIDNA 405 5573 10710 0.7 4.3OE-01 AF155218.1 NT Callithrix jacchus MWILW opsin gene, upstream flanking region 405 5573 10711 0.7 4.3012-01 AF155218.1 NT Callithrix jacchus MWILW opsin gene, upstream flanking region 961 6106 11264 0.7 4.3OE-01 A1281909.1 EST HUMAN qt82d03.xl NCI-CGAf_Col4 Homo sapiens cDNA clone IMAGE: 1961765 3' similar to contains Alu repetitive element; 1585 6711 11882 1.1 4.3OE-01 AW866550.1 EST HUMAN QV4-SNO024.200400-183- b01 SNO024 Homo sapiens cDNA 2843 7994 1.0 4.3OE-01 AW935269.1 EST HUMAN CM2-DT0003-010200-077-c01 DT0003 Homo sapiens cIDNA 3022 8174 13274 0.9 4.3OE-01 AW999477.1 EST HUMAN MRO-BN0070-270300-008g04 BN0070 Homo sapieris cDNA 3221 8369 1.5 4.3OE-01 AE000698.1 NT Aquifex aeolicus section 30 of 109 of the complete genome 4059 9186 14269 1.2 4.3OE-01 J00306.1 NT Human somatostafin 1 gene and flanks 4138 9264 14345 1.1 4.3OE-01 A13028026.1 NT Nicotlana tabacurn mRNA for granule-bound starch synthase, partial cds 4306 5573 10710 1.0 4.3OE-01 AF155218.1 NT Callithrix jacchus MWILW opsin gene. upstream flanking region 4306 5573 10711 1.0 4.3OE-01 AF155218.1 NT Callithrix jacchus MWILW opsin gene, upstream flanking region 4875 9982 1,1 4.3OE-01 A1.161502.2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 14 5017 10119 15191 2,1 4.3OE-01 Y1 5839.1 NT Cochilobolus heterostrophus pkcl, fatl genes 1335 7913 11631 1.0 4.2OE-01 Q39102 SWISSPROT CELL DIVISION PROTEIN FTSH HOMOLOG PRECURSOR 1904 7022 0.9 4.2OE-01 AA761653.1 EST HUMAN nz24a09.sl NCI C_GC131 Homo sapiens cDNA done IMAGE:1288696 3, 3558 8697 13795 4.8 4.2012-01 AE003947.1 NT - Xylella fastidlosa, section 93 of 229 of the complete genome 3585 8724 13820 1.1 4.2OE-01 A1280338.1 EST HUMAN q194b01.xl Soares Nhi- IMPu S1 Horno sapiens cIDNA done IMAGE:1 879945 3 3803 8940 14035 1.0 4.2OE-01 AW835527.1 EST---HUMAN OVO-LT001 5-180200127-h01 LT001 5 Homo sapiens cDNA 4545 9664 3.6 4.2OE-01 BE073574.1 EST-HUMAN RCS-BT0559-020300-013-E06 BT0559 Homo sapiens cIDNA 4599 9713 14805 5.0 4.2OE-01 AA534093.1 EST HUMAN nj69hOl.sl NCIWAP-Pr10 Homo sapiens cDNA clone IMAGE:997777 similar to gb:M33600 HLA CLASS 11 HISTOCOMPATIBILITY ANTIGEN, DR-1 BETA CHAIN (HUMAN); 4671 9783 14882 3.1 4.2OE-01 R13467.1 EST HUMAN yf77e01xl Soares infant brain 1 NIB Homo sapiens cDNA done IMAGE:28278 5' 1078 6216 11367 0,6 4.10 - 1 A1905481.1 EST - HUMAN RC-BT091 -210199-142 BT091 Homo sapiens cIDNA 1592 6718 11888 1.0 4.1OE-01 A1905949.1 EST HUMAN PM-BT103-270499-684 BT103 Homo saplens cDNA 1 2678 7765 12966 0.6 4.1OE-01 7705283 NT Homo saplens anaphase-promoting complex subunit 7 (APC7), mRNA 2910 8060 13165 1.8 4.1OE-01 AL161536.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 36 2910 8060 13166 1.8 4.1OE-01 AL161536.2 NT Arabidopsis thaliana DNA chromosome 4, dontig fragment No. 36 3263 8410 13517 1.1 4.1OE-01 AA906344.1 EST-HUMAN cj94b08.sl Soares_NFL T GBC S1 Homo sapiens cDNA clone IMAGE:1 505943 3' 4177 9303 14384 2.6 4.1OE-01 AJ249207.1 NT Rhodococcus sp. AD45 isoG, IsoH, Isol, IsW, isoA, IsoB, isoC, isol), isoE and isoF genes 4208 9333 1,1 4.1OE-01 AA909257.1 EST-HUMAN orn33d02.sl Soares_NFL T GBC. S1 Homo sapiens cDNA done IMAGE:1542819 3' 4373 9495 0.8 4.1OE-01 R41726.1 EST HUMAN yg11b01sl Soares Infant brain 1NIB HomosaplenscDNAdone IMAGE:318143' 5185 10277 0.9 4.1OE-01 Z99124.1 NT Bacillus subtilis complete genome (section 21 of 2l): from 3999281 to 4214814 1023 6162 11315 1.3 4.OOE-01 8404656 NT Lagueus rubellus mitochondrion, complete genome 1316 6445 11611 1.0 4.OOE-01 AF203478.1 NT Drosophila melanogaster Dalmatian (dmt) mIRNA, complete cds 1 - 6596 1.5 4.0012-01 6679258 NT Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), mRNA Page 17 of 196 Table 4

1960 7931 12274 1.2 4.OOE-01 Z96933.1 NT Ascobolus immersus masc2 gene 1960 7931 12275 1.2 4.OOE-01 Z96933.1 NT Ascobolus immersus masc2 gene 2774 5332 10463 1.1 4.OOE-01 6678490 NT Mus musculus ubiquidn-protein ligase e3 componen n-recognin (Ubrl), mRNA 2939 8090 13197 2.2 4.00IE-01 AL1 63280.2 NT Homo sapiens chromosome 21 segment HS21 C080 2939 8090 13198 2,2 4.OOE-01 AL1 63280,2 NT Homo sapiens chromosome 21 segment HS21 C080 3637 8776 13871 1.6 4.OOE-01 AF068903.1 NT Streptococcus pneumonlae Y1IC (y1IC), Y1113 (y1ID), penicillin-binding protein 2x (pbp2x), and undecaprenyl-phosphate-UDP-MurNAc,pentapepfide phospho-MurNAcpentapeptide transferase (mraY) genes, complete cds 3755 8893 13983 3.2 4.0012-01 AJ27751 1.1 NT Ovis aries partial M2 gene for T cell receptor delta chain (TCRDJ2), exon 1 3755 8893 13984 3.2 4.00IE-01 AJ27751 1.1 NT Ovis aries partial M2 gene for T cell receptor delta chain (TCRDJ2), exon 1 4712 9824 7.3 4.OOE-01 Q31849 SWISSPROT NADH-PLASTOQUINONE OXIDOREDUCTASE CHAIN 5, CHLOROPLAST 227 5418 10539 0.5 3.9OE-01 AW352188.1 EST HUMAN CM4-HT0136-150999-014- f09 HT0136 Homo sapiens cD"A 1356 6484 11651 0.8 3.9OE-01 AF206618.1 NT Gorilla gorilla carboo-ester lipase (CEL) gene, complete cds 2605 7695 12899 1.3 3.9OE-01 A13033019.1 NT Homo sapiens mRNA for K1AA1 193 protein, partial cds 2673 7760 12959 2.2 3.9OE-01 X82032.1 NT H.sapiens B-myb gene 2673 7760 12960 2.2 3.9OE-01 X82032.1 NT H.sapiens B-rnyb gene 3058 8209 13308 3.6 3.9OE-01 AJ225896.1 NT Sinorhizoblurn melilofi egi, syr132, cya3 genes and orf3 154 5350 2.5 3.8OE-01 7019488 NT Homo sapiens protein kinase PKNbeta (pknbeta), mRNA 805 5956 11109 0.7 3.8OE-01 AJ277435.1 NT Olive latent ringspot virus genomic RNA for polyprotein gene 848 5997 0.7 3.8OE-01 AJ277435.1 NT Olive latent dngspot virus genomic RNA for polyproteln gene 1827 6946 1.2 3.8OE-01 AE003870.1 NT Xylelia fastidiosa, section 16 of 229 of the complete genome 2521 7617 12818 1.2 3.8OE-01 AB026898.1 NT Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 112 (DLEC1, ORCTIL3, ORCTIL4 genes, complete cds) 2524 7620 12821 0.9 3.8OE-01 AF214117.1 NT Arabidopsis thaliana putative c-myb-like transcription factor (MY133R-3) mRNA, complete cds 2591 7948 12884 1.6 3.8OE-01 6678002 NT Mus musculus solute carrier family 1, member 6 (Slcla6), mRNA 2972 8123 0.9 3.8OE-01 AJ251057.1 NT Human immunodeficiency virus type 1 complete genome (isolate 98SE-MP1213) 3010 8162 13264 2.0 3.8OE-01 AF043383.1 NT Pleuronectes americanus aminopeptidase N (ampN) gene, partial cds 3436 0580 13692 7.8 3.8OE-01 AL161518.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 30 3494 8635 0.8 3.8OE-01 A1807219.1 EST HUMAN wf38bl 2.xl Soares_WIL T GBC S1 Horno sapiens cDNA clone IMAGE:2357855 3' 3506 6635 0.9 3.8OE-01 A1807219.1 EST HUMAN wf38bl 2.xl Soares_INIFL T GBC S1 Homo sapiens cDNA clone IMAGE:2357855 3' 3841 8977 14077 0.9 3.8OE-01 6754095 NT Mus musculus general transcription factor 111 (Gtf21), mRNA 2131 7241 12449 1.2 3.7OE-01 AF162061.1 NT Capnlomyces stellatus beta- tubulin (btub) gene, partial cds 2431 7530 12735 3.6 3.7OE-01 A13037831.1 NT Homo sapiens mIRNA for KIAA1410 protein, partial cds 3414 8556 13665 9.5 3.7OE-01 AF056336.1 NT Danio redo bone morphogenefic protein 4 precursor (BIVIP4) gene, complete cds 4136 9262 14344 6.6 3.7OE-01 A1218707.1 EST-HUMAN ok39c07.xl Sdares- NSFFEL9W_07_PA-P-81 Homo sapiens cDNA clone IMAGE:1510188 3' 4225 9349 14430 1.5 3.7OE-01 AW878037.1 EST HWAN MR3-OT0007-080300-104- b02 OT0007 Horno sapiens cDNA 4292 9416 14506 3.3 3.7OE-01 AE002408.1 NT Neisseria meningiddis serogroup B strain MC58 section 50 of 206 of the complete genome 124 2.4 3.6OE-01 U89241.1 NT Human mlbp gene, partial cds --- Page 18 of 196 Tabie 4 1 ks- C 1 1292 6421 11586 0.9 3.6OE-01 T80255.11 EST HUMAN yd03e05xl Soares infant brain 1 NIB Homo sapiens cDNA done IMAGE:24443 S' 1292 6421 11587 0.9 3.6OE-01 T80255.1 EST HUMAN y003e05.rl Soares infant brain 1 NIB Homo sapiens cDNA done IMAGE:24443 5' 1874 6992 12177 2.2 3.6OE-01 AW590184.1 EST-HUMAN hg33f02.xl NCI CGAP GC6 Homo sapiens cDNA clone IMAGE:2947419 3' 1874 6992 12178 2.2 3.6OE-01 AW590184.1 EST-HUMAN hg33f02.xl NCI CGAP GC6 Homo saplens cDNA done IMAGE:2947419 3' 1908 7025 12214 2.2 3.6OE-01 AF216207.1 NT Mus musculus ribosomal protein S1 g (Rps19) gene, complete cds 2007 7121 1.1 3.6015-01 AF056927.1 NT Rattus norvegious repeat element associated vAth the Rasgrfl gene 2223 7330 1.0 3.6OE-01 A13002321.1 NT Human mRNA for KIAA0323 gene, partial cds 2341 7443 0.9 3.6OE-01 X76725,11 NT P.Irregulare (P3804) gene for actin 2437 7536 12743 0.6 3.6OE-01 AW812033.1 EST-HUMAN RC.5-ST0171-181099-011- 907 ST0171 Homo sapiens cDNA 2589 7682 12882 0.8 3.6OE-01 P24206 SWISSPROT PROTEIN-L-ISOASPARTATE 0METHYLTRANSFERASE (PROTEIN-BETA-ASPARTATE METHYLTRANSFERASE) (PIMT) (PROTEIN L-ISOASPARTYL METHYLTRANSFERASE) (L-ISOASPARTYL PROTEIN CARBOXYL METHY1-TRANSFERASE) 2869 8020 8.9 3.6OE-01 AF199485.1 NT Drosophila melanogaster sugar transporter 3 (sut3) mRNA, complete cds 3424 8566 13677 -1,9 3.6OE-01 X76758,11 NT H.sapiens serotonin transporter gene, exons 9 and 10 3424 8566 13678 1.9 3.6OE-01 X76758.1 NT H.sapiens serolonin transporter gene, exons 9 and 10 4192 9318 14402 0.9 3.6OE-01 BE251129.1 EST-HUMAN 601107183F1 NIH MGC 16 Homo saplens cIDNA done IMAGE:3343484 5' 4192 93118 14403 0.9 3.6OE-01 BE251129.1 EST HUMAN 601107183F1 NIH MGC_116 Homo sapiens cDNA done IMAGE:3343484 6 4635 9749 14844 0.9 3.6OE-01 AJ009609.1 NT Brassica napus mRNA for MAP4K alpha2 protein 4684 9796 14899 1.2 3.6OE-01 AJ229237.1 NT Bacteria from anoxic bulk soil 16S rRNA gene (strain XB45) 4941 10047 15119 2.0 3.6OE-01 AW339393.1 EST HUMAN ha02g04.xl NCI CGAP G24 Horno sapiens cDNA done IMAGE.2872566 3, 202 5396 10522 0.7 3.5OE-01 6678933 NT Mus musculus mannose receptor, C type 2 (Mrc2), mRNA 662 5818 10946 1.0 3.5OE-01 AL161581.2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 77 708 5862 10998 1.2 3.5OE-01 7706136 NT Homo sapiens GAP-like protein (LOC51306), mRNA 708 5862 10999 1.2 3.5OE-01 7706136 NT Homo sapiens GAP-like protein (LOC51306), mRNA 1622 6748 11916 1.1 3.5OE-01 U35776.1 NT Rattus norvegicus ADP- ribosylation factor-directed GTPase activating protein mRNA, complete cds 2563 7947 12859 0,7 3.5OE-01 AA223252.1 EST HUMAN zrO8a09.sl Stratagene NT2 neuronal precursor 937230 Homo saplens cDNA done IMAGE:650872 3' 2672 7759 0.9 3.5OE-01 U05897.1 NT Fibrobacter succinogenes S85 endoglucanase E (celE) and endoglucanase D (ceID) gene, complete cds 4165 9291 14371 1.9 3.5OE-01 AF071253.1 NT Danio redo homeobox protein (hoxb5b)_gene, complete cds 4379 9501 14588 1.3 3.5OE-01 BE146585.1 EST HUMAN RCS-HT0218-181090,01 1 - g02 HT0218 Homo sapiens cDNA 4790 9901 14994 0.8 3.5OE-01 N81203.1 EST-HUMAN 788iEl fetal brain cDNA Homo sapiens cIDNA done 788iEl-K similar to R07879, Z40498 4849 9956 15045 3.8 3.5012-01 M118349.1 NT Rat leukocyte common antigen (L-CA) gene, exons 1 through 5 691 5846 0.7 3.4OE-01 AJ242956.1 NT Homo sapiens partial N-myc (exon 3), HPV45 L2, HPV45 Ll, HPV45 E6, HPV45 E7 and HPV45 E1 genes isolated from 1C4 cervical carcinoma cell line 960 6105 11263 1.5 3.4OE-01 Y09798.2 NT Pseudomonas fluorescens coiR, colS genes, orf222 and partial inaA gene 1304 6433 11598 0.6 3.4OE-01 Y00554.1 NT Azotobacter vinelandri nifA gene for NifA protein (positive regulatory element) Page 19 of 196 Table 4

1321 6450 11615 0.6 3.4OE-01 AF057063.1 NT ErvAnia carotovora subsp. carotovora aspartate aminotransferase (aat) gene, partial cds; HexA (hexA), NADH dehydrogenase chain A (nuoA), and NADH dehydrogenase chain'B (nuoB) genes, complete cds; and NADH dehydrogenase chain C (nuoC) gene, partial cds 2352 7454 12661 1.0 3.4OE-01 D90909.1 NT Synechocystis sp. PCC6803 complete genome, 11127,1311235-1430418 2642 7731 12932 1.5 3.4OE-01 AL161516.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 28 2679 7766 12967 1.1 3.4OE-01 AJ251835.1 NT Mus musculus Kcnql, Ltirpc5, Mash2, Tapa-1, Tssc4 and Tssc6 genes, aftemadve transcripts 3124 8273 13378 5.4 3.4OE-01 U83905.1 NT Canis familiads rod photoreceptor cGIVIP-gated channel alpha-subunit (CNGCI) mRNA, complete cds 3488 8629 13741 3.5 3.4OE-01 AF106835.1 NT Methylovorus; sp. strain SS1 putative GrpE (grpE), DnaK (dnaK), and putative DnaJ (dnaJ) genes, complete cds 3965 9097 1.2 3.4OE-01 AA584196.1 EST HUMAN nol 1b1O.sl NCI---PGAP Phel Homo sapiens cDNA done IMAGE:1 100347 3 4394 9516 14606 0.8 3.4OE-01 AF166341.1 NT Homo saplens Integrin alpha 6 (ITGA6) gene. exons 12 through 23 4544 9663 14753 1.8 3.4OE-01 BE069912.1 EST-HUMAN MR4-BT0403-230200-202- c01 BT0403 Homo sapiens cDNA 4645 7766 12967 2.2 3.4OE-01 AJ251835.1 NT Mus musculus Kwql, Ltrpc5, Mash2, Tapa-1, Tssc4 and Tssc6 genes, altemative transcdpts 4828 9936 15026 0.9 3.4OE-01 BE463761.1 EST-HUMAN hyl7d09.xl NC1-WAP-GC6 Homo sapiens cDNA cJone IMAGE:3197585 3'similar to contains Ll.t3 L1 repetitive element; 4870 9977 3,6 3.4OE-01 A1240973.1 EST HUMAN cd95cO5.xl NCI - CGAP-KW Homosaplens cDNA clone IMAGE: 1867208 3'slmilar to contains Alu repetitive element; 4970 10073 15146 1.2 3.4OE-01 X16544.1 NT Sea urchin hsp70 gene 11 for heat shock protein 70 14 5222 10323 1.3 3.3OE-01 X07990,1 NT Rhizobium leguminosarum sym plasmid pRL5J1 nodX gene 5222 10323 2.7 3.3OE-01 X07990.1 NT Rhizobium leguminosarum sym plasmid pRL5J1 nodX gene 440 5606 10740 1,2 3.3OE-01 AL161545.2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 45 617 5776 10898 1.4 3.3OE-01 7662485 NT Homc sapiens K1AA1 100 protein (K1AA1 100), mRNA 1182 6315 11472 1.0 3.3OE-01 Q12446 SWISSPROT PROLINE-RICH PROTEIN LAS17 1588 6714 11884 1.1 3.30E-01 6753685 NT Mus musculus disintegdn 5 (Dtgn5), mRNA 1704 6827 1.1 3.3OE-01 AA332734.1 EST HUMAN EST36722 Emb!yo, 8 week 1 Homo sapiens cDNA Vend 2355 7457 1.7 3.3OE-01 4507834 NT Homo sapiens uddine monophosphate synthetase (orotate phosphodbosyl transferase and orotdine-5!-decarboxylase) (UMPS) mRNA 2752 7836 13037 1.1 3.3OE-01 AJ132478.1 NT Chlamydomonas reinhardtil STFI gene, partial 2760 7844 13046 3.1 3.30E-01 8923039 NT Homo saplens hypothetical protein FI-J2 036 (FI-J20036), mRNA 2919 8070 13180 2.0 3.3OE-01 AJ251805.1 NT' Bactedophage phi-YeO3-12 complete genome 2982 8134 1.1 3.3OE-01 002743 SWISSPROT INTERLEUKIN-12 ALPHA CHAIN. PRECURSOR (IL-12A) (CYTOTOXIC LYMPHOCYTE MATURATION FACTOR 35 KO SUBUNIT) (CLIVIF P35) 3016 8168 13269 1.0 3.3OE-01 AJ007932.2 NT Streptomyces argillaceus mithraTydn blosynthetic genes 3450 8592 13705 1.1 3.3OE-01 AB012922.1 NT Homo sap(ens MTAl-Ll gene, complete cds 3738 8871 13965 2.2 3.3OE-01 084645 SWISSPROT EXODEOXYRIBONUCLEASE V BETA CHAIN 84 9020 14114 1.6 3.3OE-01 AL161498.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 10 38 1414 1.9 3.3OE-01 AF200446.1 NT Hypoxylon fragiforme chifin synthase gene, partial cds 3. 9054 Page 20 of 196 Table 4

4271 9396 2.7 3.3OE-01 D31662.1 NT Rattus norvegicus DNA for requcalcin, partial cds 4589 9703 1.5 3.3OE-01 A1539114.1 EST HUMAN tp78b12.xl NCI CGAP_Ut3 Homo sapiens cDNA clone IMAGE:2205407 Ysimilar to gb:X57522 ANTIGEN PEPTIDE TRANSPORTER 1 (HUMAN); 5133 10228 15303 1.0 3.3OE-01 W30992.1 EST HUMAN zb85b03xl SoareMenescent- fibroblasts-NbHSF Homo sapiens cDNA clone IMAGE:310349 5' 449 5615 0.7 3.2OE-01 AF018261.1 NT Rattus norvegicus EH domain binding protein Epsin mRNA, complete cds 701 5856 0,7 3.2OE-01 AL161561.2 NT Arabidopsis thallana DNA chromosome 4, condg fragment No. 61 1146 6281 11434 6.5 3.2OE-01 AF047013.1 NT Fusarium poae virus 1 RNA2 putative RNA dependent RNA polymerase gene, complete cds 1261 6390 11551 1.0 3.2OE-01 Z50202.1 NT P.vulgaris a -1 gene 1368 6496 11662 2.3 3.2OE-01 Q48624 SWISSPROT LACTOSE PERMEASE (LACTOSE- PROTON SYMPORT) (LACTOSE TRANSPORT PROTEIN) 1737 6860 12038 1,2 3.2OE-01 Z36041.1 NT S.cerevislae, chromosome 11 reading frame ORF YBR172c 1744 6867 12045 2.0 3.2OE-01 AW957194.1 EST HUMAN EST369264 MAGE resequences, MAG13 Homo saplens cDNA 1744 6867 12046 2.0 3.2OE-01 AW957194.1 EST HUMAN EST369264 MAGE resequences, MAG1) Homo sapiens eDNA 1800 6920 12106 1.2 3.2OE-01 AL1 11655.1 NT Botrytis dnerea strain T4 cDNA library under conditions of ni en deprivation 2494 7590 1.1 3.2OE-01 7710079 NT Mus musculus Pbynotted 1. homcobox (Pknoxl), mRNA 2677 7764 12965 0.6 3.2OE-01 AF060568.1 NT Homo sapiens promyelocytic leukernia zJnc finger protein (PLZF) gene, complete ods 3556 8695 1.0 3.2OE-01 D10872.1 NT Humarn h NAT allele 3-2 gene for arylamine N-acetyjtransferase 3868 9004 1.0 3.2012-01 AL161546.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 46 4295 9419 14509 1.4 3.2OE-01 M18818.1 NT Rabbit beta-like globin gene duster encoding the epsilon, gamma, delta (pseudogene) and beta globin polypepfides, complete cds 4375 9497 14584 0.8 3.2OE-01 AF1 11167.2 NT Horno sapiens jun dimerization protein gene, partial cds; clos; gene, complete cds; and unknown gene 4397 9519 14609 1.3 3.20E-01 Q10268 SWISSPROT HYPOTHETICAL 81.7 KID PROTEIN Cl 3G7.04C IN CHROMOSOME 1 PRECURSOR 4907 10014 1.2 3.2012-01 M32352.1 NT Mouse reni (Ren-l-d) gene, complete cds 2636, 7725 12929 1.4 3.1OE-01 R18051.1 EST HUMAN ye90h06xl Soares fetal liver spleen 1NIFI-S Homo sapiens cDNA done IMAGE: 1250515' similar to gb:M64241 CIM PROTEIN (HUMAN); 2665 7877 12951 1.3 3,1OE-01 7661971 NT Homo sapiens KIAA0174 gene Eroduct (KI 174),. mRNt 2665 7877 12952 1.3 3.1012-01 7661971 NT Homo sapiens KIAA01 74 gene product (K1AA01 74), mRNA 2827 7978 1.0 3.1OE-01 AW629036.1 EST HUMAN W46h08xl Soares_NFL T GBC S1 Homo sapiens cDNA clone IMAGE:2975391 3' 3136 8285 2.8 3.1OE-01 AB029069.1 NT Mus musculus gene for SerfThr kinase KKIAMRE, exon 6 3832 8969 14068 1.2 3.1OE-01 AJ251586.1 NT Daucus carota mRNA for transcription factor E2F (E2F gene) 5073 10171 15249 1.0 3.1OE-01 AA576308.1 EST-HUMAN nm61 h05.s11 NC1-WAP_W Homosaplens cDNA done IMAGE:1 0727613 66 7857 10398 0.5 3.OOE-01 6755083 NT Mus musculus protein kinase Q epsilon (Pkce, mRNA 254 5442 10569 3.8 3.0OE-01 AJ271735.1 NT Homo sapient Xq pseudoautosomal region; segment 112 1204 6336 11492 0.6 3.OOE-01 AW300400.1 EST HUMAN xs63f08.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:2774343 3' 1491 6618 11788 2.3 3.OOE-01 AJ006755.1 NT Balaenoptera physalus gene encoding 1 natriuretic pepfide 1179 3.OOE-01 A1803369.1 ET HUMAN tc42c05.xl Soares-totai-fetus_Nb2HF8 9w Homo sapiens cDNA clone IMAGE: 2067272 3 Page 21 of 196 Table 4

2089 7200 12405 0.9 3.OOE-01 AF237778.1 NT Rattus norvegicus Ca2+ Icalmoduiin-dependent protein kinase 11, alpha subunit mRNA, 3' untranslated region 3175 8324 1.0 3.0OE-01 AB030481.1 NT Corynebacterium sp. ALY-1 alyPG gene for polyguluronate Iyase, complete cds 3709 884713937 1.1 3.OOE-01 AF229122.1 NT Homo saplens acetylchoiinesterase collagen-like tail subunit (COLCI) gene, exons 11 A and 12, and complete cds, alternatively spliced 3789 8926 14020 1.3 3.OOE-01 AW817785.1 EST-HUMAN PM1-ST0262-261 1 99-001- g01 ST0262 Homo sapiens cDNA 4104 9230 1.7 3.OOE-01 L42123.1 NT Mus musculus fibroblast growth factor receptor 3 (FGFR3) gene, intron 9 4412 9532 14621 1.9 3.OOE-01 AJ006755.1 NT Balaenoptera physalus gene encoding atrial natriurefic pepfide 1038 6178 11331 0,5 2.9OE-01 AA090216.1 EST HUMAN chp0182.seq.F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA 5' 1100 6238 11390 0.9 2.9OE-01 BE066156.1 EST-HUMAN CMO-BT0320-261199-103- a06 BT0320 Homo sapiens cDNA 1978 7093 12294 0.6 2.9OE-01 AE000736.1 NT Aquifex aeolicus section 68 of 109 of the complete genome 2201 7310 12521 1.0 2.9OE-01 AF222718.1 NT Chrysodidymus synuroideus mitochondrion, complete genome 3147 8296 13405 1.0 2.9OE-01 AF0781 11.1 NT Xenopus laevis transcription factor E2F mRNA, complete cds 3213 8361 13471 2.3 2.9OE-01 AW754239.1 EST HUMAN PM1-CT0326-171299- 001412 CT0326 Homo sapiens cDt4A 3213 8361 13472 2.3 2.9OE-01 AW754239.1 EST-HUMAN PM1:370326-171299- 001412 CT0326 Homo sapiens cDNA 3821 8958 14054 1.3 2.9OE-01 A1610836.1 EST HUMAN tp21a11.xl NCI CGAP - Gas4 Homo sapiens cDNA clone IMAGE:2188412 Ysimilar to gb:Dl 5050 NIL--A Z6C FINGER PROTEIN (HUMAN);contains element L1 repetitive element; 3994 9126 0.8 2.9OE-01 AW002902.1 EST-HUMAN wr02f10.xl NCI CGAP GC6 Homo sapiens cDNA done IMAGE:2480395 3' 4384 9506 14591 1.0 2.9012-01 AA284468.1 EST-HUMAN zs57d12.rl NCLWAP-GW1 Homo sapiens cDNA done IMAGE:701591 5'similar to contains Alu repetitive element; 4388 9510 14596 0.8 2.9OE-01 AF1 34119.1 NT Mus musculus SKW (SW1) gene, complete cds 4388 9510 14597 0.8 2.9OE-01 AF134119.1 NT Mus musculus SKW (SW1) gene, complete cds 4729 9841 14933 0.9 2.9DE-01 AB019029.1 NT Mus musculus gene, 6omplete cds, similar to EXLM1 4903 10010 0.9 2.9012-01 AF1 19676.1 NT Mus musculus small GTP-binding protein RAB25 (Rab25) gene, complete cds 556 5719 0.6 2.8OE-01 U67136.1 NT Rattus norvegicus A-kinase anchoring protein AKAP150 mRNA, complete cds 1067 6205 11360 0.8 2.8OE-01 AF168050.1 NT Guira 9 ira oocyte maturation factor Mos (c-mos) gene, partial cds 1197 6329 11484 1.1 2.8OE-01 AF134404.1 NT Homo sapiens delta-6 fatty acid desaturase (CYIE15RP) mRNA, complete cds 1256 6385 11545 1.1 2.8OE-01 BE313442.1 ESTHUMAN 601148733F1 N11- 1_1VIGQ_19 Homo sapiens cDNA clone IMAGE:3163688 5' 1256 6385 11546 1.1 2.8012-01 BE313442.1 EST-HUMAN 601148733F1 NIH MGC 19 Homo sapiens cDNA clone IMAGE:3163688 5' 1269 6398 11557 1.0 2.8OE-01 D86550.1 NT Human mRNA for sednelthreonine protein kinase, complete cds 1696 6820 12002 0.6 2.8OE-01 AW860020.1 EST-HUMAN QV1-CT0364-120200-065- b05 CT0364 Homo sapiens cDNA 1966 7082 12282 0.6 2.8OE-01 AL-047620.1 EST-HUMAN DKFZp58612321j1 586 (synonym: hutel) Homo sapiens cDNA clone DKFZp58612321 20P4 7796 12401 0.5 2.8OE-01 AW511195.1 EST HUMAN hd44b03.xl Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE:2912333 3' 2134 1 12451 0.8 2.8OE-01 AF100152.1 NT Drosophila melanogaster connector enhancer of KSR protein CNK mRNA, complete cds 7244 2243 7349 0.9 2.8OE-01 AW1 77111.1 EST-HUMAN CM1-CTO127-160899-005-d01 CTO127 Homo sapiens cDNA 2423 7522 12729 1.1 2.8OE-01 AE000494.1 NT Escherichla coli K-12 MG1655 section 384 of 400 of the complete genome 7522 12730 1.1 2.8OE-01 AE000494.1 NT Escherichia col) K-12 MG1655 section 384 of 400 of the complete genome 24253 7597 0.9 2.8OE-01 A1-161565.2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 65 2500 2 _7 12920 1.4 2.8OE-01 IAB020975.1 INT 1Arabidopsis thaliana mRNA for lipoyitransferas - c, complete cds Page 22 of 196 Table 4

1 2940 8091 1.5 2.8012-01 AF179480.11 NT Toxoplasma gondii 90k13a heatshock protein (HSP90) mRNA, partial eds 2941 8092 13199 2.5 2.8OE-01 Z14037.1 NT B.taurus microsateliM IETH121) 2941 8092 13200 2.5 2.8OE-01 Z14037.1 NT B.taurus microsatellite (ETH121) 3095 8246 13349 10.7 2.8OE-01 Z73462.1 NT A.chrysaetos mitochonddat cytochrome b gene 3332 8476 13589 1.1 2.8OE-01 AP000004.1 NT Pyrococcus horikoshil OT3 genomic DNA, 777001-994000 nt. position (417) 3704 8842 0.9 2.8OE-01 AL161593.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 89 3915 9049 14136 1.8 2.8OE-01 AE001 180.1 NT Borrella burgdorfed (section 66 of 70) of the complete genome 4103 9229 2.2 2.8012-01 A1090868.1 EST-HUMAN ov44910.xl Soares testis - NHT Homo sapiens cIDNA done IMAGE:1 640226 Ysimilar to contains Mu repetitive elementcontains element MER22 repetitive element; 4352 9474 14566 1.0 2.8OE-01 AL021127.2 NT Mus musculus chromosome X configA; putative Mageag gene, Caltractin, NAD(P) steroid dehydrogenase and Zinc finger protein 185 4357 9479 14570 2.2 2.8OE-01 P13615 SWISSPROT RNA POLYMERASE BETA SUBUNIT (LARGE STRUCTURAL PROTEIN) (L PROTEIN) 4421 9541 14633 1.7 2.8OE-01 U58881.1 NT Mus musculus p53 binding protein 2 homolog (p5313132), partial cDNA 4525 9644 14734 0.8 2.8OE-01 AF032870.1 NT Drosophila melanogaster Shar pei/DRhoGEF2 mRNA, complete cds 4673 9785 14884 1.0 2.8OE-01 D15050.1 NT Human mRNA for transcription factor AREB6, complete ods 4673 9785 14885 1.0 2.8OE-01 D15050.1 NT Human mRNA for transcription factor AREB6, complete cds 4722 9834 14926 2.8 2.8012-01 AF030154.1 NT Bovine adenovirus 3 complete genome 4771 10303 3,2 2.8OE-01 Y10606.1 NT M.musculus I9G2c gene for Immunoglobulin constant heavy chain, allele lg 1 4787 9898 14991 1.7 2.8OE-01 A1272669.1 EST-HUMAN q159cl 1.xl Soares- NhHMPu-Sl Homo sapiens cDNA done IMAGE:1 876628 Ysimilar to 5173 10267 15337 0.9 2.8OE-01 S69963.1 NT contains Alu repetitive element; contalns element LTR5 repetitive element; mnp2=manganese peroxidase lsozyme 2 {heat-shock elements, metal response elements) [Phanerochaete chysosporium, Genomic, 3297 nt] 5173 10267 15338 0.9 2.8OE-01 S69963.11 NT mnp2=manganese peroAdase isozyme 2 (heat-shock elements, metal response elements) [Phanerochaete chrysospodum, Genomic, 3297 nt) 468 5633 10762 0,8 2.7OE-01 Y17324.1 NT Rattus norvegicus CDK104 mRNA 597:5755 10873 1.1 2.7OE-01 AA450061.1 EST HUMAN zx39b1O.sl Soares_totaLfetus_Nb2HF_gw Homo sapiens ciDNA done IMAGE:788827 3' similar to contains Alu repetitive element; 1240 6369 11528 0.5 2.70E-01. ABOO4906.1 NT lpomoea purpurea transposable element T1p1100 gene for transposase, complete ods 1604 6731 0.6 2.7OE-01 X79815.1 NT G.1amblia SR2 gene 1698 6822 12003 1.1 2.7OE-01 W58067.1 EST HUMAN zd22h10xl Soaret_fetal- heart-NbHH19W Homo sapiens cDNA done IMAGE:341443 5' 1738 6861 12039 1,3 2.7OE-01 P03341 SWISSPROT GAG POLYPROTEIN (CONTAINS: INNER COAT PROTEIN P12; CORE PROTEIN P1 5; CORE SHELL PROTEIN P30; NUCLEOPROTEIN P10] 2088 7934 0.9 2.7OE-01 AF047575.1 NT Rattus norvegicus vesicular monoamine transporter type 2, promoter region and exon 1 2316 7419 12626 3.2 2.7012-01 Y13868.1 NT Feline Immunodefidency virus env gene. isolate ITT0088PlU (M88), partial 2406 7505 12712 1.3 2.7OE-01 A1310858.1 EST-HUMAN ta43c111.x2 NCI_PGAP- LU25 Homo sapiens cDNA done IMAGE:2046836 Ysimilar to contains element 1.1 repetitive element; 2629 7719 12919 1.5 2.7012-01 P22105 SWISSPROT TENASCIN-X PRECURSOR (TWX) (HEXABRACHION-LIKE) 39 9063 14150 1.9 2.7OE-Oi Ai928015.1 EST HUMAN wo92el 1.xl NCLWAP Kidl 1 Homo sapiens cIDNA done IMAGE:2462828 3' 9076 14158 2.3 L77569.1 NT Homo saplens DIGeorge syndrome critical region, telomeric end Page 23 of 196 Table 4

4286 9411 14498 5.1 2.7012-01 6680855 NT Mus musculus corficosteroid binding globulin.(Cbg), mRNA 4796 9907 15000 0.9 2.7OE-01 L27516.1 NT Trificum aesfivum (Wcs66) gene, complete cds 4973 10076 3.5 2.7OE-01 AW856131.1 EST-HUMAN RC1-CTO286-230200-016-e03 CTO286 Homo sapiens cDNA 5190 10281 15349 0.8 2.7OE-01 BE182123.1 EST - HUMAN PM3-HT0642-290300001-b05 HT0642 Homo sapiens cDNA 462 7889 10756 0.5 2.6OE-01 P78411 SWISSPROT IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-2 472 5638 1.4 2.6OE-01 D16459.1 NT Bos taurus mRNA for mb-1, complete cds 1355 6483 11649 1.0 2.6OE-01 4557640 NT Homo sap ns histone deacetylase 2 (HDAC2), mRNA 1355 6483 11650 1.0 2.6OE-01 4557640 NT Homo sapiens histone deacetylase 2 (HDAC2), mRNA 1418 6546 11720 1.0 2.6OE-01 A13013290.1 NT Glydne max pseudogene for Bd 30K 1854 6972 12156 3.1 2.6OE-01 AL161472.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 2 1854 6972 12157 3.1 2.6OE-01 AL161472.2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 2 2046 7159.6 2.6OE-01 AW733152.1 EST-HUMAN bb0400.xl N1H - MGC - 14 Horno, saplens cDNA done IMAGE:2958451 Ysimilar to gb:M36072 60S RIBOSOMAL PROTEIN L7A (HUMAN); gb:M114689-cds11 Mouse surfeit locus surfeit 3 protein gene (MOUSE); 2106 7217 12427 1.2 2.6OE-01 M1 1844.1 NT Human prealbumin gene, complete cds 71596 2424 7523 1.0 2.6OE-01 Y12996.1 NT B.mariflmus rbeL gene 2499 3.5 2.6OE-01 BE272440.1 EST HUMAN 601126016F1 N1H MGC 9 Homo sapiens cDNA clone IMAGE:2990043 5, 3053 8204 1.0 2.6OE-01 AW974531.1 EST HUMAN EST386635 MAGE resequences, MAGM Homo sapiens cDNA 3485 8626 0,9 2.6OE-01 BE217816.1 EST HUMAN hv30c02.xl NCI CGAP_Lu24 Horno sapiens cDNA clone IMAGE:3174914 Ysimilar to contains Ll.t3 L1 repetitive clement; 3532 8673 13777 1.1 2.6OE-01 M22342.1 NT Bacterlphage T2 DNA-(adenine- N6)methyltransferase (dam) gene, complete cds 3591 8730 13827 1,7 2.6OE-01 AF2291118.1 NT Homo sapiens acetylcholinesterase collagen-like tail subunit (COW) gene, exons 1A, 2, 3. 4, and 5 3651 8790 13883 0.9 2.6OE-01 AB017446.1 NT Rattus norvegicus mRNA for organic anion transporter 3, complete cds 4010 9141 14223 1.0 2.6OE-01 AW959510.1 EST HUMAN EST371580 MAGE resequences, MAGF Homo sapiens cDNA 4062 9189 14274 13.1 2.6OE-01 BE080598.1 EST-HUMAN QV1-BT0630-040400-132- e03 BT0630 Homo sapiens cDNA 4259 9384 14467 1.0 2.6OE-01 AF175293.1 NT Enterococcus faeclum strain N97-330 vanD glycopepfide resistance gene cluster, complete cds; and unknown gene 4393 9515 14604 0.8 2.6OE-01 A13021180.1 NT Gallus pall s mRNA for skeletal myosin heavy chain, complete cds 4393 9515 14605 0.8 2.6OE-01 A13021180.1 NT Gallus gallus mRNA for skeletal myosin heavy chain, complete cds 4448 9568 14666 1,2 2.6OE-01 AA457617.1 EST HUMAN aa89d07xl Stratagene fetal retina 937202 Homo sapiens cDNA done I MAGE:838477 5' 4548 9667 14756 1.6 2.6OE-01 U011103.1 NT Arabidopsis thaliana PSI type Ill chlorophyll alb-binding protein (Lhca31) mRNA, complete cds 4616 9730 14824 1.3 2.6OE-01 AF142703.11 NT Ophrestia radicosa maturase- like protein (matK) gene, complete cds; chloroplast gene for chloroplast product 4672 9784 14883 2.2 2.6OE-01 AW027920.1 EST HUMAN ws62h11.xl NCI CGAP - 13m23 Homo sapiens cDNA done I MAGE:2501829 3'similar to SW:ME13 MOUiE P97368 HOMEOBOX PROTEIN MEIS3; 4896 10003 15086 3.6 2.6OE-01 H04858.1 EST-HUMAN A51e05xl Soares placenta N1J2HP Homo sapiens cDNA done IMAGE:152288 10151 0.9 2.6OE-01 P08503 SWISSPROT ACYL-COA DEHYDROGENASE, MEDIUM-CHAIN SPECIFIC, MITOCHONDRIAL PRECURSOR(MCAD) Page 24 of 196 1 Table 4

241 5431 10557 0,7 2.5OE-01 4502296 NT Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (ATP5D), nuclear gene encoding mitochondrial protein, mRNA 242 5431 10557 0.6 2.5OE-01 4502296 NT Homo sapiens ATP synthase, H+ transporting, mitochonddal F1 complex, delta subunit (ATP5D), nuclear gene encoding mitochondrial protein, mRNA 255 5443 1,1 2.5012-01 M26501.1 NT Starfish (13.ochraceus) cytoplasmic actin gene, complete cds 815 5965 11121 1,3 2.5OE-01 U09964.1 NT Mus musculus ICRISYviss glyceraldehyde 3-phosphate dehydrogenase (Gapd-S) gene, complete cds 1044 6184 1.9 2.5OE-01 AE002156.1 NT Ureaplasma urealyficum section 57 of 59 of the complete genome 1105 6243 11396 3.2 2.5OE-01 T89837.1 EST-HUMAN yel 1g07.rl Stratagene lung (#937210) Homo sapiens cIDNA clone IMAGE:1 17468 5, 1503 6629 11798 0.9 2.5OE-01 AL1 15624.1 NT Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation 1697 6821 2.0 2.5OE-01 4885406 NT Homo sapiens hyperpolarization activated cyclic nudeodd"ated potassium channel 4 (HCN4) mRNA 2357 7459 3,0 2.5OE-01 AE000675.1 NT Aquifex aeolicus section 7 of 109 of the complete genome 2446 7545 1.4 2.5OE-01 AA251987.1 EST-HUMAN z51 lallrl NCJ CGAP GCBI Homo sapiens cDNA done IMAGE:684862 5' 2597 7688 12891 1.0 2.5OE-01 X95310.1 NT B.taurus mRNA for D-aspartate oxidase 3366 8509 2.9 2.5012-01 AW973471.1 EST-HUMAN EST385464 MAGE resequentes, MAGM Homo sapiens cDNA 3490 8631 13743 0.9 2.5oE-ol AF233875.1 NT Danto redo peptide YY precursor gene, complete ods 3502 8644 13752 7.9 2.5OE-01 AL161517.2 NT Arabidopsis thaliana DNA chromosome 4, condg fragment No. 29 3774 8911 14000 1,2 2.5OE-01 A1741483.1 EST-HUMAN wgi 1c07.xl Soares-NSF- FUW_OT-PkP-SI Homo sapiens cDNA done IMAGE:2364780 3' 3774 8911 14001 1.2 2.5OE-01 A1741483.1 EST HUMAN w91 1 c07.xl Soares_NSFF8-9W-OT_PkP-Sl Horno sapiens cDNA clone IMAGE:2364780 3' 3895 9030 14123 7.4 2.5OE-01 Q04912 SWISSPROT MACROPHAGE-STfMULATING PROTEIN RECEPTOR PRECURSOR (MSP RECEPTOR) (P185-RON) (CIDWII36) (CD136 ANTIGEN) 3895 9030 14124 7.4 2.5OE-01 Q04912 SWISSPROT MACROPHAGE-STIMULATING PROTEIN RECEPTOR PRECURSOR (MSP RECEPTOR) (P185-RON) (CIDWII36) (C13136 ANTIGEN) 3977 9109 0.8.2.5OE-01 P32323 SWISSPROT A-AGGLUTININ ATTACHMENT SUBUNIT PRECURSOR 4222 9346 1.2 2.5OE-01 Q03314 SWISSPROT RHIB PROTEIN 4514 9633 14725 1.0 2.5OE-01 AF242431.1 NT Mus musculus neuronal apoptosis inhibitory protein 6 (Naip6) gene, complete cds; and Naip3 gene, exons 2-9 and 11-16 4643 9757 1.1 2.5OE-01 027225 SWISSPROT MOLT-INHIBITING HORMONE PRECURSOR (MIH) 4649 9761 14856 3.8 2.50E-01 AF007768.1 NT Chodstoneura furniferana diapause associated protein 2 (DAP2) mRNA, complete cds 4698 9810 3.2 2.5OE-01 A.1230113.1 NT Mus musculus annexin V gene, intron 4 segment containing S' LTR and gag portion of MuERV-L (murine endogenous retrovirus) element 4750 9861 14953 0.9 2.5OE-01 AB01 1070.1 NT Mus musculus gene for uncoupling protein 3, V-11anking region and partial VUTR 5169 10263 15334 0.9 2.5OE-01 AW663183.1 EST-HUMAN hh75f09.yl NCl-WAP--GU1 Homo sapiens cIDNA clone IMAGE:2968649 Csimilar to contains TAR1.1:2 TARI repetitive element; 5169 10263 15335 0.9 2.5OE-01 AW663183.1 EST-HUMAN hh75f09.yl NCI - WAP_GU1 Homo sapiens cDNA done IMAGE:2968649 Csimilar to contains TARI.t2 TARI repetitive element; 541 5704 10824 0.5 2.4OE-01 AA936316.1 EST_HUMAN on70d04.sli Soares NFL T GBC S1 Homo sapiens cIDNA clone IMAGE:1562023 3' 6412 11573 5,9 2.4OE-01 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFIL1 gene Page 25 of 196 1 Table 4

1282 6412 11574 5.9 2.4OE-01 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and UTFLI gene 1362 6490 11655 1.0 2.4012-01 Y1 7293.1 NT Homo sapiens FLI-1 gene, partial 1808 6927 8.7 2.4OE-01 AF267753.1 NT Mesembryanthemum crystallinum putative potassium channel protein Mktlp mRNA, complete cds 1858 6976 12161 0.5 2.4OE-01 AF251708.1 NT Zaocys dhumnades fructose-1,6- bisphosphatase mRNA, complete cds 2091 7202 12407 0.9 2.4OE-01 AF1 11168.2 NT Homo sapiens serine palmitoyl transferase, subunit 11 gene, complete cds; and unknown genes 2122 7232 1.2 2.4OE-01 P45384 SWISSPROT IMMUNOGLOBULIN A1 PROTtASE PRECURSOR (1GA1 PROTEASE) 2215 7323 12537 0,7 2.4OE-01 AE000680.1 NT Aquifex aeolicus section 12 of 109 of the complete genome 2491 7588 12792 0.6 2.4OE-01 Z36534.1 NT D.discoldeum (Ax3-K) ponA gene 2734 7818 13023 0.8 2.4OE-01 X71783.1 NT S.pomb swi6 gene 2156 7840 13642 2.4 2.4015-01 AF030154.1 NT Bovine adenovirus 3 comelete genome 3093 8244 2.8 2.4OE-01 U72n6.1 NT Oryza longistaminata receptor kinase- like protein, family member D, and retrofit (gag/pot) genes. complete cds 3109 8259 13363 1,9 2.4OE-01 X74209.1 NT H.saplens AGT gene, Pstl fragment of Intron 4 3871 9007 14106 2.4 2.4OE-01 AJ009961.1 NT Fugu rubripes PIRGFIR1 gene, exons 1-20 4594 9708 14800 78.8 2.4OE-01 Y1 3183.1 NT Her-pesvirus saimid C-488 DNA for ORF 12 to 25 4704 9816 14913 1.3 2.40 E-01 A1123160.1 EST HUMAN qa86c07.sl Soares- fetaLheart-NbHHI9W Homo sapiens cDNA clone IMAGE:1693644 3' 4817 9926 15018 0.9 2.4OE-01 BE160080.1 EST-HUMAN QV1-HT0412-020400-136- 1510 HT0412 Homo saplens cDNA 4981 10084 15154 50.2 2.4OE-01 D00944.1 NT Hepatitis C Virus genomic RNA for polyprotein, complete ods 5101 10 I'N 15277 1.0 2.4OE-01 A1302019.1 EST HUMAN go23f01.xl NCI CGAP Lu5 Homo sapiens cDNA done IMAGE:1909369 3' 5188 10279 15347 1.4 2.4012-01 7697600 NT Mus musculus small optic lobes hornolog (Dros;oLhila) (Solh), mRNA 383 5652 10685 0.9 2.3OE-01 S75898.1 NT aromatase [Poephila guttata=zebra finches, ovary, mRNA, 3188 ntl 621 5780 1,5 2.3OE-01 U39713.1 NT Mycoplasma genitalium section 35 of 51 of the complete genome 651 5809 10935 6.8 2.3OE-01 U67596.1 NT Methanococcus jannaschil section 138 of 150 of the complete genome 792 5943 11094 4.4 2.3OE-01 AE001708.1 NT Thermotoga maritima section 20 of 136 of the complete genome 851 6000 11160 1.6 2.3OE-01 AE001708.11 NT Thermotoga marifirna section 20 of 136 of the complete genome 918 6064 11219 1.1 2.3OE-01 BE311893.1 EST-HUMAN 601142073F1 N11- 1_1VIGQ_114 Homo saplens cDNA done IMAGE:3505818 5' 1494 6620 11790 0.5 2.3OE-01 6677980 NT Musi musculus vacuolar protein sorting 4b (yeast) (Vps4b), mRNA 1546 6673 0.9 2.3OE-01 U22837.2 NT Yersinia pestis HmsH'(hmsH), HmsF (hmsF), HmsR (hmsR), and HmsS (hmsS) genes, complete cels 1586 6712 11883 1.4 2.3OE-01 A.1245480.1 NT Brassica napus sig gene for S- focus 91VS:oprotein, cultivar T2 1614 674-0.11910 1.0 2.3OE-01 Y10887.2 NT Mus musculus cdh5 gene, exon 1, partial 1999 7114 1.3 2.3015-01 A.1235353.1 NT Homo sapiens partial intron 3 of the wild type AF-41FIEL. gene 2396 7496 12701 0.6 2.3OE-01 BE297718.1 EST HUMAN 601175562F1 N11-1 MGC- 17 Homo sapiens eDNA clone IMAGE:3531015 5' 2615 7705 12904 1.0 2.3OE-01 M1 1319.1 NT Human erythropoiedn gene, complete cds 275 7841 13043 0.5 2.3OE-01 AF004708.1 NT Medicago sativa aquaporin-like transmembrane channel protein (pAR 8-1) mRNA, complete cds 7841 13044 0.5 2.3OE-01 AF004708.1 NT Medicago sativa qquaporin-like transmembrane channel protein (pAFI 8-1) mRNA, complete cds Page 26 of 196 Table 4

1 2794 6493 11659 0.9 2.3OE-01 A13015033.1 NT Marinilabilia agarovorans gyrB gene for DNA gyrase subunit B, partial cds, strain:IFO 14957 2934 8085 13193 1.3 2.3OE-01 AA601379.1 EST HUMAN nol6d06.sl NCIQGAP Phel Homo sapiens cDNA done IMAGE:1 100843 3 similar to contains AW repetitive Jernentcontains element THR repetitive clement; 2950 8101 13207 0.9 2.3OE-01 ABOO5200.1 NT Mus musculus DNA for DNA dependent protein kinase catalytic subunit, emn 69 3045 8196 5.7 2.3OE-01 R21732.1 EST-HUMAN yh21b07.sl Soares placenta Nb2HP Homo saplens cDNA done IMAGE: 130357 3' 3324 8468 13583 1.1 2.3OE-01 H69836.1 EST-HUMAN yr97h10xl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA done IMAGE:213283 5 3766 8904 13993 1.1 2.3OE-01 S82821.1 NT GSTA5=glutathione S-transferase Yc2 subunit {Treglon, Intron 11 [rats, Morris hepatoma cell line, Genomic, 2212 nt, segment 1 of 3] 3856 8992 3.7 2.3OE-01 7662133 NT. Homo sapiens KIAA0450 gene product (K1AA0450), mRNA 4253 9378 14458 0.9 2.3OE-01 R82252.1 EST HUMAN yj17f01xl Soares placenta NWHP Homo sapiens cDNA done IMAGE:149017 5' 4300 9424 3.4 2.3OE-F L78789.1 NT Mus musculus renin (Ren-1 c) gene, promoter region 4349 -9471 14563 1.0 2.3OE-01 D90899.1 NT Synecho fis sp. PCC6803 complete genome, 1127, 1-133859 4386 9508 14593 2.1 2.3OE-01 AF092535.1 NT Homo sapi. ensffiltogen- activated protein kinase p3Bdelta (PRKM13) mRNA, complete cds 4454 9574 14672 5.1 2.3OE-01 5031984 NT Horno sapiens nuclear transport factor 2 (placental protein 15) (PP1 5) mRNA 4582 9698 14792 22.6 2.3OE-01 ABOO1995.1 NT Schizosaccharomyces pombe gene for Tell p, complete cds 4956 9894 14987 0.8 2.3OE-01 J103280.1 NT Human phenAethanolamine N- methAtransferase gene, complete cds 5020 10121 15193 3.7 2.3OE-01 AE003995.1 NT Xylella fastidiosa, section 141 of 229 of the complete genome 5146 10241 15315 27.0 2.3OE-01 AE000240.1 NT Escherichla coil K-12 MG1655 section 130 of 400 of the complete genome 84 5290 10417 1.0 2.2OE-01 A1052190.1 EST HUMAN oz14a10.xl Soaresfetiliver_spieerLlNFLS-S1 Homo saplens cDNA clone IMAGE1675290 YsimilartoTR:Q13040 013040ATP-BINDING CASSETTE PROTEIN; 1545 6672 11843 1.1 2.2012-01 AF187850.1 NT Homo sapiens PPAR delia gene, promoter region 2042 7155 12361 1.0 2.2OE-01 M34640.1 NT Fresh-water sponge Ernfl alpha collagen (COLF1) gene 2133 7243 12450 1.1 2.2OE-01 AJ388469.1 NT Cobitis taenia mitchonddal cyt b gene for cytochrome b 2853 8004 13102 4,0 2.2OE-01 BE155625.1 EST HUMAN PM2-HT0353-2812WO03- a112 HT0353 Horno sapiens cDNA 2863 8004 13103 4.0 2.2OE-01 BE155625.1 EST-HUMAN PM2-HT0353-281299-003- a12 HT0353 Horno saplens cDNA 2890 8041 1 ' 6 2.2OE-01 AF020503.1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene,exon 5 3176 8325 13437 1.9 2.2OE-01 A1824368.1 ESTHUMAN tK69c09.xl NCI CGAP Utl Horno sapiens cDNA done IMAGE:2274832 3' 3346 8490 2.7 2.2OE-01 A1-161562.2 NT Arabidopsis thaliana DNA chromosome 4, cohfig fragment No. 62 3743 8880 1.1 2.2OE-01 AF155728.1 NT Mphophorus maculatus truncated Rexl retrotransposon reverse transcriptase (RT) pseudogene 4007 9138 14220 0.8 2.2OE-01 AF21339.1 Mus musculus ATP-binding issette protein (Abcb8) mRNA, partial cds 4118 9244 1,2 2.2OE-01 AF119102.1 NT Drosophila melanogaster UNC-1 19 (unc-1 19) gene, complete cds 4125 9W251 14332 5.1 2.2OE-01 AF155142.1 NT Mus musculus mixed lineage kinase 3 (M1k3) and two pore domain K+ channel subunit (Kcnk6) genes, complete cds 4166 9292 14372 2.0 2.2OE-01 AF1 17340.1 NT Mus musculus MAP kinase kinase kinase 1 (Makkl) mRNA, complete 6ds 9292 14373 2.0 2.2OE-01 AF1 17340.1 NT Mus musculus MAP kinase kinase kinase 1 (Mekkl) mRNA, complete cds 99 14465 1.2 2.2OE-01 U01307.1 NT Human scRNA (BC200 beta) pseudogene 383 Page 27 of 196.

Table 4 tYCI e- 4258 9383 14466 1.2 2.2OE-01 U01307.1 NT Human scRNA (BC200 beta) pseudogene 4399 9520 14610 1.1 2.2OE-01 Z54148.1 NT B.abortus bp26 gene 4719 9831 1.2 2.2OE-01 D50604.1 NT Human beta-"plasmic actin (ACT13P9) pseudogene 4,72-4- 9836 14928 2.5 2.2OE-01 AA211216.1 EST-HUMAN zq87c05.M Stratagene hNT neuron (#937233) Homo sapiens cDNA done IMAGE:648968 5' 4891 9998 15081 1.2 2.2OE-01 M86524.1 NT Human dystrophin gene 4975 10078 1.2 2.2OE-01 L13299.1 NT Mus musculus Anculln gene, exon 3 532 5696 10819 0.6 2.1OE-01 AA827018.1 EST-HUMAN nr89g,Og.sl NCI-CGAPPr25 Homo sapiens cIDNA clone IMAGE:1 175200 Ysimilar to gb223090 HEAT SHOCK 27 KI) PROTEIN (HUMAN); 532 5696 10820 0.6 2.1OE-01 AA827018.1 EST&-HUMAN nr89g09.sl NCI-CGAP- Pr25 Homo sapiens cDNA clone IMAGE: 1175200 Ysimilar to gb223090 HEAT SHOCK 27 KI) PROTEIN (HUMAN); 955 6101 11259 0.6 2.1OE-01 AA569289.1 EST-HUMAN nm31el I.sl NCI_WAE L1p2 Homo saplens cDNA done IMAGE:1061804 958 6103 11261 0.9 2.1012-01 AL161504.2 NT Arabidopsis thaliana DNA chromosome 4. contiq fragment No, 16 1108 6245 0,7 2.1OE-01 AE002314.2 NT Chlamydia muddarum, section 45 of 85 of the complete genome 1180 6313 11468 1.2 2.1OE-01 6754299 NT Mus musculus Interferon (alpha and beta) receptor 2 (Ifnar2), mRNA 1180 6313 11469 1.2 2.1OE-01 6754299 NT Mus musculus Interferon (alpha and beta) receptor 2 (Ifnar2), mRNA 1467 6594 11771 1.3 2.1OE-01 AE001988.1 NT Delnococcus radjodurans R1 section 125 of 229 of the complete chromosome 1 1792 6912 12097 1.4 2.1OE-01 6981303 NT Rattus norvegicus Olfaitory marker protein (Omp), mRNA 1871 6989 12174 0.7 2.1OE-01 AA906824.1 EST-HUMAN ok73e02.sl NCLWAP-GC4 Homo sapiens cDNA done IMAGE:1519610 3'similar to gb:1(02765 COMPLEMENT C3 PRECURSOR (HUMAN); 2894 8045 13145 1.7 2.1OE-01 6912445 NT Homo sapiens potassium voltage- gated channel, subfamily H (eag-related), member 4 (KCNH4), mRNA 3218 8366 13478 2.0 2.1OE-01 AF06951 1.1 NT Rattus norvegicus putative sodium bicarbonate cotranspcTter (NBC) mRNA, complete cds 3967 9099 14183 1.0 2.1OE-01 P11675 SWISSPROT IMMEDIATE-EARLY PROTEIN 1E180 3967 9099 14184 1.0 2.1012-01 P11675 SWISSPROT IMMEDIATE-EARLY PROTEIN 1E180 4279 9404 1.3 2.1OE-01 AB033041.1 NT Homo sapiens mRNA for. K1AA1 215 protein, partial cds 4474 9594 14689 1.2 2.1OE-01 AB010273.1 NT Homo sapiens pshsp47 gene, complete cds 4766 9877 14971 1.3 2.1OE-01 AJ009794.1 NT Homo saplens hoxl 1 proto- oncogene, exons 1 to 3 and hug-1 gene 5134 10229 15304 1.0 2.1OE-01 M98261.1 NT Saccharomyces cerevislae tau 138 (M3) gene, complete cds 193 5387 10515 0.6 2.OOE-01 A13017437.1 NT Gallus gallus mRNA for avena, complete eds 365 5543 10675 0.9 2.OOE-01 A1475599.1 EST-HUMAN W92912.yl NCI - CGAP_CLL1 Homo sapiens cIDNA done IMAGE:2073670 5'similar to contains Alu repetitive element., 365 5543 10676 0,9 2.OOE-01 A1475599.1 EST-HUMAN te92g12.yl NCl-WAP-CLI-1 Homo sapiens cDNA clone IMAGE:2073670 5'simiiar to contains Alu repetitive element; 523 5687 0.8 2.0OE-01 7705601 NT Homo sapiens CGI-18 protein (LOC51008), mRNA 684 5839 10972 1.2 2.0OE-01 M77085.1 NT O.cunniculus gormline 19H heavy chain V-H pseudogene, allotype V1-1a2 793 5944 11095 0.6 2.ODE-01 AF027865.1 NT Mus musculus Major Histocompatibility Locus class 11 region 995 6137 11291 1.0 2.OOE-01 D90905.1 NT Synechocystis sp. PCC6803 complete genome, 7127, 781449-920915 1082 6220 11371 1.3 2.OOE-0 piens A kinase (PRKA) anchor protein (graviny 12 (AKAP12), mRNA U 11308 6 46 3ns chromosome 21 segment HS21C013 Page 28 of 196 Tabie 4 1234 6363 11525 1.4 2.OOE-01 AJ1132695.5 NT Homo sapiens racl gene 1286 6416 11579 1.3 2.OOE-01 AW384937.1 EST-HUMAN PM1-HT0422-29129M02-c06 HT0422 Homo sapiens cDNA 1419 6547 11721 0.8 2.0OE-011 M64239.1 NT Mouse T-cell receptor alphaldelta chain locus 1419 6547 11722 0.8 2.OOE-01 M64239.11 NT Mouse T-cell receptor alphaldelta chain locus 1471 6598 11773 4.9 2.OOE-01 4503408 NT Homo saplens dystrobrevin, alpha (DTNA), mRNA 1534 6660 11827 0.9 2.OOE-01 AB007974.1 NT Homo sapiens mRNA, chromosome 1 specific transcript KIAA0505 1538 6664 11832 0.6 2.OOE-01 AF260700.1 NT Homo sapiens sodiumfiodide symporter mRNA, partial ods 1688 6812 0.7 2.OOE-01 AF1 11170.3 NT Horno saplens 14g32 Jagged2 gene, complete cds; and unknown gene 1723 6846 0.6 2.OOE-01 U67525.1 NT Methanococcus jannaschil section 67 of 150 of the complete genome 2299 7403 0.6 2.OOE-01 X82877.1 NT H.sapiens Na±D-glucose cotransport regulatorgene 2861 8012 1.0 2.OOE-01 AF074990.1 NT Homo saplens full length Insert ciDNA YH85A1 1 3442 8584 13698 0.8 2.0OE-01 P46607 SWISSPROT HOMEOBOX PROTEIN GLABRA2 (HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-10) (HD-ZIP PROTEIN ATHB-10) 3520 8661 0.9 2.OOE-01 AW238.005.1 EST HUMAN xp115b02,xl NCI_PGAP-HN9 Homo saions cDNA clone IMAGE:2740395 Ysimilar to contains element MER21 repetitive element,' 3646 8785 13878 0.8 2.0OE-011 P34641 SWISSPROT CED-11. PROTEIN 3945 9078 14160 0.8 2.OOE-01 X83997.1 NT C.parasifica eapC gene 4367 9489 14580 0.9 2.OOE-01 AF060195.1 NT Mus musculus proteasome regulator PA28 beta subunit gene, complete cds 4859 9966 15051 1.1 2.OOE-01 AF147083.1 NT Homo sapiens gamma-glutamA hydrolase gene, exons 8 and 9 and complete cds 4972 10075 15148 6.1 2.OOE-01 8922080 NT Horno saplens hypothetical protein ASH1 (ASH1), mRNA 5041 10141 15219 1.1 2.OOE-01 Y19216.1 NT Horno, sapiens putative psihHbD pseudogene for hair keratin, exons 1 to 9 5308 3.5 1.9OE-01 7549743 NT Rattus norvegicus Aryl hydrocarbon receptor nuclear translocator 1 (Amtl), mRNA 349 5530 10658 2.0 1.90EM AF004353.1 NT Mus Musculus pale ear (ep) gene, vAld type allele, 31 region, pargal cds 470 5635 10764 0.7 1.9OE-01 043474 SWISSPROT KRUPPEL-LIKE FACTOR 4 (EPITHELIAL ZINC-FINGER PROTEIN W) 640 5799 10924 1.3 1.9OE-01 U32581.2 NT Homo saple s lambdaftota protein kinase C-Interacting protein mRNA, complete cds 640 5799 10925 1.3 1.9OE-01 U32581.2 NT Homo saplens lambda/lota protein kinase C-Interacting protein rARNA, complete cds 647.5806 10932 1.8 1.90E-01 BE070801.1 EST-HUMAN RC3-13T0502-251 199-01 1- d01 BT0502 Homo sapiens cIDNA M 5806 10932 2.5 1.9OE-01 BE070801.1 EST HUMAN RC3-BT0502-251199-01 1 - d01 BT0502 Homo sapiens cDNA 970 6115 0.6 1.90E-01 7305180 NT Mus musculus Interleukin 2 receptor, gamma chain (112rg), mRNA 1088 6226 11378 3.2 1.90E-01 AA358813.1 EST HUMAN EST67784 Fetal lung 11 Homo sapiens cDNA Vend 1348 6476 11642 0.8 1.90E-01 AF0611282.1 NT Sorghum bicolor 22 kDa kafidn duster 1416 6544 1.4 1.9OE-01 AF184623.1 NT Plasmodium vivax reficulocyte binding protein-2 (rbp-2) gerfe, complete cds 1873 6991 12176 0.6 1.9OE-01 AW821631.1 EST HUMAN IL2-ST0311-270300- 059E04 ST0311 Homo saplens cDNA 1928 7045 12236 0.9 1.9OE-01 AF261940.1 NT Spinacia oleracea Alwarodbosomal protein 5 precursor (Psrp-5) mRNA, complete cds; nuclear gene for chloroplast product 2330 7433 12641 1.2 1.9OE-01 8922533 NT Homo sapiens hypothetical protein FLJ10581 (FL.110581), mRNA 2892 8043 13143 3.9 1.9OE-01 U66066.1 NT Slamodon hispidus p53 gene, partial cds 2908 8058 5.6 1.9OE-01 J00922.1 NT Gallus gallus ovalbumin (Y) gene, complete cds 3349 8493 13604 3.4 1.9OE-01 D131197.1 NT Mouse gene for immunoglobulin diversity region D1 8576 13688 4.6 1.90E-01 R16467.1 EST HUMAN yf42f11 O.rl Scares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE: 129547 5' t Page 29 of 196 Table 4

C4 3735 8872 13964 0.9 1.9OE-01 AF264017.1 NT Rattus norvegicus arylacetamide deacetyfase gene, complete cds 3762 8900 13989 1.0 1.9OE-01 P39768 SWISSPROT PAIR-RULE PROTEIN ODD- PAIRED 3910 9045 14132 3.0 1.9OE-01 ABOO6784.1 NT Schizosaccharomyces pombe DNA for cytoplasmic dynein heavy chain, complete cds 3992 9124 14208 1.9 1.9OE-01 AW754106.1 EST-HUMAN CM3-CT0315-271199-045- bl 1 CT031 5 Homo sapiens cDNA 4369 9491 14581 0.9 1.9OE-01 AL1 61493,2 NT Arabidopsis thallana DNA chromosome 4, contig fragment No. 5 4662 9774 14870 0.8 1.90E-01 Z93780.1 NT Fugu rubripes genes encoding carbamoyl phosphate synthetase Ill, myosin light chain, MAP2 4663 9775 14871 2.4 1.9OE-01 AW821631.1 EST-HUMAN IL2-ST0311-270300-059- E04 ST0311 Homo sapiens cDNA 4857 9964 15049 2.1 1.9OE-01 U31785.1 NT Human papillomavirus type 36, complete genome 4912 10019 15098 0.9 1.9OE-01 AW849203.1 EST-HUMAN IL3-CTO215-180200-087- DO2 CT0215 Homo, sapiens cDNA 4943 10049 1.0 1.9OE-01 AF223642.1 NT Rattus norvegicus chemokine receptor CXCR3 mRNA, complete cds 4962 10065 15137 1.1 1.9OE-01 095239 SWISSPROT KINESIN-LIKE PROTEIN KIF4 5113 10209 15287 1.0 1.90E-01 Z70296.1 NT S.mansoil elastase HP1 gene 5123 10219 1.2 1.9OE-01 A1631199.1 EST---HUMAN ts93g12.xl NCJ - CGAP_GC6 Horno sapiens cDNA clone IMAGE:2238886 Ysimilar to gb:M21574 ALPHA PLATELET-DERIVED GROWTH FACTOR RECEPTOR PRECURSOR (HUMAN); 1 5153 10248 15321 1.0 1.9OE-01 6679095 NT Mus musculus Notch gene hornolog 3, (Drosophila) (Notch3), mRNA 17 5226 10327 0.7 1.8OE-01 X79037.1 NT H.sapiens Y315 gene 29 5238 10343 0.8 1.80E-01 U73200.1 NT Mus musculus pl 16Rip mRNA, complete cds 260 7885 10573 1.2 1.8OE-01 A8022090.1 NT Mus mu;culus CcIp gene for chaperonin containing TCP-1 gamma subunit, partial ods 366 5544 10677 0.8 1.8OE-01 4502532 NT Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2) MRNA, and translated products 729 5883 11025 0.7 1.8OE-01 AB021490.2 NT oryzjas latipes gene for membrane quanylyl cyclase 01GC1, complete eds 966 6111 11267 0.9 1.8OE-01 A19112212.1 EST-HUMAN wd71f02.xl NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE:2337051 3' 1075 6212 11364 1.2 1.8OE-01 AF000580.1 NT Dictyostelium discoldeum plasmid Ddp5, complete genome 1267 6396 11556 1.8 1.8OE-01 AL1 17189.1 NT Yersinia pestis plasmid pCD1 1487 6614 11784 1.3 1.8OE-01 6753947 NT Mus musculus quanylate nudeofide binding protein 1 (Gbpl), mRNA 1487 6F14 11785 1.3 1.8OE-01 6753947 NT Mus musculus quanylate nudeodde binding protein 1 (Gbpl), mRNA 1803 6922 0.9 1.8DE-01 4505036 NT Homo sapiens latent transforming growth factor beta binding protein 4 (LT13P4) mRNA 1823 6942 0.7 1.8OE-01 A1733708.1 EST-HUMAN q922d 1 U5 NCI CGAP - K10 Homo sapiens cDNA c4one IMAGE:1 761811 Ysimilar to TR:075936 07j936 GAMMA BUTYROBETAINE HYDROXYLASE, 2421 7520 1.5 1.8OE-01 J03216.1 NT Ti plasmid virB operon encoding twelve virulence genes, complete cds 2660 7749 1.1 1.8OE-01 AW935728.1 EST-HUMAN QV3-DT0018-081299-036-904 DT0018 Horno sapJens eDNA 2868 8019 1.6 1.8OE-01 AF184589.7 NT Jonopsidium acaule LEAFY protein (LEAFY2) gene, partial eds 2873 8024 13127 1.1 1.8OE-01 AW182300.1 EST-HUMAN x141a03.xl Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE 2659756 3' 3006 8158 4.9 1.8OE-01 P13983 SWISSPROT EXTENSIN PRECURSOR (CELL WALL HYDROXYPROLINE-RICH GLYCOPROTEIN) 3040 5728 10327 2.0 1.8OE-01 X79037.1 NT H.sapiens Y315 gene 3085 8236 13335 1.3 1.8OE-01 AW995178.1 EST-HUMAN QVMN0041-070300-147-c04 BN0041 Horno saplens cDNA 3570 8709 13807 1.1 1.6GE-01 H03369.11 EST -HUMAN yj45eOl.sl Soares placenta Nb211P Homo sapiens cDNA done IMAGE:151704 3' similarr too contains Alu repetitive element; Page 30 of 196 Table 4

3570 8709 13808 1.1 1,8OE-01 H03369.1 EST-HUMAN A45e01M Soares placenta Nb2HPHomOsapienscDNA done IMAGE:1517043'similar to contains Alu repetitive element:

4154 9280 14360 0.8 1.8OE-01 AJ271735.1 NT Homo sapiens Xq pseudoautosomal. region; segment 112 4238 9363 1.1 1.8OE-01 D37954.1 NT Bovine NB25 mRNA for MHC class 11 (SoLA-DOS), complete ods 4456 9576 5.1 1.8OE-01 AL161556.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 56 4595 9709 14801 1.1 1.8OE-01 U 17755.1 NT Human laminin S 83 chain (LAMB3) gene, exon 13 4700 9812 14909 0.9 1.8OE-01 X92179.1 NT S.tuberosurn mRNA for alcohol dehydrogenase 4977 10080 15150 1.8 1.8OE-01 AW814270.1 EST HUMAN MR3-ST0203-151299-112- 906 ST0203 Homo sapiens cIDNA 5027 10128 15202 4.2 1.80E-01 AF181258.1 NT Mesocrioetus auratus Nataurocholate cotransporting polypeptide mRNA, partial ods 5053 10153 15229 1.3 1.80E-01 A1439881.1 EST_HUMAN 657e04.xl NCI CGAF_Lyrnl 2 Homo saplens cIDNA done IMAGE:2134590 X 5117 10213 1.0 1.8OE-01 AJ000742.1 NT Horno, Saple s hisHl gene, TUTR 563 5725 10841 0.6 1.70E-01 BE385164.1 EST_HUMAN 601274604F1 N1H MGC 20 Homo sapiens cDNA done IMAGE:3615768 5' 788 5939 11091 0.7 1.70E-01 X53330.1 NT P.dumedlil histone gene duste7f- or core histories H2A, H2B, H3 and H4 945 6092 0.6 1.70E-01 P35616 SWISSpROT NEUROFILAMENT TRIPLET L PROTEIN (NEUROFILAMENT LIGHT POLYPEPTIDE) (NIF-Q 1042 6182 11334 0.6 1.70E-01 AF081810.1 NT Lymantria dispar nudeopolyhedrovirus, complete genome 1042 6182 11335 0.6 1.70E-01 AF08181 0.1 NT Lymantria dispar nudeopolyhedrovirus, complete genorns 1938 7055 1.4 1.70E-01 AF255051.1 NT Homo sapiens BN1P3H (BN1P3H) gene, complete ods; nuclear gene for mitochondrial product 2829 7980 13080 1.9 1.70E-01 AF000716.1 NT Vibrio cholerac hypoxanthine phosphodbosyltransferase (hpt) gene, partial cds, hemaggludnin/protease regulatory protein (hapR) gene, complete cds, and YRAL VIBCO gene, partial ods 2829 7980 13081 1.9 1.70E-01 AF000716.1 NT Vibrio cholerac hypoxanthine phosphodbosyttransferase (hpt) gene, partial ods, hernagglutinin/protease regulatory protein (hapR) gene, complete cds, and YRAL VIBCO gene, partial cds 2896 8047 13148 1.7 1.70E-01 AA336909.1 EST HUMAN EST41651 Endometrial tumor Horno sapiens cIDNA 5' end 2967 8118 13225 1.4 1.70E-01 AJ238736.1 NT Naja nala atra ctK-1 gene, exons 1-3 2967 8118 13226 1.4 1.70E-01 AJ238736.1 NT dx-1 gene, exons 1-3 3067 8218 13317 1.9 1.70E-01 AF081514.1 NT Taxus canadensis geranAgeranA diphosphate synthase mRNA, complete cds 3076 8227 13325 2.3 1.70E-01 AB015873.1 NT Ory7Jas latipes mRNA for quanylate cyclase OIGC-Rl, complete cds 3076 8227 13326 2.3 1.70E-01 AB015873.1 NT Ory7Jas latipes mRNA for quanylate cyclase OIGC-RI, complete cds 3557 8696 13794 1.0 1.70E-01 AJ224877.1 NT Horno saplens hael gene, complete CDS 3859 8995 14094 4.4 1.70E-01 AJ235377.1 NT Homo sapiens derivative 11 breakpoint fragment partial intron 10 of the ALL-1 IMLUHRX gene fused to intron 5 of the AF-41FEL gene 4459 9579 1.9 1,70E-01 X52936.11 NT Schistooerca gregaria alpha repetitive DNA 4732 9843 14935 1.4 1.70E-01 A1247635.1 EST HUMAN qh57e09.xl Soare_fetal- tiver-spicen-1 NIFI-S-S1 Homo saplens cIDNA done IMAGE:1 848808 Ysimflar to contains OFR.bl OFR repetitive element; 4986 10089 1.2 1.7012-01 U28376.1 NT Zea mays calclum-dependent protein Winase (MZECDPK2) mRNA, complete ods 5067 10165 1.2 1.70E-01 AF072725.1 NT Zea mays starch branching enzyme lib (ae) gene, complete cds 5319 10449 0,6 1.6OE-01 AF217532.1. NT Horno saplens mevalonate kinase gene, exon 6 and 7 664 7863 10948 0.5 1.6OE-01 R31497.1 EST HUMAN. yh75f12xl Soares placenta NWHIP Horno saplens cDNA done IMAGE:135599 5' R 10953 1.1 1.6OE-01 D84224.1 NT Horno saplens mRNA for methlonyl tRNA synthetase, complete ods 5826 Page 31 of 196 Table 4

1882 7000 12185 0.9 1.6OE-01 P22063 SWISSPROT AXONIN-1 PRECURSOR (AXONAL GLYCOPROTEIN 1941 7058 1,0 1.6OE-01 U10334.1 NT Crassostrea gigas RNA polymerase 11 largest subunit mRNA, partial cds 2247 7353 12560 1.8 1.6OE-01 AF109907.1 NT Homo sapiens S164 gene, partial cds; PSI and hypothetical protein genes, complete cds; and S171 gene, partial ods 2335 7941 12647 1.0 1.60E-01 X94232.1 NT H.sapiens mRNA for novel T-cell activation protein 2443 7542 12750 0.8 1.6OE-01 AB037729.1 NT Homo sapiens mRNA for KIAA1308 protein, partial cels 2863 8014 13115 8.9 1.6OE-01 AF185589.1 NT Horno sapiens cytochrome P450 3A4 (CYP3A4) gene. promoter region 2863 8014 13116 8.9 1.6OE-01 AF185589.1 NT Horno sapiens cytochrome P450 3A4 (CY1P3A4) gene, promoter region 3581 8720 13817 1.3 1.6OE-01 AJ003165.1 NT Populus trichocarpa cv. Trichobel AB13 gene 3581 8720 13818 1.3 1.6OE-01 AJ003165.1 NT Populus trichocarpa cv. Trichobel AB13 gene 3583 8722 0.9 1.6OE-01 A1810954.1 EST HUMAN tu009.xl NCI CGAP Pr28 Homo sapiens cDNA done I MAGE:2250569 3' 4234 9358 14440 7,9 1.6OE-01 AF179680.1 NT Horno sapiens apelin gene, complete cds 4249 9314 14454 4.0 1.6OE-01 A1.163279.2 NT Homo sapiens chromosome 21 segment HS21 C079 4353 9475 2.4 1.6OE-01 AW9686011.1 EST-HUMAN EST380677 MAGE resequences, MAGJ Horno sapiens cIDNA 4361 9483 4.0 1.6OE-01. 6753319 NT Mus musculus chaperonin subunit 3 (gamma) (Cct3), mRNA 4781 9891 14983 0.9 1.6OE-01 Z28330.1 NT S.cerevislae chromosome XI reading frame ORF YKR105c 4781 9891 14984 0.9 1.6OE-01 Z28330.1 NT S.cerevislae chromosome Xi readinm frame ORF YKR105c 4846 9953 15042 7,1 1.60E-01 AF187881.1 NT Homo saplens leukointegdn alpha d gene. promoter and partial cds 4865 9972 15056 1.1 1.60E-01 AA088343.1 EST HUMAN z184h09.s1 Stratagene colon (#937204) Homo sapiens cDNA done IMAGE:511361 Ysimilar to TR:E221955 E221955 38,855 BP SEGMENT OF CHROMOSOME XIV.; 4889 9996 15078 1.9 1.6012-01 AJ006356.1 NT Lycopersicon eiculentum Rsal fragment 2, satellite region 4889 9996 15079 1.9 1.60E-01 AJ006356.1 NT Lycopersicon esculenturn Rsal fragment 2, satellite region 4958 10061 15133 1.1 1.60E-01 BE018707.1 EST HUMAN bb83h08.yl NIH-MGC-10 Homo saplens ciDNA clone IMAGE:3049023 5'similar to gb:M61715 TRYPTOPHANYL-TRNA SYNTHETASE (HUMAN); gb:X69657 M.musculus (MOUSE); 567 5728 10845 0.7 1.50E-01 U34206.1 NT Cricetulus griseus 170 kDa glucose regulated protein (9rp170) mRNA, complete cds 766 5919 11066 1.4 1.50E-01 AL163284.2 NT Homo saplen chromosome 21 segment HS21 C084 1076 6214 11366 0.9 1.50E-01 AJ009735.1 NT Cyprinus carplo mRNA for EGGS22 myosin heavy chain, YUTR 1081 6219 11370 0.6 1.5OE-01 AJ251885.1 NT Homo sapiens partial SLC22A2 gene for organic cation transporter (OCT2), exon 1 1097 6235 0.6 1.5OE-01 1.36125.1 NT Rattus norvegicus insulin-responsive glucose transporter (GLUT4) gene, Vend 1104 6242 11395 1.0 1.5OE-01 Q12697 SWISSPROT PROBABLE CATION- TRANSPORTING ATPASE 9 1198 6330 11485 1.4 1.5OE-01 AW195516.1 EST-HUMAN xn39dl 1.xl NCI CGAP Kidl 1 Horno sapiens cDNA clone IMAGE:2696085 3' 1254 6383 11542 1.0 1.50E-01 D26535.1 NT Human gene for dihydrolipoarnide succAnyitransferase, complete ods (exon 1-15) 1254 6383 11543 1.0 1.50E-01 D26535.1 NT Human gene for dihydrolipoamide sucdnyftmnsferase, complete cds (exon 1-15) 1466 6593 11770 0.5 1.50E-01 AF1 17340.1 NT Mus musculus MAP kinase kinase kinase 1 (Mekkl) mRNA, complete eds 1866 6984 12168 0.6 1.5OE-01 AW444451.1 EST-HUMAN U[-H-B13-akb-b-09-0-Lil. sl NCI-CGAP_Sub5 Homo sapiens cIDNA clone IMAGE:2733641 3' 2999 8151 13251 0.9 1.50E-01 M81441.1 NT Bos taurus factor V variant 2 (factor V) mRNA, complete cds 3013 8165 13266 3.5 1.5OE-01 P01138 SWISSPROT BETA-NERVE GROWTH FACTOR PRECURSOR (BETA-NGF) 8453 13566 4.2 1.50E-01 AA935049.1 EST HUMAN oo68d05.sl NCI-C_GC4 Horno sapiens c[DNA clone IMAGE:1571337 Ysimilar to 3 gb:Ml 1433 RETINOL-BINDING PROTEIN 1, CELLULAR (HUMAN); Page 32 of 196 Table 4 \n C..

3322 8466 13580 0.9 1.50E-01 Z23104.1 NT L.stagnaiis mRNA for G proteincoupled receptor 3322 8496 13581 0.9 1.50E-01 Z23104.1 NT L,stagnalls mRNA for G protein- coupled receptor 3380 8523 13629 1.0 1.5OE-01 AW612237.1 EST-HUMAN hh29f02.xl NCi_WAP-LU24 Homo sapiens cDNA done IMAGE:2956539 Ysimilar to contains element MER16 repetitive element; 3696 883,5 13926 1.3 1.50E-01 U09964.1 NT Mus musculus ICR/SvAss glyceraidehyde 3-phosphate dehydrogenase (Gapd-S) gene, complete cds 3706 8844 13935 185.3 1.5OE-01 7108358 1 Homo sapiens pyruvate dehydrogenase kinase, Isoenzyme 1 (PDK1), nuclear gene encoding NT mitochondrial protein, mRNA 3791 8928 14022 2.4 1.5OE-01 AW665983.1 EST-HUMAN h11 0f06.xl Soares_INIFL_LGI3C S 1 Homo sapiens cDNA done IMAGE:2981411 3, 3806 8943 14038 0.8 1.5OE-01 AJ003165.1 NT Populus trichocarpa cv. Trichobel AB13 gene 3806 8943 14039 0,8 1.5OE-01 AJ003165.1 NT Populus trichocarpa cv. Trichobel AB13 gene 3964 9056 14181 1.0 1.5OE-01 AW366659.1 EST-HUMAN RC2-HT01149-191099-012- c09 HT0149 Homo sapiens cDNA 4006 9137 14219 1.0 1.5OE-01 Z1262C.1 NT B.napus mitochondrion DNA for ORF1 58 4091 9217 14300 8.4 1.50E-01 AL163284.2 NT Homo saplens chromosome 21 segment HS21 C084 4680 9792 14893 1.1 1.5OE-01 BE173796,1 EST-HUMAN CMO-HT0565-280200-245- b10 HT0565 Horno sapiens cDNA 4680 9792 14894 1.1 1.5OE-01 BE173796.1 EST-HUMAN CMO-HT0565-280200-245b10 HT0565 Homo sapiens cDNA 4929 10036 15108 1.3 1.5OE-01 AL1 61560,2 NT Arabldopsis thallana DNA chromosome 4, config fragment No. 60 5072 10170 15248 0.9 1.5OE-01 AF003105.1 NT Arabldopsis thaliana AP2 domain containing protein RAP2.12 mRNA, partial cds 296 5482 0.7 1.40E-01 AF009663.1 NT Homo sapiens T cell receptor beta locus. TCRBV8S5P to TCRBV21S2A2 region 892 6040 0.9 1.40E-01 D78638.1 NT Xenopus laevis mRNA for DNA (cytosine-5- mothAtransferase, complete cds 1238 6367 0.6 1.40E-01 T91864.1 EST HUMAN yd54c0I.sl Soares fetal liver spleen 1INIFI-S Homo sapiens cDNA done IMAGE:1 12032 3' 1714 6837 0.5 1.40E-01 6679980 NT Mus musculus growth differentiation factor 5 (GdfS). mRNA 1717 6840 12019 0.6 1.40E-01 AE001710.1 NT Thennotoga maritima section 22 of 136 of the complete genome 1863 6981 0.9 1.4012-01 AW135741.1 EST-HUMAN Ul-H-Bil-acf-a-09-0-Ul.sl NCI-CGAP-Sub3 Homo sapiens cDNA done IMAGE:2714009 3' 1942 7059 3.7 1.40E-01 AA72061 5.1 EST - HUMAN ny72d07.sl NCI CGAP GCBI Homo saplens cDNA clone IMAGE:1283821 3' 2426 7525 12731 1.0 1.40E-01 P30706 SWISSPROT GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE PRECURSOR (GPAT) 2762 7846 13049 1.1 1.40E-01 A1933496.1 E wm74d01.xl NCI CGAP U1:2 Homo sapiens cDNA done IMAGE:2441665 3' ST-HUMAN 3823 8960 14056 1.0 1.40E-01 R59232.1 EST HUMAN y997a03.rl Soares infant brain I NIB Horno sapiens cDNA done IMAGE:41467 V 3823 8960 14057 1.0 1.40E-01 R59232.1 EST HUMAN yg97a03xl Soares Infant brain 1 NIB Homo sapiens cDNA done IMAGE:41467 9 4083 9210 14294 8.4 1.40E-01 A1699094.1 EST HUMAN tx56c02.xl NCI CGAP Lu24 Horno sapiens cDNA done IMAGE:2273570 3, 4083 9210 14295 8.4 1.40E-01 A1699094.1 EST - HUMAN tx56c02.xl NCI CGAP Lu24 Homo sapiens cDNA done IMAGE:2273570 3' 4144 9270 14349 3.2 1.40E-01 AE001710.1 NT Thermotoga maritime section 22 of 136 of the complete genome 4313 9436 0,8 1.40E-01 AA776287.1 EST-HUMAN zj50b01.sl Soare!_feta(_tiveLspleen-1 NFI.S_SI Horno sapiens cDNA done IMAGE:453673 Ysimilar to gb:X01 057-mal INTERLEUKIN-2 RECEPTOR ALPHA CHAIN PRECURSOR (HUMAN);contains Alu repetitive element; 4803 991 1.2 1.40E-01 AL049866.2 NT Mus musculus chromosome X condgB; X- linked lymphocyte regulated 5 gene, Zinc finger protein 275, Zinc finger protein 92, mmxq28orf 5032 10132 0.9 1.4012-01 AW866022.1 EST HUMAN QV3-SNO022-100500-186-h09 SNO022 Homo saplens cDNA 110212 15291 1,0 1.40E-01 AL1 63284,2 NT - Homo sapiens chromosome 21 segment HS21 C084 Page 33 of 196 Table 4

5147 10242 15316 0.8 1.40E-01 AJ005180.1 NT Lycopersicon esculenturn genomic RAPD band 26 319 5503 10630 0,9 1.30E-01 4758467 NT Homo sapiens G protein-coupled receptor 50 (GPRSO) mRNA 319 5503 10631 0.9 1.3012-01 4758467 NT Horno saplens G protein-coupled receptor 50 (GIPR50) mRNA 518 5682 10803 0.6 1.30E-01 AB013139.1 NT Horno sapions gene for NBSI, complete cds 620 5779 10901 0.9 1.30E-01 AJ277606.1 NT Human calicivirus HUINLVIGiffington/93/UK RNA for capsid protein (ORF2), strain HUINLV/Girlington/93/UK 620 5779 110902 0.9 1.30E-01 AJ277606.1 NT Human calidvirus HUINLV/Gidington/93/UK RNA for capsid protein (ORF2), strain HUINLV/Gidington/93/UK 826 5975 11135 1,1 1.30E-01 X53330.1 NT P.dumeriffi histone gene cluster for core histones H2A, H2B, H3 and H4 876 6024 11184 0.6 1.30E-01 AF139518.1 NT Rattus norvegicus A-kinase anchor.protein mRNA, complete cds 1010 6150 11303 -0.5 1.30E-01 AL1 17078.1 NT Botryfis dnerea strain T4 cDNA library under conditions of nitrogen deprivation 1111 6248 0.8 1.30E-01 ALI 15265.1 NT BoWs dnerea strain T4 cDNA library under conditions of nitrogen deprivation 1426 6553 12221 1.4 1.30E-01 AF146277.1 NT Homo sapiens adapter protein CIVIS mRNA, complete cds 1916 7033 0.9 1.30E-01 AL1 17078.1 NT Botryds dnerea strain T4 cDNA library under nditions of nitrogen deprivation 2124 7234 1,3 1.30E-01 AJ243578.1 NT Rhodopseudomonas addophila pud35, pucAS, pud36, pucA6, pud37, pucA7, pud38, pucA8 and pucC genes and ORF151 2245 7351 1.2 1.30E-01 AW812104.1 EST HUMAN RC4-ST0173-191099-032-d12 ST0173 Homo sapiens eDNA 2329 7432 1.1 1.30E-01 AE001016.1 NT Archaeoglobus fulgidus section 91 of 172 of the complete genome 2542 7638 12838 1.3 1.30E-01 M869118.1 NT Carassius auratus keratin type 1 mRNA, complete cels 3404 8546 13654 1.0 1.30E-01 M21572.11 NT Bovine branched chain alpha- keto acid dihydrolipoyl transacylase mRNA, complete cds 3661 8800 13894 1.2 1.30E-01 AP000001.1 NT Pyrococcus horikoshil OT3 genomic DNA, 1-287000 nt. position (117) 3661 8800 13895 1.2 1.30E-01 AP000001.1 NT Pyrococcus hodkoshii OT3 ienomic DNA, 1-287000 nt. position (117) 3667 8806 13900 0.8 1.30E-01 AB032159.1 NT Horno saplens DD4 gene for dihydrodiol d hydrogenase 4 [AKR 1C4], exon 2 3714 8800 13894 0.9 1.3OE-01 AP000001.1 NT Pyrococcus horikoshil OT3 genomic DNA, 1-287000 nt. p ition(I1/7) 3714 8800 13895 0.9 1.30E-01 AP000001.11 NT Pyrococcus hodkoshil OT3 genomic DNA, 1-287000 nt. position (117) 3734 8871 13963 0.9 1.30E-01 6978840 NT Rattus norvegicus Fibrinogen, gamma polype fide (F99), mRNA 39M06 9W0411 1.7 1.30E-01 AL1 61581.2 NT Arabidopsi thaliana DNA chromosome 4, config fragment No. 77 4046 9173 1.2 1.30E-01 AF020713.1 NT Bacterlophage SPBc2 complete genome 4066 9193 3.4 1.3OE-01 AW364341.1 EST-HUMAN W3-DT0011 8-081299-036-a03 DT001 8 Homo sapiens cDNA 4075 9202 14288 1.9 1.30E-01 AF026805.1 NT Schistosoma mansoni fructose bisphosphate aidolase mRNA, complete cds 4093 9219 14302 16,4 1.30E-01 AW273741.1 EST HUMAN xv23f10.xl Soare_NFL T GBC S1 Homo sapiens cDNA done IMAGE:2813995 3' 4218 9342 14648 1.7 1.30E-01 AL163280.2 NT Horno sapi ns chromosome 21 segment HS21C080 4433 9554 2.2 1.30E-01 BE272339.1 EST HUMAN 601126096F1 NW1 IVIGQ_9 Homo saplens cIDNA done IMAGE:2990063 5' 5074 10172 15250 1.2 1.30E-01 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 5074 10172 15251 1,2 1.30E-01 AL163284.2 NT Homo sa piens chromosome 21 segment HS21C084 5103 10199 15279 0.9 1.30E-01 Z734119.11 NT Mycobacte lurn tuberculosis H37Rv complete genome; segment 571162 378 5584 10717 2.5 1.20E-01 A1421744.1 EST HUMAN C39b02xl NCLCGAF_Bm23 Horno sapiens cDNA done IMAGE:2098539 Ysimilar to gb:U0576Q__mal ANNEXIN V (HUMAN); 5207 0.5 1.20E-01 U66912.1 NT Dictyostellurn discoideurn ORF DG1016 gene, partial cds 56981 0.9 1.20E-01 AF039442.1 NT Horno sapiens colon cancer antigen NY-CO- 45 mRNA, partial cds Page 34 of 196 Table 4

U'? P4 1490 6617 1.1 1.20E-01 AA897474.1 EST HUMAN a148e09.si Soares NFLJ_GBC_9.1 Homo sapiens cDNA bone IMAGE:1460584 3'similar to TR:C116671 Q16671 ANTI-MULLERIAN HORMONE TYPE 11 RECEPTOR PRECURSOR.; 1613 6739 11909 1.2 1.20E-01 014934 SWiSSPROT NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 4 IT CELL TRANSCRIPTION FACTOR NFAT3) (NF-ATC4) (NF-AT3) 1631 6757 11928 1.0 1.20E-01 A1285402.1 EST HUMAN qt69109.xl NCI CGAP Eso2 Homo sapiens cDNA clone IMAGE:1960553 3' 1730 6853 11.0 1.20E-01 X8921 1.1 NT H.sapiens DNA for endogenous retroviral like element 1739 6862 0.6 1.20E-01 U41418.1 NT Vibrio fumissli chitodextrinase gene, complete ods 1877 6995 0.5 1.20E-01 AW449368.1 EST HUMAN Ul-H-B13-aki-c-1 0-0-Ul.sl NCI-C_SubSH.omo sapiens cDNA clone IMAGE:2734554 3' 2240 7346 12557 1.0 1.20E-01 AL163213.2 NT Homo sapiens chromosome 21 segment H521C013 2546 7642 12842 0.7 1.2OE-01 AW996556.1 EST-HUMAN OV3-BNOO4r)-220300-129- flO BN0046 Homo sapiens cDNA 2697 7783 12983 0.9 1.20E-01 A1623388.1 EST-HUMAN ts18907.xl NCI CGAP Panll Homo sapiens cDNA done IMAGE:2228988 Ysimilar to TR:014048 Q1048 C5LAGEN VI ALPHA.2 ALTERNATIVE C-TERMINAL DOMAIN. [1) xontains element PTR5 repetitive element; 2812 7963 13069 1.5 1.20E-01 U18018.1 NT Human E1 A enhancer bEding protein (E1A-F) mRNA, partial cds 2872 8023. 13126 2.0 1.20E-01 A1720470.1 EST-HUMAN as80c09.xl Barstead colon HPI-RI37 Horno saplens cDNA clone IMAGE:2335024 3'sImilar to gb.105095 60S RIBOSOMAL PROTEIN L30 (HUMAN); 2904 8OF 13160 2,9 1.20E-01 M16364.1 NT Human creadne kinase-B mRNA, complete ods 2974 8125 13232 1.0 1.20E-01 X56882.1 NT Wheat mRNA for a group 3 late embryogenesis abundant rotein (LEA) 3177 8326 13438 2.0 1.20E-01 M86376.1 NT Rat tropoelastin gene, exon 28- 36 3177 8326 13439 2.0 1.20E-01 M86376.1 NT Rat tropoelastin gene, exon 28- 36 3192 8341 13455 2.0 1.20E-01 AW370668.1 EST-HUMAN QV1-BT0259-26109M21-d05 BT0259 Homo saplens cIDNA 3219 8367 1.0 1.20E-01 U67600.1 NT Methanococcus jannaschh section 142 of 150 of the complete genome 3433 8575 0.8 1.20E-01 Z991 18.1 NT Bacillus subtifis complete genome (section 15 of 2l): from 2795131 to 3013540 3477 8618 13730 1,1 1.20E-01 X56882.1 NT Wheat mRNA for a group 3 late embryogenesis abundant protein (LEA) -3477 618 13731 1,1 1.20E-01 X56882.1 NT Wheat mRNA for a grouR 3 late embTogenesis abundant protein (LEA) 3554 8575 1.2 1.20E-01 Z991 18.1 NT Bacillus subtills comelete genome (section 15 of 2l): from 2795131 to 3013540 4090 9216 14298 2,2 1.2OE-01 Z54255.1 NT P.ciarkii mRNA; repeat region (11) 2MRT7) 4090 9216 14299 2.2 1.20E-01 Z54255.1 NT P.clarkii mRNA; repeat region (11) 2MRT7) 4745 9856 14948 7.1 1.20E-01 S65019.11 NT mucin [rats, Sprague-DavAey, sulfur-dioxide-treated tracheal epithelium, mRNA Partial, 390 nt] 4775 9885 14977 6.7 1.20E-01 Z32774.1 NT H.sapiens gene for N-methy]-D- aspartate receptor R1 exons 6-21 4775 9885 14978 6.7 1.20E-01 Z32774.1 NT H.saplens gene for N-methyl-D- aspartate receptor R1 exons 6-21 5012 10114 1.0 1.20E-01 P16466 SWISSPROT HEMOLYSIN PRECURSOR 5174 10268 15339 1.5 1.20E-01 010441 SWISSPROT HYPOTHETICAL 52.4 KI) PROTEIN C12810.08C.IN CHROMOSOME 1 5174 10268 15340 1.5 1.20E-01 Q10441 SWISSPROT HYPOTHETICAL 52.4 KD PROTEIN C12B10.08C IN CHROMOSOME 1 5199 10290 2.5 1.20E-01 AW401836.1 EST HUMAN Ut-HF-BKO-aah-d-01-0-Ul.rl NIH-MGC-36 Homo saplens dDNA clone IMAGE:3053617 5, 5521 57151 10833 1.0 1.1OE-01 A1561003.1 EST HUMAN tn18d08.xl NCI CGAP Bm25 Homo sapiens cDNA done IMAGE:2167983 3' 7_ 1 Page 35 of 19d Table 4

599 5757 10876 1.2 1.1OE-01 AA569006.1 EST HUMAN nmO8gll.sl NCI CGAP_ColO Homo sapiens cDNA done IMAGE:1059620 3'similar to gb:X06985-mal'HEME OXYGENASE 1 (HUMAN); 1069 6207 1.3 1.1OE-01 ALI 61560,2 NT Arabidopsis thaliana DNA chromosome 4, cbndg fragment No. 60 1143 7908 11431 1.4 1.1OE-01 AW972158.1 EST HUMAN EST384142 MAGE resequ!nces, MAGL Horno saplens cDNA 1229 6359 11522 0.6 1.1OE-01 D64004.1 NT Synechocysts sp, PCC6803 complete genome, 23127. 2868767-3002965 1821 6940 0.9 1.1OE-01 AF051784.1 NT Xenopus laevis 14S cohesin SIVIC1 subunit mRNA, complete cds 2266 7372 0A 1.1012-01 6755215 NT Mus musculus pre T-cell antigen receptor alpha (Ptera). mRNA 2492 7875 1.2 1.10FE-01 6978676 NT RattOs norvegicus Procollagen 11 alpha 1 (Co12al). mRNA 2520 7616 1.2 1.1OE-01 AW821909.1 EST HUMAN RCO-ST0379-210100-032-904 ST0379 Horno sapiens cIDNA 2825 7976 13078 1.8 1.1OE-01 S82418.1 NT intedeukin-12 p35 subunit [mice, GenomIc, 700 nt, segment 4 of 5) 2997 8149 13249 0.8 1.1OE-01 F03265.1 ESTHUMAN HSC1 RF022 normalized infant brain cDNA Homo sapiens cDNA clone c-1 ri02 3' 3299 8445 1.4 1.1OE-01 6753231 NT Mus musculus calcium channel, voltage- dependent, T type, alpha 1 G subunit (Cacnal g), mRNA 3374 8517 13624 3.0 1.1OE-01 BE393186.1 EST HUMAN 601308679F1 NIH MGC 44 Homo sapiens cIDNA done IMAGE:3627066 5' 3405 8547 13655 1.5 1.1OE-01 X62135.1 NT C.reinhardbi nuclear gene on linkage group XIX 3534 8675 13778 0.9 1.1OE-01 Y07695.1 NT A.Immersus gene for transposase 3648 8787 13880 1,2 1.1OE-01 X52708.1 NT G.9allus ge a encoding non- histone chromosomal protein HIVIG-14b, exons 4 and 5 4021 9150 14230 1.3 1.1OE-01 AW819412.1 EST-HUMAN MR3-ST0290-290100-025- g07 ST0290 Homo sapiens cIDNA 4021 9150 14231 1.3 1.1OE-01 AW819412.1 EST HUMAN MR3-ST0290-290100-02"07 ST0290 Homo sapiens cDNA 4027 9155- 0,9 1.1OE-01 AF030001.1 NT Mus musculus major histocompatibility locus class Ill region:butyrophilin-like protein gene, partial cds; Notch4, ' P13X2, RAGE, lysophaddic add acyl transferasealpha, palmitoyl-protein thioesterase 2 (PPT2), CREB-RP, and tenascin X (TNX) genes, comple> 4159 9285 7.9 1.1OE-01 AF157066.1 NT Drosophila melanogaster klarsicht protein (War) mRNA, complete cds 4189 9315 14399 0.8 1.1OE-01 AW802056.1 EST HUMAN IL5-UM0070-020500-068- a08 UM0070 Homo sapiens cDNA 4341 9464 5.9 1.1OE-01 P14728 SWISSPROT HYPOTHETICAL 82 KI) AVIRULENCE PROTEIN IN AVRBS3 REGION 4466 9586 14682 0&99 1.1OE-01 AF064564.2 NT Fugu rubripes nourofibromatosis type 1 (NFl), A-kinase anchor protein (AKAP84), BAW protein (BAW), and WS131 protein (WSB1) genes, complete cds 4539 9658 14746 2.0 1.1OE-01 S44957.1 NT Tapa-1 =integral membrane protein TAPA-1 [mice, B cell lymphorna line 38C13, Genomic, 1973 nt, segment 1 of 7] 4725 9837 14929 1.3 1.1OE-01 Y07695.1 NT A.Immersus gene for transposase 4819 9928 15019 1.3 1.1OE-01 D90908.1 NT Synechocysfis sp. PCC6803 complete genome, 10127, 1188886-1311234 4979 10082 15152 1.1 1.1OE-01 Z83335.1 NT S.pneurnonlae dexB, capl[A,B,C, D,E,F,G,H,I,J,K] genes, dTDP-rhamnose biosynthesis genes and aliA gene 1183 6316 1.4 1.OOE-01 062855 SWISSPROT DEOXYRIBONLICLEASE 11 PRECURSOR (DNASE 11) (ACID DNASE) (LYSOSOMAL DNASE 11) 1251 6380 11539 0.6 1.OOE-01 A1985499.1 EST-HUMAN wsOBdOl.xl NCI_PGAP_Ndl 1 Homo sapiens eDNA clone IMAGE:2496577 Ysimilar to contains MER74 MER7 repetitive element., 1336 6464 11632 10 1.OOE-01 AE001492.1 NT Hellcobacter pylod, strain J99 section 53 of 132 of the complete genome 1370 6498 11664 1.0 1.OOE-01 AL161504.2 NT Arabidopsis thallana DNA chromosome 4. contig fragment No. 16 7026 12215 0,9 1.OOE-01 AW862179.1 EST HUMA MR1 -CT0355A 80200-007-907 CT0355 Homo sapiens cDNA _ Page 36 of 196 Table 4 rC.4 2439 7538 12745 1.0 1.0OE-01 AW451365.1 EST-HUMAN Ul-H-B13,aicd-07-0-01. sl NCI-CGAP-Sub5 Homo sapiens eDNA done IMAGE:2736420 3' 3130 8279 13386 4.7 1.OOE-01 AA314107.1 EST HUMAN EST185958 Colon carcinoma (HCC) cell line 11 Homo sapiens cDNA Vend 3130 8279 13387 4.7 1.OOE-01 AA314107.1 EST-HUMAN EST185958 Colon carcinoma HCC) cell line 11 Homo sapiens cDNA S' end 3603 8742 13837 2.6 1.OOE-01 U76890.1 NT Human amelogenin (AMELX) gene on X chromosome, pard 1 promoter region 4307 9430 14519 1.5 1.OOE-01 AE002265.2 NT Chlamydophila pneumonlae AR39, section 91 of 94 of the complete genome 4455 9575 1.2 1.0OE-01 A1792349.1 EST-HUMAN an32c04.y5 Gessler Wilms tumor Honio saplens cDNA done IMAGE:1 700358 5 4613 9727 14821 1.3 1.0OE-01 U50450.1 NT Drosophila melanogaster tyrosine kinase p45 lsoform (fer) mRNA, complete cds 4838 9945 15034 2.1 1.0OE-01 AW952344.1 EST-HUMAN EST364414 MAGE resequencesi MAGB Homo saplens cDNA 4983 10086 15156 6.0 1.OOE-01 U50133.1 NT Human ankydn (ANK1) gene, exon 42 and complete cds 4983 10086 15157 6.0 1.OOE-01. U50133.1 NT Human ankyrin (ANK1) gene, exon 42 and complete ods 5149 10244 0.8 1.0OE-01 BE157114.1 EST HUMAN RC3-HT0371-180200-015-c01 HT0371 Homo sapiens cDNA 5158 10253 15324 0,9 1.OOE-01 D49683.1 NT Mouse FTZ-Fl gene 213 5406 1.6 9.9OE-02 AF136389.1 NT Bacillus cereus strain H6 gyrase B subunit (gyrB) gene, partial ods 3229 8377 13484 1.2 9.9OE-02 AF099810.1 NT Homo sapiens neureyJn Ill- alpha gene, partial ods 551 5714 0.5 9.8OE-02 X56338.1 NT 0.sadva RAmy3C gene for alpha-amylase 1711 6834 12014 0.6 9.8OE-02 4503224 NT Homo saplens cytochrome P450, subfamily I1F, polypepfide 1 (CYP2F1) mRNA 3106 825613359 3.3 9.8OE-02 AF184274.1 NT Daucus carota leucoanthocyanidin dioxygenase 2 (LDOX) mRNA, LDOX-2 allele, complete cds.

4131 9257 14337 6.7 9.8OE-02 AF257329.1 NT Leptosphaeda maculans betatubulin mRNA, complete eds 4131 9257 14338 6.7 9.8OE-02 AF257329.1 NT Leptosphaeria maculans beta- tubulin mRNA, complete cds 1328 6457 11624 1.2 9.70E-02 ABOO5808.1 NT Aloe arborescens mRNA for NADP- malic enzyme, complete cds 1567 6693 1.0 9.70E-02 4503710 NT Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFIR3) mRNA 2214 7322 12536 0.9 9.70E-02 BE168660.11 EST HUMAN QV1-HT0516-070300-095- a04 HT0516 Horno sapiens cDNA 3901 9036 3.6 9.70E-02 Q99795 SWISSPROT CELL SURFACE A33 ANTIGEN PRECURSOR (GLYCOPROTEIN A33) 1969 7084 12285 1.3 9.60E-02 A1080721.1 EST-HUMAN oz47dl 1.xl Soares Nhi- IMPu S1 Homo sapiens cDNA done IMAGE:1678485 3' 1969 7084 12286 1.3 9.6OE-02 A1080721.1 EST HUMAN oz47dl 1.xl Soares Nhi- IMPu S1 Homo saplens cDNA done IMAGE:1678485 3' 2250 7356 12563 1.0 9.6012-02 AW836565.11 EST HUMAN PM3-LT0032-030100-004- b01 LT0032 Homo sapiens cDNA 2250 7356 12564 1.0 9.6OE-02 AW836565.1 EST HUMAN PM3-LT0032-030100-004- b01 LT0032 Homo saplens cDNA 4248 9 3 14453 6.0 9.60E-02 Z32686.2 NT Proteus mirabilis flmbdai operon, strain H14320 4932 10038 15111 0.9 9.6OE-02 AW966230.1 ESTHUMAN EST378303 MAGE resequences, MAGI Homo saplens cDNA 5093 10191 15270 0.8 9.6012-02 BE061729.11 EST-HUMAN RC5-13TO254-031099- 01 1 -a03 BT0254 Homo saplens CDNA 4012 9143 14226 2.3 9.5OE-02 AW992395.1 EST HUMAN CM2-BN0023-050200-087- f12 BN0023 Homo saplens cDf4A 5062 10160 15236 0.9 9.5OE-02 U63374.1 NT Lycopersicon esculentum polygalacturonase isoenzyme 1 beta subunit gene, complete cds 1820 6939 12129 0.9 9.4OE-02 U55944.11 NT Cavia porcellus 3beta- hydroxysterold sulfotransferase mRNA, complete cds 1820 6939 12130 0.9 9.40E-02 U55944.1 NT Cavia porcellus 3betahydroxysteroid sultotransferase mRNA, complete cds 3M 8941 14036 4.3 9.40E-02 Z33059.1 NT M.capricolum DNA for CONTIG MC073 4980 10083 15153.9 9.40E-02 6753517 NT Mus musculus coding region detenninant-binding protein (Crdbp), mRNA 8111 1.8 9.3OE-02 4809280 NT Homo sapiens BAll-assodated protein 3 (BAIAIR3) mRNA Page 37 of 196 Table 4

C.4 2993 8145 5.4 9.30E-02 6912525 NT Homo sapiens nasopharyngeal epithelium spedfic protein 1 (NESGI), mRNA 3051 8202 13303 2.4 9.30E-02 L78810.1 NT Homo sapiens ADPIATP carrier protein (ANT-2) gene, complete cds 3226 8374 13482 1.2 9.30E-02 Y08582.1 NT F.rubdpes hsp70-5 gene, 6 4060 9187 14270 3.5 9.30E-02 BE391943.1 EST-HUMAN 601286082F1 N1H MGC 44 Horno saplens cDNA done IMAGE.3607653 V 4060 9187 14271 3,5 9.30E-02 BE391943.1 EST HUMAN 601286082F1 N1H MGC 44 Homo sapiens cIDNA done IMAGE:3607653 5' 228 5419 10540 1.5 9.20E-02 U603115.11 NT Molluscum contagiosurn virus subtype 1, complete genome 228 5419 10541 1.5 9.20E-02 U603115.1 NT Molluscurn contaglosum virus subtype 1, complete genome 228 5419 10542 1.5 9.2012-02 U60315.11 NT Molluscum contaglosum virus subtype 1, complete genome 2180 7289 0.8 9.20E-02- R54156.1 EST HUMAN yg98f07.rl Soares Intantbrain INIB Homosapiens cDNAcione IMAGE:41618 5, 3142 8291 13398 4.5 9.20E-02 028631 SWISSPROT MAJOR EPIDIDYMIS-SPECIFIC PROTEIN E4 (EPIDIDYMAL PROTEIN BE-20) 3265 8412 13518 1.2 9.20E-02 AA534354.1 EST-HUMAN nf79e01.sl NCI CGAP Co3 Homo sapiens cDNA done IMAGE:926136 3 3537 8678 1.1 9.20E-02 6755215 NT Mus musculus pre T-cell antigen receptor alpha (Ptcra), mRNA 4145 9271 1.3 9.20E-02 U92048.1 NT Human erpesvirus 1 strain KOS-63, latency-associated transcript, promoter region 4211 9335 1,0 9.20E-02 BE299722.11 EST-HUMAN 600944365F1 NIH MGC 17 Homo saplens cDNA done IMAGE:2960176 5, 4538 9657 14745 1.9 9.20E-02 X96402.1 NT G.gallus Mia-CK gene 417 5206 10308 0.9 9.1OE-02 X77665,1 NT 0. cuniculus k12 keratin gene 2365 7467 12671 1.0 9.1012-02 P78985 SWISSPROT 6-PHOSPHOFRUCTOKINASE (PHOSPHOFRUCTOKINASE) (PHOSPHOHEXOKINASE) 3618 8757 1.1 9.1OE-02 AW372569.1 EST-HUMAN PM2-13T0349-161299-001402 BT0349 Horno saplens cDOA 3959 10298 14175 1.1 9.1OE-02 P16415 SWISSPROT ZINC FINGER PROTEIN ZFP-36 3959 10298 14176 1.1 9.1OE-02 P16415 SWISSPROT ZINC FINGER PROTEIN ZFP-36, 3989 9121 14205 1,6 9.1OE-02 P42526 SWISSPROT HISACTOPHILIN 2 (HISTIDINE- RICH ACTIN-BINDING PROTEIN 2) (HS 11) 4383 9505 14590 1.8 9. 1 OE-02 AL161554.2 NT Arabidopsis thatiana DNA chromosome 4, contig fragment No. 54 727 5881 11022 1.2 9.OOE-02 P15328 SWISSPROT FOLATE RECEPTOR ALPHA PRECURSOR (FR-ALPHA) (FOLATE RECEPTOR 1) (FOLATE RECEPTOR, ADULT) (ADULT FOLATE-BINDING PROTEIN) (FBP) (OVARIAN TUMOR-ASSOCIATED ANTIGEN MOV18) (KB CELLS F13P) 1617 6743 11911 2.3 9.OOE-02 BE220482.1 EST-HUMAN hv39g10.xl NCI_PGAP- LU24 Homo sapiens cDNA done IMAGE:3175842 3'similar to contains Alu repetitive element; 2772 7856 13060 1.1 9.0012-02 AF138522.11 NT HIV-1 p13c095-06 from USA envelope glycoprotein (env) gene, partial cds 2772 7856 13061 1.1 9.OOE-02 AF1138522.1 NT HIV-1 P8c095-06 from USA envelope glycoproteln (env) gene, partial cds 3294 8440 13550 0.9 9.OOE-02 AF279135.1 NT Dictyostellum discoldeum spore coat structural protein SP65 (cotE) gene, complete cds 4202 9327 14415 0.8 9.OOE-02 S68757.1 NT corticosterold-binding globulin [Saimirl sclureus=squirrel monkeys, liver, mRNA, 1474 nt) 4202 9327 14416 0.8 9.OOE-02 S68757.11 NT corficosteroid-binding globulin [Saimid sciureus=squirrel monkeys, liver, mRNA, 1474 nil 4321 9444 14533 1.2 9.OOE-02 P55268 SWISSPROT LAMININ BETA-2 CHAIN PRECURSOR (S-LAMININ) 4568 9686 14780 1.8 9.OOE-02 X65740,2 NT Plasmodium faldparum P-type ATPase 3 gene 5142 10237 15312 1.1 9.OOE-02 Q24597 SWISSPROT REGULATORY PROTEIN ZESTE 5151 10246 15318 1.0 9.OOE-02 BE159422.1 EST HUMAN MRO-HT0407-140200-009- b04 HT0407 Homo saplens eDNA 2338 7440 12649 0.5 8.9OE-02 BE153572.1 EST HUMAN PMO-HT0339-251199-003- d01 HT0339 Homo saplens cDNA 9653 14740 0.9 8.9OE-02 AA424887.1 EST HUMAN zw03d04.sl Soares Nhi- IMPu_SI1 Homo sapiens cDNA clone IMAGE:768199 3' 45314 6479 11645 1.3 8.8OE-02 027474 SWISSPROT PROBABLE DNA LIGASE (POLYDEOXYRIBONUCLEOTIDE SYNTHASE [ATP]) Page 38 of 196 Table 4

3828 8965 14064 1.0 8.8OE-02 AA299128.11 EST-HUMAN EST1 1595 Uterus Horno sapiens cDNA W end 3948 9081 3.2 8.8OE-02 000268 SWISSPROT TRANSCRIPTION INITIATION FACTOR THID 135 KDA f UBUNIT (TAF11-135) (TAF11135) (TAF11-130) (TAF11130) 4205 9330 1.1 8.8OE-02 4580423 NT Homo sapiens paired box gene 6 (aniddia, keradds) (PAX6), isoform b, mRNA 3636 8775 13869 3.0 8.70E-02 U82695.2 NT Horno sapiens zinc finger protein 92 (ZFP92), expressed-Xq28STS protein (XQ28ORF), and biglycan (BGN) genes, complete cds; and plasma membrane calcium ATPase isoform 3 (PMCA3) gene, partial ods 3636 8775 13870 3.0 8.70E-02 U82695.2 NT Homo saplens zinc finger protein 92 (ZFP92), expressed-Xq28STS protein (X028ORF), and biglycan (BGN) genes, complete cds; and plasma membrane calcium ATPase isoform 3 (PMCA3) gene, partial cds 3879 9015 14110 0.8 8.70E-02 W87841.1. zh68a02xl Soares-fetal-liver - spleen-1 NFLM1 Homo sapiens cDNA clone EST HUMAN IMAGE:417194 5' similar to contains element MER12 repetitive element; 4609 9723 14818 1.2 8.70E-02 AF1178636.1 NT Mus musculus JNK interacting protein-3a (Jip3) mRNA, complete cds 5034 10134 1.1 8.70E-02 AEwb895.1 NT Methanobactedum thermoautotrophicum from bases 1176181 to 1189406 (section 101 of 148) of the complete genome 1232 6361 11523 2.4 8.60E-02 AJ271736.1 NT Homo sapiens Xq pseudoautosomal region; segment 2/2 2197 7306 12518 0.8 8.60E-02 BE408667.1 EST-HUMAN 601304016F1 NIH MGC 21 Homo sapien$ cDNA done IMAGE:3638643 5' 3151 8300 13408 2.9 8.60E-02 L05468.1 NT Trichomonas vaginalis beta- tubulin (btubl) gene, complete cds 3264 8411 0.8 8.6OE-02 AJ277590.1 NT Dictyostellum discoldeum 1p1A gene for Inositol 1,4,5-trisphosphate receptor-like protein (1p1A), exons 1-4 3593 8732 3.1 8.6012-02 AF153362.1 NT Dictyostelium discoldeum adenylA cyclase (acrA) gene, complete cds 4385 9507 14592 0.9 8.60E-02 U68179.1 NT Oryctolagus cuniculus galectin-3 gene, untranslated exon and Wflanking region 1330 6459 11625 0.6 8.5OE-02 AB007855.1 NT Homo sapiens KIAA0395 mRNA, partial cds 2347 7449 12656 1.0 8.5OE-02 AE000652.1 NT Helicobacter pylorl 26695 section 130 of 134 of the wmplete'genome 2632 7950 12923 1.5 8.4OE-02 W69330.11 EST HUMAN zd44el 1.rl Soares_fetaLhearLNbHH19W Homo sapiens cDNA done IMAGE:343532 5' 4152 _9278 0.8 8.40E-02 AA847045.1 EST-HUMAN oe09b09.sl NCLWAP-Ov2 Homo saplen,s cDNA done IMAGE:1 385369 similar to contains Alu repetitive element;contains element L1 repetitive element 1965 7081 12280 0.9 8.3OE-02 5835680 NT Ixodes hexagonus mitochonddon, complete genom e 1965 7081 12281 0.9 8.30E-02 5835680 NT Ixodes hexagonus mitochondrion, complete genome 3544 8685 13786 6.2 8.30E-02 P75334 SWISSPROT HYPOTHETICAL LIPOPROTEIN MG309 HOMOLOG PRECURSOR 3567 8766 13803 0.9 8.30E-02 A1436797.1 EST HUMAN th82g06.xl Soare_NhHMPu_Sl Homo sapiens cDRA done IMAGE:2125210 3, 3567 8706 13804 0.9 8.30E-02 A1436797.1 EST-HUMAN th82q06.xl Soares Nhi- IMPu S1 Homo sapiens cDNA done] MAGE:2125210 3' 1243 6372 0.7 8.20E-02 6681050 NT Mus musculus colony stimulating factor 3 receptor (granulocyte) (Csf3r), mRNA 1357 6485 2.8 8.20E-02 Y08170.2 NT Gallus gallus mRNA for for OBCAM protein gamma Isoform 1480 6607 11780 1.2 8.20E-02 AF167077.2 NT Canis fmillads glutamate transporter (EAAT4) mRNA, complete cds 3036 8188 1.8 8.20E-02 AL1 63206.2 NT Homo sapiens chromosome 21 segment HS21 C006 3733 8870 1.3 8.20E-02 AL1 61498.2 NT Arabidopsis thallana DNA chromosome 4, config ftagment No. 10 3926 9060 14147 1.1 8.20E-02 AL163206.2 NT Homo sapiens chromosome 21 segment H821C006 4187 9313 14394 5.4 8.2012-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR 9313 14395 5.4 8.2012-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR Page 39 of 196 Table 4

0 4187 9313 14396 5.4 8.20E-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR 5014 10116 15187 2.8 8.20E-02 U76009,1 NT Mus musculus zinc transporter (ZnT-3) gene, complete cds 1479 6606 11779 1.7 8.1 OE-02 AB017138.1 NT Pseudomonas pudda malonate decarboxylase gene duster (mdcA, mdcB, mdcC, mdcD, mdcE, mdeG, mdcH, mdcL and mdcM genes), complete ods 7879 10316 1.1 8.OOE-02 AW954653.1 EST-HUMAN EST366723 MAGE resequences, MAGC Homo sapiens cIDNA 920 6066 11222 1.3 8.OOE-02 U60315.1 NT Molluscum contagiosum virus subtype 1, complete gienome 1353 6481 11647 4.6 8.OOE-02 D63861.1 NT Homo sapiens DNA for cyclophilin 40, complete cds 1671 7923 11972 3.8 8.OOE-02 D26535.1 NT for dihydrolipoamide succinyitransferase, complete eds (exon 1-15) 1671 7923 11973 3.8 8.OOE-02 D26535.11 NT Human gene for dihydrolipoamide succinyitransferase, complete cds.(exon 1-15) 1861 6979 12164 1.4 8.OOE-02 BE067219.1 EST HUMAN PM3-BT0347-170200-001b08 BT0347 Homo sapiens cIDNA 2323 7426 12635 1.0 8.OOE-02 D90915.1 NT Synechocystis sp. PCC6803 complete genome, 17127, 2137259-2267259 2323 7426 12636 1.0 18.OOE-02 D909115.1 NT Synechocysfis sp. PCC6803 complete genome, 17127, 2137259-2267259 2790 6213 11365 0.8 8.OOE-02 M23449.11 NT Dictyoseflum discoideum 9dic nudeofide phosphodiesterase gene, complete cds 3745 8883 13972 0.8 8.OOE-02 AW966118.1 EST HUMAN EST378191 MAGE resequences, MAGI Homo sapiens cIDNA 3980 9112 1.1 8.OOE-02 4503034 NT Homo sapiens cAMP responsive element binding protein-like 2 (CREBL2) mRNA 4709 9821 5.6 8.OOE-02 X72794.1 NT M.musculus gene for gelafinase B 4834 9942 15031 0.9 8.OOE-02 M28071.1 NT Herpesus salmirl transformatonassociated protein (STP), and dihydrofolate reductase (DHFR) gene,s complete cds, and small nuclear RNAs (uRNAs) 1544 6671 11841 0,5 7.9OE-02 AE001501.1 NT Hellcobacter pylod, strain J99 section 62 of 132 of the complete genome 1544 6671 11842 0.5 7.9OE-02 AE001501.1 NT Helicobacter pylod, strain J99 section 62 of 132 of the complete genome 2127 7237 12445 1.4 7.9012-02 BE250008.1 EST-HUMAN 600943191F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE:2959510 5' 2948 8099 13205 6.4 7.9OE-02 A1582029.1 EST-HUMAN ar98c08.xl Barstead colon HPI-RB7 Homo sapiens cDNA done IMAGE:2173646 3'simiiar to gb226876 60S RIBOSOMAL PROTEIN L38 (HUMAN); 3255 8402 13508 1.2 7.9OE-02 A1457925.1 EST-HUMAN 455g03.xl Soares-NSF. _FCL9W_OT_PP_P_Sl Homo sapiens cDNA clone IMAGE:2145460 3' 3776 8913 14003 3.3 7.9OE-02 6681044 NT. Mus musculus colony stimulating flactor 1 receptor (Osh r). mRNA 3776 8913 14004 3.3 7.9OE-02 6681044 NT Mus musculus colony stimulating factor 1 receptor (Csflr), mRNA 4706 9818 1.2 7.9OE-02 ABOO8019.1 NT' Arabidopsis thaliana RXW24L mRNA, partial cds 1192 6324 11479 1.4 7.8012-02 A1793275.1 EST-HUMAN oo59c102.y5 NCI CrpAP LuS Homo sapiens c[DNA done IMAGE:1 570467 5, similar to contains Ll.t3 Ll repedive element; lilar to 1192 6324 11480 1.4 7.8OE-02 A1793275.1 EST HUMAN oo59d02.y5 NCI-CGAP-Lu5 Homo sapiens cIDNA clone IMAGE:1570467 Csimilar to contains Ll.t3 Ll repetitive element., 1378 7915 11671 1.0 7.8OE-02 AW866884.1 EST HUMAN CM3-SN0032-010400- 137407 SNO032 Homo sapiens cIDNA 5019 8828 2.7 7.8011-02 BE250048.1 EST-HUMAN 600943055F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE:2959693 5' 5197 10288 15357 1.0 7.8OE-02 A1418520.1 EST HUMAN tg48912.xl Soares - NFL T - GBC - S1 Homo sapiens c1DNA done IMAGE:2112070 Ysimilar to contains MER1O.t3 ME1i10 repetitive element; 3538 8679 2.1 7.7OE-02 AJ238093.1 NT Homo sapiens partial AF-4 gene, exons 2 to 7 and Alu repeat elements 3362 8505 13613 0.9 7.6OE-02 AA296447.1 EST HUMAN EST1 12214 Cerebellum 11 Homo sapiens ciDNA 5' end similar to similar to protocadherin 43 351' '8653 13761 0.9 7.6012-02 AJ400877.1 NT Homosapiens ASCL3 gene, CEG121 gene, C11orl14gene, C11orl15gene, C11orl16gene and Cl 1 orfl 7 gene Page 40 of 196 Table 4

4765 9876 1.0 7.6OE-02 AW858844.1 EST HUMAN RC3-CTOW-1 10300-014-a05 CTO347 Homo sapiens cIDNA 6 (neurotransmitter transporter, glycine), member 9 Homo sapiens solute carrier family 767 5920 11067 1.1 7.5OE-02 5902093 NT (SLC6A9), mRNA Homo sapiens solute carrier family 6 (neurotransmitter transporter, glycine), member 9 767 5920 11068 1.1 7.5OE-02 5902093 NT (SLC6A9), mRNA 1878 6996 12181 0.9 7.5OE-02 AL163278.2 NT Homo saplens chromosome 21 segment HS21C078 4407 9527 14617 0.8 7.5OE-02 AB015961.1 NT Homo sapiens IL-18 gene for Interleukin-18, intron, 1 and exon 2 00-01 1-HO9 LT0054 Homo sapiens cIDNA 469 10763 1.2 7.4OE-02 AW838547.1 EST HUMAN RCS-LT0054-2601 1444 6571 1,1 7.4OE-02 AF030027.1 NT guine herpesvirus 4 strain NS80567, complete genome homeodomain transcription factor 1 (Phl), mRNA 2536 7632 0,9 7.4OE-02 6755069 NT Mus musculus paired-like TGBC SI Homo sapiens cDNA done IMAGE:2358385 3' 3545 -6-86 13787 0.9 7.4OE-02 A1807885.1 EST HUMAN W43h01.xl Soares NFL complete cds ADPIATP carrier protein (ANT-2) 4606 ---9720 14814 3.4 7.4OE-02 L7881 0.1 NT Homo sapiens gene, like kinase 1 (Acvdl), mRNA Activin 4687 9799 14902 2.7 7.40E-02 6978442 NT Rattus norvenicus receptor c-terminal hydrolase related pollypeptide (Uchrp), mRNA 4858 9965 15050 1.7 7.4OE-02 6678492 NT Mus musculus ubiquinfin section 101 of 136 of the complete genome 669 5825 10952 0.9 7.3OE-02 AE001789.1 NT Thermotoga maritima NN1004 Homo sapiens cUNA 1465 717 11769 1.1 7.3OE-02 AW900281.1 EST HUMAN CM0-NW004-130300-284-908 1801 7927 5.4 7.3OE-02 A1-163302.2 NT Homo sapiens chromosome 21 segment HS21 Cl 02 factor USF2 (USF2) gene, exons 8-10 and complete ods 4927 10034 1.0 7.3OE-02 U12283.1 NT Mus musculus transcription 927 thermoautotrophicum from bases 1029155 to 1039934 (section 88 of 148) Methanobacterium 11 4 5315 10443 __0.9 7.2012-02 AE000882.1 NT of the complete genome 114 Methanobacterium thennoautotrophicum from bases 1029155 to 1039934 (section 88 of 148) 114 5315 10444 0.9 7.2OE-02 AE000882.1 NT of the complete genome 1460 6587 -11760 0.8 7.2OE-02 AL163301.2 NT Homo saplens chromosome 21 segment HS21 Cl 01 21 segment HS21 Cl 01 1460 68-7 1761 0.8 7.2OE-02 AL1 63301.2 NT Homo sapiens chromosome virus type 1 lsolatei 26 reverse transcriptase (pol) gene, internal Human Immunodeficiency 2502 7599 0,9 7.2OE-02 U14794.1 NT fragment, partial ods 3810 8947 14043 0.8 7.2OE-02 AW298322.1 EST-HUMAN Ul-H-BWO-aji-a-05-0-Ul. sl NCI-C_Sub13 Homo sapiens cIDNA clone IMAGE2732049 3, 4997 6229 10100[151681 0.9 7.2OE-02 ABOO1562.1 NT Streptococcus mutans gene for glucose-l -phosphate urldylyltransferase, complete cds 1091 11380 1.3 7.1 OE-02 AB010711.1 NT Mus sp. mRNA for M1320, complete cds Human immunodeficiency virus type 1 (D9) proviral structural capsid protein (gag) gene, 1862 6980 12165 0.6 7.1OE-02 L02290.1 NT partial cds 517 5681 10802 1.2 7.OOE-02 Q07092 SWISSPROT COLLAGEN ALPHA I (XVI) CHAIN PRECURSOR 1484 6611 0.5 7.OOE-02 X96677.1 NT M.artiellia IVItcut-1 gene Homo sapiens cIDNA done IMAGE:509599 3' colon 172 12027 1.4 7.0012-02 AA056343.1 EST HUMAN (#937204) A66104.sl Stratagene 2994 8146 13247 1.7 7.OOE-02 AW138152.1 EST HUMAN Ul-H-Bil-a7-0-Ul.sl NC1- WAPJSuW Horno sapiens cIDNA done IMAGE:2716020 3' Homo saplens cDNA done 1375678 Ysimilar to gb:K03002 3822 8959 14055 1.0 7.ODE-02 AA815438.1 tST HUMAN a165a12.sl Soares_testis-NHT 60S RIBOSOMAL PROTEIN L32 (HUMAN); BT0407 Homo sapiens cDNA 12 7.OOE-02 BE070264.1 EST_HUMAN QV4-BT0407-280100-090-elO Page 41 of 196 Table 4

4047 9174 1.1 7.0012-02. AW792962.1 EST HUMAN CM0-1.1M0001-060300-270-e12 UM0001 Homo sapiens cDNA 4121 9247 14331 1.3 7.OOE-02 AF077821.1 NT Canis familiads inducible nitric oxide synthase mRNA, complete cds 503 5667 10788 1.8 6.9OE-02 AL.163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 503 5667 10789 1.8 6.9OE-02 AL163210.2 NT Horno sapiens chromosome 21 segment HS21C010 1311 6439 1.3 6.9OE-02 4507968 NT Horno sapiens regulator of Gz-selective protein signaling (ZGAP1) mRNA, and translated products 3724 8861 13953 1.4 6.9OE-02 006364 SWISSPROT 26S PROTEASOME REGULATORY SUBUNIT S3 (NUCLEAR ANTIGEN 21 D7) 3724 8861 13954 1.4 6.9OE-02 006364 SWISSPROT 26S PROTEASOME REGULATORY SUBUNIT S3 (NUCLEAR ANTIGEN 21D7) 5100 10196 1.1 6.9OE-02 AF079906.1 NT Rabies virus Isolate b615 glycoprotein gene, partial cds 1839 6 1 12142 1.1 6.8OE-02 AA496759.1 EST-HUMAN ae30f02.rl Gessler Wilms tumor Homo sapiens cIDNA clone IMAGE:897339 5'similar to 958 gb:M22382 MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (HUMAN); 1839 6958 12143 1.1 6.8OE-02 AA496759.1 EST HUMAN ae30f02.rl Gessler Wilms tumor Homo saplens cIDNA. clone IMAGE:897339 5'similar to gb:M22382 MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (HUMAN); 1865 6983 12167 1,4 6.8OE-02 AF156673.1 NT Homo sapiens putative hepatic transcription factor (WI3SCR14) gene, complete cds 3062 8213 13312 1.2 6.8OE-02 AA781996.1 EST-HUMAN ai75a06.sl Soares testis NHT Horno sapiens cDNA clone 1376626 3, 3062 8213 13313 1.2 6.8OE-02 AA781996.1 EST HUMAN a175a06.sl Soares testis_NI-IT Homo sapiens cIDNA, clone 1376626 3 3062 8213 13314 1.2 6.8OE-02 AA781996.1 EST HUMAN W75a06.sl Soares testis NHT Homo sapiens cDNA clone 1376626 3, 4453 9573 0.9 6.8OE-02 BE141076.1 EST HUMAN MRO-HT0069-07109"01-c05 HT0069 Homo sapiens cDNA 4773 9883 14976 0.9 6.8OE-02 M96150.1 NT Caenorhabditis elegans LDL receptor-related protein (Irp-1) gene, complete cds 1511 6638 0.8 6.7OE-02 AF1 15536.1 NT Oncorhynchus mykiss TAP1 protein (OnmyTAP1) mRNA, OrimyTAP101 allele, complete cds 1851 6969 12154 0.9 6.7OE-02 A1220285.1 EST HUMAN g979e04.xl Soares NFL T GBC S1 Homo saplens cDNA clone IMAGE:1841406 3' 2962 8113 13219 1.1 6.7OE-02 M13158.1 NT Yeast (S.pombe) cdc25+ gene (mitosis initiation), complete cds 2962 8113 13220 1.1 6.7OE-02 M13158.1 NT Yeast (S.pombe) cdc25+ gene (mitosis Initiation), complete cds 3316 8461 13573 0.8 6.7OE-02 AW301973.1 EST HUMAN xr85h011.xl INCLWAP- Lu26 Homo sapiens cDNA done IMAGE:2766961 3'similar to contains element TAR1 repetitive element; 3656 8795 13888 3.5 6.7OE-02 P17278 SWISSPROT HOMEOBOX PROTEIN HOX-D4 (CHOX-A) 1326 6455 11621 1.1 6.6OE-02 A1735509.1 EST HUMAN at12e09.xl Barstead aorta HPLIR136 Horno sapiens cDNA done IMAGE:2354920 3' similar to SW1W NYWO P08548 LINE-1 REVERSE TRANSCRIPTASE HOMOLOG.; 1347 6475 11641 0.5 6.6OE-02 AF245116.1 NT Drosophila melanogaster cactin mRNA, complete cds 3133 8282 1.3 6.6OE-02 Q13585 SWISSPROT MELATONIN-RELATED RECEPTOR (H9) 3418 8560 13671 8.6 6.6OE-02 R64306.1 EST HUMAN yi18b10.sl Soares placenta Nb2HP Homo saplens cONA done IMAGE:139579 3, 3432 8574 13686 2.2 6.6OE-02 7108357 NT Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA 3432 8574 13687 2,2 6.6OE-02 7108357 NT Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA 4904 10011 15090 8.4 6.6OE-02 Q61703 SWISSPROT INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR (IT1 HEAVY CHAIN H2) 4904 10011 15091 8.4 6.6OE-02 061703 SWISSPROT INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR (ITI HEAVY CHAIN H2) 5167 1 F2 6-1 15332 1.3 6.60E-02 AF204882.1 NT Amsacta albishiga nudeopolyhedrovirus AcORF17 homolog gene, complete cds 971 6117 11275 0.6 6.5OE-02. 7706068 NT Homo saplens E2F-like protein (LOC51270), mRNA 6497 11663 1.5 6.5OE-02 U47624.1 _. NT Xenopus laevis alpha(E)-catenin mRNA, complete cds Page 42 of 196 Table 4

1702 6825 12006 0.8 6.5OE-02 AE000764.1 NT Aquifex aeolicus section 96 of 109 of the complete genome 561 5723 10839 0.5 6.4OE-02 X94549.1 NT A.carterae precursor of peddinin- chlorophylia-protein (PCP) gene 4802 8133 13240 1.4 6.40E-02 6996923 NT Mus musculus histone deacetylase 5 (Hdac5), ffiRNA 1 5180 10272 8.8 6.4OE-02 6996923 NT Mus musculus histone deacetylase 5 (Hdac5), mRNA 1720 6843 12022 0.9 6.30E-02 AF109905.1 NT Mus musculus major histocompatibility locus class Ill regions Hsc70t gene, partial cds; smRNP, G7A, NG23, MutS homolog, CI-CP, NG24, NG25, and NG26 genes, complete ods; and unknown genes 3552 8692 2.1 6.30E-02 P37092 SWISSPROT HEAT SHOCK PROTEIN 70 HOMOLOG.

4157 9283 14363 3.4 6.20E-02 AL161572.2 NT Arabidopsis ih-aiiana DNA chromosome 4, condg fragment No. 68 4243 9368 1.1 6.2OE-02 AF271235.1 NT Rattus norvegicus differentation- associated Na-dependent Inorganic phosphate cotransporter (DNPI) mRNA, complete cds 4347 9470 14559 0.8 6.2OE-02 AW951243.1 EST-HUMAN EST363313 MAGE resequences, MAGA Homo sapiens cDNA 4347 9470 14560 0.8 6.2OE-02 AW951243.1 EST HUMAN EST363313 MAGE resequences, MAGA Homo sapiens cDNA 4479 9599 5.4 6.20E-02 Q62191 SWISSPROT 52 KI) RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (RO(SS-A))l (R052) 256 5444 10570 1,5 6.1 OE-02 D16471.1 NT Human mRNA, Xq terminal pordon 3909 2.7 6.1OE-02 U73325.1 NT Arabidopsis thallana K+ inward recbfng channel protein (AtKCl gene, complete cds 5150 10245 15317 1.0 6.1 OE-02 AB040897,1 NT Homo sapiens mRNA for KIAA1464 protein, partial ods 1241 6370 11529 1.4 6.OOE-02 AE001777.11 NT Thermotoga maddma section 89 of 136 of the complete genome 2547 7643 1.5 6.OOE-02 AW8161 19.1 EST HUMAN MR3-ST0220-120100-025-d04 ST0220 Homo sapiens cDNA 2641 7730 12931 0,6 6.OOE-02 AW968848.1 EST HUMAN EST380924 MAGE resequences, MAW Homo sapiens cDNA 2745 7829 0.6 6.OOE-02 A13031289.1 NT Mesocestoldes cord mitochonddal DNA, NADH dehydrogenase subunit 4, tRNA-Gin, IRNA Phe, tRNA-Met, ATPase subunit 6, and NADH deWydrogenase subunit 2 2906 5298 10424 1.2 6.OOE-02 AA188730.1 EST HUMAN zp78c04xl Stratagene HeLa cell s21 937216 Homo sapiens cDNA done IMAGE:626310 S' 2906.5298 10425 1,2 6.OOE-02 AA188730.1 EST-HUMAN zp78c04xl Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone IMAGE:626310 5 3191 8340 13453 1.2 6.OOE-02 AA372376.1 EST HUMAN EST84266 Colon adenocardnoma IV Horno sapiens cDNA Vend similar to tissue-specific protein 3191 8340 13454 1.2 6.OOE-02 AA372376.1 EST-HUMAN EST84266 Colon adenocardnoma IV Homo sapiens cDNA 5' end similar to Ussue-specific protein 4893 10000 15083 1.2 6.OOE-02 Z67739.2 NT Streptococcus pneumoniae parC, parE and transposase genes and ORF DNA 229. 5420 10543 1.2 5.90E-02 AW934719.1 EST-HUMAN RC1 -DT0001 -290100-012- el 0 DT0001 Homo sapiens cDNA 2955 8106 13213 2.6 5.90E-02 AF190269.1 NT Mus musculus p53 tumor suppressor gene, exon 10 and 11, partial cds; alternatively spliced 4517 9636 1.1 5.9OE-02 4506466 NT Homo sapiens radiyJn (RDX) mRNA 4770 9881 14975 0.9 5.9OE-02 AF006304.1 NT Saccharomyces cerevislac protein Ine phosphatase (PTP3) gene, complete cds g917 6063 1.5 5.8OE-02 D90110.1 NT Thlobacillus ferrowddans merC, merA genes and URF-1 0 7981 1.1 5.8OE-02 AJ223621.1 NT Populus trichocarpa CCoAOW1 gene, exon 1 to exon 5 2830 31 8752 13846 1.4 5.8OE-02 AE001775.1 NT a madtima section 87 of 136 of the complete genome 6131 42 1,9382 14463 4.4 5.8OE-02 AW051927.1 EST HUMAN wx24c02.xl NCI CGAP Kidl 1 Horno saplens cDNA done IMAGE:2544578 3' Page 43 of 196 Table 4

In 4257 9382 14464 4.4 5.8OE-02 AW051927.1 EST-HUMAN wx24c02.xl NCI WAP_Kidl 1 Horno sapiens eDNA done IMAGE:2544578 3' 4447 9567 14664 4.2 5.81OE-02 A1247505.1 ESTHUMAN qh56f01.xl Soares-fetal- liver - spleen - 1 INIFI-S_SI Homo sapiens cDNA done IMAGE:1848697 Ysimilar to gb:M1342 COAGULATION FACTOR XI PRECURSOR (HUMAN); 4447 9567 14665 4.2 5.8OE-02 A1247505.1 EST HUMAN qh56f011.xl Soares- fetal-liveLspleen - INFILS - S1 Horno sapiens cIDNA done IMAGE:1848697 3'similar to gb:M1342 COAGULATION FACTOR XI PRECURSOR (HUMAN); 4471 9591 2.0 5.8OE-02 AF096264.1 NT Gallus gallus tyrosine kinase JAK1 (JAK1) mRNA, complete ods 2659 7748 12946 1.0 5.7OE-02 A1708246.1 ESTHUMAN as24f06.xl Barstead aorta HPI-RI36 Homo sapiens cDNA clone IMAGE:2318147 Ysimilar to gb:X14850 cdsl HISTONE H2A.X (HUMAN); 2659 7748 12947 1.0 5.7012-02 A1708246.1 EST-HUMAN as2006.xl Barstead aorta HPI-RI36 Homo sapiens cDNA clone IMAGE:2318147 Ysimilar to gb:X14850 cdsl HISTONE H2A.X (HUMAN); 3018 8170 13270 1.3 5.70E-02 A1081644.1 EST-HUMAN ou63b05.sli NCI-C_W2 Horno sapiens cDNA done IMAGE: 1632465 Ysimilar to WP:C37A2.2 CE08611; 3033 8185 13286 1.3 5.7OE-02 AF1 19117.1 NT Homo sapiens dopamine transporter (SLC6A3) gene, complete cds 3731 8868 13960 1,8 5.70E-02 AW966791.1 EST-HUMAN EST378865 MAGE resequences, MAGI Homo sapiens cDNA 1510 6637 11806 0.9 5.6OE-02 AF094455.1 NT Hydrocotyle rotundifolia ribosomal protein L16 (rpil 6) gene, Intron; chloroplast gene for chloroplast product 4217 9341 14425 0,9 5.60E-02 A.1390487.1 NT Candida albicans mitochondrial mRNA for NADH dehydrogenase (ndel gene) 4540 9659 14747 1.3 5.60E-02 AB01 3100.1 NT Lycopersicon esculentum LE- ACS6 mRNA for 1 --aminocydopropane-l-carboxylate synthase, complete cels 4598 9712 14804 1.1 5.6OE-02 AA290599.1 EST-HUMAN zs45c01.sl NCI_PGAF_GCB1 Homo saplens cDNA done IMAGE:700416 3' 2618 7708 12907 3.0 5.50E-02 X97869.1 NT H.sapiens gene encoding La autoanfigen 3179 8328 13441 3.6 5.5OE-02 6755501 NT Mus musculus SH3 domain protein 1 B (Sh3dl B), mRNA 4777 9887 14980 0.8 5.5OE-02 AF161266.1 NT Murray Valley encephalitis virus strain MVE-1-51, complete genome 2986 8138 1.0 5.4OE-02 AJ277468.1 NT Oryza sativa rbb13-1 gene for putative Bowman Birk trypsin inhibitor 3375 8518 6.3 5.40E-02 BE073468.1 EST-HUMAN RCS-BT0559-140200-012-CO3 BT0559 Homo sapiens cDNA 4908 10015 15095 0.9 5.40E-02 U53528.1 NT Xenopus laevis homeobox protein (Vox-1) mRNA, complete cds 5108 10204 15283 1.0 5.4012-02 M96761.1 NT Mus musculus pprotein (mdda) gene, exons 1 and 2 1037 6177 11329 0,6 5.3OE-02 AW391248.1 EST-HUMAN QVO-ST0213-021299-062- a09 ST0213 Homo saplens cDNA 1037 6177 11330 0.6 5.3OE-02 AW391248.1 EST HUMAN QVO-ST0213-021299-062- a09 ST0213 Homo sapiens cDNA 1489 6616 11787 6.6 5.30E-02 T94759.1 EST HUMAN ye37f12.rl Stratagene lung (#937210) Homo sapiens cIDNA clone IMAGE:1 19951 5'similar to gb:K01 506 HLA CLASS 11 HISTOCOMPATIBILITY ANTIGEN, DP(1) ALPHA CHAIN (HUMAN); 2447 7546 12754 1.1 5.30E-02 A.1276408.1 NT Pseudomonas putida ttgS gene 2912 8063 13170 3.9 5.30E-02 M58417.1 NT Drosophila melanogaster laminin B2 gene, complete cels 2912 8063 13171 3.9 5.3OE-02 M58417.1 NT Drosophila melanogaster laminin B2 gene, complete cels 3113 8262 13366 4.6 5.30E-02 AJ276408.1 NT Pseudomonas putida ttgS gene 4506 9625 14717 1.2 5.3OE-02 AJ01 1048.1 NT Arabidopsis thallana e115 gene, exons 1 -11 5021 10122 15194 7.3 5.3OE-02 M80463.1 NT Mus musculus caudal type homeobox-1 (Cdx-1) gene, complete cds 7345 160.0 5.20E-02 5031908 NT Homo sapiens meprin A, alpha (PABA peptide hydrolase) (MEP1A) mRNA 1 Page 44 of 196 n m Table 4

4181 9307 14386 3.4 5.2OE-02 U07132.1 NT Human steroid hormone receptor Ner-i mRNA, complete cds 4490 9610 14703 2,4 5.20E-02 X58743.1 NT H.sapiens HCK gene for tyrosine kinase (PTK), exon 12 4490 9610 14704 2.4 5.2OE-02 X58743.1 NT H.sapiens HCK gene for tyrosine kinase (PTK), exon 12 4633 9747 14843 1.0 5.2OE-02 L33246.1 NT Drosophila melanogaster lament protein homolog (sepl) gene, complete eds 2313 7416 1.0 5.1OE-02AL134071.1 EST-HUMAN DI(FZp547D073-rl 5.47 (synonym: hfbrl) Horno sapiens cDNA clone DKFZp5471)073 & 3610 8749 13843 3.6 5.11OE-02 AF094269.1 NT HIV-1 isolate 2001-4-37 from USA, envelope glycoproteln (env) gene. partial ods 3644 8783 5.8 5.1OE-02 AA652267.1 EST HUMAN ns69d0I.sl NCI-CGAP_Pr2 Homo sapiens cDNA done IMAGE: 1188865 similar to contains Alu repetitive elementcontains element PTR5 repetitive element; 474 5640 10766 0.7 5.OOE-02 AF098004.1 NT Mus musculus fatty acid amide hydrolase 9!ne, exon 10 1186 6318 11474 2.4 5.OOE-02 Z99104.1 NT Bacillus subtills complete genome (section 1 of 2l): from 1 to 213080 1947 7064 12258 1.5 5.OOE-02 P02810 SWISSPROT SALIVARY ACIDIC PROLINE- RICH PHOSPHOPROTEIN 112 PRECURSOR (PRP-1/PRP-3 (PRP-21PRP-4) (PJF-FIPIF-S) (PROTEIN A/PROTEIN C) [CONTAINS: PEPTIDE P-C] 2788 6116 11274 0.6 5.OOE-02 U72742.1 NT Oryctolagus cuniculus UDP- glucuronosyitransferase (1)GT2B13) mRNA, complete cds 3295 8441 1.2 5.OOE-02 7305610 NT Mus musculus Unc-51 like kinase 2 (C. clegans) (U1k2), mRNA 3629 8768 13862 5.5 5.OOE-02 U12769.2 NT Antheraca pemyi period clock protein homolog mRNA, complete cds 4890 9997 15080 0.9 5.OOE-02 AF188530.1 NT Homo sapiens ubiquitous tetratricopepfide containing protein RoXaN mRNA, partial cds 218 5410 9.9 4.9OE-02 M14230.1 NT Chicken 28-kDa vitamin D-dependent calcium-binding protein (Cal3P-28) mRNA, complete cds 413 5581 1.9 4.9OE-02 D26607.1 NT Homo sapiens endothelial nitric oxide synthase gene, complete cds 3251 8398 13504 1.9 4.9OE-02 P54258 SWISSPROT ATROPHIN-1 (DENTATORUBRALPALLIDOLUYSIAN ATROPHY PROTEIN) 3522 8663 0.9 4.9012-02 AA1 88940.1 EST-HUMAN zq48a12.sli Stratagene hNT neuron (#937233) Homo sapiens cONA clone IMAGE:632926 3' similar to contains Alu repetitive element;contalns element MSR1 repetitive element:

3543 8684 13784 1.2 4.9OE-02 AA400914.1 EST HUMAN W8a03.sl Soares tesds_NHT Homo sapiens cDNA done IMAGE:728428 3 3543 8684 13785 1.2 4.9012-02 AA400914.1 EST HUMAN zt78a03.sl Soares_testis_NHT Homo sapiens cDNA done IMAGE:728428 3' 4733 9844 14936 1.9 4.9OE-02 AW167821.1. EST-HUMAN xg56g110.xl NCI CGAP W4 Homo sapiens cDNA done IMAGE:2632386 3' 4733 9844 14937 1.9 4.9OE-02 AW167821.1 EST HUMAN x956g10.xl NCI CGAP U14 Homo sapiens eDNA done IMAGE:2632386 X 327 5509 10636 1.5 4.8OE-02 D16471.1 NT Human mRNA, Xq terminal portion 328 5509 10636 0.7 4.8OE-02 D16471.1 NT Human mRNA, Xq terminal portion 480 5646 10771 2.6 4.8OE-02 AF0031 00.1 NT Arabldopsis thallana AP2 domain containing protein RAP2.7 mRNA, partial cds 2227 7334 12546 0.7 4.8OE-02 W51983.1 EST HUMAN zc49b02.si Soares senescenLflbroblasts - NbHSF Horno sapiens cDNA done IMAGE:325611 YjImilar to gb:M30938 LUPUS KU AUTOANTIGEN PROTEIN P86 (HUMAN); 3172 8321 13435 2.1 4.8OE-02 X17144.1 NT Tetrahymena rostrata histone H311 and histone H411 Intergenic DNA 4572 9690 1.7 4.8DE-02 Z54280.1 NT S.scrofa gene for skeletal muscle ryanodine receptor 1 5076 10174 15254 1.3 4.8OE-02 U91914.1 NT Streptoooccus constellatus Dalanine:D-alanine. ligase gene, partial cds 4940 10046 15118 0.8 4.7OE-02 6981261 NT Rattus norvegicus Nesfin (Nes), mRNA 722 11016 0.9 4.6OE-02 AE000445.1 NT Escherichia col) K-12 MG1655 section 335 of 400 of the complete genome 6399 1.1 4.6OE-02 A1014255.1 EST HUMAN am50d02.sli Johnston frontal cortex Homo sapiens cDNA clone IMAGE:1538979 3'similar to TR:1390533 P9053 LIMA;contains element LTRI repetitive element Page 45 of 196 Table 4

2438 7537 12744 0.9 4.6OE-02 AW236023.1 EST-M-MAN xn24f03.xl NCJ_CGAF_Kidl 1 Homo sapiens cIDNA clone IMAGE:2694653 Ysimilar to MGRF1 HUMAN Q12849 G-RICH SEQUENCE FACTOR-I; 2777 5457 10584 0.5 4.6OE-02 BE153583.1 EST-HUMAN PMO-HT0339-251199-003- g05 HT0339 Homo sapiens cDNA 2977 8128 13235 1.0 4.6OE-02 BE153583T- EST-HUMAN PMO-HT0339-251 1 9M03- g05 HT0339 Horno sapiens cDNA 3451 8128 13235 1.0 4ME-02 BE153583.1 EST HUMAN PMO-HT0339-251199-003-g05 HT0339 Homo sapiens cDNA 4033 9161 1.2 4.6OE-02 AF220365.1 NT Mus musculus nudeolar RNA helicase It/Gu (ddx21) gene, complete cds 439 5605 10739 0.6 4.509-0-2 P22448 SWISSPROT RETINOIC ACID RECEFT-O-R BETA (RAR-BETA) 1200 6332 11487 0.9 4.5OE-02 AF005730.1 NT Marburg virus strain WS. Afdca/Johannesburg/1975/Ozolin VP35 gene, complete eds 1200 6332 11488 0.9 4,5OE-02 AF005730.1 NT Marburg virus strain WS. Afdca/Johannesburg/1975/Ozolin VP35 gene, complete cds 1763 6884 12067 1,3 4.5OE-02 P32182 SWISSPROT HEPATOCYTE NUCLEAR FACTOR 3- BETA (HNF-30) 2063 7175 12382 0.7 4.5OE-02 AE003964.1 NT Xylelia fastidlosa, section 110 of 229 of the complete genome 3662 8801 13896 3.8 4.5OE-02 A1.1 63278.2 NT Homo sapiens chromosome 21 segment HS21C078 1008 6148 11302 1.3 4.4OE-02 L19295.1 NT Drosophila melanogaster extradenticle (EXD) mRNA, complete cds 2049 7162 2.2 4.4OE-02 P31568 SWISSPROT HYPOTHETICAL PROTEIN (ORF 2280) 2440 7539 12746 1.3 4.4OE-02 AW875475.1 EST-HUMAN QV2-PT0012-010300-070- 902 PT0012 Homo sapiens cDNA 3303 8448 13558 1.0 4.4OE-02 M64798.1 NT Methanobacterlum formicicurn fdhC gene, complete cds 3588 8727 13823 1.9 4.4OE-02 AF159160.1 NT Myxococcus xanthus sednelthreonine kinase PknIO (pkn10) gene. complete cds 4527 9646 14735 1.1 4.4OE-02 AF109907.1 NT Homo sapiens S164 gene, partial cds; PS1 and hypothetical protein genes, complete ods; and S 171 gene, partial cds 4527 9646 14736 1.1 4.4OE-02 AF109907.1 NT Homo sapiens S164 gene, partial cds; PS1 and hypothed"i protein genes, complete cds:

and S171 gene, partial cds 4637 9751 3,1 4.4OE-02 AJ222689.1 NT Ovis aries CCAAT-enhancer binding protein epsilon gene 1690 6814 1.2 4.3OE-02 T6791 1.1 EST HUMAN yc28a09.sl Stratagene liver (#937224) Homo sapiens cDNA done IMAGE:81976 3' similar to contains Alu repetitive element; 3383 8526 13633 7.0 4.3OE-02 AL1 63210.2 NT Homo sapiens chromosome 21 t,, ;.nent HS21 C01 0 3609 8748 1.1 4.3OE-02 AF060568.1 NT Homo sapiens promyelocytic leukernia dric finger protein (PLZF) gene, complete cds 4334 9457 14545 3.5 4.3OE-02 F17181.2 EST-HUMAN HSPD01704 HM1 Homo sapiens cDNA done NOTAVAIL01704 5141 10236 15311 0.9 4.3OE-02 X51594.1 NT Pea P4 organ specific gene 878 6026 11186 0.8 4.2OE-02 AW003645.1 EST-HUMAN wx34901.xl NC1-WAP_Pitl Homo saplens cDNA clone IMAGE:2545584 Ysimilar to TR:Q63291 Q63291 1.1 RETROPOSON, ORF2 IVIRNA;contains Ll.t3 1.1 L1 repetitive element; 3117 8266 13370 0.9 4.2012-02 A1493472.1 EST HUMAN qy95f10.xl NCI COAP_Brn25 Homo sapiens cDNA done IMAGE:2019787 Ysimilar to gb:M35718 FIBOBLAST GROWTH FACTOR RECEPTOR I3FR-2 PRECURSOR (HUMAN); 5172 10266 1.1 4.2OE-02 D63484.1 NT Human mRNA for KIAA0150 gene, partial ods 2643 7732 12933 1.0 4.1 OE-02 AE002330.2 NT Chlarnydia muridarum, sic- tion 60 of 85 of the complete genome 3824 8961 14058 0.9 4.1OE-02 BE297236.1 EST-HUMAN 601177907F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE:3533353 S' 3824 5961 14059 0.9 4.1 OE-02 BE297236.1 EST-HUMAN 601177907F1 NIH MGr17 Homo sapiens ciDNA done IMAGE:3533353 5' 4372 9494 7.4 4.1OE-02 AW893484.1 EST HUMAN OV1-NNO012-180400-164-f06 NNO012 Homo saplens cDNA 2816 7967 13071 1.4 4.OOE-02 U18867.2 NT Mus musculus uropffhydnogen Ill synthase mRNA, complete cds 3206 8354 13464 2.7 4.OOE-02 AB040904.1 NT Horno sapiens mRNA for KIAA1471 protein, partial ods 1 y- ') 6451 11616 1.3 3.9OE-02 P41047 SWISSPROT FAS ANTIGEN LIGAND Page 46 of 196 Table 4

1918 7035 12223 1.1 3.9OE-02 AJ403386.1 NT M.musculus DNA for desminbinding fragment DesD7 2671 7758 0.8 3.9OE-02 4506862 NT Homo sapiens succinate dehydrogenase complex, subunit C, Integral membrane protein, 15k1D (SIDHC) mRNA 5105 10201 15280 1.0 3.9OE-02 8924019 NT Homosapiens hypothetical protein PRO1163 (PRO1163), RNA 5105 10201 15281 1.0 3.9OE-02 8924019 NT Homo sapiens hypothetical protein PROI 163 (PRO1 163), nRNA 1568 6694 1.3 3.8OE-02 AJ229042.1 NT Horno, saplens 959 kb config between AMIL1 and CBR1 on chromosome 21q22, segment 213 976 6121 11278 1.6 3.7OE-02 P19137 SWISSPROT LAMININ ALPHA-1 CHAIN PRECURSOR (LAMININ A CHAIN) 1366 6494 11660 0.9 3.7OE-02 1-14561.1 NT Homo sapiens plasma membrane calclum ATPase isoform 1 (ATP2B1) gene, alternative splice PLcucts, partial cds 2190 7299 12514 2.3 3.7OE-02 A1984806.1 EST-HUMAN wr85e08.xl NCI_PGAP KM1 1 Horno, saplens cDNA done IMAGE:2494502 3 2533 7629 12831 0.9 3.7012-02 AB018261.1 NT Homo sapiens mRNA for KIAA0718 protein, partial cds 3012 8164 13265 0.8 3.7OE-02 P79944 SWISSPROT EOMESODERMIN 3408 8550 1.1 3.7OE-02 6680541 NT Mus musculus potassium large conductance pH-sensifive channel, subfamily M, alpha member 3 (Kcnma3), mRNA 3604 8743 13838 0.9 3.6OE-02 X73221.1 NT H.vulgare SsI gene for sucrose synthase 3612 8751 13845 0.8 3.6OE-02 AL096806.1 NT Horno, sapiens genomic region containing hypervarlable minisatellites chromosome 1 Ofl 0q26.3] of Homo sapiens 5135 10230 15305 0.8 3.6OE-02 AL096810.1 NT Homo sapiens genomic region containing hypervarlable minisatellites chromosome 0q26.31 of Homo sapiens 877 6025 11185 1.2 3.5012-02 U09506.11 NT Drosophila melanogaster tiggrin mRNA, comelete cds 992 6135 11290 0.5 3.5OE-02 AF253417.1 NT Homo saplens microsornal epoAde hydrolase (EPHX1) gene, complete cds 1188 6320 3.5 3.5OE-02 AW772365.1 EST HUMAN hn72c08.xl NCI CGAP KM1 1 Homo sapiens cDNA done IMAGE:3033422 3 3271 8416 1.5 3.5OE-02 T29124.1 EST HUMAN EST69951 Human Whi tood cells Homo sapJens cIDNA Vend similar to T-cell receptor, alpha chain, V region (HT:3229) 3663 8802 13897 0.9 3.5OE-02 X61 109.1 NT G.godlia beta-globin gene 4120 9246 14330 2.0 3.5OE-02 AE001773.1 NT Thermotoga marifirna section 85 of 136 of the complete genome 4220 9344 14427 1.4 3.5OE-02 P53780 SWISSPROT CYSTATHIONINE BETA-LYASE PRECURSOR (CBL) (BETA-CYSTATHIONASE) (CYSTEINE LYASE).

4536 9655 1.0 3.5OE-02 AW958469.11 EST-HUMAN EST370539 MAGE resequences, MAGE Homo sapiens eDNA 5154 10249 0.8 3.5OE-02 P47144 SWISSPROT HYPOTHETICAL 80.7 KD PROTEIN IN SOD1-CPA2 INTERGENIC REGION 1035 6175 11327 1,3 3.4OE-02 AW274020.1 EST HUMAN xv26d07.xl Soares-NFL-T- GBC,.pl Homo sapiens cDNA done IMAGE:2814253 Ysimilar to SW:C21 1-HUMAN P53801 PUTATIVE SURFACE GLYCOPROTEIN C21 ORF1 PRECURSOR; 1817 6936 12126 0.6 3.4012-02 AA398742.1 EST-HUMAN zt75f08.sl Soares testis NHT Homo sapiens cIDNA done IMAGE:728199 3' 2343 7445 12652 0.8 3.4OE-02 T57160.1 EST-HUMAN yc20e06xl Stratagene. lung (#937210) Homo sapiens cDNA done IMAGE:81250 5' similar to contains MER29 repeflUv6 element 3384 8527 13634 1.3 3.4OE-02 AL163208.2 NT Homo sapiens chromosome 21 segment HS21 C008 3845 8981 14082 3.2 3.4OE-02 AW794952.1 EST-HUMAN RC6-UM0015-219200-021- AlO UM0015 Homo saplens cIDNA 449.4 9614 14706 2.4 3.4OE-02 X59799.1 NT HOMOLOAG) M.musculus S-antigen gene promoter region L__4 - 10095 3.4 3.4OE-02 Q264 7 SWISSPROT LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN Page 47 of 196 Table 4

5010 10112 15182 1.3 3.4OE-02 AJ012469.1 NT Caenorhabdids elegans mRNA for DY9-1 protein, partial 368 5546 4,5 3.3OE-02 AA398735.1 EST HUMAN zt75e08.sl Soares_testis NHT Homo sapiens cDNA done IMAGE:728198 3' 1151 6286 11439 4.5 3.3OE-02 A13035867.1 NT Cricetulus griseus CYP2A1 7 mRNA for cytochrome P450 2A1 7, complete cds 1621 6747 11915 1.1 3.3OE-02 AF1 10763.1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene, complete cds 1705 6828 1,3 3.3OE-02 AE000700.1 NT' Aquifex aeolicus section 32 of 109 of the complete genome 2038 7151 1.0 3.3OE-02 R091 12.1 EST-HUMAN A25c09xl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone 1 MAGE: 127888 5 2448 797- 12755 1.1 3.3OE-02 T02847.1 EST-HUMAN FB12A2 Fetal brain, Stratagene Homo saplens cDNA done FB12A2 Yend 2448 7547 12756 1.1 3.3OE-02 T02847.1 EST HUMAN FB12A2 Fetal brain, Stratagene Horno sapiens cDNA clone FB12A2 Yend 4086 6747 11915 2.2 3.3OE-02 AF1 10763,1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHLI) gene, complete cds 4366 9488 14579 1.9 3.3OE-02 6755862 NT Mus musculus tumor rejection antigen gp96 (Tral), mRNA 127 5323. 10455 1.1 3.2OE-02 A.1002005.1 NT Oryetola us cuniculus gene encoding lleal sodium-dependent bile acid transporter 1110 6247 11399 4.3 3.2OE-02 AF096275.1 NT Drosophila melanogaster heat shock protein 68 (hsp68) gene, hsp68d aliele, complete cds 1110 6247 11400 4.3 3.2OE-02 AF096275.1 NT Drosophila melanogaster heat shock protein 68 (hsp68) gene, hsp68d allele, complete cds 1734 6857 12035 1.1 3.2OE-02 AF128894.1 NT Homo sapiens telomerase reverse transcriptase (TERT) gene, exons 7-16 and complete cds 2072 7184 0.9 3.2OE-02 P28955 SWISSPROT LARGE TEGUMENT PROTEIN 2809 5323 10455 1.2 3.2OE-02 AJ002005.1 NT Oryctolagus cuniculus ge e encoding ileal sodium-dependent bile acid transporter 3653 8792 13886 1.1 3.2OE-02 AL1 63203.2 NT Homo sapiens chromosome 21 segment HS21 C003 4124 9250 12.4 3.2012-02 X94768.1 NT H.sapiens RP3 gene (Xl-RP gene 3) 4658 9770 14865 2,9 3.2OE-02 AF1 14182.1 NT SaAfraga nidifica maturase (matK) gene, chloroplast gene encoding chloroplast protein, partial cds 1239 6368 0.7 3.1 O-E-02 4503416 NT Homo sapiens dual specificity phosphatase 4 (DUSP4) mRNA 1283 6413 11575 1.3 3.1 OE-02 P18845 SWISSPROT NEURONAL ACETYLCHOLINE RECEPTOR PROTEIN, ALPHA-3 CHAIN PRECURSOR (G17-ALPHA-3) 1501 6627 1.0 3.1 OE-02 AF200465.1 NT Homo sapiens coxsackievirus and adenovirus receptor (CXADR) gene, complete cds; and ANA gene, partial cds 1850 6968 12153 1.4 3.1 OE-02 6671564 NT Mus musculus adaptor-related protein complex AP-3, delta subunit (Ap3d), mRNA 1931 7048 1.1 3.1 OE-02 Z50097.1 NT Drosophila melahogaster mRNA for headcase protein 4767 9878 14972 1.0 3.1OE-02 AW835313.1 EST-HUMAN QVO-LT0014-250200-129- h09 LT0014 Homo sapiens cDNA 1606 6733 1.0 3.OOE-02 AF187125.1 NT Pityoktelnes minutus cytochrome oxidase 1 gene, partial cds; mitochondrial gene for mitochondrial product 2541 7637 12837 1.1 3.OOE-02 AA402242.1 EST HUMAN zt65h03xl SoaT_Mds_NHT Homo sapiens cDNA clone IMAGE:727253 5' 3518 8659 13766 1.1 3.0012-02 M94176.1 NT Saccharomyces cerevislae stem- loop mutation supressor SSI-2 gene, complete cds 3685 8823 1.0 3.OOE-02 AW820223.1 EST-HUMAN OV2-ST0296-150200-040-e09 ST0296 Homo sapiens cDNA 3866 9002 1.2 3.OOE-02 AA364003.1 EST HUMAN EST74530 Pineal gland 11 Homo sapiens cDNA Vend 2385 7942 12689 1.1 2.9012-02 AF228703.1 NT Homo sapiens mitochonddal glutathione reductase and cytosolic glutathione reductase 2 7548 12757 0.5 2.90E.02 4502272 NT (GRD1) gene, complete ods, alternatively sticed falpha 3 polypeptl e (ATP1A3), mRNA Homo sapiens ATPase, Na+M transporting, d Page 48 of 196 Table 4

3851 8987 14087 0,9 2.9OE-02 H72805.1 EST HUMAN yuO7e1O.rl Soares fetal liver spleen 1I NFLS Homo saplens cIDNA done IMAGE:233130.5' 4950 10055 15125 1.3 2.9OE-02 X65137.1 NT Smulgare pepC gene for PEP carboxylase 4950 10055 15126 1.3 2.9OE-02 X65137.1 NT S.vulgare pepC gene for PEP carboxylase 553 5716 0.9 2.8OE-02 AW970153.1 EST HUMAN EST382234 MAGE resequences, MAGK Homo sapiens cIDNA 3321 8465 13578 1.3 2.8OE-02 AF066063.1 NT Homo sapiens refinal fascin (FSCN2) gene, exon 2 3321 8465 13579 1.3 2.8012-02 AF066063.1 NT Horno sapiens retinal fascin (FSCN2) gene, exon 2 4214 9338 1.0 2.8OE-02 8393751 NT Rattus norvegicus microtubule- assoclated protein tau (Mapt), mRNA 1473 6600 11774 1.3 2,70E-02 U66059.1 NT Human germline T-cell receptor beta chain Dopamine-beta-hydroxylase-like, TRY1, TRY2, TRY3, TCRI3V27S1P. TCIRW22S1A2N1T, TCR13V9S1AIT, TCRI3V7S1A1N2T, TCRI3V5S1A1T, TCRI3V13S3, TCRI3V6S7P, TCR13V7S3A2T, TCIRW13S2A1T.

TCIRW9S2A2PT, TCRBV7S2A1 N4T, TCRI3V1 3S911 3S> 3385 8528 13635 2.0 2.7012-02 AL161494.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 6 - 9233 14315 2.1 2.7OE-02 N47258.1 EST-HUMAN yy86h12xl Soare!_mulfiple- scJerosis-2NbHMSP Homo sapiens cIDNA done IMAGE:280487 4107 5' 4107 9233 14316 2.1 2.7OE-02 N47258.1 EST-HUMAN yy86h12xl Soare_mulfiple- scierosis_2NbHMSP Homo, sapiens cDNA done IMAGE:280487 5' 5194 10285 15352 1.0 2.7OE-02 A1825422 1 EST HUMAN w1J18e04.xl NCI CGAP GC6 Horno saplens DNA done IMAGE:2306046 3' 5194 10285 15353 1.0 2.7OE-02 A1825422.1 EST-HUMAN wb18e04.xl NCI CGAP GC6 Homo saplens dDNA done IMAGE:2306046 3' 559 5721 10837 1.1 2.6OE-02 AIL163282.2 NT Homo sapiens chromosome 21 segment HS21C082 1345 6473 1.0 2.6OE-02 AW850515.1 EST-HUMAN IL3-CTO219-280100-062-CO9 CT0219 Homo sapiens cDNA 2315 7418 12625 1.1 2.6OE-02 AA490021.1 EST HUMAN ab02b02.s1 Stratagene fetal retina 937202 Homo sapiens cDNA done IMAGE:839595 3' 2317 7420 12627 1.0 2.6OE-02 6754241 NT Mus musculus hishdine rich calcium binding protein (Hrc), mRNA 2317 7420 12628 1.0 2.6OE-02 6754241 NT Mus musculus hisfidine rich calcium binding protein (Hre), mRNA 2885 8036 1.6 2.6OE-02 AF109906.1 NT Mus musculus MI-IC class lit region RD gene, partial cds; Bf, C2, G9A, NG22, G9, HSP70.

HSP70, I-ISC70t, and smRNP genes, complete cds, G7A gene, partial cds. and unknown genes 3285 8430 13538 1,0 2.6OE-02 AF184597.1 NT Citrus unshij ADP-glucose pyrophosphorylase small subunit (agpS) mRNA, complete cds 3883 9019 11 2.6OE-02 AW181945.1 EST HUMAN xj68f09.xl Soares NFL,_GBC S1 Homo sapiens cDNA done IMAGE:2662409 3' 4811 9921 15013 3.7 2.6OE-02 L12032.1 NT Chicken dorsalin-l mRNA, complete cds 4998 10101 15169 1.7 2.6OE-02 AE002014.1 NT Deinococcus radiodurans R1 section 151 of 229 of the complete chromosome 1 5025 1012 2.1 2.6OE-02 AW241154.1 EST HUMAN xa52b04.xl NCI - CGAP-Sa.r4 Homo sapiens cIDNA clone IMAGE:2570383 Ysimilar to SW:Y069-HUMAN 015041 HYPOTHETICAL PROTEIN K1AA0069; 521 5685 10806 1.5 2.5OE-02 A1793130.1 EST HUMAN on26f06.y5 NCI CGAP Lu5 Homo sapiens cDNA clone IMAGE:1557827 5' 521 5685 10807 1,5 2.5OE-02 A1793130.1 EST HUMAN on26f06.yS NCI_PGAP_Lu5 Homo sapiens cDNA done 1A4AGE..1557827 5' 1992 7108 12310 1.6 2.5OE-02 6680967 NT Mus musculus procollagen, type IV, alpha 3 (Col4a3), mIRNA 2735 7819 0.9 2.5OE-02 U12571.1 NT Rattus no qicus rabphilin-3A mRNA, complete cds 2926 8077 13184 4,4 2.5OE-02 X99697.1 NT H.carterae mRNA for fucoxanthin chlorophyll alc binding protein, Fcpl F__---2Y ',-8077 13185 4.4 2.5OE-02 X99697.1 NT H.carterae mRNA for fucoxanthin chlorophyll alc binding protein, Fcpl' Page 49 of 196 3376 8519 13625 1.0 2.5OE-02 BE464190.1 EST HUMAN hx85d10.xl NCI CGAP Kidl 1 Homo sapiens cDNA clone IMAGE:3194611 3'similar to gb:M16938 HO5E0130-X PROTEIN HOX-C6 (HUMAN); 4111 9237 14320 5,1 2.5OE-02 AW592114.1 EST HUMAN hf36h08.xl Soares_NFL T GBC S1 Homo sapiens cDNA clone IMAGE:2934015 X 167 5361 10488 1.0 2.4OE-02 A1378582.1 EST-HUMAN to72c07.xl Soares Nhi- IMPu S1 Homo sapiens cDNA clone IMAGE:2070156 3' 1580 6706 11876 0.7 2.4OE-02 H65884.1 EST-HUMAN yr75fl 1.rl Soares fetal liver spleen 1 NFLS Horno sapiens cDNA done IMAGE:211149 5' 1998 7932 12316 0.8 2.4OE-02 P01901 SWISSPROT H-2 CLASS 1 HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H 2K(B)I) 1998 7932 12317 0.8 2.4OE-02 P01901 SWISSPROT H-2 CLASS 1 HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H 2K(B)) 4272 9397 14480 1.4 2.4OE-02 J051 10.1 NT T.thermophila caidum-binding 25 kDa (MBP 25) protein mRNA, complete cels 4420 9540 14631 1.4 2.4OE-02 P01901 SWISSPROT H-2 CLASS 1 HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H 2K(B)) 4420 9540 14632 1.4 2.4012-02 P01901 SWISSPROT H-2 CLASS 1 HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H 2K(B)) 5119 10215 11.5 2.4OE-02 AL161595.2 NT Arabidopsis thafiana DNA chromosome 4, config fragment No. 91 1829 6W8 2.1 2.3OE-02 W05340.1 EST_HUMAN za84g08xl Soares fetaijung NtiHI- 19W Horno sapiens cDNA done IMAGE:299294 5' 1844 6962 2.9 2.3OE-02 U94165.1 NT 4 Homo sapiens mammary tumorassoclated protein INT6 (INT6) gene, exon 4 2302 7405 12613 0.9 2.3OE-02 Z74293.1 NT S.cerevislae chromosome IV reading frame ORF YD1-245c 3628 8767 13861 4.8 2.3OE-02 Z20377.11 EST HUMAN HSAAACADH P, Human foetal Brain Whole tissue Homc, sapiens cDNA 4057 9184 14267 1.2 2.3OE-02 L24799.1 NT Gallus gallus connexin 45.6 (Cx45.6) gene, complete cds 4057 9184 14268 1.2 2.3012-02 L24799.1 NT Gallus gallus connexin 45.6 (Cx45.6) gene, complete cds' 4323 9446 14534 1.3 2.3OE-02 AW899107.1 EST-HUMAN CM4-NNO080-290400-160- b04 NNO080 Homo sapiens cDNA 4348 10 0-0 14561 0.9 2.3OE-02 AW593693.1 EST-HUMAN xs25d08.xl NCI_CGAP_Ut2 Homo sapiens cDNA clone IMAGE:2770671 3' 4348 10300 14562 0.9 2.3OE-02 AW593693.1 EST HUMAN xs25d08.xl NCI_PGAP U1:2 Homo sapiens cDNA done IMAGE:2770671 3, 4402 9523 14613 5.2 2.3OE-02 Z83867.1 NT Mycobacterium tuberculosis H37Rv complete genome; segment 1361162 4472 9592 14687 1.8 2.3OE-02 BE143150.1 EST HUMAN MRO-HT01 59.151099-001 - e03 HT01 59 Homo sapiens cDNA 4948 10053 15123 1,0 2.3OE-02 A1793177.1 EST HUMAN q45c01x5 NCI CGAP_Kidl 1 Homo saplens cDNA done IMAGE:2028868 & 4M 10053 15124 1.0 2.3OE-02 A1793177.1 EST HUMAN qz35c01x5 NCI CGAP_Kidl 1 Homo sapiens cDNA clone IMAGE:2028868 3, 720 5874 11014 1.1 2.2OE-02 AF018267.1 NT Columba livia nudeoside diphosphate kinase (NDPK) gene, nuclear gene encoding mitochondrial protein, complete cds 1713 6836 0.6 2.2012-02 4557448 NT Homo sapiens chromodoTain helicase DNA binding protein 2 (CHD2) mRNA 1968 7083 12284 0.6 2.2OE-02 Z82001.1 NT S.pneumoniae pcpA gene and open reading frames 2695 7952 12980 0.5 2.2OE-02 AF109633.1 NT Mus musculus ets variant protein ER81 gene, exons 1 through 4 3388 8531 1.9 2.2OE-02 AA577785.1 EST HUMAN rin24a04.sl NCl_piGAP Gasli Homo sapiens cIDNA done IMAGE:1 084782 3' 3595 8734 3. 3 2.2OE-02 AF083094.11 NT Infectious bursal disease virus segment B strain IL4 VP1 gene, complete cds 3779 8916 14009 1.1 2.2OE-02 AW601317.1 EST HUMAN PM0-13T0340-170100-004- b03 BT0340 Homo, sapiens cDNA 3844 8980 14081 1.0 2.2OE-02 Z74293.11 NT S.cerevislae chromosome W reading frame ORF YD1.245c 441 5607 2.7 2.1OE-02 AF029726.11 NT Dictyostelium discoideum hisfidine kinase C (dhkC) mRNA, complete cds L_1r--- 6371 11530 2.5 2.1012.02 U72073.1 NT Bacillus subtills cotKI-M duster, CoK (c6tK), Coti. (cotL), and spore coat protein ColM (cotM) genes, complete eds Page 50 of 196 Table 4

1365 6492 11657 0.7 2.1OE-02 AF204395.1 NT Mus musculus macrophage migration Inhibitory factor (MIF) gene, 5'flanking region and partial cds 1365 6492 11658 0.7 2.1 OE-02 AF204395.1 NT Mus musculus macrophage migration inhibitory factor (MIF) gene, 6flanking region and partial cds 1920 7037 12226. 1.0 2.1 OE-02 AF190899,1 NT Tegula aureofincta major acrosomal protein precursor (TM P) mRNA, complete cds 2785 5915 11062 1.4 2.11OE-02 N29266.1 EST_HUMAN yx43h07xl Soares melanocyte 2PN_bHM Homo sapiens cDNA done IMAGE:264541 5' 3110 7105 12308 0.9 2.1OE-02 BE072546.1 EST HUMAN PM2-13T0546-1201 00- 001411 BT0546 Homo sapiens cDNA 3110 7105 12309 0.9 2.10E,02 BE072546.1 EST-HUMAN PM2-13T0546-1201 00- 001411 BT0546 Horno sapiens cDNA 3535 8676 13779 1.2 2.1OE-02 AA461271.1 EST-HUMAN zx63b09xl Soares-totai- fetus_Nb2HF_9w Homo sapiens cDNA clone IMAGE:796121 5, 4038 9166 14251 0.9 2.1OE-02 Z74293.1 NT S.cerevislae chromosome IV reading frame ORF Y131-245c 4342 9465 14554 1.6 2.1OE-02 U44914.1 NT Borrelia burgdorfed plasmid cp32- 2, erpC and erpl) genes, complete cds; and unknown genes 4351 9473 14565 1.0 2.1 OE-02 A1768127.1 EST-HUMAN wg81 dl 1.xl Soares_NSF_F_9VV_OTPkP_Sl Homo sapiens cIDNA clone IMAGE:2371509 3' 4611 9725 14820 4.7 2.1 OE-02 Y08501.1 NT A.thallana mitochondrial genome, part A 4714 9826 1.4918 0,9 2.1OE-02 A1823432.1 EST-HUMAN wh54a05.xl NCI CGAP_Kidl 1 Homo sapiens cDNA done IMAGE:2384528 3' 18 5227 10328 2,7 2.OOE-02 AW895565.1 EST HUMAN QV4-NNO038-270400-187-h05 NNO038 Homo saRiens cIDNA 259 5447 10572 0.9 2.OOE-02 6753635 NT Mus musculus DInB homolog 1 (E. coli) (Dinbl), mRNA 292 5478 10609 0.9 2.OOE-02 AA456538.1 EST-HUMAN aa15bl 0A Soares NhiIMPu S1 Homo saplens cDNA clone IMAGE:813307 5 781 5933 11082 0.6 2.OOE-02 6753635 NT Mus musculus DinB homolog 1 (E. coli Inbl), mRNA 1071 6209 11362 1.0 2.OOE-02 AL096805.1 NT Homo sapiens genomic region containing hypervarlable minisatellites chromosome 1 [1 p36.33] of Homo sapiens 1181 6314 11470 0,6 2.OOE-02 8922391 NT Horno sapiens hypoth6Ucal protein FLJ10379 (FLA0379), mRNA 1181 6314 11471 0.6 2.OOE-02 8922391 NT Homo sapiens hypothetical protein FLJ10379 (FLA0379), mRNA 1830 6949 12132 0.8 2.OOE-02 8R2-453 NT Horno, sapiens hypothetical protein FLJ10486 (FLA0486), mRNA 1830 6949 12133 0.8 2.OOE-02 8922453 NT Homo sapiens hypothetical protein FLJ10486 (FLA0486), mRNA 2768 7852 0.6 2.00IE-02 AL1 61532.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 32 3105 8255 1.2 2.OOE-02 7305474 NT Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 613 (Sema6b), mRNA 3189 8338 3,3 2.OOE-02 AF095588.1 NT Arabidopsis thallana C2H2 zinc finger protein FZF MRNA, complete cds 3925 9059 14146 1.5 2.OOE-02 M18095.1 NT P.vulgads hydroxyprollne-rich glycoprotein (HRGP) mRNA, 3' end 5079 10177 15257 1.0 2.OOE-02 ALl 63278.2 NT Homo sapiens chromosome 21 segment HS21C078 5085 10183 15262 1.0 2.OOE-02 AA456538.1 EST-HUMAN aa15bl 0A Soares-Nhi- IMPu S1 Horno saplens cDNA done IMAGE:813307 9 678 5833 10964 0.6 1.9012-02 AA572764.1 EST HUMAN nf19a07.sl NCICGAP_Prl Homo saplens cIDNA done IMAGE:914196 similar to contains L1A1 Ll repetitive element; 1599 6726 11896 0.8 1.90E-02 P18488 SWISSPROT EMPTY SPIRACLES HOMEOTIC PROTEIN 1993 7109 12311 0.9 1.9OE-02 AL163303.2 NT Homo sapiens chromosome 21 segment HS21 C103 1993 7109 12312 0.9 1.9OE-02 AL163303.2 NT Homo sapiens chromosome 21 segment HS21 Cl 03 2458 7557 12762 0.9 1.9OE-02 AL161550.2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 50 8026 13128, 7.1 1.9OE-02 AA713856.1 EST-HUMAN nw0005.sl NCI CGAP SS1 Homo, sapiens cDNA done IMAGE:1238337 3' Page 51 of 196 Table 4

3222 8370 0.8 1.9OE-02 AB03361 1,1 NT Urotrichus talpoides mitochonddal gene for cytochrome b, complete eds 3560 8699 1.3 1.9OE-02 N52250.1 EST HUMAN yz28b02.sl Scares - mulfiple- scierosip_2NbHMSP Homo sapiens cDNA clone IMAGE:284331 3' 3654 8793 13887 1.0 1.9012-02 A1301183.1 EST-HUMAN qn04c07.xl NCI-CGAP- Lu5 Homo sapiens cIDNA clone IMAGE:1897260 3' similar to contains Alu repetitive element; 3961 9093 14178 1.1 1.90E-02 AF141940.1 NT Mycoplasma Imitans VibAl precursor (vihAl) and VihA2 precursor (vihA2) genes, partial cds 4099 9225 14309 1.5 1.9OE-02 P09081 SWISSPROT HOMEOTIC BICOID PROTEIN (PIRD-4) 4099 9225 14310 1,5 1.90E-02 P09081 SWISSPROT HOMEOM BICOID PROTEIN (PRD- 4) 4441 9561 14657 2.5 1.90E-02 A1452999.1 EST-HUMAN 1J46d04.xl Soares-NSF- _OT_PA-P-SI Homo sapiens cDNA done IMAGE:2144551 Ysimilar to contains Alu repetitive element; 4944 7557 12762 1.9 1.90E-02 AL161550.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 50 5291 1.1 1.8OE-02 09Z9HO SWISSPROT [PROTEIN-PI1] URIDYLY1-TRANSFERASE (P11 URIDYM-TRANSFERASE) (URIDYLYL REMOVING ENZYME) (UTASE) 343 5524 10649 1.4 1.8OE-02 AW771104.1 EST HUMAN hn52c06.xl NCI - CGAP - Co17 Homo sapiens cDNA clone IMAGE:3027274 Ysimilar to contains element MERS repetitive element; 1144 6279 11432 1.3 1.8OE-02 X17664,1 NT H.francisd mRNA for myelin basic protein (MBP) 1417 6W 11719 1.2 1.8OE-02 AF243382.1 NT Drosophila melanogaster cytopla mic protein encore (enc) mRNA, complete cds 3174 8323 0.9 1.8OE-02 A1805829.1 EST HUMAN te52a09.xl Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE:2090296 3' 3489 8630 13742 1.1 1.8OE-02 Z75059.1 NT S.cerevislae chromosome XV reading frame ORF YOR1 51 c 3812 8949 14045 1.1 1.8OE-02 AW879122.1 EST-HUMAN MR1 -OT001 1-280300-009- 904 OT001 1 Horno sapiens cDNA 3812 8949 14046 1,1 1.8OE-02 AW879122.1 EST HUMAN MR1-OT001 1-280300-009. 904 OT001 1 Homo sapiens cDNA 3996 9128 1.2 1.8OE-02 AA861446.1 EST-HUMAN W404.sl Soares tesfis_NHT Homo sapiens cDNA done IMAGE: 1406935 3, 4332 F455 14543 1.4 1.8OE-02 AW936363.1 EST HUMAN QV4-DTQ021-301299-071- bll DT0021 HomosapienscDNA 4873 9980 15064 1.1 1.8OE-02 060810 SWISSPROT HYPOTHETICAL PROTEIN DJ845024.2 4887 9994 15075 1.1 1.8OE-02 A1288701.1 EST HUMAN gm06b04.xl NCI CGAP Lu5 Homo sapiens cDNA done IMAGE:1881007 3' 888 6036 11194 1.3 1.70E-02 BE394869.1 EST-HUMAN 601310626F1 NIH MGC 44 Homo saplens cDNA clone IMAGE:3632190 5' 1750 6872 12051 0.8 1.70E-02 AW573183.1 EST-HUMAN M34a03.xl Scares - NFL T - GBC S1 Homo sapiens cDNA done IMAGE:2933740 3'similar to contains L1.0 L1 repetitive element; 1750 6872 12052 0.8 1.70E-02 AW573183.1 EST HUMAN M34a03.xl Soares-NFL-T- GI3C-S1 Homo sapiens cDNA clone IMAGE:2933740 Ysimilar to contains LIA1 L1 repetitive element; 1828 6W7 1.1 1.70E-02 AL163204.2 NT Honno sapiens chromosome 21 segment HS21 C004 2066 71 8 4.7 1.70E-02 A13004816.1 NT O!Ictolagus cufficulus mRNA for mitsugumin29, complete cds 2603 7693 1.4 1.70E-02 7657495 NT Homo sapiens putative Rab5 GDPIGTP exchange factor homologue (RABEX5), mRNA 2968 8119 13227 0,91.70E-02 A1147615.1 EST HUMAN qb22a08.xl SoarespregnanLuterus-NbHPLI Homo sapiens cDNA clone IMAGE: 1696982 3, 3467 8608 4.2 1.70E-02 AW827368.1 EST-HUMAN hm45a04.xl NCI CGAP RDFl Homo saplens cDNA done IMAGE:3015534 Ysimilar to contains MER1911 MER19 repetitive element; 3573 8712 0.9 1.70E-02 P04929 SWISSPROT HISTIDINE-RICH GLYCOPROTEIN PRECURSOR 9205 1.0 1.70E-02 AA669618.1 ESTHUMAN ac19f04.sl Stratagene ovary (#937217) Homo sapiens cDNA done IMAGE:856927 X similar to contains Alu repetitive element;contalns element MER24 repetitive element; Page 52 of 196 Table 4

4105 9231 1.8 1.70E-02 R02506.11 EST-HUMAN ye86f08xl Scares fetal liver spleen INFLS Homo saplens cDNA done IMAGE:124647 5' 4427 9548 14641 1.2 1.70E-02 AW573183.1 EST-HUMAN W34a03.xl Soare!_NFLGBC_Sl Homo sapiens cDNA cJone IMAGE:2933740 X similar to contains Ll.tl L1 repetitive element:

4615 9729 14823 1.8 1.70E-02 V00641.1 NT Messenger RNA for anglerfish (Lophius amoricanus) sornatostatin fl 4708 9820 5.3 1.70E-02 A1015076.1 EST-HUMAN ov51c02.sl Soares tesds NHT Horno sapiens cDNA done IMAGE:1640858 3, 4967 10070 15144 5.5 1.7OE-02 AF105037.1 NT Murfd herpesvirus 4 complete genome 501 5665 0.5 1.6OE-02 AL021929.1 NT Mycobacterium tuberculosis H37Rv complete genome; segment 131162 1635 6761 11931 1.1 1.6012-02 Y18889.1 NT Treponema mattophilum flaB2, flaB3 and fill) genes for flagellin subunit proteins and CAP protein homologue 2202 7311 12522 1.1 1.60E-02 Q64176 SWISSPROT LIVER CARBOXYLESTERASE 22 PRECURSOR (EGASYN) (ESTERASE-22) 2202 7311 12523 1.1 1.6OE-02 Q64176 SWISSPROT LIVER CARBOXYLESTERASE 22 PRECURSOR (EGASYN) (ESTERASE-22) 2518 7614 12816 1.0 1.60E-02 AJ006345.1 NT Homo sapiens WWT1 gene w 2606 7696 12900 0.5 1.60E-02 AA484872.1 EST-HUMAN ne8ldO6.sl NCI_PGAP_Ewl Horno sapiens cDNA clone IMAGE:91 0667 2662 7751 1.1 1.60E-02 AB014534.1 NT Homo sapiens mRNA for KIAA0634 protein, partial ods 3481 8622 13734 3.8 1.6OE-02 AW850652.1 EST_HUMAN IL3-CTO219-160200-063- CO7 CTO219 Homo saplens cDNA 4084 9211 2.2 1.60E-02 AF1 10520,1 NT Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, WC1, Fas-binding protein, BING1, tapasin, RaIGDS- like, KE2, BING4, beta 1,3-galactosyl transferase, and RPS18 genes, complete ods., Sacm21 gene, partial> 4203 9328 14417 0.9 1.60E-02 AW875407.1 EST_HUMAN W2-PT001 2-140100- 030407 PT0012 Homo sapiens cDNA 734 5888 8.5 1.5OE-02 8923734 NT Homo sapiens transcription factor (HSA130894), mRNA 2095 7206 12411 1.4 1.50E-02 N395211.1 EST-HUMAN yv27b07.sl Soares fetal liver spleen 1 NFLS Horno sapiens cDNA cjone iMAGE:243925 3, 2128 7238 12446 0,9 1.5OE-02 AL161594.2 NT Arabldopsis thallana DNkchromosome 4, config fragment No. 90 3023 8175 13275 1.4 1.50E-02 AJ006216.1 NT Homo sapiens CACNA1 F gene, exons 1 to 48 3023 8175 13276 1.4 1.50E-02 A.1006216.1 NT Homo sapiens CACNA1 F gene, exons 1 to 48 3614 8753 1.2 1.5OE-02 AA458797.1 EST-HUMAN aa88c110.s I Stratagene fetal retina 937202 Homo saplens eDNA clone IMAGE:838386 3' 4049 9176 14259 1.2 1.50E-02 AA160967.1 EST---HUMAN zq4091O.rl Stratagene hNT neuron (#937233) Homo sapiens cDNA done IMAGE:632226 5, 411 -5579 1.5 1.40E-02 AE002230.2 NT Chtarnydophila pneumoniae AR39, section 58 of 94 of the complete genome 1102 6240 11392 1.2 1.40E-02 7705980 NT woteln (LOC51225), mRNA Homo saplens NESH p 1236 6365 1.4 1.40E-02 U32800.1 NT Haernophilus Influenzae Rd section 115 of 163 of the complete genome 1276 6406 1.0 1.40E-02 U67779.1 NT Xenopus laevis neurogenin related 1 b (X-WNRA b) mRNA, complete cds 1374 6502 0.9 1.40E-02 AF216854.1 NT H6mo sapiens headpin gene,:complete cds 3352 8496 13606 1.0 1.40E-02 AW074212.1 EST-HUMAN xbOgdOg.xl NCI CGAP_GU1 Homo saelens cDNA clone IMAGE:2575793 3' 3439 8581 13693 5.2 1.40E-02 AL161586.2 NT Arabidopsis thaliana DNA chromosome 4. oon#g fragmenkNo. 82 3439 8581 13694 5.2 1.40E-02 A1.161586.2 NT Arabidopsts thaliana DNA chromosome 4. config fragment'No. 82 3476 8617 13729 1.0 1.40E-02 4503628 NT Homo saplens coagulation factor XII (Hageman factor) (F1 2), mRNA 8745 13840 6.2 1.40E-02 6996918 NT Mus musculus histocompatibillity 2, complement component factor B (1-12-13% mRNA Page 53 of 196 Table 4

4387 9509 14594 7.3 1.40E-02 AW962688.1 EST HUMAN EST374761 MAGE resequences, MAGG Homo sapiens cDNA 4387 9509 14595 7.3 1.40E-02 AW962688.1 EST-HUMAN EST374761 MAGE resequences, MAGG Horno, sapiens cDNA 1912 7029 ' 12216 0.9 1.30E-02 AL163201.2 NT Homo sapiens chromosome 21 segment HS21 C001 3888 9024 1.4 1.30E-02 AF169288.1 NT Mus musculus beta-sarcoglycan gene, complete ods 5176 7029 12216 1.0 1.30E-02 AL1 63201.2 NT Homo saplens chromosome 21 segment HS21 C001 206 5399 10660 0.9 1.2OE-02 X87344.1 NT H.sapiens DMA, DIVIB, HLA21, IPP2, LIVIP2, TAP1, LIVIP7, TAP2, DOB, DQB2 and RING8, 352 5532 0.6 1.20E-02 AA059299.1 EST-HUMAN 9, 13 and 14 genes zf65g01xl Soares retina N2b4HR Homo saplens cIDNA clone IMAGE:381840 Wsimilar to contains element L1 repetitive element; 445 5611 10742 1.5 1.20E-02 P38898 SWISSPROT HYPOTHETICAL 17.1 KID PROTEIN IN PURS YREGION 721 5875 11015 1.0 1.20E-02 A1183522.1 EST HUMAN qd68e12.xl Soares_testis- NHT Homo sapiens cIDNA done IMAGE:1734670 3' similar to contains Ll.tl L1 repetitive element:

2130 7240 12448 0.7 1.20E-02 AL163213.2 NT - Homo sapiens chromosome 21 segment HS21C013 2393 7493 12699 1.2 1.20E-02 AW172350.1 EST HUMAN xj37e09.xl Scares NFL, GBC S1 Homo saplens cDNA clone IMAGE:2659432 3' 2596 7493 12699 1.3 1.20E-02 AW172350.1 EST-HUMAN xj37e09.xl Soares NFLj_GBC SI Homo sapiens cIDNA. clone IMAGE:2659432 X 3063 8214 6.2 1.20E-02 AA075418.1 EST HUMAN zm88e03.rl Stratagene ovarian cancer (#937219) Homo sapiens cIDNA clone IMAGE:545020 5' 3250 8397 13503 1.9 1.20E-02 R62805.1 EST HUMAN yj11b08.sI Soares placenta Nb2HP Homosaplens cDNAdone IMAGE:138903 X 4830 9938 15027 2.0 1.20E-02 U91328.1 NT Human hereditary haemochromatosis region, histone 2A-like protein gene, hereditary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds 4974 10077 1.7 1.20E-02 AB019786.1 NT Cynops pyrrhogaster CpUbiqT mRNA, partial cds 1248 6377 11536 1.3 1. 1 OE-02 AA070364.1 EST HUMAN zm69el.l.sl Stratagene neuroepithelium (#937231) Homo apiens cIDNA clone IMAGE:530924 3' 1679 6803 11983 0.7 1.1OE-02 X75491.1 NT H.sapiens LIPA gene, exon 4 1679 6803 11984 0,7 1.1OE-02 X75491.1 NT H.sapiens LIPA gene, exon 4 2848 7999 3.5 1. 1 OE-02 N99523.1 EST HUMAN za40e05xl Scares fetal liver spleen 1 NFLS Homo saplens cIDNA clone IMAGE:295040 5' 2933 8084 13192 10.4 1.1OE-02 AF055066.1 NT Homo sapiens WC class 1 region 3478 8619 13732 2.5 1.1OE-02 A1653508.1 EST-HUMAN tq95b10.xl NCI - CGAP Ov23 Horno, sapiens cDNA done IMAGE:2216539 Ysimilar to SW:WF-HUMAN Q92i89 ONA.1REPAIR PROTEIN COMPLEMENTING XP-F CELL; 3937 9071 0.9 1.1OE-02 BE144637.1 EST HUMAN PM3-HT0175-300999-001-h06 HT0175 Horno sapiens cDNA 4016 9146 1.0 1.1OE-02 AW813796.1 EST HUMAN RC3-ST01 97-120200-015-911 ST01 97 Homo sapiens cDNA 4723 9835 14927 1.8 1.1OE-02 AL048383.2 EST-HUMAN DKFZp586E0924-sl 586 (synonym: hutel) Homo sapiens cIDNA clone DKFZp586E0924 4821 9930 0.9 1.1OE-02 AW820281.1 EST HUMAN QV2-ST0296-150200-028-cl 1 ST0296 Homo sapiens cDNA 6 5214 10317 1.1 1.OOE-02 AW846120.1 EST HUMAN MR3-CT0176-111099-003-e10 CTO176 Homo sapiens cIDNA 1506 6633 11802 0.9 1.OOE-02 AW368128.1 EST-HUMAN C1V12-HT017-7-04109"17- h12 HT0177 Homo sapiens cDNA 2525 7621 1.2 1.0OE-02 AA806389.1 EST HUMAN oc22h08.sl NCI WAP GC131 Horno sapiens ONA done IMAGE:1 350495 3' 3464 8605 1 0 1.OOE-02 AW845621.1 EST HUMAN MRO-CTO060-081099-003-h10 CTO060 Horno, sapiens cDNA 8942 140371 0.8 1.0OE-02 A1065086.1 EST HUMAN HA0921 Human fetal liver cIDNA library Homo sapiens cIDNA Page 54 of 196 Table 4

4667 9779 14877 4.3 1.OOE-02 6753521 NT Mus musculus cordcotropin releasing one receptor 2 (Crhr2), mRNA 4736 9847 14939 2.4 1.OOE-02 R96567.1 EST HUMAN yq54h01xl Soaresfetal liverspleen 1NFI-S Homosapienscl3NAdone IMAGE:1996335' 4995 10098 15165 0.9 1.0OE-02 AF218910.1 NT Homo sapiens attractin precursor (ATRN) gene, exon 25 and complete ods, alternatively spliced 875 6023 11183 0.6 9.OOE-03 A1796126.1 EST-HUMAN wh42f09.xl NCI CGAP - Kidl 1 Homo sapiens cIDNA cJone IMAGE:2383433 Ysimilar to contains elemeit MER22 MER22 repetitive element. 1 2346 7448 12655 1.2 9.OOE-03 AL161559.2 NT Arabidopsis thallana DNA chromosome 4, config fragment No. 59 2593 7685 12887 1.1 9.OOE-03 AJ243727.1 NT Oncorhynchus nerka proviral gypsy retrotransposon partial reverse transcriptase and protease genes (pol gene) 2593 7685 12888 1.1 9.OOE-03 AJ243727.1 NT Onoorhynchus nerka proviral gypsy retrotransposon partial reverse transcriptase and 2876 8027 13129 0.8 9.OOE-03 A1251744.1 EST-HUMAN protease genes (pol gene) 2876 8027 13130 0.8 9.OOE-03 A1251744.1 EST HUMAN qh90f09.xl Soares NFL, GBC S1 Homo saplens cDNA done IMAGE:1854281 3' gh90f09.xl Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE:1854281 3' 3617 8756 13849 0.9 9.OOE-03 J05184.1 NT S.addocalclarlus thermoLsin gene, complete eds 4914 10021 15100 1.2 9.OOE-03 BE047949.1 EST-HUMAN tz44e1O.yl NCI CGAP 13m52 Homo saplens cDNA done IMAGE:2291466 5' 5181 10273 2.6 9.OOE-03 AF137240.1 NT Sargocentron sp. mixed lineage leukemia-like protein (M11) gene, partial ods 493 5658 1.1 8.OOE-03 AA723007.1 EST HUMAN zh30e03.sl Soares_pineal_gland N3HPG Homo sapilens cDNA done IMAGE:413596 3 similar to contains Alu repetitive itement; 973 6119 11276 15.0 8.OOE-03 AF106656.1 NT Horno sapiens adenylosuccinate Iyase gene, complete ods 2113 7224 12432 0.9 8.OOE-03 AL163283.2 NT Homo sapiens chrom some 1 segment HS21 C083 2930 8081 0.9 8.OOE-03 U47048.1 NT Escherichia coil microcin 24 region, DNA binding protein (mdbA), immunity protein (mtfi), microcin 24 (mtfS). and microcin transport protein (mtIA, mtfB) genes, complete cds 3270 8415 13522 1.0 8.OOE-03 BE171225.1 EST HUMAN RC1-HT0545-120200-01 1- b09 HT0545 Homo saplens cDNA 3314 8459 13571 0.9 8.0012-03 AJ131016.1 NT Homo sapiens SCL gene locus 3792 8929 1.1 8.OOE-03 AF058764.1 NT Xenopus laevis bone morphogenefic protein 4 (BIVIP-4) gene. complete cels 679 5834 10965 3.9 7.OOE-03 AF097183.1 NT Cryptosporidium parvum HC-10 gene, complete cds 679 5834 10966 3.9 7.OOE-03 AF097183.1 NT CryptosRoddlum parvum HC-10 gene, complete cds 1341 6469 1.2 7.OOE-03 Q61060 SWISSPROT FORKHEAD BOX PROTEIN D3 (HNF3/FH TRANSCRIPTION FACTOR GENESIS) (HEPATOCYTE NUCLEAR FACTOR 3 FORKHEAD HOMOLOG 2) (HFH-2) 1373 6501 11667 5,0 7.00IE-03 AA668298.1 EST HUMAN ab79b09.sl Stratagene fetal retina 937202 Homo saplens cIDNA clone IMAGE:853145 3 1486 6613 11783 1,7 7.OOE-03 AW303599.1 EST-HUMAN xv21 b02.xl Soares NFL T GBC S1 Homo sapiens cDNA done IMAGE:2813739 3' 2210 7937 12533 0.8 7.OOE-03 P04929 SWISSPROT HISTIDINE-RICH GLYCOPROTEIN PRECURSOR 3127 8276 13382 1.9 7.0012-03 AW503321.1 EST HUMAN UI-I-IF-BNO-akd-03-0- Ul.rl N11-1-1VIGQ_50 Homo sapiens cDNA done IMAGE:j078580 5' 3702 8841 13932 1.3 7.OOE-03 AW444463.1 EST HUMAN UJ-1-1-1313-akb-c-1 0-0- Ul.sl INICI-CGAPS05 Homo saplens cDNA done IMAGE.2733691 3' 8878 13968 1.0 7.OOE-03 AF196344.1 NT Rattus norvegicus neuronal nicotinic acetylcholine receptor subunit (A)phalO) mRNA, complete cds Page 55 of 196 1 Table 4 j d- 4434 9555 1.2 7.OOE-03 AW117711.1 EST HUMAN xe34f09.xl NCI - WAP_Uti Homo sapiens cDNA done IMAGE:2609033 Ysimilar to TR:Q12987 Q12987 ACIDIC 82 KDA PROTEIN.; 4495 9615 1.0 7.OOE-03 AW630888.1 EST HUMAN hh89a05.yll NCI_PGAP_GUll Homo sapiens cDNA done IMAGE:2969936 5' 4,916 10023 1.8 7.OOE-03 AL163278.2 NT Homo sapiens chromosome 21 segment HS21 C078 5038 10138 15215 1.1 7.OOE-03 BE044191.1 EST HUMAN ho39h08.xl Soares--- NIFL-T-GI3C-S1 Homo saplens cDNA done IMAGE:3039807 Ysimilar to TR:093434 093434 RETICULOCALBIN., 5038 10138 15216 1.1 7.OOE-03 BE044191.1 EST-HUMAN ho39h08.xl Soares_N17k3_WC-S1 Homo sapiens cONA done IMAGE:3039807 3'simiiar to TR:093434 093434 RETICULOCALBIN.; 1220 6351 11511 3.2 6.OOE-03 AW511148.1 EST-HUMAN W22a05.xl Soares_NFL-T- GWS1 Homo sapiens cDNA done IMAGE:2910224 Ysimilar to SW:PXR HUMAN 075469 ORPHAN NUCLEAR RECEPTOR PXR; 1220 6351 11512 3.2 6.0012-03 AW511148.1 EST-HU-MAN W22a05.xl Soares-NFL. T_GBC - 51 Homo sapiens cDNA done IMAGE:2910224 Ysimilar to SW:PXR HUMAN 075469 ORPHAN NUCLEAR RECEPTOR PXR; 2744 7828 13031 1.1 6.OOE-03 AF1 12374,1 NT Danlo redo;dorant recept& gene duster 2862 8013 13113 3.1 6.OOE-03 AA759135.1 EST HUMAN ah78el I.sl Soares tests NI-IT.Homo sapiens cDNA done 1321772 3, 2862 8013 13114 3.1 6.OOE-03 AA759135.1 EST-HUMAN ah78el 1.sl Soares tesds_NHT Horno saplens cDNA done 1321772 3' 3208 8356 2.1 6.OOE-03 H75690.1 EST-HUMAN yr77h04xl Soares fetal liver spleen 1 NFLS Homo.sapiens cDNA clone IMAGE:211351 V 3336 8480 13594 1.2 6.OOE-03 U90880.1 NT Fugu rubripes zJnc finger protein, isotocin, fatty acid binding protein, sepiapterin reductase and vasotodn genes, complete cds 3336 8480 13595 1.2 6.OOE-03 U90880.1 NT Fugu rubripes Mc finger protein, isotocin, fatty acid binding protein, sepiapterin reductase and vasotocin genes, complete cds 3499 8641 1.1 6.OOE-03 W37985.1 EST HUMAN zcl 3al 1xl Soates_parathyroldtumor-NbHPA Homo sapiens cDNA clone 1 MAGE:322172 5' 3711 8849 13939 1.3 6.OOE-03 6754029 NT Mus musculus glucosamine-6- phosphate deaminase (Gnpi), mRNA 3846 8982 14083 0.9 6.OOE-03 AW847284.1 EST-HUMAN RCO-CT0204-240999-021- b10 CT0204 Homo sapiens eDNA 3873 9009 1.2 6.OOE-03 BE250108.1 EST-HUMAN 600942904F1 N1lrlpGCJ5 Homo sapiens cDNA done IMAGE:2959513 5 4231 9355 0.9 6.OOE-03 N58946.1 EST HUMAN yy62h1O.sl Soares_mulfiple- scJerosis_2NbHMSP Homo sapiens cDNA clone IMAGE:278179 3' 4267 9392 1.4 6.OOE-03 A1016833.1 EST HUMAN ov33j1 1.xl Soares testis NHT Homo saplens cDNA done IMAGE:1639124 3' 4597 9711 14803 6.8 6.OOE-03 AA324242.1 EST HUMAN EST27116 Cerebellum 11 Homo sapiens cDNAT end stmilarto EST containing urepeat 5115 10211 15290 1.8 6.OOE-03 AL163281.2 NT Homo sapiens chromosome 21 segment HS21 C081 5129 10224 15299 0.9 6.OOE-03 AA889972.1 EST HUMAN aj95g09.sl Soares_parathyroid-tumor-NbHPA Homo sapiens cDNA clone IMAGE:1404256 3' 653 5811 10938 0.7 5.OOE-03 L25105.1 NT Chlamyclia trachornatis partial ORFB; aminoacyl-tRNA synthase, complete eds; complete ORFA, and grpE-like protein, complete ods 653 5811 10939 0.7 5.OOE-03 L25105.1 NT Chlamydia trachomads partial ORFB; aminoacyi-tRNA synthase, complete cds; complete ORFA, and grpE-like protein, complete cds 654 5811 10938 1.0 5.OOE-03 L25105,1 NT Chlamyclia trachomatis partial ORFB; aminoacyt-tRNA synthase, complete cds; complete ORFA, and grpE-like protein, complete cds 5.OOE-03 L25105.1 NT Chlamydia trachomads partial ORFB; aminoacyl-tRNA synthase, complete ods; complete ORFA, and grpE-like protein, complete ods Page 56 of 196 Table 4

1096 6234 11386 1.4 5.OOE-03 AJ010457.1 NT Arabido is thaliana mRNA for DEAD box RNA helicase,RH3 1115 6252 11402 1.3 5.0012-03 AW293363.1 EST HUMAN U1-1-1-1312-ahl-d-06-0- Ul.sl NCLWAP-Sub4 Homo sapiens cDNA clone IMAGE:2727203 3, 1115 6252 11403 1,3 5.OOE-03 AW293363.1 EST-HUMAN U1-1-1-1312-ahl-d-O"-Ul. sl NCLWAP-Sub4 Homo sapiens cDNA done IMAGE:2727203 X 1552 6679 0.9 5.0012-03 A1138977.1 ESTHUMAN qd79d05.xl Soares_testis_NI- IT Homo sapiens cDNA done IMAGE: 1735689 3' 2648 7737 12937 1.2 5.0OE-03 AB033006.1 NT Homo saplens mRNA for K1AA1 180 protein, partial cds 2905 8056 13161 1.0 5.OOE-03 RE266057.1 ESTHUMAN 601194796F1 NIH MGC 7 Homo sapiens cDNA done IMAGE:3538799 5' 3097 8248 13352 4,1 5.OOE-03 T87623.1 EST HUMAN yc81f09.sl Scares Infant brain 1 NIB Horno sapiens cDNA clone IMAGE:22395 3' 3116 8265 1.8 5.0OE-03 AL161491.2 NT Arabidopsis thallana DNA chromosome 4, contiq fragment No. 3 3129 8278 13385 1.0 5.OOE-03 R71794.1 EST-HUMAN yj86g02.sl Scares breast 2NbHBst Homo sapiens cDNA done IMAGE: 155666 3' 3240 8387 1.1 5.OOE-03 AJ297357.1 NT Horno sapiens partial LIMD1 gene for LIM domains containing protein 1 and KIAA0851 gene 3641 8780 13873 4,2 5.OOE-03 AF147449.2 NT Pseudomonas aeruginosa strain PA01 penidilin-binding protein 18 (ponB) gene, complete cds 3698 8837 13928 0.8 5.0OE-03 U38914.1 NT Citrus sinensis seed storage protein dtrin mRNA, complete cds 3890 9026 1.4 5.OOE-03 AA299675.1 EST HUMAN EST1 2218 Uterus tumor 1 Horno, sapiens cDNA Send 4207 9332 14418 0.9 5.OOE-03 H78355.1 EST HUMAN yu79g10xl Soares.fetal liver spleen I NFLS Homo sapiens cDNA done IMAGE:240066 5, 4209 8837 13928 0.9 5.OOE-03 U38914.1 NT Citrus sinensis seed storage protein dtrin mRNA, complete ods 4503 9622 14713 0.9 5.OOE-03 AJ131016.1 NT Homo sapiens SCL gene locus 4619 9733 14828 2.5 5.OOE-03 A1752367.1 ESTHUMAN w15c02,xl Normal Human Trabecular Bone Cells Homo sapiens cDNA done NI-ITBC-cri15c02 random 232 5423 10547 1.0 4.OOE-03 AW500196.1 EST-HUMAN Ul-HF-BNO-akch-04-0-Ut. rl N11-1-MGQ_50 Homo sapiens cDNA done IMAGE:3076831 9 318 5502 10629 0.7 4.0012-03 R46482.1 EST HUMAN y951 e04.sl Soares Infant brain 1 NIB Homo sapiens cDNA done IMAGE:35988 31 436 5602 10735 0.9 4.OOE-03 P54675 SWISSPROT PHOSPHATIDYLINOSITOL 3- KINASE 3 (P13-KINASE) (PTDINS-3-KINASE) (P13K) -588 5748 10865 1.0 4.0012-03 AA939339.1 EST-HUMAN on75912.sl Soares,_NFL T GBC S1 Horno sapiens cDNA done IMAGE:1562566 3' 859 6007 11169 0.6 4.OOE-03 R46482.1 EST HUMAN yg51e04.sl Soares infant brain I NIB Homo sapiens cDNA done IMAGE:35988 3' 893 6041 1.2 4.OOE-03 AW749101.1 EST-HUMAN RC3-BT0333-110100-012-f01 BT0333 Horno sapiens cDNA 1133 65-9 11421 11.0 4.OOE-03 AA099777.1 EST HUMAN z181a08.rl Stratagene colon (#937204) Homo saRiens cDNA clone IMAGE:510998 5, 1153 6286 11442 0.5 4.OOE-03 AW794740.1 EST-HUMAN IRC6-UM0014-170400-023- GO1 UM0014 Homo saplens cDNA 1281 6411 11572 1.3 4.OOE-03 AA284374.1 EST HUMAN zs59a01xl NCI_PGAP_GCS1 Homo sapiens cDNA done IMAGE:701736 5' 1709 6832 12012 0.8 4.OOE-03 U33472.1 NT Rattus norvegicus type 1 ashe and olfactory-limbic associated protein AT1-46 mRNA, complete cds 1971 7086 12289 7.6 4.OOE-03 AA099777.1 EST-HUMAN A81a08.rl Stratagene colon (#937204) Homo sapiens cDNA clone IMAGE:510998 5' 2200 7309 0 6 4.OOE-03 BE410556.1 EST HUMAN 601304161 F1 N1H MGCj21 Homo saplens cDNA done IMAGE:3638510 5, 2232 51 0.5 AW794740.1 ESI_HUMAN IRC6-1.1M0014-170400-023-GO1 UM0014 Homo sapiens d3NA 1 C.

Page 57 of 196 Table 4 t>o d- 2526 7622 12822 0.7 4.OOE-03 U52111.2 NT Homo sapiens X28 region near ALD focus containing dual specificity phosphatase 9 (DUSP9), ribosomaf protein Ll 8a (RPLI 8a), Ca2+1Calmodulin-dependent protein kinase 1 (CAMKI), creatine transporter (CRTR), CDM protein (CDM), adrenoleukodystrophy protein > 2526 7622 12823 0.7 4.OOE.03 U5211. 1.2 NT Homo sapiens X28 region near ALD locus containing dual spedficity phosphatase 9 (DUSP9), ribosomal protein L18a (RPLI8a), Ca2+1Calmodulin-dependent protein kinase 1 (CAMKI), creatine transporter (CRTR), CDM protein (CDM), adrenoleukodystrophy protein > 2664 7752 12950 1,0 4.OOE-03 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 3487 8628 13739 0.9 4.OOE-03 AW188426,1 EST - HUMAN)d98f04.xl NCI--QGAF Col 8 Homo sapiens cDNA done IMAGE:2665279 3' 3487 8628 13740 0.9 4.OOE-03 AW188426.1 EST-HUMAN A98f04.xl NCI CGAP Col 8. Homo sapiens cDNA done IMAGE:2665279 3' 3572 8711 13810 0.8 4.OOE-03 Q13606 SWISSPROT OLFACTORY RECEPTOR 5M (OLFACTORY RECEPTOR-LIKE PROTEIN OLF1) 3836 8711 13810 0.8 4.OOE-03 Q13606 SWISSPROT OLFACTORY RECEPTOR 511 (OLFACTORY RECEPTOR-LIKE PROTEIN OLF1) 3852 8988 14088 0.9 4.0OE-03 AF060868.1 NT Mus musculus tumor susceptibility protein 101 (tsgIO1) gene, complete ods 3914 9048 1.8 4.OOE-03 AJ011 1712.1 NT Horno sapiens TNW1 gene, exons 1 - 11 (and joined CIDS) 4509 9628 14722 1.1 4.OOE-03 A1732754.1 EST-HUMAN abl 11a08.xS Stratagene lung (#937210) Homo sapiens cIDNA clone IMAGE:841142 X similar to contains Alu repetitive element; 4842 9949 15038 0.8 4.OOE-03 AL163301.2 NT Horno saRiens chromosome 21 segment HS21 Cl 01 5164 102 58 15328.13.2 4.OOE-03 J02187,1 NT Foot and mouth disease virus serotype A-12 11 gab capsid protein VP3 367 5545 10678 0.6 3.OOE-03 AF01 1920.1 NT Homo sapiens protein kinase CK2 catalytic subunit alpha gene, exon 1 861 6009 11170 2.6 3.00IE-03 AF01 1920.1 NT Horno sapiens protein kinase CK2 catalytic subunit alpha gene, exon 1 1458 6585 11759 0.8 3.OOE-03 A1133024.1 EST-HUMAN HA1611 Human fetal liver cDNA library Homo sapiens cDNA 1638 6764 11933 0,7 3.OOE-03 AA4681 10.1 EST-HUMAN nc73c05.sl NC1-WAP_Pr2 Homo saplens ciDNA done IMAGE:782984 similar to contains Alu repetitive element; 1 1707 6830 1.0 3.OOE-03 X87344.1 NT H.sapiens DMA, DMB, HILA21, IPP2, LMP2, TAP1, LMP7, TAP2, DOB, DQB2 and RING8, 9, 13 and 14 genes 2211 7319 0.9 3.OOE-03 AF055066.1 NT Homo saplens MI-IC class 1 Sion 2248 7354 1.4 3.OOE-03 Z32521.1 NT S.cereale (cv. Halo) mRNA for tdosephosphate Isomerase 2249 7355 12561 1.1 3.OOE-03 AJ271004.1 NT Mus musculus TFF3/ITF gene for Trefoil Factor 3/Intestinal Trefoil Factor protein, exons 1-3 2249 7355 12562 1.1 3.OOE-03 AJ271004.1 NT Mus musculus TFF3/ITF gene for Trefoil Factor 3/Intestinal Trefoil Factor protein, exons 1-3 2683 7770 12970 4.0 3.OOE-03 AF034863.1 NT Rattus norvegicus synaptic scaffolding molecule (S-SCAM) mRNA, complete cds 2963 8114 0.8 3.OOE-03 Y09006.1 NT Arabidopsis thallana rpoMt gene 3044 8195 13297 4.5 3.OOE-03 BE379296.1 EST-HUMAN 601237982F1 NIH_MGQ&_44 Homo sapiens cDNA clone IMAGE:3609933 5' 3112 8261 13365 2.6 3.OOE-03 AW802687.1 EST-HUMAN IL2-UM0076-240300-056- DO3 UM0076 Homo sapiens cDNA 3371 8514 13621 2.0 3.OOE-03 U34606.1 NT Mus musculus alpha-l(XVIII) collagen (COL18A1) gene, exon 1 and 2 3379 8522 6.8 3.OOE-03 Y12500.1 NT C.elegans samdc gene 3950 9083 14164 1.6 3.OOE-03 A1792278.1 EST - HUMAN ahO4f09.y5 Gessler Wilms tumor Homo sapiens cDNA done IMAGE: 1155689 5' 4058 9185 1.3 3.OOE-03 Z32521.1 NT S.cereale (cv. Halo) mRNA for tdoseehosphate isomerase L 14510 11.9 3.0 011432.1 NT Rattus norvegicus gdnf gene ___j--L9420 Page 58 of 196 4417 9537 14628 4.4 3.OOE-03 A1536141.1 EST-HUMAN xu8.P10.H3 conorm Homo saelens cIDNA 3' 4630 9744 14841 0,9 3.OOE-03 AL1 19067.1 EST HUMAN DKFZp761 B0712-rl 761 (synonym: hamy2) Homo sapiens cDNA done DKFZp761 B0712 5, 4727 9839 14931 1,5 3.OOE-03 A1732754.1 EST-HUMAN a1518a08.x5 Stratagene lung (#937210) Homo sapiens cIDNA done IMAGE:841142 Ysimilar to contains Alu repetitive element:

5089 10187 15265 1.1 3.OOE-03 4506414 NT Horno sapiens RAP1. GTPase activating protein 1 (RAP1GA1) mRNA 5089 10187 15266 1.1 3.OOE-03 4506414 NT Homo sapiens RAPI, GTPase activating protein 1 (RAP1GA1) mRNA 504 5668 10790 0.9 2.OOE-03 Q04652 SWISSPROT RING CANAL PROTEIN (KELCH PROTEIN) 504 5668 10791 0.9 2.OOE-03 Q04652 SWISSPROT RING CANAL PROTEIN (KELCH PROTEIN) 768 7899 2,4 2.OOE-03 T70874.1 EST HUMAN yd15h03xl Soares fetal liver spleen 1NFI.S Homo saplens cIDNA done IMAGE:108341 5' 1340 6468 11636 0.7 2.OOE-03 M20783.1 NT Human alpha-2-plasmin inhibitor gene, exons 6 and 7 1343 6471 11638 0.6 2.OOE-03 AA661605.1 EST-HUMAN nu86101.sl NCI CGAP Mv1 Homo sapiens cDNA done IMAGE: 1217593 1474 6601 11775 1.1 2.OOE-03 P48509 SWISSPROT PLATELET-ENDOTHELIAL TETRASPAN ANTIGEN 3 (PETA-3) (GP27) (MEMBRANE GLYCOPROTEIN SFA-1) (C13151 ANTIGEN) 1500 6626 11795 0.7 2.OOE-03 4557836 NT Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehiers Danlos syndrome type VI) (PLOD) mRNA 1500 6626 11796 0.7 2.OOE-03 4557836 NT Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehiers Danios syndrome type VI) (PLOD) mRNA 1573 6699 1.8 2.OOE-03 P29400 SWISSPROT COLLAGEN ALPHA 5(1V) CHAIN PRECURSOR 1733 6856 12034 1.4 2.OOE-03 AA450138.1 EST HUMAN zx42a10.rl Soares-total- fetus-Nb2W8-9w Homo sapiens cIDNA clone IMAGE:789114 5' 2203 7312 12524 1.0 2.OOE-03 A1-163302.2 NT Homo sapiens chromosome 21 segment HS21 Cl 02 2535 7631 1.7 2.0012-03 AW137782.1 EST HUMAN Ul-H-Bil-adi-g-10-0-Ul.sl NCI-CGAP-SuW Homo saplens cDNA done IMAGE:2717010 3' 3370 5' 3 13620 4.0 2.OOE-03 AA450138.1 EST-HUMAN zx42a10.rl Soares_totalfetus_Nb21-IFL9w Homo sapiens cDNA done IMAGE:789114 5' 8513 3615 81754 13847 4.9 2.0012-03 X87344.1 NT H.sapiens DMA, DIVIS, HILA21, IPP2, LIVIP2, TAP1, LMP7. TAP2, DOB, DQB2 and RING8, 9, 13 and 14 genes 3869 9005 14104 0.9 2.OOE-03 AB040802.1 NT Rattus norvegidus mRNA for SREB1, complete cds 4024 9152 14235 2.2 2.OOE-03 P03374 SWISSPROT ENV POLYPROTEIN [CONTAINS: COAT PROTEIN G1352; COAT PROTEIN GP361 4127 9253 9.8 2.OOE-03 U68491.1 NT Rattus norvegicus 5-hydroxytryptamine7 receptor gene, partial cds 4446 9566 14662 1.9 2.OOE-03 L42512.11 NT Drosop lia melanogaster shortsighted class 2 (shs) mRNA, complete cds 4446 9566 14663 1.9 2.OOE-03 L42512.1 NT Drosophila clanogaster shortsighted class 2 (shs) mRNA, complete cds 4608 9722 14817 0,9 2.OOE-03 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 4612 d9726 1.7 2.OOE-03 R87773.1 EST HUMAN yo45c02.sl Soares.adult brain N2b4HB55Y Homo sapiens cIDNA done IMAGE:180890 3' 5077 10175 0.8 2.OOE-03 AJ245167.1 NT Camelus dromedadus cvhpl g gene for immunoglobulin heavy chain variable region 386 5555 1.1 1.0OE-03 BE388904.1 EST HUMAN 601284694F1 N1H MGC 44 Homo saplens cDNA done IMAGE:3606670 5' 599 10730 1.4 1.OOE-03 H96471.11 H s cIDNA done IMAGE:232334 5' 99 EST UMAN yt9M8.rl SoarespineaLgiand_N3HPG Homo sapien I,.,... j Page 59 of 196 Table 4

2 812 5962 11119 0,8 1.OOE-03 A1720263.1 EST-HUMAN as70b08.xl Barstead colon HPI-RI37 Homo sapiens cDNA done tMAGE:2334039 3'shilar.

to TR:Q13825 013825 AU-BINDING PROTEINIENOYL-COA HYDRATASE.; 812 5962 11120 0,8 1.OOE-03 A1720263.1 EST-HUMAN as70b0B.xl Barstead colon HPI-RI37 Horno sapiens cDNA done IMAGE:2334039 Ysimilar to TR:01382d Q13825 AU-BINDING PROTEINIENOYL-COA HYDRATASE.; 1079 6217 11368 0,7 1.0OE-03 A1865788.1 EST-HUMAN M86a06.xl NCI CGAP Paril Homo saplens cDNA clone IMAGE:2422258 3, 1099 6237 11389 1.4 1.OOE-03 A1954572.1 EST-HUMAN wx93elO.xl NCI CGAP Me115 Homo saplens cDNA done IMAGE:2551242 3' 1150 6285 11438 1.5 1.0OE-03 A1692616.1 EST HUMAN wd86a01.xl NCI- CGAP_Lu24 Homo saplens cDNA clone IMAGE:2338440 Ysimilar to contains Alu repetitive element; 1982 7097 12300 1.3 1.OOE-03 P47808 SWISSPROT HIGH MOLECULAR WEIGHT FORM OF MYOSIN 1 (HMWMI) 2108 7219 12428 2.9 1.OOE-03 AJ131016.1 NT Homo sapi ns SCL gene locus 2951 8102 13208 1.2 1.OOE-03 A8033117.1 NT Homo saplens mRNA for K1AA1 291 protein, partial cds 3154 8303 13411 2,3 11.0OE-03 P18915 SWISSPROT CARBONIC ANHYDRASE VI PRECURSOR (CARBONATE DEHYDRATASE VI) (CA-VI) (SECRETED CARBONIC ANHYDRASE) (SALIVARY CARBONIC ANHYDRASE) 3154 8303 13412 2.3 1.OOE-03 P18915 SWISSPROT CARBONIC ANHYDRASE VI PRECURSOR (CARBONATE DEHYDRATASE VI) (CA-VI) (SECRETED CARBONIC ANHYDRASE) (SALIVARY CARBONIC ANHYDRASE) 3262 8409 13516 1.2 11.0OE-03 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 3496 8638 13750 0.8 1.0OE-03 U68061.1 NT Human MUC2 gene, promoter region 3496 8638 13761 0.8 1.0OE-03 U68061.1 NT Human MUC2 gene, promoter region 3855 8991 14091 1,0 1.OOE-03 Z49649.1 NT S.cerevisiae chromosome X reading frame ORF YJR1149w 4377 9499 14586 3.8 1.0OE-03 BE246536.1 EST-HUMAN TC13AP1 D4909 Pediatric pre-B cell acute lymphoblastc leukemia Baylor-HGSC project--TC13A Horno sapiens cDNA done TCBAP4909 4564 9682 14776 0.9 1.OOE-03 U29449.1 NT Caenorhabditis elegans spliced leader RNA (SL3 alpha), (SL4), and (SL5) genes 4717.9829 14920 1.8 1.0OE-03 A1073485.1 EST-HUMAN ov45c04.xl Scares tests NHT Horno saplens cDNA clone IMAGE: 1640262 3 4717 9829 14921 1.8 1.OOE-03 A1073485.1 EST - HUMAN ov45c04.xl Soares testis NHT Homo sapiens cDNA done IMAGE:1640262Y 4718 9830 6.2 1.0012-03 BE154067.1 EST HUMAN PMO-HT0339-200400-010-DO2 HT0339 Homo sapiens cIDNA 5009 10111 15181 17,0 1.OOE-03 046409 SWISSPROT APOLIPOPROTEIN A-IV PRECURSOR (APO-AIV) 1453 6580 11754 1.0 9.OOE-04 AW453046.1 EST-HUMAN Ul-H-BW1-ama-e-03-0-Ul. sl NCI-CGAP-Sub7 Homo sapiens cDNA clone IMAGE:3069388 3' 5131 10226 15301 0.8 9.OOE-04 L11910.1 NT Human rednoblastoma susceptibility gene exons 1-27, complete eds 1472 6599 1.4 8.OOE-04 X96469.1 NT X.laevis mRNA for C4SR rotein 3843 8979 14080 1,3 8.OOE-04 R07008.1 EST HUMAN y11211O.rl Soaresfetal liverspleen 1NFI-S Homosapiens cDNA done IMAGE:126691 6 4089 9215 4.2 8.OOE-04 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 4656 9768 14864 2,4 8.OOE-04 U29185.1 NT Homo, saplens prion protein (PrP) gene, complete cds 1786 6906 12094 0.6 7.OOE-04 L41825.1 NT Homo sapiens CYP17 gene, C end 2350 7452 12659 1.1 7.OOE-04 U29185.1 NT Horno saplens prion protein (PrP) gene, complete cds 2682 7769 12969 3.3 7.OOE-04 AL163210.2 NT Homo sapienschromosome 21 segment HS21C010 3243 8390 13497 1.0 7.OOE-04 4885170 NT Horno sapiens chromosome X open reading frame 6 (CXORF6) rnRNA 967 6112 11268 1.6 6.OOE-04 P07207 SWISSPROT NEUROGENIC LOCUS NOTCH PROTEIN PRECURSOR 967 6112 11269 1.6 6.0012-04 P07207 SWISSPROT NEUROGENIC LOCUS NOTCH PROTEIN PRECURSOR 6112 11270 1.6 6.0OE-04 P07207 SWISSPROT NEUROGENIC LOCUS NOTCH PROTEIN PRECURSOR Page 60 of 196 Table 4

* 3877 9013 14108 1.3 6.OOE-04 A1862525.1 EST HUMAN v415al 1.xl NCI CGAF_K1d12 Horno sapiens cDNA clone IMAGE:2402876 3' 3999 9130 14212 0.9 6.OOE-04 K011315.1 NT Homo saplens epsilon-1 pseudogene (IGHEP1) gene, W flanking region 3999 9130 14213 0.9 6.OOE-04 K01315.1 NT Homo sapiens epsilon-1 pseudogene (IGHEP1) gene, 5'flanking region 4092 9218 14301 3.3 6.OOE-04 u45983.1 NT Homo sapiens CCR8 chemokine receptor (CiVIKBR8) gene, complete cds 635 5794 10917 2.0 5.OOE-04 010341 SWISSPROT HYPOTHETICAL 29.3 KI) PROTEIN (ORF92) 1485 6612 0.7 5.OOE-04 AW851844.1 EST-HUMAN QVO-CTO225-021099-030-a07 CTO225 Horno sapiens cDNA 3367 8510 13617 1.4 5.OOE-04 AA548931.1 EST - HUMAN nk27ell.sl NCI---CGAP- --Col 1 Homo sapiens cDNA done IMAGE:1014764 Ysimilar to contains Alu repetitive element., 657 5814 10942 1.4 4.OOE-04 U32748.1 NT Haemophilus Influenzac Rd section 63 of 163 of the complete genome 829 5978 11138 0,5 4.OOE-04 A1720263.1 EST HUMAN as70b08.xl Barstead colon HPLRB7 Horno sapiens cDNA clone IMAGE:2334039 Y similar toTR.013825Q13825AU-BINDING PROTEINIENOYL-COAHYDRATASE.; 5978 11139 0.5 4.OOE-04 A1720263.1 EST HUMAN as70b08.xl Barstead colon HPI-RJ37 Homo sapiens cDNA clone IMAGE:2334039 Ysimilar to TR:013825 Q13825 AU-BINDING PROTEINIENOYL-COA HYDRATASE.; 1449 6576 11750 1.4 4.OOE-04 AW753356.1 EST-HUMAN RC3-CT0254-130100- 023401 CT0254 Homo sapiens cDNA 2037 7150 12355 0.6 4.OOE-04 AL163278.2 NT Horno saplens chromosome 21 segment H 1 7 2086 7198 1.2 4.OOE-04 AL046704.1 EST HUMAN DKFZp434D059j1 434 (synonym: htes3) Homo sapiens cDNA done DKFZp4341)059 5, 2586 7679 12880 1,0 4.OOE-04 096615 SWISSPROT SERICIN:25 (SILK GUM PROTEIN 2) 3002 8154 13254 0.8 4.OOE-04 P19474 SWISSPROT 52 KI) RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (RO(SS-A)I) 4228 9352 14432 2,8 4.OOE-04 AA576331.1 EST HUMAN nhlOalO.sl NCl-WAP-Col Homo sapiens cDNA done IMAGE:951930 Ysimilar to gb:M21121 T-CELL SPECIFIC RANTES PROTEIN PRECURSOR (HUMAN); 4228 9352 14433 2.8 4.OOE-04 AA576331.1 EST HUMAN nh10a10.sl NCI_PGAP- Coll Homo saplens cDNA. done IMAGE:951930 3' similar to gb:M21121 T-CELL SPECIFIC RANTES PROTEIN PRECURSOR (HUMAN); 4437 9558 14653 1.3 4.OOE-04 AA086324.1 EST HUMAN zn61 c08.sl Stratagene muscle 937209 Homo saplens cDNA done IMAGE:562670 X 5132 10227 15302 0.9 4.OOE-04 N483113.1 EST HUMAN yy78bl 0.sl Soares- mulUpleLsderosis-2NbHMSP Horno sapiens cDNA done IMAGE:279643 3'similar to contains Alu repetitive element; 5346 10474 1.0 3.0OE-04 AL1 19426.1 EST HUMAN DKFZp7611J221-rl 761 (synonym: hamy2) Homo sapiens cDNA done DKFZp761J221 5, 188 5382 10509 0.8 3.OOE-04 P49259 SWISSPROT 180 KG SECRETORY PHOSPHOLIPASE A2 RECEPTOR PRECURSOR (PLA2-R) 862 6010 11171 1.3 3.OOE-04 U83991.1 NT Human short chain acyl CoA dehydrogenase gene, 1 exons 1 and 2 1810 6929 1.2 3.OOE-04 A1399674.1 ESTHUMAN th23a02.xl NCI_WAP_Pr28 Homo sapiens cDNA done IMAGE:2119082 3, 3268 8414 13520 3.4 3.OOE-04 P25147 SWISSPROT INTERNALIN B PRECURSOR 3882 9018 14113 3.6 3.OOE-04 P49448 SWISSPROT GLUTAMATE DEHYDROGENASE 2 PRECURSOR (GDH) 39691, 9101 1,2 3.OOE-04 AJ271735.1 NT Horno sapiens n pseudoautosomal _region; segment 112 3991 9123 14207 1.1 3.0012-04 AF196779.1 NT Horno saplens transcrIptlon factor IGHM enhancer 3, JM1 1 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentlatIon-dependent protein, triple LIM domain protein 6, and synaptophysin genes, complete cds; and L-We calcium channel a> 4711 9823 4.5 3.OOE-04 BE153778.1 EST-HUMAN PMO-HT0339-190200-007-g12 HT0339 Homo saplens cIDNA 4784 9895 14988 0.9 3.OOE-04 AW937723.1 EST HUMAN QV3-DT0045-221299-046- d09 DT0045 Horno saplens cDNA 10152 1.3 3.OOE-04 1Y11204.1 NT V.carted gene encoding volvoxopsin 1 Page 61 of 196 Table 4

C p 169 5363 10489 1,2 2.OOE-04 AF217796.1 NT Homo sapiens SCG10 like-protein, helicase-like protein NHL, M68, and ADP-ribosyladon factor related protein 1 (ARFRP1) genes, complete cds 889 6037 11195 1.5 2.OOE-04 M86524.1 NT Human dystrophin gene 889 6037 11196 1.5 2.OOE-04 M86524.1 NT Human dystrophin gene 1162 6297 1.4 2.OOE-04 A1286021.1 EST HUMAN qh98el 1.xl Soares-NFL---T-WC- S1 Homo sapiens cIDNA done IMAGE:1855052 Ysimilar to contains MER312 MER3 repetitive element; 1169 6303 0.7 2.OOE-04 AL163203.2 NT Homo sapiens chromosome.21 segment HS21 C003 1791 6911 0.5 2.OOE-04 AF224268.1 NT Mus musculus T flanking region of PitK3 gene 2136 7246 1.3 2.OOE-04 AA478980.1 ESTHUMAN zu39b05.si Soares ovary turnor NbHOT Horno sapiens cDNA done IMAGE:740337 Ysimilar to contains Alu repetitive element; 2528 7624 12825 1.5 2.OOE-04 U66061.1 NT Human germline T-cell receptor beta chain TCRI3V17S1A1T, TCR13V2S1, TCIRSV10S1P, TCRI3V29S1P, TCIRW19S1P, TCIRW15S1, TCRI3V11S1A1T, HW relic, TCRBW8S1P, TCRI3V34S1, TCIRW14SI, TCIRBV3S1, TCIRBV4S1A1T, TRY4, TRY5, TRY6, TRY7, TRY8, TCIRB1)11, TCIRW1S1, TCIRW1S2,> 2958 8109 13215 1.0 2.OOE-04 A11124529.1 EST-HUMAN arn58c09.xl Johnston frontal cortex Homo saplens cDNA done IMAGE:1539760 3' 3389 8532 13637 2.5 2.OOE-04 RE082317.1 EST HUMAN OV2-BT0636-070500-194- b07 BT0636 Homo sapiens cDNA 3417 8559 13670 1,0 2.OOE-04 U34374.1 NT Human tyrosine kinase M (txk) gene, exons 9 and 10 3837 8973 14073 0.8 2.OOE-04 AW978441.1 EST-HUMAN EST390550 MAGE resequences, MAGP Homo saplens cDNA 4051 9178 4.6 2.OOE-04 U01029.1 NT Phaseolus vulgaris nitrate reductase (PVNIR2) gene, complete cds 4569 9687 14781 1.3 2.OOE-04 H96265.1 EST HUMAN yuOlel 1.rl Soares_pineal_gland N3HPG Homo saplens cIDNA done IMAGE:232556 5' 4569.9687 14782 1.3 2.OOE-04 H96265.1 EST HUMAN yuOlel 1.rl Soares_pineaLgiand N3HPG Homo sapiens cIDNA clone IMAGE:232556 5' 4689 9801 1.3 2.OOE-04 U09226.1 NT Gallus gallus proteasome 28 kDa subunit homolog MRNA, complete cds 4946 10051 15122 1.3 2.OOE-04 Q99297 SWISSPROT PUTATIVE MITOCHONDRIAL CARRIER YOR222W 4991 10094 15162 1.7 2.OOE-04 AB037997.1 NT Danio rerio hagoromo gene, exons 1 to 6, partial cds 750 5902 11048 1.0 1.OOE-04 H99646.1 EST-HUMAN yx2M9.al Soares melanocyte 2NbHM Homo sapiens cDirA clone IMAGE:262864 Ysimilar to contains Ll.tl L1 repetitive element:

1059 6198 11351 0.8 1.OOE-04 P1 1369 SWISSPROT RETROVIRUS-RELATED POL POLYPROTEIN [CONTAINS: REVERSE TRANSCRIPTASE ENDONUCLEASE] 1098 6236 11387 1.3 1.OOE-04 AW013847.1 EST-HUMAN Ul-H-BIO-aab-e-09-0-Ul. sl NCLWAP-Subl Homo sapiens cDNA done IMAGE:2708825 3' 1098 6236 11388 1.3 1.OOE-04 AW013847.1 EST HUMAN U]-H-BIO-aab-c-09-0-Ul. sl NCI_WAP-Subl Homo sapiens cDNA clone IMAGE:2708825 3' 1310 6438 1.0 1.OOE-04 U62918.1 NT Anguilla anguilla dopamine D1A1 receptor (d1A1) gene, complete cds 1610 6737 11906 1.2 1.OOE-04 AF148805.1 NT Kaposi's sarcoma-associated herpesvirus ORF 68 gene, partial cds; and ORF 69, kaposin, v FLIP, v-cydin. latent nuclear antigen, ORF K1 4, v-GPCR, putative phosphodbosylfonnyiglycinamidine synthase, and LAMP (LAMP) genes, complete cds 1610 6737 11907 1.2 1.OOE-04 AF148805.1 NT Kaposi's sarcoma-assodated herpesvirus ORF 68 gene, partial cds; and ORF 69, kaposin, v FLIP, v-cydin, latent nuclear antigen, ORF K14, v-GPCR, putative phosphoribosylformylgiycinamidine synthase, and LAMP (LAMP) genes, complete ods 7691 12895 1.3 1.OOE-04 263.1 EST_HUMAN riv95a02.sl NCI_PGAP Prl 6 Homo sapiens cIDNA done IMAGE:1 237514 similar to coniiins Alu repetitive element; 1 Page 62 of 196 Table 4

2600 7691 12896 1.3 1.0OE-04 AA714263.1 EST HUMAN nv95a02.sl NCI - CGAP Pr16 Homo sapiens cDNA clone IMAGE:1237514 similar to contains Alu repetitive element; 2655 7744 12943 1.2 1.OOE-04 BE218833.1 EST HUMAN hv45c08.xl NCI CGAP. Lu24 Horno sapiens cDNA done IMAGE:3176366 3' 2655 7744 12944 1.2 1.OOE-04 BE218833.1 EST HUMAN hv45c08.xl NCI CGAP Lu24 Homo sapiens cDNA done IMAGE:3176366 3, 3247 8394 13501 1.0 1.OOE-04 Q62203 SWISSPROT SPLICEOSOME ASSOCIATED PROTEIN 62 (SAP 62) (SPLICING FACTOR 3A SUBUNIT 2) (SF3A66) 3675 8814 13908 1.1 1.OOE-04 A1440282.1 EST-HUMAN q01fl 1.xl NCIWAP-Gas4 Homo sapiens cDNA done IMAGE:2140269 3'similar to contains Alu repetitive element; 3971 9103 14186 1.8 1.OOE-04 M14042.11 NT Mouse alpha 1 type-IV collagen mRNA 4362 9484 14575 0.9 1.0OE-04 P08547 SWISSPROt LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 4665 9777 14874 3.0 1.OOE-04 015117 SWISSPROT FYN-BINDING PROTEIN (SI-P- 76 ASSOCIATED PROTEIN) (SLAP-1 30) 5028 10129 15203 1.6 1.OOE-04 7662015 NT Homo sapiens KIAA0237 gene product (K1AA0237), mRNA 5028 10129 15204 1.6 1.OOE-04 7662015 NT Homo sapiens KIAA0237 gene product (K1AA0237), mRNA 5033 10133 15208 1.0 1.OOE-04 A1357156.1 EST-HUMAN qx62h04.xl NCI_PGAP_GC4 Horno sapiens cDNA done IMAGE:2005975 3' 5148 10243 0.9 1.0012-04 Z72560.1 NT S.cerevisiae chromosome VII reading frame ORF YGI-038c 683 5838 10971 0.6 9.OOE-05 AA7118933.1 ESTHUMAN ah45cl 1.sl Soares testis NHT Homo sapiens cDNA done 1292468 3' 1589 6715 11885 1.1 9.OOE-05 AL04381 0.1 EST HUMAN DKFZp434A0128-rl 434 (synonym: htes3) Homo sapiens cDNA done DKFZp434A0128 C 3995 9127 14210 0.8 9.OOE-05 A1762209.1 ESTHUMAN wi54cl 1.xl NCI_PGAF_Col 6 Homo sapiens cDNA clone IMAGE:2394068 Ysimilar to contains MER6.1:1 MER6 repetitive element; 804 5955 11108 1.5 8.OOE-05 AJ251646.1 NT Pisurn sativurn mRNA for beta-1, 3 glucanase (gns2 gene) 846 5995 1.2 8.OOE-05 AJ251646.1 NT Pisum sativurn mRNA for Peta-1,3 glucanase (gns2 gene) 2920 8071 0.8 8.00IE-05 M83575.1 NT Human platelet-derIved growth factor A chain (PDGFA) gene, exons only 4852 9959 1.2 8.OOE-05 F281172.1 EST-HUMAN HSPD16734 HM3 Homo saplens cDNA done s3000003H04 344 5525 10650 1.0 7.OOE-05 AW847445.1 EST HUMAN RC3-CT0208-220999-011 1 - E04 CTO208 Homo sapiens cDNA 344 5525 10651 1.0 7.OOE-05 AW847445.1 EST-HUMAN RC3-CTO208-220999-01 11- E04 CT0208 Homo sapiens cDNA 555 5718 10834 1.1 7.OOE-05 L49075.1 ES-LHUMAN HUM072014F Human fovea c5NA Horno sapiens cDNA done EST HFD072014 555 5718 10835 1.1 7.OOE-05 L49075.1 EST-HUMAN HUM072014F Human fovea eDNA Homo sapiens cDNA done EST HFD072014 1039 6179 11332 0.8 7.OOE-05 Q22949 SWISSPROT PROBABLE GLYCEROL-3- PHOSPHATE ACYLTRANSFERASE, MITOCHONDRIAL PRECURSOR(WAT) 1083 6221 11372 0.7 7.OOE-05 TO5902.1 EST HUMAN EST03791 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA done HFBD136 2686 7773 12972 1,9 7.OOE-05 AL163278.2 NT Horno sapiens chromosome 21 segment HS2 1 1C078 3121 8270 13375 5.8 7.OOE-05 A13009080.11 NT Dictyostellurn discoldeum gene for TRFA, complete cds 3475 8616 13728 1.1 7.OOE-05 AL163303.2 NT Homo sapiens chromosome 21 segment HS21C1 03 4276 9401 14485 1.3 7.OOE-05 A1.163201.2 NT Homo sapiens chromosome 21 segment HS21 C001 4344 9467 14555 0.8 7.OOE-05 U60980.1 NT Caenorhabdifis elegans Skplp homolog mRNA, complete cds 5202 10293 1.1 7.OOE-05 AA367612.11 EST-HUMAN EST78713 Placenta 1 Homo sapiens cDNA 1981 7096 12298 0.6 6.OOE-05 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 1981 7096 12299 0.6 6.OOE-05 4885170 NT Horno sapiens chromosome X open reading frame 6 (CXORF6) mRNA 0,5 6.OOE-05 A1655241.1 EST HUMAN wb54h06.xl NCI - CGAP-GC6 Homo sapiens cDNA clone IMAGE:2309531 3' similar to qb:J03250 DNA TOPOISOMERASE 1 (HUMAN); Page 63 of 196 Table 4

1 kh C 2652 7741 12941 0.9 6.OOE-05 Z84506.1 NT H.sapiens flow-sorted chromosome 6 Hindill fragment. W6gA28B10 2652 7741 12942 0.9 6.OOE-05 Z84506.1 NT H.sapiens flow-sorted chromosome 6 Hindill fragment, SC6pA22131310 2783 5820 10947 0.8 6.OOE-05 AF053630.1 NT Homo saplens monocytelneutrophil elasta c Inhibitor gene, complete ods 1382 6509 11673 5.2 5.OOE-05 AW392086.1 EST HUMAN QV4-ST0234-241199-040- hl 1 ST0234 Homo sapiens cIDNA membrane protein-like (LOC55895), mIRNA 1819 6938 13082 0,6 5.OOE-05 8923891 NT Homosaplens 22kDa peroxisomal 2831 7982 0.9 5.OOE-05 AJ251058.1 NT Homo sapiens MEP1A gene, promoter region nd exon 1 3897 9032 14125 3.0 5.OOE-05 AJ251884.1 NT Homo sapiens partial SLC22A3 gene for extraneuronal monoamine transporter (EMT), exo. n 1 2220 7327 1.4 4.OOE-05 AJ289880.11 NT Homo sapiens KIAA0851 ge (partial), XT3 gene and LZTFL1 gene 2776 5415 14834 1.6 4.OOE-05 U12821.1 NT Human renin (REN) gene, V flanking region 4624 9738 7.1 4.OOE-05 AL163280.2 NT Horno sapiens chromosome 21 segment HS21 C080 NFI-S-S1 Homo saplens cDNA done qh64cl 0.xl Scares_fetal-liver_spleen-1 IMAGE:1849458 3'sImilar to contains Alu repetitive element;contains element KER repetitive 665 5821 10949 0.8 3.OOE-05 A1248061.1 EST HUMAN element; SI Homo sapiens cIDNA done IMAGE:2814100 3' 1043 6183 11336 1.4 3.OOE-05 AW273851.1 EST HUMAN xv24g03.xl Soares NFI- JjG13C 2688 7775 12974 0,9 3.OOE-05 Q62234 SWISSPROT SKELEMIN 4285 9410 14496 6.9 3.OOE-05 BE169211.1 EST HUMAN PM1-HT0521 120200-001- el 0 HT0521 Homo sapiens cIDNA 4285 9410 14497 6.9 3.OOE-05 BE1169211.1 EST HUMAN PM1-HT0521-120200-001- elo HT0521 Homo sapiens cDNA 4365 9487 14577 1.2 3.0012-05 AA366679.1 EST HUMAN EST79996 Placenta 1 Homo saplens cIDNA similar to similar to R53-associated protein cIDNA similar to similar to p53-assodated protein Homo 4365 9487 14578 1,2 3.OOE-05 AA368679.1 EST HUMAN EST79996 Placenta 1 sapiens 4519 9638 14729 0.9 3.OOE-05 AF149773.1 NT Homo sapiens NOD1 prote (NOD1) gene, exons 1, 2, and 3 NFI-S-S1 Homo sapiens cIDNA clone qh64cl 0.xl Soares-fetai_iiver_spleen-1 IMAGE:1849458 Ysimilar to contains AW repetitive element;contains element KER repefitive 4731 5821 10949 0.8 3.OOE-05 A1248061.1 EST HUMAN element; Honno sapiens cDNA clone IMAGE:1855052 3' similar qh9Bel 1.xl Soareg_NFL-T-GBC_Sl 2276 7382 12590 0.6 2.OOE-05 A1286021.1 EST_HUMAN to contains MER312 MER3 repetitive element; 2538 7634 12834 1.1 2.OOE-05 M13792.1 NT Human adenosine deaminase (ADA) gene, complete cds 2681 7768 13353 2.6 2.OOE-05 AA160562.1 EST HUMAN zq46a12xl Stratagene hNT neuron (#937233) Homo saplens cIDNA clone IMAGE:632734 5' 3098 8249 13559 1.5 2.OOE-05 BE066036.1 ESTHUMAN similar to contains AJu 1 repetitive element;contains element L1 repeutive element; 3304 8449 0.9 2.OOE-05 AF1 84614.1 NT IRC3-BT0319-120200-014-h08 BT031 9 Homo sapiens cIDNA Homo sapiens p47-phox (NCF1) gene, complete cds 3323 8467 13582 1.1 2.OOE-05 X8921 1.1 NT H.sapie NA for end29enous retroviral like element 3358 8501 13610 5.3 2.OOE-05 AL.163207.2 NT Homo sapiens chromosome 21 segment HS21C007 left of chromosome XV 3449 8591 1.0 2.OOE-05 X9WS.1 NT S.cerevisiae 12,8 Kbp fragment of the 3737 8874 0.9 2.OOE-05 AL039107.1 EST HUMAN arm DKFZp5661064_rl 566 (synonym: M2) Homo saplens cDNA clone DMp5661064 5' qql 3a08.xl Soares_NhHIVIPti-S1 Homo saplens cDNA done IMAGE:1 932374 Ysimilar to 4510 9629 14723 1.0 2.OOE-05 A1263349.1 EST - HUMAN contains MER18.153 MER18 repetitive element; 4592 9706 1.1 2.OOE-05 BE378471.1 EST HUMAN 601236455F1 Ni,MGC 44 Homo saplens cDNA clone IMAGE:3608653 5' 4782 9892 14985 0.8 2.00 131016.1 NT Homo saplens SCL gene lows OCIE-05 AL.163282.2 NT Homo sapiens chromosome 21 segment HS21C082 Page 64 of 196 Table 4 n 1,n 3238 8385 13493 0.9 1.OOE-05 AA631 101.1 ESTHUMAN nq77g06.sl NCI CGAP Pr22 Homo sapiens cDNA done IMAGE:1 158394 3' 3599 8738 13833 1.7 1.OOE-05 AF088273.1 NT Drosophila melanogaster strain Larrito 120 Suppressor of Hairless (Su(H)) gene, partial cds 3886 9022 14115 9,2 1.OOE-05 P81274 SWISSPROT MOSAIC PROTEIN LGN 4082 9209 14293 1.1 1.OOE-05 AL163203.2 NT Homo sapiens chromosome 21 segment HS21C003 4180 9306 14385 2.0 1.OOE-05 AA431 119.1 EST HUMAN zw69g04xl Soares_testis NHT Homo sapiens cDNA done IMAGE:781494 5' 4742 9853 14945 2.2 1.OOE-05 AW419134.1 EST-HUMAN xAgol 1.xl NCI CGAP Lu34.1 Horno sapiens cDNA clone IMAGE:2856548 3' 4882 9990 15071 4.5 1.OOE-05 AL163246.2 NT Homo sapiens chromosome 21 segment HS21 C046 2637 7726 12930 2.4 9.0012-06 A158381 1,1 EST HUMAN tt73a06.xl NCI_WAP HSC3 Homo sapiens cIDNA done IMAGE:2246386 3' 3057 8208 13307 3.7 9.OOE-06 A1218983.1 EST-HUMAN -- qgl 1 b08.xl Soares_placenta-8togweeks-2NbHP8to9W Homo sapiens cDNA done IMAGE:1759191 3' 3559 8698 2.9 9.OOE-06 M61755.1 NT Human alanine:giyoxylate aminotransferase (AGXT) gene, exons 1 and 2 2483 7944 12783 0.6 8.OOE-06 AW362539.1 EST HUMAN IRC3-CT0283-201 1 W01 1- h11 1 CT0283 Homo sapiens cDNA 963 6108 0.6 7.OOE-06 AA669729.1 ESTHUMAN abgOfIO.sl Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE:854251 Ysimilar to contains MER20M MER20 repetitive element; 1420 6548 11723 1.2 7.0OE-06 7662177 NT Homo sapiens KIAA0555 gene product (K1AA0555), mRNA 2844 7995 6.7 7.0012-06 A1368252.1 EST HUMAN qw16g09.xl NCI - CGAP_Ut3 Horno sapiens cDNA done IMAGE:1991296 3' similar to contains Alu repetitive element; 3516 8657 0.9 7.OOE-06 AA385542.1 EST-HUMAN EST99205 Thyroid Homo sapiens cDNA 5' end similar to EST containing L1 repeat 2887 80M 13139 1.1 6.OOE-06 BE069189.1 EST HUMAN QV3-BT0379-010300-105-dl 1 BT0379 Homo sapiens cDNA 3635 8774 13868 1.0 6.OOE-06 BE069189,1 EST HUMAN. QV3-BT0379-010300-105- dl 1 BT0379 Homo.sapiens cDNA 4647 8062 13169 1.8 6.OOE-06 Q01456 SWISSPROT OVARIAN ABUNDANT MESSAGE PROTEIN ( M PROTEIN) 4653 9765 14860 2.0 6.OOE-06 A1040099.1 EST-HUMAN oxO8eO2.xl Soares--- fetaUiver - spleen-1 NFL.SS1 Homo sapiens cDNA clone IMAGE:1655738 3' similar to contains MER8.2 MER8 repetitive element; 631 5790 10912 1.5 4.OOE-06 R1 6267.1 EST HUMAN ya48c03xl Scares Infant brain I NIB Homo sapiens cDNA done IMAGE:53254 5'similar to contains Alu repetitive element;contains L1 repetitive element; 828 5977 11137 3.2 4.OOE-06 AW103354.1 EST-HUMAN xc69g12.xl NCI-MAP-Eso2 Homo sapiens cDNA done IMAGE:2589574 Ysimilar to contains Alu repetitive element;contalns element MER21 repetitive element; 1312 6440 11606 1.8 4.OOE-06 A1334928.1 EST HUMAN th33e09.xl Ncl_pGAP HSC2 Homo sapiens cDNA done iMAGE:2056168 3 1312 6440 11607 1.8 4.OOE-06 A1334928.1 EST HUMAN th33c09.xl NCI_WAp_lISC2 Homo sapiens cDNA done IMAGE:2056168 3' 1329 6458 2.4 4.0012-06 X87344.1 NT H.sapiens DMA, DIVIB, HILA-Z1, IPP2, LIVIP2, TAP1, LIVIP7, TAP2, DOB, DQB2 and RING8, 9, 13 and 14 genes 2218 7326 12540 1.2 4.OOE-06 AW015401.1 EST-HUMAN Ul-H-BIO-aat-f-05-0-Ul. sl NCI-CGAP_Subl Homo sapiens cDNA done IMAGE:2710425 X 3025 8177 13278 1.4 4.OOE-06 AF198349.1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete ods 3500 8642 0.9 4.OOE-06 H92947.1 ESTHUMAN yt94cl 1.sl Soares_pineal--- glano_N31-1PG Homo sapiens cDNA done IMAGE:231956 3' similar to contains LOR1 repetitive element; 3819 8956 14052 1.8 4.OOE-06 AW84W95.1 EST HUMAN IL3-CTO214-150200-074- BO3 CT0214 Horno sapiens cDNA 4702 9814 14910 1.6 4.OOE-06 A1886939.1 EST-HUMAN W94cl 0.xl NCLWAP-13m25 Horno sapiens cDNA clone. IMAGE:2432562 3'sImilar to contains element MER22 repetitive element; 9947 150361 1.0 4.OOE-06 AL163279.2 NT Horno saplens chromosome 21 segment HS21C079 Page 65 of 196 Table 4 tal A32C53 z134b08.sl.Soares fe ljiver-spicerLl NFLS-SI Horno sapiens cUNA clone IMAGE.

2117 7228 12436 1.3 3.OOE-06 AA700562.1 EST HUMAN 3' similar to contains L1A1 L1 repetitive clement.

S1 Homo sapiens cDNA done IMAGE:432663 zJ34b08.sl Soare:-fetaLlivef-spleerLINFLS - 2117 7228 12437 1.3 3.0012-06 AA700562,1 EST-HUMAN 3' similar to contains 1-1.0 1.1 repetitive element; Homo saplens cDNA done IMAGE:1409252 Ysimilar to ak48gl I.sl Soares---testis_NI-IT 2891 8042 13142 1.0 3.OOE-06 AA868218.1 EST HUMAN contains LTRIA3 LTR1 repetitive element, cuNA done IMAGE:2425616 Ysimilar to Homo 3228 8376 2.1 3.0012-06 A1857779.1 EST HUMAN W22a05.xl NCLWAP-Utl sapiens TR:060734 060734 LINE-1 LIKE PROTEIN;contains Ll.t2 L1 repetitive element; HN13 Homo sapians cIDNA clone IMAGE:3124151 3 3716 8853 13944 1.3 3.OOE-06 BE047094.1 EST HUMAN hq64d112.xl NCI CGAP HN13 Horno sapiens cDNA clone IMAGE:3124151 3 3716 8863 13945 1.3 3.0011-06 BE047094.1 EST HUMAN hq64d12.xl NCI CGAP Homo sapiens cDNA clone IMAGE:77275 V similar yb78b1O.rl Stratagene ovary (#937217) 4376 9498 14585 0.9 3.OOE-06 T50266.1 EST HUMAN to contains L1 repetitive element exons 1-5 (encoding alpha-l- Homo sapiens gene for alpha-1-microglobulin-bikunin, 4461 9581 14678 4.3 3.OOE-06 X54816.1 NT microglobulin, N-terminus.) 5389 1.1 2.OOE-06 P54366 SWISSPROT HOMEOBOX PROTEIN GOOSECOID PROTEASE; REVERSE TRANSCRIPTASE., POL POLYPROTEIN [CONTAINS:

1550 6677 2.3 2.OOE-06 P21414 SWISSPROT ENDONUCLEASE] 1 Horno sapiens cIDNA done IMAGE:2297068 Ysimilar to 2326 7429 12638 1.1 2.OOE-06 A1672138.1 EST-HUMAN waO4a03.xl NCLWAP_Kidl 2418 7517 12724 0.8 2.OOE-06 P04929 SWISSPROT contains MER3011 MER30 repetitive element; 2519 7615 12817 2.7 2.OOE-06 P0671 9 SWISSPROT HISTIDINE-RICH GLYCOPROTEIN PRECURSOR KNOB-ASSOCIATED HISTIDINE-RICH PROTEIN PRECURSUR (KAHRP) ovarlan (#937219) Horno sapiens cDNA done IMAGE:595232 cancer zpO2e05.rl Stratagene 3700 8839 13930 1.5 2.OOE-06 AA173518.1 EST HUMAN 5' 3710 8848 13938 1.5 2.OOE-06 AB030896.1 NT Mus musculus gene for odorant receptor A16, complete cels TRANSPORTER 2 (SOLUTE CARRIER FAMILY 22, 31 5240 10345 0.7 1.OOE-06 076082 SWISSPROT ORGANIC CATION/CARNITINE MEMBER 5) (HIGH-AFFINITY SODIUM-DEPENDENT CANITINE COTRANSPORTER) D6MM5E (D6MmSe) mRNA, comp!ete cds 641 5800 10926 0.6 1.0OE-06 AF084364.1 NT Mus musculus 1041 6181 1.0 1.0OE-06 A1206097.11 EST-HUMAN protein qg28b09.xl NCI_MAP_KI0 Horno sapiens cDNA done IMAGE:1762361 Ysimilar to TR:Q13538 Q13538 ORF2: FUNCTION UNKNOWN.; 1435 6562 11738 0.8 1.0OE-06 P09125 SWISSPROT MEROZOITE SURFACE PROTEIN CMZ-8 1507 6634 11803 1.1 1.OOE-06 AL163278.2 NT Homo sapiens chromosome 21 segment H521 C078 Soares_fetal-liver-spleen_1NFLS-S1 Homo saplens cDNA clone IMAGE:429982 zi06a12.sl 1554 6681 11B49 0.9 1.0OE-06 AA034141.1 EST-HUMAN 3 similar to contains AAu repetitive elemen!., WLS-S1 Homo sapiens cDNA clone IMAGE:429982 zj06a12.sl SoaresJetal - liver_spleerLl 1554 6681 11850 0.9 1.OOE-06 AA034141.1 EST HUMAN Ysimbar to contains Alu repetitive element; RNA POLYMERASE III LARGEST SUBUNIT 1565 6692 12261 -1,2 1.OOE-06 P27625 SWISSPROT DNA-DIRECTED 1949 7066 12262 1.7 1.0OE-06 AF184614.1 NT Homo saplens p47-phox (NCF1) gene, complete ods 1949 7 E6-6 1.7 1.OOE-0 AF184614.1 NT Horno sapiens p4T-Rhox (NCF1) gene, complete ods I b and Intron 11 b, and putative M8604 Met protein (M8604 Met) Human ABL gene, exon 4273 9398 14481 11,1 1.OOE-06 U07561.1 NT lgene, complete ods 10143 15220 1,4 1.OOE-06 285.2 Homo sapiens chromosome 21 segment HS21C085 Page 66 of 196 Table 4 fd- 5043 10143 15221 1,4 1.OOE-06 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 357 5536 10666 0.5 9.OOE-07 AF003529.1 NT Homo sapiens glypican 3 (GPC3) gene, partial cds and flanking repeat regions 357 5536 10667 0.5 9.OOE-07 AF003529.1 NT Homo sapiens glypican 3 (GPC3) gene, partial ods and flanking repeat regions 1034 6174 0.9 8.OOE-07 BE047871.1 ESTHUMAN tz43d06s1 NCI CGAP Bm52 Homo sapiens cDNA done IMAGE:2291339 5, 3498 8640 1.4 8.OOE-07 BE047871.1 F-ST HUMAN tz43d06.yl NCI CGAP 13m52 Homo sapiens cDNA done IMAGE:2291339 5' 4661 9773 14868 4.5 8.OOE-07 A1288596.1 EST'HUMAN q182907.xl Soares_NhiIMPu S1 Homo sapiens cDNA done IMAGE:1878876 X 4661 9773 14869 4.5 8.OOE-07 A1288596.1 EST HUMAN g182g07.xl Soares_Nhi- IMPu_ 1 Homo sapiens cDNA done IMAGE:1878876 3, 1822 6941 12131 0.9 7.OOE-07 AF167341.1 NT Homosapiens membrane intedeukin 1 receptor accessory protein (11-1 RAP) gene, exons 10 and 11 1870 6988 12173 0.9 6.OOE-07 AW855558.1 EST HUMAN CM3-CTO277-221099-024- el 1 CT0277 Homo sapiens W 2442 -7541 12749 0.9 6.OOE-07 AF019413.1 NT Homo sapiens HLA class Ill region containing tenascin X (tenascin-X) gene, partial cds; cytochrome P450 21-hydroxylase (CYP2113), complement component C4 (C4B) G1 1.

helicase (SK12W), RD, complement factor 8 (13% and complement component C2 (C2) genes,> 3891 9027 1.7 6.OOE-07 P41479 SWISSPROT HYPOTHETICAL 24.1 KI) PROTEIN IN LEF4-P33 INTERGENIC REGION 323 5506 1.2 5.OOE-07 A1831893.1 EST-HUMAN wh601 0.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:2385547 3' 2995 8147 0.9 5.OOE-07 A1831893.1 EST-HUMAN wh6010.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:2385547 3' 4547 9666 14755 1.4 5.OOE-07 AF149774.1 NT Homo sapiens NODI protein (NOD1) gene, exons 4 through 14 and complete cds 3918 9052 14140 2.0 4.OOE-07 AW009602.1 EST-HUMAN ws84h05.xl NCI CGAP Co3 Homo saplens cIDNA done IMAGE:2504697 3' 434 5601 10732 1.7 3.OOE-07 U19719.1 NT Human microfibrikassociated glycoproteln (MFAP2) gene, putative promoter region and alternatively spliced untranslated exons 568 5729 10846 0.8 3.OOE-07 A.1271735.1 NT Homo saplens Xcl pseudoautosomal region; segment 112 1352 6480 11646 0.8 3.OOE-07 M99149.1 NT Human polymorphic microsatellite DNA 1608 6735 0.8 3.OOE-07 M64857.1 NT Human lgK subgroup 1 germline gene, exons 1 and 2, V-region 018 allele 2000 7115 1.0 3.OOE-07 AA526763.1 EST-HUMAN ni56b09.si NCLWAP-Ov2 Homo sapiens cDNA done IMAGE:980825 similar to contains Alu repetitive element;contalns L1A3 L1 repetitive element; 2242 7348 12558 1.2 3.OOE-07 M99149.1 NT Human polymorphic microsatellite DNA 2420 7519 12726 6.2 3.OOE-07 BE0OW77.1 EST-HUMAN MRO-BN01 15-020300- 001411 BN01 15 Homo saplens cDNA 2420 7519 12727 6.2 3.OOE-07 BE005077.1 EST-HUMAN MRO-BN01 15-020300-001- fl 1 BN01 16 Honno sapiens eDNA 2998 8150 13250 0.9 3.OOE-07 T84704.1 EST HUMAN yd50f12.rl Soaresfetal liverspleen 1NIFI-S Homo sapiens cDNA done IMAGE:1116955' 3122 8271 13376 1.8 3.00IE-07 P38739 SWISSPROT HYPOTHETICAL 63.8 KD PROTEIN IN GUTI-RIM1 INTERGENIC REGION PRECURSOR 4195 9321 14406 2.4 3.OOE-07 AF044206.11 NT Homo sapiens cydooxygenase (COX-2) gene, promoter and exon 1 4195 9321 14407 2.4 3.OOE-07 AF044206.1 NT Homo sapiens cyclooxygenase (COX-2) gene, promoter and exon 1 4364 9486 14576 0.9 3.OOE-07 Q10364 SWISSPROT PUTATIVE SERINEfri-IREONINE- PROTEIN KINASE C22E12.14C 4654 9766 14861 0.9 3.OOE-07 A1797236.1 EST HUMAN we86bl 2.xl Soares NFL T GBC S1 Honno saplens cDNA done IMAGE:2347967 3 4996 10099 15166 1.5 3.OOE-07 T57850.1 EST-HUMAN yo14h09.sl Stratagene lung (#937210) Honno sapiens cDNA clone IMAGE:80705 3' similar to similar to gb:M62982 ARACHIDONATE 12-LIPOXYGENASE (HUMAN) 4996 10099 15167 1.5 3.OOE-07 T57850.1 EST-HUMAN yc14h09.al Stratagene lung (#937210) Homo sapiens cDNA done IMAGE:80705 Wsimilar to similar to gb:M62982 ARACHIDONATE 12-LIPOXYGENASE (HUMAN) ", 5235 10339 0.9 2.OOE-07 AF262988.1 NT Homo sapiens TRF2-Interacting telomeric RAPI protein (RAP1) mRNA, complete cds 1 Page 67 of 196 Table 4

148 5344 10472 1.6 2.OOE-07 L77569.1 NT Horno sapiens DiGeorge syndrome critical region, telorneric end 148 5344 10473 1.6 2.OOE-07 L77569.1 NT Homo saplens DiGeorge syndrome critical region. telomeric end 5368 10494 40.0 2.OOE-07 U38849.1 NT Fugu rubripes beta- cytoplasmic(vascular) actin gene, complete cds Homo sapiens homeobox protein CDX4 (C1DX4) gene, complete cds and flanking repeat 731 5885 11027 0.6 2.0012-07 AF003530.1 NT regions Horno, sapiens homcobox protein CDX4 (CDX4) gene, complete cds and flanking repeat 731 5885 11028 0.6 2.OOE-07 AF003530.1 NT regions zrO8b07.si Stratagene NT2 neuronal precursor 937230 Homc, sapiens cDNA done IMAGE:650869 Ysimilar to gb131860 GLYCOPHORIN A PRECURSOR (HUMAN); contains 928 6075 11233 1.1 2.OOE-07- AA223260.1 EST HUMAN Alu repetitive element; yc15g04.sl Stratagene lung (#937210) Homo sapiens cDNA done IMAGE:80790 X similar to 929 6076 11234 1.2 2.OOE-07 T63042.1 EST HUMAN contains Ll repetitive element 1147 6282 11435 1.3 2.OOE-07 Q26768 SWISSPROT 116 AUTOANTIGEN 1582 6708 11879 0.7 2.OOE-07 Q09701 SWISSPROT HYPOTHETICAL 72.5 KI) PROTEIN C2F7.10 IN CHROMOSOME 1 3633 8772 13866 14.7 2.OOE-07 AF125348.1 NT Homo sapiens caveolin 1 (CAV1) gene, exon 3 and partial cds xaO5hO7.xl Soares_NFL-T-WC-S1 Homo sapiens cDNA done IMAGE:2567485 Ysimilar 5078 10176 __15255 1.0 2.OOE-07 AW070995.1 EST HUMAN to WP:C38H2.1 CE00923 PROBABLE RAI3GAP DOMAINS; xaO5h07.xl Scares NFL - T - GBC SI Homo sapiens cDNA done IMAGE:2567485 3' similar 5078 10176 15256 1.0 2.OOE-07 AW070995.1 EST-HUMAN to W13:C381-12.11 CE00923 PROB,BLE RAI3GAP DOMAINS; 5198 10289 15358 1.0 2.OOE-07 AL163301.2 NT Homo sapiens chromosome 21 segment HS21 Cl 01 1086 6224 1.2 1.OOE-07 AL163282.2 NT Homo sapiens chromosome 21 segment HS21 C082 2331 7434 12642 0.9 11.0OE-07 P10263 SWISSPROT RETROVIRUS-RELATED GAG POLYPROTEIN (VERSION 1) Homo sapiens RAS, member of RAS oncogene family-like 2A (RABL2A), transcript variant 2, 2403 7502 12709 0.9 1.OOE-07 7549818 NT mRNA 2797 6632 11801 0.6 1.OOE-07 P09256 SWISSPROT GLYCOPROTEIN GPV 3684 6224 1.311.0OE-07 AL163282.2 NT Homo sapiens chromosome 21 segment HS21 C082 591 7893 0.9 8.OOE-08 A191 1352.1 EST HUMAN wdl 6b05.xl Soares_NFLGBC_Sl Homo sapiens d3NA done IMAGE:2328273 3' 73 5279 10406 1.1 7.0012-08 Q02357 SWISSPROT ANKYRIN 1 (ERYTHROCYTE ANKYRIN) 1338 6466 11634 4.6 7.OOE-08 X04809.1 NT Rat mRNA for dbosomal protein L31 3528 8669 13772 1.1 7.OOE-08 P15305 SWISSPROT DYNEIN HEAVY CHAIN (DYHC) 3528 8669 13773 1.1 7.OOE-08 P15305 SWISSPROT DYNEIN HEAVY CHAIN (DYHC) 800 5951 11101 1.0 6.OOE-08 AL163248.2 NT Homo sapiens chromosome 21 segment HS21 C048 800 5951 11102 1.0 6.OOE-08 AL1163248.2 NT Homo sapie s chromosome 21 segment HS21C048 2312 7415 12624 0.6 6.OOE-08 BE144398.1 EST HUMAN MRO-HT0166-191199-004g09 HT0166 Homo sapiens cDNA 4155 9281 14361 1.0 6.OOE-08 AL163248.2 NT Homo saplens chromosome 21 segment HS21C048 79 5285 10412 1.0 5.OOE-08 ALl 63303.2 NT Homo sapt ns chromosome 21 segment HS21 Cl 03 nh03b09.sl NCI WAP_Thyl Homo sapiens cDNA done IMAGE:943193 similar to contains 2189 7298 12513 0.7 5.OOE-08 AA493851.1 EST HUMAN Alu repetitive element; 5178 10270 15341 1.1 5.OOE-08 Q06278 SWISSPROT ALDEHYDE OXIDASE 1724 6847 12025 1.1 4.0012-08 P25723 SWISSPROT DORSAL-VENTRAL PATTERNING TOLLOID PROTEIN PRECURSOR 17 6847 12026 1.1 4.OOE-08 P25723 SWISSPROT ENTRAL PATTERNING TOLLOID PROTEIN PRECURSOR Page 68 of 196 s- kf 5184 10276 15344 7.2 3.OOE-08 AA191195.1 EST HUMAN zq45d05.M Stratagene hNT neuron (#937233) Homo saplens cDNA done IMAGE:632649 5' 5184 10276 15345 7.2 3.OOE-08 AA191195.1 EST HUMAN zq45d05xl Stratagene hNT neuron (#937233) Homo sapiens eDNA clone IMAGE:632649 5' 199 5393 5.4 2.OOE-08 AW302996.1 EST-HUMAN xr87f06.xl NCI CGAP Lu26 Homo sapiens cDNA clone IMAGE:2767139 3' 223 5416 2.7 2.OOE-08 AA425598.1 EST HUMAN zw48f07.rl Soares_totaifetus_Nb2HFQgw Homo sapiens cDNA clone IMAGE:773317 5, similar to contains AW repetitive element;contains element MER1 5 repetitive element; 488 5653 10777 0.5 2.OOE-08 AF198349.1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 644 5803 10928 3.7 2.OOE-08 AW886438.1 EST-HUMAN MRO-OT0080-240200-001- 908 OT0080 Homo sapiens cDNA 644 5803 10929 3.7 2,OOE-08 AW886438.1 EST-HUMAN MRO-OT0080-240200-001- g08 OT0080 Homo sapiens cDNA 974 6120 13.0 2.OOE-08 BE280477.1 EST-HUMAN 601155321 F1 NIH MGC 21 Homc, sapiens cDNA done IMAGE:3138893 5' 1319 6448 11614 0.7 2.OOE-08 AL163247.2 NT Homo sapiens chromosome 21 segment HS21C047 1363 6491 11656 0.9 2.OOE-08 4505538 NT Homo saplens oxytocin receptor (OXTR) mRNA 1811 6930 1.4 2.OOE-08 AW270271.1 EST-HUMAN xp43M 1.xl NCI CGAP HN1 1 Homo sapiens cIDNA done IMAGE:2743149 3' 2368 7470 12672 0.5 2.OOE-08 AW024364.1 EST-HUMAN wv03c08.xl NCI CGAF_K1d3 Homo salpiens cDNA clone IMAGE:2528462 3' similar to contains Ll.t2 Lrepetitive element; 2496 7593 0.8 2.OOE-08 K00216.1 NT Sheep His-IRNA-GLIG 3171.8320 13433 6.0 2.OOE-08 042280 SWISSPROT WNT-14 PROTEIN PRECURSOR 3171 835 13434 6.0 2.OOE-08 042280 SWISSPROT WNT-14 PROTEIN PRECURSOR 3783 8920 1.7 2.OOE-08 AW613620.1 EST HUMAN RC3-ST01 97-161099-012-b03 ST01 97 Homozapiens cIDNA 3858 8994 14093 3.7 2.OOE-08 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR.

3986 9118 14201 0.8 2.OOE-08 U82668.11 NT Homo sapiens shox gene, altematively spliced products, complete cds 4305 9429 3.4 2.OOE-08 AA459040.1 EST-HUMAN aa26c07xl NCI - CGAP_GCB1 Homo saplens cDNA done IMAGE:814380 5 similar to contains Ll.t2 L1 repetitive element., 4876 9983 2.9 2.OOE-08 AW572881.1 EST-HUMAN hel 71108.x2 NCI CGAP CM1-1 Homo sapiens cDNA done IMAGE:2919327 3' similar to contains Alu receduve e-lement;' 1493 7918 11789 1.1 1.0OE-08 P31792 SWISSPROT POL POLYPROTEIN [CONTAINS: REVERSE TRANSCRIPTASE; ENDONUCLEASE] 2005 7119 1.1 1.OOE-08 BE141959.1 EST-HUMAN PM2-HT0130-150999-001-f12 HT0130 Homo sapiens cDNA 2504 7601 12799 1.3 1.OOE-08 P33999 SWISSPROT HYPOTHETICAL 25.3 KI) PROTEIN IN RIMI-PRFC iNTERGENIC REGION 3155 8304 13413 1.2 1.0OE-08 BE246844.1 EST_HUMAN TWAP1 D5232 Pedlatric pre-B cell acute tymphoblastic leukemia Bayfor-HGSC project=TCBA Homo sapiens cDNA done TCBAP5232 3155 8304 13414 1.2 1.OOE-08 BE246844.1 EST HUMAN TCBAPID5232 Pedlatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project=TCBA Homo saplens cDNA done TCBAP5232 5191 10282 15350 1.4 1.OOE-08 AL163280.2 NT Homo sapiens chromosome 21 segment HS21 C080 4149 9275 14355 3.0 9.OOE-09 AL163279.2 NT Homo sapiens chromosome 21 segment HS21 C079 4149 9275 14356 3.0 9.OOE-09 AL163279.2 NT Homo sapiens chromosome 21 segment.HS21 C079 3444 8586 1.1 8.0OE-09. BE012076.1 EST-HUMAN RC5-BN1058-270400-031-CO6 SNI058 Homo sapiens cDNA 3555 8694 1.7 7.OOE-09 D86842.1 NT Horno saplens DNA for 3-ketoacyl-CoA thiolase beta-subunit of mitochondrial trifunctional protein, exon 2, 3 9301 14382 2.6 7.OOE-09 U89g18.1 NT Macaca mulatta homoobox protein putative pseudogene Page 69 of 196 1 Table 4

4558 9676 14768 1.0 7.OOE-09 D00649.1 NT Horno saplens gene for enteric smooth muscle gamma-achn, exon 2, 3 2107 7218 1.1 6.OOE-09 AL040439.1 EST-HUMAN DKFZp434C0514-rl 434 (synonym: htes3) Homo sapiens cDNA done DKFZp434C0514 5' 3941 9074 14157 1.1 6.OOE-09 AA557940.1 EST-HUMAN 017al 1I.sl NCI-CGAP- HSC1 Homo sapiens cDNA clone IMAGE:1040924 similar to contains Ll.t2 1.1 repetitive clement; 4906 10013 15094 4.4 6.OOE-09 BE169421.1 EST HUMAN PM1-HT0527-160200-001- h05 HT0527 Homo sapiens cDNA 1394 6521 11689 1.1 5.OOE-09 BE149264.1 EST HUMAN RC2-HT0252-120200-014h10 HT0252 Homo sapiens cDNA 510 5674 0.6 4.OOE-09 AL163282.2 NT Homo sapiens chromosome 21 segment HS21C082 949 6095 0.8 4.OOE-09 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 2379 7481 12683 2.3 4.OOE-09 AA350878.1 EST-HUMAN EST58385 Infant brain Homo saplens cDNA Vend similar to similar to heat shock protein, 90 kDa 2303 7406 12614 1.4 3.OOE-09 BE222239.1 EST HUMAN huO9eO9.xl NCI CGAF_Lu24 Homo sapiens cDNA done IMAGE:3166120 3' similar to contains MER1fi21 MER18 repetitive element; 2506 7602 12802 1.3 3.OOE-09 BE222239.1 EST-HUMAN hu09e09.xl NCI CGAP W24 Homo sapiens cDNA done IMAGE:3166120 Ysimilar to contains MER18.t3 MER18 repetitive elernent; 2614 7704 12903 1.1 3.OOE-09 P23249 SWISSPROT PROTEIN MOV-10 3287 8432 13540 1.1 3.OOE-09 BE222239.1 EST HUMAN huO9e09.xl NCLWAP-Lu24 Homo saplens cDNA done IMAGE:3166120 3'sImilar to contains MER18.1:3 MER18 repetitive element; 4329 9452 14540 3.2 3.OOE-09 AF175325.1 NT Homo sapiens eukaryofic Initiation factor 4A1 (E1F4A1) gene, partial cds 4411 9531 14620 1.5 3.OOE-09 Q9Y3R5 SWISSPROT 258.1 KDA PROTEIN C21ORF5 (K1AA0933) 795 5946 0.9 2.OOE-09 X16674.1 NT H.sapiens PADPRPM gene for NAD(+) ADP- ribosyttransferase 1237 6366 11527 1,8 2.OOE-09 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 1637 6763 3.8 2.0012-09 AL1 18573.1 EST HUMAN DKIZp761B1710-rl 761 (synonym: hamy2) Homo sapiens cDNA done DKI'Zp761131710 5' 2278 7384 12591 0.8 2.OOE-09 Q9Y3R5 SWISSPROT 258.1 KDA PROTEIN C21ORF5 (K1AA0933) 1093 6231 11382 0.9 1.OOE-09 5031624 NT ns CCAAT-box-bindis transcription factor (CBF2) mRNA 1093 6231 11383 0.9 1.0OE-09 5031624 NT Homo sapiens CCAAT-box-binding transcription factor (CF2) mRNA 1616 6742 1.0 1.OOE-09 AJ229041.1 NT Homo sapiens 959 kb config between AML1 and CBR1 on chromosome 21q22; segment 113 2453 7552 0.9 1.OOE-09 A1356086.1 EST-HUMAN qy64e11.xl NCI - WAP---Bm25 Homo sapiens cDNA done IMAGE:2016812 Ysimilar to contains MER12.1:2 MER12 repetitive element:

2860 8011 13112 1.5 1.OOE-09 U80017.1 NT Homo sapiens basic transcription factor 2 p44 (btf2p44) gene, partial cds, neuronal apoptosis Inhibitory protein (naip) and survival motor neuron protein (smn) genes, complete cds 2895 8046 13146 3.3 1.OOE-09 M28699.1 NT Homo sapiens nudeolar phosphoprotein B23 (NPM1) mRNA, complete cds 2895 8046 13147 3.3 1,OOE-09 M28699.1 NT Homo sapiens nudeolar phosphoprotein B23 (NPM1) mRNA, complete cds 2949 8100 13206 1.2 1.OOE-09 P11799 SWISSPROT MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE AND NON-MUSCLE ISOZYMES (MI.CK) [CONTAINS: TELOKIN] 4692 9804 4.3 1.OOE-09 AA719297.1 EST - HUMAN zh35b03.sl Soares_pinealgland - N31-1PG Horno sapiens cDNA done IMAGE:414029 3' similar to contains Alu repetitive elementcontains element MER22 repetitive element; __6417 11580 0.6 9.OOE-10 AW867740.1 EST HUMAN MRO-SNO040-050500-002-c07 SNO040 Homo sapiens cDNA Page 70 of 196 Table 4

2803 7955 13064 6.0 9.OOE-10 A1870071.1 we78hO3.xl Soares_Dieckgraefe colon-NHCD Homo sapiens cDNA done IMAGE:2347253 3'similar to SW:RL2Q_HUMAN P47914 60S RIBOSOMAL PROTEIN L29;contains element PTR5 repetitive element; 141 5337 10467 3,2 8.0OE-110 U63630.2 NT Homo sapiens MCM4 (MCM4) and IDNA-Pl(cs (PRKIDC) genes, partial cds 3300 8446 13554 0.9 8.0OE-110 BE080748.1 EST_HUMAN. OV1-BT0631-150200-071- f01 BT0631 Homo sapiens cDNA 4106 9232 14314 2.8 8.OOE-10 AA376832.1 EST HUMAN EST89564 Small Intestine 1 Homo sapiens ciDNA 5' end 686 5841 10976 4,2 7.OOE-10 7706225 NT Homo sapiens TPA Inducible protein (LOC515 6), mRNA 686 5841 10977 4.2 7.00EA 0 7706225 NT Horno sapiens TPA Inducible protein (LOC51586), mRNA 1605 6732 11903 0.7 7.OOE-10 Q13342 SWISSPROT LYSP100 PROTEIN (LYMPHOW- RESTRICTED HOMOLOG OF SP100) 1974 7089 1.0 7.OOE-10 P08,548 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 2512 7608 6.1 7.OOE-10 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 3049 8200 13300 2.7 7.OOE-10 X00856.1 NT H.sapien.s DHFR gene, exon 3 895 6043 11198 1.0 6.OOE-10 AJ400877.1 NT Homc, sapiens ASCL3 gene, CEGPI gene, C11orl14 gene, C111orl15 gene, C11orl16 gene and Cl 1 orfl 7 gene 2644 7733 12934 0.5 6.0OE-110 A1424405.1 EST-HUMAN tfO2d07.xl NCI-Ci3AP- Pr28 Homo saplens cDNA clone IMAGE:2095021 3' 4634 9748 2.5 6.OOE-10 AW853719.1 EST HUMAN RC3-CTO254-031099-012-912 CTO254 Horno saplens c1DNA 744 5897 1.5 5.OOE-10 AL.046804.1 EST HUMAN DKI7Zp434N219-rl 434 (synonym: htes3) Horno sapiens cDNA done DKFZp434N219 E 3430 8572 13684 1.5 5.OOE-10 Q01033 SWISSPROT HYPOTHETICAL GENE 48 PROTEIN 106 5309 3.0 4.OOE-10 A1221083.1 EST-HUMAN qg09f09.xl Soares_placenta_8togweeksL2NbHP8to9W Homo sapiens cIDNA. clone IMAGE:1759049 3' similar to contains LTRI312 LTR8 repetitive element; 1951 7068 12264 1.4 4.OOE-10 AW594709.1 EST-HUMAN hg58g03.xl NCI - CGAP - GC6 Horno sapiens cDNA done IMAGE:2949844 3'similar to contains Alu repetitive element; 2527 7623 12824 2.2 4.0OE-10 AL163303.2 NT Horno sapiens chromosome 21 segment HS21C103 3244 8391 13498 1,1 4.OOE-10 H01109.1 EST-HUMAN yj26h12.sl Scares placenta Nb2HP Homo saplens cDNA clone IMAGE:149927 Ysimilar to contains THR repetitive element; 897 6044 11200 0.6 3.OOE-10 N36113.1 EST-HUMAN yy32f06.sl Soares melanocyte 2NbHM Homo sapiens cIDNA clone IMAGE:272963 Ysimilar to contains L1A1 Ll repetitive element; 4435 9556 14649 1.1 3.0OE-1o AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 4435 9556 14650 1,1 3.OOE-10 AL-163203.2 NT Homo sapiens chromosome 21 segment HS21C003 32 5241 10346 1.4 2.00EA 0 P48988 SWISSPROT MAJOR CENTROMERE AUTOANTIGEN B (CENTROMERE PROTEIN B) (CENP-B) 32 5241 10347 1,4 2.00IE-10 P48988 SWISSPROT MAJOR CENTROMERE AUTOANTIGEN 8 (CENTROMERE PROTEIN B) (CENP-B) 1855 6973 2.1 2.OOE-10 U80017.1 NT Horno sapiens basic transcription factor 2 P44 (btf2P44) gene, partial cds, neuronal apoptosis Inhibitory protein (naip) and survival motor neuron protein (smn) genes, complete eds 1492 6619 1.3 1.OOE-10 AW867767.1 EST HUMAN MRO-SNO038-290300-001-f01 SNO038 Homo sapiens cDNA 2537 7633 0.9 1.OOE-10 AW852001.1 EST-HUMAN OVO-CTO225-191199-058-e08 CTO225 Homo sapiens cIDNA 3456 8597 13710 0.9 1.OOE-10 AW832912.1 EST HUMAN QV2-TT0003-161199-013- giO TT0003 Homo sapiens cDNA 3770 8636 0.9 1.0OE-110 AL041685.1 EST HUMAN DKFZp434N1317_rl 434 (synonym: htes3) Homo sapiens cDNA done DKFZp434N1317 5' Page 71 of 196 1 Table 4

3933 9067 5.4 1.0OE-10 AF213884.1 NT Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) gene, complete cds 4036 9164 14248 4.5 1.OOE-10 U52111.2 NT Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), dbosomal protein L18a (RPL18a), Ca2+1Calmodulin-dependent protein kinase 1 (CAMKI), creafine transporter (CRTR), WM protein (CDM), adrenoleukodystrophy protein > 4036 9164 14249 4.5 1.OOE-10 U52111.2 NT Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), ribosornal protein L1 8a (RPLI 8a), Ca2+/Calmodulin-dependent protein kinase 1 (CAMK. 1), creadne transporter (CRTR), WM protein (CDM), adrenoleukodystrophy protein > 4042 9170 14256 2.3 1.0OE-10 A13031069,1 NT Homo sapiens PC= mRNA for protein containing CXW domain 1, complete cds 4079 9206 2.1 1.OOE-10 M30629.1 NT Human pregnancy-specific glycoproteln beta-1 (SP1) mRNA, last exon 5145 10240 0.9 1.OOE-10 X87344.1 NT H.sapiens DMA, DIVIB, HLA-Zl, IPP2, LIVIP2, TAP1, LMP7, TAP2, DOB, DQB2 and RING8, 9, 13 and 14 genes 261 5448 10574 1.2 9.00EA 1 BE145600.1 EST HUMAN IL2-HT0203-291099-016- c08 HT0203 Homo sapiens cDNA 2057 7169 12375 2.0 9.00EA 1 AL134395.1 EST-HUMAN DKFZp547D225-rl 547 (synonym: hfbrl) Horno sapiens cDNA done DMp5471)225 5' 2057 7169 12376 2,0 9.00EA 1 AL134395.1 EST-HUMAN WZp547D225-rl 547 (synonym: hfbrl) Homo sapiens cDNA done DKFZp547D225 5' 3337 8481 13596 2.3 9.00EA 1 AL1 34395.1 EST HUMAN DI(Rp5471)225-rl 547 (synonym: hfbrl) Homo sapiens cDNA clone WZp5471)225 5' 3337 8481 13597 2.3 9.00EA 1 AL134395.1 EST HUMAN DKFZp5471)225-rl 547 (synonym: hfbrl) Homo sapiens cDNA clone DKRp547D225 5' 4400 9F21 14611 1.1 9.00EA 1 AA775985.1 EST HUMAN ae78f0I.sl Stratagene schizo brain S1 1 Horno, sapiens cDNA done IMAGE:970297 X 3078 8229 10.0 8.00EA 1 H19971.1 EST HUMAN yn5W 1.sl Scares adult brain N2b5HB5SY Homo sapiens cDNA done IMAGE:172173 X similar to contains L1 repetitive element:

3881 9017 14112 0.8 8.00EA 1 A1478617.1 EST-HUMAN tm54c09.xl NCI_PGAP Kidl 1 Homo sapiens cDNA done IMAGE:2161936 3' 3957 9090 14173 4.0 8.00EA 1 N23712.1 EST HUMAN yw46e06.sl Weizmann Olfactory Epithelium Homo sapiens cDNA clone IMAGE:255298 3, 1431 6558 11734 0.8 7.00EA 1 AA330642.1 EST HUMAN EST34392 Embryo, 6 week 1 Homo saplens cDNA Vend 406 5574 10712 1.8 6.00EA 1 M55270.1 NT Human matrix Gla protein (MGP) gene, complete cds 406 5574 10713 1.8 6.00EA 1 M55270.1 NT Human matrix Gla protein (MGP) gene, complete cds 11 5219 10320 0.9 5.00EA 1 AL163283.2 NT Homo sapiens chromosome 21 segment HS21 C083 3320 5219 10320 1.5 5.00EA 1 AL163283.2 NT Homo saplens chromosome 21 segment HS21C083 4135 9261 14343 1.5 5.00EA 1 P48034 SWISSPROT ALDEHYDE OXIDASE 1380 6507 1,4 4.00EA 1 AA436042.1 EST-HUMAN zu01b12xl Soares tesfis_NHT Homo sapiens cIDNA done IMAGE:730559 5' 4518 9637 14728 1.4 4.00EA 1 D44666.1 EST HUMAN HUMSUPY069 Human brain cIDNA Homo sapiens cDNA done 069 1476 11777 08 3.00EA 1 6679077 NT Mus musculus expressed In non- metastafic cells 2, protein (NIVI23B) (Nme2), mRNA 1 9 9305 1.5 3.00EA 1 AA309248.1 EST-HUMAN EST180120 Liver, hepatocellular carcinoma Homo saplens cDNA S' end 4 79 61603 11248 0.5 2.0012-11 AI 150502.1 EST-HUMAN qf36d04.xl Scares - testis - NHT Homo saplens cDNA done IMAGE:1 752102 Ysimilar to 6091 contains MER1O.t3 MER10 repetitive element, 091 Page 72 of 196 Table 4

1168 6302 11458 1.2 2.00EA 1 R24807.1 EST HUMAN yg43e12xl Soares Infant brain 1 NIB Homo sapiens cDNA done IMAGE:35144 5' 1168 6302 11459 1.2 2.00EA 1 R24807.1 EST HUMAN yg43e12xl Soares Infant brain 1 NIB Horno sapiens cDNA done IMAGE:35144 S' 1596 6722 11891 1.4 2.OOE-11 L17432.1 NT Gallus ggilus rho-globin, beta-H globin, beta-A globin, epsilon-globin, and olfactory receptor like protein CORYbeta (CORYbeta) genes, complete ods 1596 6722 11892 1.4 2.OOE-11 L17432.1 NT Gallus gallus rho-globin, beta-H globin, beta-A globin, epsilon-globin, and olfactory receptor like protein CORYbeta (COR3'beta) genes, complete cds 1600 6727 11897 1.0 2.00EA 1 A1126371.1 EST HUMAN qc51c10.xl Soares_pregnant - uterus - NbHPU Homo sapiens cDNA done IMAGE:1713138 3' similar to gb102932 PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA (HUMAN);contains 1.111 L1 repetitive element; 2737 7821 13025 1.1 2.00EA 1 AF087913.1 NT Human endogenous retrovirus HEW-P-T471) 3160 8309 13421 4,2 2.00EA 1 P10263 SWISSPROT RETROVIRUS-RELATED GAG POLYPROTEIN (VERSION 1) 3284 8429 13537 0.9 2.00EA 1 A1478617.1 EST HUMAN tm54c09.xl NCI CGAP Kidl 1 Homo sapiens cDNA clone IMAGE:2161936 3' 3452 8593 1.0 2.00EA 1 AF020503.1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 4501 9621 1.0 2.OOE-11 AL163227.2 NT Homo sapiens chromosome 21 segment HS21 C027 4851 9958 5.5 2.00EA 1 BE062558.1 EST HUMAN QV2-ST0258-26109M14-a01 BT0258 Homo sapiens cDNA 4961 10064 15136 1.3 2.00EA 1 AA307331.1 EST HUMAN EST178226 Colon carcinoma (HCC) cell line Homo saplens cDNA 5' end similar to similar to alpha-2-ma=globulin 660 5816 10944 1.2 1.00EA 1 AJ131016.1 NT Horno sapiens SCL gene focus 1199 6331 11486 0.9 1.0012-11 AL163279.2 NT Homo sapiens chromosome 21 segment HS21C079 1249 6378 11537 1.6 1.00EA 1 AA489871.1 EST-HUMAN ab01b12.rl Stratagene fetal retina 937202 Homo sapiens cDNA done IMAGE:839519 5' similar to contains OFRA2 MER1 0 repetitive element; 1483 6610 0.7 1.00IE-11 1 AF1 19914.1 NT Horno sapiens PR03078 mRNA, complete cds 1838 6957 1,2 1.0OE-1 1 A13030947.1 NT Rattus norvegicus mRNA for highaffinity choline transporter CHT1, complete cds 2079 7191 12394 1.1 1.0012-11 AF000573.1 NT Homo sapiens homogendsate 1,2- dioxygenase gene, complete cds 3454 8595 13706 1.3 1.00EA 1 BE004315.1 EST - HUMAN CMO-BN0105-170300-292- d12 BN0105 Homo saplens cDNA 3798 8935 14028 27.7 1.0012-11 A13001523.1 NT Homo sapiens gene for TMEM1 and PWP2,complete and partial cds 3798 8935 14029 27.7 1.00EA 1 ABOO1523.1 NT Homo sapiens gene for TMEM1 and PWP2,complete and partial cds 2922 8073 13181 0.8 9.OOE-12 P20742 SWISSPROT PREGNANCY ZONE PROTEIN PRECURSOR 4562 9680 14773 2.8 7.OOE-12 Q05904 SWISSPROT 34 KI) SPICULE MATRIX PROTEIN PRECURSOR (LSM34) 4252 9377 14457 7.9 6.OOE-12 AA732516.1 EST_HUMAN nz88fl 1M NCLWAP-GC131 Horno sapiens oDNA done IMAGE:1302573 3'sImilar to contains Alu repetitive element; 1027 6167 11322 1 0 5.OOE-12 TO6573.1 EST-HUMAN EST04462 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA done HFBDV33 3344 8488 13602 1.2 5.OOE-12 BE047779.1 EST - HUMAN tz42b05.yl NCI_WAP 8m52 Homo sapiens cDNA done IMAGE:2291217 5' 3666 8805 13899 5,9 5.OOE-12 AJ271736.1 NT Homo sapiens Xq pseudoautosomal region; segment 212 5171 10265 0.8 5.OOE-12 AA720661.1 EST-HUMAN nw24bl 1.si NCI CGAP GWO Horno saplens cDNA done IMAGE:1241373 3' 244 5433 10559 1.2 4.OOE-12 AA700326.1 EST-HUMAN zj74g11 1M Soares- fetaLliveLspleerLINFLS-S1 Horno sapiens cDNA clone IMAGE:460676 3' 245 5433 10559 1.2 4.OOE-12 AA700326.1 EST-HUMAN zj74g11 I.sl Soares_fetaUiver-sploon-1 NFI-S-SI Homo saplens cDNA done IMAGE:460676 3' 13380 2.2 4.OOE-12 004791 SWISSPROT _ FATTY ACYL-COA HYDROLASE PRECURSOR, MEDIUM CHAIN (THIOE E Page 73 of 196 1 Table 4

3126 8275 13381 2.2 4.OOE-12 Q04791 SWISSPROT FATTY ACYL-COA HYDROLASE PRECURSOR, MEDIUM CHAIN (THICESTERASE 8) 4520 9639 14730 0,9 4.OOE-12 A1689984.1 EST - HUMAN bc26h05.xl NCLWAP- LU24 Homo sapiens cIDNA done IMAGE:2270745 Ysimilar to TR:013539 013539 MARINER TRANSPOSASE.; 420 5209 10310 1,3 3.OOE-12 042163 SWISSPROT COCHLIN PRECURSOR (COCH-5B2) 600 5758 10877 1.3 3.0OE-12 AW341683.1 EST HUMAN hdl 3d01.xl Soares-NFL.. GBC-Sl Homo sapiens cDNA clone IMAGE:2909377 3'slmilar to TR:014517 014517 SIVIRP.; 600 5758 10878 1.3 3.OOE-12 AW341683.1 EST-HUMAN hd13d017Soares_NFL - T - 13121Q_S1 Horno sapiens cDNA clone IMAGE:2909377 3'slmilar to TR:01 4517 014517 SIVIRP.; 2864 8015 13117 1.4 2.OOE-12 AW341809,1 EST HUMAN ha02d05M NCLWAP W24 Homo sapiens cDNA done IMAGE:2872521 3' 2864.8015 13118 1,4 2.OOE-12 AW341809.1 EST HUMAN haO2d05.xl NCI C2AP Lu24 Horno sapiens cDNA done IMAGE:2872521 3' 3421 8563 13675 1.0 2.OOE-12 6754495 NT Mus musculus keratin-assoclated protein 6.2 (Kriap6-2), mRNA 4025 9153 14236 1.0 2.OOE-12 J01884.1 NT Rat U3A small nuclear RNA 4025 F-53 14237 1.0 2.OOE-12 J01884.1 NT Rat U3A small nuclear RNA 4288 8015 13117 3.3 2.OOE-12 AW341809.1 EST HUMAN ha02d05.xl NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE:2872521 3 4288 6015 13118 3.3 2.OOE-12 AW341809.1 EST HUMAN haO2d05.xl NCI CGAP Lu24 Homo saplens cDNA done IMAGE:2872521 3' 4324 9"7 1.8 2.OOE-12 BE063509.1 EST-HUMAN CMO-BT0281-031199-087-a03 BT0281 Homo sapiens cDNA 4804 9914 15005 0.8 2.OOE-12 070306 SWISSPROT TBX15 PROTEIN (T-BOX PROTEIN 15) 4804 9914 15006 0.8 2.0OE-112 070306 SWiSSPROT T131X15 PROTEIN (T-BOX PROTEIN 15) 117 5317 10446 0.6 1.OOE-12 AW627674.1 EST HUMAN hh90a09.xl NCLWAP_GU1 Horno sapiens cDNA done IMAGE:2970040 3'sImilar to contains MER1 8.tl MER1 8 repetitive element; 1944 7061 0,7 1.OOE-12 A1871726.1 EST HUMAN wm51f07.xl NCI-CGAP_Ut2 Homo saplens cDNA done IMAGE:2439493 Ysimilar to contains Ll.b3 L1 repetitive element; 3032 8184 13284 1.2 1.OOE-12 AF000991.1 NT Homo sapiens tests-specific Testis Transcript Y 2 C) mRNA, partial cds 3032 8184 13285 1.2 1.0OE-12 AF000991.1 NT Homo saplens tesfis-spedfic Testis Transcript Y 2 (TTY2) mRNA, partial cds 3575 8714 1.1 9.OOE-13 AJ271735.1 NT Homo sapiens Xq.pseudoautosomal region, segment 112 3865 9001 14102 1.2 9.OOE-13 AB029900.1 NT Homo sapiens CST gene for cerebroside sulfotransferase, exon 1, 2. 3, 4, 5 700 5855 10990 1.7 8.OOE-13 U29185.1 NT Homo saplens prion protein (PrP) gene. complete cds 700 5855 10991 1.7 8.00IE-13 U29185.1 NT- Homo saplens prion protein (PrP) gene, complete cds 1796 6916 12102 1.4 8.OOE-13 U80017.1 NT Homo sapiens basic transcription factor 2 p44 (btf2p44) gene, partial cds, neuronal apoptosis Inhibitory protein (naip) and survival motor neuron protein (smn) genes, complete cds 2054 7166 12372 6.7 6.OOE-13 AL163207.2 NT Homc sapiens chromosome 21 segment HS21 C007 1815 W-4 12123 1.8 5.OOE-13 Q04899 SWISSPROT SERINE/THREONINE-PROTEIN KINASE PCTAIRE-3 1815 6934 12124 1.8 5.OOE-13 Q04899 SWISSPROT SERINE/THREONINE-PROTEIN KINASE PCTAIRE-3 3280 8425 1.1 5.OOE-13 R78338.1 EST HUMAN yi82f04.rl Soares placenta Nb21- 1P Homo sapiens cDNA done IMAGE:145759 5' 3351 8495 1.5 5.OOE-13 AA435773.1 EST-HUMAN zt77a12.sl Soares_tesds_NHT Homo sapiens cDNA done IMAGE:728350 3' similar to contains Alu repetitive element;contalns element MER22 repetitive element; 1824 6943 1,1 4.OOE-13 AW378614.1 EST HUMAN PM2-HT0224-221 099-001 -ell HT0224 Horno sapiens cDNA 2411 7510 0.6 4.OOE-13 AF003529.1 NT Homo saffiens glypican 3 (GPC3) gene, pardal cds and flanking repeat regions 173 5366 1.3 3.OOE-13 AF003528.1 NT Homo sapiens X-linked anhidroific ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions Page 74 of 196 Table 4

847 5996 1 3.OOE-13 AA430310.1 EST HUMAN zw68908.rl Soares tesfls_NHT Horno sapiens eDNA done IMAGE:781406 5' 2319 7422 12631 1.3 3.OOE-13 AJ271736.1 NT Homo sapiens Xq pseudoautosomal regiow, segment 212 2429 7528 1.0 3.OOE-13 AL163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 3150 8299 2.9 3.OOE-13 AA745844.1 ESTHUMAN obl8d02.sl NCIúGAP K1d5 Homo sapiens DNA done IMAGE:1324035 3' 144 5340 10470 1,0 2.OOE-13 U52111.2 NT Horno sapiens X28 region near ALD lows containing dual specificity phosphatase 9 (DUSP9), dbosomal protein Ll 8a (RPL1 8a), Ca2+1Calmodulin-dependent protein kinase 1 (CAMKI), creatine transporter (CRTR), WM protein (CDM), adrenoleukodystrophy protein > 239 54F9 10556 1.3 2.0012-13 U23839.1 NT Danio rerio fibroblast growth factor receptor 4 mRNA, complete cds 4019 9148 1.7 2.OOE-13 A1-163278.2 NT Horno sapiens chromosome 21 segment HS21 C078 4488 9608 14701 1.5 2.OOE-13 AF135116.1 NT Homo sapiens NF-IE2-related factor 3 ge, complete cds 288 5474 10604 1.4 1.OOE-13 S74129.1 NT FGF-l=fibroblast growth factor 1 [human, kidney, Genom, 342 nt, segment 2 1 of 21 870 6018 11178 1.5 1.OOE-13 AJ007973.1 NT Homo saplens LGIVID213 gene 1228 6358 1.2 1.OOE-13 AL163213.2 NT Homo sapiens chromosome 21 segment HS21 C01 3 1314 6442 11609 1,3 1.OOE-13 X87344.1 NT H.sapiens DMA, DMB, HLA21, W122, LIVIP2, TAP1, LIVIP7, TA122, DOB, DQB2 and RING8, 9. 13 and 14 genes 1976 7091.12293 0.8 11.0OE-13 AA720574.1 ESI-HUMAN nw21902.sl NCI- CGAP_GCBO Homo saplens cDNA clone IMAGE:1241138 3'similar to contains THR.t3 THR repetitive element; 330 5511 10637 1.0 9.OOE-14 AA781159.1 EST HUMAN aj24c01.sl Soares tests NHT Homo sapiens cDNA clone 1391232 Ysimilar to contains MER19.M MER119TepefiCve element; 331 5512 10638 1.0 9.0OE-14 AA781159.1 EST-HUMAN aj24c01.sl Soares_testis- NHT Homo saplens cDNA clone 1391232 3'sImilar to contains MER1 9.M MER1 9 repetitive element; 2451 7550 13014 1.5 9.0012-14 AW861577.1 EST HUMAN RC4-CT0322-080100-013- d09 CT0322 Homo sapiens cIDNA 2725 0 1.7 9.0012-14 AW513296.1 EST HUMAN xo54h05.xl NCI CGAP Utl Homo saplens cDNA clone IMAGE:2707833 3' 7809 8 3073 2 13322 3.7 9.OOE-14 AW513296.1 EST-HUMAN xo54h05.xl NCI CGAP Utl Homo sapiens cIDNA clone IMAGE:2707833 3 8224 4 2 3200 51 1 10637 1.0 9.OOE-14 AA781159.1 EST-HUMAN aj24c01.sl Soares_testis NHT Homo sapiens eDNA clone 1391232 Ysimilar to contains 5511 MER1 9M MER1 9 repetICve element; 81865 3728 13958 5.2 9.OOE-14 D14547.1 NT Human DNA, SINE repetitive element 4650 9762 14857 1.9 9.OOE-14 AJ002153.1 NT Saguinus oedipus gene for seminal veside secreted protein semenogelin 1 3453 8594 1.6 8.OOE-14 BE468263.1 EST HUMAN hz71c09.xl NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE:3213424 3' 3872 9008 2.8 8.OOE-14 R76269.1 EST HUMAN y172c03.rl Soares placenta NWHP Homo sapiens cIDNA clone IMAGE:144796 3' 1611 7921 1.1 7.OOE-14 AW151673.1 EST-HUMAN 067e10.xl NCI - CGAP_Gas4 Homo sapiens cIDNA done IMAGE:2623146 Ysimilar to contains MER10.Q MERIO repetitive element; 363 5541 10672 3,5 6.OOE-14 AF020503.1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 4861 9968 15053 1.0 6.OOE-14 T90961.1 EST HUMAN yd58a12.si Soaresfetal liverspleen INFLS Homo sapiens cDNA done IMAGE:112414 3, 5114 10210 15288 1.0 6.OOE-14 8923548 NT Homo sapiens hypothetical protein FLJ20585 (FU20585), mRNA 5114 10-210 15289 1.0 6.OOE-14 8923548 NT Homo saplens hypothetical protein FU20585 (FLJ20585), mRNA C Page 75 of 196 Table 4

57601 CANALICULAR MULTISPECIFIC ORGANIC ANION TRANSPORTER 1 (MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 2) (CANALICULAR MULTIDRUG RESISTANCE 602 Q63120 SWISSPROT PROTEIN) 10880 1.3 5.OOE-14 xb03b05.wl- NCI CGAP_GU1 Horno saplens cDNA done IMAGE:2575185 3'similar to 4985 10088 15160, 1,1 5.OOE-14 AW073791.1 EST HUMAN contains L1A2 Ll repetitive element; 1107 7907 0.6 4.OOE-14 P04928 SWISSPROT S-ANTIGEN PROTEIN PRECURSOR 1835 6954 12140 2.4 4.OOE-14 AJ007973.1 NT Horno, sapiens LGIVID213 gene 3693 8832 14405 0.9 4.OOE-14 AA046502.1 EST HUMAN zk67a06xl Soares_pregnant uterus_NbHPU Homo sapiens cDNA clone IMAGE:487858 5' 4194 9320 1.0 4.OOE-14 N46328.1 EST HUMAN yy73c12.sl Soares-muldpie- sderosis-2NbHMSP Homo sapiens cDNA done IMAGE:279190 Ysimilar to contains L1A3 L1 repetitive element; 934 6081 11237 0.7 3.OOE-14 X95466.1 NT R.norvegicus mRNA for CPG2 protein cDNA done IMAGE:2743343 Ysimilar to 4841 9948 15037 0.8 3.OOE-14 AW265354.1 EST-HUMAN xp45f112.xl NCI_PGAP HN1 1 Horno saplens 4844 9951 15039 1.1 3.OOE-14 7656864 NT contains Alu repetitive jiement:contains element MER9 repetitive element; Homo sapiens a disintogfin and metalloproteinase domain 29 (ADAM29), mRNA hx901 1.xl NCI-CGAP-Kidl 1 Homo saplens cDNA clone IMAGE:3195501 Yslmilar to 5104 10200 1.2 3.OOE-14 BE4663711 EST-HUMAN contains MER412 MER4 re2efifive element; PROTEIN PRECURSOR (CS) 5157 10252 15323 1.5 3.OOE-14 P02894 SWISSPROT CIRCUMSPOROZOITE region; segment 212 384 5553 10686 1.4 2.OOE-14 AJ271736.1 NT Homo sapiens Xq pseudoautosomal 384 5553 10687 1.4 2.OOE-14 AJ271736.1 NT Homo, sapiens Xq pseudoautosomal region; segment 212 675 795 960 2.2 2.OOE-14 AL163303.2 NT Horno sapiens, chromosome 21 segment HS21 Cl 03 BT0377 Homo saplens cDNA 2339 74-4-1 1.4 2.OOE-14 AW372868.1 EST_HUMAN RC5-BT0377-091299-031-DI2 tumor deletion region protein 1 (RTDR1), mRNA 2416 7515 12778 1.1 2.OOE-14 7657529 NT Homo sapiens rhabdoid 2479 7577 1.2 2.OOE-14 AL163209.2 NT Homo sapiens chromosome 21 segment HS21C009 TRANSCRIPTASE HOMOLOG 2640 7729 11342 1.0 2.OOE-14 P08548 SWISSPROT LINE-1 REVERSE 1051 6190 1.3 11.0OE-14 AL1 63246.2 NT Homo, sapiens chromosome 21 segment HS21C046 HS21C068 21 1384 6511 11675 2.8 1.OOE-14 AL163268.2 NT Homo sapiens chromosome segment HS21 C068 21 1384 6511 11676 2.8 11.0OE-14 AL1 63268.2 NT Homo, sapiens chromosome segment X from filamin (FLN) gene to glucose-6-phosphate Homo sapiens chromosomeregion 1956 7073 12270 7.8 1.OOE-14 L44140,1 NT dehydrogenase (G6PD) gene, complete ods's 2137 7247 12453 2.2 1.0011-14 AL1 63303.2 NT Horno, sapiens chromosome 21 segment HS21 Cl 03 2358 T460 12666 2.3 1.0OF-14 AF001689.1 NT Homo sapiens ribosomal protein L23A (RPL23A) gene, complete cds PROTEIN PRECURSOILCLONE PFHRP-11) 2914 806 13173 1,4 1.OOE-14 P05227 SWISSPROT HISTIDINE-RICH schizo brain S11 Homo sapiens cDNA clone IMAGE:9713503' 3811 8948 14044 2.0 1.0OE-14 AA682994.1 EST HUMAN ae89c12.sl Stratagene NCI CGAP Lu28 Homo, sapiens cDNA clone IMAGE:2753059 3' 4374 9496 14583 1.7 1.OOE-14 AW27585i.A EST HUMAN xq39h10.xl phosphatase, receptor type, T (PTIPRT), mRNA 1558 6685 118521-. 9.0ut-15 7427522 NT Homo sapiens protein tyrosine 1703 6826 12007 1.2 9.OOE-15 AL163283.2 NT Homo sapiens chromosome 21 segment HS21C083 1703 6826 12008 1.2 9.OOE-15 AL1 63283.2 NT Homo saplens chromosome 21 segment HS21C083 \_J Page 76 of 196 Table 4

F -1 - -1 2126 7236 0.6 9.OOE-15 AF196779.1 NT Homo sapJens transcription factor IGHM enhancer 3, JMI 1 protein, JM4 protein, JM5 Protein, T54 protein, JMI10 protein, A4 differenflationTdependent protein, triple LIM domain protein 6, and synaptophysin genes, complete cds; and L-typ6 calcium channel a> 2780 5637 1,0 8.0OE-115 BE261482.1 EST HUMAN 601148632F1 NIH MGC 19 Homo sapiens cDNA done IMAGE:3164023 5' 978 6123 11280 2.1 6.OOE-15 AJ271736.1 NT Homo sapiens Xq pseudoautosomal region; segment 212 404 5572 10709 2,0 5.OOE-15 AL163208.2 NT Homo sapiens chromosome 21 segment HS21 C008 2733 7817 13022 1.4 5.0012-15 U91328.11 NT Human hereditary haemochromatosis region, histone 2A-like protein gene, hereditary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete ods 3423 8565 1,0 5.0OE-115 AW296817.1 EST HUMAN U1-1-1-13WO-ajb-g-1 0-0-Ul. sl NCI-CGAP_Sub6 Homo sapiens cDNA done IMAGE:2731219 3' 421 5210 10311 0,9 4.OOE-15 A1-163303.2 NT Homo sapiens chromosome 21 segment HS21 Cl 03 2612 7702 3.0 4.0OE-15 H5561 1.1 EST HUMAN CHR220550 Chromosome 22 exon Homo sapiens cDNA done C22 749 V 4039 9167 14252 0.8 4.OOE-15 AL1 18596.1 EST HUMAN DKFZp761C0810-rl 761 (synonym: hamy2) Homo sapiens cbNA clone DKFZp761C0810 5, 4123 9249 5.9 3.OOE-15 N89452.1 EST HUMAN LY1 142F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA done LY1 142 6 similar to ANF(CARDIODILATIN) 4843 9950 1.4 3.OOE-15 P92485 SWISSPROT NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5 4955 10060 15131 0.9 3.OOE-15 AA078097.1 EST-HUMAN 7P01F03 Chromosome 7 Placental cDNA Library Homo sapiens cDNA done 7P01F03 4955 10060 15132 0.9 3.OOE-15 AA078097.1 EST-HUMAN 7P01 F03 Chromosome 7 Placental cDNA Library Homo sapiens cDNA done 7P01 F03 250 5438 10565 1.1 2.OOE-15 AF223391,1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 364 5542 10673 1.1 2.OOE-15 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 364 5542 10674 1.1 2.0012-15 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49. and partial cds, alternatively spliced 1512 6639 1.1 2.0OE-115 8923201 NT Homo sapiens hypothetical protein FLJ20212 (FLJ20212), mRNA 3465 8606 13718;1 1.0 2.OOE-15 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cels, alternatively spliced 3465 8606 13719 1.0 2.OOE-15 AF223391.1 NT Horno sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 4522 9641 2.1 2.OOE-15 A1806335.1 EST HUMAN wf07f06.xl Soares-NFL,GBC-SI Homo saplens cDNA clone IMAGE,2349923 Ysimilar to TR:Q61043 061043 NINEIN---.

5097 10194 15273 1.3 2.OOE-15 P13993 SWISSPROT REPETITIVE PROLINE-RICH CELL WALL PROTEIN 2 PRECURSOR 5097 10194 15274 1.3 2.OOE-15 P13993 SWISSPROT REPETITIVE PROLINE-RICH CELL WALL PROTEIN 2 PRECURSOR 2747 7831 0.7 1.0OE-15 A1689984.1 EST-HUMAN tx26h05.xl NCl-WAP_Lu24 Homo sapiens eDNA done IMAGE:2270745 3' similar to TR:Q13539 Q13539 MARINER TRANSPOSASE, 2979 8130 13238 0.8 1.OOE-15 BE043584.1 EST-HUMAN hk40e02y11 NCI_PGAP 0v34 Homo sapiens cDNA clone IMAGE:2999162 5, 3103 8253 13357 1.0 1.OOE-15 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 42^ 9387 14471 0.8 1.OOE-15 SE182696.1 EST HUMAN RC3-HT0649-100500-022- b05 HT0649 Homo sapiens cDNA Page 77 of 196 1 Table 4

5086 10184 15263 1.2 1.00EA 5 A1984928.1 EST HUMAN wr86e04.xl NCI CGAP Kidl 1 Homo sapiens ciDNA done IMAGE:2494590 3' 4404 9524 14614 1.0 9.OOE-16 4503168 NT Homo sapiens cut (Drosophilalike 1 (CCAAT displacement protein) (CUTL1) mRNA 2094 7205 3,0 6.OOE-16 AW97261 1.1 EST-HUMAN EST384702 MAGE resequences, MAGI- Homo, sapiens cIDNA 1477 6604 11778 1.1 5.00EA 6 AJ251154.1 NT Mus musculus olfactory receptor duster, OR37A, OR37B, OR37C, OR37E genes and OR37D pseudogene 2647 7736 12936 0.7 5.OOE-16 AA992176.1 EST-HUMAN ot80c04.sli Soares- totai-fetus_Nb2HFQ_gw Homo sapiens ciDNA done IMAGE:1623078 3' similar to contains element 1.1 repetitive element:

2192 7301 1.3 4.OOE-16 A13001523.1 NT Homo sapiens gene for TMEM1 and PWP2,complete and partial ods 2328 7431 12639 1,3 4.OOE-16 AW797168.1 EST-HUMAN OV1-UM0036-200300-115- g02 UM0036 Homo sapiens cIDNA 2328 7431 12640 1.3 4.OOE-16 AW797168.1 EST HUMAN QV1-UM0036-200300-115- 902 UM0036 Homo sapiens cIDNA 3411 8553 13661 3.9 4.OOE-16 Q16653 SWISSPROT MYELIN-OLIGODENDROCYTE GLYCOPROTEIN PRECURSOR 4050 9177 14260 3.6 4.OOE-16 BE083875.1 EST HUMAN PM4-BT0650-010400-002- 909 BT0650 Homo sapiens cIDNA 4050 9177 14261 3.6 4.OOE-16 BE083875.1 EST HUMAN PM4-13T0650-010400-002g09 BT0650 Homo sapiens cIDNA 5092 10190 15269 1.0 4.0OE-116 P08548 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 128 5324 10456 0.5 3.OOE-16 AW022862.1 EST-HUMAN df45c011.yl Morton Fetal Cochlea Homo sapiens cIDNA clone IMAGE:2486376 5, 128 5324 10457 0.5 3.OOE-16 AW022862.1 EST-HUMAN df45c01.yl Morton Fetal Cochlea Homo sapiens cIDNA done IMAGE:2486376 5, 458 5624 1.5 3.OOE-16 AL046445.1 EST HUMAN DKFZp434P037-rl 434 (synonym: hie s3) Homo sapiens cIDNA clone DIKFZp434P037 5' 467 5632 0.6 3.0OE-116 AF135446.1 NT Homo sapiens TSX (TSX) pseudogene, exon 5 1436 6563 11739 0.7 3.0OE-116 028983 SWISSPROT ZONADHESIN PRECURSOR 2946 8097 13202 4.1 3.OOE-16 P03200 SWISSPROT ENVELOPE GLYCOPROTEIN GP340 (MEMBRANE ANTIGEN) (MA) [CONTAINS:

GLYCOPROTEIN GP220] 3756 8894 13985 1.1 3.00EA 6 BE252133.1 EST HUMAN 601113712F1 NIH MGC 16 Homo sapiens ciDNA done IMAGE:3354291 5' 3874 9010 8.2 3.0012-16 AF020503.1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 3875 9011 1.0 3.0OE-116 U03887.1 NT Human BXP20 gene 956 6102 1.2 2.0OE-11 6 AL1 63279,2 NT Homo sapiens chromosome 21 segment HS21 C079 2337 7439 1.0 2.0OE-116 AA621761.1 EST-HUMAN af06d04.sl Soares testis NHT Homo sapiens cDNA done IMAGE:1030855 3' 2657 7746 0.6 2.0OE-116 J03061.1 NT Human SSAV-related endogenous retroviral LTR-like eleTent 4087 9213 14296 1.3 2.OOE-16 X8921 1.1 NT H.sapiens DNA for endogenous retroviral like element 1 178 5371 10495 0.9 11.0OE-16 AF2007119.1 NT Homo sapiens pituitary tumor transforming gene protein (PTTG) gene, complete ods 377 5583 7.6 1.0OE-116 AA628592.1 EST-HUMAN af39g11.sl Soares - total - fetus - Nb21-11F8 - 9w Homo sapiens cIDNA done IMAGE:1034084 3' similar to contains OFR.t2 OFR repetitive element; 4345 9468 14556 1.2 1.0OE-16 P10272 p POL POLYPROTEIN (CONTAINS: PROTEASE; REVERSE TRANSCRIPTASE; SWISSPROT ENDONUCLEASE] 3679 8818 13911 2.5 9.OOE-17 AW900048.1 EST HUMAN CM1-NN1003-200300-153- e01 NN1003 Homo sapiens cIDNA 1001 6141 0.6 8.OOE-1 7 AW880701.1 EST-HUMAN OVO-OT0032-080300-155-d01 OT0032 Homo sapiens cIDNA 3817 8954 0.9 8.OOE-17 AL163280.2 NT Homo sapiens chromosome 21 segment HS21 C080 959 6104 11262 0.9 7.OOE-17 Q91081 SWISSPROT ALPHA-2 ADRENERGIC RECEPTOR (ALPHA-2 ADRENOCEPTOR) 1442 6569 1.2 7.0012-17 6753097 NT Mus musculus apollpoproteln B editing complex 2 (Apobec2), mRNA 10518 1.6 6.OOE-17 AW983880.1 1ESTHUMAN RC1-HNO003-220300-021-b04 HNO003 Homo sapiens cIDNA Page 78 of 196 415 5204 10305 0.8 5.OOE-17 T641 10.1 EST HUMAN yc05h08xl Stratagenelung (#937210) Homosapien. CIMAclone irvi^tzr-.[tvo.2uw Homo sapiens cDNA done IMAGE:3141678 S' 2413 7512 12719 0.9 4.OOE-17 BE315238.1 EST HUMAN 601141631 F1 NIH-MGC-9 - -fl-6-3 12368 1.4 3.OOE-17 AW119123.1 EST HUMAN W89c09.xl Soares NFL T G13C_SI Homo sapiens cDNA clone IMAGE:2604784 3' 2051 PROTEIN-COUPLED RECEPTOR MRG 3157 8306 1.3 3.OOE-17 P35410 SWISSPROT MAS-RELATED G cDNA done IMAGE:3181999 3' Homo 3590 8729; 13825: 1.1 3.OOE-17 BE326522.1 EST HUMAN hw05b04.xl NCI WAR- Lu24 saplens Homo sapiens cDNA done IMAGE:3181999 3' 3590 8729 13 26 1.1 3.OOE-17 BE326522.1 EST HUMAN hwO5b04.xl NCI CGAP_LUZ4 Horno sapiens cDNA done IMAGE:1959922 Ysimilar to qt63a06,xl NCI-CGAP_Eso2 350 5531 10659 1.0 2.OOE-17 A1270080.1 EST HUMAN contains Alu repetitive element; Homo sapiens cDNA done IMAGE:1959922 Ysimilar to qt63a06.xl NCI CGAP_Eso2 351 5531 10659 0.7 2.OOE-17 A1270080.1 EST HUMAN contains Alu reeuuve element; 972 7497 12702 1.3 2.OOE-17 AA722932.1 EST HUMAN zg81d04.sli Soaresfetal_heart-NbH1-119W Horno sapiens cDNA clone IMAGE:399751 3' 2397 7497 12703 u -2.OOE-17 Q28983 SWISSPROT ZONADHESIN PRECURSOR 2397 0.9 2.OOE-17 028983 SWiSSPROT ZONADHESIN PRECURSOR TRIPLET H PROTEIN (200 KDA NEUROFILAMENT PROTEIN) NEUROFILAMENT 2899 8050 13153 5.6 2.OOE-17 P12036 SWISSPROT (NEUROFILAMENT HEAVY POLYPEPTIDE) (NF-H) 4681 9793 -14895 1.1 2.OOE-17 P80668 SWISSPROT PHENYLACETALDEHYDE DEHYDROGENASE (PAD) RESISTANCE PROTEIN 1 ( LYCOPROTEIN 733 5887 11031 -1.2 1.OOE-17 P08183 SWISSPROT MULTIDRUG 1729 6852 12031 0.9 1.0OE-17 AL163207.2 NT Homo sapiens chromosome 21 segment HS21 C007 CHAIN PRECURSOR 2069 7181 12385 0.6 1.OOE-17 P02461 SWISSPROT COLLAGEN ALPHA 1(111) promoter reRI2d exons 1 A and 1 B 2 (THI3S2) 2287 7392 12599 0,5 1.OOE-17 U79410.1 NT Homo sapiens thrombospondin gene, mannosidase, beta A, lysosomal (MANBA) gene, and ubiquifin-conjugafing Horno sapiens 3519 8660 12728 1.0 1.OOE-17 AF224669.1 NT enzyme E2D 3 (U13E2D3) genes, complete ods 4045 9172 13947 7.2 1.OOE-17 R09942.1 EST HUMAN yf30e07xl Scares fetal liver spleen I NFLS Horno sapiens cDNA done IMAGE: 128388 5' 2422 7521 1.0 9.0012-18 AA174078.1 EST-HUMAN zp18g12.sl Stratagene fetal retina 937202 Horno sapiens cDNA done IMAGE:609862 3 3718 8855 1.8 8.OOE-18 4758977 NT Horno sapiens protein tyrosine phosphatase, non-teceptor type substrate 1 (PTPNS1) mRNA W0b04.xl NCI-CGAP_Panl Homo sapiens eDNA done IMAGE:2837071 Ysimilar to 346 5527 10653 2.9 7.OOE-18 AW316976.1 EST_HUMAN gb120868 60S RIBOSOMAL PROTEIN L4 (HUMAN); NCI-CGAP_Panl Homo saplans cDNA done IMAGE:2837071 Ysimilar to 346 5527 10654 2.9 7.OOE-18 AW316976.1 EST HUMAN gb120868 60S RIDOSOMAL PROTEIN 1.4 (HUMAN); HS21C084 4338 9461 14550 1,0 7.OOE-18 AL163284.2 NT Horno sapiens chromosome 21 segment Infant brain 1I NIB Homo sapiens cDNA done IMAGE:53285 3' similar to ya49c07.sl Soares 5136 10231 15306 0.9 7.0OE-11 8 R16220.11 EST HUMAN contains L1 repetitive element., 4491 9611 1.2 6.0012-18 AL163268.2 NT Homo sapiens chromosome 21 segment HS21 C068 GAMMA-GLUTAMYLTRANSFERASE (TISSUE PROTEIN-GLUTAMINE 4641 9755 3,4 6.OOE-18 P52181 SWISSPROT TRANSGLUTAMINASE) (TGASE C) (TGC) Soares_placenta_8togweeks_2NbHP8togW Horno sapiens cDNA done [::1E62616 11418 5.0 5.OOE-18 A1280214.1 EST_HUMAN - qm6Sgl 1.xl IMAGE:1893668 Ysimilar to contains Alu repetitive element; Page 79 of 196 Table 4

F- 1 5058 10156 15232 1.8. 5.00IE-18 D61517.1 EST HUMAN HUM41 1 F0513 Clontech human fetal brain polyA+ mRNA (#6535) Homo sapiens cIDNA done GEN-41 1 F05 & 119 5318 10447 1.4 4.0OE-18 BE044076.1 EST-HUMAN ho36h04.xl NCI WAPUtl Homo sapiens cDNA clone IMAGE:3039511 Ysimilar to contains MER29---63MER29 repetitive element; 119 5318 10448 1.4 4.OOE-18 BE044076.1 EST-HUMAN ho36h04.xl NCI - CGAP - Utl Homo, sapiens cDNA done IMAGE:3039511 Ysimilar to contains 1 MER2613 MER29 repetitive element; 1846 6964 1.1 4.0OE-18 A1738592.1 EST-HUMAN M33h08.xl NCI CGAP Co16 Homo sapiens cIDNA done IMAGE:2392095 X 2154 7264 12472 1.0 4.OOE-18 006430 SWISSPROT N-ACETYLLACTOSAMINIDE BETA- 1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE (N ACETY1-GLUCOSAMINY1-TRANSFERASE) (I-BRANCHING ENZYME) (IGNT) 2154 7264 12473 1.0 4.OOE-18 Q06430 SWISSPROT N-ACETYLLACTOSAMINIDE BETA1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE (N ACETY1-GLUCOSAMINYLTRANSFERASE) (I-BRANCHING ENZYME) (IGNT) 4793 9904 14997 1.1 4.OOE-18 AF119668.1 NT Rattus norvegicus llpolysis- stimulated remnant receptor alpha' subunit mRNA, complete cds 831 5980 11141 0.8 3.OOE-18 AA814196.1 EST HUMAN ob23h11.sl NCI_CGAP_KJd5 Homo sapiens cIDNA done IMAGE:1324581 X similar to SW:RS5 - HUMAN P46782 40S RIBOSOMAL PROTEIN S5.; 914 6060 11218 0.8 3.0OE-18 BE088634.1 EST-HUMAN CMO-BT0690-210300-298- 907 BT0690 Homo sapiens cDNA 3867 9003 14103 1.2 3.OOE-18 AL163247.2 NT Homo sapiens chromosome 21 segment HS21 C047 251 5439 10566 1.0 2.OOE-18 AW836820.1 EST HUMAN QV1-LT0036-150200-070- e07 LT0036 Homo sapiens cDNA 1135 6271 16.0 2.OOE-18 BE256097.1 EST HUMAN 601114352F1 NII-I MGC 16 Homo sapiens cDNA clone-IMAGE:3355044 5' 2649 7738 12938 1.4 1.OOE-18 AL163267.2 NT Homo sapiens chromosome 21 segment HS21 C067 4318 9441 1.0 1.OOE-18 T95406.1 ES HUMAN ye43g05.rl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA done IMAGE:120536 5, similar to contains L1 repetitive element; 533 5697 10821 1.5 9.OOE-19 AA281961.1 EST-HUMAN zM 1 d06.rl NCLWAP-GC131 Homo sapiens cDNA done IMAGE:712811 Csimilar to contains MER19.t2 MER19 repetitive element; 534 5697 10821 1,1 9.00EA 9 AA281961.1 EST HUMAN ztlldO6.rl NCI CGAF_GCBI Homo saplens eDNA done IMAGE:712811 5'sImilar to contains MER19.t2 MER19 repetitive element; 1032 6172 0.5 8.OOE-19 AW974902.1 EST-HUMAN EST387007 MAGE resequences, MAGN Homo sapiens cDNA 4266 9391 14474 2.7 8.0012-19 AW902939.1 EST HUMAN QV3-NW025-100500-183- b01 NN1025 Homo saplens cDNA 2198 7307 12519 0.6 7.00IE-19 4758139 NT Homo sapiens DEAD/H (Asp-Glu-AJa- Asp/His) box polypepfide 6 (RNA helicase, 54k13) (DDX6) mRNA 3713 8851 1.0 6.00IE-19 AW852930.1 EST-HUMAN PMO-CTO248-131099-001-9 1 CTO248 Horno sapiens cDNA 4360 9482 14573 1.4 6.OOE-19 P34986 SWISSPROT OLFACTORY RECEPTOR 6 (M50).

4360 9482 14574 1.4 6.OOE-19 P34986 SWISSPROT OLFC-TORY RECEPTOR 6 (M50) 4694 9806 1.2 6.OOE-19 A.1271735.1 NT Homo sapiens Xq pseudoautosomal region; segment 112 4952 10057 15127 1.1 6.OOE-19 AL120817.1 EST HUMAN DKFZp762F1 92-rl 762 (synonym: hme12) Homo sapiens cDNA clone DKFZp762F1 92 5' 542 5705 10825 1.5 4.OOE-19 AB007970.1 NT Homo sapiens mRNA, chromosome 1 specific transcript KIAA0501 3778 8911 14007 1.7 3.OOE-19 Q28997 SWISSPROT BETA-2 ADRENERGIC RECEPTOR - 3778 8915 14008 1,7 3.OOE-19 028997 SWISSPROT BETA-2 ADRENERGIC RECEPTOR 2513 7609 12811.4 2.OOE-19 AL163201.2 NT Homo sapiens chromosome 21 segment HS21C001 2 5639 0.5 1.OOE-19 BE40861 1.1 IEST HUMAN 601304125F1 Nilmi_lVIGQ_21 Homo sapiens cDNA done IMAGE:3638310 5' Page 80 of 196 Table 4

2118 7229 12438 0.5 11,0OE-19 H30795.1 EST HUMAN yo79g07xl Soares adult brain N2b4H12155Y Homo sapiens eDNA done IMAGE: 184188 V similar to contains MER1 0 repetitive element:

2685 7772 1,0 1,0OE-119 D38044.1 NT Human gene for Ah-receptor, exon 7-9 2817 7968 5.0 1,00EAQ 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 3355 8498 13607 1,3 1.OOE-19 AA834967.1 EST HUMAN aj49bl2.sl Soares- tesfis-NI-IT Homo sapiens cDNA done IMAGE:1 393631 3'similar to contains MER37.2 MER37 repetitive element, 4626 9740 14837 1.3 8.OOE-20 H68656,1 EST-HUMAN yr87h12xl Soares fetal liver spleen INFLS Homo sapiens cIDNA done IMAGE:212327 5, 3508 8649 13759 3.9 6,OOE-20 P39188 SWISSPROT ALU SUBFAMILY J SEQUENCE CONTAMINATION WARNING ENTRY 2092 7203 12408 1.0 3.OOE-20 U03888.1 NT Human BXP21 gene 4115 9241 14327 1.7 3.OOE-20 P23273 SWISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 114 4524 9643 14733 0,9 3.OOE-20 AA037616.1 EST-HUMAN zk36b12.sli Soares_pregnanLuterus - NbHPU Homo sapiens cIDNA done IMAGE:484895 3' similar to contains Ll.t3 L1 repetitive element; 813 5963 1.5 2.OOE-20 AW303868.1 EST-HUMAN xr24e10.xl NCLCGAP-UR Homo saplens cDNA done IMAGE:2761098 Ysimilar to SW:RS5 MOUSE P97461 40S RIBOSOMAL PROTEIN SS.; 1095 6233 11384 0.7 2.OOE-20 AA516335.1 EST-HUMAN n969h09.sl NCI_CGAP_Lip2 Homo sapiens cDNA done IMAGE:940097 similar to TR:G1224066 G1224066 ORF2: FUNCTION UNKNOWN.; 1095 6233 11385 0.7 2,OOE-20 AA516335.1 EST-HUMAN ng69h09.sl NCI - C_Lip2 Homo sapiens cIDNA done IMAGE:940097 similar to TR:G1224066 G1224066 ORF2: FUNCTION UNKNOWN.; 2173 7282 12495 0.6 2.OOE-20 H68656.1 EST HUMAN yr87h112xl Soares fetal liver spleen 1NFI-S Homosapiens cIDNA clone IMAGE:2123275' 2786 5963 0.9 2.OOE-20 AW303868.1 EST HUMAN xr24e10.xl NCI-CGAP-LIM Homo sapiens cDNA done IMAGE:2761098 Ysimilar to SW:RS5_MOUSE P97461 40S RIBOSOMAL PROTEIN S5.

4866 9973 15057 4.3 2.OOE-20 028983 SWISSPROT ZONADHESIN PRECURSOR 4866 9973 15058 4.3 2,0OE-20 Q28983 SWISSPROT ZONADHESIN PRECURSOR 5090 10188 11.4 2.0012-20 5174538 NT Homc, sapiens malate dehydrogenase 1, NAD (soluble) (MDH1) mRNA 1967 7870 12283 J.9 1.0OE-20 AA281961.1 EST-HUMAN ztlld06.rl NCI - CGAP - GCBI Homo sapiens cIDNA done IMAGE:712811 5'similar to contains MER19A2 MER19 repetitive element; 4764 9875 14970 1.0 1.OOE-20 7661767 NT Homo sapiens HSPC064 protein (HSPC064), mRNA 308 5492 10621 1.0 9.OOE-21 BE165641.1 EST-HUMAN CM4-HT0481-310100-085- d07 HT0481 Homo sapiens cIDNA 2883 8034 1.1 9.0012-21 AJ003514.1 ES-r_HUMAN AJ00351 Selected chromosome 21 cIDNA library Homo sapiens cIDNA clone MP1p112-8j21 172 5365 10490 1.0 8.0OE-211 A1795842.1 EST-HUMAN RCO-HT0023-300699-001- E04 HT0023 Homo sapiens cIDNA 172 5365 10491 1.0 8.0OE-211 A1795842.1 EST HUMAN RCO-HT0023-300699-001- E04 HT0023 Horno s.plens cIDNA 2023 7136. 12335 0.8 7.OOE-21 P15800 SWISSPROT LAMININ RETA-2 CHAIN PRECURSOR (S-LAMININ) (LAMININ CHAIN B3) 2023 7136 12336 0.8 7.02-21 P15800 SWISSPROT LAMININ BETA-2 CHAIN PRECURSOR (S-LAMININ) (LAMININ CHAIN B3) 4161 __9287 5.0 7.OOE-21 AA046502..1 EST-HUMAN zk67a06.rl Soares_pregnanLuterus-NbHPLI Homo sapiens cDNA clone IMAGEA87858 5' [4014 9145 14228 0.9 6.OOE-21 BE40861 1.1 EST HUMAN 601304125F1 N1H MGC 21 Horno sapiens cDNA done IMAGE:3638310 5' 0.6 5.0OE-211 5902031 NT Homo saplens protein tyrosine phosphatase, nonreceptor type 21 (PTPN211), mRNA Page 81 of 196 Table 4

2234 7341 12553 1.1 5.0OE-211 AA928194.1 EST HUMAN om23g03.sl Soares_NFL T GBQ_SI1 Homo sapiens cDNA done IMAGE:1541908 Ysimilar to TR:002711 002711 Pii-POL-DUTPASE POILYPIR6TEIN; 4696 9806 14907 6.0 5.0012-21 4885474 NT Horno sapiens melanoma antigen, family Q 1 (MAGEC1), mRNA 5109 10205 1.0 5.0012-21 D87675.1 NT Horno sapiens DNA for amyioJd precursor protein, complete cds 1701 6824 12005 1.2 4.OOE-21 AA970713.1 EST HUMAN oo86e08.s11 NCI - CGAP- lKM5 Homo saplens cDNA clone IMAGE: 1573094 3' similar to TR:Q16530 Q16530 PMS3 MRNA;contains OFIR.M OFR repetitive element 2228 7335 12547 1.1 3.OOE-21 AL163201.2 NT Homo sapiens chromosome 21 segment HS21 C001 3041 8192 13294 4.0 3.0012-21 AJ007973.1 NT Homo sapiens LGIVID213 gene 5336 5,0 2.0OE-211 BE163247.1 EST HUMAN QV3-HT0458-170200-090-912 HT0458 Homo sapiens cDNA 919 6065 11220 0.9 2.0OE-211 ABOO7857.2 NT Homo sapiens mRNA for KIAA0397 protein, partial cds 919 6065 11221 0.9 2.0OE-211 A13007857.2 NT Homo saplens mRNA for KIAA0397 protein, partial cds 1164 6299 11454 2,6 2.OOE-21 AA206749.1 EST-HUMAN zq79bl 1A Stratagene hNT neuron (#937233) Horno sapiens cDNA clone IMAGE:647805 5' similar to WYGA1_YEAST P48234 HYPOTHETICAL 81.7 KI) PROTEIN IN MOLl-NAT2 INTERGENIC REGION, [11; 1196 6328 0.7 2.OOE-21 BE064410.1 ESTHUMAN RC4-BT0311-141 199-011 -h06 BT0311 Horno saplens cDNA 2260 7366 12575 2,6 2.OOE-21 D14547.1 NT Human DNA, SINE repetitive element 2516 7612 12814 0.8 2.0OE-211 T1 0311,1 EST HUMAN seq11487 b41-1133MA, Cot8-1-11AP-Ft Homo sapiens cIDNA done b4HB3MA-COT8-HAP-Ft259 5' 2599 7690 12893 1.0 2.OOE-21 Q28983 SWISSPROT ZONADHESIN PRECURSOR 2599 7690 12894 1.0 2.OOE-21 028983 SWISSPROT ZONADHESIN PRECURSOR 1235 6364 11526 0.6 1.OOE-21 AA557657.1 EST HUMAN n146c04.sl NCLWAP-Pr4 Homo sapiens cDNA clone IMAGE:1043718 similar to contains MER2912 MER29 repetitive element; 1381 6508 0.9 11.0OE-21 A1601264.1 EST-HUMAN ar88d12.xl Barstead colon HPLIRB7 Homo saiens cDRA done IMAGE:2152343 3' 4312 9435 14525 26 9.OOE-22 A1702438.1 EST-HUMAN tz94a03.xl NCI - CGAPKJdl 1 Homo saplens cDNA done IMAGE:2296204 3' similar to TR:Q115408 Q15408 NEUTRAL PROTEASE LARGE SUBUNIT; 933 6080 1.6 8.OOE-22 BE144748.1 EST-HUMAN CMO-HT0179-281099-076-h05 HT0179 Horno saplens cDNA 649 5807 10933 1.4 7.OOE-22 AL163246.2 NT Homo sapiens chromosome 21 segment HS21 C046 4186 9312 14393 2.3 7.OOE-22 Q61838 SWISSPROT ALPHA-2-MACROGLOSULIN PRECURSOR (ALPHA2M) 4969 10072 16145 1.2 7.OOE-22 AB008681.1 NT Homo sapiens gene for acdvih receptor type 1IB, complete cds 3584 8723 0.9 4.OOE-22 AJ271735.1 NT Homo sapiens n pseudoautosomal region; segment 112 1 943 6090 1.2 3.OOE-22 A1469679.1 EST-HUMAN 1m114h10.xl NCl-WAP-Co14 Horno saplens cDNA done IMAGE:2156611 Ysimilar to gb119593 HIGH AFFINITY INTERLEUKIN-8 RECEPTOR B (HUMAN);contains Ll.tl L1 repetitive element; 2523 7619 12820 0.7 3.0OE-22 A1859038.1 EST HUMAN YA66b04.xl NCI CGAP13m25 Homo sapiens cDNA done IMAGE:2429839 Ysimilar to SW:RJ-211 HUMAN P46778 60S RIBOSOMAL PROTEIN L21.; 3620 8759 1.5 3.OOE-22 D14718.1 NT Human chromosomal protein HMG1 related gene 4212 9336 14419 0.8 3.OOE-22 F00548.11 EST HUMAN HSB1OE082 STRATAGENE Human skeletal muscle cDNA library, cat. #936215. Homo sapiens cDNA clone 1 0E08 4212 9336 14420 0.8 3.OOE-22 F00548.11 EST_HUMAN HSB1OE082 STRATAGENE Human skeletal muscle cDNA library, cat. #936215. Homo sapiens cDNA clone 10E08 G Page 82 of 196 Table 4

In 4695 9807 14906 2.7 3.OOE-22 A1090125.1 EST HUMAN qb28c07.xl Soares_pregnant - uterus - NbHPU Homo sapiens cDNA clone IMAGE:1697580 3' similar to contains MER112.t2 MER12 repetitive element; 1910 7027 1.0 2.OOE-22 N24942.11 EST HUMAN yx73d05.sl Soares melanocyte 2NbHM Horno saelens cDNA'Cjone IMAGE:267369 3' 2476 7574 12776 1.3 2.0OE-22 P24916 SWISSPROT IMMEDIATE EARLY GENE 13 PROTEIN PRECURSOR 3373 8516 13623 4.1 2.OOE-22 8394043 NT Homo sapiens protein kinase, AMP- activated, gamma 3 non-catalyfic subunit (PRKAG3), mRNA 3587 8726 13822 0.9 2.OOE-22 R58726.1 EST HUMAN G4789 Fetal heart Homo saplens ODNA done G4789 5' end 4133 9259 14340 1.3 2.OOE-22 AW817794.1 EST-HUMAN PM1-ST0262-261 199-0011- 02 ST0262 Homo sapiens cDNA 63 5271 10393 0.8 1.0OE-22 AW886543.1 EST-HUMAN RC1-OT0083-220300-021-c07 OT0083 Homo saplens cDNA 63 5271 10394 0.8 1.0OE-22 AW886543.1 EST-HUMAN RC1-OT0083-220300-021-c07 OT0083 Homo saplens cDNA 1836 6955 12141 0.6 1.OOE-22 AW865517.1 EST-HUMAN PM4-SNO020-010400-009- h02 SNO020 Homo sapiens cDNA 2400 7943 0,5 1.0012-22 AA199812.1 EST-HUMAN zq7611 1.rl Stratagene hNT neuron (#937233) Homo sapiens eDNA clone IMAGE:647565.5' similar to SWVPRT-HUMAN P10265 RETROVIRUS-RE ED PROTEASE; 2539 7635 12835 1.0 1.OOE-22 U50871.1 NT Human familial Akhelmer's 5i- seaseksTm2) gene, complete cds 3365 8508 13616 1.7 11.0OE-22 D14547.1 NT Human DNA, SINE repetitive element 3523 8664 13768 0.8 8.OOE-23 AF198349.1 NT - Gallus galJus Dach2 protein (Dach2) mRNA, complete cds 2800 6795 11971 1.8 7.OOE-23 AA189080.1 EST-HUMAN zq45b06S1 Stratagene hNT neuron (#937233) Homo saplens cDNA done IMAGE:632627 3' similar to contains Alu repetitive element; 3387 8530 1.6 6.OOE-23 AF199333.1 NT Rattus norvegicus R151 B (Riml B) mRNA,'complete cds 4171 9297 14378 3,1 6.OOE-23 AL163249.2 NT Homo sapiens chromosome 21 segment HS21 C049 650 5808 10934 1.5 2.OOE-23 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1 gene 1126 7866 0.9 2.OOE-23 M55270.11 NT Human matrix Gla protein (MGP) gene, complete eds 2765 7849 13051 1.1 2.OOE-23 P22105 SWISSPROT TENASCIN-X PRECURSOR (TWX) (HEXABRACHION-LIKE) 2765 7849 13052 1.1 2.OOE-23 P22105 SWISSPROT TENASCIN-X PRECURSOR (TWX) (HEXABRACHION-LIKE) 3325 8469 1.5 2.OOE-23 A1201458.1 EST HUMAN qs73f111.xl NCI - CGAP-Pr28 Homc sapiens cDNA done IMAGE:1 943757 3 similar to TR:013537 013537 MER37 TRANSPOSABLE ELEMENT, COMPLETE CONSENSUS SEQUENCE.; 3655 8794 3.0 2.OOE-23 BE165980.1 EST-HUMAN MR3-HT0487-150200-113-g01 HT0487 Homo sapiens cDNA 3894 9029 14121 3.0 2.00IE-23 H59931.1 EST-HUMAN yr16a02xl Soares fetal liver spleen 1 NFLS Horno sapiens cDNA done IMAGE:f05418 5' 3894 9029 14122 3.0 2.OOE-23 H59931.1 EST-HUMAN yr16a02xl Soares fetal liver spleen WLS Homo sapiens cDNA done IMAGE:205418 5' 4428 9549 14642 1.6 11.0OE-23 A1-163252.2 NT Hamo sapiens chromosome 21 segment HS21 C052.

4657 9769 4.5 1.OOE-23 AL163210.2 NT Homo sapiens chromosome 21 segment HS21C010 540 5703 0.6 9.OOE-24 AA663213.1 EST-HUMAN ab75a08.sl Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE:852758 3' similar to TR:E19822 E19822 CA PROTEIN.; 4549 9668 14757 1.1 8.OOE-24 P23269 SWISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 13 4549 9668 14758 1.1 8.OOE-24 P23269 SWISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 13 3796 8933 1.3 7.OOE-24 AW937954.1 EST-HUMAN OVO-DT0047-170200-122-a06 DT0047 Homo sapiens cDNA 69 i 5845 0.8 -24 ABOO1421.1 NT Macaca fuscata mRNA for Tesds-Spedfic Protein Y (TSPY), complete ods 3 6.OOE Page 83 of 196 Table 4

1 1 820 6970 11127 3.5 6.OOE-24 AL163249.2 NT Homo saplens chromosome 21 segment HS21 C049 4068 9195 14278 2.1 6.OOE-24 AF199333.1 NT Rattus norvegicus RIM1 B (Riml B) mRNA, compete cds 4068 9195 14279 2.1 6.OOE-24 AF199333.1 NT Rattus norvegicus RIM1 B (Riml B) mRNA, complete ods 3889 9025 14117 7,2 5.OOE-24 AJ229043.1 NT Homo sapiens 959 kb contiq between AMLI and CBRI on chromosome 21 q22, segment 313 2298 7402 12610 0.8 2.0012-24 AA167539.1 EST HUMAN zpl 1 f09.rl tratagene fatal retina 937202 Homo sapiens cDNA clone IMAGE:609161 5' 3216 8364 13475 1.0 2.OOE-24 AA293827.1 EST-HUMAN A65b06xl Soares testis NHT Homo sapiens cIDNA clone IMAGE:727187 5' 3729 8866 1.0 2.OOE-24 AW898189.1 ESTHUMAN RC3-NNO068-090500-021-b03 NNO068 Homo sapiens cDNA 1670 6794 11970 0.8 1.0OE-24 7706340 NT Homo sapiens CGI-127 protein (LOC51646), mRNA 2638 7727 0.7 1.OOE-24 AW820194.1 EST-HUMAN QVO-ST0294-100400-185-cl 0 ST0294 Homo sapiens cDNA 2987 8139 13242 0.9 1.OOE-24 D86423.1 NT Mus musculus mRNA for HGT keratin, partial cds 4173 W-9-9 1.6 1.OOE-24 AF143313T NT Homo sapiens PTEN (PTEN) gene, exon 2 4926 10033 15107 2.3 7.OOE-25 AA483944.1 EST HUMAN ne92e1O.sl NCI - CGAP_Kidl Homo sapiens cDNA clone IMAGE91 1754 similar to contains MER112 MER1 repetitive element; 4710 9822 5.5 6.0012-25 039575 SWISSPROT DYNEIN GAMMA CHAIN, FLAGELLAR OUTER ARM 5166 10260 15331 1.0 5.OOE-25 AW838171.1 EST-HUMAN QV2-LT0051-260300A 11403 LT0051 Homo sapiens cDNA 1430 6557 11733 0.8 4.OOE-25 T98107.1 EST HUMAN ye56h04xl Soares fetal liver spleen 1NFLS,Homo sapiens cIDNA clone IMAGE:1217835' 3356 8499 2.8 4.OOE-25 AW887671.1 EST HUMAN PM3-OT0093-280200-001-g07 OT0093 Homo sapiens cDNA 3979 9111.14193 0.8 4.OOE-25 7106446 NT Mus musculus vAngless-related MIVITV Integration site 3A Mnt3a), mRNA 3979 9111 14194 0.8 4.OOE-25 7106446 NT Mus musculus wingless-related MIVITV Integration site 3A (Wnt3a), mRNA 4221 9345 3.0 4.OOE-25 BE170957.1 EST HUM-AN QV3-HT0543-140400-149-el 1 HT0543 Homo saplens cDNA 3278 8423 13531 2.7 3.OOE-25 8923321 NT Homo sapiens hypothetical protein FLJ20344 (FLJ20344), mRNA 3278 8423 13532 2.7 3.OOE-25 8923321 NT Homo sapiens hypothefical protein FLJ20344 (FI-J20344), mRNA 4798 9909 15002 0.9 3.OOE-25 P29622 SWISSPROT KALLISTATIN PRECURSOR (KALLIKREIN INHIBITOR) (PROTEASE INHIBITOR 4) 1325 6454 11620 1.2 2.OOE-25 5032158 NT Homo sapiens transducin (beta)- like 1 (T131-1) mRNA 2363 7465 12670 1,3 2.OOE-25 AW954060.1 EST HUMAN EST366130 MAGE reseque2ces, MAGC Homo sapiens cDNA 2801 7592 12795 1.6 2.OOE-25 P17008 SWISSPROT 40S RIBOSOMAL PROTEIN S16 4096 9222 14305 1,9 2.OOE-25 P17008 SWISSPROT 40S RIBOSOMAL PROTEIN S16 4096 9222 14306 1.9 2.OOE-25 P17008 SWISSPROT 40S RIBOSOMAL PROTEIN S16 361 5539 10670 0.6 1.OOE-25 AL040229.1 EST-HUMAN DKFZp434H0313-rl 434 (synonym: htes3) Homo saplens eDNA clone DKFZp4341-10313 5' 2384 7486 12688 2.8 1.OOE-25 Q06055 SWISSPROT ATP SYNTHASE LIPID-BINDING PROTEIN P2 PRECURSOR (ATPASE PROTEIN 9) (SUBUNIT C) 4753 9864 14957 2.7 1.OOE-25 BE162737.1 EST HUMAN PM1-HT0454-080100-002- h09 HT0454 Homo sapiens cDNA 2433 7532 12738 0.7 9.OOE-26 AL163218.2 NT Homo sapiens chromosome 21 segment HS21 C01 8 15596686 11853 0.5 7.OOE-26 AF003528.1 NT Homo sapiens X-linked anhidrolfic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 3898 9033 14126 1.4 7.OOE-26 X8921 1.1 NT H.sapiens DNA for endogenous retrovirai like element 4067 9 2.0 7.OOE-26 AW340153.1 EST-HUMAN W02e12.xl Soares NFL T G8C S1 Homo sapiens cIDNA clone IMAGE:2908366 3' c) Page 84 of 196 Table 4

Homo sapiens chromosome 9 duplication of the T cell receptor beta locus and trypsinogen 2178 7287 12499 0.9 6.OOE-26 AF029308.1 NT gene families zq52h04xl Stratagene neuroepithellum (#937231) Homo sapiens cDNA done 3302 8447 13557 1.4 6.OOE-26 AA206131.1 EST HUMAN IMAGE:645271 V as38h08.xl Barstead aorta HPI-RB6 Homo sapiens cDNA clone IMAGE:2319519 3'sImilar to 1160 6295 11450 1.1 5.0012-26 A1708235.1 EST---HUMAN WPY49C12.11 CE03371; HPLIRB6 Homo sapiens cDNA clone IMAGE:2319519 Ysimilar to as38h08.xl Barstead aorta 1160 6295 11451 1.1 5.OOE-26 A1706235.1 EST HUMAN WRF49C12.11 CE03371; sEcificity factor mRNA, complete cds 2692 7779 __12976 1.8 5.00IE-26 U37012.1 NT Human cleavage and polyadenylaflon 12 ek 11 Homo sapiens cDNA V end 1525 6d-5-2 1.5 4.OOE-26 AA329548.1 EST HUMAN EST33446 Embryo, htes3) Homo sapiens cDNA done DKFZp4341066.5' 1958 7075 12272 0.5 3.OOE-26 AL045855.2 EST HUMAN DKFZp4341066 rl 44 (synonym:

NT2RAMI 937234 Homo sapiens cDNA done zn30d08xl Stratagene heuroepfthellum IMAGE:548943 5'slmilar to qb:M14338 VITAMIN K-DEPENDENT PROTEIN S 1987 7102 0.9 3.OOE-26 AA115895.1 EST HUMAN PRECURSOR(HUMAN); (#937204) Homo sapiens cDNA done IMAGE:588427 5' similar zo30f10xl Stratagene colon 3712 8850 13940 1.2 3.OOE-26 AA1 52464.1 EST HUMAN to TR:G695374 G695374 THYROID RECEPTOR INTERACTOR; colon (#937204) Homo sapiens cDNA clone IMAGE:588427 5' similar 3712 8850 13941 __1.2 3.OOE-26 AA152464.1 EST HUMAN zo30f10xl Stratagene 666 5822 10950 1.9 2.OOE-26 AL163282.2 NT to TR:G695374 G695374 THYROID RECEPTOR INTERACTOR; Homo sapiens chromosome 21 segment HS21 C082 1825 6944 13456 1.2 2.OOE-26 AL038099.2 EST HUMAN DKFZp5661-17l---pl 566 (synonym: hfKd2) Homo sapiens cDNA clone DKFZp5661-171 3' 3193 8342 4.3 2.OOE-26 X86694.1 NT M.musculus mRNA for astrocytic phosphoprotein, PEA-1 5 132 5328 10459 0.8 1.OOE-26 BE170371.1 ESTHUMAN OV4-HT0538-020300-123-a02 HT0538 Homo sapions eDNA 2001 2116 12318 1.4 1.OOE-26 AL-039363.2 EST_HUMAN DKFZp434H19100 434 (synonym: htes3) Homo sapiens eDNA clone DKFZp4341-11910 5, glyceraidehyde-3-phosphate dehydrogenase (GAPD). mRNA 2654 7743 6.1 1.OOE-26 7669491 NT Homo sapiens NCI-C_Lul g Homo sapiens cDNA done IMAGE:2406150 Y similar to v449c04.xl 5218 10319 1.1 8.OOE-27 A1831462.1 EST HUMAN contains THR12 THR repetitive element; 545 5708 1.4 8.OOE-27 AL163227.2 NT Homo sapiens chromosome 21 segment HS21C027 fetal brain 00004 Homo sapiens cDNA done IMAGE:2783295 3, au87h0B.xl Schnelder 1395 6522 11690 6.8 8.OOE-27 AW162737.1 EST HUMAN similar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN)., au87h08.xl Schnelder fetal brain 00004 Homo saplens cDNA clone IMAGE:2783295 3' 1395 6522 11691 6.8 8.OOE-27 AW162737.1 EST HUMAN similar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN); PM2-SNO01P2-20300-002-a07 SNO018 Horno saplens cDNA 2121 7231 12441 1.0 8.OOE-27 AW864776.1 EST-HUMAN LIVER ISOFORM T2 (ADPIATP TRANSLOCASE 3) ADP.ATP CARRIER PROTEIN, 3148 8297 13406 3,3 8.OOE-27 P12236 SWISSPROT (ADENINE NUCLEOTIDE TRANSLOCATOR 3) (ANT 3) 3309 8454 13567 0.9 8.OOE-27 AF181897.1 NT Homo saplens WRN (WRN) gene, completwcds 668 5824 1.2 7.OOE-27 Z70664.1 NT Human endogenous retroviral element HC2 Homo saplens cDNA clone IMAGE:2975879 3' similar h1511h12.xl Soares_ISIFL-T-GBQ_SI1 5023 10124 12314 2.1 7.OOE-27 AW629172.1 EST-HUMAN to TR:076040 076040 ORF2: FUNCTION UNKNOWN.; L__1" 7111 2,0 3.OOE-27 X60658.1 NT R.rattus RYA3 mRNA for a potential ligand-binding protein Page 85 of 196 1 Table 4

4174 9300 14381 1.3 3.OOE-27 BE071924.1 EST HUMAN PMO-BT0527-0901 00-001.d 11 BT0527 Homo saplens cDNA 36 52Z5 10352 8.0 2.OOE-27 AF054187.1 NT Homo sapiens alpha NAC mRNA, complete cds 1853 6971 6.7 2.OOE-27 AA565345.1 EST HUMAN nkOlblO.sl NCI-CGAP - Pr11 Homo sapiens cDNA done IMAGE: 1000699 similar to gb:M1 7886 60S ACIDIC RIBOSOMAL PROTEIN P1 (HUMAN); 3071 8222 10.3 2.OOE-27 AW629172.1 EST-HUMAN W511112.xl Soares-INIFL-T- GI3C-S1 Homo sapiens cDNA done IMAGE:2975879 Ysimilar to TR:076040 076040 ORF2: FUNCTION UNKNOWN.; 3187 8336 13448 1.4 2.OOE-27 AF1 11167.2 NT Homo sapiens jun dimerization protein gene, partial cds; cfos gene, cornple7e cds; and unknown gene 3187 8336 13449 1.4 2.OOE-27 AF1 11167.2 NT Homo sapiens jun dimerization protein gene, partial cds; cfos gene, complete cds; and unknown gene 3944 9077 14159 1.1 2.00E-27AF000368.1 NT Rattus norvegicus voltage- gated sodium channel mRNA, complete cds 429 5596 0.5 1.OOE-27 AL163246.2 NT Homo saplens chromosome 21 segment HS21C046 980 6125 11281 1.4 1.OOE-27 AB026898.1 NT Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 112 (DI-EC1, ORCTI-3, ORCTL4 genes, complete cds) 1438 6565 11742 2.8 1.OOE-27 AF029308.1 NT Homo sapiens chromosome 9 duplication of the T cell receptor beta locus and trypsinogen gene families 136 5331 0.8 9.OOE-28 BE348399.1 EST HUMAN hw17cl 1.xl NCI-CGAP_Lu24 Homo sapiens cDNA done IMAGE:3183188 Ysimilar to TR:Q07314 Q07314 SECRETED NEUREXIN Ill-ALPHA-C PRECURSOR. [3) TR:Q07280 TR:Q07313; 1140 6276 11428 1.1 9.OOE-28 W23234.1 EST-HUMAN 81 B2 Human refina cIDNA Tsp5091-cleaved sublibrary Homo sapiens cDNA not directional 5118 10214 15292 1.2 9.OOE-28 A15901 15.1 EST-HUMAN to12b09.xl NCI-CGAPUt2 Homo sapiens cDNA done IMAGE:2178809 Ysimilar to contains OFR.tl OFR repetitive element; 5118 10214 15293 1.2 9.OOE-28 A1590115.1 EST HUMAN to12b09.xl NCI---WAP W2 Homo sapiens cDNA done IMAGE:2178809 3'sImilar to contains OFR.tl OFR;peuuve element; 3987 9119 14202 1.3 6.OOE-28 A13020673.1 NT Homo sapiens mRNA for KIAA0866 protein, complete ods 3987 9119 14203 1.3 6.OOE-28 A13020673.1 NT Homo sapiens mRNA for KIAA0866 protein, complete ods 315 5499 1.1 5.OOE-28 A1921003.1 EST HUMAN wo18c07.xl NCI-CGAP-Panl Homo saplens cIDNA clone IMAGE:2455692 3'slmilar to contains THR.bl THR repetitive element; 3927 9061 14148 1.9 5.OOE-28 R79762.1 EST HUMAN Y189f10xl Soares placenta Nb2HP Homo sapiens cDNA done IMAGE:146443 5' 2584 7677 12879 0.5 4.OOE-28 AW195066.1 EST HUMAN xn33c09.xl NCI - CGAP- Kidl 1 Homo saplens cDNA doni IMAGE:2695504 Ysimilar to SW:GG95 HUMAN Q08379 GOLGIN-95.; 3070 8221 13320 3.4 4.OOE-28 BE409100.1--- ESTHUMAN 601300703F1 N1H MGC 21 Homo sapiens cDNA clone IMAGE:3635305 5' 1262 6391 0.6 3.OOE-28 AF155382.1 NT Homo sapiens metalloprotease-like, disintegrin-like, cysteine-flch protein 2 epsilon (ADAM22) mRNA, complete cds 83 5289 10416 2.2 2.OOE-28 BE062167.1 EST HUMAN RC1-13T0254-220300-019- c05 BT0254 Homo sapiens cDNA 1149 6284 11437 3.4 2.OOE-28 Y1 1107.3 NT Homo sapiens ITGB4 gene for Integrin beta 4 subunit, exons 3-41 7526 12732 0.8 2.OOE-28 A1348634.1 EST-HUMAN qo35b06.xl NCI-CGAP LuS Homo sapiens cDNA clone IMAGE:1910483 Ysimilar to contains 1-112 L1 repejuve element; 6590 11765 0.9 1.OOE-28 D38044,1 NT _ Human gene for Ah-receptor, exon 7-9 L' Page 86 of 196 Table 4

2650 7739 12939 1.0 1.OOE-28 AF000995.1 NT Homo, sapiens ubiquitous.TPR motif, Y Isotom (UTY) mRNA, alternative transcript 2, complete cds 1584 6710 11881 0.9 7.OOE-29 AW966447.1 EST-,HUMAN EST378521 MAGE resequences, MAGi Homo saplens cDNA 580 5740 10857 2.2 6.OOE-29 A1936748.1 EST HUMAN wp69b01.xl NCI - CGAP - 13m25 Homo sapiens cDNA clone IMAGE:2466985 3' similar to TR:015475 015475 UNNAMED HERV-H PROTEIN xontains LTR7.bl LTR7 repetitive element; 4928 10035 1.0 5.OOE-29 AL1 63203.2 NT Homo sapiens chromosome 21 segment HS21C003 3194 8343 1,7 4.0012-29 A1752367,1 EST_HUMAN cn15c02.xl Normal Human Trabecular Bone Cells Homo sapiens cIDNA clone NHTI3C w15c02 random 4317 9440 14529 1.5 3.0012-29 AB042297.1 NT Homo sapiens PTS gene for 6- pyruvoyitetrahydroptedn synthase, complete cds 484 5649 10772 1.4 2.OOE-29 AF084869.1 NT Homo, sapiens envelope protein RIC-6 (eny) gene, complete cds 484 5649 10773 1.4 2.OOE-29 AF084869.1 NT Homo sapiens envelope protein RIC-6 (env) gene, complete cds 1516 6643 11810 2,2 2.OOE-29 A1963604.1 EST HUMAN wr65dl 0.xl NCI CGAP Utl Homo sapiens cDNA done IMAGE:2492563 Ysimilar to TR:015546 01j546 HIE-RV-E ENVELOPE GLYCOPROTEIN; 1516 6643 11811 2.2 2.OOE-29 A1963604.1 EST-HUMAN wr65d10.xl NCI-CGAP-Utl Homo sapiens cDNA clone IMME:2492563 Ysimilar to TR:01 5546 015546 HERV-E ENVELOPE GLYCOPROTEIN; 1602 6729 11900 0.5 2.OOE-29 AW962209.1 EST HUMAN EST374282 MAGE resequences, MAGG Horno sapiens cIDNA 1602 6729 11901 0.5 2.OOE-29 AW962209.1 EST HUMAN EST374282 MAGE reseque2ces, MAGG Homo sapiens cIDNA 4182 9308 14387 2.0 2.OOE-29 AL-163268.2 NT Homo sapiens chromosome 21 segment HS21C068 1499 6625 1.2 7.OOE-30 BE091 133.1 EST - HUMAN PM4-BT0724-1 MOO-004-dl 1 BT0724 Homo sapiens cDNA 1549 6676 1.0 6.OOE-30 X51755.1 NT Human lambda-immunoglobulin constant region complex (germline) 1736 6859 12037 1.3 6.OOE-30 D25303.1 NT Human mRNA for Integrin alpha subunit, complete. cds 3153 8302 13410 2.4 6.OOE-30 BE008026.1 EST-HUMAN QVO-BN0147-290400-214- f12 BN0147 Homo sapiens cIDNA 3931 9065 14152 31.2 5.00IE-30 A1399992.1 EST-HUMAN tg92g03.xl NCI_PGAP- CLI-1 Homo, saplens cDNA done IMAGE:2116276 3' similar to contains Alu repetitive element; 2096 7207 12412 0.6 4.OOE-30 AW937471.1 EST HUMAN QV3-DT0043-090200-080c06 DT004314omo sapiens c1DNA 2096 7207 12413 0.6 4.OOE-30 AW937471.1 EST-HUMAN OV3-DT0043-090200-080- W6 DT0043 Horno sapiens cIDNA 1134 6270 0.6 3.OOE-30 A1338551.1 EST HUMAN qq93c05.xl Soare5_totaLfetiis- Nb2HF8 9w Horno sapiens cIDNA done IMAGE: 1938920 3' similar to contains MER2912 MER29 repetitive element; 3697 8836 13927 0.9 3.OOE-30 AF128893,1 NT Homo sapiens telomerase reverse transcriptase (TERT) gene, exons 1-6 659 5815 10943 1.2 2.OOE-30 AW857315.1 EST-HUMAN CMO-CT0307-310100-158- 1h03 CT0307 Homo, sapiens cDNA 1068 6206 0.8 2.OOE-30 F08688.1 EST HUMAN HSC23F051 normalized infant brain cDNA Homo sapiens cDNA done c-23f05 1464 6591 11766 2.1 2.OOE-30 BE175877.1 EST-HUMAN RC5-HT0582-110400-013- HO8 HT0582 Homo sapiens cDNA 28B9 8040 13141 6.4 2.OOE-30 AF1 14156.1 NT Homo saplens Y-linked Anc finger protein (ZFY) gene, complete cds 3721 8858 13951 2.1 2.0012-30 AW206581.1 EST HUMAN Ul-H-Bil-afo-c,12-0-Ul. si NCI_PGAPSuW Homo sapiens cDNA done IMAGE:2722558 3 4668 9780 14878 1.7 2.OOE-30 BE298945.1 EST-HUMAN 601119860F1 NIH MGC 17 Homo sapiens cDNA done IMAGE:3029.438 5' 4668 9780 14879 1.7 2.OOE-30 BE298945.1 EST HUMAN 601119860F1 N1H MGQ_17 Horno sapiens cDNA done IMAGE.3029438 5' 284 5471 10601 3.5 1.0OE-30 C18939.11 EST HUMAN Cl 8939 Human placenta cDNA (TFujiwara) Homo sapiens GDNA done GEN-570C01 5' J7 5691 10813 0.6 1.0OE-30 AW468897.1 EST-HUMAN W30b04.xl Soares_NFLT_GBC- Sl Horno sapiens cDNA done IMAGE:2910991 Ysimilar to contains MERIA3 MER1 MER1 repetitive element; Page 87 of 196 Table 4

1 699 5854 10989 1.0 1.OOE-30 AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 2165 7274 12485 1.2 1.OOE-30 AA664377.1 EST HUMAN ac77b08.sl Stratagene lung (#937210) Horno saplens cDNA done IMAGE:868599 3' 2973 8124 13231 1,0 1.0OE-30 5803091 NT Homo sapiens methlonine aminopepfidase,. eIF-2-associated p67 (MNPEP), mRNA 3015 8167 13268 0.9 1.OOE-30 AA315045.1 EST. HUMAN EST186868 HCC cell line (matastasis to liver In mouse) 11 Homo sapiens cIDNA Vend 1060 6199 11352 0.6 8.OGE-31 8923389 NT Homo saplens hypothetical protein FLJ20420 (FLJ20420), mRNA 2362 7464 1.9 8.OOE-31 AL1 63208.2 NT Homo sapiens chromosome 21 segment HS21 C008 694 5849 0,6 7.OOE-31 AA372637.1 EST-HUMAN ESTB4555 Colon adenocardnoma IV Homo sapiens eDNA Wend 2633 7722 12924 0.6 7.OOE-31 BE326517.1 EST - HUMAN hwO5al 1.xl NCI- CGAP_Lu24 Homo sapiens cDNA done IMAGE:3182012 3' 2633 7722 12925 0.6 7.OOE-31 BE326517.1 EST-HUMAN hwO5al 1.xl NCI---WA12 Lu24 Homo saplens cDNA done IMAGE:3182012 X 3198 8347 13460 4,5 6.OOE-31 AW881551.1 EST-HUMAN RC4-OT0037-150200-011- 908 OT0037 Homo sapiens cDNA 3198 8347 13461 4.5 6.OOE-31 AW881551.1 EST-HUMAN RC4-OT0037-11 ' 50200- 011-908 OT0037 Homo saplens cDNA 3625 8764 2.8 6.OOE-31 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 3827 8964 14063 1.1 6.OOE-31 AW067805.1 EST-HUMAN cnl9d07.xl Normal Human Trabecular Bone Cells Horno saplens cDNA clone NHTBC-cnl9d07 random 5379 10505 1.0 5.OOE-31 M60694.1 NT Homo sapiens type 1 DNA topolsomerase gene, exon 8 5379 10506 1.0 5.OOE-31 M60694.1 NT Horno sapiens type 1 DNA topolsomerase gene, exon 8 5026 10127 15200 1.7 5.OOE-31 8924243 NT Homo sapiens hypothefical protein SIRP-b2 (SIRP-b2), mRNA 5026 10127 15201 1.7 5.OOE-31 8924243 NT Homo sapiens hypothetical protein SIRP-152 (SIRP-b2), mRNA 581 5741 1.3 4.0012-31 AJ271735.1 NT Homo saplens Xq pseudoautosomal region; segment 112 1594 6720 11889 1.0 4.OOE-31 Q10473 SWISSPROT POLYPEPTIDE N- ACETYLGALACTOSAMINYLTRANSFERASE (PROTEIN-UDP ACETYLGALACTOSAMINYLTRANSFERASE) (UDP-GALNAC:POLYPEPTIDE, N ACETYLGALACTOSAMI.NYLTRANSFERASE) (GAI-NAC-T1) 1775 6896 0.9 4.OOE-31 AL163280,2 NT Homo saplens chromosome 21 segmeR HS21C080 2759 7843 1.4 4.OOE-31 5730038 NT Horno saplens SET domain and mariner transposase fusion gene (SETMAR) mRNA 2550 7645 12846 0.5 3.OOE-31 6005871 NT Homo saplens SEC63, endoblasmic reticulum translocon component (S. cerevislae) like (SEC63L), mRNA 3055 8206 1.1 3.OOE-31 4505752 NT Homo sapiens phosphoglycerate mulase 1 (brain) (PGAM1) mRNA 4303 9427 14517 1.4 3.OOE-31 A1936829.1 EST-HUMAN wp70a02.xl NCl-WAP-Bm25 Horno sapiens cDNA done iMAGE:2467082 Ysimilar to TR:060794 060794 DJ393P12.2; 1875 6993 12179 0.7 2.OOE-31 AW838171.1 EST HUMAN OV2-LT0051-260300-1 11403 LT0051 Homo sapiens cIDNA 2167 7276 12487 1.3 2.OOE-31 A1393388.1 EST-HUMAN tg44g05.xl Soares NFL T GBC S1 Homo saplens cIDNA done IMAGE:2111672 3' 2292 7396 12606 0.8 2.OOE-31 AL1 19245,1 EST-HUMAN DKFZp761G151_rl 761 (synonym: hamy2) Homo sapiens cDNA done DKI7Zp761G1513 5' 2391 7491 12696 1.6 2.0OE-311 AA458824.1 EST HUMAN. aa88fl 1.sl Stratagene fetal retina 937202 Homo, sapiens cIDNA done IMAGE:838413 3' similar to contains THR.t2 THR repetitive element; 16 5224 10326 2.8 1.0OE-31 U93163.1 NT Homo saplens MAGE-B2 (MAGE-B2), MAGE-B3 (MAGE-B3), MAGE-B4 (MAGE-B4), and MAGE-Bl (MAGE-BI) genes, complete cds 1640 6766 11935 0.7 1.OOE-31 095371 SWISSPROT OLFACTORY RECEPTOR 2C1 1640 6766 11936 "6.7 1.OOE-31 095371 SWISSPROT OLFACTORY RECEPTOR 2C1 16491 6766 11937 0.7 1.0OE-31 095371 SWISSPROT OLFACTORY RECEPTOR 2C1 Page 88 of 196 Table 4

4535 9654 14741 1.0 1.0OE-311 AL134376.1 EST. HUMAN DKFZp547B23_rl 547 (synonym: hfbrl) Horno sapiens cIDNA clone DKIFZp5478235 5' 4535 9W 14742 1.0 1.OOE-31 AL134376.1 EST-HUMAN DKIFZp547B235 ri 547 (synonym: hfbrl) Homo sapiens cIDNA done DKFZp54713235 W 2835 7986 1.0 9.OOE-32 U50871,1 NT Human familial Akhelmer's disease (STM2) gene, complete cds 2- 0- 3 27145 12347 1.2 8.OOE-32 A1056770.1 EST-HUMAN oz115a09.xl Soares_fetaLlive_spicen-1 NFL$_Sl Homo saplens cDNA clone IMAGE:1675384 3' 4754 9865 14958 1.2 7.OOE-32 P52591 SWISSPROT NUCLEAR ENVELOPE PORE MEMBRANE PROTEIN POM 121 (PORE MEMBRANE PROTEIN OF 121 KD) (P145) 2702 7788 12988 0.9 6.OOE-32 A1478104.1 EST HUMAN tm34al 0.xl NCI_PGAP- Kidl 1 Homo sapiens cDNA done IMAGE:2159994 Ysimilar to contains MER29J3 MER29 repetitive element; 1018 6158 11311 10.0 5.OOE-32 AF1 16627.1 NT Homo sapiens PROI 181 mRNA, complete cds 915 6061 0.6 4.OOE-32' AL163246.2 NT Homo sapiens chromosome 21 segment HS21C046 448 5614 10745 1.2 3.OOE-32 Y17293.1 NT Homo sapiens FLI-1 gene, partial 4783 9893 14986 0.9 2.OOE-32 BE296613.1 EST-HUMAN 601173631F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE:3529159 V 3435 8577 5.0 9.OOE-33 BE327112.1 ES'-HUMAN Inw07c05,x11 INICI-C_Lu24 Homo sapiens cDNA done IMAGE:3182216 3'similar to TR:088539 088539 WW DOMAIN BINDING PROTEIN 1 l.; 54 5263 10382 0.7 7.0OE-33 5031736 NT Homo sapiens short-chain alcohol dehydrogenase family member (HEP27) mIRNA 54 5263 10383 0.7 7.OOE-33 5031736 NT Homo saRiens short-chain alcohol dehydrogenase family member (HEP27) mRNA 2115 7226 12434 1.0 7.OOE-33 A1590115.1 EST-HUMAN to12b09.xl NCI_CGAF_Ut2 Homo sapiens cDNA done IMAGE:2178809 Ysimilar to contains OFRAI OFR iepedfive element; 3204 8352 12.9 7.OOE-33 AW971307.1 EST---HUMAN EST383396 MAGE resequencts, MAGIL Horno sapiens c 3676 8815 0.9 6.OOE-33 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 1856 6974 12158 1.4 5.OOE-33 4507208 NT Horno sapiens spermidine synthase (SIRM) mRNA 1856 6974 12159 -1.4 5.OOE-33 4507208 NT Homo sapiens spermidine synthase (SIRM) mRNA 2226 7333 1.3 5.OOE-33 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 1112 6249 3.3 4.OOE-33 AL1 63207,2 N.T Homo sapiens chromosome 21 segment HS21C007 2078 7190 12393 0.6 4.OOE-33 4758987 NT Horno sapiens RABI, member RAS oncogen family (RAB1) mRNA 2370 7472 2.7 4.OOE-33 AA626621.1 EST HUMAN ab51 bl 1xl Stratagene lung carcinoma 937218 Homo sapiens cDNA done IMAGE:844317 5 similar to contains Ak repetitive element;contalns MER2812 MER28 repetitive element; 2498 7595 12797 1.4 4.OOE-33 AL163210.2 NT Homo sapJens chromosome 21 segment HS21 C01 0 4382 9504 14589 1.6 4,OOE-33 AW293349.1 EST-HUMAN Ul-H-B12-ahl-c-03-0-Ul. sl NCI_PGAP-Sub4 Homo sapiens cDNA done IMAGE:2727149 3' 1073 6211 1.5 3.OOE-33 BE350127.1 EST HUMAN ht09901.xl NCI_PGAP_K1d13 Homo sapiens cDNA done IMAGE:3146256 3' similar to contains MER2913 MER29 repetitive element., 1074 6211 1.2 3.OOE-33 BE350127.1 EST-HUMAN ht09901.xl NCI_PGAP K1d13 Homo sapiens ciDNA clone IMAGE:3146256 3'similar to contains MER2913 MiR29 repetitive element; 4761 9872 14967 3.1 3.OOE-33 AB035346.1 NT Homo saplens TCL6 gene, exon 12 5225 0.8 2,OOE-33 All 60189.1 ESI HUMAN qb67g03 xl Soares_fetaLhear_NbHHI9W Homo sapiens cDNA done IMAGE:1705204 3' similar to contains OFR.tl OFR repetitive element; Page 89 of 196 Table 4

4322 9445 4.3 2.OOE-33 BE159039.1 EST-HUMAN MRO-HT0405-160300-202-d08 HT0405 Homo sapiens cDNA 4911 10018 15097 5.1 2.OOE-33 AA626683.1 EST-HUMAN ab51g11 1.rl Stratagene lung carcinoma 937218 Homo sapiens cDNA clone IMAGE:844388 5' similar to gb:X00734 cdsl TOBULIN BETA-5 CHAIN (HUMAN); 8 5216 1.4 1.OOE-33 AF003528,1 NT Homo sapiens X-linked anhidrolfic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 1427 6554 11730 0.7 7.OOE-34 T70845.1 EST-HUMAN yd15e05xl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE:108320 V 463 5628 10757 1.5 6.OOE-34 U10991.1 NT Human G2 protein mRNA, partial cds 463 5628 10758 1.5 6.OOE-34 U10991.1 NT Human G2 protein mRNA, partial cds 1837 6956 0.8 5.OOE-34 7706500 NT Homo sapiens Npw38-binding protein NpwBP (LOC51729), mRNA 4363 9485 1.1 5.OOE-34 AA316404.1 EST-HUMAN EST188123 HCC cell line (matastasis to liver in mouse) 11 Horno sapiens cDNA 5' end similar to similar to SK133 4993 10096 15163 4.0 5.OOE-34 U30883.1 NT Human splicing factor SRp55-1 (SRp-55) mRNA, complete cds 1953 7070 12267 0.8 4.OOE-34 A1804667.1 EST-HUMAN tt94c06.xl NCI CGAP Pr28 Homo sapiens cDNA done IMAGE:2249194 3' 2687 7774 12973 1.0 4.OOE-34 8922807 NT Horno sapiens hypothetical protein FLJ10989 (FL.110989), mRNA 1488 6615 11786 3.1 1.0OE-34 P12236 SWISSPROT AD12,ATP CARRIER PROTEIN, LIVER ISOFORM T2 (ADPIATP TRANSLOCASE 3) (ADENINE NUCLEOTIDE TRANSLOCATOR 3) (ANT 3) 3621 8760 13852 1.5 1.OOE-34 AF003528.1 NT Homo sapiens X-linked anhidrolfic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat r eglons 4381 9503 4.0 1.OOE-34 BE071414.1 EST HUMAN RC2-BT0506-240400-016-h08 BT0506 Homo sapiens cIDNA 4920 10027 0.9 11.0OE-34 AW845706.1 EST HUMAN MRO.CTO068-280999-002-dl 1 CTO068 Honio sapiens cDNA 3594 8733 13828 1.3 9.OOE-35 AW663302.1 EST HUMAN hh77b06.yll NCI_WAP_GU1 Homo sapiens cDNA clone IMAGE:2968787 5 219 5411 8.3 8.OOE-35 6031190 NT Homo sapiens prohibifin (PHB) mRNA 1391 6518 11686 1.3 6.OOE-35 AA7571 15.1 EST-HUMAN ah53h03.sl Soares testis NHT Homo saplens cDNA clone 1309397 3, 1925 7042 12231 0.6 6.OOE-35 6005975 NT Homo sapiens zinc finger r-otein 208 (ZNF208), rnRNA 1681 6805 11986 0.8 5.OOE-35 X63392.1 NT H.sapiens Immunoglobulin kappa fight chain variable region L14 4311 9434 14524 2.2 5.OOE-35 AF023268.1 NT Homo sapiens clk2 kinase (CLK2), propinl, cotel, glucocerebrosidase (GBA), and metaxin genes, complete cds; metaxin pseudogene and glucocerebrosidase pseudogene; and thrombospondW (TH13S3) gene, partial cds 1415 6543 11718 5.4 4.OOE-35 BE257907.1 EST-HUMAN 801109719F1 N1H MGQ_16 Homo sapiens cDNA done IMAGE:3350405 S' 1776 6897 12084 1.9 4.OOE-35 H91 193.1 EST HUMAN yu98a07.rl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE:241236 5' similar to contains PTR5 repetitive element; 5042 10142 1.3 4.OOE-35 BE409102.1 EST HUMAN 601300705F1 NIH MGC - 21 Homo sapiens cDNA done ltUGE:3635401 5' 1561 6688 11856 3.5 3.OOE-35 BE2681827 EST HUMAN 601125260F1 NIH MGC 8 Homo sapiens cDNA done IMAGE:3345063 5' 2283 7389 0.9 3.OOE-35 AF224492.1 NT Homo sapiens phosphollpid scramblase 1 gene, complete cds 103 7881 10434 0.6 2.OOE-35 N88965.1 ESI_HUMAN K6932F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA done K6932 5, similar to REPETITIVE ELEMENT 1171 6305 11460 1.3 2.OOE-35'T11909.1 EST-HUMAN A971 F Heart Homo saplens cDNA done A971 1203 6335 11491 0.7 2.OOE-35 6912459 NT Homo saplens Grb2-associated binder 2 (K1AA0571), mRNA 2171 7280 12493 1.9 2.0012-35 6912459 NT orno saplens Grb2-associated binder 2 (K1AA0571), mRNA __M2 2 8417 13523.1.0 2.OOE-35 6912459 NT Homo sapiens Grb2-associated binder 2 (K1AA0571), mRNA Page 90 of 196 Table 4

3272 8417 13524 1.0 2.OOE-35 6912459 NT Homo sapiens Grb2-associated binder 2 (K1AA0571), mRNA 3511 8652 0.9 2.OOE-35 AB020702.1 NT Horno saplens mRNA for KIAA0895 protein, partial cds 3835 8972 14071 1.1 2.OOE-35 BE247575.1 N TCBAP2E4328 Pedlatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC pro,Iect--TCBA Homo sapiens cDNA done TCBAP4328 3835 8972 14072 1.1 2.OOE-35 BE247575.1 EST HUMAN TCBAP2E4328 Pedlatric pre-B cell acute lymphoblastic leukernia Baytor-HGSC project=TCBA Horno sapiens eDNA done TCBAP4328 4570 9688 2.6 2.0OE-35 H49239.1 EST HUMAN yql9al2.rl Soares fetal liver spleen 1NFI-S Homo sapiens cDNA done IMAGE:274079 W 5250 10360 1.5 1.0OE-35 AA6311949.1 EST HUMAN Wcl 6 Regional genomic DNA specific cDNA library Homo sapiens eDNA done CR1 2-1 5250 10361 1,5 1.OOE-35 AA631949.1 EST-HUMAN Wcl 6 Regional genomic DNA specific cDNA library Homo sapiens cDNA done CR1 2-1 735 5889 11032 15.0 11.0OE-35 AW389473.1 EST-HUMAN IL2-ST0162-131099-006- d12 ST0162 Homo sapiens cDNA 735 5889 11033 15.0 1.OOE-35 AW389473.1 EST HUMAN IL2-ST0162-131099-006d12 ST0162 Homo sapiens cDNA - 891 6039 1.2 1.0OE-35 T87947.1 EST-HUMAN yd93a01xl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA done IMAGE:1 15752 5' similar to SP:A44282 A44282 RETROVIRUS-RELATED OL POLYPROTEIN HUMAN; 2495 7591 12794 0.8 1.OOE-35 7705994 NT Homo sapiens hypothetical protein (LOC51233), mRNA ' 2740 7824 13028 1,1 1.OOE-35 BE350127.1 EST-HUMAN ht09g01.xl NCI- CGAi_Kidl 3 Homo sapiens cDNA done IMAGE:3146256 Ysimilar to contains MER2913 MER29 repetitive element; 2740 7824 '13029 1.1 1.OOE-35 BE350127.1 EST-HUMAN ht09901.xl NCI CGAP Kid-13 Homo sapiens cDNA done IMAGE:3146256 3' similar to contains MER2913 MjR2grepeddve element; 3094 8245 13347 5,6 1.0OE-35 R67324.1 EST-HUMAN Y132c04M Soares placenta M2HP Horno sapiens cDNA done IMAGE:140934 3' 3094 8245 13348 5,6 1.OOE-35 R67324.1 EST HUMAN y132c04.si Soares placenta Nb2HP Homo saplens cDNA done IMAGE:140934 3' 3104 8254 13358 1.1 1.OOE-35 6006030 NT Homo saplens transcription elongation factor B (Sill), polypeptide 1-like (TCEB11 L) MRNA 4325 94M 14535 4.7 1.OOE-35 7656905 NT Muis musculus activin receptor interacting protein 1 (Aripl -pending), mRNA 4325 9448 14536 4.7 1.OOE-35 7656905 NT Mus musculus activin receptor interacting protein 1 1Adpi-pending), mRNA 2900 8051 13154 0.9 7.OOE-36 AW857579.1 EST-HUMAN CM1-CT0315-091299-063- d07 CT0315 Homo saplens cDNA 3080 8231 4.0 7.OOE-36 4557498 NT Homo saffiens C-temninal binding protein 2 (CTBP2) mRNA 1959 7076 12273 0.7 6.OOE-36 7706622 NT Homo sapiens ninjudn 2 (NINJ2), mRNA 2367 7469 2.2 6.0OE-36 A13035346.1 NT Homo sapiens TCL6 gene, exon 12 133 5329 10460 2,3 5.0OE-36 AJ271735.1 NT Homo sapiens Xq pseudoautosomal region; segment 112 2722 7806 13010 2.8 5.OOE-36 BE388436.1 EST - HUMAN 601285567F1 NII-LMW 44 Homo sapiens cDNA done IMAGE:3607289 S' 3561 8700 13796 2.0 5.OOE-36 AL163209.2 NT Homo saplens chromosome 21 segment HS21 C009 4678 9790 14890 1.4 5.OOE-36 5729729 NT Horno sapiens AM5-like 1 (AP]5L1), . mRNA 4678 9790 14891 1.4 5.OOE-36 5729729 NT Homo sapiens AP15-like 1 (API5L1), mRNA 1205 6337 11493 1.4 4.0012-36 BE010038.1 EST-HUMAN PM3-BN01 76-100400-001- 904 SNO1 76 Homo saplens cDNA 1424 6551 11728 0.6 4.OOE-36 P10266 SWISSPROT RETROVIRUS-RELATED POL POLYPROTEIN [CONTAINS: REVERSE TRANSCRIPTASE ENDONUCLEASE) 1626 6152 11921 0.6 4.0OE-36, BE382574.1 EST HUMAN 601298574F1 NIH MGC 19 Homo sapiens cDNA clone IMAGE:3628386 5, 7284 0.6 4.OOE-36 AW247772.1 EST---HUMAN 2820020.5prime N1H MGCJ Homo sapiens cDNA done IMAGE:2820020 S' Page 91 of 196 Table 4

2707 7792 12992 0.9 4.OOE-36 A1392661.1 EST HUMAN 1947b01.xl Soares NFL T GBC SI Homo sapiens cIDNA one IMAGE:2111881 X 2707 7792 12993 0.9 4.OOE-36 A1392661.1 EST-HUMAN!247b01.xl Soares_NFL. GBCLS1 Homo saplens cIDNA done IMAGE:2111881 3, 3310 8455 13568 3.2 4.OOE-36 BE389299.1 EST HUMAN 601282266F1 N11-1 MGC 44 Homo saplens cDNA done IMAGE:3604168 5' 3310 8455 13569 3.2 4.OOE-36 BE389299.1 EST-HUMAN 601282266F1 N1H MGC 44 Homo sapiens eDNA done IMAGE:3604168 5' 681 5836 10969 0.9 3.0OE-36 AF09981 0.1 NT Horno sapiens neure)dn 11 1- alpha gene, partial cels 2252 7358 12565 0.9 3.OOE-36 7662401 NT Homo sapiens KIAA0952 protein (K1AA0952), mRNA 3005 8157 13258 1.8 2.OOE-36 BE267054.1 EST HUMAN 601193463F1 N1H MGC 7 Horno saplens cIDNA done IMAGE:3537590 5' 3132 8281 13389 2.7 2.OOE-36 BE259267.1 EST HUMAN 601106343F1 N1H MGC 16 Homo sapiens cDNA done IMAGE:3342706 5' 4877 9984 15066 4.6 2.OOE-36 AW880376.1 EST HUMAN OVO-OT0030-240300-174- h04 OT0030 Homo sapiens cDNA 867 6015 11175 0.7 1.OOE-36 BE409310.1 EST-HUMAN 601300938F1 N11-1 MGC 21 Horno sapiens cDNA done IMAGE:363WO 5' 2098 7209 12416 0.9 1.OOE-36 BE146523.1 EST HUMAN RC1-HT0217-131199-021- h07 HT0217.Homo sapiens cDNA 2098 7209 12417 0.9 1.OOE-36 BE146523.1 EST HUMAN RC1 -HT0217-131199-021 - h07 HT0217 Homo sapiens CDNA 2155 7265 12474 1.3 1.OOE-36 AW205WA EST-HUMAN Ul-H-Bil-adt-e-09-0-Ul.sl NCI_QGAP_Sub3 Homo sapiens cDNA clone IMAGE:271M1 3, 5143 10238 15313 1.4 8.0OE-37 AB020684.1 NT, Homo sapiens mRNA for KIAA0877 protein, partial cds 1263 6392 0.9 7.OOE-37 AL042800.1 EST HUMAN DKFZp434E0422_rl 434 (synonym: htes3) Homo saplens cIDNA clone DKFZp434E0422 5' 2374 7476 12677 0.8 4.OOE-37 AA702794.1 EST HUMAN zi9ObO4.sl Soares-fetal- liver-spleenl INIFILSS1 Homo sapiens cDNA done IMAGE:448015 X 5160 10255 1.1 4.OOE-37 N62051.1 EST HUMAN EST52910 WATIVI1 Homo sapiens cDNA done 52g10 similar to human STS G04101 1970 7085 12287 1.0 3.OOE-37 AL048956.1 EST HUMAN DKRp4341-2418-rl 434 (synonym: htes3) Homo sapiens cIDNA done DKFZp434L2418 1970 7085 12288 1,0 3.OOE-37 A1-048956.1 EST-HUMAN DKFZp4341-2418-rl 434 (synonym: htes3) Horfl.o sapiens cDNA clone DKFZp4341-2418 2465 7563 0.6 3.OOE-37 AW961150.1 EST HUMAN EST373222 MAGE resequences, MAGF Homo.sapiens cIDNA 2936 8087 3.0 3.OOE-37 AW961150.1 EST HUMAN EST373222 MAGE resequences, MAGF Horno sapiens d3NA.

4072 9199 14285 1.0 3.OOE-37 8923313 NT Homo sapiens hypothetical protein FU20337 (FU20337), mRNA 1921 7038 12227 0.5 2.OOE-37 AL163247.2 NT Homo saplens chromosome 21 segment HS21 C047 3818 8955 14051 5.1 2.OOE-37 4503210 NT Homo saplens cytochrome P450, subfamily XWIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypepfide 1 (CY1P27A1 b) mRNA 1141 6277 11429 0.8 1.OOE-37 AA018293.1 EST-HUMAN ze40b05xl Soares retina N2b4HR Homo sapiens cIDNA done IMAGE:361425 5' 2041 7154 12360 1.3 1.OOE-37 AL163281.2 NT Homo sapiens chromosome 21 segment HS21 C081 3878 9014.14109 22.5 1.OOE-37 AF1 89011.1 NT lomo sa lens dbonudease Ill (RN3) mRNA, complete cds 2135 7245 12452 1.4 7.OOE-38 AW972825.1 EST HUMAN EST384920 MAGE resequences, MAGI- Homo saplens cDNA 710 5864 11000 1.2 5.OOE-38 AW971819.1 ESTHUMAN EST383908 MAGE resequences, MAGIL Homo sapiens cDNA 2404 7503 12710 0,7 5.OOE-38 AJ237740.1 NT Homo sapiens RIBIRgene (partial), exon 8 113 5314 10441 2.6 4.OOE-38 Z25466.1 NT B.taurus mitochonddal aspartate aminotransferase mRNA, complete CDS 113 5314 10442 2.6 4.OOE-38 Z25466.1 NT B.taurus mitochonddal aspartate aminotransferase mRNA, complete CIDS 1783 6903 1.4 4.OOE-38 AA582476.1 EST HUMAN nn54f12.sl NCI CGAP Kid6 Homo saplens cDNA done IMAGE:1087727 X 2053j.,71651 1.6 3.OOE-38 AF003530.1 NT Homo sapiens homeobox protein CDX4 (C1DX4) gene, oomplete cds and flanking repeat regions Page 92 of 196 Table 4

CMO 3638 8777 1.1 3.OOE-38 7549807 NT Homo sapiens HIRA Interacting protein 4 (dnaJ-Iike) (HIRIP4), mRNA 3781 8918 14012 1.7 3.OOE-38 P53538 SWISSPROT SSU72 PROTEIN 3781 8918 14013 1.7 3.OOE-38 P53538 SWISSPROT SSU72 PROTEIN 4513 9632 0.9 3.OOE-38 BE2793011.1 EST-HUMAN 601157633F1 NIH MGC 21 Homo sapiens cDNA done IMAGE:3504272 5' 44 5254 10368 1.4 2.OOE-38 AL163248.2 NT Homo sapiens chromosome 21 segment HS21C048 1358 6486 11652 0.9 2.OOE-38 5902097 NT Homo sapiens SMT3 (suppressor of mif two 3, yeast) homolog 2 (SMT3H2), mRNA 1627 6753 11922 0.6 2.OOE-38 AA437353.1 ESTHUMAN zw30d01.rl Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE:770785 5 similar to SW:MA12_RABIT P45701 MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2 MANNOSIDASE; 1627 6753 11923 0.6 2.OOE-38 AA437353.1 EST HUMAN zw30d01.rl Soares ovary tumor NbHOT Homo sapiens cIDNA done IMAGE:770785 5'sImilar to SW:1VIA12-RABIT P45701 MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2 MANNOSIDASE; 2580 7673 12875 0.7 2.OOE-38 W07055.1 EST-HUMAN za94a04xl Soares fetal lung_NbHL1 9W Homo sapiens cDNA clone IMAGE:300174 5, 1077 6215 0.8 1.0OE-38 AA401570.1 EST HUMAN zu62b02xl Soares-testis-NHT Horno sapiens cDNA done IMAGE:742539 Ysimilar to contains element MER19 repetitive element; 1954 7071 12268 0.9 1.OOE-38 4885288 NT Homo sapiens quanine nudeotide binding prote]n4ike 1 (GNLl), mRNA 1973 7088 12290 1.0 1.OOE-38 7661969 NT Homo sapiens KIAA0173 gene product (K1AA0173), mRNA 2016 7129 12329 0.8 1.OOE-38 7662129 NT Horno, sapiens KIAA0443 gene product (K1AA0443), mRNA 2445 7544 12753 0.6 1.OOE-38 AF270831.1 NT Homo sapiens cyclin K (CCNK) gene, exon 7 4063 9190 14275 1.4 1.OOE-38 A13037863.1 NT Homa sapiens mRNA for KIAA1442 protein, partial cds 4223 9347 14428 0.8 1.OOE-38 4505016 NT Homo sapiens low density lipoprotein receptor-related protein 6 (I-RP6) mRNA, and translated products 4229 9353 14434 1.3 1.0012-38 AL163203.2 NT Homo sapiens chromosome 21 segment HS21C003 4229 9353 14435 1.3 1.OOE-38 A1-163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 4493 9613 14705 1.2 1.OOE-38 8922543 NT Homo sapiens hypothetical protein FLJ10600 (FILA0600), mRNA 48 5258 10374 1.8 8.OOE-39 4502312 NT Homo sapiens ATPase, H+ transporting, lysosomal (vacuolar proton pump) 16W (ATP6C) mRNA 1372 6500 11666 0.6 8.OOE-39 4758229 NT Homo sapiens, estrogen receptor- binding fragment-associated gene 9 (EBAG9) mRNA 1788 6908 1.1 8.OOE-39 A1823404.1 EST HUMAN wh53f10.xl NCI - WAP_Kidl 1 Horno sapiens cDNA clone IMAGE:2384491 Ysimilar to TR:P87890 P87890 POL PROTEIN; 2047 7160 12364 2,0 7.OOE-39 AIL163227.2 NT Homo sapiens chromosome 21 segment HS21C027 991 6134 11289 1.3 5.OOE-39 AF003528.1 NT Homo sapiens X-linked anhidroltic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 2957 8108 13214 5.1 5.0012-39 A1750154.1 EST HUMAN at36b04.xl Barstead colon HPI-RI217 Homo sapiens cDNA done IMAGE:2374063 Ysimilar to TR:015408 Q15408 NEUTRAL PROTEASE LARGE SUBUNIT;contains LTR7.1:1 LTR7 repetitive element; 538 5701 10823 3.6 4.OOE-39 A13015610.1 NT Chlorocebus aethlops mRNA for ribosomal protein S4X, complete cds 3525 8666 13770 1.0 4.OOE-39 AL163210.2 NT Homo sapiens chromosome 21 segment HS21C010 1036211:::::3 d8 3.OOE-39 AA6349,49 4 we. ifc16 Regional genomic DNA specific cDNA library Homo sapiens cDNA done CR12-1 U Page 93 of 196 Table 4

41 5251 10363 3.8 3.0012-39 AA631949.1 EST-HUMAN WcliS Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR12-1 41 5251 10364 3.8 3.OOE-39 AA631949.1 EST HUMAN fmfol 6 Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR1 2-1 4902 10009 15089 2.7 3.OOE-39 AA313045.1 EST HUMAN EST183920 Pancreas tumor 1 Homo sapiens cDNA 5' end similar to similar to glucose transporter 879 6027 1.4 2.OOE-39 BE409203.1 EST-HUMAN 601301607F1 N11-1 M13C_21 Homo saplens cDNA done IMAGE:3636289 5' 894 6042 5,9 2.OOE-39 A1525119.1 EST - HUMAN promma-7.1301.r bvtumor Homo sapiens eDNA 5' 1015 6155 1.2 2.OOE-39 AF000573.1 NT Horno, sapiens homogentisate 1,2- dioxygenase gene. complete eds 1513 6640 3.7 2.0012-39 AW372318.1 EST HUMAN PMO-BT0340-211299-003-d02 BT0340 Homo sapiens cDNA 1930 7047 12239 10.0 2.OOE-39 AA720574.1 EST-HUMAN nw21g02.sl NCICi3APGWO Homo sapiens cDNA done IMAGE:1241138 3'similar to contains THR.t3 THR repetitive element; 2587 7680 12681 0.6 2.OOE-39 AL163248.2 NT Homo sapiens chromosome 21 segment HS21 C048 1497 6623 11793 0.6 1.0OE-39 A.1006345.1 NT Homo sapiens KVLOT1 gene 1497 6623 11794 0.6 1.OOE-39 AJ006345.1 NT Homo sapiens Wl-QT1 gene 1514 6641 11807 1.8 1.OOE39 7657020 NT Homo sapiens DKFZp434P211 protein (DKIFZP4341221 1), mRNA 2802 7954 13062 1.8 1.OOE-39 A1750362.1 EST HUMAN cn02cl O.y2 Normal Human Trabecular Bone Cells Homo saplens cDNA clone NHTSC cn02c10 random 2802 7954 13063 1.8 1.OOE-39 A1750362.1 EST HUMAN cn02cl O.y2 Normal Human Trabecular Bone Cells Homo sapiens cDNA done NI-ITBC cn02cl 0 random.

4561 9679 14771 5.5 1.0012-39 AW951995.1 EST-HUMAN EST364065 MAGE resequences, MAGB Homo saplens cDNA 4561 9679 14772 5.5 1.OOE-39 AW951995.1 EST HUMAN EST364065 MAGE resequences, MAGB Homo saplens cDNA 4604. 9718 14811 8.6 1.OOE-39 7657020 NT Homo sapiens DKFZp434P21111 protein (DKFZP434P21 1), mRNA 543 5706 10826 0.6 9.0OE-40 5803210 NT Homo sapiens UDP-plucose pyrophosehorylase 2 (UGP2), mRNA 1215 6345 11503 3,5 9.OOE-40 4755145 NT Homo sapiens AE-binding protein 1 (A1E13P1) mRNA 1215 6345 11504 3.5 9.0OE-40 4755145 NT Homo sapiens AE-binding protein 1 (AEBPl) mRNA 1433 6560 11737 1.8 9.OOE-40 4507512 NT Homo sapiens tissue Inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory) (TIMP3) mRNA 3892 10297 14118 166 9.OOE-40 AB033070.1 NT Homo sapiens mRNA for KIAA1244 protein, pardal ods 4250 9375 14455 0.8 9.OOE-40 4507848 NT Homo sapiens ubiquidn specific protease 13 (isopepddase T-3) (LISP1 3) mRNA 4398 9375 14455 1.1 9.OOE-40 4507848 NT Homo sapiens ubiquifin specific protease 13 (isopephdase T-3) (USP13) mRNA 3004 8156 13257 1.0 8.OOE-40 AA078165.1 EST-HUMAN 7H15A04 Chromosome 7 HeLa cDNA Library Homo sapiens cIDNA Clone 7H15A04 3847 8983 2.4 8.OOE-40 BE396541.1 EST HUMAN 601288958F1 NIH MGC 8 Homo sapiens cDNA done 14AGE:3619166 6 2696 7782 12981 1 6.OOE-40 AA361275.1 EST-HUMAN EST70527 T-cell lymphoma Homb sapiens cDNA Vend similar to similar to zinc finger protein family 2696 7782 12982 1.4 6.0OE-40 AA3611275.1 EST-HUMAN EST70527 T-cell lymphoma Homo sapiens cDNA Vend similar to similar to zinc finger protein family 2561 7655 1285g 0.7 5.OOE-40 AL163285.2 NT Homo sapiens chromosome 21 segment HS21 C085 6953 12139 1.4 4.OOE-40 A1686005.1 EST HUMAN -- tt91b01.xl NCLiCi3AP Pr28 Homo sapiens cDNA done IMAGE:2248873 Ysimilar to TR:073505 073505 P5L PROTEIN.; 1 Page 94 of 196 Table 4

1 L^ >0 2061 7173 1,0 4.OOE-40 AF003528.1 NT Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 4291 9415 14505 7.9 4.OOE-40 7662117 NT Homo sapiens KIAA0433 protein (K1AA0433), mRNA 1673 6797 11975 0,5 3.OOE-40 7705948 NT Homo sapiens rec (LOC51201), mRNA 2485 7582 12186 0,6 3.OOE-40 BE386406.1 EST HUMAN 601273576F1 N1H MGQ_20 Homo sapiens cDNA done IMAGE:3614635 S' 4040 9168 14253 1.0 3.OOE-40 A1925949.1 EST-HUMAN whl 2f07.xl NCI CGAP_Kidl 1 Horno sapiens cIDNA done IMAGE:2380549 3' 322 5505 4.3 2.OOE-40 A1223036.1 EST HUMAN g952h08.xl Soares tesde_NHT Homo sapiens cDNA clone IMAGE:1838847 3' 777 5929 0.9 2.0012-40 AW303868.1 EST HUMAN xr24e10.xl NCI - CGAP - UR Homo sapiens cDNA done IMAGE:2761098 Ysimilar to SW:1RS5 MOUSE P97461 40S RIBOSOMAL PROTEIN SS.

1633 6759 11929 0,7 2.OOE-40 AW964909.1 EST-HUMAN EST376877 MAGE resequences, MAGH Homo saplens cDNA 1633 6759 11930 0,7 2.OOE-40 AW964909.1 EST-HUMAN EST376877 MAGE resequences, MAGH Homo sapiens cDNA 1892 7009 12196 0.8 2.OOE-40 4506188 NT Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 7 (PSIVIA7) mRNA, and translated products 1892 7009 12197 0.8 2.OOE-40 4506188 NT Horno sapiens proteasome (prosome, rnacropain) subunit, alpha type, 7 (PSMA7) mRNA, and translated products 2026 7139 12339 1.1 2.0012-40 A1968562.1 EST-HUMAN wt90al 1.xl NCLWAP_GC6 Homo sapiens cDNA done IMAGE:2514716 Ysimilar to TR:Q91929 091929 ZINC FINGER PROTE1K..

2123 7233 12442 0,9 2.OOE-40 5453592 NT Homo sapiens adenylA cyclaseassociated protein 2 (CAP2) mRNA 2J33 7436 12644 0.9 2.OOE-40 AJ277892.1 NT Homo sapiens partial TTN gene for fitin 2658 7747 1.1 2.OOE-40 BE275932.1 EST-HUMAN 601121567F1 N1H MGC 20 Homo sapiens cDNA done IMAGE:3345784 5' 3087 8258- 13338 3.6 2.OOE-40 5453592 NT Horno sapiens adenylyl cyclase- associated protein 2 (CAP2) mIRNA 3913 6759 11929 6.4 2.0012-40 AW964909.1 EST HUMAN EST376877 MAGE resequences, MAGH Homo sapiens cDNA 3913 6759 11930 6.4 2.OOE-40 AW964909.1 EST-HUMAN EST376877 MAGE resequences, MAGH Homo saplens cIDNA 4807 9917 15010 1.5 2.OOE-40 AL-163280.2 NT Horno sapiens chromosome 21 segment HS21C080 4807 9917 15011 1.5 2.OOE-40 AL-163280.2 NT Horno sapiens chromosome 21 segment HS21 C080 865 6013 0.6 1.OOE-40 AA225989.1 EST-HUMAN no09a09.si NCI_PGAP PrIl Homo saplens cDNA clone IMAGE: 1007608 2653 7742 0,7 1.OOE-40 BE018348.1 EST-HUMAN 15b79a10.yl N11-1-1VIGQ_10 Homo sapiens cIDNA clone IMAGE:3048570 5'sImilar to TR:WZI158 Q9Z158 SYNTAXIN 17.; 3258 8405 1.8 1.OOE-40 4507142 NT Homo sapiens sorting n;x-Jn 3 (SNXJ) mIRNA 4505 9624 14716 6.3 1.OOE-40 4508012 NT Homo sapiens zJnc finger protein 200 (ZNF200) mRNA, and translated products 4892 9999 15082 0.9 1.0OE-40 7705778 NT Homo sapiens CGI-65 protein (LOC51103), mRNA 811 7900 11117 0.8 7.OOE-41 A1934364.1 EST HUMAN wp04h04.xl NCI CGAP Kidl 1 Homo sapiens cIDNA clone IMAGE:2463895 3, 811 7900 11118 0.8 7.OOE-41 A1934364.1 EST HUMAN wpO4h04.xl NCI CGAP Kidl 1 Homo sapiens cIDNA done IMAGE:2463895 3@ 279 5466 10596 0.6 6.OOE-41 AB037163.1 N Homo sapiens IDSCR5b mRNA, complete ods 2064 7176 12383 0.8 6.OOE-41 7657042 NT Homo sapiens Down syndrome candidate region, 1 (DSCR1), mIRNA 1761 6882 12064 1.3 5.OOE-41 T62628.1 EST HUMAN yc03e1O.si Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE:79626 3, 1957 7074 12271 1.8 5.OOE-41 AW843784.1 EST-HUMAN CM4-CN0044-21 01004 CN0044 Horno, saplens cDNA 4018 9147 1.0 5.OOE-41 4885636 NT Homo sapiens target of mybl (chicken) homol m 385 5554 0.7 4.0012-41 BE156318.1 EST_HUMAN OVO-HT0367-150200-114-909 HT0367 Horno sapiens cIDNA C), Page 95 of 196 Table 4 eo ow45e06.sl Soaresparathyroid tumo_NbHPA Homo sapiens cDNA clone IMAGE:1649794 3'sImilar to TR:000597 000597 CYTOCHROME C-LIKE POLYPEPTIDE,;contains LTR5.bl 1388 6515 11681 3.4 4.OOE-41 A1027117.1 EST-HUMAN LTR5 repetitive element; ow45c06.sl Soares_parathyroid_tumoLNbHPA Homo sapiens cDNA done IMAGE:1 649794 Y similar to TR.000597 000597 CYTOCHROME C-LIKE POLYPEPTIDE.;contains LTRS.bl 1388 6515 11682. 3.4 4.00E41 A1027117.1 EST-HUMAN LTR5 repetitive element; 1404 6531 11702 0.8 4.0OE-41 ABOO8681.1 NT Homo sapiens gene for actIvin receptor type 1113, complete ods tm96c04. x 1 NCI CGAP Bm25 Homo sapiens cDNA done IMAGE:2165958 3'similar to 1618 6744 11912 3,1 4.0012-41 A1500406.1 ESTHUMAN contains OFR.bOFR r7epefitive element; Homo sapiens 959 kb config between AMLI and CBR1 on chromosome 21q22; segment 113 2859 8010 13110 3.0 4.OOE-41 AJ229041.1 NT between AMLI and CBR1 on chromosome 21q22; segment 113 Homo sapiens 959 kb contig 2859 8010 13111 3.0 4.OOE-41 AJ229041.1 NT 1 4052 9179 14262 1.9 4.OOE-41 X92685.1 NT H.sapiens DNase 1 hypersensitive site (HSS-3) enhancer element 932 6079 11236 0.6 3.OOE-41 AB030176.1 NT Homo sapiens PAD-1-119 mRNA for pepfidylarginine deiminase type 11, complete cds Homo sapiens DNA, DLEC1 to ORCTI.4 gene region, section 112 (DLEC1, ORCTO, 4240 9365 14446 3.1 3.OOE-41 A8026898.1 NT ORCTL4 genes. complete cds) 5047 10147 0.9 3.OOE-41 AB037748.1 NT Homo sapiens mRNA for KIAA1 327 protein, partial cds 1782 6667 11837 2.6 2.OOE-41 U43701.1 NT Human dbosomal protein L23a mRNA, complete cds 1915 7032 12220 0.8 2.OOE-41 AA331940.1 ESTHUMAN EST35818 Embryo, 8 week 1 Homo sapiens cDNA C end 2172 7281 12494 1.0 2.OOE-41 AJ271366.1 NT Horno sapiens mRNA for putative adapt& protein (GRBIO gene) 2221 7328 12542 1.5 2,OOE-41 X89631.1 NT G. orilla DNA for ZNF80 gene homolog 2798 6667 11837 1.9 2.OOE-41 U43101.1 NT Human dbosomal protein L23a mRNA, complete cds 4521 9640 14731 1.1 2.OOE-41 AL163267.2 NT Homo sapiens chromosome 21 segment HS21C067 4521 9640 14732 1.1 2.OOE-41 AL163267.2 NT Homo saplens chromosome 21 segment HS21 C067 4465 9585 14681 4.6 1.0OE-41 6678468 NT Mus musculus tubulin alpha 6 (Tuba6), mRNA Homo sapiens homoobox protein COX4 (CDX4) gene, complete ods and flanking repeat 455 5621 10748 1.8 8 OOE-42 AF003530.1 NT regions Homo sapiens DNA, DLEC1 to ORCTI-4 gene region, section 112 (DLEC1, ORCTO, 2062 7174 12381 1.3 8.OOE-42 AB026898.1 NT ORCTL4 genes, corn 16te ods) 636 5795 10918 4.1 7.OOE-42 AF052051.1 NT Homo sapiens glutathlone transferase A4 gene, exon 1 636 5795 10919 4.1 7.OOE-42 AF052051.1 NT Homo sap ens glutathione transferase A4 gene, exon 1 916 6062 0.6 TOOE-42 AL163285.2 NT Homo sapiens chromosome 21 segment HS21 C085 1812 6931 12118 1.2 6.0OE-42 AF012872.1 NT Homo sapiens phosphaddylinositot 4-kinase 230 (p14K230) mRNA, complete ods mRNA, complete ods 1812 6931 12119 1.2 6.OOE-42 AF0112872.1 NT Homo sapiens phosphaddylinositot 4-kinase 230 (p14K230) xp29f0B.xl NCJ-CGAF_HNIO Homo sapiens cDNA done IMAGE:2741799 3'similar to 2244 7350 1.3 6.OOE-42 AW238656.1 EST-HUMAN contains Ll.tl Ll repetitive element; qu24h09.xl NCI_CGAP_8r12 Homo sapiens cDNA done IMAGE:1965761 similar to contains 6.OOE-42 A1284770.1 EST-HUMAN Alu repetitive element; 4936 10042 11.00 5.00E42 AJ271735.1 NT Homo saplens Xq_pgaudoautosomal region; segment 112 131 5327 1.9 V. nAC All EST HUMAN hv31 ell xl NCI CGAP Lu24 Homo sapiens cDNA done IMAGE:3175052 3' 5598 10119 Page 96 of 196 Table 4 o 478 5644 1.0 5.0012-42 5730038 NT Homo saplens SET domain and mariner transposase fusion gene (SETMAR) mIRNA 479 5645 1.3 5.0012-42 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 4884 9992 15073 1.1 5.OOE-42 A1541305.1 EST-HUMAN pecl.2-4.FOO.r ecnorm Homo saplens cDNA 5' 736 5890 11034 3.0 4.OOE-42 AF055066.11 NT Homo saplens WC class 1 region 736 5890 11035 3.0 4.OOE-42 AF055066.11 NT Homo sapiens WC class 1 region 1050 6189 11341 0.9 4.OOE-42 AF1 89011.1 NT Homo sapiens dbonuclease Ill (RN3) mRNA, complete cds 4100 9226 14311 1,6 4.OOE-42 X594117.11 NT H.sapiens PROS-27 mRNA 4156 9282 14362 4.5 4.OOE-42 4506496 NT Homo sapiens regulatory factor X, 4 (influences HLA class 11 expression) (R1FX4) mRNA 4480 9600 14694 10.3 4.OOE-42 4508008 NT Homo sapiens zinc finger protein 177 (ZNF177) mRNA 98 5306 1.0 3.OOE-42 AA486105.1 EST HUMAN abl4elO.sl Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE:840810 Ysimilar to contains THR.2 THR repetitive element; 2361 7463 1.4 2.OOE-42 AW898344.1 EST-HUMAN RC3-NNO070-270400-011-h10 NNO070 Homo sapiens cIDNA 2375 7477 12678 0.8 2.OOE-42 AW250059.1 EST-HUMAN 2819293.3prime N1H MGC 7 Homo sapiens cDNA done IMAGE:2819293 3' 717 5870 11010 1.1 1.OOE-42 X57147.1 NT Human endogenous retrovirus RHEA (ERV9) 1026 6166 11321 1.0 1.OOE-42 AW295809,1 EST-HUMAN Ul-H-BI 1-afh-e-M-0-Ul. sl NCI-CGAP-Sub3 Homo sapiens cIDNA clone IMAGE:2721871 3, 1085 6223 11374 1.1 11.0OE-42 A.1251818.1 NT Homo sapiens partial C9 gene for complement component C9, exon 1 1085 6223 11375 1.1 11.0OE-42 AJ25118118.1 NT Homo sapiens partial C9 gene for complement component C9, exon 1 1222 7910 11515 4,4 11.0OE-42 AF067166,1 NT Homo sapiens NADH-ubiquinone oxidoreductase AGGG subunit precursor homolog mRNA, nuclear gene encoding mitochondrial protein, complete cds 1222 7910 11516 4.4 1.0OE-42 AF067166.1 NT Homo sapiens NADH.ubiquinone oxldoreductase AGGG subuffit precursor homolog mRNA, nuclear gene encoding mitochonddal protein, complete cds 1986 7101 12304 0.9 11.0OE-42 AF1 10296.1 NT Homo sapiens PIDINIP1 gene, exon 17 2497 7594 12796 0.7 11.0OE-42 5174458 NT Homo saplens major histocompatibility complex, class li, DIVII alpha (HLA-DMA) mRNA 2935 8086 13194 8.9 11.0OE-42 4505524 NT Homo sapiens origin recognition complex, subunit 5 (yeast homolog)-Uke (ORCSL) mRNA, and translated products 3647 8786 13879 2,2 11.0OE-42 7662027 NT Homo sapiens KIAA0255 gene product (K1AA0255), mRNA 3849 8985 14085 1.0 1.OOE-42 AL163267.2 NT Homo sapiens chromosome 21 segment HS21 C067 4153 9279 14359 1.7 11.0OE-42 AL163280.2 NT Homo sapiens chromosome 21 segment HS21 C080 4489 W59 14702 0.9 11.0OE-42 AW813617.1 EST-HUMAN RC3-ST0197-161099-012- a03 ST0197 Homo sapiens cDNA 4640 9754 14848 2,9 11.0OE-42 5803122 NT Homo sapiens proteasome Inhibitor (P131), mRNA 4640 9754 14849 2.9 1.OOE-42 5803122 NT Homo sapiens proteasome Inhibitor (P131), mRNA 4669 9781 14880 5.4 11.0OE-42 4506758 NT Homo saplens ryanodine receptor 3 (RYR3) mRNA 4769 9880 14974 1.2 1.OOE-42 AB033114.1 NT Homo sapiens mRNA for KIAA1288 protein, partial cds 5048 10148 15225 1.0 11.0OE-42 4501912 NT Homo sapiens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 5048 10148 15226 1.0 11.0OE-42 4501912 NT Homo sapiens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 685 5840 10973 1.5 8.OOE-43 8923276 NT Homo saplens hypothetical protein FLJ20297 (FLJ20297), mRNA 685 5840 10974 1,5 8.OOE-43 8923276 NT Homo saplens hypothetical protein FLJ20297 (FU20297), mIRNA 685 5840 10975 1.5 8.0012-43 8923276 NT Homo sapiens hypothetical protein FLJ20297 (FU20297), mIRNA 3 8728 13824 6.1 7.OOE-43 AW246442.1 ESTHUMAN 28222511.5prime N1H MGC 7 Homo sapiens cDNA clone IMAGE 2822251 5' Page 97 of 196 Table 4 rZ>0 >0 1320 6449 3,7 6.OOE-43 AA491890.1 EST HUMAN ne72d06.sli NCI_WAP_Ewl Horno sapiens cONA done IMAGE:909803 similar to gb105095 60S RIBOSOMAL PROTEIN L30 (HUMAN); 137 5333 0.7 5.OOE-43 AL163213.2 NT Homo sapiens chromosome 21 segment S21C013 494 5659 10781 1.0 5.OOE-43 AA382780.1 EST-HUMAN EST96033 Testis 1 Homo sapiens cDNA 5' end.

957 7865 11260 1.7 4.OOE-43 AF003528.11 NT Homo sapiens X-linked anhidroldc ectodermal dysplasla protein gene (EDA), exon 2 and flanking repeat regions 714 5868 11006 3.3 3.OOE-43 AA083568.11 EST HUMAN zn09h09.sli Stratagene hNT neuron (#937233) Homo sapiens cIDNA. done IMAGE:546977 3' similar to TR:W 136285 G1 136285 UDP-GAI-NAC:POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE. [1]; 1195 6327 1.0 3.OOE-43 AF2233911.1 NT Homosapiens calcium channel alphalE subunit (CACNA1E) gene, exons 7-49, and partial cds, alternatively spliced 1669 6793 11969 0.5 3.OOE-43 X97869.11 NT H.saplens gene encoding La autoandgen 3524 8665 13769 1.1 3.OOE-43 S69002.11 NT AMI-II-EVI-11=AMLI-EVI-11 fusion protein (rearranged translocation) [human, leukernic cell line SKH11, mRNA Mutant, 5938 nt) 4193 9319 14404 1,0 3.OOE-43 AA548154.1 EST-HUMAN nk55d06.sli NCI CGAP Pr7 Homo sapiens cIDNA done IMAGE:1017419 177 5370 1.5 2.OOE-43 AI 190764,1 EST-HUMAN qd61c09.xl Soares-tesds-NHT Horno sapiens cDNA done IMAGE:1733968 Ysimilar to contains PTR7.t3 PTR7 PTR7 repetitive element; 1 1630 6756 11926 1.1 11.0OE-43 AF154836.1 NT Homo saplens Ras-like GTPbinding protein (RAB27A) gene,.exons 1 b and 2 1630 6756 11927 1.1 1.00E43 AF154836.1 NT Homo sapiens Ras-like GTP- binding protein (RA1327A) gene, exons I b and 2 1678 6802 11982 1.2 1.0OE-43 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 872 6020 11180 2.0 8.00E44 A1222985.11 EST-HUMAN qh23901. xl Soares NFL T GBC S1 Homo saplens cDNA clone IMAGE:1845552 3' 872 6020 11181 2.0 8.0OE-44 A1222985.11 EST HUMAN qh23g011,x11 Soares NFL T GBC S1 Homo sapiens cIDNA clone IMAGE: 1845552 3' 643 5802 0,9 7.OOE-44 R06035.11 EST-HUMAN ye89c01.rl Soares fetal liver spleen 1INIFILS Ho. mo sapiens cIDNA clone IMAGE: 124920 5' 2187 7296 12510 1.2 7.OOE-44 5031886 NT Homo sapi ens LIM domain- containing preferred translocation partner in lipoma (LPP) mRNA 2937 8088 13195 2.2 7.OOE-44 AF048729.1 NT Homo saplens minisatellite ms32 repeat region 2937 8088 13196 2.2 7.OOE-44 AF048729.1 NT Homo sapiens minisatellite ms32 repeat region 3786 8923 14017 2,3 7.00E44 A1.163284.2 NT Homo sapiens chromosome 21 segment HS21C084 4148 9274 14353 1.2 7.OOE-44 AF231 919.1 NT Homo sapiens chromosome 21 unknown mRNA 4148 9274 14354 1.2 7.OOE-44 AF231 919.1 NT Homo saplens chromosome 21 unknown mRNA 300 5486 0.9 5.OOE-44 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZ71-11 gene 329 5510 0.7 5.00E44 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTIFIL11 gene 3368 8511 13618 2.9 4.OOE-44 AL163303.2 NT Homo sapiens chromosome 21 segment HS21 Cl 03 1746 6868 1,1 3.00E44 6912477 NT Homo sapiens karyopherin alpha 6 (Importin alpha 7) (KPNA6), mIRNA 3059 8210 13309 5,1 3.OOE-44 AA169851.1 EST HUMAN zpl8b05.rl Stratagene fetal retina 937202 Homo sapiens cIDNA clone IMAGE:609777 C 3396 8538 13644 1.5 3.OOE-44 AA683268.11 EST.HUMAN ae92b04.sl Stratagene schizo brain $11 Homo sapiens cIDNA done IMAGE:1020367 3, 3396 8538 13645 1.5 3.OOE-44 AA683268.11 ESTHUMAN ae92b04.sl Stratagene schizo brain S1 1 Homo sapiens cIDNA done IMAGE:1020367 3' 1.4 2.OOE-44 W28189.1 EST HUMAN 43c5 Human retina cIDNA randomly primed sublibrary Horno saplens cIDNA j 11325 0.9 2.0OE-44 4826685 NT Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/H1s) box polye!pdde 1 (DDX1) mIRNA 12PL-,6173 Page 98 of 196 Table 4

IZi' (Z>10 1033 6173 11326 0.9 2. OOE-44 4826685 NT Homo sapiens DEAD/H (Asp-Glu-Ala- Asp/His) box polypIptide 1 (DDX1) mRNA 1189 6321 11477 1.6 2.OOE-44 5803200 NT Homo sapiens transmembrane trafficking protein (TMP21), mRNA 1189 6321 11478 1.6 2.0012-44 5803200 NT Homo sapiens transmembrane trafficking protein (TMP21), mRNA 1290 6419 11584 1.0 2.OOE-44 AF133588.1 NT Homo sapiens RAB36 (RAB36) mRNA, complete cds 1346 6474 11640 0.5 2.OOE-44 BE465325.1 EST-HUMAN hw14g06.xl NCI_PGAP_Lu24 Homo saplens cDNA done IMAGE:3182938 3'similar to SW:0XY13-HUMAN P22059 OXYSTEROL-BINDING PROTEIN.; 2105 7216 12426 0.7 2.OOE-44 AF070651.1 NT Homo sapiens tissue-type bone marrow dric finger protein 4 MRNA, complete cds 2529 7625 12826 1.1 2.0012-44 D25303.1 NT Human mRNA for Integrin alpha subunit, complete cds 2567 7660 1.2 2.OOE-44 5901933 NT Homo sapiens adaptor-related protein complex 4, sigma 1 subunit (CLAPS4), RNA 3425 8567 13679 1.4 2.OOE-44 D87675.1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 4468 9588 14684 1.8 2.OOE-44 AW864379.1 EST-HUMAN PM4-SW01 6-120500-003- a04 SNO01 6 Homo sapiens cIDNA 46 5256 10371 1.2 1.0OE-44 7657334 NT Homo saplens Misshapen/NK-related kinase (MINK), mRNA 46 5256 10372 1.2 1.00E44 7657334 NT Homo sapiens Misshap6n/NIK-related. kinase (MINK), mRNA 566 5727 10844 0.6 1.OOE-44 AW853132.1 EST-HUMAN RCI-CTO249-030300-026- h12 CTO249 Homo sapiens cIDNA 1179 6312 0.5 1.OOE-44 AW994803.1 EST-HUMAN RC1-BN0039-110300-012-b01 BN0039 Homo sapiens cIDNA 1555 6682 2.0 1.0OE-44 AL163503.2 NT Homo sapiens chromosome 21 segment HS21 10 2177 7286 12497 1.3 1.OOE-44 AA434554.1 ESTHUMAN zw53d02.rl Soares_total- fetus-Nb2HF8-gw Homo sapiens cIDNA done IMAGE:773763 & similar to contains THR.1:3 THR repetitive element; 2177 7286 12498 1.3 1.OOE-44 AA434554.1 EST-HUMAN zw53d02xl Soares - total fetus - Nb21-IF0_9w Homo sapiens cDNA done IMAGE:773763 5, similar to contains THR.t3 THR repetitive element; 2237 7938 12556 1.1 1.OOE-44 AA398099.1 ESTHUMAN zt88gl 1xl Soares tests NHT Homo sapiens cDNA done IMAGE:729476 5' 2732 7816 13021 1.4 1.00E44 AF196779.1 NT Homo sapiens transcription factor IGHM enhancer & JM1 1 protein, JM4 protein, JM5 protein, T54 protein, JIM10 protein. A4 differentlation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, complete cds; and L-type calcium channel a> 366.4 8803 5.1 1.0OE-44 AA455869.1 EST-HUMAN aa01 cOg.sl Soares_Nhi-IMPu S1 Homo sapiens cDNA done IMAGE:811984 3' 5061 10159 15234 0.8 1.OOE-44 AJ130755.1 NT Homo sapiens alpha satellite DNA, M1 monomer type 5061 10159 15235 0.8 1.OOE-44 AJ130755.1 NT Homo sapiens alpha sa Ilite DNA, M1 monomer type 4476 9596 14691 1.7 9.OOE-45 8922391 NT Homo saplens hypothefical_protein FU10379 (FLJ10379), mRNA 4476 9596 14692 1.7 9.OOE-45 8922391 NT Homo sapiens h)Tothefical protein FLJ10379 (FLA0379), mRNA 2477 7575 12777 1.4 8.OOE-45 5174718 NT Homo sapiens TRK-fused gene (NOTE: non-standard symbol and name) (TFG) mRNA 5015 10117 15188 7.5 8.OOE-45 5174718 NT Homo sapiens TRK-fused gene (NOTE: non-standard symbol and name) (TFG) mRNA 3896 9031 5.3 6.OOE-45 AW157570.1 EST-HUMAN au83h07.xl Schnolder fetal brain 00004 Horno sapiens cIDNA done IMAGE:2782909 Y similar to SCR1 3A HUMAN P40429 60S RIBOSOMAL PROTEIN L1 3A; 874 6022 1.1 5.OOE-45 AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 3173 8322 13436 2.0 5.OOE-45 A1523766.1 EST HUMAN t994f07.xl NCI - CGAP - CLI-11 Homo sapiens cDNA done IMAGE:2116453 Ysimilar to SW:1DAXI. MOUSE P09084 PAIRED BOX PROTEIN PAX-1, 1127 6263 11415 3.1 4.OOE-45 X95826.1 NT H.saple 7ART4 wene 2246 7352 12559 0.7 4.OOE-45 BE265622.1 EST HUMAN 6011 OF1 N11-1 MGC 7 Homo sapiens cDNA done IMAGE:3538425 5' 11 6322 0.9 3.OOE-45 AW500307.1 EST HUMAN Ul-HF-BNO-akg-d-02-0-Ul.rl N111-1VIGC50 Homo sapiens cDNA done IMAGE:3077019 V Page 99 of 196 Table 4

3997 8434 1.2 3.OOE-45 T71480.1 EST HUMAN yd35f07xl Soares fetal liver spleen INFLS Homosaplens.ciDNA clone IMAGE:1102455' 4682 9794 14896 1.1.3.OOE-45 BE297384.1 EST-HUMAN 601178009F1 N1H MMJ7 Homo sapiens cDNA done IMAGE:3533091 V 4682 9794 14897 1.1 3.00E45 BE297384.1 EST-HUMAN 601178009F1 N11-1 MGC 17 Homo saplens cIDNA done IMAGE:3533091 5' 2454 7553 0.8 2.00E45 AL163218.2 NT Homo sapiens chromosome 21 segment HS21 C01 8 2996 814W 13248 0.9 2.OOE-45 AJ243213.1 NT Homo, sapiens partial 5-HT4 receptor gene, exons 2 to 5 3509 8650' 13760 0.8 2.OOE-45 BE004686.1 EST-HUMAN CM1 -BN01 1 1-240300- 161-b04 BN01 11 Homo sapiens cIDNA 118 5571 0.9 1 OOE-45 BE389855.1 EST HUMAN 601284360F1 N11-1 MGC 44 Homo, sapiens cDNA done IMAGE:3606183 S' 403 5571 0.8 1.0012-45 BE389855.1 EST-HUMAN 601284360F1 N11-1 MGC 44 Homo sapiens cDNA clone IMAGE:3606183 S' 464 5629 10759 0.5 1.00E45 4506412 NT Homo saplens RAP1A, member of RAS oncogene family (RAP1A), mRNA 1157 6292 11447 0.6 1.0012-45 7657290 NT Homo sapsens Langerhans cell specific c-type lectin (LANGERIN), mRNA 3065 8216 13316 6.8 1.00E45 U321169.1 NT Human pro-a2 chain of collagen type Xl (COL1 1A2) gene, complete cds 3266 8413 13519 8.2 1.0012-45 Y16641.1 NT Rattus norvegicus mRNA for hnRNP protein, partial 3447 8589 13103 1.0 1.00E45 8659558 NT Homo sapiens chromosome 21 open reading frame 1 (C21orf4), mRNA 4378 9500 14587 4.1 1.00E45 BE396633.1 EST-HUMAN 601289116F1 N1H WC. i Horno, saplens cIDNA done IMAGE:3619803 5' 2392 7492 12697 3.2 8.OOE-46 A1433261.1 EST-HUMAN E32f08.xl NCI CGAP - Gas4 Homo sapiens cIDNA done IMAGE:2132199 Ysimilar to gb:J00314 mj2 TUBULIN BETA-1 CHAIN (HUMAN); 2392 19 12698 3.2 8.OOE-46 A1433261.1 EST-HUMAN U32f08.xl NCI - CGAP_Gas4 Homo sapiens cDNA done IMAGE:2132199 X similar to 7492 gb:J00314 rna2 TUBULIN BETA-1 CHAIN (HUMAN); 4478 98 6.5 7.0012-46 BE386165.1 EST-HUMAN 601277292F1 N11-1 MGQ_20 Homo saplens cIDNA clone IMAGE:3618119 5, 9598 4701 9813 1.0 7.OOE-46 BE064386.1 EST-HUMAN RC4-13T0310-110300-015410 BT0310 Homo sapiens cDNA 2726 1 13015 2.0 6.OOE-46 A1884381.1 EST-HUMAN wrn31f08.xl NCl-WAP-UW Homo saplens cIDNA clone IMAGE:2437575 Ysimilar to 7810 contains MER19A2 MER19 repetitive element; 2726 7810 13016 2.0 6.OOE-46 A1884381.1 EST-HUMAN wm31f08.xl NCLWAPUR Homo sapiens cIDNA done IMAGE:2437575 Ysimilar to contains MER19A2 MER19 repetitive element; 197 5391 2.2 5.OOE-46 AL1 63210.2 NT Homo sapiens chromosome 21 segment HS21C010 625 5784 0.5 4.OOE-46 AA601143.1 EST HUMAN no54e09.sl NCLWAP-SS1 Homo saplens cIDNA done IMAGE:1 104520 3'similar to gb:X53741 mal FiBULINA, ISOFORM A PRECURSOR (HUMAN); 1676 6800 11978 1.3 4.00E46 AW770544.1 EST HUMAN W86c03.xl NCI CGAP Lu24 Homo sapiens cDNA done IMAGE:3008836 Ysimilar to gb:X14008-mJ LYSO-ZYME C PRECURSOR (HUMAN);contains element MER37 repetitive element; 1676 6800 11979 1.3 4.OOE-46 AW770544.1 EST-HUMAN W86c03.xl NCI - CGAP - Lu24 Homosapiens cDNA done IMAGE:3008836 Ysimilar to gb:X14008-mal LYSOZYME C PRECURSOR (HUMAN);contains element MER37 repetitive element; 2710 7795 12996 1,3 4.OOE-46 M18048.1 NT Human endogenous retrovirus RTW- 1-12 4320 9443 14531 1.1 4.00E46 A13014522.1 NT Homo sapiens mRNA for KIAA0622 protein, partial cds 4320 9W_ 14532 lel 4.00E46 A13014522.1 NT Homo sapiens mRNA for KIAA0622 protein, partial ods 4294 9418 14508 0.8 3.00E46 4506376 NT Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), mRNA 3.OOE-46 Z73660.1 NT H.sapiens 19 lambda light chain variable region gene (7c.1 1.2) germline;]g-Light-Lambda; Vi-ambda a Page 100 of 196 Table 4

4666 9778 14876 1,1 3.OOE-46 Z73660.1 NT H.sapiens lg lambda light chain variable region gene (76.11.2) germline; lg-Light-Lambda; VLambda 819 5969 11126 2.0 2.OOE-46 AA468646.1 EST-HUMAN neO6a09.sl NCI CGAP_po3 Homo saplens cDNA done IMAGE:880408 Ysimilar to contains THR.b:THR repetitive element; 1542 6669 1.3 2.OOE-46 AA678246.1 EST HUMAN 7J27al I.sl Soare5_fetaLliveLspieen_1NFLS-S1 Homo sapiens cDNA clone IMAGE:431996 3' 1623 6749 11917 0,9 2.00E46 U78027.1 NT Homo sapiens Bruton's tyrosine kinase (M), alpha-D-galactosidase A (GLA), L44-like dbosomal protein (L441---)and FTP3 (FTP3) genes, complete cds 4199 9329 14412 1.0 2.OOE-46 AF016191.1 NT Rattus norvegicus potassium channel (er93) mRNA, complete cds 4899 10006 15087 1.2 2.OOE-46 AA399286.1 EST HUMAN zt59c02.rl Soarestestis-NHT Homo saplens cDNA clone IMAGE:726650 V similar to SW:RSP1 MOUSE 001730RSP-1 PROTEIN,; 1213 6343 11502 1.8 1.0OE-46 4502694 NT Homo sapiens cell division cycle 10 (homologous to WC10 of S. cerevislae) (CIDC10) mRNA 2236 7343 12555 1.7 1.OOE-46 AW978516.1 EST HUMAN EST390625 MAGE resequences, MAGP Homo sapiens cDNA 2351 7453 12660 0.9 1.00E46 H97330.1 EST HUMAN EST48b095 WATM1 Homo sapiens cDNA done 48b095 3211 8359 13468 2,8 1.OOE-46 AA631912.1 EST - HUMAN rip78b02.sl NCLWAP " Pr2 Homo sapiens cDNA done IMAGE: 1132395 similar to gb:X76717 H.sapiens Nfr-11 mRNA. (HUMAN); 4772 9882 2.6 1.00E46 AB023197.1 NT Homo sapiens mRNA for KIAA0980 protein, partial cds 749 5901 1.5 9.OOE-47 A.1271735.1 NT Homo sapiens Xq pseudoautosomal region, segment 112 4848 9955 15044 2.6 9.OOE-47 AW770928.1 EST-HUMAN h193904.xl NCI-CGAP- LU24 Homo saplens cIDNA clone IMAGE:3009534 Ysimilar to TR:075703 075703 HYPOTHETICAL 12.4 KI) PROTEIN.; 1766 6887 12071 5.1 8.00E47 Y18536.1 NT Homo sapiens HLA-C gene, exon 5, individual 19323 1766 6887 12072 5.1 8.OOE-47 Y18536.1 NT Homo sapiens HLA-C gene, exon 5, Individual 19323 2684 7771 12971 0.6 8.OOE-47 5453955 NT Homo sapiens protein phosphatase 2, regulatory subunit B (856), epsilon isoform (PPP2R5E) mRNA 2989 8141 13244 1.7 8.OOE-47 AJ229043.1 NT Homo saplens 959 kb config between AM0 and CBR1 on chromosome 21q22, segment 313 693 5848 10983 2.0 6.OOE-47 7705390 NT Homo sapiens H91 620p protein (H91 620p), mRNA 2501 7598 12798 1.1 6.OOE-47 AL1 63246.2 NT Homo sapiens chromosome 21 segment HS21C046 1379 6506 11672 1.2 4.0012-47 4557556 NT Homo sapiens E1A binding protein p300 (12P300) mRNA 171 7882 0.6 3.OOE-47 BE385662.11 ESTHUMAN 601278193F1 N1H MGC 20 Homo, sapiens cDNA done IMAGE:3618934 5 801 5952 11103 1.8 3.OOE-47 N57483.1 EST-HUMAN yy54b04.sl Soaresmuidplesderosis-2NbHMSP Horno sapiens cDNA done IMAGE:277327 3' 9jo 6077 11236 3.0 3.OOE-47 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 1984 7099 12302 0.5 3.0OE-47 A13007899.1 NT Homo sapiens KIAA0439 mRNA, partial cds 3235 8383 13489 5.1 3.OOE-47 AA304095.1 EST-HUMAN EST1 6895 Aorta endothelial cells, TINIF alpha-treated Homo sapiens eDNA 5' end similar to similar to metallopanstimulln 1 3885 9021 5.0 3.OOE-47 U931811.1 NT Homo sapiens nuclear duall- specificity phosphatase (SBF1) mRNA, pardal cds 4265 9390 14473 1,0 3.OOE-47 M12959.1 NT Human T-cell receptor active alpha-chain mRNA from JM cell line, complete cds idHomo 142 5338 10468 1.5 2.OOE-47 4505318 NT Homo saplens myosin phosphatase, target subunit 2 (MYPT2), mRNA 098 11254 0.7 2.OOE-47 AIL163209.2 NT saplens chromosome 21 segment HS21C009 ----7_T Page 101 of 196 Table 4

952 6098 11255 0.7 2.OOE-47 AL1 63209.2 NT Homo sapiens chromosome 21 segment HS21 C009 1548 6675 1.2 2.OOE-47 A1969279.1 EST-HUMAN wq96b02.xl NCI CGAP GC6 Homo saplens cDNA clone IMAGE:2479851 X 1575 6701 11870 1.2 2.OOE-47 7662109 NT Homo sapiens KIAA0426 gene product (K1AA0426), mRNA 1654 6779 11952 1.6 2.OOE-47 AA524514.1 EST-HUMAN nq43h12.s11 NCI CGAP Co3 Homo, sapiens cDNA done IMAGE:937607 3' 2185 7294 12506 0.8 2.OOE-47 AF060568.11 NT Homo sapiens promyelocytic leukemia zJnc finger protein (PLZF) gene, complete cds 2620 7710 12910 0.6 2.OOE-47 AA077092.1 EST HUMAN 7B07G09 Chromosome 7 Fetal B 1 rain cDNA Library Homo saplens cDNA clone 7B07G09 4251 9376 14456 1,7 2.OOE-47 4504866 NT Homo sapiens ring finger protein (C21HC4 type) 8 (RNF8), mRNA 4287 9412 14499 1.8 2.OOE-47 AA569592.1 EST HUMAN nf23907.sl NCI CGAP PrI Homo sapiens cDNA done IMAGE:914652 4287 9412 14500 1.8 2.OOE-47 AA569592.1 EST HUMAN nf23g07.sl NCI CGAP PrI Homo sapiens cDNA clone IMAGE:914652 4406 9526 14616 2,1 2.OOE-47 5174648 NT Homo saplens ReWRex activation domain binding protein-related (RAB-R) mRNA 4707 9819 14916 1.1 2.OOE-47 AW965166.1 EST-HUMAN EST377239 MAGE resequences, MAGI Homo sapiens cDNA 1383 6510 11674 1.6 1.OOE-47 A1333429.1 EST HUMAN qp99h03.xl SoaresfetaL]ung_NbHL19W Homo sapiens cDNA clone IMAGE:1931189 3' 3749 8887 13975 0.8 1.0OE-47 BE280477.1 EST HUMAN 601155321 F1 N1H MGC 21 Horno, sapiens cDNA done, IMAGE:3138893 5, 3749 8887 13976 0.8 1.OOE-47 BE280477.1 EST HUMAN 601155321 F1 NIH MGCj21 Homa, sapiens cDNA done IMAGE:3138893 5' 5008 10110 15180 2.6 1.OOE-47 AW813906.1 ES-_HUMAN RC3-ST01 97-130400-017h02 ST01 97 Homo saplens cIDNA 1595 6721 11890 0.9 9.OOE-48 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNA E) gene, exons 7-49, and partial cds, alternatively spliced 1236 6360 0.5 8.OOE-48 4501900 NT Homo sapiens aminoacylase 1 (ACY1), mRNA 1231 6360 1.5 8.OOE-48 4501900 NT Homo sapi ns aminoacylase 1 (ACY1), mRNA 3096 8247 13350 3.6 8.OOE-48 AW768477.1 EST-HUMAN hk61b03.xl NCI_CGAP_Lyml2 Homo sapiens cDNA done IMAGE:3001133 3' similar to gb:X64707 BREAST BASIC CONSERVED PROTEIN 1 (HUMAN); 3096 8247 13351 3.6 8.OOE-48 AW768477.1 EST-HUMAN hk61b03.xl NCI_. PGAP_Lym12 Homo saplens cDNA clone IMAGE:3001133 X similar to gb:X64707 BREAST BASIC CONSERVED PROTEIN 1 (HUMAN); 482 5648 1.4 7.OOE-48 A13033035.1 NT Homo sapiens mRNA for KIAA1209 protein, partial cds 483 5648 4.6 7.OOE-48 A13033035.1 NT Homo sapiens mRNA for KIAA1209 protein, partial cds 1482 6609 11782 1.1 7.OOE-48 6912719 NT Homo sapiens tousled-like kinase 1 (TW), mRNA 1620 6746 11914 1.3 7.OOE-48 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 1551 6678 11846 0.8 6.OOE-48 AF163441,1 NT Homo sapiens golgin 67 mRNA, complete cds 3269 10295 13521 1.4 5.OOE-48 4826891 NT Homo sapiens phosphodiesterase 1A, calmodulin-dependent (PDEIA) mRNA 1498 6624 1.3 4.OOE-48 AA1 57453.1 EST HUMAN zo55c04.sl Stratagene pancreas (#937208) Homo sapiens cDNA done IMAGE:590790 3' similar to contains Ll.t3 L1 repetitive element; 1933 7050 12242 6.9 3.OOE-48 4885170 NT Homo saplens chromosome X open reading frame 6 (CXORF6) mRNA 1933. 7050 12243 6.9 3.OOE-48 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 3579 8718 13816 0.9 3.OOE-48 AW664531.1 EST-HUMAN W14b12.xl NCI - CGAP- GUI Homo sapiens cDNA clone IMAGE:2972255 3'similar to SW:DCRB HUMAN P56555 DOWN SYNDROME CRITICAL REGION PROTEIN B.; 39 5249 10359 1.2 2.OOE-48 AA631940.1 EST-HUMAN fmfc7 Regional genomic DNA specific cDNA library Homo sapiens cDNA done CR17-26 1752 6874 12054 0.9 2.0012-48 AA348396.1 EST HUMAN ESTS4841 Hippocampus 11 Homo saplens cDNA 5' end 6874 12055 0.9 2.OOE-48 AA348396.1 ESI-HUMAN ESTS4841 Hippocampus 11 Homo sapiens cDNA 5' end 1 Page 102 of 196 Table 4 -A 4431 9552 14646 1.4 2.OOE-48 BE246065.1 EST HUMAN TCBAP1D3842 Pediatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project=TCBA Homo sapiens cIDNA clone TCBAP3842 5259 10376 0.9 1.0OE-48 7706534 NT 1 Homo sapiens cisplatin resistance-associated overexpressed protein (LOC51747), mRNA 855 6004 11164 2.4 1.OOE-48 4502166 NT Homo sapiens amylold beta (A4) precursor protein (protease ne)dn-l I, Akheirner disease) (APP), mRNA 1274 6404 11564 1.1 1.0012-48 5032032 NT Homo sapiens RNA binding modf protein 6 (R13M6) mRNA 1876 6994 12180 16.0 1.00IE-48 AL1 63302.2 NT Homo sapiens chromosome 21 segment HS21 Cl 02 3443 8585 13699 1.2 1.OOE-48 AL163246.2 NT Homo saplens chromosome 21 segment HS21 C046 5082 10180 15260 1.1 1.OOE-48 M10976.1 NT Human endogenous retroviral DNA (4-11), complete retroviral segment 134 5557 10689 1.5 7.OOE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSIVIC4) mRNA 134 5557 10690 1.5 7.OOE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSIVIC4) mRNA 388 5557 10689 0,6 7.OOE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSIVIC4) mRNA 388 5557 10690 0.6 7.OOE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSIVIC4) mRNA 389 5557 10689 0.7 7.00IE-49 572 9990 NT Homo saplens proteasome (prosome, macropain) 26S subunit, ATPase. 4 (PSIVIC4) mRNA 389 5557 10690 0.7 7.OOE-49 5729990 NT Homo sapiens proteasome (prosome, macropaJn) 26S subunit, ATPase, 4 (PSIVIC4) mRNA 1202 6334 11490 1.4 7,OOE-49 AL163284.2 NT Homo saplens chromosome 21 segment HS21 C084 2129 7239 12447 0.9 7.OOE-49 T75294.1 EST HUMAN yc89b07xl Soares Infant brain 1 NIB Homo sapiens cIDNA done IMAGE:23233 5' similar to SPACUkPENCH P36333 ACETYL-COENZYME A SYNTHETASE; 4550 9669 14759 1.0 7.OOE-49 060811 SWISSPROT HYPOTHETICAL PROTEIN DJ845024.3 5384 10511 4.0 6.00IE-49 AW731740.1 EST HUMAN ba55g05.xl NIH-MW_10 Horno saplens cIDNA done IMAGE:2900504 Y similar to gb:X17206 40S RIBOSOMAL PROTEIN S4 (HUMAN); gb:M20632 Mouse LI-Rep3 protein mRNA from a repetitive element, complete (MOUSE); 4935 10041 15113 1.0 6.OOE-49 AW801668.1 EST HUMAN IL5-UM0068-08040"57- clO UM0068 Homo sap ns 695 5855 10984 1.2 5.OOE-49 AL163210,2 NT Homo sapiens chromosome 21 segment HS21 C01 0 695 5850 10985 1.2 5.OOE-49 AL163210.2 NT Homo sapiens chromosome 21 segment HS21C010 1753 6875 12056 0.7 5,00E49 AA172121.1 EST HUMAN zp29c07.rl Stratagene neuroepithelium (#937231) Homo sapiens cIDNA done IMAGE:610860 Csimilarto TR:G233226 G233226 RWL-H PROTEIN.;contains LTR7A3 LTR7 LTR7 repetitive element:

2721 7805 13009 1.9 5.OOE-49 U17714.1 NT Homo saplens putative tumor suppressor ST13 (ST13) mRNA, complete ods 514 5678 10798 13.0 4.OOE-49 AW189533.1 EST-HUMAN A08b01.xl NCLWAP_W Homo sapiens cIDNA done IMAGE:2675593 Y similar to WPS0350.213 CE06703; 548 5711 10830 0.9 3.00E49 X68968.1 NT H.sapiens mRNA for acety]-CoA carboxylase 2 7701 0.7 3.00E49 AA016131.1 EST HU ze31c05xl Soares retina N2b4HR Homo sapiens cDNA clone IMAGE:360584 5' similar to -14 L1 repefitive'eiement; Page 103 of 196 Table 4

4909 10016 15096 2.1 3.OOE-49 U46999.1 NT Human type IV collagen (COL4A6) gene, exon 40 645 5804 0.9 2.OOE-49 BE165980.1 EST HUMAN MR3-HT0487-150200-113-901 HT0487 Homo sapiens cIDNA 3185 8334 13446 1.4 2.OOE-49 N26446.11 EST HUMAN yx23d06.M Soares meldnocyte 2NbHM Homo sapiens cDNA done I MAGE:262571 5' 3521 8662 13767 0.9 2.OOE-49 AF026564.1 NT Homo sapiens RNA binding protein 11 (RBMII) gene, complete cds 4693 9805 14905 1.1 2.OOE-49 AI 167357.1 EST HUMAN oz88d02.xl Soares- senescent fibroblasts NbHSF Homo sapiens cDNA done IMAGE:1682403 3' similar to qb:M31470 R -LIKE PROTEIN TC10 (HUMAN); containsAlu repefidve element;contains element MER22 repetitive element; 5286 104.13 0.6 1.OOE-49 4758135 NT Horno, sapiens DEAD/H (Asp-Glu-Ala- Asp/His) box polypeplide 11 (S.cerevisiae CHILI-like helicase) (DDX1 1) mRNA 5286 10414 0.6 1.OOE-49 4758135 NT Homo sapiens DEAD/H (Asp-Glu-AJa- Asp/His) box polypeplide 11 (S.cerevisiae CHL1-like helicase) (DDX1 1) mRNA 1760 6881 12063 0.9 11.0OE-49 BE255216.11 EST-HUMAN 601115769F1 N1H.MGC 16 Homo sapiens cDNA done IMAGE:3356273 5' 4923 10030 1.1 9.OOE-50 AF101475.1 NT Horno sapiens glydne N- thyltransferase (GNMT) gene, complete eds 163 5358 10483 0.9 8.OOE-50 A1.163202.2 NT Homo sapiens chromosome 21 segment HS21 C002 702 5857 10992 0.6 8.0OE-50 X95097.2 NT Horno sapiens mRNA for VIP receptor 2 702 5857 10993 0.6 8.OOE-50 X95097.2 NT Homo sapiens mRNA for VIP receptor 2 1016 6156 1.2 8.OOE-50 AF000573.1 NT Horno sapiens homogenfisate 1,2- dioxygenase gene. complete cds 1727 6850 12029 0.9 8.OOE-50 4501890 NT Homo sapiens acdnin, alpha 1 (ACTN1) mRNA 2324 7427 12637 1.1 8.OOE-50 Y16641.1 NT Rattus norvegicus mRNA for hnRNP protein, pardal 2432 7531 12736 1.4 8.OOE-50 7706394 NT Horno saplens p47 (LOC51674), mRNA 2432 7531 12737 1.4 8.OOE-50 7706394 NT Homo sapiens p47 (LOC51674), mRNA 2666 7753 12953 0.6 8.OOE-50 4826658 NT Homo sapiens capping protein (adin filament) muscle Z-Iine, beta (CAPZ13), mRNA 603 5761 10881 1.0 7.OOE-50 BE089591.1 EST-HUMAN QVO-BT0703-280400-21 1 - e08 BT0703 Homo sapiens cDNA 4002 9133 7-0.0 5.OOE-50 8922586 NT Homo sapiens hypothefical protein FLJ10675 (FLJ10675), mRNA 899 6046 1,3 4.OOE-50 AA601143.1 EST-HUMAN noS4e09.sl NCLWAP-SS1 Homo sapiens cDNA done IMAGE:1 104520 Ysimilar to gb:X53741 mall FIBULIN-1, ISOFORM A PRECURSOR (HUMAN); 1896 7013 0,9 3.OOE-50 M18048.1 NT Human endogenous retrovirus IRTW-H2 3259 8406 13513 1.1 3.00IE-50 AA746142.T EST HUMAN ob03f06.sl NCI CGAP K1d3 Homo sapiens cDNA done IMAGE:1322627 3' 3692 8831 13924 4.6 3.OOE-50 AW755254.1 EST-HUMAN CMYA5 Human cardiac muscle expression library Homo sapiens c[DNA clone 4151935 similar to CMYAS Cardiomyopathy associated gene 5 761 5913 1.7 2.OOE-50 AF055066.11 NT Horno, sapiens MI-IC class 1 region 1063 6201 11355 1.6 2.OOE-50 4557752 NT Horno, sapiens midline 1 (Opit7JBBB syndrome) (M1D1) mRNA 1425 6652 11729 6.5 2.OOE-50 AF138303.1 NT Horno sapiens decorin D mRNA, complete cds, altemadvely s liced 235 5426 10550 1.0 1.OOE-50 BE007080.11 EST-HUMAN PM3-BN011 37-290300-002- gl 1 BN01 37 Homo sapiens cIDNA 235 5426 10551 1.0 11.0OE-50 BE007080.1 EST-HUMAN PM3-BN011 37-290300-002- 911 BN01 37 Horno, sapiens cIDNA 454 5620 10747 0.7 1,OOE-50 ALI 63209.2 NT Homo, sapiens chromosome 21 segment HS21 C009 2314 7417 3.3 1.OOE-50 AJ271735.1 NT Homo sapiens Xq pseudoautosornal region; segment 112 1478 6605 8.2 9.0OE-511 Z12962.1 NT H.sapiens mRNA for homologue to yeast dbosomal protein L41 28 5237 10342 0,6 8.OOE-61 AA775999.1 EST HUMAN ae8291 0.sl Stratagene schizo brain S1 1 Horno sapiens cIDNA. done IMAGE:970722 3' 44 9587 14683 4.9 8.OOE-51 AA610842.1 EST HUMAN npg8e09.sl NCI - WAP Lul Homo sapiens cDNA done IMAGE:1 142440 Ysimilar to __---PZ 1 "1 - gb:X12671 mO HETEEOGENEOUS NUCLEAR RIBONUCLEOPROTEIN All (HUMAN); Page 104 of 196 Tabie 4 3245 8392 13499 1.4 7.OOE-51 AW889219.1 EST-HUMAN OV4-NT0028-200400-180- d05 NT0028 Homo sapiens cDNA xn34a03.xl NCI_PGAP Kidl 1 Homo sapiens cIDNA done IMAGE:2695564 3'similar to 3317 8462 13574 0.8 7.0OE-511 AW274720.1 EST HUMAN TR:C19D40 Q9Z340 A-fY- PICAL PKC SPECIFIC BINDING PROTEIN.; 3541 8682 13782 7.4 7.OOE-51 7662109 NT Homo sapiens KIAA0426 gene product (K1AA0426), mRNA WZp4341212229_rl 434 (synonym: htes3) Homo sapiens cIDNA clone WZp434132229 5' 4076 9203 14289 1.3 7.OOE-51 AL079628.1 EST HUMAN DKFZ034B2229j1 434 (synonym: htes3) Homo sapiens cDNA done WZp434B2229 Y 4076 9203 14290 1.3 7.OOE-51 AL079628.1 EST-HUMAN Ul-H-BWO-alp-b-05-0-Ul.sl NCI-CGAP-Sub(3 Homo sapiens cDNA done IMAGE:2729817 3' 4254 9379 14459 2.4 7.0OE-511 AW295603.1 EST-HUMAN Homo sapiens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog 1936 7053 12246 1.9 6.OOE-51 7657266 NT (K1AA0929), mRNA Homo sapiens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog 3428 13682 12.9 6.OOE-51 7657266 NT (K1AA0929), mRNA 8570 774 5926 11075 3.7 5.OOE-51 AL163203.2 NT Horno sapiens chromosome 21 segment HS21 C003 786 5937 11089.5 5.OOE-51 4507500 NT Homo sapiens T-wil lymphoma invasion and metastasis 1 (TIAM1) mRNA 975 7904 11277 1.4 5.OOE-51 AL-133204.1 NT Novel human gene mapping to chomosorne X 1590 6716 11886 0.8 5.0OE-511 5031980 NT Horno sapiens 26S proteasome- assodated padl hornolog (P0H1)

* mRNA 2548 76441 12843 4.2 5.OOE-51 A.1007558.1 NT Homo saplens mRNA for nudeoporin 155 3863 8999 14098 1.1 5.OOE-51 M30938.1 NT Human Ku (p701p80) subunit mRNA, complete cds 3863 895 14099 1.1 5.OOE-51 M30938.1 NT Human Ku (p701p80) subunit mRNA, complete cds 5004 10107 15175 2.3 5.OOE-51 A13037832.1 NT Homo sapiens mRNA for KIAA1411 protein, partial cds tr81c09.xl NCl-WAP_Paril Homo sapiens cIDNA done IMAGE:2224720 Ysimilar to 5326 10458 0.9 3.OOE-51 A1587348.1 EST-HUMAN gb:M26326 KERATIN, TYPE 1 CYTOSKELETAL 18 (HUMAN), zd76h04xl Soare!_fetat-heart-NbHHI9W Homo sapiens cDNA done IMAGE:346615 5' 613 5771 10891 0.6 3.OOE-51 W79058.1 EST HUMAN 114491i zd76h04xl Soares-fetat-heart-NbHHI9W Horno sapiens cIDNA clone IMAGE:346615 5' 613 5771 10892 0.6 3.OOE-51 W79058.1 EST HUMAN tr81 c09.xl NCI-CGAP_Panl Homo sapiens cDNA done IMAGE:2224720 3'similar to 1159 6294 1.4 3.OOE-51 A1587348.1 EST_HUMAN gb:M26326 KERATIN, TYPE 1 CYTOSKELETAL 18 (HUMAN); 4232 9356 14438 2.1 3.OOE-51 AL159142.1 NT Novel human gene mapping to chomosome 22 Horno sapiens ubiquifin protein ligase E3A (human papilloma virus E6- assodated protein, 362 5540 10671 0.6 2.OOE-51 4507798 NT Angelman syndrome) (UBWA) mRNA 673 5829 10956 1.1 2.OOE-51 BE391063.1 EST HUMAN 601285694F1 NIH MGC 44 Homo saplens cDNA done IMAGE:3607463 5' 673 5829 10957 1.1 2.OOE-51 BE391063.1 EST-HUMAN 601285694F1 NIH MGC 44 Homo sapiens cDNA done IMAGE:3607463 5 zr30a05xl Stratagene NT2 neuronal precursor 937230 Hbmo sapiens cIDNA done IMAGE:6W80 5'sImilar to TR:G233226 G233226 RTVL-H PROTEIN.;contains LTRM3 1662 6786 11963 0.8 2.OOE-51 AA233352.1 EST HUMAN LTR7 repetitive element; 36 8811 13905 2.2 2.OOE-51 W492415.1 EST-HUMAN U27903.xl NCI CGAP_Kidl 1 Homo sapiens cIDNA done IMAGE:2131732 3' U]-H-Bil-adj-d-02-0-Ul.sl NCI_PGAP-Sub21 Horno sapiens cIDNA, done IMAGE:2716851 3, 4 9,514 14603 1.0 2.OOE-51 AW137826,1 EST_HUMAN Page 105 of 196 Table 4

109 5312 10437 1.5 1.OOE-51 4503528 NT Homo sapiens eukaryofic translation initiation factor 4A, isoform 1 (E1F4A1) mRNA 4309 9432 14521 1.0 1.0OE-51 4759071 NT Homo sapiens small Inducible cytokine subfamily A (Cys-Cys), member 15 (SCYA15) mRNA 4309 9432 14522 1.0 1.OOE-51 4759071 NT Horno sapiens small Inducible cytokine s,ubfamily A (Cys-Cys). member 15 (SCYA1 5) mRNA 146 5342 10471 2.5 8.OOE-52 AA720574.1 EST-HUMAN nw21901s11 NCI CGAP- GWO Homo sapiens cIDNA done IMAGE: 1241138 3'sImilar to contains THR.t3 THR repetitive element; 1481 6608 11781 1,3 8.OOE-52 X84900.1 NT H.sapiens mRNA for laminin-5, alpha3b chain 1170 6304 0.9 6.OOE-52 BE072409.1 EST-HUMAN QV3-BT0537-271299-049-d07 BT0537 Homo sapiens cDNA 1668 6792 11968 0.8 6.OOE-52 AF109907.1 NT Homo saplens S164 gene, partial cds; PS1 and hypothetical protein genes, complete cds; and S171 gene, partial cds 751 5903 11049 0.6 5.0012-52 AW009208.1 EST HUMAN ws79a10.xl NCLWAP-Co3 Homo sapiens cDNA done IMAGE:2504154 Ysimilar to TR:043789 043789 OLFACTORY RECEPTOR; 751 5903 11050 0.6 5.OOE-52 AW009208.1 EST-HUMAN ws79a10.xl NCI - CGAP - Co3 Homo sapiens cDNA done IMAGE:2504154 3'similar to TR:043789 04i789 OLFACTORY RECEPTOR; 4340 9463 14553 2.1 5.OOE-52 Z78898.1 NT H.sapiens flow-sorted chromosome 6 Hindill fragment, SC pAl8H7 1639 6765 11934 0.9 4.OOE-52 AF257318.1 NT Homo sapiens SH3-containing protein SH3GLB1 mRNA, complete cds 1748 6870 12048 8.6 4.OOE-52 4758843 NT Homo sapiens nudeoporin 155ki) (NUP1 55) mRNA 2706 7791 20.5 4.OOE-52 AF047677.1 NT Homo sapiens dystrophin (DIVID) gene, deletion breakpoints 1-3 in Intron 5 3850 8986 14086 0.8 4.OOE-52 4507500 NT Horno, sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 577 5737 10853 1.5 3.OOE-52 Z20083.1 EST HUMAN HSAAA13JOK S, Human foetal Adrenals tissue Homo sapiens cIDNA 487 5652 10776 0.6 2.OOE-52 AA601246.1 EST-HUMAN no14909.sl NCI - CGAP - Phel Homo sapiens cDNA done IMAGE: 1100704 Ysimilar to TR:E239140 E239140 SPALT PROTEIN; 550 57T53 10831 1.4 2.OOE-52 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 550 5713 10832 1.4 2.OOE-52 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 2450 7549 12758 0.6 2.OOE-52 BE207575.1 EST-HUMAN bb66b07.yl N11-1-MGCJ Homo saplens cDNA done IMAGE:3030421 5'similar to gb:X16493 M.musculus mRNA for Zpf-1 Anc finger protein (MOUSE); 522 5686 10808 1.4 1.OOE-52 AA634445.1 EST HUMAN zu75h12.sl Soares tests.NHT Horno saplens cIDNA done IMAGE:743879 3' 1349 6477 11643 3.0 1.OOE-52 4504026 NT Homo sap ens glutamate-ammonia ligase (glutamine synthase) (GLUL) mIRNA 2489 7586 1.2 1.OOE-52 4502238 NT Homo saplens apAsulftase D (ARSD), transcript variant 1, mRNA 3021 8173 13273 1.4 1.OOE-52 S61070.1 NT pol=reverse transcriptase homolog (retroviral element) [human. endogenous retroviral element IRWL-Hpl, Genomic, 660 nt] 3723 8860 13952 1.0 9.OOE-53 4506064 NT Homo sapiens protein kinase, cAMP- dependent, regulatory, type 11, beta (PRKAR2B) mRNA 4293 9417 14507 1.0 9.OOE-53 AF001446.1 NT Horno sapiens core binding factor alphal subunit (C13FA1) gene, exon 3 4966 10069 15143 0.9 9.OOE-53 7661713 NT Homo sapiens predicted osteoblast protein (GS3786), mRNA 4009 9140 14222 2.2 5.OOE-53 4758543 NT Horno sapiens heterogeneous nuclear dbonudeoprotein C (Cl /C2) (HNRPC) mRNA 43 5253 10366 0.7 4.0012-53 AL163285.2 NT Homo, sapiens chromosome 21 segment HS21C085 43 5253 10367 0.7 4.OOE-53 AL163285.2 NT Homo saplens chromosome 21 segment HS21C085 47151 9827 14919 1.1 4.OOE-53 7705414 NT Homo saplens hookl protein (HOOK1), mRNA G 1 Page 106 of 196 Table 4

2625 7715 12916 0.9 3.OOE-53 A13026898.1 NT Horno saplens DNA, DLEC1 to ORCTL4 gene region, section 112 (DLEC1, ORCTO, ORCTL4 genes, complete cds) 3670 8809 13903 1.3 3.OOE-53 AW050836.1 EST-HUMAN wz22c07.xl ScaresDieckgraefe-colon-NHCD Horno, sapiens cDNA clone IMAGE:2558796 3 4486 9606 14699 1.2 3.OOE-53 AW803563.1 EST HUMAN IL2-UM0081-240300-055- DO3 UM0081 Horno sapiens cDNA 4833 9941 15030 0.9 3.OOE-53 BE069344.1 EST HUMAN QV3-BT0381-270100-073- d06 ST0381 Tionno saplens cDNA 450 5616 1.5 2.009-53 AA366556.1 EST-HUMAN EST77525 Pancreas tumor Ill Homo sapiens cDNA Cend 2279 7385 12592 1.1 2.OOE-53 U78027.1 NT Horno sapiens Bruton's tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like dbosomal protein (L441---)and FTP3 (FTP3) genes, complete cds 2490 7587 3.8 2.OOE-53 4502316 NT Homo sapiens ATPase, H+ transporting, lysosornal (vacuolar proton pump) 31 kD; Vacuolar proton-ATPase, subunit E; V-ATPase, subunit E (ATP6E), mRNA 2694 7781 12978 6.9 2.OOE-53 4757915 NT Homo sapiens core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D related (C13FA2T1) mRNA 2694 7781 12979 6.9 2.OOE-53 4757915 NT Horno sapiens core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D related (CFA2T1) mRNA 3207 8355 13465 1.2 2.OOE-53 AF083822.1 NT Homo sapiens dihydropyddine receptor alpha 2 subunit (CACNA2D1) gene, exon 6 3970 9102 14185 2.1 2.OOE-53 M61873.1 NT Human Krueppel-related DNA- binding protein (TIF34) gene, partial cds 4390 9512 14600 1.1 2.OOE-53 4506962 NT Homo sapiens SKAP55 homologue (SKAP-HOM) mRNA 5091 10189 15267 1.1 2.OOE-53 AL163281.2 NT Homo sapiens chromosome 21 segment HS21 C081 5091 10189 15268 1.1 2.OOE-53 AL1 63281.2 NT Horno, sapiens chromosome 21 segment HS21 C081 1429 6556 11732 0.9 1.OOE-53 AJ271736.1 NT Homo sapiens Xq pseudoautosomal region; segment 212 3364 8507 13615 1.0 1.OOE-53 AB026898.1 NT Homo sapiens DNA, DLEC1 to ORCTI.4 gene region, section 112 (DLEC1, ORCTI-3, ORCTL4 genes, complete cds) 5152 10247 15319 0.8 9.0012-54 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 5152 10247 15320 0.8 9.OOE-54 4507500 NT Horno sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 5394 10520 1.1 8.OOE-54 BE386785.1 ESTHUMAN 601272863F1 N1H MGC 20 Homo sapiens ONA, done IMAGE:3614031 5' 1794 6914 12100 1.3 8.OOE-54 4504610 NT Homo sapiens Insulin-like growth factor 2 receptor (IGF2R) mRNA 4629 9743 14839 1,3 8.OOE-54 4507848 NT Homo sapiens ubiquifin specific protease 13 (Isope tidase T-3) (USP13) mRNA 4629 9743 14840 1.3 8.OOE-54 4507848 NT Homo sapiens ubiquifin specific protease 13 (isopepfidase T-3) (USP13) mIRNA 5030 9743 14839 1.1 8.OOE-54 4507848 NT Homo sapiens ubiquifin specific protease 13 (isopepddase T-3) (USP13) mRNA 5030 9743 14840 1.1 8.OOE-54 4507848 NT Homc sapiens ubiquitin specific protease 13 (isopepfidase T-3) (USP1 3) mRNA 379 5585 10718 1.3 7.OOE-54 AA812537.1 EST-HUMAN a179c12.sl Soares - tesfis NHT Homo saplens cDNA done 1377046 Ysimilar to contains MER30A3 MER30 repedive element; 1789.6909 12095 0.6 7.OOE-54 Y16645.1 NT Horno sapiens mRNA for monocyte chemotactic p(otoin-2 2158 7267 12477 1.7 7.OOE-54 N27177.1 EST - HUMAN yw68d12.sl Soares_placentp_8togweeks-2NbHP8togW Homo, sapiens cDNA clone IMAGE:257399 Ysimilar to contains LTR713 LTR7 repetitive element; 22 5231 10334 0.5 6.OOE-54 AB003618.1 NT Homo sapiens DNA for MICB, exon 4, 5 and partial ods 1833 6952 12137 0.9 6.OOE-54 4505052 NT Homo saplens lymphocyte antigen 75 (LY75) mRNA, and translated products 1833 6952 12138 0.9 6.OOE-54 4505052 NT Horno sapiens lymphocyte antigen 75 (LY75) mRNA, and translated products 3246 8393 13500 0.8 6.OOE-54 8922148 NT Horno sapiens hypothetical protein DKFZp434M035 (DKIFZp434M035), mRNA 057 14144 2.4 6.OOE-54 4502872 NT Horno sapiens chloride channel 6 (CI- CNe) mIRNA Page 107 of 196 Tabie 4 C>Q G- 4774 9884 1.7 6.OOE-54 Y09846.1 NT H.saplens; shc eudogene, p66 Isoform 4930 9884 12425 1.3 6.OOE-54 Y09846.1 NT H.sapiens shc pseudogene, p66 isoform 2104 7215 0.8 5.OOE-54 P51523 SWISSPROT ZINC FINGER PROTEIN 84 (ZINC FINGER PROTEIN IrIPF2) 176 5369 36.0 4.OOE-54 AF110103.1 NT Tupaia belanged beta-actin mRNA, partial cds EST177696 Jurkat T-cells V1 Homo saplens cDNA V end similar to glyceraldehyde-3 940 6087 11244 14.0 4.OOE-54 AA306764.1 EST HUMAN phosphate dehydrogenase 1764 6885 12068 1.2 4.OOE-54 D38521.1 NT Human mRNA for KIAA0077 gene, pardal cds 1764 6885 12069 1.2 4.OOE-54 D385211.1 NT -.Human mRNA for KIAA0077 gene, pardalds Homo saplens cIDNA done IMAGE:2329269 3'similar 3168 8317 1.2 4.OOE-54 A1935086.1 EST HUMAN wd26dl 1.xl Soares- NFLT_GBC_Sl to TR:002711 002711 PRO-POL-DUTPASE POLYPROTEIN; cell line Homo sapiens cDNA E end 88 5294 10420 1.7 3.OOE-54 AA313487.1 EST HUMAN EST1 85371 Colon carcinoma (HCC) 2522 7618 12819 0.9 3.OOE-54 AL1 10383.1 EST-HUMAN DKFZp434E073,1_fl 434 (synonym: htes3) Homo sapiens cIDNA clone DKFZp434E0731 5' 2583 7676 10906 0.9 3.OOE-54 A1908757.1 EST-HUMAN IL-13T1 89-190399-007 BT1 89 Horno sapiens cDNA 626 5785 2.1 2.OOE-54 5031900 NT Homo saffiens killer cell lectin-like receptor subfamily G, member 1 (KI-RG1),. mRNA (SPIOO) mRNA 1342 6470 11637 0.7 2.OOE-54 4507164 NT Homo saplens nuclear antigen SplOO cDNA done IMAGE:1204600 similar to contains Horno nt78a09.s11 NCLWAP_Pr21 sapiens 1529 6656 11822: 0.6 2.OOE-54 AA655008.1 EST-HUMAN element L1 repetitive element; fetal brain 00004 Homo sapiens cIDNA done IMAGE:2783764 9 2493 7589 12793 1.3 2.OOE-54 AW163175.1 ESI_HUMAN au92g03.yl Schnelder 2556 7650 12853 0.7 2.OOE-54 AL.163210.2 NT similar to SW:W1-11_HUMAN Q13616 CULLIN HOMOLOG 1 Horno sapiens chromosome 21 segment HS21 C01 0 Homo sapiens cDNA clone 2865 8016 13119 1.7 2.OOE-54 AW057524.1 EST-HUMAN wy60bl 2.xl Soares- NSF_FUW_OT_PA-P-Si IMAGE:2552927 Ysimilar to TR:Q62084 Q62084 PHOSPHOLIPASE C NEIGHBORING; cIDNA clone IMAGE:99W8 similar to gb:X53777 Homo sapiens nj45g09.sl NCI---PGAP-Prg 3501 8643 14982 4.1 2.OOE-54 AA532925.1 EST_HUMAN 60S RIBOSOMAL PROTEIN L23 (HUMAN); 4110 9236 15322 2.0 2.OOE-54 4502642 NT Homo sapiens chaperonin containing T-complex subunit 6 (CCT6) mRNA 4343 9466.1.1 2.OOE-54 A1.163201.2 NT Homo saplens chromosome 21 segment HS21C001 4780 9890 1.5 2.OOE-54 7706446 NT Homo sapiens peptidylarginine delminase type Ill (LOC51702), mRNA 5156 10251 0.8 2.OOE-54 AF083823.1 NT Homo sapiens dlhydropyridine receptor alpha 2 subunit (CACNA2D1) gene, exon 7 1294 6423 14.6 8.0012-55 Y0782.9.2 NT Homo sapiens RFB30 gene for RING finger protein for RING protein 1297 6426 0.8 8.0OE-25 Y07829.2 NT finger Homo sa i-ens RFB30 gene 1 NFLS Homo sapiens cDNA done IMAGE:127998 5' liver yf26e04xl Soares fetal spleen 1065 620311358 1.2 7.OOE-55 R09346.11 EST HUMAN similar to SP:C561 BOVIN P10897 CYTOCHROME; Homo sapiens cDNA done IMAGE:462617 zj95b09.sl Soares-fetal-liveLspleen-1NFLS_S1 1732 6855 12032 1.1 5.OOE-55 AA704971.1 ESTHUMAN 3 zj95b09.s1 Soares-fetaLliver_spieen_l NFI-S_S1 Homo sapiens cDNA done IMAGE:462617 1732 6855 12033 1.1 5.OOE-55 AA704971.1 EST HUMAN 3' 49 7880 10375 1.4 4.OOE-55 AW957994.1 EST HUMAN EST370064 MAGE resequences, MAGE Homo sapiens cDNA Y chromosome, family 1, member AI (R13MY1 AI) motif Homo saplens RNA binding protein, 656 5813 10941 12.0 4,OOE-55 4826973 NT mRNA 0.7 4.0012-55 7661713 NT Homo sapiens predicted osteoblast protein (GS3786), mRNA Page 108 of 196 Table 4 s- s- 1422 6550 11726 0.7 4.OOE-55 7661713 NT Homo sapiens predicted osteoblast protein (GS3786), mRNA 1979 7094 12295 0.6 4.OOE-55 4506180 NT Homo saplens proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) mRNA 1979 7094 12296 0.6 4.OOE-55 4506180 NT Homo saplens proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) mRNA 2039 7152 12356 2.8 4.OOE-55 4503314 NT Homo saplens diacyiglycerol kinase, gamma (90k13) (DGKG) mRNA 2039 7152 12357 2.8 4.OOE-55 4503314 NT Homo sapiens diaWgiycerof kinase, gamma (9OkD) (DGKG) mRNA 2262 7368 12578 1.3 4.OOE-55 4W7794 NT Homo sapiens ubiquifin-conjugafing enzyme E2 variant 1 (UBE2V1) mRNA 2545 7641 1.0 4.OOE-55 A.1271735.1 NT Homo saplens Xq pseudoautosomal region; segment 112 3242 8389 13496 1.4 4.OOE-55 AL163300.2 NT Homo sapiens chromosome 21 segment HS21 Cl 00 373 5549 10682 0.8 2.OOE-55 X57147.1 NT Human endogenous retrovirus pHE.1 (ERV9) 539 5702 0.9 2.OOE-55 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 633 5792 10915 1.1 2.OOE-55 4507296 NT Homo sapiens syntayJn-binding protein 1 (STXB131) mIRNA, and translated products 1813 6932 12120 0.8 2.OOE-55 7019494 NT Homo sapiens putative protein 0mannosyltransferase (POMT2), mRNA 1813 6932 12121 0.8 2.OOE-55 7019494 NT Homo saplens putative protein 0- mannosyltransferase (POMT2), mIRNA 2928 8079 13188 0.8 2.OOE-55 4507798 NT Homo sapiens ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (U13E3A) mRNA 3141 8290 13396 2.9 2.OOE-55 BE070016.1 EST-HUMAN MR1-BT0405-080300-001- c04 BT0405 Homo sapiens cIDNA 3141 8290 13397 2.9 2.OOE-55 BE070016.1 EST-HUMAN MIRI-BT0405-080300-001- c04 ST0405 Homo sapiens cDNA 91 5297 10423 0.5 1.OOE-55 4505060 NT Homo saplens mannose-6-phosphate receptor (cation dependent) (M6PR) mRNA 182 5376 10501 4.1 1.OOE-55 U09823.1 NT Oryctolagus cuniculus New Zealand white elongation factor 1 alpha (Rabefla2) mRNA, complete cds 574 5734 1.4 1.0OE-55 A1905421.1 EST-HUMAN RC-OT091 -200199-072 BT091 Homo saplens cDNA 1132 626B 11420 1.2 1.0OE-55 AB020710.1 NT Homo saplens mRNA for KIAA0903 protein, partial ods 1907 7024 12212 0.9 1.OOE-55 BE277861.1 EST-HUMAN 601120116F1 N1H MGC_,RO Homo sapiens cIDNA clone IMAGE:2967027 W 19.07 7024 12213 0.9 1.0OE-55 BE277861.1 EST HUMAN 601120116F1 NIK_MG.20 Homo saplens cDNA done IMAGE:2967027 V 2277 7383 0.8 1.0OE-55 5803174NT Homo saplens SMA3 (SMA3), mRNA 2290 7873 12603 1.0 1.OOE-55 AF000990.1 NT Homo saplens tesds-spedfic Tesds Transcript Y 1 (TTY1) mRNA, partial ods 2470 7568 12770 12,0 1.OOE-55 AJ278305.1 NT Homo saplens HLA-A01 01 gene for M11C class 1 antigen, exons 1-8 2507 7603 12803 1.7 1.0OE-55 ABOO7866.2 NT Homo sapiens mIRNA for KIAA0406 protein, partial ods 2W7 7603 12804 1.7 1.OOE-55 ABOO7866.2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 2568 7661 12863 1.4 1.OOE-55 L54057.1 NT Homo sapiens CLI? mRNA, partial cds 2670 7757 12957 1.6 1.OOE-55 AW027307.1 ESTHUMAN wt73a10.xl Soares_thymus - NHIFTh Homo sapiens cDNA done IMAGE:2513082 Ysimilar to WRY47H9C.2 CE20263; 2670 7757 12958 1.6 1.OOE-55 AW027307.1 EST-HUMAN wt73a10.xl Soarejhymus- NHFTh Homo sapiens cDNA done IMAGE:2513082 Ysimilar to WRY47119C.2 CE20263; 3363 8506 13614 1.2 1.OOE-55 W28189.1 ESTHUMAN 43c5 Human refina cDNA randomly primed sublibrary Homo sapiens cDNA 3907 E3223 14129 3,5 1.OOE-55 AL163267.2 NT Homo saplens chromosome 21 segment H821 C067 4197 - 14410 1.0 1.OOE-55 AL163210.2 NT sapiens chromosome 21.segment HS21C010 47.21 9833 14924 1.0 1.0OE-55 AB037163.1 NT saplens DSCR5b mRNA, complete ods 47 9833 14925 1.0 1.0--- sapiens DSCR5b mRNA, complete ods; .......... 1 Page 109 of 196 Table 4

0 C 5081 10179 15259 1.2 1.OOE-55 8923125 NT Homo saplens hypothetical protein FLJ20126 (FI-J20126), mRNA 2703 7789 12989 1,9 7.OOE-56 H19934.1 EST HUMAN yn62g03xl Soares adult brain N2b51-1855Y Homo sapiens cDNA done IMAGE: 173044 5' similar to contains THR repetitive element; 4261 9386 14470 1.1 6.OOE-56 R96413.1 EST-HUMAN yq37e01xl Soares fetal liver spleen INFILS Homo sapiens cIDNA done IMAGE:197976 5' 1666 6790 11966 0.8 5.OOE-56 AW997712.1 EST-HUMAN RC3-BN0053-170200-011- h01 BN0053 Homo sapiens cDNA 5234 10337 2.1 4.0OE-56 AF141349.1 NT Homo sapiens beta-tubulin mRNA, complete cds 5234 10338 2.1 4.OOE-56 AF141349.1 NT Homo sapiens beta-tubulin mRNA, complete cds 2676 7763 12963 1.5 4.OOE-56 4507728 NT Homo saplens tubulin, beta polypepfide (TUBB) mRNA 2676 7763 12964 1.5 4.OOE-56 4507728 NT Homo sapiens tubulin, beta polypeptIde allBB) mIRNA 2781 5679 10800 1.0 4.OOE-56 AF003528.1 NT Homo sapiens X-linked anhidroldc ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 3138 8287 13394 1.1 4.OOE-56 AW293668.1 EST-HUMAN Ul-H-B]2-aho-d-07-0-Ul. sl NCLCGAP_Sub4 Homo sapiens cDNA clone IMAGE:2727588 3, 1318 6447 11613 9.9 3.OOE-56 8924029 NT Homo sapiens hypothetical protein PRO1304 (PRO1304), mRNA 2103 7214 12424 2.7 3.OOE-56 U-912697 NT Homo saplens oncogene TC21 (TC21), mRNA 3086 8237 13336 1.6 3.OOE-56 AA325826.1 EST HUMAN EST28889 Cerebellum 11 Homo saplens cDNA Send 3086 8237 13337 1.6 3.OOE-56 AA325826.1 ESI_HUMAN EST28889 Cerebellum 11 Homo sapiens cDNA Vend 3761 8899 1.4 3.0012-56 AF055066.1 NT Horno sapiens MI-IC class 1 region c 4327 9450 14538 4.1 3.0OE-56 AL-163268.2 NT Homo sa lens chromosome 21 segment HS21C068 4470 9590 14686 2.1 3.OOE-56 5902085 NT Homo sapiens superkiller viraliddic activity 2 (S. cerevisiae homologlike (SKIV21---),mRNA 513 5677 1.0 2.OOE-56 AA199818.1 EST-HUMAN zq52a08.sl Stratagene neuroepitheliurn (#937231) Homo sapiens cDNA clone IMAGE:645206 3' 716 7897 11008 1.2 2.OOE-56 BE064386.1 EST---HUMAN RC4wBT0310-110300-015- flO BT0310 Homo sapiens cIDNA.

716 7897 11009 1.2 2.OOE-56 BE064386.1 EST-HUMAN RC4-121T0310-110300015410 BT0310 Homo sapiens cDNA 2334 7437 12645 1.0 2.OOE-56 4585863 NT Homo sapiens phosphodlesterase 6A, cGMP-spedfic, rod, alpha (12DE6A), mRNA 2334 7437 12646 1.0 2.OOE-56 4585863 NT Homo sapiens phosphodlesterase 6A, cGMP-spedfic, rod, alpha (PDE6A), mRNA 2959 8110 13216 0.9 2.OOE-56 AB037835.1 NT Homo sapiens mRNA for KIAA1414 protein, partial cds 964 6109 1,4 1.0OE-56 AF190930.1 NT Macaca fascicularls protein tyrosine phosphatase (PRL-1) mRNA, complete cds 3622 8761 13853 1.8 1.OOE-56 AW589833.1 EST-HUMAN hg23cl 1.xl NCI CGAP GC6 Homo sapiens cDNA done IMAGE:2946452 3' 3622 8761 13854 1.8 1.OOE-56 AW589833.1 EST HUMAN h923cl 1.xl NCI CGAP GC6 Homo sapiens cDNA done IMAGE:2946452 3' 4963 10066 15138 1.5 1.OOE-56 A1905162.1 EST-HUMAN QV-BT077-130199-079 BT077 Homo sapiens cDNA 609 5767 0.7 9.0012-57 AW880885.1 EST HUMAN OVO-OT0033-070300-152-h03 OT0033 Homo sapiens cDNA 4109 9235 14318 1.2 9.OOE-57 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 4109 9235 14319 1.2 9.OOE-57 4758279 NT Homo, saplens EphA4 (IEPHA4) mRNA 13 5221 10322 0.5 8.OOE-57 8923349 NT Homo saplens hypoetical protein FLJ20371 (FL.12Q371), mRNA 294 5480 10611 1.0 8.0012-57 AW816405.1_ ESTHUMAN QV4-ST0234-181199-037- f05 ST0234 Homo saplens cDNA 866 6014 11174 2.0 8.OOE-57 AW264599.1 EST HUMAN xrO5d10.xl NCI-CGAP-Bm53 Homo sapiens cIDNA done IMAGE:2759251 Ysimilar to gb:U05875 INTERFERON-GAMMA RECEPTOR BETA CHAIN PRECURSOR (HUMAN); 177-A 6895 " 12083 0.6 8.OOE.57 AA496109.1 EST_HUMAN zv51b12.rl Soares testis NHT Horno sapiens cDNA done IMAGE:757151 5' Page 110 of 196 Table 4

A 3335 8479 13592 1.0 8.OOE-57 4758279 NT Homo sapi ns EphA4 (EPHA4) mRNA 3335 8479 13593 1.0 8.OOE-57 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 5107 10203 15282 1.0 8.OOE-57 AA971001.1 EST - HUMAN op67h02.sl Soares NFL T GBC S1 Homo sapiens cDNA done IMAGE:1 581939 3 2592 7684 12885 0.7 7.0012-57 7657592 NT Homo sapiens smg GDS-ASSOCIATED PROTEIN (SMAP), mRNA 2592 7684 12886 0.7 7.OOE-57 7657592 NT Homo sapiens smg GDS-ASSOCIATED PROTEIN (SMe), mRNA 3212 8360 13469 0,9 7.00IE-57 7242158 NT Horno, sapiens NME7 (NME7), mRNA 3212 8360 13470 0,9 7.OOE-57 7242158 NT Honio sapiens NME7 (NME7), mIRNA 3233 8381 13488 6.5 7.OOE-57 6005979 NT Homo sapiens Kruppel-like factor 8 (KLF8), mRNA 3801 8938 14032 2.2 7.OOE-57 AF012872.1 NT Homo sapiens phosphaddyll ositol 4-kinase 230 (p14K230) mRNA, complete cds 3801 8938 14033 2.2 7.OOE-57 AF012872.1 NT Homo saplens phosphaddylinositol 4-kinase 230 (p14K230) mRNA, complete cds 3694 8833 13925 1.2 4.OOE-57 AB026898.1 NT Homo saplens DNA, DLEC1 to ORCTL4 gene region, section 112 (DILEC1, ORCTI-3, ORCTL4 genes, complete cds) 787 -5938 11090 0.8 3.OOE-57 4507798 NT Homo sapiens ubiquidh protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (UBE3A) mRNA 1309 6437 3.9 3.OOE-57 AA230279.1 EST-HUMAN nc13f07.sl NCLWAP-Prl Homo sapiens cDNA done IMAGE:1008037 similar to SW:RS110 HUMAN P46783 40S RIBOSOMAL PROTEIN S1 0.

2342 7444 12651 1.0 3.OOE-57 AA348335.1 EST-HUMAN ESTS4770 Hippocampus 11 Horno, sapiens cDNA T end 3514 8655 13762 1.2 3.OOE-57 AF232708.1 NT Homo sapiens cell-line tsA201 a chloride ion current Inducer protein I(Cin) gene, complete cds 3639 8778 115.9 3.OOE-57 AW853964.1 ESTHUMAN IRC3-CT0254-110300-027-dl 0 CTO254 Homo sapiens cDNA 2699 7785 12985 1.2 2.OOE-57 AA845419,1 EST HUMAN ak02b02.sl Soaresparathyroid - tumoLNbHPA Homo sapiens cDNA done IMAGE:1404747 Ysimilar to contains Mu repetitive element;contains elem. ent MER22 repetitive element; 3392 8534 2.9 2.OOE-57 AL163204.2 NT Horno sapiens chromosome 21 segment HS21C004 3504 8646 13755 0,8 2.OOE-57 R07702.1 EST HUMAN ye98hO.l.rl Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:1258095' 3504 8646 13756 0.8 2.0012-57 R07702.1 EST HUMAN ye98h01.rl Soares fetal liver spleen 1NFI-S Homo sapiens cDNA done IMAGE:125809 W 4409 9529 14618 6.9 2.OOE-57 AL1163283.2 NT Homo sapiens chromosome 21 segment HS21C083 2184 7293 12505 1.1 11.0OE-57 AW503208.1 EST-HUMAN Ut-HF-BNO-akt-07-0-Ul. rl N11-1_1VIGC 50 Homo sapiens cDNA done IMAGE:3078348 5, 638 5797 10921 0.9 8.OOE-58 A1798376.1 EST-HUMAN tr34b07.xl NCI_PGAP - Ov23 Homo sapiens cDNA done IMAGE:2220181 Ysimilar to TR:015475 015475 UNNAMED HERV-H PROTEIN; 638 5797 10922 0.9 8.OOE-58 A1798376.1 EST-HUMAN tr34b07.xl NCI.. PGAP_jOv23 Homo sapiens cDNA done IMAGE:2220181 Ysimilar to TR:01 5475 015475 UNNAMED HERV-1-1 PROTEIN; 2945 8096 2.8 8.OOE-58 7706132 NT Horno sapiens DI-II-IC1 protein (LOC51304), mRNA 3382 8525 13631 4.2 7.OOE-58 BE263616.1 EST HUMAN 601192037F1 NIH MGC_7 Homo sapiens cDNA done IMAGE:3535977 5' 3382 8525 13632 4.2 7.OOE-58 BE263616.11 EST HUMAN 601192037F1 N11-11--1VIGC 7 Horno, sapiens cDNA done IMAGE:3535977 W 7316 12529 0.9 6.OOE-58 BE395061.1 EST-HUMAN 601309465F1 N11-1_1VIGQ_44 Homo sapiens cDNA done IMAGE:3631000 5' 8022 13124 1.2 6.OOE-58 BE242150.1 ESLI-IUMAN TCAA1P1 E1 219 Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA Homo sapiens cDNA done TCAAP1219 0 Page 111 of 196 Table 4 p 2871 8022 13125 1.2 6.OOE-58 BE242150.1 EST-HUMAN TCAAP1E1219 Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project--TCAA Homo saplens cDNA clone TCAAP1219 297 5483 10613 1.0 5.OOE-58 4507334 NT Homo sapiens synaptojanin 1 (SYNA), mRNA 1176 6310 11464 1.5 5.OOE-58 AW797M.1 EST-HUMAN C1V13-UM0043-240300-1127- e07 UM0043 Horno sapiens cDNA 1176 6310 11465 1.5 5.OOE-58 AW797948.1 EST-HUMAN CM3-UM0043-240300-127- e07 UM0043 Horno sapiens cDNA 1177 6310 11464 1.0 5.OOE-58 AW797948.1 EST-HUMAN CM3-UM0043-240300-127- e07 UM0043 Homo sapiens ciDNA 1177 6310 11465 1.0 5.OOE-58 AW7979487- EST-HUMAN CM3-UM0043-240300-1127- e07 UM0043 Homo sapiens cDNA 3281 8426 13534 3.6 5.OOE-58 AA988183.1 EST-HUMAN or98c07.sl NCI CGAP Lu5 Homo sapiens cDNA clone IMAGE:1603908 X 369 5547 10679 6.1 4.OOE-58 4502302 NT Horno sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, 0 subunit (oligomycin sensitivity conferring protein) (ATP50) mRNA 779 5931 11080 0.5 4.0OE-58 4504634 NT Homo sapiens Interleulkin 10 receptor, beta (11-11 ORB), mRNA 1452 6579 11753 1.2 4.OOE-58 4503W NT Homo saplens coagulation factor IX (plasma thromboplastic component, Christmas disease.

hemophilia B) (F9) mRNA 2531 7627 12829 1.0 4.0OE-58 AF265555.1 NT Homc, sapiens ubiquifin- conjugafing BIR-domain enzyme APOLLON mRNA, complete ods 2590 7683 12883 1.0 4.OOE-58 U36251.1 NT Human beta-prime-adaptin (BAM22) gene, exon 3 3283 8428 13536 1.1 4.OOE-58 D16470.1 NT Human mRNA, Xq terminal portion 3680 18819 13912 2.1 4.OOE-58 5031660 NT Horno saplens EG17-11ke repeats and discoldin 1-like domains 3 (EDIL3), mRNA 332 5513 1.2 3.OOE-58 R17879.1 EST HUMAN ygl 0e02.rl Soares Infant brain 1 NIB Horno sapiens cDNA done IMAGE:31693 5, 1367 6495 11661 0.9 3.OOE-58 4758981 NT Homo sapiens peptide YY (M) mRNA 925 6072 11230 2,8 2.OOE-58 AF068624.1 NT Horno saplens 5-aminolevulinate synthase 2 (ALAS2) gene, complete cds 1040 6180 11333 0.5 2.OOE-58 AW881145.1 EST HUMAN QVO-OT0033-010400-182- a07 OT0033 Horno sapiens cDNA 1268 6397 4.1 2.0012-58 BE208532.1 EST HUMAN ba08b0Tyl NIH-MGC-7 Homo sapiens cDNA done IMAGE:2823733 5'similar to gb:X69391 60S RIBOSOMAL PROTEIN L6 (HUMAN); gb:X81987 M.musculus mRNA for TAX responsive element binding protein (MOUSE); 706 5860 10995 0.9 11.0OE-58 M65134.1 NT Human complement component C5 mRNA, Yend 1052 6191 11343 1.9 1.OOE-58 6274549 NT Homo sapiens NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22k1D, B22) (NDUFB9), mRNA 1305 6434 11599 0.7 1.OOE-58 AW957182.1 EST-HUMAN EST369252 MAGE resequences, MAGI) Homo sapiens cIDNA 1305 6434 11600 0.7 1.OOE-58 AW957182.1 EST HUMAN EST369252 MAGE resequences, MAGI) Horno sapiens cDNA 1375 6503 11668 1.1 11.0OE-58 A.1238093.1 NT Homo sapiens partial AF-4 gene, exons 2 to 7 and Alu repeat elements 1641 6767 11938 2.0 11.0OE-58 BE466132.1 EST-HUMAN hylOf0,8.xl NCI CGAP GC6 Homc, saplens cDNA clone IMAGE:3196935 3' 2771 7855 13059 1.0 11.0OE-58 4759169 NT Homo saplens sterol regulatory element binding transcription factor 2 (SIREBIF2) mRNA 3493 8634 13747 1.0 11.0OE-58 4758081 NT Homo saplens chondroltin sulfate proteoglycan 2 (versican) (CSPG2 mRNA 3493 8634 13748 1.0 11.0012-58 4758081 NT Horno sapiens chondroifin sulfate proteoglycan 2 (versican) (CSPG2) mRNA 3659 8798 13892 0.8 11.0OE-58 4507628 NT Homo sapiens transition protein 1 (during histone to protamine replacement) (TNP1) mRNA 4627 9741 14838 0,9 11.0OE-58 M95963.11 NT Human prohormone converting enzyme (NEC2) gene, exon 4 4895 16002 15085 4.9 1.OOE-58 A1141063.1 EST-HUMAN oz43h011.xl Scares Nhi- IMPu S1 Homo sapiens cDNA cJone IMAGE:1678129 3' 2182 7291 12503 9.9 8.OOE-59 4507378 NT Homo sapiens TATA box binding protein (TBP) mRNA 3 8239 13339 6.2 5.OOE-59 A1807484.1 EST HUMAN - wf48c11 1.xl Scares NFL T GBC S1 Homc, sapiens cIDNA done IMAGE:2358836 3' Page 112 of 196 Table 4

3338 8482 3.9 5.OOE-59 ALI 59142.1 NT Novel human gene map ng to chomosome 22 4559 9677 14769 5.9 5.OOE-59 X83497.1 NT H.sapiens NA for ZW80-linked ERVg long terminal repeat 776 5928 11078 0.8 4.OOE-59 D80006.1 NT Human mRNA for KIAA01 84 gene, partial cds 4685 9797 14900 1.2 4.OOE-59 4506758 NT Horno saplens ryanodine receptor 3 (RYR3) mRNA 4685 9197 14901 1.2 4.OOE-59 4506758 NT Homo sapiens ryanodine receptor 3 (RYR3) mRNA 9 5217 2.0 3.OOE-59 AW965524.1 EST-HUMAN EST377582 MAGE resequences, MAGI Homo sapiens cDNA 221 5413 10534 1 41 3.OOE-59 7662247 NT Homo sapiens KIAA0680 gene product (K1AA0680), mRNA 1682 6806 11987 3.6 3.OOE-59 4505860 NT Horno sapi ns plasminogen activator, tissue (PLATa) mRNA 1682 6806 11988 3,6 3.OOE-59 4505860 NT Horno saplens plasminogen activator, tissue (PLATa) mRNA 2082 7194 12398 2.8 3.OOE-59 AF249745.1 NT Homo sapiens chromosome 2q22 RhoGEF mRNA, complete ods 2082 7194 12399 2.8 3.00IE-59 AF249745.1 NT Homo sapiens chromosome 2q22 RhoGEF mRNA, comp eettee s 3090 8241 13343 3 7i 3.00IE-59 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3090 8241 13344 3.7 3.OOE-59 4502014 NT Homo sapiens A kinase (PRKA)anchor protein 1 (AKAP1), mRNA 3753 8891 13981 1.2 3.00IE-59 4508044 NT Horno sapiens zona pelludda glycoprotein 2 (sperm receptor) (ZP2) mRNA 4683 9795 14898 1.1 3.OOE-59 4759329 NT Homo sapiens Testis-specific W- related protein on Y (XKRY) mRNA 4734 9845 14938 1.9 3.OOE-59 7427522 NT Homo sapiens protein tyrosine phosphatase, receptor type. T (PTPRT), mRNA 157 5353 1.2 1.OOE-59 BE29641 1.1 ESTHUMAN 601176757F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE:3531927 5' 2575 7668 0.9 1.0OE-59 AA748468.1 EST HUMAN oa56h11.sl NCI - WAP_GCS1 Homo sapiens cDNA clone IMAGE:1 309029 Ysimilar to TR:Q113537 013537 MER37 TRANSPOSABLE ELEMENT, COMPLETE CONSENSUS SEQUENCE.; 1455 6582 11756 1.0 8.OOE-60 4759159 NT Homo sapiens small nuclear ribonucleoproteln D3 polypeptide (1 OkD) (SNRPD3) mRNA 2125 7235 12443 1.0 8.OOE-60 5174656 NT Homo sapiens differentiation- related gene 1 (nickel-spedfic induction protein) (RTP) mRNA 2125 7235 12444 1.0 8.OOE-60 5174656 NT Homo sapiens differentiationrelated gene 1 (nickel-specific Induction protein) (RTP) mRNA 737 5891 11036 2.3 7.OOE-60 AF055066.1 NT Homo saplens WC. class 1 region 738 5891 11036 11.0 7.OOE-60 AF055066.1 NT Horno sapiens MI-IC class 1 region 798 5949 11099 1.2 7.0012-60 4504634 NT Homo sapiens intedeukin 10 receptor, beta (11-1 ORB), mRNA 2081 7193 12397 0.6 7.OOE-60 AF077188.1 NT Homo saplens cullin 4A (CUL4A) mRNA, complete ods 4088 9214 14297 2.6 7.OOE-60 4505488 NT Homo saplens omithine decarboxylase 1 (ODCI) mRNA 78 5284 10410 0.7 5.OOE-60 A1807917.1 EST-HUMAN wf52c07.xl Soares_NFL. T GBC S1 Homo sapiens cDNA clone IMAGE:2359212 3' 78 5284 10411 0.7 5.OOE-60 A1807917.1 EST HUMAN wf52c07.xl Soares_NFL T GBC S1 Homo sapiens cDNA done IMAGE:2359212 3 2188 7297 12511 0.9 4.OOE-60 AW503208.1 EST HUMAN Ul-HF-BNO-akt-g-07-0-Ul. rl N11-1 MGC. 50 Homo sapiens cDNA done IMAGE:3078348 5' 2188 7297 12512 0,9 4.00IE-60 AW503208.1 EST-HUMAN Ul-HF-BNO-akt-g-07-0- Ul.rl N1H MGC 50 Homo sapiens cDNA done IMAGE:3078348 5' 2942 8093 1.1 4.OOE-60 AA299037.1 EST HUMAN EST1 1498 Uterus Homo saplens cIDNA 5' end similar to similar to retrovirus-related pol 1826 6945 0.8 3.OOE-60 6031190 NT Homo sapiens prohibidn (PHB) mRNA 4354 9476 14567 2.1 3.OOE-60 A.1271735.1 NT Homo sapiens Xq pseudoautosomal region; segment 112 1405 653g 11703 1.0 2.OOE-60 Z1 1694.1 NT H.sapiens 41 kDa protein kinase related to rat ERK2 1 1691 6815 11994 1.3 2.OOE-60 M24603.1 NT Human bcr protein mRNA, Vend 3839 8975 14075 0.8 2.0012-60 AF231 919.1 NT Homo saplens chromosome 21 unknown mRNA V-,,, 5675 10796 0.5 1.OOE-60 BE178586.1 EST_HUMAN PM3-HT0605-270200-001- e06 HT0605 Homo saplens cIDNA Page 113 of 196 Table 4

It f 2739 7823 13027 0.7 1.0OE-60 L41825.1 NT Homo saplens CYP17 gene, Vend 4874 9981 15065 1.1 1.OOE-60 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 2635 7724 12927 1.4 8.OOE-61 AW006478.1 ESI-HUMAN wt05b10.xl NCI CGAPP93 Homo sapiens cDNA done IMAGE:2506555 3' 2635 7724 12928 1.4 8.OOE-61 AW006478.1 EST-HUMAN wtO5b10.xl NCI---WAP Co3 Homo sapiens cDNA clone IMAGE:2506555 3, 2921 8072 1,7 8.OOE-61 X57147.1 NT Human endogenous retrovirus pHE.1 (IERV9) 122 5320 10450 0.9 7.OOE-61 7706670 NT Homo sapiens PXR2b Rrotein (PXR2b), mRNA 122 5320 10451 0.9 7.OOE-61 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 123 5320 10450 0.9 7.OOE-61 7706670 NT Homo sapiens M2b protein (PXR2b), mRNA 123 5320.10451 0.9 7.OOE-61 7706670 NT Homo sapiens PX112b protein (PXR2b), mRNA 265 5452 10577 0.7 6.OOE-61 BE409310.1 ESTHUMAN 601300938F1 N1H MGC 21 Homo saplens cDNA done IMAGE:3635480 5' 794 5945 11096 0.6 6.OOE-61 BE4093110.1 EST HUMAN 601300938F1 N1H MGC 21 Homo saplens cIDNA done IMAGE:3635480 5' 1299 6428 11592 3.5 6.0OE-61 AF1 19860,1 NT Homo saplens PR02014 mRNA, complete cds 1612 6738 11908 1.0 6.OOE-61 BE257400.1 EST-HUMAN 601109238F1 NIH_MGC 16 Homo sapiens cDNA done IMAGE:3350145 5, 1628 6754 11924 0.8 6.OOE-61 AA596033.1 EST-HUMAN nn66h09.s1 NCI CGAP Larl Homo saplens cDNA done IMAGE:1 088897 3' 1655 6780 11953 0,6 5.OOE-61 4506008 NT Homo sapiens protein phosphatase 1, regulatory subunit 10 (PPP1R10) mRNA 3000 8152 13252 1.9 5.OOE-61 AL163279,2 NT Homo saplens chromosome 21 segment HS21 C079 3114 8263 13367 0.8 5.OOE-61 AB020632.1 NT Homo saplens mRNA for KIAA0825 protein, partial cds 3161 8310 13422 1.9 5.OOE-61 4502166 NT Homo saplens arnylold beta (A4) precursor protein (protease nexin-l I, Alzheirner disease) (APP), mRNA 3899 9034 1.7 5.OOE-61 AJ229041.1 NT Homo sapiens 959 kb contig between AMLI and CBRI on chromosome 21q22; segment 113 1434 6561 1.3 4.OOE-61 W28189.1 EST-HUMAN 43c5 Human retina cDNA randomly primed sublibrary Ho!!o sapiens cDNA 4119 9245 14329 1.1 3.0012-61 BE396279.1 EST HUMAN 601309785F1 N1H MGQ_44 Homo sapiens cIDNA done IMAGE:3631220 5, 490 5655 10778 045 2.OOE-61 8922829 NT Homo sapiens hypothetical protein FI-J1 1026 (FLJ1 1026), mRNA 1194 6326 11482 1.4 2.0012-61 BE168410.1 EST-HUMAN QV3-HT0513-060400-147- d01 HT0513 Homo sapiens cDNA 1194 6326 11483 1.4 2.0OE-61 BE166410.1 EST-HUMAN QV3-HT0513-060400-147- d01 HT0513 Homo sapiens cDNA 1642 6768 11939 1.3 2.OOE-61 N53039.1 EST-HUMAN yv53d11.sl Soares fetal liver spleen 1NIFILS Homo sapiens cDNA clone IMAGE:246453 3' similar to gb125444 60S RIBOSOMAL PROTEIN L35A (HUMAN); 2109 7220 12429 0,5 2.OOE-61 4758003 NT Homo sapiens calmegin (CLGN), mRNA 2604 7694 1.2 2.0012-61 BE384973.1 EST HUMAN 601276829F1 N11-1_1VIGC 20 Homo saplens cDNA done IMAGE:3617767 5' 428 5595 0.9 1.OOE-61 A1.163203.2 NT Homo saplens chromosome 21 segment HS21 C003 756 5908 11056 1.3 1.0OE-61 5453829 NT Homo sapiens origin recognition complex, subunit 2 (yeast homolog)4ike (ORC21---)mRNA 1377 6505 11670 1.1 1.OOE-61 AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 1731 6854 1.0 1.0OE-61 U32657.1 NT Human polymorphic trinudeofide repeat in X-linked refinifis pigmentosa (RP3) gene region 1814 6933 12122 1.6 1.0OE-61 6005983 NT Homo sapiens zona pelludda glycoprotein 3A (sperm receptor) (ZP3A), mRNA 2150 7260 12467 0.5 1.OOE-61 AW827281.1 ESTHUMAN xnl 1 b09.yl NCLCGAF_U5 Homo sapiens cDNA dons IMAGE:2693369 5'similar to contains element MSRI repetitive element; 2804 7956 13065 1.7 1.OOE-61 BE386363.1 EST HUMAN 601273513F1 N1H MGC 20 Homo saplens cDNA done IMAGE:3614667 5' 3 84 4 13587 0.9 1.OOE-61 7662319 NT Homo sapiens KIAA0806 gene product (K1AA0806), mRNA Page 114 of 196 Table 4

0 C 3671 8810 13904 1.5 1.00IE-61 BE174455.1 EST HUMAN QV2-HT0577-140300-077- 906 HT0577 Horno sapiens cl) 4339 9462 14551 1.0 1.OOE-61 4759249 NT Homo sapiens TRAF family member- associated NFKB activator (TANK) mRNA 4339 W2 14552 1.0 1.00IE-61 4759249 NT Homo sapiens TRAF family member- assodated NFKB activator (TANK) mRNA Ul-H-BWO-ajt-b-08-0-Ul.sl NCl-WAP-Sub(S Homo sapiens cIDNA clone IMAGE:2732871 3 4751 9862 14954 7,6 1.0OE-61 AW298181.1 EST HUMAN Ul-H-BWO-ajt-b-08-0-Ul.sl NCl-WAP_SuW Homo sapibns cIDNA clone IMAGE:2732871 3 4751 9862 14955 7.6 1.OOE-61 AW298181.1 ES1.HUMAN 4878 9985 15067 0,9 1.OOE-61 AL163210.2 NT Homo saplens chromos me 21 segment HS21C010 oc66hl I.sl NCI-CGAF_GCBI Homo sapiens cDNA clone IMAGE:1354725 Ysimilar to 4451 9571 14670 0.8 8.00IE-62 AA830420.1 EST HUMAN SW:POL IVILVIRK P31795 POL POLYPROTEIN; NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) 3462 8603 13716 0.8 7.OOE-62 P17480 SWISSPROT (AUTOANTIGEN NOR-90) 2969 8120 1.1 6.OOE-62 U09410.1 NT Human zinc finger protein ZNFI 31 mRNA, partial cds wx51c07.xl NCI_PGAP_Lu28 Homo sapiens cDNA done IMAGE:2547204 Ysimilar to EST-HUMAN SW:GG95 HUMAN Q08379 GOLGIN-95.;contains element MER22 repetitive element; 410 5578 10715 1.5 5.OOE-62 A1950528.1 2356 7458 12664 1.2 5.OOE-62 AJ271735.1 NT Homo sapiens Xq pseudoautosomal region; segment 112 2356 7458 12665 1.2 5.0012-62 A.1271735.1 NT Homo sapiens Xq pseudoautosomal regio; segment 112 3372 8515 13622 2.2 5.OOE-62 4506758 NT Homo sapiens ryanodine receptor 3 (RYR3) mRNA zw78e09.sl Soares-tesds-NHT Homo sapiens cDNA done IMAGE:782344 3'similar to 4233 9357 14439 1.7 5.OOE-62 AA431093.1 EST-HUMAN SW:1SIRDC RAT P47245 NARDILYSIN; au71d03.yl Schnelder fetal brain 00004 Homo sapiens cDNA done IMAGE:2781701 W similar to gb:M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 822 5972 11129 1.2 4.OOE-62 AW161479.1 EST HUMAN PRECURSOR(HUMAN1); au71c103.yl Schneider fetal brain 00004 Homo sapiens cDNA done IMAGE:2781701 5, similar to gb:M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 822 5972 11130 1.2 4.OOE-62 AW161479.1 EST-HUMAN PRECURSOR(HUMAN); au71d03.yl Schnelder fetal brain 00004 Horno sapiens cDNA done IMAGE:2781701 5, similar to gb.M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 823 5972 11129 1.6 4.OOE-62 AW161479.1 EST-HUMAN PRECURSOR(HUMAN); au71d03.yl Schnelder fetal brain 00004 Homo sapiens cDNA done IMAGE:2781701 5, similar to qb:M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 823 5972 11130 1.6 4.OOE-62 AW161479.1 EST HUMAN PRECURSOR(HUMAN); 1446 6573 0.9 4.00IE-62 AA311281.1 EST-HUMAN EST182043 Jurkat T-cells V Homo sapiens cDNA Vend wf12b08.xl SoaresNFLGBC-Sl Homo sapiens cDNA done IMAGE:2350359 Ysimilar 2409 7508 12715 1.4 4.OOE-62 A1827900.1 EST_HUMAN to gb:X57138-mal HISTONE H213.2 (HUMAN); wf12b08.xl SoaresYsimilalr _NFL - T - GBC: - S1 Homo sapiens cDNA clone IMAGE:2350359 Ysimilar 2409 7508 12716 1.4 4.OOE-62 A1827900.1 EST-HUMAN to gb:X5713_rnal HISTONE H2B.2 (HUMAN); 2821 7972 13074 1.1 4.OOE-62 L10391.1 NT Rabbit flaAn-containing monooxygenase FM03 mRNA, complete cds 3353 8497 6.6 4.00IE-62 4557887 NT Homo saplens keratin 18 (KIRT18) mRNA 4947 10052 2.0 4.OOE-62 AJ243213.1 Homo sapiens partial 5-HT4 receptor gene, exons 2 to 5 8 5275 10400 E-62 4557794 NT Homo sapiens neurofibromin 2 (bilateral acoustic neuroma) (NF2) mRNA Page 115 of 196 Table 4

3008 8160' 13260 1.0 3.OOE-62 AB040909.1 NT Horno saplens mRNA for KIAA1476 protein, partial cds 3008 8160 13261 1.0 3.OOE-62 AB040909.1 NT Horno saplens mRNA for KIAA1476 protein, partial cds 3640 8779 13872 1.9 3.OOE-62 X52858.11 NT Human cydophilin-r&ated processed pseudogene 1211 6342 11501 0.5 2.OOE-62'ZL-163284.2 NT Homo sapiens chromosome 21 segment HS21C084 1028 6168 11323 1,2 11.0OE-62 AF248540.1 NT Homo sapiens intersectin 2 (SH3D1 B) mRNA, complete ods 1526 6653 11820 2.5 1.0OE-62 L7881 0.1 NT Homo saplens ADIRIATIP carder protein (ANT-2) gene, complete ods 1758 6879 12060 1.0 1.OOE-62 AA625207.11 EST-HUMAN af7Oell.rl Scares NhHIVIPu-SI Homo sapiens cIDNA done IMAGE:1047404 5'similar to WRIKO1H12.1 CE03453; 2884 8035 13138 1.1 11.0OE-62 AL039044,1 EST-HUMAN DIKIFZp5661F11 04-rl 566 (synonym: hfKd2) Homo sapiens cIDNA. clone DIKFZp566F1 04 5' 4426 9547 14640 1.3 11.0OE-62. 8923201 NT Homo sapiens hypothetical protein FLJ20212 (FL.1202112), mIRNA.

335 5516 10641 0.7 9.OOE-63 AW816405.1 EST - HUMAN QV4-ST0234.181199-037f05 ST0234 Homo sapiens cDNA 2297 7401 0.6 9.OOE-63 C18159.1 EST HUMAN Cl 8159 Human placenta cIDNA (TFujiwara) Homo saplens cIDNA done GEN-558C1 0 5' 3955 9088 14170 7,4 9.OOE-63 ABOO2348.2 NT Homo sapiens mRNA for KIAA0350 protein, partial ods 3955 9088 14171 7.4 9.OOE-63 AB002348.2 NT Homo sapiens mRNA for KIAA0350 protein, partial ods 2296 7400 12609 0.6 8.OOE-63 4557734 NT Homo saplens monoamine oxidase A (MAOA), nuclear gene encoding mitochondrial protein, mRNA 2322 7425 12634 0.8 8.OOE-63 5031810 NT Homo sapiens. 112-inducible T- cell kinase (ITK), mIRNA 3415 8557 13666 3.8 8.OOE-63 AF198349,1 NT Gallus gallus Dach2 protein (Dach2) mIRNA, complete cds 3415 8557 13667 3.8 8.OOE-63 AF198349.1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete eds 4168 9294 14375 3.4 8.OOE-63 AL163268.2 NT Homo sapipris chromosome 21 segment HS21C068 913 6059 0,6 7.OOE-63 A1872137.1 EST HUMAN wm55gl 1.x NCI CGAP W2 Homo sapiens cDNA clone IMAGE:2439908 3' 3279 8424 13533 1.0 4.OOE-63 AL163278.2 NT Homo sapiens chromosome 21 segment HS21C078 1893 7010 12198 0.7 3.OOE-63 AB018260.1 NT Homo sapiens mRNA for KIAA0717 protein, partial cds 2753 7837 13038 1.3 3.OOE-63 J003110.1 NT Human Met-tRNA-i gene 1 2791 634911510 3.1 3.OOE-63 6005963 NT Homo saplens zinc finger protein 144 (Mel-18) (ZNF144), mIRNA 4445 E65 14661 3.2 3.0012-63 AW954734.1 EST-HUMAN EST366804 MAGE resequences, MAGC Horno sapiens cDNA 184 5378 10504 1.1 2.OOE-63 U07804.11 NT Human DNA topoisomerase 1 mRNA, partial cds 191 5385 10512 0.6 2.OOE-63 4885226 NT Homo sapiens eyes absent (Drosophila) homolog 2 (EYA2), mRNA 489 5654 1.4 2.OOE-63 4557624 NT Homo sapiens glutamate-cystelne ligase (gamma-glutamytcysteine synthetase), catalytic (72.8kID) (GLCLC) mRNA 809 5960.11115 1.8 2.0012-63 7657042 NT Homo sapiens Down syndrome candidate region 1 (DSCR1), MRNA 1547 6674 11844 0.9 2.OOE-63 AB030388.1 NT Homo sapiens R14CE mIRNA for Rh blood CE group anfigen Rolypepfide, complete cds 1547 6674 11845 0.9 2.OOE-63 AB030388.1 NT Homo sapiens RHCE mRNA for Rh blood CE group antigen polypepfide, complete cds 3119 8268 13373 1.7 2.OOE-63 4502166 NT Homo saplens arnyloid beta (A4) precursor protein (protease nexJn-li, Alzheirner disease) (APP), mRNA 3248 8395 13502 1.8 2.0OE-63 AF1097118A NT Homo sapiens chromosome 3 subtelomeric region 3831 8968 14067 1.9 2.OOE-63 L39891.1 NT Homo saplens polycystic kidney disease-associated protein (PKDI) gene, complete ods 47 1 14966 1.1 2.OOE-63 AF1 11 1672 NT Homo saplens jun dimedzadQn protein gene, partial ods; cfos gene, complete cds, and 9871 unknown gene Page 116 of 196 Table 4

4246 9371 14450 2.9 1.0OE-63 F08485.1 EST HUMAN H8WVD1 11 normalized infant brain cDNA Homo sapiens cIDNA done c-zvdl 1 4246 9371 14451 2,9 1.OOE-63 F08485.1 EST HUMAN H=VID1 11 normalized Infant brain cIDNA Homo sapiens cIDNA done c-zvdl 1 1307 6436 11602 0.6 9.OOE-64 H30687.1 EST HUMAN yo78c02xl Scares adult brain N2b41-IB55Y Homo sapiens cIDNA clone IMAGE: 184034 5' 1307 6436 11603 0.6 9.OOE-64 H30687.1 ESTHUMAN yo78c02.rl Soares adult brain N2b41-11355Y Homo saplens cDNA done IMAGE:184034 5' 1030 6170 2.7 8.OOE-64 BE280796.1 EST HUMAN 601155232F1 NIH MGC 21 Homo sapiens cDNA done IMAGE:3139038 5 4874 97U6 5.2 8.OOE-64 AL1632133 NT Horno saplens chromosome 21 segment HS21 COl 3 3486 8627 1.0 7.OOE-64 BE394321.1 EST-HUMAN 601311455F1 NIH MGC 44 Horno sapiens cIDNA clone IMAGE:3633204 5' 4625 9739 14835. 2.4 7.OOE-64 4507490 NT Homo sapiens thimet ofigopepfidase 1 (THOP1) mRNA 4625 9739 14836 2.4 7.OOE-64 4507490 NT Homo sapiens thimet oligopepfidase 1 (THOP1) mRNA 1692 6816 11995 0.6 6.OOE-64 A1651992.1 EST-HUMAN wb51e07.xl NCI - CGAP - GC6 Homo saplens cDNA done IMAGE:2309220 Ysimilar to gb:MI 5182 BETA-GLUCURONIDASE PRECURSOR (HUMAN), 1692 6816 11996 0.6 6.OOE-64 A1651992.1 EST HUMAN wb51e07.xl NCI - WAP_GC6 Homc, saplens cIDNA done IMAGE:2309220 3' similar to gb:M15182 BETA-GLUCURONIDASE PRECURSOR (HUMAN); 2675 7762 12962 1.1 6.OOE-64 BE252937.1 EST HUMAN 601117525F1 NIH MGC 16 Homo saplens cDNA done IMAGE.3357966 V 3084 8235 13333 3.7 6.OOE-64 AW026445.1 EST-HUMAN wv13e03.xl NCI CGAP Bm23 Homo saplens cIDNA done IMAGE:2529436 3' 3084 8235 13334 3,7 6.OOE-64 AW026445.1 EST-HUMAN w03e03.xl NCI CGAP Bm23 Homo sapiens cDNA done IMAGE:2529436 3' 803 5954 11106 0.8 5.OOE-64 AF231919.1 NT Homo saplens chromosome 21 unknown mRNA 803 5954 11107 0.8 5.OOE-64 AF231919.1 NT Homo saplens chromosome 21 unknown mRNA 1402 652 11699 0.9 5.0012-64 L40933.1 NT Homo saplens phosphoglucomutase- retated protein (PG1VIRP) gene, complete cds 1402 6529 11700 0.9 5.OOE-64 L40933.1 NT Homo sapiens phosphoglucomutase- related protein (PGMRP) gene, complete cds 1683 6807 11989 0,6 5.OOE-64 U89358.1 NT Human 1(3)mbt protein homolog mRNA, complete cds 2796 6592 11767 1.0 5.0OE-64 7662205 NT Homo saplens KIAA0618 gene product (K1AA0618), mRNA 2796 6592 11768 1.0 5.OOE-64 7662205 NT Homo sapiens KIAA0618 gene product (K1AA0618), mRNA 3876 9012 14107 5.6 5.OOE-64 AF017433.1 NT Homo saplens putative transcription factor CR53 (CR53) mRNA, partial cds 2151 7261 12468 1.4 3.OOE-64 C18895.1 EST-HUMAN Cl 8895 Human placenta cDNA (TFuJIwara) Homo saplens cIDNA done GEN-569E02 5' 1072 6210 11363 0.9 2.OOE-64 AA609940.1 EST HUMAN afOgdO8.sl Scares testis NHT Homo saplens cDNA done IMAGE:1031151 Y.

1376 6504 11669 1.3 2.OOE-64 4757701 NT Horno saplens e1F4E-like capbinding protein (4EHP) mRNA 2478 7576 0.6 2.OOE-64 A1927030.1 EST-HUMAN wo87b01.xl NCI_CGAP_Kidl 1 Homo sapiens cIDNA done IMAGE:2462281 Ysimilar to contains element Ll repetitive element; 2484 7581 12784 1.1 2.OOE-64 AL163246.2 NT Homo sapiens chromosome 21 segment HS21C046 2484 7581 12785 1.1 2.OOE-64 AL163246.2 NT Horno saplens chromosome 21 segment H821C046 3101 8251 13355 2.6 2.0OE-64 4504068 NT Homo sapiens glutamic-oxaloacedc transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochonddal protein, mRNA 3719 8856 13948 1.3 2.OOE-64 AW958145.1 EST HUMAN EST370215 MAGE resequences, MAGE,Homo sapiens cDNA 3719 8856 13949 1.3 2.OOE-64 AW958145.1 ESTHUMA EST370215 MAGE resequences, MAGE Homo sapiens cIDNA 258 5446 10571 0.6 1.OOE-64 AF231 919.1 NT Horno saplens chromosome 21 unknown mRNA 17041, 6863 12040 2.1 1.OOE-64 A1929419.1 EST_HUMAN au60c01.xl Schnelder fetal brain 00004 Homo sap!ens ZJNA done IMAGE:2519136 3' similar to gb:L21696_,pdsl PROTHYMOSIN ALPHA (HUMAN);contains element MSR1 repetitive element; Page 117 of 196 Table 4

1;O 10 r 3466 8607 13720 5.6 1.OOE-64 AF196779.1 NT Homo sapiens transcription factor IGHM enhancer 3, M1 1 protein, JM4 protein, M5 protein, T54 protein, JM10 protein, A4 differentlation-dependent protein, triple LIM domain protein 6.

and synaptophysin genes, complete ods; and L-type calcium channel a> 3536 8677 13780 1.3 1.0OE-64 AF228527.1 NT Homo sapiens TRIAD3 mRNA, partial ods 3536 8677 13781 1.3 1.OOE-64 AF228527.1 NT Homo sapiens TRIAD3 mRNA, partial cds 4142 9268 2.7 1.OOE-64 8922772 NT Homo sapiens hyRothefical protein FLJ10923 (FLA0923), mRNA 1598 6725 11895 0.5 9.OOE-.65 8922772 NT Homo sapiens hypothetical protein FLJ10923 (FLA0923), mRNA 2230 7337 12549 0.9 9.OOE-65 X8921 1,1 NT H.sapiens DNA for endogenous retroviral like element 2230 7337 12550 0.9 9.OOE-65 X8921 1.1 NT H.sapiens DNA for endogenous retroviral like element 1880 6998 3.0 6.0OE-65 AA550929.1 ESTHUMAN nj86d1O.C NCI CGAP - PM 1 Homo sapiens cDNA done IMAGE:999379 similar to 9M03002 60S RIBOSOMAL PROTEIN L32 (HUMAN); 615 5773 10894 0.9 5.OOE-65 AF064604.1 NT Homo sapiens KE03 protein mRNA, partial cds ' 1331 6460 11626 0.6 5.OOE-65 7661951 NT Homo sapiens KIAA01 56 gene product (K1AA011 56), mRNA 1331 6460 11627 0.6 5.OOE-65 7661951 NT Homo saplens KIAA0156 gene product (K1AA0156), mRNA 2110 7221 12430 1.0 5.0015-65 A13033768.1 NT Homo saplens WAD-colony10 mRNA for pepddylarginine deiminase type 1, complete cds 2336 7438 12648 1.0 5.OOE-65 BE311673.1 EST-HUMAN 601142861 F1 N1H MGC 14 Homo sapiens cDNA done IMAGE:3506774 5' 3217 8365 13476 1.9 5.OOE-65 4507848 NT Homo sapiens ubiquign specific protease 13 (isopeptIdase T-3) (USP1 3) mRNA 3217 8365 13477 1.9 5.OOE-65 4507848 NT Homo saplens ubJquidn specific protease 13 (isopepfidase T-3) (USP13) mRNA 186 5380 10507 1,1 4.OOE-65 AL120419.1 EST-HUMAN DKFZp761 G1 08-rl 761 (synonym: hamy2) Homo sapiens cDNA clone DKFZp761 G1 08 5, 728 5882 11023 0.5 4.OOE-65 A1266468.1 EST-HUMAN qm46e01.xl Soares_placenta_8togweeks-2NbHP8togWlHomo sapiens cDNA done IMAGE:1891800 3' 728 5882 11024 0.5 4.OOE-65 A1266468.1 EST-HUMAN qm46e01.xl Scares_piacenta_8togweek!_2NbHP8togW Homo sapiens cDNA clone IMAGE:1891800 3' 1062 6200 11354 0.6 4.OOE-65 4826735 NT Homo sapiens fragile X mental retardation, autosomal homolog 1 (FXR-ii,' MIRNA 1470 6597 11772 3.0 4.OOE-65 4506636 NT Horno sapiens dbosomal Rrotein L34 (RPL34) mRNA 2288 7393 12600 1.0 4.OOE-65 BE221469.1 EST-HUMAN hu25e04.xl NCI CGAP Me115 Homo sapiens cDNA clone IMAGE:3171102 3' 2288 7393 12601 1.0 4.OOE-65 BE221469.1 EST-HUMAN hu25c04.xl NCI CGAP Me115 Homo sapiens cDNA done IMAGE:3171102 X 5138 10233 15307 1.0 4.OOE-65 9055269 NT Homo sapiens low density llpoproteln receptor related protein-deleted in tumor (LRPDIT), mRNA 5138 10233 15308 1.0 4.OOE-65 9055269 NT Homo sapiens low density lipoprotein receptor related protein-deleted In tumor (LRPDIT), mRNA 121 2 7868 1.8 3.OOE-65 X78932,1 NT H.sapiens HW9 mRNA for zInc finger protein U 6901 12089 1.1 3.OOE-65 A1000692.1 EST-HUMAN ov23f03.sl Soare!_tesds_NHT Homo sapiens cDNA clone IMAGE:1 638173 Ysimilar to contains element MSR1 repetitive element; 3239 8386 13494 1.4 3.OOE-65 4504950 NT Horno, sapiens laminin, beta 1 (LAMB1), mRNA 3660 8799 13893 1.0 3.OOE-65 A1000692.1 EST HUMAN ov23f01s11 Soares tesds_NHT Homo sapiens cDNA done IMAGE:1638173 3, similar to contains element hiSR1 repetitive element; C-, I Page 118 of 196 Table 4

4551 9670 14760 1.4 3.OOE-65 6912385 NT Homo sapiens rab6 GTPase activating protein (GAP and centrosome-associated) (GAPCENA). mRNA 528 5692 10814 1.3 1.0OE-65 7657495 NT Homo sapiens putative Rab5 GIDPIGTP exchange factor homologue (RABEX5), mRNA 1994 7110 12313 0.9 1.OOE-65 A13040946.1 NT Homo sapiens mRNA for KIAA1513 protein, partial ods 3326 8470 13584 0.9 1.OOE-65 BE466681.1 EST-HUMAN hz24a09.xl NCI CGAP GC6 Homo sapiens cDNA done IMAGE:3208888 3' 3917 9051 14138 2.1 1.OOE-65 4504082 NT Homo sapiens glypican 4 (GPC4) mRNA 3917 9051 14139 2.1 1.OOE-65 4504082 NT Homo sapiens glypican 4 (G12C4) mIRNA 4112 9238 14321 2.4 1.OOE-65 AW029340.1 EST HUMAN wxOgcOg.xl NCI CGAP Gas4 Homo saplens cDNA clone IMAGE:2543152 3' 4112 9238 14322 2.4 1.0OE-65 AW029340.1 ESTHUMAN wxOgcOg.xl NCI CGAP Gas4 Horno saplens cIDNA done IMAGE:2543152 3' 4415 9535 14624 1.1 1.0012-65 AW136725.1 EST HUMAN Ul-H-Bill-adm-a-1 1-0- Ul.sl NCI-CGAF_Sub3 Homo sapiens cIDNA clone IMAGE:2717108 3' 4415 9535 14625 1.1 1.OOE-65 AW136725.1 EST-HUMAN Ul-H-Bil-adm-a-11-0-Ul. sl NCI-CGAP-Sub3 Homo sapiens cDNA done IMAGE:2717108 3, 5273 10396 0.5 9.OOE-66 AL1 60311.1 NT Novel human gene mapping to chomosorne 22 5273 10397 0,5 9.OOE-66 AL1 60311.1 NT Novel human gene mapping to chomosome 22 1332 6461 11628 0.9 9.OOE-66 5031980 NT Homo sapiens 26S proteasomeassoclated padi homolog (P0H1) mRNA 1332 6461 11629 0.9 9.OOE-66 5031980 NT Homo saplens 26S proteasome- associated padl homolog (P0H1) mRNA 1468 6595 1.5 9.OOE-66 M87299.1 NT Human transposon-like element, partial 2480 7578 12779 1.0 9.OOE-66 8923943 NT Homo sapiens transcription factor NIRIF (NRF), mRNA 2480 7578 12780 1.0 9.OOE-66 8923943 NT Homo saplens transcription factor NRIF (NRF), mRNA 3826 8963 14061 0.9 9.0012-66 M72393.1 NT Human caldum-dependent phospholipid-binding protein (PLA2) mRNA, complete cds 3826 8963 14062 0,9 9.OOE-66 M72393.11 NT Human caldum-dependent phospholipid-binding protein (PLA2) mRNA, complete ods 4591 9705 14798 0.9 8.OOE-66 AA424304.1 EST HUMAN zvgOoD5.rl Soares NhiIMPu SI Homo saplens cDNA done IMAGE:767048 9 4269 9394 14476 1.2 6.OOE-66 A1924653.1 EST-HUMAN wn57h07.xl NCI-CGAPJ1- ull g Homo sapiens cDNA done IMAGE:2449597 3'sImilar to WRF15G9.4ACE18595; 4269 9394 14477 1,2 6.OOE-66 A1924653.1 EST-HUMAN wn57h07.xl NCLWAP-Lu.19 Homo sapiens cDNA done IMAGE:2449597 Ysimilar to WRF115G9.4A CE118595; 4269 9394 14478 1.2 6.OOE-66 A1924653.1 EST HUMAN wn57h07.xl NCI_CGAP_Lulg Homo sapiens cIDNA done IMAGE,2449597 5'sImilar to WRIFI5G9.4A CE18595; 1344 6472 11639 0.8 5.OOE-66 BE064410.1 EST HUMAN RC4-13T0311-1411199-01 1 -h06 BT0311 Homo sapiens cDNA 773 5925 11074 0.8 4.OOE-66 6679816 NT Mus musculus fragile X mental retardation syndrome 1 homolog (Fmrl), mRNA 2235 7342 12554 0.7 4.OOE-66 X8921 1.1 NT H.sapiens DNA for endogenous retroviral like element 2425 7524 1.3 4.OOE-66 AJ223364.1 NT Homo sapiens germ-line DNA upstream of Jkappa locus 1408 6535 11707 8.9 3.OOE-66 4502098 NT Homo saplens solute carrier family 25 (mitochonddal carrier; adenine nudeofide translocator), member 5 (SLC25A5), nuclear gene encoding mitochonddal protein, mRNA 6535 11708 8.9 3.OOE-66 4502098 NT Horno saplens solute carder family 25 (niltochondfial carder; adenine nudeotide translocator), member 5 (SLC25A5), nuclear gene encoding mitochonddal protein, mRNA cl, Page 119 of 196 Table 4

0 yz27g112xl Soares multiple sclerosis 2NbHMSP Homo saplens cDNA done IMAGE:284326 Tsimilar to SW:H2,Bl-TIGCA P35068 HISTONE 1-1213.111-1213.2. [2) PIR:1356612; 1939 7056 12249 0.8 3.OOE-66 N55323.11 EST HUMAN yz27g12xl Soares_multiple - scieros!s_2NbHMSP Homo sapiens cDNA clone IMAGE:284326 5'slmilar to SW:H2Bl-TIGCA P35068 HISTONE 1-1213.1111-1213.2.12] PIR:1356612; 1939 7056 12250 0.8 3.OOE-66 N55323.1 EST-HUMAN yz27912.rl Soares_mulfiple-sderosis-2NbHMSP Homo saplens ciDNA clone IMAGE:284326 5'similar to SW:H2Bl-TIGCA P35068 HISTONE 1-1213.111-12B.2.12] PIR:1356612; 1939 7056 12251 0.8 3.OOE-66 N55323.1 EST HUMAN 3079 8230 13329 5.5 3.00IE-66 7662223 NT Homo saplens KIAA0649 gene product (K1AA0649), mRNA 5255. 10369 1.0 2.OOE-66 7657334 NT Homo saRiens Misshapen/NIK-related kinase (MINK), mRNA 5255 10370 1.0 2.OOE-66 7657334 NT Homo sapiens Misshapen/NIK-related kinase (MINK), mRNA Horno saplens origin recognition complex, subunit 5 (yeast homolog)4ike (ORC51---)mRNA, 416 5205 10306 0.9 2.OOE-66 4505524 NT and translated products Homo sapiens origin recognition complex, subunit 5 (yeast homolog)4ike (ORC51---)mRNA, 416 5205 10307 0.9 2.00IE-66 4505524 NT and translated products 1784 6904 12092 0.7 2.00IE-66 AL163301.2 NT Homo sapiens chromosome 21 segment HS21 Cl 01 2944 8095 13201 1.0 2.OOE-66 X65859.1 NT H.sapiens pseudogene for the low affinity IL-8 receptor 3975 9107 14189 0.9 2.OOE-66 AF108389.1 NT Horno saplens sodium/caidum exchanger lsoform NaCa3 (NCX1) mRNA, complete cds 4556 9674 14765 12.7 2.00IE-66 AJ 133267,2 NT Homo sapiens HLA-B gene for human leucocyte antigen B 4556 9674 14766 12,7 2.00IE-66 AJ133267.2 NT Homo sapiens HLA-B gene for human leucocyte antigen B EST01750 Subtracted Hippocampus, Stratagene (cat. #936205) Homo sapiens cDNA clone 4829 9937 0.8 8.OOE-67 M78158.1 EST HUMAN HHCPN31 similar to Ll repetitive element au75d02.xl Schnelder fetal brain 00004 Homo sapiens cDNA done IMAGE:2782083 3' similar to gb:M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 376 5582 10716 2.1 7.OOE-67 AW162232.1 EST-HUMAN PRECURSOR(HUMAN); EST96812 Testis 1 Homo sapiens cDNA Wend similar to similar to C. elegans hypothetical 1360 6488 11654 1.0 7.OOE-67 AA38M16.1 EST HUMAN protein, cosmid ZK353 zh56b05.rl Soares fetal - liver spleerLl NFLS-SI Honno sapiens cDNA clone 1535 6661 11828 1.0 7.OOE-67 W85947.1 EST-HUMAN IMAGE:416049 5' zh56b05xl Soares - fetaUiver-spleen-l NFILS-SI1 Horno sapiens c[DNA done 1535 6661 11829 1.0 7.OOE-67 W85947.1 EST HUMAN IMAGE:416049 5 1988 7103 12305 1.1 7.00IE-67 7657243 NT Homo sapiens Inositol 1,3,4- triphosphate 516 kinase (ITPIKII), mRNA 1988 7103 12306 1.1 7.0012-67 7657243 NT Homo saplens Inositol 1,3,4- tdphosphate 516 kinase (ITPK1), mRNA au75d02.xl Schneider fetal brain 00004 Homo sapiens cIDNA clone IMAGE:2782083 3' similar to gb:M37104 ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL 2779 5582 10716 2.4 7.0012-67 AW162232,1 EST_HUMAN PRECURSOR(HUMAN); 547 5710 10829 1.3 6.OOE-67 X68968.1 NT sapiens mRNA for acetyl-CoA carboxylase 778 5930 11079 0.5 6.OOE-67 Z17227,1 NT Homo sapiens mRNA for transmebrane receptor protein 1252 6381 11540 0.9 6.OOE-67 Y14320.1 NT Horno sapiens PMP69 gene, exons 3,4,5,6 & 7 3- 8280 13388 1.2 6.OOE-67 4506434 NT Homo saffiens retinoblastoma 1 (Including osteosarcoma) (R131) mIRNA Page 120 of 196 Table 4

3391 8533 13638 1.2 6.OOE-67 4507332 NT Homo sapiens Synapsin Ill (SYN3) mRNA, and translated products 3391 8533 13639 1.2 6.OOE-67 4507332 NT Homo sapiens Synapsin Ill (SYN3) mRNA, and translated products 4035 9163 14246 1.3 6.OOE-67 AL163201.2 NT Homo sapiens chromosome 21 segment HS21C001 4035 9163 14247 1.3 6.OOE-67 AL1 63201.2 NT Homo sapiens chromosome 21 segment HS21C001 4607 9721 14815 3.4 6.OOE-67 7657020 NT Homosaple DKFZp43413211 protein (DKFZP434P211), rnRNA 4607 9721 14816 3.4 6.OOE-67 7657020 NT Homo sapiens DKFZp434P21111 protein (DKFZ13434P211 1), mRNA 5125 9988 2,1 6.OOE-67 4507848 NT Homosapiensubiquignspecifi proteasel3(isopepfidaseT-3)(USP13)mRNA 3184 8333 13445 2.5 5.OOE-67 AF009660.1 NT Homo sapiens T cell receptor beta locus, TCRBV7S3A2 to TCRBV12S2 region 1306 6435 11601 0.6 4.0 OE-67 R9081 9,1 EST-HUMAN ynO2d 1 1xl Scares adult brain N2b41-11355Y Homo sapiens cDNA clone 1 MAGE: 167253 5, 2782 5775 10897 0,9 3.OOE-67 AA333768.1 EST HUMAN EST37903 Embryo, 9 week Homo sapiens cDNA Vend 3407 8549 13657 1.1 3.OOE-67 BE064410.1 EST_HUMAN RC4.13T0311-141 199-01 1-h06 BT0311 Homo sapiens cDNA 4596 9710 14802 3.1 3.OOE-67 AW869159.1 EST_HUMAN MR3-SNO066M0500-008-f01 SNO066 Homo sapiens cDNA 5374 10499 0.7 2.OOE-67 BE348354.1 EST-HUMAN hwl 6909.xl INIC1-WAPLu24 Homo sapiens cIDNA done IMAGE:3183136 Ysimilar to WPY231-11 1.9 CE09617; 827 5976 11136 2.0 2.OOE-67 AW816405.1 EST HUMAN QV4-ST0234-181199-037- f05 ST0234 Homo sapiens cDNA 1089 6227 0.6 2.OOE-67 AF167460.1 NT Homo sapiens double stranded RNA activated protein kinase (PKR) gene, exons 2a, 2, 3, and 4 1841 6959 12146 0.5 2.OOE-67 BE303037.1 EST-HUMAN ba72g05.yll N1H - MGQ_20 Homo sapiens cIDNA done IMAGE:2905976 Ssimilar to TR:094892 094892 KIAA0798 PROTEIN.; 1841 6959 12147 0.5 2.OOE-67 BE303037.1 EST HUMAN ba72905.yl INIM-MGQ_20 Homo sapiens cIDNA clone IMAGE:2905976 Tsimilar to TR:094892 094892 KIAA0798 PROTEIN.:

2381 7483 12685 1.2 2.OOE-67 4758795 NT Homosa s developmentally regulated GTP-binding protein 1 (DRG1), mRNA 3422 8564 13676 3.9 2.OOE-67 AA625755.1 EST HUMAN zu91g01.sl Soares testl HT Homo sapiens cDNA done IMAGE:745392 3' 3921 9055 14142 2.3 2.OOE-67 AL163300.2 NT Homo saple s chromosome 21 segment HS21 Cl 00 4716 9828 1.1 2.0OET7 AA418535.1 EST HUMAN zv92g09.rl Soares_WHIVIPu S1 Homo saplens cDNA done IMAGE:767296 5, 253 5441 10568 1.5 1.OOE-67 4502166 NT Homo sapiens arnylold beta (A4) precursor protein (protease nexin-li. Alzhelmer disease) (APP), mRNA 3794 8931 14024 5.0 8.OOE-68 AA20M6.1 EST-HUMAN zq82h11 0xl Stratagene hNT neuron (#937233) Homo saplens cDNA clone IMAGE:648163 V similar to SW:SAV SULAC Q07590 SAV PROTEIN., 3794 8931 14025 5.0 8.OOE-68 AA209456.1 EST HUMAN zq82h10xl Stratagene hNT neuron (#937233) Homo sapiens cIDNA clone IMAGE:648163 V similar to SW:SAV_SULAC 007590 SAV PROTEIN.; 1849 6967 0.8 6.OOE-68 AWS03842.1 EST HUMAN Ul-HF-BNO-alb-c-07-0-Ui.rl N11-1_1VIGC 50 Homo sapiens cDNA clone IMAGE:3078924 S' 56 5265 10386 0.9 5.OOE-68 AA304324.1 EST HUMAN EST17103 Aorta endothelial cells, TNF alpha-treated Homo sapi ns cIDNA Vend 56 5265 10387 0.9 5.OOE-68 AA304324.1 EST_HUMAN EST17103 Aorta endothelial cells, TNF alpha-treated Homo sapiens cIDNA Tend 57 5265 10386 1.0 5.OOE-68 AA304324.1 EST-HUMAN EST17103 Aorta endothelial cells, TNF alpha-treated mc, sapiens cDNA 5' end 57 5265 10387 1.0 5.OOE-68 AA304324.1 EST HUMAN EST1 7103 Aorta endotheliall cells, TNF alpha-treated Homo saelons cDNA 5' end 78 7864 11087 0.9 5.OOE-68 AF231919.1 NT Homo saplens chromosome 21 unknown mRNA 7864 11088 0.9 5.OOE-68 AF231919.1 NT Homo saplens chromosome 21 unknown mRNA "6 802 11104 1.3 5.OOE-68 AF231919.1 NT Homo sapiens chromosome 21 unknown mRNA J785 t5953 11105 1.3 5.OOE-68 AF231919.1 NT - 5953 Homo sapiens chromosome 21 unknown mRNA 595 Page 121 of 196 Table 4

0 3108 8258 13362 2.6 5.OOE-58 AB037852.1 NT Homo sapiens mRNA for KIAA1431 protein, partial ods 4900 10007 17.2 4.OOE-68 P04406 SWISSPROT GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER 3611 8750 13844 5.6 3.OOE-68 AF236082.1 NT Mus musculus G-protein coupled receptor 9PR73 (Gpr73) mRNA, complete cds 2832 7983 12.3 2.OOE-68 D00522.1 NT Cricetulus longicaudatus mRNA for EF- 1 alpha. complete cds 4583 9699 14793 1.7 2.OOE-68 A8008681.1 NT Homo sapiens gene for activin receptor type JIB, complete cds 72 5278 10405 1.3 1.OOE-68 4505222 NT Homo saplens meningloma (disrupted In balanced translocation) 1 (MN1), MRNA 293 5479 10610 3.2 1.0OE-68 AW816405.1 EST-HUMAN QV4-ST0234-181199-037- f05 ST0234 Horno sapiens cDNA 2205 7314 12526 1.3 1.OOE-68 AF1 19860.1 NT Homo sapiens PR02014 mRNA, complete cds 2205 7314 12527 1.3 1.OOE-68 AF1 19860.1 NT Homo saplens PR02014 mRNA, complete cds 2728 7812.13018 1.0 1.OOE-68 AW451832.1 EST-HUMAN UI -H-B13-alk-f-01-0-Ul. sl NCICGAP-Sub5 Homo sapiens c[DNA done IMAGE:2737272 3, 3928 9062 14149 1.0 1.OOE-68 BE296032.1 EST-HUMAN 601177002F1 NIH MGCL17 Horno saplens cDNA done IMAGE:3532344 5' 4954 10059 15130 1.0 1.OOE-68 AA897343.1 EST HUMAN a147g12.si Soares NFL T GBC S1 Homo.sapiens cIDNA done IMAGE:1460518 3, 19 5228 10329 0.6 9.OOE-69 5031976 NT Homo saRiens pre-B-cell colonyenhancing factor (PBEF) mRNA 19 5228 10330 0,6 9.OOE-69 5031976 NT Homo sap 1 lens pre-B-cell colony- enhancing factor (PBEF) mRNA 1011 6151 11304 0.6 9.OOEM 5031980 NT Horno sapiens 26S proteasome- associated padl homolog (P0H1) mRNA 1011 6151 11305 0.6 9.OOE-69 5031980 NT Homo sapiens 26S proteasome- associated padl homolog (P0H1) mRNA 4037 9165 14250 0.8 9.00IE-69 4757867 NT Homo saplens v-raf mudne sarcoma viral oncogene homolog B1 (BRAF) mRNA 3340 R8-4 1.2 8.OOE-69 AJ237744.1 NT Homo sapiens RIBI1R gene (partial), exon 12 509 5673 0.6 4.OOE-69 A1873630.1 EST HUMAN wm26hl 1.xl NCI CGAP -Ut4 Homo saplens cDNA done IMAGE:2437125 3' 380 5586 10719 1.0 3.00IE-69 BE258012.1 EST HUMAN 601110371 F1 NIH_MGQ_1 6 Horno sapiens cDNA done IMAGE:3351352 5' 596 5754 10872 0.6 3.OOE-69 AF221712.1 NT Homo sapiens Smad- and Olf- interacting zinc finger protein mRNA, partial cds 1536 6662 1.4 3.OOE-69 T80514.1 EST HUMAN yd08a02xl Soares infant brain 1 NIB Homo saplens cDNA done IMAGE:24880 5' similar to SP:A48836 A48836 SPEGF 111=EGIZ REPEAT-CONTAI4NG FIBROPELLIN-LIKE PROTEIN SEA URCHIN; 2325 7428 0.9 3.OOE-69 5729910 NT Horno sapiens lymphatic vessel endothelial hyaiuronan receptor 1 (LYVE-1) mRNA 3990 9122 14206 5.9 3.OOE-69 A1765888.1 EST HUMAN wh66g08.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:2385758 3, 124 5566 10702 1.0 2.OOE-69 AF160252.1 NT Homo sapiens KIAA0553 protein gene, complete cds; and alphalib protein gene, partial cds 124 5566 10703 1.0 2.OOE-69 AF160252.1 NT Homo sapiens KIAA0553 protein gene. complete cds; and alphallb protein gene, partial cds 398 5566 10702 1.7 2.0011-69 AF160252.1 NT Homo sapiens KIAA0553 protein gene, complete cds; and alphallb protein gene, partial cds 398 5566 10703 1.7 2.OOE-69 AF160252.1 NT Homo sapiens KIAA0553 protein gene, complete cds; and alphallb protein gene, partial cds 1842 6960 12148 1.2 2.OOE-69 BE257857.1 EST-HUMAN 601109444F1 NIH MGC-16 Homo sapiens cDNA done IMAGE:3350074 S' 2813 7964 2.7 2.OOE-69 AA431157.1 EST-HUMAN zw71a02.rl Soares testis NHT Homo saplens ciDNA done IMAGE:781682 5, 1675 6799 11977 0.9 11.0OE-69 AF053768.1 NT Rattus norvegicus brain specific cortactin-binding protein CBP90 mRNA, partial cds 2284 7932 12597 0.6 8.OOE-70 AA230303.1 EST-HUMAN nc13d12xl NCI CGAP PrI Homo sapiens cDNA clone IMAGE:1008023 4277 9402 14486 2.2 8.OOE-70 1.77566.1 NT Horno saplens DGS.1 mRNA, Yend 6892 0.7 7.OOE-70 A1497807.1 EST_HUMAN tm89f01.xl NCI CGAP Pm25 Homo saplens cDNA done IMAGE:2165305 3' Page 122 of 196 Table 4 -P 1771 188 201 4132 853 2090 2461 2505 2505 1571 1571 674 674 689 1004 1167 1167 1387 1708 2272 2344 2344 3963 3347 3469 1743 3717 1818 1818 2576 2163 4030 6892 12080 0.7 7.OOE-70 A1497807.1 EST-HUMAN tm89f011.xl NCI CGAP 13m25 Homo sapiens cDNA clone IMAGE:2165305 3' 7006 12192 0.6 7.OOE-70 AA282955.1 EST-HUMAN ztl 5h04.rl NCI-CGAP GC131 Horno, saplens cIDNA done IMAGE:713239 V 7131 1.3 7.OOE-70 5031668 NT Homo sapiens tumor suppressor deleted In oral cancer-related 1 (DOC-1 R) mRNA 9258 14339 3,9 7.OOE-70 4757723 NT Homo sapiens adenate cyclase 3 (ADCY3) mRNA 6002 11162 0.7 6.0012-70 4502166 NT Homo sapiens amAold beta (A4) precursor protein (protease ne)dn-li, Alzheimer disease) (APP), mRNA 7201 12406 1,4 6.OOE-70 M30938.1 NT Human Ku p701p80) subunit mRNA, complete ods 7560 12764 1.0 6.0OE-70 8923899 NT Horno sapiens CMP-N-acetyineuraminic acid synthase (LQC55907), mRNA 7M 12800 0,7 5.OOE-70 7662307 NT Homo sapiens WAA0792 gene product (K1AA0792), mRNA 7945 12801 0.7 5.0OE-70 7662307 NT Horno sapiens KIAA0792 gene product (K1AA0792), mRNA 6697 11864 0.9 3.OOE-70 BE071796.1 EST-HUMAN RCO-BT0522-071299-01 1 -al 2 BT0522 Horno sapiens ciDNA 6697 11865 0.9 3.OOE-70 BE071796.1 EST HUMAN RCO-BT0522-071299-01 1 -al 2 BT0522 Horno sapiens cDNA 5830 10958 4.5 2.0OE-70 N42161.1 EST HUMAN yy07a10xl Soares melanocyte 2NbHM Homo sapiens ciDNA done IMAGE:270522 5'similar to SW:1D31-11-RAT P29266 3-HYDROXYISOBUTYRATE DEHYDROGENASE PRECURSOR; 5830 10959 4.5 2.OOE-70 N421611.1 EST-HUMAN yy07a10xl Soares melanocyte 2Nbi-IM Homo saplens cDNA clone IMAGE:270522 Csimilar to SW:1331-11-RAT P29266 3-HYDROXYISOBUTYRATE DEHYDROGENASE PRECURSOR; 5844 10981 0.7 2.OOE-70 A1246899.1 EST - HUMAN qx51h01.xl NCI CGAP Panll Homo sapiens cIDNA done IMAGE:2004913 3 6144 11298 0,6 2.OOE-70 8923669 NT Homo sapiens hypothetical protein FLJ20758 (FLJ20758), mRNA 6501 11456 0.7 2.OOE-70 7661983 NT Homo saplens KIAA0193 gene product (K1AA:0193), mRNA 6301 11457 0.7 2.OOE-70 7661983 NT Homo saplens KIAA0193 gene product (K1AA0193), mRNA 6514 11680 1.0 2.OOE-70 BE46731 1.1 EST_HUMAN hz64c12.xl NCI CGAF_Lu24 Homo sapiens cDNA done IMAGE:3212758 3 6831 12011 0.8 2.OOE-70 AL163202.2 NT Homosa ns chromosome 21 segment HS21C002 7378 1,3 2.OOE-70 AA054010.1 EST HUMAN zf48904.rl Soares retina N2b41-1R Homo sapiens cIDNA done IMAGE:380214 Tsimilar to SW:GAG HT0A P03345 GAG POLYPROTEIN; 7446 12653 2.9 2.OOE-70 AW961822.1 EST-HUMAN EST373895 MAGE resequences, MAGG Homo saplens ciDNA 7446 12654 2.9 2.OOE-70 AW961822.1 EST HUMAN EST373895 MAGE resequences, MAGG Homo sapiens ciDNA 9095 14180 4.0 2.OOE-70 M69181.1 NT Human nonmusde myosin heavy chain-B (MYHIO) mRNA, partial cds 8491 3.0 1.OOE-70 4507476 NT Homo saplens transolutarninase 3 (E polypeptide, proteln-glutamine-gamma glutamtransferase) (TGM3) mRNA 8610 13722 1.0 9.OOE-71 AW936999.1 EST HUMAN CM3-DT0032-151299-055-h12 DT0032 Homo sapiens cDNA 6866 12044 0.6 8.OOE-71 AA594019.1 EST-HUMAN rin28e07.sl NCI CGAP Gasl Homo sapiens cDNA done IMAGE:1085220 3' 8854 13946 0.9 7.OOE-71 BE244676.11 EST-HUMAN TCBAP2E0520 Pedlatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TC13A Homo saplens cONA clone TCBAP0520 6937 12127 1.0 6.OOE-71 7705404 NT Homc saplens hernelinding protein (HEBP), mRNA 6937 12128 1.0 6.OOE-71 7705404 NT Homo sapiens heme-binding protein (HEBP), mRNA 7669 0.8 6.OOE-71 BE266255.1 ESTHUMAN 601191652F1 NIH_MGCJ Homo sapiens cDNA done IMAGE:3535549 5' 7272 12483 1.3 5.OOE-71 AF056322.1 NT - Homo sapiens SP100-HMG nuclear autoanfigen (SP100) mRNA, complete cds 9158 14242 1.4 5.OOE-71 AW816405.1 ESTHUMAN QV4-ST0234-181199-037405 ST0234 Homo saplens cDNA 10239 15314 3.2 5.0OE-711 A1829496.1 ESTHUMAN w118h10.xl NCI CGAP Utl Homo saplens cDNA done IMAGE:2425315 3' Page 123 of 196 Table 4 -A 97 5304 10431 1.1 4.0012-71 4507592 NT Homo sapiens tumor necrosis factor (ligand) superfamily, member 10 (TWSF10) mRNA 347 5528 10655 39.0 4.OOE-71 AF1157626.1 NT Equus caballus glyceraidehyoe- 3-phosphate dehydrogenase mRNA, partial cds 347 5528 10656 39,0 4.OOE-71 AF157626.1 NT Equus caballus glyceraldehyde- 3-phosphate dehydrogenase mRNA, partial cds 2850 8001 13098 0,9 4.OOE-71 7705414 NT Homo sapiens hookl protein (HOOK1), mRNA 2850 8001 13099 0.9 4.0012-71 7705414 NT Homo sapiens hookI protein (HOOK1), mRNA 2857 8008 13107 1.6 4.OOE-71 4505880 NT Homo saplens plasminogen (PLG) mRNA 4330 9453 14541 3.4 4.0OE-711 AF056322.1 NT Homo sapiens SP1 00-HMG nuclear autoandgen (SP1 00) mRNA, complete eds 4913 10020 15099 5.0 4.OOE-71 7657602 NT Homo sapiens putative heme- binding protein (SOUL), mRNA 1210 11500 0.9 2.OOE-71 ALI 63206.2 NT Homo saplens chromosome 21 segment HS21 C006 622 5781 10903 0.7 1.OOE-71 A1077927.1 EST---HUMAN oyl 5e03.sl Soares- senescent-fibroblasts-NbHSF Homo sapiens cIDNA done IMAGE:1665916 X similar to contains LOR1112 LOR1 repetitive element; 926 6073 11231 0,7 1.OOE-71 7706281 NT Homo sapiens neuronal cell death- related protein (LOC51616), mRNA 1084 6222 11373 1,4 1.0012-71 AF205890.1 NT Homo sapiens disabled-2 gene, exons 2 through 15 and complete cds 1317 6446 11612 3.8 1.OOE-71 AF012872.1 NT Homo sapiens phosphafidylinositol 4-kinase 230 (pi41K230) mRNA, complete ods 2036 7149 12353 1.2 1.ooE-71 ABo17007.1 NT Homo sapiens PMS2L16 mRNA, partial cds 2036 7149 12354 1.2 1.OOE-71 A13017007.1 NT Homo sapiens PMS2LI6 mRNA, pardal cds 2661 77W 12948 1.8 1.OOE-71 7657153 NT Homo sapiens hatrylenhancer-of- split related vAth YRM motlf-like (HEYL), mRNA 3457 8598 13711 1.2 1.OOE-71 AF1 19665.1 NT Homo sapiens Inorganicpyrophosphatase mRNA, complete cds 3546 8687 13788 4.7 1.OOE-71 AF246219.1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 3546 8687 13789 4.7 1.0OE-71 AF246219.1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 3597 8736 13830 0.9 11.0OE-71 BE122850.1 EST HUMAN 02 15 Human Epidermal Keratinocyte Subtraction Library- Upregulated Transcripts Homo sapiens cDNA clone 02 15 V similar to Honno sapiens chromosome 19 3597 8736 13831 0.9 1.00IE-71 BE122850.1 EST-HUMAN 02 Human Epidermal Keradnocyte Subtraction Library- Upregulated Transcripts Homo sapiens cDNA clone 02 15 Csimilar to Horno saplens chromosome 19 3681 8820 13913 1.9 1.OOE-71 AF218904.1 NT Homo sapiens attractin precursor (ATRN) gene. exon 19 4370 9492 14582 1.9 1.OOE-71 D28476.1 NT Human mRNA for KIAA0045 gene, complete cds 401 5569 10705 1.2 9.ODE-72 A1857635.1 EST-HUMAN wk95903.xl NCl-WAP-Lulg Homo sapiens cDNA done IMAGE:2423188 3'sImilar to TR:086705 086705 HYPOTHETICAL 38.6 KID PROTEIN,;contains Alu repetitive element; 401 5569 10706 1,2 9.ODE-72 A1857635.1 EST HUMAN M95g03.xl NCI-CGAP-Lul9 Homo sapiens cIDNA done IMAGE:2423188 Ysimilar to TR:086705 086705 HYPOTHETICAL 38.6 KID PROTEIN.;contains Alu repetitive element; 4023 9151 14232 5.2 7.OOE-72 4501866 NT Homo sapiens aconitase 2. mitochondrial (AC02), nuclear gene encoding mitocondriall protein, mRNA 4023 9151 14233 5.2 7.OOE-72 4501866 NT Homo saplens aconitase 2, mitochondrial (AC02), nuclear gene encoding mitocondrial protein, mRNA 4023 9151 14234 5.2 7.OOE-72 4501866 NT Homo sapiens aconitase 2, mitochondrial (AC02), nuclear gene encoding mitocondrial protein, mRNA, 1122 6259 0.9 5.OOE-72 L1 1645.1 NT Homo saplens alpha-tubulin mRNA, complete cds 5404 10528 1.1 4.OOE-72 8922990 NT Homo sapiens hypothetical protein FU1 1316 (FU1 1316), mRNA Page 124 of 196 Tabie 4 885 6033 4.9 3.OOE-72 AA723823.1 EST-HUMAN ah63a06.si Soares testis NHT Homo sapiens cIDNA done 1310290 3, 1139 6275 11426 2,1 3.OOE-72 U16306.1 NT Human chondroldn subte proteoglycan versican VO splice-varlant precursor pepfide mRNA, complete ods 1139 6275 11427 2.1 3.OOE-72 U16306.1 NT Human chondroifin sulfate proteoglycan versican VO splice-vadant precursor peptide mRNA, complete cds 3037 8189 13291 10.5 3.OOE-72 AJ229043.1 NT Homo sapiens 959 kb config between AML1 and CBRI on chromosome 21 q22, segment 313 3241 8388 13495 2.6 3.OOE-72 8923548 NT Homo sapi ns hypothetical protein FLJ20585 (FU20585), mRNA 3750 8888 13977 2.5 3.OOE-72 S77589.1 NT TCR V delta 2-C alpha =T-cell receptor delta and C alpha fusion gene (alternatively spliced, splice Junction} [human, precursor B-cell line REH, mRNA Partial, 211 ntl 4835 9943 15032 0,9 3.OOE-72 A1654337.1 ESTHUMAN w1J31a08.xl NCI CGAP GC6 Homo saplens cIDNA done IMAGE:2307254 3 2030 7143 12344 0.9 1.OOE-72 AA846225.1 EST-HUMAN M83d02.sl Soares_parathyroiO_tumoLNbHPA Homo sapiens cDNA done IMAGE: 1387395 3' 1443 6570 11745 1.2 9.OOE-73 AW374968.1 EST HUMAN MRO-CT0063-071099-002- h11 1 CTO063, Homo saplens cDNA 5102 10198 15278 1.0 9.0OE-73 AW867850.1 EST-HUMAN MRO-SN0040-050500-002- e01 SNO040 Homo saplens cDNA 1022 6161 11314 1.0 8.OOE-73 AW071755.1 EST HUMAN ws55c06.xl NCI CGAP 13m25 Homo sapiens cDNA clone IMAGE:2501098 Ysimilar to TR:Q59050 Q5j050 HYPOTHETICAL PROTEIN MJ1656.; 1399 6526 11695 1.1 8.OOE-73'A1024877.1 EST-HUMAN ov39h08.xl Soares_tesds_NI-IT Homo sapiens cDNA clone IMAGE: 1639743 3, 1118 6255 11407 0.8 7.OOE-73 8923290 NT Homo saplens hypothetical protein FLJ20309 (FU20309), mRNA 3261 8408 13515 1.1 7.OOE-73 AL-163206.2 NT Homo saplens chromosome 21 segment HS21C006 4863 9970 1.6 7.OOE-73 AL163282.2 NT Homo sapiens chromosome 21 segment HS21 C082 152 5348 0.8 6.OOE-73 AL163218.2 NT Homo sapiens chromosome 21 segment HS21C018 1247 6376 11534 1.4 3.OOE-73 F00548.1 EST-HUMAN HSBIOE082 STRATAGENE. Human skeletal muscle cDNA library, cat. #936215. Homo sapiens cIDNA done 10E08 1247 6376 11535 1.4 3.OOE-73 F00548.1 EST HUMAN HSB1OE082 STRATAGENE Human skeletal muscle cDNA library. cat. #936215. Homo sapiens cIDNA clone 10E08 833 5982 11142 0.6 2.OOE-73 AF139897.1 NT Homo sapiens BASS1 (BASSI) mRNA, partial ods 1902 7020 -0.5 2.OOE-73 AW898081.1 ES7_HUMAN RC3-NNO066-270400-01 1-c04 NNO066 Homo saplens cIDNA 2251 7357 1.1 2.OOE-73 U01317.1 NT Human beta globin region on chromosome 11 3144 8293 13401 3.5 2.OOE-73 4502582 NT Homo sapiens caspase 8, apoptosts- related cystelne protease. (CASP8) mRNA 3503 8645 13753 1.0 2.OOE-73 7669539 NT Homo sapiens Parkinson disease (autosomal recessive, juvenile) 2, parkin (PARK2), transcript variant 3, mRNA 3503 8645 13754 1.0 2.OOE-73 7669539 NT Homo sapiens Parkinson disease (autosomal recessive, juvenile) 2, parkin (PARK2), transcript variant 3, mRNA 2434 7533 12739 0.9 1.0OE-73 AF198349.1 NT s Dach2 protein (Dach2) mRNA, complete cds 2073 7185 12388 1.0 9.OOE-74 AW054828.1 EST_HUMAN ws60d04xl NCLCGAP - Bm25 Homo sapiens cDNA done IMAGE:2501575 3'similar to TR:Q13541 013541 4E.BINDING PROTEIN 1.

723 5877 11017 1.4 8.OOE-74 4557426 NT Homo sapiens CD39-like 4 (CID39L4) mRNA 1906 7023 12211 1.1 7.OOE-74 A.1001689.1 NT Homo saplens NKG2D gene, exon 10 32861 1.0 7.OOE-74 AL163246.2 NT Horno sapiens chromosome 21 segment H$21C046 0 Page 125 of 196 1 Table 4 j C 1106 6244 11397 0.9 6.OOE-74 AF109907.1 NT Homo sapiens S164 gene. partial cds; PS1 and hypothetical protein genes, complete cds; and S171 gene, partial cds 1609 6736 11905 1.0 6.OOE-74 AW263177.1 EST-HUMAN xn78g07.xl Soares NFL T GBC S1 Homo sapiens cDNA done IMAGE:2700636 3' 2268 7374 12582 3.6 6.OOE-74 BE388260.1 EST-HUMAN 601283521 F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE:3605453 6 2268 7374 12583 3.6 6.OOE-74 BE388260.1 EST-HUMAN 601283521F1 NIH MGC 44 Homo sapiens cDNA done IMAGE:3605453 5' 2834 7985 13085 1.4 6.OOE-74 AW014039.1 EST HUMAN U]-H-BIO-aah-h-03-0-Ul. sl NCLWAP-Subl Homo sapiens cDNA done IMAGE:2709365 3' 2834 7985 13086 1.4 6.OOE-74 AW014039.1 EST-HUMAN Ul-H-BIO-aah-h-03-0-Ul. sl NCLWAP-Subl Homo sapiens cDNA done IMAGE:2709365 3' 3652 8791 13884 2.6 6.OOE-74 BE048846.1 EST HUMAN hr54el 1.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:3132332 X 3652 8791 13885 2.6 6.OOE-74 BE048846.1 EST-HUMAN hr54el 1.xl NCI CGAP Kidl 1 Homo sapiens cDNA done IMAGE:3132332 3, 4826 9934 15024 1.2 6.OOE-74 H09912.1 ESTHUMAN ym05g03xl Soares infani brain 1 NIB Homo sapiens cDNA done IMAGE:46895 5, 887 6035 11193 0.9 5.OOE-74 AW020986.1 EST-HUMAN di17c09.yl Morton Fetal Cochlea Homo sapiens cDNA done IMAGE:2483704 5' 2669 7756 5.2 5.OOE-74 AW362756.1 EST-HUMAN PMO-CTO289-271 099-001 -h07 CTO289 Homo sapiens cDNA 278 5465 10595 0.6 4.OOE-74 D87675.1 NT Homo sapiens DNA for amytoid precursor protein, complete cds 834 5983 11143 1.7 4.0012-74 AB028942.1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 1919 7036 12224 0.9.4.OOE-74 A13026898.1 NT Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 112 (DLEC1, ORCTL3, ORCTL4 genes, complete cds) 1919 7036 12225 0.9 4.OOE-74 A13026898.1 NT Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 112 (DLEC1, ORCTI-3, ORCTI.4 genes, complete cds) 2027 7140 12340 1.6 4.OOE-74 4506192 NT Homo sapiens proteasome (prosome, macropain) subunit, beta type, 1 (PSIVII31) mRNA, 2027 7140 12341 1.6 4.OOE-74 4506192 NT Homo saplens proteasome (prosome, macropain) subunit, beta type, 1 (PSMB1) mRNA 2085 7197 12402 1.2 4.OOE-74 AB032994.1 NT Homo sapiens mRNA for K1AA1 168 protein, partial cds 2377 7479 12681 1.0 4.OOE-74 AJ006976.1 NT Homo sapiens PLP gene 3052 8203 13304 4.4 4.OOE-74 AJ006976.1 NT Homo sapiens PLP gene 3483 8624 13736 0.9 4.OOE-74 AL163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 3973 9105 14187 1.3 4.OOE-74 AL163247.2 NT Homo sapiens chromosome 21 segment HS21C047 4457 9577 14674 1.6 4.OOE-74 7662183 NT Homo saplens KIAA0569 gene product (K1AA0569), mRNA 4512 9631 14724 0.8 4.OOE-74 Z17227.1 NT Homo sapiens mRNA for transmebrane receptor protein 5006 10108 15176 3.8 4.OOE-74 4504326 NT Homo sapiens hydroxyacyl- Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolaselonoyi-Coenzyme A hydratase (trifunctional protein), beta subunit (HADHB) mRNA 5006 10108 15177 3.8 4.OOE-74 4504326 NT Homo sapiens hydroxyacyl- Coenzyme A dehydrogenase/3-ketoacyl-Ceenzyme A thiolaselenoyi-Coenzyme A hydratase (trifunctlonal protein), beta subuffit (HADH13) mRNA 2849 8000 13096 1.1 3.OOE-74 AA234789.1 EST-HUMAN zr78b1O.rl Soares- NhHMPv_Sl Homo sapiens cDNA done IMAGE:669499 5'similar to gb:X04106 cdsl CALCIUM-DEPENDENT PROTEASE, SMALL (HUMAN); 2849 8000 13097 1.1 3.OOE-74 AA234789.1 EST HUMAN zr78b1O.rl Soares- NhHIVIPu-SI Homo sapiens cDNA done IMAGE:669499 5'similar to gb:X0410Q._cdsl CALCIUM-DEPENDENT PROTEASE. SMALL (HUMAN); 32 8351 2.2 3.OOE-74 A1796960.1 EST HUMAN we25c07.xl NCl-WAP-Lu24 Homo sapiens eDNA done IMAGE:2342124 3'similar to TR:Q13537 013537 SIMILAR TO POGO ELEMENT.; U Page 126 of 196 0 Table 4 f(_4 3703 8000 13096 4.8 3.OOE-74 AA234789.1 EST-HUMAN zr78b1O.rl Soares_Nhi- IMPu-S1 Horno sapiens cDNA done IMAGE:669499 V similar to gb:X04106 cdsl CALCIUM-DEPENDENT PROTEASE, SMALL (HUMAN); 3703 8000 13097 4.8 3.OOE-74 AA234789.1 EST HUMAN zr78b1O.rl Soares-NhiIMPq_S1 Homo saplens cDNA done IMAGE:669499 5'similar to gb:X04106 cdsl CALCIUM-DEPENDENT PROTEASE, SMALL (HUMAN); 942 60U9 11246 126.2 2.OOE-74 7669491 NT Homo sapiens glyceraidehyde-3- phosphate dehydrogenase (GAPD), mRNA 942 6089 11247 126.2 2.OOE-74 7669491 NT Homo sapiens glyceraidehyge-3- phosphate dehydrogenase (GAPD), mRNA 1158 6293 11448 1.0 2.OOE-74 AF020092.1 NT Human endogenous retrovirus HEW-K-T47D 1224 6354 11518 1.2 2.OOE-74 A1950528.1 EST-HUMAN wx5le07.xl NCI_Q_Lu28 Homo sapiens cDNA done IMAGE:2547204 Ysimilar to 1577 6703 11872 1.1 2.0011-74 4885198 NT SW:GG95 HUMAN Q08379 GOLGIN-95.; contains eieTgnt MER22 repetitive element Homo sapiens epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) (EGF) mRNA 1577 6703 11873 1.1 2.OOE-74 4885198 NT Homo sapiens epidermal dro-wfh- factor receptor (avian erythroblastic leukernia viral (v-erb-b) oncogene homolog) (EGFR) mRNA 2558 7652 12855 1.1 2.OOE-74 A1557280.1 EST HUMAN PT2.1 15 G1 1.r tumor2 Homo saplens cDRA 3' 4937 10043 15114 2.0 2.OOE-74 AL355092.1 NT Novel human gene mapping to chomosome 22 4937 10043 15115 2.0 2.OOE-74 AL355092.1 NT Novel human gene mapping to chomosome 22 4942 10048 15120 1.9 2.OOE-74 J02963.1 NT Human platelet glycopro in lib mRNA, Y end 47 5257 10373 1.0 1.0OE-74 7657334 NT Homo sap ens Misshapen/NIK-related kinase (MINK), mRNA 334 5515 10640 1.2 1.0OE-74 AW816405.1 EST HUMAN QV4-ST0234-181199-037- f05 ST0234 Homo sapiens cIDNA 491 5656 10779 1.2 1.OOE-74 8922829 NT Homo sapiens hypothetical protein FLJ 11026 (FLA 1026), mRNA 497 5661 10784 0.9 11.0OE-74 X02344.1 NT Homo sapiens beta 2 gene 586 5746 10863 1.4 1.OOE-74 4508020 NT Horno sapiens dric fin r protein 259 (ZNF259) mRNA 983 6128 11283 0.7 1.OOE-74 AL163246.2 NT Homo, sapiens chromosome 21 segment HS21C046 2179 758 12500 2.2 1.OOE-74 ABOO2059.1 NT Homo sapiens DNA for Human P2XM, . complete cds 3100 8250 13354 6.0 1.OOE-74 4758697 NT Homo sapiens mannosidase, alpha, class 2A, member 1 ( N2A1), mRNA 3887 9023 14116 4.6 1.OOE-74 AL163268.2 NT Homo sapiens chromosome 21 segment HS21 C068 3974 9106 14188 0.9 11.0OE-74 BE083080.11 EST HUMAN RC2-13T0642-270300- 019406 BT0642 Homo sapiens cDNA 4170 9296 14377 0.9 1.OOE-74 BE467769.1 EST-HUMAN h273h08.xl NCICGAP_Lu24 Homo saplens cDNA done IMAGE:3213663 Ysimilar to WP:130511.12 CE17351; 5112 10208 15286 1.2 1.OOE-74 D83327.1 NT Homo sapiens WRRI mRNA, partial cds 2607 7697 1.5 8.OOE-75 AF176228.1 NT Homo saplens DNA cytosine-5 methyltransferase 313 (DNIVIT313) mRNA, complete cds 2273 7379 12587 0.9 6.OOE-75 A1817415.1 EST HUMAN wk38a0B.xl NCI_PGAF_Pr22 Horno saplens cDNA done IMAGE:2417654 Ysimilar to gb:M14123_cds4 RETROVIRUS-RELATED POL POLYPROTEIN (HUMAN); 5128 10223 0.9 6.0012-75 AA789285.1 EST-HUMAN aj28c06.sl Soarek_estiR_NHT Horno saplens cDNA done 1391626 Ysimilar to TR:Q115377 Q15377 Y-CHROMOSOME RNA RECOGNITION MOTIF PROTEIN; 107 5310 10435 1,1 4.OOE-75 BE081333.1 EST HUMAN QV1-BT0632-210200-079- e02 BT0632 Homo saplens cDRA 451 5617 1.2 4.OOE-75 N36757.1 EST-HUMAN yx90h08xl Scares melan;5; 2Nbi- IM Homo sapiens cDNA clone IMAGE:269055 5 1728 6891 12030 0.5 4.OOE-75 AW897230.1 EST-HUMAN CMO-NNO057-150400-335- al 1 NNO057 Homo sapiens cDNA 2818 7969 13072 4.7 4.OOE-75 BE409464.1 EST-HUMAN 601303866F1 NiH MGC 21 Horno saplens cDNA done IMAGE:3638344 5' 986 6131 11286 1.0 3.OOE-75 AF157623.1 NT Homo sapiens HTRA sedne protease (PRSS1 1) gene. com lete cds 6131 11286 0,8 3.OOE.75 AF157623.1 NT Homo sapiens HTRA sedne protease (PRSSI 1) gene, complete cds Page 127 of 196 Table 4

1795 6915 12101 0.7 3.OOE-75 AB011153.1 NT Horno sapiens mRNA for KIAA0581 protein, partial cds 2065 7177 12384 1.1 3.OOE-75 4507334 NT Horno saplens synaptojanin 1 (SYNA), mRNA 2373 7475 12676 1.3 3.OOE-75 4759153 NT Homo saplens synaptosomal- associated protein, 29k13 (SNAP29) mRNA zk53c07.sl Soares_pregnant-uterus_NbHPU Homo sapiens cDNA done IMAGE:486540 3' similar to qb:X65857_0sl OLFACTORY RECEPTOR-LIKE PROTEIN HGMP07E (HUMAN); 2402 7501 12708 1.0 3.OOE-75 AA042813.1 EST HUMAN 2988 8140 13243 1.2 3.OOE-75 AL1 63201.2 NT Homo sapiens chromosome 21 segment HS21 C001 3152 8301 13409 1.3 3.OOE-75 AB011153.1 NT Homo sapiens mRNA for KIAA0581 protein, partial eds 3306 8451 13562 1.0 3.OOE-75 M72393.1 NT Human caidum-dependent phospholipid-binding protein (PLA2) mRNA, complete ods 3306 8451 13563 1.0 3.OOE-75 M72393.1 NT Human calcium-dependent phospholipid-binding protein (PLA2) mRNA, complete cds 4077 9204 14291 1.4 3.OOE-75 D87675.1 NT Homo sapiens DNA for arnylold precursor protein, complete.cds 4336 9459 14547 1.0 3.OOE-75 7662421 NT Homo saplens KIAA0971 protein (K1AA0971), mRNA Ysimilar to x960d02.xl NCI-CGAP_Ut4 Homo sapiens ODNA done iMAGE:2632707 2255 7361 12569 1.8 1.OOE-75 AW168135.1 EST HUMAN contains PTR7.d PTR7 repetitive element; 2916 8067 13176 3,2 1.0OE-75 X52221.1 NT H.sapiens ERCC2 g ne, exons 1 & 2 (partial) wb30b10.xl NCl-WAP_GC6 Horno sapiens cDNA done IMAGE:2307163 3' similar to 38 5248 10357 1.5 9.OOE-76 A1652648.1 EST-HUMAN TR:075235 075235 TRAP1 wb30b110.xl NCI_PGAP_GC6 Homo saplens eDNA done IMAGE:2307163 Ysimilar to 38 5248 10358 1.5 9.OOE-76 A1652648.1 EST HUMAN TR:075235 075235 TRAP1; 214 5407 10530 0.9 9.OOE-76 Z45834.1 EST HUMAN HSCZVC061 normalized infant brain cDNA Homo sapiens cDNA done c-zvc06 zi85b07.sl Scares-fetal-live_spleen-1 NFI-S-S1 Horno, saplens cDNA clone 1 MAGE:447541 2364 7466 1.2 9.OOE-76 AA702415.1 EST-HUMAN 3' 923 6070 11227 0.6 8.OOE-76 4504374 NT Homo sapiens H factor 1 (complement) (HFI) mRNA 923 6070 11228 0.6 8.OOE-76 4504374 NT Homo sapiens H factor 1 (complement) (HFI) mRNA 2880 8031 13135 1,1 8.OOE-76 7706724 NT Horno sapiens mediator (Su), mRNA Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex) (DLD) 759 5911 11059 1.4 7.OOE-76 5016092 NT mRNA 3254 8401 13507 2.6 7.OOE-76 AF056490.1 NT Horno sapiens cAMP-specific phosphodiesterase 8A (PIDE8A) mRNA, partial cds 3260 8407 13514 7.1 7.OOE-76 4505052 NT Homo saplens lymphocyte an gen 75 (LY75) mRNA, and translated products Homo sapiens core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cydin D 3293 8439 13549 1.1 7.OOE-76 4757915 NT related (CBFA2T1) mRNA Homo sapiens sepiapterin reductase (7,8-dihydroblopterin:NADP+ oyidoreductase) (SPR) 4275 9400 14483 4.3 7.OOE-76 4507184 NT mRNA Homo sapiens sepiapterin reductase (7,8-dihydrobloptedn:NADP+ oxidoreductase) (SPR) 4275 9400 14484 4.3 7.OOE-76 4507184 NT mRNA 1214 6344 6.7 6.OOE-76 BE396253.1 EST-HUMAN 601312019F1 Nih_1VIG orno sapiens cDNA done IMAGE:3658757 5' 1899 7017 12205 1,3 5.OOE-76 D63874.1 NT Human mRNA for HIVIG-1, complete cds 1899 7617 12206 1.3 5.OOE-76 D63874.1 NT Human mRNA for HIVIGA, complete ods 1899 7017 12207 1.3 5.OOE-76 D63874.1 NT Human mRNA for I-IMGA, complete ods 1 W 6707 11877 1.0 3.OOE-76 4503476 NT Homo sapiens eukaryofic translation elongation factor 1 beta 2 (E1EF1 B2) mRNA Page 128 of 196 Table 4 (Y- r 1581 6707 11878 1.0 3.OOE-76 4503476 NT Homo saplens eukaryofic translaflon elongation factor 1 beta 2 (EEF 1 B2) mRNA 280 5467 10597 1.2 2.OOE-76 D84295.1 NT Human mRNA for possible protein TPRIDII, complete cds 339 5520 10644 1.3 2.OOE-76 D84295.1 NT Human mRNA for possible protein TPRIDII, complete ods 339 5520 10645 1.3 2.OOE-76 D84295.1 NT Human mRNA for possible protein TPIRDII, complete ods 453 5619 0.7 2.OOE-76 4557662 NT Horno saplens immunoglobulin (C1379A) binding protein 1 (IGBPl) mRNA 575 5735 10852 1.1 2.OOE-76 4503944 NT Homo sapiens glucagon (GCG) mRNA 1014 6154 11310 1.0 2.OOE-76 4758053 NT Homo sapiens cAMP responsive element binding protein 1 (CREB1) mRNA 1518 6645 11813 0.6 2.OOE-76 4504028 NT Homo sapiens GM2 ganglioside activator protein (GM2A) mRNA 1518 6645 11814 0.6 2.OOE-76 4504028 NT Homo sapiens GM2 ganglioside activator protein (GM2A) mRNA 1886 7004 12190 0.5 2.OOE-76 AA253954.1 EST - HUMAN zs60hl 1.sl Stratagene schizo brain S1 1 Homo sapiens cDNA clone IMAGE:701925 3' 2811 7962 13068 18 2.OOE-76 P23266 SWISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN F5 3257 8404 13511 2.0 2.OOE-76 AA445992.1 ESTHUMAN zw64e02.sl Soare_t"ds- NHT Homo saplens cDNA done IMAGE:780986 Ysimilar to SW:1TB5-HUMAN P18084 INTEGRIN BETA-5 SUBUNIT PRECURSOR.; 3257 8404 13512 2.0 2.OOE-76 AA445992.1 EST HUMAN zw64e02.sl Soares--- testisNI-IT Homo sapiens cDNA clone IMAGE:780986 Ysimilar to SW:1T135 HUMAN P18084 INTEGRIN BETA-5 SUBUNIT PRECURSOR,; 4043 5467 10597 0.9 2.OOE-76 D84295.1 NT Human mRNA for possible protein TPRIDII, complete cds 4600 9714 14806 4.5 2.OOE-76 4758981 NT Homo sapiens pepfide YY (PYY) mRNA 4867 9974 15059 6.3 2.OOE-76 AW879618.1 EST-HUMAN OV3-OT0028-220300-132- bl 1 OT0028 Homo sapiens cDNA 5068 10166 15242 1.3 2.OOE-76 5031660 NT Homo saplens EGF-like repeats and discoldin 1-like domains 3 (EDIL3), mRNA 4200 9326 14413 2.2 1.OOE-76 D63874.1 NT Human mRNA for HMG-1, complete cds 4200 9326 14414 2.2 1.OOE-76 D63874.1 NT Human mRNA for HMG-1, complete cds 715 5869 11007 0.9 7.OOE-77 AW370405.1 EST-HUMAN RC2-13T0256-241099,01 1402 BT0256 Horno sapiens cDNA 1887 7005 12191 1.2 7.OOE-77 AA625755.1 EST-HUMAN zuglqOl.sl Soares_testis_IS1HT Homo sapiens cDNA done IMAGE:745392 3 2360 7462 12668 0.8 7.OOE-77 4505944 NT Homo sapiens polymerase (RNA) 11 (DNA directed) polypeptide E (25kD) (POLR2E) mRNA 2360 7462 12669 0.8 7.OOE-77 4505944 NT Homo sapiens polymerase (RNA) 11 (DNA directed) polypeptide E (25kD) (POLR2E) mRNA 5449 10575 1.5 6.OOE-77 4M600 NT Homo sapiens interferon (alpha, beta and omega) receptor 2 (IFNAR2) mRNA 1125 6262 11414 0.9 6.OOE-77 AW957753.1 EST-HUMAN EST369823 MAGE resequences, MAGE Homo sapiens cDNA 1524 6651 11819 17.6 6.OOE-77 A1204066.1 EST HUMAN qe77h12.xl Soares- fetaLlung_. NbHL19W Homo saplens cIDNA clone IMAGE:1745063 3' 1216 6346 11505 0,6 5.OOE-77 AF041015.1 NT 7 Homo sapiens glucokinase (GCK) gene, exon 2 1337 6465 11633 1.2 5.OOE-77 4557250 NT Homo sapiens disintegrin and metalloprotease domain 10 (ADAM1 0) mRNA 2736 7820 13024 1.0 5.OOE-77 4503160 NT Horno sapiens cullin 1 LCUL1) mRNA 4605 9719 14812 2.0 5.OOE-77 5031660 NT Homc, saplens EGF-like repeats and discoidin 1-like domains 3 (EDIL3), mRNA 4605 9719 14813 2.0 5.OOE-77 5031660 NT Horno sapiens EGF-like repeats and discoidin 1-like domains 3 (EDIL3), mRNA 4853 9960 150461 2,1 5.0OE-77 AL043953.1 EST HUMAN DKFZP434G172Lrl 434 (synonym: htes3) Homo saplens cDNA done WZp434G1728 V 1859 6977 12162 0.7 4.OOE-77 7706762 NT Homo sapiens villin-like (VILL), mRNA 1929 7046 12237 1.1 3.OOE-77 5730038 NT Homo sapiens SET domain and madner transposwe fusion gene (SETMAR) mRNA lp-l 704(6 122381 1.1 3.OOE-77 5730038 NT Homo saplens SET domain and madner transposase fusion gene (SETMAR) mRNA Page 129 of 196 1 Table 4

3214 8362 13473 6.9 3.OOE-77 A1963236.1 EST-HUMAN wt29c03.xl NCI CGAF_Utl Home sapiens cDNA done IMAGE:2508868 Ysimilar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN); 1414 6542 11717 0.6 2.OOE-77 AW997712.1 EST-HUMAN RC3-BN0053-170200-01 1 - 1101 BN0053 Home sapiens cDNA 2044 7157 12362 0.8 2.OOE-77 L41825.1 NT Homo sapiens CYP17 gene, Cend 2056 7168 12374 1.0 2.OOE-77 7706315 NT Home sapiens CGI-79 protein (LOC51634), mRNA 2549 7946 12844 0.7 2.OOE-77 A13037836.1 NT Home saplens mRNA for KIAA1415 protein, partial cds 2549 7946 12845 0.7 2.OOE-77 AB037836.1 NT Home sapiens mRNA for KIAA1415 protein, partial ods 3305 8450 13560 0.9 2.OOE-77 AF016191.1 NT Rattus norvegicus potassium channel (er93) mRNA, complete cds 3305 8450 13561 0.9 2.0012-77 AF016191.1 NT Rattus norvegicus potassium channel (erg3) mRNA, complete cds 3947 9080 14161 1.3 2.OOE-77 BE044316.1. EST HUMAN ho43b05.xl Soares-NFL- T-G13C-S1 Home sapiens cDNA done IMAGE:3040113 3's m ar to SW:GAG2 HUMAN P10264 RETROVIRUS-RELATED GAG POLYPROTEIN:

4315 9438 14527 0.9 2.OOE-77 A1613519.1 EST-HUMAN tw22g02.xl ISIC1-WAPBm52 Home sapiens cDNA done IMAGE:2260466 3'similar to TR:065245 065245 F211210.7 PROTEIN.; 4315 9438 14528 0.9 2.OOE-77 A1613519.1 EST HUMAN tw22902.xl NCl-WAP-Bm52 Home sapiens cIDNA done IMAGE:2260466 Ysimilar to TR:065245 065245 F21E10.7 PROTEIN.; 4492 9612 3.5 2.OOE-77 4504068 NT Home sapiens glutamic-oxaloacedc transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein, mRNA 4659 9771 14866 3.6 2.OOE-77 AA653025.1 EST HUMAN ns68g12.sl NCI WAP- Pr2 Home sapiens cIDNA done IMAGE:1 188838 similar to SW:W9 HUMAN P47914 60S RIBOSOMAL PROTEIN 1.29. [1j;contains element MSR1 repefifivelement; 37 5246 10353 1.0 1.OOE-77 A13033102.1 NT Home sapiens mRNA for KIAA1276 protein, partial cds 37 5246 10354 1.0 1.OOE-77 A13033102.1 NT Home sapiens mRNA for KIAA1276 protein, partial cds 271 5459 10586 0.6 1.OOE-77 4502166 NT Home saplens amyioid beta (A4) precursor protein (protease nexin-II, Alzheirner disease) (APP), mRNA 271 5459 10587 0.6 1.OOE-77 4502166 NT Home saplens arnylold beta (A4) precursor protein (protease nexin-l I. Alzheimer disease) (APP), mRNA 857 7901 11167 1.7 1.OOE-77 4502166 NT Home saplens amyloid beta (A4) precursor protein (proteast nexin-li, Alzheirner disease) (APP), mRNA 857 7901 11168 1.7 1.OOE-77 4502166 NT Home sapiens arnylold beta (A4) precursor protein (protease nexin-I I. Alzheimer disease) (APP), mRNA 2394 7494 12700 1.2 1.0OE-77 A13029024.1 NT Home saplens mRNA for K1AA1 101 protein, complete cds 3007 8159 13259 2.8 1.OOE-77 4503300 NT Home sapi ns 2,4-dienoyl CoA reductase 1, mitochondrial (DECRI), mRNA 4256 9381 14462 3.0 1.OOE-77 7706299 NT Home saplens CGI-60 protein (LOC51626). mRNA 4423 9543 14635 14.7 1.OOE-77 AJ229041.1 NT Home saplens 959 kb contig between AML1 and CBR1 on chromosome 21q22; segment 113 4552 9671 14761 2.0 1.OOE-77 6552322 NT Home sapiens breast cancer 1, early onset (BRCA1), transcript variant BRCAl-exon4, mRNA 5179 10271 15342 2.9 1.OOE-77 AW755254.1 EST-HUMAN CIVIYA5 Human cardiac muscle expression library Home sapiens cIDNA done 4151935 similar to CMYA5 Cardlomyopathy associated gene 5 -,Z611 12813 1.5 5.OOE-78 AW673424.1 EST HUMAN ba54h03.y3 NIH MGC Home sapiens cDNA done IMAGE:2900405 5'similar to WP:Y48B6A.6 dE22121; Page 130 of 196 Table 4

3339 8483 13598 3.8 5.OOE-78 M55586.11 NT Human collagenase type IV (CLG4) gene, exon 6 4610 9724 14819 1.4 5.OOE-78 AB002348.2 NT Homo sapiens mRNA for KIAA0350 protein, partial cds 1502 6628 11797 0.6 4.OOE-78 AL355841.1 NT Novel human gene mapping to chomosome 22 1629 6755 11925 0.6 4.OOE-78 Ai985O94.1 EST HUMAN wr97bl 2.xl NCI-CGAP- Kidl 1 Homo saplens cDNA clone IMAGE:2495615 X similar to SW:WAP PIG 046655 WHEY ACIDIC PROTEIN PRECURSOR; 2270 7376 12585 0.8 4.OOE-78 AF107405.1 NT Homo sapiens pre-mRNA splicing factor (SFRS3) mRNA, complete cds 4227 9351 14431 1.4 4.OOE-78 7656876 NT Homo saple s syncydn (LOC30816), mRNA 4W 9776 14872 1.3 4.0OE-78 4505806 NT Homo sapiens phosphaddylinositot 4- kinase, catalytic, alpha polypeptide (PIK4CA) mRNA 4664 9776 14873 1.3 4.OOE-78 4505806 NT Horno, sapiens phosphaddAinositol 4-kinase, catalytic, alphA polypeptide (PIK4CA) mRNA 5351 10476 0.6 3.OOE-78 AF095901.1 NT Homo sapiens eRF1 gene, complete cds 5351 10477 0.6 3.OOE-78 AF095901.1 NT Homo sapiens eRF1 gene, co plete cds 3178 8327 13440 0.9 3.OOE-78 4507164 NT HomosaplensnudearanfigenS 100(SP1100)mRNA 4015 8882 13971 0.9 3.OOE-78 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 3083 8234 2.2 2.0012-78 U04489.1 NT Homo sapien tyee W collagen alpha 5 chain (COL4A5) gene, exon 20 3932 9066 1.7 2.OOE-78 AA311872.1 EST HUMAN EST182583 Jurkat T-cells VI Homo sapiens cDNA 9 end 1860 6978 12163 1.8 9.OOE-79 AA601246.1 EST-HUMAN no14909.sl NCI_WAP_Phel Homo sapiens cDNA done IMAGE:1 100704 3'similar to TR:E239140 E239140 SPALT PROTEIN; 4758 9869 14963 2.5 9.OOE-79 BE000837.1 EST HUMAN RC2-BN0074-090300-014- cl 2 BN0074 Horno sapiens cDNA 3682 8821 13914 1.2 8.OOE-79 AL163210.2 NT Horno saplens chromosome 21 segment HS21C010 4391 9513 14601 0.9 8.OOE-79 D28476.1 NT Human mRNA for KIAA0045 gene, complete cds 4391 9513 14602 0.9 8.OOE-79 D28476.1 NT Human mRNA for KIAA0045 gene, complete ods 4805 9915 15007 1.7 8.OOE-79 AF083655.1 NT Homo sapiens procollagen C- proteinase enhancer protein (PCOLCE) gene, 5' flanking region and complete cds 4805 9915 15008 1.7 8.OOE-79 AF083655.1 NT Homo sapiens procollagen C- proteinase enhancer protein (PCOLCE) gene, 5'flanking region and complete cds 3139 8288 1.1 4.OOE-79 8922325 NT Homo saplens hypothetical protein FLJ10283 (FLJ10283), mRNA 310 5494 10623 1.4 3.OOE-79 AF1 14488.1 NT Homo sapiens Intersectin short isoform XITSN) RNA, complete cds 962 6107 11265 0.9 3.OOE-79 AF232708.1 NT Homo sapiens cell-line tsA201a chlodde.lon current Inducer protein I(C1n) gene, complete cds 3060 8211 13310 1A 3.OOE-79 U09410.1 NT Human zinc finger protein ZNF131 mRNA, partial cds 3727 8M 13957 2.8 3.OOE-79 BE265169.1 EST HUMAN 601192921F1 NIH MGC 7 Homo sapiens cIDNA done IMAGE:3536740 5, 4818 9927 108991 5.0 3.OOE-79 AF116627.1 NT Homo sapiens PRO1181 mRNA, complete ods 618 5777 1.1 2.OOE-79 BE379926.1 EST-HUMAN 601159415F2 N11-1_1VIGC 53 Homo saplens cDNA done IMAGE:3511107 5' 912 6058 11217 1.1 2.OOE-79 4757841 NT Homo sapiens BCL2-like 2 (BCL2L2) mRNA 1019 6159 1.4 2.OOE-79 A1523747.1 EST-HUMAN th18h07.xl NCI CGAP Pr28 Homo sapiens eDNA done IMAGE:2118685 X 2101 7212 12420 5.1 2.OOE-79 4585863 NT Homo saple s phosphodlesterase 6A, cGMP-spedfic, rod, alpha (PIDE6A), mRNA 2101 7212 12421 5.1 2.OOE-79 4585863 NT Horno saplens phosphodiesterase 6A, cGMP-sped c, rod, alpha (PIDE6A), mRNA 2146 7256 12463 1,0 2.OOE-79 AJ2714o8.1 NT Homo saplens mRNA for Fas- assodated factor, FAF1 (FaM gene) 0 Page 131 of 196 Table 4

2265 7371 12581 0.6 2.OOE-79 AF244138.1 NT Homo sapiens hepatocellular cardnoma-assodated andgen 88 (HCA88) mRNA, complete cds 3838 8974 14074 0,9 2.OOE-79 AF170492.1 NT Homo sapiens chloride channel CLC4 (C1C4) mRNA, complete cds 4074 9201 14287 1.2 2.OOE-79 A.1271408.1 NT Homo sapiens mRNA for Fas- associated factor, FAF1 (Fafl gene) 4590 9704 14797 1.0 2.OOE-79 AL163206.2 NT Homo saplens chromosome 21 segment HS21C006 204 5397 10525 1.5 1.OOE-79 BE252804.1 ESTHUMAN 601112953F1 N11- 1_1VIGQ_16 Homo saplens cIDNA clone IMAGE:3353581 5' 3107 8257 13360 3.8 9.OOE-80 AA725848.1 EST HUMAN a123c05.sI Soares testis NHT Homo sapiens cDNA done 1343648 3' 3107 8257 13361 3.8 9.OOE-80 AA725848.1 EST-HUMAN a123e0S.sl Soares testis NHT Homo saplens cDNA done 1343648 X 3551 8691 1.0 8.OOE-80 U94387.1 NT Homo sapiens Y chromosome spermatogenesis candidate protein (RBM) pseudogene mRNA, partial cds 882 6030 11189 0.8 6.OOE-80 A1422197.1 EST-HUMAN tf58d02.xl NCLWAP-Bm23 Homo saplens cDNA done IMAGE:2103459 Ysimilar to SW:NUEM-HUMAN Q16795 NADH-UBIQUiNONE OXIDOREDUCTASE 39 KID SUBUNIT PRECURSOR; 1624 6750 11918 0.7 6.OOE-80 U64898.1 NT Homo saplens NRD convertase mRNA, complete ods 4188 9314 14397 1.1 6.OOE-80 A13032981.1 NT Homo saplens mRNA for K1AA1 155 protein, partial ods 4188 9314 14398 1.1 6.OOE-80 A13032981.1 NT Homo sapiens mRNA for K1AA1 155 protein, partial cds 573 5733 10851 1.1 5.OOE-80 4506228 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3) mRNA 817 5967 11124 1.3 5.OOE-80 AF108830.1 NT Homo sapiens sedne-threonine protein kinase (MNBH) mRNA, complete cds 817 5967 11125 1.3 510OE-80 AF108830.1 NT Homo sapiens sedne-threonine protein kinase (MNBH) mRNA, complete cds1172 6306 4.8 5.0012-80 X91647.1 NT H.sapiens ncxl gene (exon 12) 1440 6567 0.8 5.OOE-80 AL163283.2 NT Homo sapiens chromosome 21 segment HS21 C083 2311 7414 12623 0.9 5.OOE-80 U89358.1 NT Human 1(3)mbt protein homolog mRNA, complete cds 2380 7482 12684 1.4 5.OOE-80 AB037855.1 NT Homo sapiens mRNA for KIAA1434 protein, partial cds 2764 7848 13050 1.1 5.OOE-80 4504292 NT Homo sapiens H3 histone family, member J (H3F.1) mRNA 3953 9086 14168 1.1 5.OOE-80 A13019038.1 NT Homo sapiens HMT-1 mRNA for beta-1,4 mannosyltransferase, complete cds 3953 9086 14169 1.1 5.OOE-80 A13019038.1 NT Homo sapiens, HMT-1 mRNA for beta-1,4 mannosyltransferase, complete cds 4872 9979 15063 1.7 5.OOE-80 AL1 63268.2 NT Homo saplens chromosome 21 segment HS21C068 210 5403 1.6 3.OOE-80 AL163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 1308 7912 11604 1.8 2.OOE-80 AA215796.1 EST HUMAN zr98d04xl NCI CGAP GC131 Homo, sapiens cDNA done IMAGE:683719 5' 1308 7912 11605 1.8 2.OOE-80 AA215796.1 EST HUMAN zr98d04xl NCI WAP"GC131 Homo sapiens cDNA done IMAGE:683719 5' 1757 6878 12059 2.3 2.OOE-80 R35321.1 EST HUMAN yg65a08.rl Soares infant brain I NIB Homo sapiens cIDNA clone IMAGE:38060 5' 1816 6935 12125 0,7 2.OOE-80 A1444821.1 EST-HUMAN RET4B7 subtracted retina cIDNA library Homo sapiens cDNA done RET4B7 2009 7123 12323 2.4 2.OOE-80 AL043116.2 EST-HUMAN DI(FZp4341)1323-rl 434 (synonym: htes3) Homo sapiens cDNA clone WZp434131323 5' 337 5518 0.6 1.OOE-80 AL163303.2 NT Homo sapiens chromosome 21 segment HS21 Cl 03 782 5934 11083 1.1 1.OOE-80 AF231920.11 NT Homo sapiens chromosome 21 unknown mRNA 1911 7028 1.1 1.0OE-80 A1732656.1 EST-HUMAN nnOlfl2.x5 NCI_PGAp_pog Homo sapiens cIDNA done IMAGE:1076495 Ysimilar to contains OFR.M OFR repetitive element; 3515 8656 13763 1.0 1.OOE-80 8922391 NT Homo saplens hypothetical protein FLJ10379 (FL.110379), mRNA 4 9480 14571 1.0 1.OOE-80 AF077188.1 NT Homo sapiens cullin 4A (CUL4A) mRNA, complete cds Page 132 of 196 Table 4

1^ C 5192 102F3 15351 1.0 11.0OE-80 A1.163278.2 NT Homo, sapiens chromosome 21 segment HS21 C078 4290 9414 14503 4.8 6.OOE-81 BE256829.1 EST-HUMAN 601111970F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE:3352840 5' 4290 9414 14504 4.8 6.OOE-81 BE256829.1 EST-HUMAN 601111970F1 N1H MGC 16 Homo saplens cDNA done IMAGE:3352840 5' 2170 7279 12492 1.1 5.00FE-81 BE268042.1 ESI_HUMAN 601 125505F1 N1H MGCJ Homo sapiens cDNA done IMAGE:3345480 5' 1779 6900 12088 1.0 4.OOE-81 AW779612.1 EST-HUMAN hn98d02.xl NCI_CGAP_Col4 Horno saplens cDNA done IMAGE:3035907 Wsimilar to SW:COPG-BOVIN P53620 COATOMER GAMMA SUBUNIT; 2058 7170 12377 2.1 4.0OE-811 8923056 NT Homo sapiens hypothetical protein FLJ20048 (FI.J20048), mRNA 2058 7170 12378 2.1 4.OOE-81 8923056 NT Homo saplens hypothetical protein FLJ20048 (F1J20048), mRNA 3134 8283 13390 3.8 4.OOE-81 A13037766.1 NT Homo sapi6ns mRNA for K1AA1 345 protein, partial cds 3576 8715 13812 1.2 4.OOE-81 AW004608.1 EST-HUMAN ws9OhO3,xl NCI-CGAR_CW Homo sapiens cDNA clone IMAGE:2505269 3'similar to TR:043815 043815 STRIATIN.

1246 6375 11532 3.2 3.OOE-81 Y18000.1 NT Homo sapiens NF2 gene 1246 6375 11533 3.2 3.OOE-81 Y18000.11 NT Homo sapiens NF2 gene 2320 7423 12632 0.6 3.0OE-811 AF077188.1 NT Homo saplens cullin 4A (CUL4A) mRNA, complete cds 2961 8112 13217 5.1 3.0OE-811 4506280 NT Homo sapiens plelotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) (PTN) mRNA 2961 8112 13218 5.1 3,OOE-81 4506280 NT Homo sapiens pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) (PTN) mRNA 4933 10039 0.9 3.OOE-81 AL163283.2 NT Horno saplens chromosome 21 segment HS21C083 3707 8845 13936 1.0 2.OOE-81 AW611542.1 EST UMAN hg85c01.xl NCI CGAP Kidl 1 Horno sapiens cDNA clone IMAGE:2952384 3 4579 9695 14788 0.9 2.OOE-81 5453871 NT Homo sapiens platelet-derived growth factor receptor-like (PDGFRL) mRNA 1403 6530 11701 1.2 1.OOE-81 W26539.1 EST HUMAN 33M Human retina cDNA randomly primed sublibrary Homo sapiens cIDNA 2144 7254 12462 0.9 1.OOE-81 N66951.1 EST HUMAN za48f12.sl Soares fetal liver spleen INFLS Homo sapiens cDNA clone IMAGE.2958233' 3602 8741 13836 2.0 1.OOE-81 AW960658.1 EST-HUMAN EST372729 MAGE resequences, MAGF Homo sapiens cDNA 4414 9534 14623 3.1 1.0OE-81 AA040370.1 EST-HUMAN zk45h09xl Soares_pregnanLuterus-NbHPU Homo sapiens cDNA done IMAGE:485825 5' similar to PIR:S52437 S52437 CDP-diacyiglycerol synthase - fruit fly; 4546 9665 14754 7.9 1.OOE-81 BE047996.1 EST-HUMAN tz45c04y11 NCI CGAP 13m52 Horno sapiens cIDNA done IMAGE:2291526 5' 12 5220 10321 0.6 8.OOE-82 AF161406.1 NT Homo sapiens HSPC288 mRNA, partial eds 101 5220 10321 1.3 8.OOE-82 AF161406.1 NT Homo sapiens HSPC288 mRNA, partial ods 263 5450 10576 0.6 8.0012-82 U08988.1 NT Human CRFB4 gene, partial cels 797 5948 11098 0.7 8.OOE-82 U08988.11 NT Human CRFB4 gene, partial cds 869 6017 11177 1.1 8.OOE-82 U08988.11 NT Human CRFB4 gene, partial ods 1475 6602 11776 1.1 8.OOE-82 AB037748.1 NT Homo sapiens mIRNA for KIAA1327 protein, partial cds 1636 6762 11932 1.2 8.OOE-82 6715601 NT Homo sapiens glutathione peroAdase 5 (epididymal androgen-related protein) (GPXS), transcript variant 2, mRNA 4150 9276 14357 -0,8 8.OOE-82 8923432 NT Homo sapiens hypothetical protein FU20461 (FL.120461), mRNA 4034 9162 14245 0.8 5.OOE-82 AA515512.1 ESTHUMAN nf69el 1.s11 NCI CGAP CO Homo sapiens cDNA done IMAGE:925196 X 1647 677 11 2t 2.4 -4.OOE-82 AF081484.1 NT Homo sapiens alpha-tubulln Isoform 1 mIRNA, complete cds 4638 9752 14845 0.8 4.OOE-82 H17755.1 EST_HUMAN ym40e08xl Soares Infant brain 1 NIB Homo sapiens cDNA done IMAGE:50499 5' ii:. 9752 14846 0.8 4.OOE-82 H17755.1 ES7_HUMAN yM40eO8.rl Scares infant brain 1 NIB Homo sapiens cDNA done IMAGE:50499 6 Page 133 of 196 1 Table 4 -t- p.1 276 5464 10593 4.7 3.OOE-82 4502166 NT Homo saplens amyloid beta (A4) precursor protein (protease nexin-fl, Alzheirner disease) (APP), mRNA 687 5842 10978 0.8 3.0012-82 BE005705.1 EST-HUMAN RC2-BN0120-010400- 013402 BN0120 Homo sapiens cDNA 703 5058 1.0 3.0012-82 4502506 NT Homo sapiens complement component 5 (C5) mRNA 770 5922 11071 1.5 3.OOE-82 5174702 NT Homo sapiens transforming growth factor beta-activated kinase-binding protein. 1 (TAB1), mRNA 852 6001 11161 1.1 3.OOE-82 4502166 NT Homo sapiens arnyloid beta (A4) precursor protein (protease nexin-l I, Akheimer disease) (APP), mRNA 1045 6185 4.7 3.OOE-82 AA725848.1 EST-HUMAN a123c05.sl Soares_testis NHT Homo sapiens cDNA done 1343W 3, 1333 6462 11630 5.5 3.OOE-82 AW875073.1 EST - HUMAN RC6-PT0001-190100-021- BO2 PT0001 Homo sapiens cDNA 1450 6577 11751 0.7 3.OOE-82 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 1961 7077 12276 0.9 3.0012-82 4501922 NT Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type 1 (ADCYAP1R1)mIRNA 3234 8382 2.1 3.OOE-82 5453811 NT Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) mRNA 4836 9944 15033 0.9 3.OOE-82 AA1 35979.1 ESTHUMAN zn93b04xl Stratagene lung carcinoma 937218 Homo sapiens ONA done IMAGE:565711 5, similar to SW:PAGI_BOVIN Q07537 POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE; 582 5742 10858 0,7 2.OOE-82 A13023216.1 NT Homo sapiens mRNA for KIAA0999 protein, partial cds 582 5742 10859 0.7 2.OOE-82 AB023216.1 NT Homo saplens mRNA for KIAA0999 protein, partial ods 1660 6784 11960 0.6 2.0012-82 AL046390.1 EST-HUMAN DKFZp434M1 17-rl 434 (synonym: htes3) Homo saplens cDNA done DKFZp434M1 17 5, 2176 7285 12496 1.1 2.OOE-82 5453855 NT Homo sapiens pericentriolar material 1 (PCM1) mRNA 3231 8379 1,8 2.OOE-82 6997248 NT Homo sapiens sal (Drosophila)-like 1 (SALL1), mRNA 3772 8909 13998 1.1 2.OOE-82 D87675.1 NT Homo saplens DNA for amyloid precursor protein, complete cds 4140 926 14347 1.1 2.OOE-82 4504116 NT Homo sapiens glutamate receptor, lonotroplc, kainate 1 (GRIK1) mRNA 4458 9578 14675 1,0 2.OOE-82 AB02901 9.1 NT Homo sapiens mRNA for KIAA1096 protein, partial cds 4458 9578 14676 1.0 2.0OE-82 AB029019.1 NT Homo saplens mRNA for KIAA1096 protein. partial cds 1 4768 9879 14973 3.2 2.0012-82 AF045555.1 NT Homo saplens wbscrl (WBSCR1) and wbscr5 (Wi3SCR5) genes, complete cds, alternatively spliced and replication factor C subunit 2 (RlFC2) gene, complete cds 5013 10115 15185 1.7 2.OOE-82 4507580 NT Homo sapiens tumor necrosis factor receptor superfamily, member 5 (TNFRSF5) mRNA 5013 10115 15186 1.7 2.OOE-82 4507580 NT Homo. sapiens tumor necrosis factor receptor superfamily, member 5 (TWRSF5) mRNA 1264 6393 11552 0.7 1.OOE-82 BE064386.1 EST-HUMAN RC4-13T0310-110300- 015410 BT0310 Homo sapiens cDNA 1265 6394 11553 0.8 1.OOE-82 AB01 1110.2 NT Homo sapiens mRNA for KIAA0538 protein, partial ods 1392 6519 11687 0.7 8.OOE-83 BE383973.1 EST_HUMAN 601273346F1 N1H MGC 20 Homo saplens cDNA clone IMAGE:3614362 5' 1656 7869 11954 0.7 8.OOE-83 N66951.1 EST-HUMAN za48f12.sI Soares fetal liverspleen I NFLS Homo saplens cDNA done IMAGE:295823 3 2836 7987 1.6 7.OOE-83 AA584655.1 EST-HUMAN no12h011.sI NCI - CGAP Phel Homo saplens cDNA done IMAGE:1 100497 Ysimilar to contains Su repetitive ilement 1 15055 8.7 7.OOE-83 N27808,11 EST_HUMAN e09.rl Soares melanocyte 2NbHM Homo saplens cDNA done IMAGE:265576 5' Page 134 of 196 Table 4

V1 397 5565 10701 1.3 6.OOE-83 M33320.1 NT Human platelet Glycoprotein lib (GPI1b) gene, exons 2-29 1747 6869 12047 0.5 6.OOE-83 AW573088.1 EST-HUMAN hf3 1 h03.xl Soares NFL, GBC S 1 Homo sapiens cDNA done IMAGE:2933525 3'similar to SW:YBE13 HAEIN P44471 HYPOTHETICAL PROTEIN H10034.

2984 8136 13241 1.1 6.OOE-83 AW816405.1 EST-HUMAN OV4-ST0234.181199-037f05 ST0234 Homo sapJens cDNA 3031 8183 13283 0.9 6.OOE-83 AA701457.1 EST-HUMAN z159c05.sl Soaresfetal- liver-spleer!_1NFLS-S1 Homo saplens d3NA done IMAGE:435080 3 931 6078 1.4 5.OOE-83 U17883.1 NT Human succinate dehydrogenase iron- protein subunit (OhB) gene, exon 5 2004 7871 0.8 5.OOE-83 AF006305.1 NT Homo sapiens 26S proteasome regulatory subunit (SUG2) mRNA, complete ods 3586 8725 13821 0.9 5.OOE-83 AL133207.2 NT Novel human gene mapping to chomosome X 5011 10113 15183 11.0 5.0012-83 4557013 NT Homo sapiens catalase (CAT) mRNA 5011 10113 15184 11.0 5.OOE-83 4557013 NT Homo sapiens catalase (CAT) mRNA 5094 10192 15271 0.9 5.OOE-83 AF083827.11 NT Homo sapiens dihydropy#oine receptor alpha 2 subunit ((5CNA2D1) gene, exon 11 624 5783 10905 1.5 4.OOE-83 AF224669.1 NT Homo sapiens mannosidase, beta A, lysosomal (MANBA) gene, and ubiquidn-conjugafing enzyme E2D 3 (LIBE2D3) genes, complete cds 981 6126 1.5 3.OOE-83 AA36831 1.1 EST-HUMAN EST79542 Placenta 1 Homo sapiens cIDNA similar to similar to endogenous retrovirus ERVg 2750 7834 0.7 3.OOE-83 AA632654.1 EST-HUMAN rip87c07.sl NCI-CGAP-Thyl Homo sapiens cDNA done IMAGE:1 133292 similar to contains THR.t2 THR repetitive element; 1759 6880 12061 0.7 2.OOE-83 AA993492.1 EST-HUMAN ot64905.sl Soares_tesds NFIT Homo sapiens cIDNA done IMAGE:1621592 3'similar to TR:Q92614 092614 MYELOBILAST K1AA0216.; 1759 68 0 12062 0.7 2.OOE-83 AA993492.1 EST HUMAN ot64g05.sl Soares--- testis - NHT Homo saplens cIDNA done IMAGE: 1621592 Ysimilar to TR:Q92614 Q92614 MYELOBLAST K1AA0216.; 1883 7001 12186 0,8 2.OOE-83 N66951.1 EST HUMAN za48f12.si Soares fetal liver spleen 1 NFLS Homo sapiens cDNA done IMAGE:295823 3, 1983 7098 12301 1.1 2.OOE-83 AW247459.1 EST - HUMAN 2819246.5prime N1H MGC 7 Homo sapiens cIDNA done IMAGE:2819246 5, 3708 8846 1.0 2.OOE-83 AL1 63202.2 NT Homo sapiens chromosome 21 segment HS21C002 4241 9366 14447 4.5 2.OOE-83 AF202879.1 NT Homo sapiens hematopoietic progenitor cell antigen CD34 precursor (CD34) mRNA, partial cds 4553 9672 14762 8.1 2.OOE-83 7706398 NT Homo sapiens ankydn repeat- containing protein AS13-2 (LOC51676), mRNA 4553 9672 14763 8.1 2.OOE-83 7706398 NT Homo sapiens ankyfin repeat- containing protein AS13-2 (LOC51676), mRNA 1273 6402 11560 1.4 1.OOE-83 4505492 NT Homo sapiens oxoglutarate dehydrogenase (lipoamide) (OGDH) mRNA 1273 6402 11561 1.4 1.OOE-83 4505492 NT Homo sapJens oxoglutarate dehydrogenase (lipoamlde) (OGDH) mRNA 1390 6517 11684 6.0 1.OOE-83 4504326 NT Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolaselenoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (HADHB) mRNA 1390 6517 11685 6,0 1.0OE-83 4504326 NT Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolaselenoyi-Coenzyme A hydratase (trifunctional protein), beta subunit (HADH13) mRNA 8295 13404 0.8 1.OOE-83 7662349 NT Homo sapiens cell recognition molecule Caspr2 (K1AA0868), mRNA 3793 8930 14023 3.6 1.OOE-83 AF053768.1 NT Rattus noryegicus brain specific cortactin-binding protein CBP90 mRNA, partial cds i3146 " 92 14358 2.2 1.001 - ------^. ondriall dodecenoyl-CoA delta-isomerase, exon 3 Page 135 of 196 1 Table 4 j 9 4789 9909 14993 1,4 1.OOE-83 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-l I, Mzheimer disease) (APP), mRNA 2348 7450 12657 1.7 6.OOE-84 AA776574.1 EST-HUMAN ae86a03.sl Stratagene schizo brain SI 1 Homo sapiens cDNA done IMAGE:971020 3, 697 5852 10988 1.1 5.0012-84 AA38281 1.1 EST HUMAN EST96094 Testis 1 Homo sapiens cDNA Vend 2981 8132 1.0 5.OOE-84 AF109718.1 NT Homo sapiens chromosome 3 subtelomeric region 1354 6482 11648 1.1 4.0012-84 A13037735.1 NT Homo saplens mRNA for K1AA1 314 protein, partial ods 1389 6516 11683.1.5 4.OOE-84 A1685321.1 EST HUMAN wa76c04.xl Soares - NFL, GBC-Sl Homo sapiens cDNA done IMAGE:2302086 Wsimilar to SW:NRDC HUMAN 043847 NARDILYSIN PRECURSOR; 4869 9976 15061 1.8 4.OOE-84 AF069601.2 NT Homo saplens myosin light chain kinase Isoform 2 (MI.CK) mRNA, complete ods, 5060 10158 15233 1.3 4.OOE-84 U94982.1 NT Human 2,4-dienoyi-CoA reductase gene, exons 3 and 4 313 5497 10626 1.4 3.OOE-84 AF026200.1 NT Homo sapiens Bachl protein homolog mRNA, partial cds 1137 6273 11423 0.9 3.OOE-84 4758081 NT Horno sapiens chondroldn sulfate proteoglycan 2 (versican) (CS1PG2) mRNA 1917 7034 12222 1.2 3.OOE-84 5453855 NT Homo sapiens pericentdolar material 1 (P M1) mRNA 1962 7078 12277 1.1 3.OOE-84 A1-096880.1 NT Novel human mRNA containing Zinc finger C2H2 type domains 3542 8683 13783 1.2 3.OOE-84 A13026898.1 NT Homo sapiens DNA, DLECI to ORCTL4 gene region, section 112 (DI-EC1, ORCTO, ORCTL4 genes, complete cds) 3689 8827 13921 4.1 3.OOE-84 AF014459.1 NT Homo saplens X-linked juvenile retinoschisis precursor protein (XL.RS1) mRNA, complete cds 2913 8064 13172 9.6 2.0OE-84 AF036943.1 NT Homo saplens myelin transcription factor 1 -like (MYTI1-1) mRNA, complete cds 2932 8083 13191 0.9 2.OOE-84 X8921 1.1 NT H.saplens DNA for endogenous retroviral like element 309 5493 10622 0.5 1.OOE-84 AF1 14488.1 NT Horno sapiens intersectin short isoform (ITSN) mRNA, complete ods 537 5700 10822 1.8 1.OOE-84 4507952 NT Homo sapiens tyrosine 3monooxygenaseltryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) mRNA 1271 6400 11568 0.7 1.OOE-84 AA984379.1 EST-HUMAN am85b11.s1 Stratagene schizo brain.Sl l Homo sapiens cDNA clone IMAGE:1629885 3, 2008 7122 12322 1.1 1.OOE-84 BE392137.1 EST HUMAN 601308006F1 N1H MGC 44 Homo sapiens cDNA done IMAGE:3626257 5, 3691 8830 13923 2.1 1.OOE-84 AA720851.1 EST-HUMAN nwi 2e06.sl NCI CGAP SS1 Homo saplens cDNA clone IMAGE:1239106 3 4319 9442 14530 5.6 1.OOE-84 AJ229041.1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22; segment 113 4601 9715 14807 3.8 1.OOE-84 AL043314.2 EST-HUMAN DKFZp434N0323-rl 434 (synonym: htes3) Homo sapiens cDNA clone DKFZp434N0323 T 4601 9715 14808 3.8 1.OOE-84 AL043314.2 EST HUMAN DKFZp434N032_rl 434 (synonym: htes3) Horno sapiens cDNA done DKRp434N0323 5' 4823 9442 14530 4.3 1.0012-84 AJ229041.1 NT Homo sapiens 959 kb config between AMLI and CBR1 on chromosome 211q22; segment 113 951 6097 1.2 9.0012-85 AL163209.2 NT Homo sapiens chromosome 21 segment HS21 C009 1057 6196 11348 0.8 9.OOE-85 U51432.1 NT Homo saplens nuclear protein Skip mRNA, complete cds 1057 6196 11349 0.8 9.OOE-85 U51432.1 NT Homo sapiens nuclear protein Skip mRNA, complete ods 1560 6687 11854 1.1 9.OOE-85 M33282.1 NT minogen gene, exon 7 1560 6687 11855 1.1 9.OOE-85 M33282.1 NT Human plasminogen gene, exon 7 AEns 6776 11949 1.8 9.OOE-85 7657020 NT DKFZp434P211 protein (DKFZP434P21 1), mRNA Homo saple Page 136 of 196 Table 4

4158 9284 14364 0.9 9.OOE-85 A1-163280.2 NT Homo saplens chromosome 21 segment HS21 C080 4778 9888 14981 1.1 9.OOE-85 5901979 NT Homo sapiens heat shock transcription factor 2 binding protein (HSF2BP), mRNA 4825 9933 15023 1.0 9.0OE-85 AL163268.2 NT Homo sapiens chromosome 21 segment HS21C068 1120 6257 11409 1.1 7.OOE-85 L05094.1 NT Homo sapiens ribosomal protein L27 mRNA, complete cds 2285 7390 12598 1.2 5.OOE-85 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 2530 7626 12827 1.1 5.OOE-85 F08331.1 EST-HUMAN HSCZQH081 normalized Infant brain cDNA Homo sapiens cDNA done 97NhO8 2530 7626 12828 1.1 5.OOE-85 F08331.1 EST HUMAN HSCZQH081 normalized Infant brain cDNA Homo sapiens cl3NA clone czqhO8 1277 6407 11567 1.2 3.OOE-85 AF096157.1 NT Homo sapiens protein phosphatase 2A BR gamma subunit gene, exon 6 1741 6864 12041 1.2 3.OOE-85 T97495.1 EST-HUMAN ye53909.rl Soares fetal liver spleen 1NFLS Horno sapiens cDNA clone IMAGE:121504 5' 4219 9343 14426 0.9 3.OOE-85 BE267189.1 EST HUMAN 601189704F2 N1H MGC 7 Homo saplens cDNA done IMAGE:3533616 5' 4883 9991 15072 0.9 3.OOE-85 7363442 NT Homo sapiens olfactory receptor, family 12, subfamily D, member 2 (OR12D2), mRNA 947 6093 11251 0,9 2.OOE-85 7657266 NT Homo saplens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog (K1AA0929), mIRNA 1024 6164 11318 0.6 2.OOE-85 AF248540.1 NT Homo sapiens Intersectin 2 (SH3D1 B) mRNA, complete cds 1400 6527 11696 2.6 2.OOE-85 5174775 NT Homo sapiens apolipoprotein C-11 (APOC2) mRNA 1400 6527 11697 2.6 2.OOE-85 5174775 NT Homo sapiens apolipoprotein C-11 (APOC2) mRNA 2183 7292 12504 0.8 2,OOE-85 U10525.11 NT Human DNA polymerase beta gene, exons 12 and 13 2793 6444 1.4 2.OOE-85 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121LI), mRNA 4239 9364 14445 5.4 2.OOE-85 4505880 NT Homo sapiens plasminogen (PLG) mRNA, 4822 9951 15021 1.3 2.OOE-85 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 2241 7347 0,9 1.0012-85 BE277489.1 EST-HUMAN 601179190F1 N1H MGC 20 Homo sapiens cDNA done IMAGE:3546959 9 1410 6537 2.8 9.OOE-86 BE274217.1 EST-HUMAN 601 120778F1 Nib1VIGQ_20 Homo saplens cDNA clone IMAGE:2967690 S' 220 5412 10533 1.3 7.OOE-86 7662247 NT Horno sapiens KIAA0680 gene product (K1AA0680), mRNA 921 6067 11223 1.3 7.OOE-86 AA860801.1. ES-_HUMAN aj88108.sl Soares_parathyroldjumor-NbHPA Homo sapiens cDNA clone IMAGE:1403559 3' 921 6067 11224 1.3 7.00E786 AA860801.1 EST-HUMAN aj88fO8.sl Soares_parathyroid_tumorNbHPA Homo sapiens cDNA done IMAGE:1403559 X 1272 6.401 11559 3.2 6.OOE-86 4505492 NT Homo sapiens oxoglutarate dehydrogenase (lipoamide) (OGDH) mRNA 4380 9502 3.2 6.OOE-86 8923209 NT Homo sapiens hypothetical protein FLJ20220 (FU20220), mRNA 266 5453 10578 1.3 2.OOE-86 AA306264.1 EST HUMAN EST177232 Jurkat T-cells V1 Horno sapiens cDNA Vend 408 5576 0.6 2.0012-86 AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 1173 6307 11461 0.7 2.OOE-86 N58977.1 EST-HUMAN yzl9aO8.rl Soare_muidpi_scJerosis-2NbHMSP Horno sapiens d3NA done IMAGE:283478 5' 2222 7329 12543 1.1 2.OOE-86 AB033103.1 NT Homo sapiens mRNA for KIAA1277 protein, partial ods 3369 8512 13619 1.4 2.OOE-86 AW966142.1 EST-HUMAN EST378215 MAGE resequences, MAGI Horno saplens cDNA 3686 8824 13917 2.2 2.OOE-86 AF156776.1 NT Homo sapiens lysophosphaddic add acyttransferascAelta (LPAAT-delta) mIRNA, complete cds L__3680-C24L_13918L 2.2 2.OOE-86 AF156776.1 NT Homo, sapiens lysophosphaddic add acyltransferase-delta (LPAAT-delta) mRNA, complete cds Page 137 of 196 Table 4 (>0 C.

3954 9087 2.4 2.OOE-86 AW515742.1 EST-HUMAN W87g08.xl NCI CGAP GC6 Horno sapiens cDNA done IMAGE:2916542 3' 4679 9791 14692 2.8 2.OOE-86 AF056490.1 NT Homo sapiens cAMP-spedfic phosphodiesterase 8A (PDE8A) mRNA, partial ods 1579 6705 11875 1.1 1.0OE-86 4826855 NT Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme 0 reductase) (NDUFS1) mRNA 3125 8274 13379 2.1 1.0012-86 5453649 NT Homo sapiens fibulin 5 (FBI-N5) mRNA 3197 8346 13459 2,4 1.0OE-86 L20492.1 NT Human gamma-glutarnyl transpeptidase mRNA, complete cds 3256 8403 13509 1.3 1.OOE-86 AL1632097 NT Homo sapiens chromosome 21 segment HS21 C009 3256 8403 13510 1.3 1.0OE-86 A1163209.2 NT Horno sapiens chromosome 21 segment HS21 C009 3864 9000 14100 11.5 1.0OE-86 7706161 NT Homo sapiens hypothetical protein (LOC51318), mRNA 3864 9000 14101 11.5 1.OOE-86 7706161 NT Horno sapiens hypothetical úrotein (LOC51318), mRNA 4167 9293 14374 5.8 1.OOE-86 AL1 63300.2 NT Horno sapiens chromosome 21 segment HS21C100 4832 9940 15029 1.1 1.OOE-86 AF100751.1 NT Horno saplens FK506-binding protein FK13231soform mRNA, complete cds 471 5636 10765 4.8 8.OOE-87 X62245.1 NT O.cuniculus mRNA for elongation factor 1 alpha 3482 8623 13735 0.9 6.OOE-87 7657213 NT Homo sapiens hormonally upregulated neu turnor-assodated kinase (HUNK), mRNA 1142 6278 11430 0.6 5.OOE-87 AA38281 1.1 EST_HUMAN EST96094 Testis 1 Horno sapiens cDNA Tend 950 6096 11253 1.3 4.OOE-87 AL163210.2 NT Homo saplens chromosome 21 segment HS21C010 1155 6295 11444 3.6 4.OOE-87 A13037835.1 NT Horno sapiens mRNA for KIAA1414 protein, partial cds 1411 6538 11711 0.9 4.0OE-87 R78133.1 EST HUMAN yi80f1O.rl Soares placenta Nb21-1P Homo sapiens cDNA clone IMAGE:145579 5' similar to contains Alu repedlive element; 1985 7100 12303 0.9 4.0OE-87 AB007925.1 NT Homo sapiens rnRNA for KIAA0456 protein, partial cds 2372 7474 12674 1.1 4.0012-87 7706299 NT Homo sapiens CGI-60 protein (LOC51626), mRNA 2372 7474 12675 1.1 4.OOE-87 7706299 NT Homo sapiens CGI-60.protein (LOC51626), mRNA 3419 8561 13672 2.2 4.OOE-87 5174574 NT Homo sapiens myeloidfiymphold or mixed-fineage leukernla (trithorax (Drosophila) homolog); translocated to, 4 (Mi-LT4) mRNA 2749 7833 13034 1.1 2.OOE-87 4885420 NT Horno sapiens high-m6Wity group (nonhistone chromosomal) protein 4 (HIvIG4) mRNA 4880 9987 15069 0.8 2.OOE-87 BE175478.1 EST-HUMAN RCS-HT0580-200300-031- GO4 HT0580 Horno saplens cDNA 1165 7867 0.8 1.0OE-87 7705683 NT Homo sapiens putative glycolipid transfer protein (LOC51054), mRNA 1413 6540 11713 0.9 1.0OE-87 AW361977.1 EST HUMAN PM2-CTO265-141099-001- 904 CT0265 Homo sapiens cDNA 1413 6540 11714 0.9 1.OOE-87 AW361977.1 EST HUMAN PM2-CT0265-141099-OF- 904 CT0265 Horno sapiens cDNA 3649 8788 13881 3.2 1.0OE-87 Y00052.1 NT Human mRNA for T-cell cyclophilin 3673 8812 13906 2.5 1.0OE-87 4758827 NT Homo s piens neureAn Ill (NRXN3) mRNA 5057 10155 15231 1.0 1.OOE-87 U50949.1 NT Rattus norvegicus taste bud receptor protein TB 641 (TB 641) gene, complete cds 1090 6278 11379 2.5 9.OOE-88 AF167465.1 NT Homo saplens double stranded RNA activated protein kinase (RKR) gene, exon 12 1327 6459 11622 0.7 9.OOE-88 AB037820.1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 1327 6456 11623 0.7 9.OOE-88 AB037820.1 NT Horno sapiens mRNA for K1AA1 399 protein, partial cds 3574 8713 13811 1.1 9.OOE-88 AL1 63209.2 NT Horno sapiens chromosome 21 segment HS21 C009 4172 9298 14379 2.6 9.OOE-88 X91929.1 NT H.sapiens ECE-1 gene (exon 9)_ 4172 9298 14380 2.6 9.OOE-88 X91929.1 NT H. CE-1 gene (exon 9) 4934 10040 15112 1.2 9.OOE-88 AB026898.1 NT Horno sapiens DNA, DLECI to ORCTL4 gene region, section 112 (DLECI, ORCTO, ORCTI-4 genes, complete ' cds) 1787 6907 1.2 5.OOE-88 7661887 NT Horno sapiens KIAA0063 gene product (K1AA0063), mRNA Page 138 of 196 Table 4 c C 2602 7692 12898 1.1 5.OOE-88 N89399.1 EST-HUMAN K971 9F Human fetal heart. Lambda ZAP Express Homo saplens cDNA done K971 9 S' similar to ZINC FINGER PROTEIN HW1 2970 8121 13228 0.9 5.OOE-88 AF1 14488.1 NT Homo sapiens intersectin short Isoform (ITSN) mRNA,.complete cds 2978 8129 13236 0.9 5.OOE-88 AF1 14488.1 NT Homo sapiens Intersectin short isoform_(ITSN) mRNA, complete cds 2978 8129 13237 0.9 5.OOE-88 AF1 14488.1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 3343 8487 2.3 5.OOE-88 A1693217.1 EST_HUMAN M68h08.xl NCI_PGAP_Lu24 Homo sapiens cDNA done IMAGE:2336799.3'similar to contains Alu repetitive element;contalns element MER22 MER22 repetitive element; 3491 8632 13744 0.9 5.OOE-88 AF1 14488.1 NT Homo sapiens Intersectin short Isoform (ITSN).miRNA, complete cds 4631 9745 14842 0,9 5.0012-88 AF1 14488.1 NT Horno sapiens intersectin short isoform (ITSN) mRNA, complete cds 207 5400 10527 1.5 3.OOE-88 4826831 NT Homo sapiens Machado-Joseph disease (spinocerebellar atayJa 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) (MJD) mIRNA 1770 6891 4.8 3.OOE-88 4508020 NT Homo sapiens zinc finger protein 259 (gNF259) mRNA 2918 8069 13179 4.3 3.OOE-88 N66951.1 ESIHUMAN za48f12.al Scares fetal liver spleen INFLS Homo sapiens LDNA clone IMAGE:295823 3' 4147 9273 14351 1.2 3.OOE-88 4501912 NT Homo sapiens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 4147 9273 14352 1.2 3.0OE-88 4501912 NT Homo soplens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 1020 6160 11312 1.4 2.OOE-88 7305198 NT Homo sapiens Calsenitin, presenilin-binding protein, EF ha.nd transcription factor (C5EN), mIRNA 1607 6734 11904 0.9 2.OOE-88 AF24621 9.1 NT Horno saplens SNARE protein kinase SNAK mRNA, complete cds 1716 6839 12018 1.5 2.OOE-88 AF246219.1 NT Horno sapiens SNARE protein kinase SNAK mRNA, complete cds 4328 9451 14539 1.9 2.OOE-88 5031666 NT Homo sapiens dyncin, axonemal, light polypeptide 4 (DNAL4), mRNA 1710 6833 12013 0.6 9.OOE-89 4759149 NT Horno sapiens SIVIC (mouse) homolog, Y chromosome (SIVICY) mRNA 2705 7790 12991 0,5 8.0OE-89 BE311557.1 EST-HUMAN 601142409F1 NIH_MG.14 Homo sapiens cDNA done IMAGE:3506186 5, 426 5593 10726 1.4 7.0012-89 7657213 NT Horno sapiens hormonally upregulated nou tumor-assodated kinase (HUNK), mRNA 426 5593 10727 1.4 7.OOE-89 7657213 NT Homo sapiens hormonally upregulated neu tumor-assodated kinase (HUNK), mRNA 4785 9896 14989 2.9 7.0012-89 4557390 NT Homo sapiens complement component 8, beta polypepfide (C813) mIRNA 4847 9954 15043 5.1 7.OOE-89 AL045748.1 EST-HUMAN UFZp434E24Q_rl 434 (synonym: htes3) Homo sapiens cDNA done DKI7Zp434E246 5' 1006 65-6 11300 1.1 6.0012-89 5803114 NT Homo sapiens Inner membrane protein, mitochonddal (mitofilin) (IMMT), mRNA 1601 6728 11898 1.9 6.OOE-89 4827015 NT Horno sapiens solute carrier family 4, anion exchanger, member (SLC4A3) mRNA 1601 6728 11899 1.9 6.0OE-89 4827015 NT Homo sapiens solute carrier family 4, anion exchanger. member 3 (SLC4A3) mRNA 2166 7275 12486 1.1 6.OOE-89 4506124 NT Horno saplens serinelthreonine- protein kinase PRP4 homolog (PRP4) mRNA 2383 7485 12686 0.7 6.OOE-89 4507788 NT Homo saplens ubiquidn-oonjugadng enzyme E2L 3 (U131221-3) mRNA 2383 7485 12887 0.7 6.OOE-89 4507788 NT Homo sapiens ubiquidn-conjugafing enzyme E2L 3 (UBE2L3) mRNA 3480 8621 13733 0.8 6.OOE-89 7661817 NT Homo sapiens HSPC1 59 protein (I- ISPC1 59), mRNA 4537 9656 14743 3.5 6.OOE-89 AB007866.2 NT Homo sapiens mIRNA for KIAA0406 protein, partial cds 4537 9656 14744 3.5 6.OOE-89 AB007866.2 NT Horno saplens; mRNA for KIAA0406 protein, partial ods 5007 09 101115178 2.8 5.OOE-89 BE244323.1 EST_HUMAN TCBAP2E0383 Pediatric pre-B cell acute lymphoblastic leukernia Bayfor-HGSC pro.lect=TCBA Homo sapiens cONA done TCBAP0383 t50007 10109 15179 2.8 5.0012-89 BE244323.1 EST_HUMAN TCBAP2E0383 Pedlatric proB'cell acute lymphoblastic leukernia Bayfor-HGSC project=TCBA Homo saplens cIDNA done TCBAP0383 Page 139 of 196 Table 4 do A 2847 7998 13095 1,6 3.OOE-89 AW976181.1 EST-HUMAN EST388290 MAGE resequences, MAGN Horno sapiens cDNA 121 5570 10707 0,7 2.0012-89 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 121 5570 10708 0.7 2.OOE-89 7706670 NT Homo saplens PXR2b protein (PXR2b), mRNA 402 5570 10707 0.9 2.OOE-89 7706670 NT Homo sapiens KR2b protein (PXR2b), mRNA 402 5570 10708 0.9 2.OOE-89 7706670 NT Homo sapiens KR2b protein (PXR2b), mRNA 519 5683 10804 0.8 2.OOE-89 AB037763.1 NT HomosapiensmRNAforKlAA1342p toin,pardaicds 1778 6899 12086 1.3 2.OOE-89 4503314 NT Homo sapiens diacyigjycerol kinase, gamma (90kID) (DGKG) mRNA 1778 6899 12087 1.3 2.OOE-89, 4503314 NT Homo sapiens diacylglycerol kinase, gamma (9OkD) QGKG) mRNA 2852 8003 13101 2,0 2.OOE-89 A1222095.1 EST HUMAN qg96c08.xl Soares - NFL, GBC - S1 Homo sapiens cDNA done IMAGE: 1843022 X similar to gb:J04131 GAMMA-GLUTAMYLTRANSPEPTIDASE 1 PRECURSOR (HUMAN);oontains Alu repetitive element; 4053 9180 14263 1.5 2.OOE-89 AF089897.1 NT Homo sapiens topoisornerase- related function protein (TRF4-2) mIRNA, partial cds 4061 9188 14272 6.2 2.OOE-89 X58742.1 NT H.sapiens HCK gene fortyrosIne kinase (M), exons 10-11 4061 9188 14273 6.2 2.OOE-89 X58742.11 NT H.saplens HCK gene for rosine kinase (M), exons 10-11 4396 9518 14608 1.1 2.OOE-89 AJ007378.1 NT Homo saplens GGT gene, exon 5 372 5548 10681 1.1 1.OOE-89 A1652927.1 EST HUMAN wb40h06.xl NCI-CGAP_GC6 Homo sapiens cDNA done IMAGE:2308187 Ysimilar to SW:BAC1 HUMAN 014867 TRANSCRIPTION REGULATOR PROTEIN BACH1 1047 6187 11338 0.6 8.OOE-90 AL163246.2 NT Homo sapiens chromosome 21 segment HS21 C046 1048 6187 11338 1.0 8.OOE-90 AL163246,2 NT Homo sapiens_chromosome 21 segment HS21C046 818 5968 0.9 7.OOE-90 AF223391.1. NT Homo saplens calcium channel alphal E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively spliced 3029 8181 13281 1.1 6.OOE-90 X91926.1 NT H.sapiens ECE-1 gene (exon 6) 3029 8181 13282 1.1 6.OOE-90 X911926.1 NT H.sapiens ECE-1 gene (exon 6) 4134 9260 14341 7,3 6.OOE-90 8922398 NT Horno sapiens hypothetical protein FU10388 (FLJ10388), mRNA 4134 9260 14342 7.3 6.OOE-90 8922398 NT Homo sapiens hypothetical protein FLJ10388 (F1-110388), mRNA 149 5345 3.6 5.00IE-90 AB035344.1 NT Homo sapiensTCL6 gene, exon 1-10b - 1175 6309 11463 0.5 5.OOE-90 U80226.1 NT Human gamma-aminobutyric add transaminase mRNA, partial cds 2508 7604 12805 0.8 5.OOE-90 AF114487.1 NT Horno saplens intersectin long lsoform (ITSN) mRNA, complete eds 4440 9560 14656 3.1 5.OOE-90 45 6354 NT Homo saplens pregnancy-zone protein (PZP) mRNA 4507 9626 14718 1.1 5.OOE-90 AA705222.1 EST-HUMAN 7J82gl 0.s11 Soaresfetal-liver-spicen-1 WLS-S1 Homo saplens cDNA done IMAGE:461442 3 4507 9626 14719 1.1 5.OOE-90 AA705222.1 EST-HUMAN zj82g10.si Soaresfetal_iivei_spicen_1NFLS-S1 Homo sapiens cIDNA clone IMAGE:461442 3' 4571 9689 14783 1.0 5.OOE-90 AL135549.1 EST-HUMAN DKFZp762P1 616-rl 762 (synonym: hme12) Homo saplens cIDNA done DKFZp762P1 616 5, 299 5485 10615 0.6 4.OOE-90 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 299 5485 10616 0.6 4.OOE-90 AF231920.1 NT Horno saplens chromosome 21 unknown mRNA 587 5747 10864 0.6 4.OOE-90 A1905559.1 EST-HUMAN CM-BT092-0302%-022 BT092 Horno sapiens cDNA 1070 6208 11361 1,0 4.OOE-90 4505316 NT Homo sapiens myosin phosphatase, target subunit 1 (MYPT1), mRNA 1663 6787 11964 3.3 4.OOE-90 X99033.1 NT H.sapiens gene encoding discoidin receptor tyrosine kinase, exon 16 455 9675 14767 4.1 4.OOE-90 D87675.1 NT Homo sapiens DNA for amAold precursor protein, complete cds 1 Page 140 of 196 Table 4

C4 4691 9803 14904 2.0 4.OOE-90 A13033070.1 NT Homo saplens mRNA for KIAA1244 protein, partial cds 4713 9825 14917 1.9 4.OOE-90 M95967.1 NT Human prohormone converting enzyme (NEC2) gene, exon 8 718 5872 0.6 3.OOE-90 W281189.1 EST HUMAN 43c5 Human retina cIDNA randomly primed sublibrary Homo sapiens cDNA 1156 6291 11445 1.2 2.OOE-90 5031748 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 17 (HMG17), mRNA 1156 6291 11446 1.2 2.OOE-90 5031748 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 17 (HIVIG1 7), mRNA 3771 8908 13997 2.8 2.OOE-90 A1138213.1 EST-HUMAN qc54c02.xl Soares_placenta-8togweeks-2NbHP8togW Homo sapiens cIDNA clone IMAGE:1713410 3'stmilar to SMOLFR.MOUSE P23275 OLFACTORY RECEPTOR OR3.; 4588 9702 14796 1,1 2.OOE-90 A13006627.1 NT Homo sapiens mRNA for KIAA0289 gene, partial cds 4820 9929 15020 8.3 2.OOE-90 5729855 NT Homo sapiens GRI32-related adaptor protein (GRAP) mRNA 275 5463 10592 1.2 1.OOE-90 4502166 NT Homo saplens amyioid beta (A4) precursor protein (protease nexin-l I, Alzheimer disease) (APP). mRNA 370 7861 10680 1.2 1.0OE-90 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 371 7861 10680 1.0 1.OOE-90 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 680 5835 10967 0.7 1.0OE-90 AJ237589.1 NT Homo sapiens mRNA for T-box transcription factor (T13X20 gene), partial 680 5835 10968 0.7 1.05E-90 AJ237589.1 NT Homo sapiens mRNA for T-box transcription factor (T13X20 gene), partial 713 5867 11004 2.6 1.OOE-90 AF264750.1 NT Homo sapiens AIR-like protein mRNA, partial cds 713 5867 11005 2.6 1.OOE-90 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial ods 1094 6232 0.8 1.OOE-90 4507828 NT Homo sapiens Kruppet-like factor 7 (ubiquitous) (K1F7), mRNA 1284 6414 11576 1.2 1,OOE-90 AF096154.1 NT Homo saplens protein phosphatase 2A BR gamma subunit gene, exon 3 1284 6414 11577 1.2 1.00IE-90 AF096154.1 NT Horno sapiens protein phosphatase 2A BR gamma subunit gene, exon 3 1644 6770 4,0 1.0OE-90 BE379884.1 EST HUMAN 601159563F2 N1H MGC 53 Horno, sapiens cDNA done IMAGE:3511118 S' 2823 7974 13077 8.4 11.0OE-90 6005720 NT Homo sapiens chromosome 8 open reading frame 2 (C8ORF2), mRNA 3777 8914 14005 1.2 1.00IE-90 A13020710.1 NT Homo sapiens rnRNA for KIAA0903 protein, partial cds 3777 8914 14006 1.2 1.OOE-90 A13020710.1 NT Horno saplens mRNA for KIAAO03 protein, partial cds 4326 9449 14537 1.0 1.OOE-90 AF167340.1 NT Homo saplens soluble Interieukin 1 receptor accessory protein (11-1 RAP) gene, exon 8, alternative exons 9 and complete cds, alternatively spliced 4101 9227 14312 5.5 8.OOE-91 D12234.1 ESTHUMAN HUM000S381 Liver HepG2 cell line. Homo saplens cDNA clone s381 3, 1428 6555 11731 0.9 7.OOE-91 AF053768.11 NT Rattus norvegicus brain specific cortactin-binding protein CBP90 mRNA, partial cds 151 5347 10475 0.8 6.OOE-91 AW961150.1 ES-r_HUMAN EST373222 MAGE resequences, MAGF Homo sapiens cDNA 3429 8571 13683 1,5 5.OOE-91 AA702794.1 ESTHUMAN z190b04.sl Soares_fetalliver-spicerLl NFI-S-S1 Homo sapiens cDNA done IMAGE:448015 3' 3601 8740 13834 6.0 5.OOE-91 BE074985.1 EST-HUMAN PM3-BT0583-170200-001- e06 ST0583 Homo saplens cIDNA 3601 8740 13835 6.0 5.OOE-91 SE074985.1 EST-HUMAN PM3-BT0583-170200-001- e06 BT0583 Homo sapiens cDNA 4703 9815 14911 0.8 5.OOE-91 7110634 NT Horno saplens chromosome 22 open reading frame 5 (C220RF5), mRNA 4703 951b 14U1Z 0.8 5.0012-91 7110634 NT Homo saplens chromosome 22 open reading frame 5 (C22ORFS), mRNA 1223 6353 11517 2.5 4.00IE-91 BE259124.1 EST-HUMAN 601115508F1 N1H MGC 16 Homo sapiens cIDNA done IMAGE:3356376 5' 8315 13427 1.3 4.00IE-91 AF156776.1 NT Horno sapiens lysophosphafidic add acyltransferase-delta (LPAAT-delta) mRNA, complIC cds 1) Page 141 of 196 1 Table 4 .i I:I/) C.

3166 8315 13428 1.3 4.OOE-91 AF156776.1 NT Homo sapiens lysophosphatidic acid acyltransferase-delta (LPAAT-delta) mRNA, complete cds 2624 7714 12914 1.0 3.OOE-91 7706508 NT Homo sapiens beta- ureldoproplonase (LOC51733), mRNA 2624 7714 12915 1.0 3.0OE-91 7706508 NT H6mo sapiens beta- ureidoproplonase (LOC51733), mRNA 3297 8443 13552 1.8 3.OOE-91 AL163283.2 NT Homo sapiens chromosome 21 segment HS21 C083 3416 8558 13668 3.0 3.OOE-91 AB033104.1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 3416 8558 13669 3.0 3.0OE-911 AB033104.1 NT Homo saplens mRNA for KIAA1278 protein, partial cds 3720 8U57 13950 0.8 3.OOE-91 AF084530.1 NT Homo sapiens cydin-D binding MybAlke protein mRNA, complete eds 4487 9607 14700 4.0 3.OOE-91 M30938.1 NT Human Ku (p70/p80) subuffit mRNA, complete cds 4905 10012 15092 1.2 3.OOE-91 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 4905 10012 15093 1.2 3.OOE-91 AL163285.2 NT Horno sapiens chromosome 21 segment HS21 C085 1699 7924 12004 0.9 2.OOE-91 AW845499.1 EST-HUMAN MR1-CTO056-241199-009- b07 CTO056 Homo saplens cONA 42 5252 10365 1.0 1.OOE-91 AL1 63284,2 NT Homo sapiens chromosome 21 segment HS21 C084 1225 6355 11519 1.2 1.OOE-91 AW449746.1 EST-HUMAN Ul-H-B13-aks-d-01-0-Ul. sl NCLCGAf_Sub5 Homo sapiens cDNA done IMAGE:2735280 3' 435 7888 10733 0.8 9.0012-92 AW858856.1 EST-HUMAN IRC3-CTO347-220300-015- g12 CTO347 Homo saplens cDNA 435 7888 10734 0.8 9.OOE-92 AW858856.1 EST_HUMAN IRC3-CTO347-220300-015- g12 CTO347 Homo sapiens cDNA 1221 6352 11513 2.9 9.OOE-92 AJ001689.1 NT Homo sapiens NKG2D gene, exon 10 1221 6352 11514 2.9 9.OOE-92 A.1001689.1 NT Homo sapjens NKG2D gene, exon 10 87 5293 10419 0.7 8.OOE-92 W26367.1 EST-HUMAN 20 Human retina cDNA randomly primed;ublibrary Homo sapiens cDNA 283 5470 10600 2.0 8.OOE-92 BE386363.1 ESI_HUMAN 601273513F1 N1H MGQ_20 Homo sapiens cDNA done IMAGE:3614667 5' 2738 7822 13026 0.9 8.OOE-92 BE396819.1 EST HUMAN 601290085F1 N1H MGC 8 Homo saplens cIDNA. clone IMAGE:3620469 5 5268. 10390 0.9 7.OOE-92 M60676.1 NT Human von Willebrand factor pseudogene corresponding to exons 23 through 34 236 7884 10552 0.9 7.OOE-92 A8018301.1 NT Homo sapiens mIRNA for KIAA0758 protein, partial cds 236 7884 10553 0.9 7.OOE-92 AB018301.1 NT Homo sapiens mRNA for KIAA0758 protein, partial ods 576 5736 1.3 7.OOE-92 AF007822.1 NT Homo sapiens cytoplasmic Seprase truncated i;oform mRNA, complete cds 1259 6388 11.549 0.7 7.OOE-92 4502384 NT Homo sapiens 13-cell CLLAymphoma 7b (BCL7B) mRNA 2139 7240 12455 0.6 7.OOE-92 5031570 NT Homo sapiens ARP2 (actin-related protein 2, yeast) homolog (ACTR2), rnRNA 2139 7249 12456 0,6 7.OOE-92 5031570 NT Homo sapiens ARP2 (adin-related protein 2, yeast) homolog (ACTR2), mRNA 2517 7613 12815 0.8 7.OOE-92 AF167706.1 NT Horno sapient cystelne-rich repeat-containing protein S5 precursor, mRNA, complete cds 2693 7780 12977 1.8 TOCIE-92 6005738 NT Horno sapiens NRAS-related gene (D1S155IE), mRNA 2724 7808 13013 1.0 7.OOE-92 A8031007.1 NT Homo sapiens DNA, WC class 1 region, 7.1 ancestral haRlotype 3301 10296 13555 0.9 7.OOE-92 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 3301 10296 13556 0.9 7.OOE-92 4507500 NT Homo sapiens T-cell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 4484 9604 14697 1.6 7.OOE-92 S71824.1 NT N-CAM=145 kda neural call adhesion molecule fhuman, small call lung cancer cell line OS2 R, mRNA, 2960 nt] 4484 9604 14698 1.6 7.OOE-92 S71824.1 NT N-CAM=1145 kda neural cell adhesion molecule [human, small cell lung cancer cell line OS2 R, mRNA, 2960 nt) 5110 10206 15284 1,5 7.OOE-92 4506118 NT Homo sapiens prospero-related homeobox 1 (PROX1) mRNA 66951:::

15691 0,9 5.0012-92 BE390882.1 EST HUMAN 601283012F1 N1H MGQ_44 Homo saplens cDNA clone IMAGE:3605018 5' 1 Page 142 of 196 Table 4

A C11A C 23 5232 10335 0.5 2.0012-92 4501898 NT Homo sapiens activin A receptor, type IIB (ACVR2B) mRNA 732 5888 11029 1.4 2.OOE-92 BE299190.1 EST-HUMAN 601118337F1 N1H.10GQ_17 Homo sapiens cDNA clone IMAGE:3028304 5, 732 5886 11030 1.4 2.OOE-92 GE299190.1 EST HUMAN 601118337F1 NIH MGC 17 Homo sapiens cDNA done IMAGE:3028304 5, 1686 6810 1.0 2.OOE-92 S78653.1 NT mrg=mas-related [human, Genomic, 2416 nt] 1894 7011 12199 0.6 2.OOE-92 A1818119.1 EST HUMAN wk27d07.xl NCLWAP-13m25 Homo sapiens cDNA done IMAGE:2413549 3'similar to TR:Q12844 Q12844 BREAKPOINT CLUSTER REGION PROTEIN; 1894 7011 12200 0.6 2.OOE-92 A18181 19,1 EST HUMAN wk27d07.xl NCI__CGAP_Bm25 Homo sapiens cIDNA done IMAGE:2413549 Ysimilar to TR:Q12844 Q12844 BREAKPOINT CLUSTER REGION PROTEIN; 2002 7117 12319 1.7 2.OOE-92 4506860 NT Homo saplens syndecan 4 (amphiglycan, ryudocan) (SDC4) mRNA 2623 7713 12913 14.0 2.OOE-92 6912457 NT Homo saplens caldneurin binding protein 1 (K1AA0330), mRNA 3562 8701 13797 1.0 2.OOE-92 AF231919.1 NT Homo sapiens chrorhosome 21 unknown mRNA 3562 8701 13798 1,0 2.OOE-92 AF231919.1 NT Homo sapiens chromosome 21 unknown mIRNA 3631 8770 13864 5.0 2.OOE-92 5803180 NT Homo sapiens stress-induced- phosphoprotein 1 (Hsp70IHspgO-organizlng protein) (STIP1), mRNA 1 4191 '9317 14401 1.0 2.OOE-92 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 4648 9760 14855 0.8 2.OOE-92 AF136523.1 NT Homo saplens bile salt export pump (BSEP) mRNA, complete cds 4922 10029 2.5 2.OOE-92 A1.040437.1 EST HUMAN DKFZp434C0414-rl 434 (synonym: htes3) Homo sapiens cDNA clone DKFZp434C0414 5, 1807 6926 12114 1.1 1.0OE-92 R78078.1 EST_HUMAN y180e08.rl Scares placenta NWHP Homo saplens cDNA done IMAGE:14557.4 Y 1807 6926 12115 1.1 1.OOE-92 R78078.1 EST HUMAN y180e08.rl Scares placenta Nb21-1P Homo sapiens cIDNA done IMAGE:145574 V 2028 7141 12342 13.0 1.0OE-92 4506668 NT Homo sapiens dbosornal protein. large, Pl.(RPI-131) mRNA 1996 7112 3.3 9.OOE-93 AA316723.1 EST-HUMAN EST188414 HCC call line (matastasis to liver In mouse) 11 Homo saplens cIDNA Yend similar to dbosomal protein L29 2610 7700 0.5 9.OOE-93 AF223391.1 NT Homo sapiens calcium channel alphal E subunit (CACNAI E) gene, exons 7-49, and partial ods, altemafivelyspliced 3563 8702 13799 1.0 9.OOE-93 BE3885711.1 EST-HUMAN 601281867F1 N1H MGC 44 Horno sapiens cDNA done IMAGE:3603832 5' 246 5434 10560 2.1 7.OOE-93 AF231919.1 NT Homo sapiens chromosome 21 unknown mRNA 3030 8182 2.4 7.OOE-93 AL1 63247,2 NT Homo sapiens chromosome 21 segment HS21C047 1359 6487 11653 1.3 5.OOE-93 AB014511.1 NT Homo saplens mRNA for KIAA0611 protein, partial cds 1385 6512 11677 2.0 5.OOE-93 A1674184.1 EST HUMAN wcO9cO8.xl NCI_WAP Pr28 Homo sapiens cIDNA. done IMAGE:2314670 3' 1385 6512 11678 2.0 5.OOE-93 A1674184.1 EST HUMAN wc09c08M NCI CGAF_Pr28 Homo sapiens cDNA done iMAGE:2314670 3, 1459 6586 1.0 5.OOE-93 AL163201.2 NT Homo saplens chromosome 21 segment HS21 C001 3195 8344 13457 2.4 5.OOE-93 X042011.1 NT Human skeletal muscle 1.3 kb mRNA for tropomyosin 82 5288 1.6 4.OOE-93 AA459933.1 EST HUMAN zx50e09.si Soares-tesds-NHT Homo saplens cDNA done IMAGE:795688 Ysimilar to SW:CLPA-RAT P37397 CALPONIN, ACIPIC ISOFORM; 437 5603 10736 0.6 4.OOE-93 4557879 NT Homo sapiens Interferon gamma receptor 1 (IFNGRI) mRNA 437 5603 10737 0.6 4.OOE-93 4557879 NT Horno sapiens Interferon gamma receptor 1 (IFNGR1) mRNA 755 5907 11054 1.3 4.OOE-93 7657454 NT Homo saplens poscadillo (zebrafish) homolog 1, containing BIRCT domain (PES1), mRNA 755 5907 11055 1,3 4.OOE-93 7657454 NT Homo sapiens pescadillo (zebrafish) homolog 1, containing BIRCT domain (PES1), mRNA U Page 143 of 196 Table 4

J_ 4 1166 6300 11455 0.7 4.OOE-93 8923658 NT Homo sapiens hypothetical protein FLJ20731 (FL.120731), mRNA 1934 7051 12244 1.8 4.OOE-93 AF047677.1 NT Homo sapiens dystrophin (DMD) gene, deletion breakpoints 1-3 in intron 5 2349 7451 12658 0.8 4.OOE-93 AL163301.2 NT Homo sapiens chromosome 21 segment HS21 Cl 01 2564 7657 12860 0.8 4.OOE-93 7656972 NT Homo sapiens TNF4ndudble protein CG12-1 (CG12-1), mRNA 3962 9094 14179 1.4 4.OOE-93 4504654 NT Homo sapiens Interleukin 18 receptor 1 (11-18R1) mRNA 183 5377 10502 2.8 2.OOE-93 AB015610.1 NT Chlorocebus aethiops mRNA for dbosomal protein S4X, complete cds 183 5377 10503 2.8 2.OOE-93 AB015610.1 NT Chlorocebus aethiops mRNA for ribosomal Crotein S4X, complete eds 320 5504 10632 2.3 2.OOE-93 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 321 5504 10632 2.6 2.OOE-93 AL163285.2 NT Homo sapiens chromosome 21 segment HS21C085 2083 7195 12400 1.0 2.OOE-93 4758105 NT Homo sapiens Clk-assoclating RS- pydophilin (CYP) mRNA 2436 7535 12742 0.9 2.OOE-93 BE253201.1 EST-HUMAN 601116810F1 N1H MGC 16 Homo saplens cDNA done IMAGE:3357243 5' 5088 10186 15264 1.0 2.OOE-93 BE253201.1 EST HUMAN 601116810F1 N1H MGC 16 Homo saplens cDNA done IMAGE:3357243 97 96 5303 10429 0.6 1.OOE-93 AF238997.1 NT Homo sapiens CTR1 pseudogene 96 5303 10430 0.6 1.0OE-93 AF238997.1 NT Homo sapiens CTR1 pseudogene 507 5671 10794 0,9 1.0OE-93 7657016 NT Homo saplens hypothetical protein (DJ328E19,Cl.1), mRNA 585 5745 10862 1.3 1.OOE-93 A1146755.1 EST-HUMAN oy84b08.xl NCI-CGAP-CLI- 1 Horno sapiens eDNA clone IMAGE: 1672503 Ysimilar to TR:Q62384 Q62384 ZINC FINGER PROTEIN.; 854 6003 11163 1.1 1.OOE-93 D87675.1 NT Homo sapiens DNA for amyioid precursor protein, complete cds 1217 6347 11506 2.2 1.0012-93 8923270 NT Homo sapiens hypothetical protein FLJ20291 (FL.120291), mRNA 1217 6347 11507 2.2 1.OOE-93 8923270 NT Homo sapjens hypothetical protein FLJ20291 (FI-J20291), mRNA 1323 6452 11617 0.6 1.0OE-93 AF167706.1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 2289 7394 12602 1.0 1.OOE-93 AF231981.1 NT Homo sapiens long chain polyunsaturated fatty acid elongation enzyme (HEL01) mRNA, complete cds 2415 7514 12722 1,5 11.0OE-93 AF055066.1 NT Horno sapiens MI-IC class 1 region 2459 7558 1.1 1.0012-93 A8037846.1 NT Homo sapiens mRNA for KIAA1425 protein, partial cds 2792 6403 11562 0.9 1.OOE-93 BE297369.1 EST HUMAN 601177686F1 NIH MGC 17 Homo saplens cDNA clone IMAGE:3532965 5' 2792 6403 11563 0.9 1.OOE-93 BE297369.1 EST HUMAN 601177686F1 N1H MGC 17 Homo sapiens cDNA done IMAGE:3532965 9 2903 8054 13159 4.3 1.OOE-93 D87675.1 NT Homo sapiens DNA for arnylold precursor protein, complete cds 4331 9454 14542 1.4 1.OOE-93 AL163284.2 NT Horno saplens chromosome 21 segment HS21 C084 3880 9016 14111 1,7 6.OOE-94 AF142482.1 NT Homo saplens transcription enhancer factor-5 mRNA, complete cds 1799 6919 1.7 4.OOE-94 1.05094.1 NT Homo sapiens dbosomal protein L27 mRNA, complete ods 2621 7711 12911 0.9 4.OOE-94 4506008 NT Homo sapiens protein phosphatase 1, regulatory subunit 10 (PPPIR10) mRNA 4618 9732 14827 3,0 4.OOE-94 A1591312.1 EST-HUMAN twl 1fl 0.xl NCLWAP- 13m52 Homo saplens cDNA done IMAGE:2259403 Ysimilar to TR:C115265 Q15265 PROTEIN TYROSINE PHOSPHATASE; 595 5753 10871 1,2 3.OOE-94 AB022785.1 NT Ho mo sapiens ASH2L gene, complete cds, similar to Drosophila ash2 gene 704 5859 10994 1.2 3.00ES4 4502506 NT Horno, saplens complement component 5 (C5) mRNA 1706 6829 12009 1.1 3.OOE-94 AF167706.1 NT Homo saplens cystelne-rich repeat-containing protein S52 precursor, mRNA, complete cds 1 6829 12010 1.1 3.OOE-94 AF167706.1 NT Homo sapiens eysteine-dch repeat- containing protein S52 precursor, mRNA, complete cds Page 144 of 196 Table 4

1735 6858 12036 1.3 3.OOE-94 4557556 NT Horno sapiens E1 A binding protein p300 (E1R300) mRNA 4695 9221 14304 0.8 3.OOE-94 AA464805.1 EST HUMAN zw63908.rl Soares-totai- fetu5_Nb2HF8-gw Homo sapiens cIDNA done IMAGE:774782 5, 143 5,339 10469 0.8 11.0OE-94 BE295714.1 EST-HUMAN 601175762F1 Nib_1VIGC 17 Horno sapiens cDNA done IMAGE:3531038 5' 616 5774 10895 1.5 1.OOE-94 A1394483.1 EST-HUMAN tf79f03.xl NCLWAP-8m23 Homo sapiens cDNA done IMAGE:2105501 Ysimilar to TR:008764 008764 ANKYRIN.; 616 5774 10896 1.5 1.OOE-94 A1394483.11 EST HUMAN tf79f03.xl NCI_PGAP_Bm23 Horno sapiens cDNA done IMAGE:2105501 Ysimilar to TR:008764 008764 ANKYRIN.:

3050 8201 13301 1.9 1.0012-94 BE253433.1 EST HUMAN 601111696F1 NIH MGC 16 Homo sapiens c(DNA done IMAGE:3352559 5' 3050 8201 13302 1.9 1.OOE-94 BE253433.1 EST-HUMAN 601111696F1 NIH MGC 16 Horno, sapiens cDNA done IMAGE:3352559 5' 1461 6588, 11762 0.6 9.OOE-95 AF027302.11 NT Homo sapiens TNF-alpha stimulated ABC protein (ABC50) mIRN, complete cds 3118 8267 13371 1.1 9.OOE-95 7662027 NT Horno sapiens KIAA0255 gene produ (K1AA0255), mRNA 3118 8267 13372 1.1 9.OOE-95 7662027 NT Horno sapiens KIAA0255 gene product (K1AA0255), mRNA 4436 9557 14651 1.6 8.OOE-95 A1700998.1 EST-HUMAN weO9eO4.xl NCI_CGAF_Lu24 Horno sapiens eDNA done IMAGE:2340606 Ysimilar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN); 4436 9557 14652 1.6 8.OOE-95 A1700998.1 EST-HUMAN we09c04.xl NCI_WAP-Lu24 Homo sapiens cDNA done IMAGE:2340606 Ysimilar to gb:1(00558 TUBULIN ALPHA-1 CHAIN (HUMAN); 274 5462 10590 3.2 7.OOE-95 D87675,1 NT Homo sapiens DNA for amylold precursor p in. complete cds 274 5462 10591 3.2 7.OOE-95 D87675.1 NT Homo sapiens DNA for amytold precursor rotein, complete ccis 4270 9395 14479 5.9 7.OOE-95 M95708.1 NT Horno sapiens Ly-6-like protelin (C1)59) mRNA, complete ods 4316 9439 1.4 7.OOE-95 AL-163246.2 NT Horno sapiens chromosome 21 segment HS21 C046 4982 10085 15155 1,0 7.OOE-95 M95929.1 NT Hurnan homeobox protoinAPHOXI) mRNA, Yend 922 6069 11226 0.9 2.0012-95 4504374 NT Homo sapiens H factor 1 (complement) (HF1) mRNA 1625 6751 11919 0.6 2.OOE-95 7662027 NT Homo sapi s KIAA0255 gene product (K1AA0255), mRNA 1625 6751 11920 0.6 2.OOE-95 7662027 NT Horno sapiens KIAA0255 gene product (K1AA0255), mRNA 1897 7015 12204 2.8 2.OOE-95 4507512 NT Horno sapiens tissue Inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory) (TIMP3) mRNA 1900 7018 12208 1.2 2.OOE-95 BE393873.1 EST HUMAN 601312161 F1 N11- 1_1v1GC 44 Horno sapiens cDNA done IMAGE:3658862 5, 2376 7478 12679 1.3 2.OOE-95 5453665 NT Horno sapiens G protein-coupied receptor 19 GPIR19) mRNA 2376 7478 12680 1.3 2.OOE-95 5453665 NT Homo sapiens G protein-coupled receptor 19 (GPR19) mRNA 2417 7516 12723 16.6 2.OOE-95 7657185 NT Homo sapiens hypothetical p tein (HS322BIA), mRNA 2466 7564 W67 0.9 2.OOE-95 4758423 NT Homo sapiens glydne deav6ge system protein H ( minomethyl carder) (GCSH) mRNA 2787 6068 11225 0.9 2.0OE-95 4504374 NT Horno sapiens H factor 1 (complement) (HIF1) mRNA 3120 8269 13374 3.5 2.OOE-95 AF015452.1 NT Horno sapie s Usurpin-gamma mRNA, complete cds 3517 8658 13764 2.8 2.OOE-95 7705900 NT Horno sapie s unconventional myosin-1 5 ( 0C51168), mRNA 3517 8658 13765 2.8 2.OOE-95 7705900 NT Homo sapiens unconventional myosin-15 (LOC51168), mRNA 3565 8704 13800 1.0 2.OOE-95 AB037807.1 NT Horno sapiens mRNA for KIAA1386 protein, partial cds 3690 8829 13922 1.0 2.OOE-95 A1290264.1 EST-HUMAN cim01c02.xl SoaresLNhi- IMPuSI Homo sapiens cDNA done IMAGE:1 880546 Ysimilar to WRT23G7.4 CE03705; 4264 9389. 14472 2.3 2.OOE-95 7657185 NT Homo sapiens hypothetical protein (HS322BIA), mRNA ,10074 15147 2.6 2.OOE-95 7661979 NT Homo sapiens KIAA0187 gene product (K1AA:0 87), mRNA Page 145 of 196 1 Table 4

5022 10123 15195 1.0 2.OOE-95 AA447931.1 EST HUMAN zxl 1 d07.rl Soarestotal-fetus_Nb2HF8-gw Homo saplens cIDNA clone IMAGE:786157 5' 5022 10123 15196 1.0 2.OOE-95 AA447931.1 EST-HUMAN zxl 1d07.rl Soarestotai-fetus-Nb2HF8-gw Homo sapiens cDNA clone iMAGE:786157 5, 1693 6817 11997 0.8 8.OOE-96 7657185 NT Homo sapiens hypothetical protein (HS322B1A), mRNA 1693 6817 11998 0.8 8.OOE-96 7657185 NT Homo sapiens hypothetical protein (HS32213IIA), mRNA.

3834 8971 14070 1.0 7.OOE-96 AF2311920.1 NT Homo sapiens chromosome 21 unknown mIRNA.

4450 9570 14668 2.0 7.OOE-96 AW404800.1 EST HUMAN Ul-HIF-131-0-acm-d-11 10-Ul.rl N11-1,_MGQ_37 Horno sapiens cIDNA clone IMAGE:3059397 V 4450 9570 14669 2.0 7.OOE-96 AW404800.1 EST-HUMAN Ul-HIF-BLO-acm-d-1 1-0- Ul.rl NIH-MGC-37 Homo sapiens cIDNA clone IMAGE:3059397 5' 2213 7321 12535 0.9 6.OOE-96 BE171984.1 EST-HUMAN MRO-HT0559-250200-002- d07 HT0559 Homc sapiens cDNA 3276 8421 13529 1.0 6.OOE-96 AL1 63201.2 NT Homo saplens chromosome 21 segment HS21C001 3437 8579 13691 26.2 6.OOE-96 M26873.1 NT Human glyceraidehyde-3- phosphate dehydrogenase pseudogene Yend 317 5501 10628 0,9 5.OOE-96 AB032998.1 NT Homo sapiens mRNA for K1AA1 172 protein, partial cds 824 5973 1.1131 1.0 5.OOE-96 A13032998.1 NT Homo sapiens mRNA for K1AA1 172 protein, partial cds 824 5973 11132 1.0 5.OOE-96 A13032998.1 NT Homo sapiens mRNA for K1AA11 172 protein, partial ods 2991 14 13245 1.0 5.OOE-96 6912735 NT Homo saplens transient receptor potential channel 5 (TRPCS), mRNA 8143 8 3 4810 20 1.2 5.OOE-96 X608112.1 NT H.sapiens DNA for monoamine oxidase type A (7) (partial) 9920 4097 9 6.2 3.OOE-96 H68656.1 EST-HUMAN yr87hl 2.rl Scares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE:212327 5' 2 2 9223 3 409 5577 1.2 2.OOE-96 4503098 NT Homo sapiens chondroitin sulfate proteoglycan 4 (melanorna-assodated) (CS1PG4), mIRNA 51884 730 11026 0.5 2.OOE-96 ALI 63248.2 NT Homo sapiens chromosome 21 segment HS21 C048 4651 9763 14858 1,6 2.OOE-96 BE148074.1 EST-HUMAN RC3-HT0230-040500-110- 902 HT0230 Homo sapiens cDNA 655 512 10940 0.6 11.0OE-96 Y18890.1 NT Human endogenous retrovirus type K (HERV-K), gag, pol and env genes 8 1742 6865 12042 0.7 1.OOE-96 AW955054.1 ESTHUMAN EST367124 MAGE resequences, MAGC Horno sapiens cDNA 1742 6865 12043 0.7 1.00Er96 AW9550.54.1 EST-HUMAN EST367124 MAGE resequences, MAGC Homo saplens cDNA 1806 6925 12112 0.9 1.OOE-96 4503756 NT Homo saplens flavin containing monooxygenase 2 (FIVI02) mRNA 1806 6925 12113 0.9 1.OOE-96 4503756 NT Horno sapiens flavin containing monooxygenase 2 (FIVI02) mRNA 2080 7192 12395 0.9 1.0012-96 AF167706.1 NT Homo sapiens cystelne-rich repeat-containing protein S5 precursor, mRNA, complete eds 2080 7192 12396 0.9 1,OOE-96 AF1 67706.1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 21 L31 7250- 12501 1,3 1.OOE-96 M75967.11 NT Human hepatocyte growth factor gene, exon 1 2181 1290 12502 1.3 11.0OE-96 M75967.11 NT Human hepatocyte growth factor gene, exon 1 2219 7872 12541 0.7 1.OOE-96 U511472.2 NT Felis catus superfast myosin heavy chain (sMyHC) mRNA, complete cds 924 6071 11229 1.3 4.OOE-97 BE004436.1 EST---HUMAN CMO-BN0106-170300-293- a06 BN0106 Homo sapiens cIDNA 1868 6986 12170 1.1 4.OOE-97 5453572 NT Homo sapiens brefoldin Ainhibited quanine nucleofide-exchange protein 2 (BIG2), mRNA ldlfu 5432 1.2 3.0012-97 A13032998.1 NT Homo sapiens mIRNA for K1AA1 172 protein, partial cds Page 146 of 196 Table 4

520 5 684 10805 1.1 3.OOE-97 AA042813.1 EST HUMAN zk53c07.s11 Soares_pregnant-uterus-NbHPU Horno sapiens cIDNA clone IMAGE:486540 3' similar to qb:X65857 - edsl OLFACTORY RECEPTOR-LIKE PROTEIN HG1VIP0712 (HUMAN); 856 6005 11165 3.7 3.OOE-97 4502166 NT Homo sapiens amytoid beta (A4) precursor protein (protease nexin-li, Aizheimer disease) (APP), ffiRNA 856 6005 11166 3.7 3.OOE-97 4502166 NT Homo saplens arnylold beta (A4) precursor protein (protease nexin-li, Alzhelmer disease) (APP), mRNA 1423 7916 11727 0.6 3.OOE-97 4758813 NT Homo sapiens N-myc (and STAT) Interactor (NMI), mRNA 2389 7874 12693 0.7 3.OOE-97 U36255.11 NT Human beta-prime-adapfin (BAM22) gene, exon 7 3223 8371 13480 1,1 3.OOE-97 5174478 NT Horno sapiens pericentrin (PCNT) mRNA 4670 9782 14881 12.6 11.0OE-97 4503470 NT Homo sapiens eukaryofic translation elongation factor 1 alpha 1 (EEF1A1) mRNA 883 6031 11190 2.7 9.OOE-98 BE090973.1 EST-HUMAN PM4-13T0724-010400-008- a12 BT0724 Homo sapiens ciDNA 1255 6384 115,44 1.3 9.0012-98 8393092 NT Homo sapiens cat eye syndrome critical region gene 1 (CECR1), mRNA 62 5270 10391 0.5 8.OOE-98 4759329 NT Homo sapiens Tesds-specific W- related protein on Y (XKRY) mRNA 62 5270 10392 0.5 8.OOE-98 4759329 NT Horno sapiens Tests-specific W- related protein on Y (WRY) mRNA 1350 6478 11644 0.9 8.OOE-98 A13033768.1 NT Homo sapiens hPAD-wlonylO mRNA for pepddyfarginine deiminase type 1, complete cds 1540 6666 11835 1,0 8.OOE-98 5031810 NT Homo sapiens 11-2-1ndudble T-cell kinase, (ITK), mRNA 1540 6666 11836 1.0 8.OOE-98 5031810 NT Homo sapiens 11-2-1ndudble T-cell kinase (ITK), mRNA 1695 6819 12000 1.0 8.OOE-98 AB017007.1 NT Homo sapiens PMS2L16 mRNA, partial cds 1695 6819 12001 1,0 8.OOE-98 AB017007.1 NT Homo sapiens PMS2L1 6 mRNA, partial ods 3726 8863 13956 5.0 8.OOE-98 J04469.1 NT Human mitochondrial creafine kinase (CKMT) gene, complete cels 5049 10149 0.9 8.OOE-98 AL1 63201.2 NT Homo sapiens chromosome 21 segment HS21 C001 2565 7658 12861 0.7 3.OOE-98 A13014607.1 NT Homo sapiens mRNA for KIAA0707 protein, partial cds 2720 7804 0.8 3.OOE-98 AA077498.11 EST HUMAN 7B18H01 Chromosome 7 Fetal Brain cIDNA Library Homo sapiens cDNA done 7B18H01 2033 7146 12348 11.0 2.0OE-98 BE294281.11 EST HUMAN 601172658F1 NI!:LWQ_117 Homo saplens eDNA done IMAGE:3528134 5' 2191 7300 12515 0,5 2.OOE-98 A1.163202.2 NT Homo sapiens chromosome 21 segment HS21 C002 2674 7761 12961 0.7 2.OOE-98 7662405 NT Homo sapiens KIAA0957 pro In (K1AA0957), mRNA 4244 9369 14448 4.9 2.OOE-98 4758331 NT Homo sapiens fatty-add-Coenzyme A ligase, long-chain 4 (FACL4) mRNA 4720 9832 14922 1.5 2.OOE-98 AF218902.1 NT Homo saplens attractin precursor (ATRN) gene, exon 16 4720 9832 14923 1.5 2.OOE-98 AF218902.1 NT Homo sapiens attractin precursor (ATRN) gene, exon 16 5069 10167 15243 6.4 2.OOE-98 9055269 NT Homo sapiens low density 11poprotein receptor related protein-deleted In tumor (I-RPIDIT), mRNA 5069 10167 15244 6.4 2.OOE-98 9055269 NT Homo sapiens low density lipoprotein receptor related protein-deleted in tumor (LRPDIT), mRNA 5163 10257 15327 1.1 2.OOE-98 4758975 NT. Homo sapiens protein tyrosine kinase 2 beta (PTK2B) mRNA 399 5567 10704 18.9 1.OOE-98 A1862007.1 EST-HUMAN tw36b04.xl NCI_CGAP_Utl Homo sapiens cIDNA done IMAGE:2261743 Ysimilar to SW:R1.213-HUMAN P29316 60S RIBOSOMAL PROTEIN L23A.

5613 10744 0,8 11.0OE-98 AW99861 1.1 EST-HUMAN PMO-BN0065-100300-001-c06 BN0065 Homo saplens cIDNA 17 6877 12058 61 1 nniz-a yv23f05xl Soares fetal liver spleen 1INFILS Homo sapiens cDNA done IMAGE:243585 5' IRS54204 S54204 ribosomal protein L29 - human; Page 147 of 196 Table 4

1 1975 7090 12291 0.9 7.OOE-99 4759149 NT Homo saplens SIVIC (mouse) homolog, Y chromosome (SIVICY) mRNA 1975 7590 12292 0.9 7.OOE-99 4759149 NT Homo sapiens SIVIC (mouse) homolog, Y chromosome (SMCY) mRNA 3814 8951 14049 1,9 6.OOE-99 AW976364.1 EST-HUMAN EST388473 MAGE resequpnces, MAGN Homo sapiens cDNA 4642 9756 14850 1.2 6.0012-99 4502660 NT Homo sapiens CD34 antigen (C1334) mRNA 902 6049 11203 0.9 5.OOE-99 U35464.11 NT HumanRrotein C inhibitor (PCI- B) mRNA, complete cds 902 6049 11204 0,9 5.OOE-99 U3 NT Human protein C inhibitor (PC]-B) mRNA, complete cds 1922 7039 12228 0.9 5.OOE-99 Y1 1365.1 NT H.wpiens; IMPA gene, exon 8 4463 9583 14680 1.4 5.OOE-99 AF009660.1 NT Homo sapiens T cell receptor beta locus, TCR8V7S3A2 to TCRBV1 2S2 region 5066 10164 15241 2.5 5.0OE-99 4758697 NT Homo sapiens mannosidase, alpha, class 2A, member 1 (MAN2A1), mIRNA 375 5551 10684 1.0 2.OOE-99 AW385237.1 EST-HUMAN PM1-HT0454-301299-001- 904 HT0454 Homo sapiens cONA 1219 6350 1.2 2.OOE-99 AW274792.1 EST-HUMAN xpOge06.xl NCI_PGAP - HN9 Homo saplens cDNA clone IMAGE:2739874 Ysimilar to gb;M31212 MYOSIN LIGHT CHAIN ALKALI, NON-MUCLE ISOFORM (HUMAN); 3220 8368 13479 1.1 2.OOE-99 M30938.1 NT Human Ku (p701p80) subunit rnRNA, complete cds 4443 9563 14659 3,2 2.OOE-99 AF095703.1 NT Horno sapiens short chain L-3- hydroxyacyl-CoA, dehydrogenase precursor (HADHSC) gene, nuclear gene encoding mitochondrial protein, complete cds 312 5496 10625 0,5 1.0OE-99 AF1 14487.1 NT Homo sap(ens intersectin long isoform (ITSN) mRNA, complete cds 1401 6528 11698 0.8 1.0OE-99 M30938.1 NT Human Ku (p701p80) subunit mIRNA, complete ods 1537 6663 11830 0.5 1.OOE-99 AF192523.1 NT Homo sapiens truncated Niemann- Pick C3 protein (NPC3) mRNA, complete cds 1537 6663 11831 0.5 1.OOE-99 AF192523.1 NT Horno sapiens truncated Ntemann-Pick C3 protein (NPC3) mRNA, complete cds 1885 7003 12188 1,1 1.OOE-99 4503730 NT Homo sapleM FK506-binding protein 6 (36kID) (FKBP6) mRNA, and translated products 1885 7003 12189 1.1 1.OOE-99 4503730 NT Homo sapiens FK506-binding protein 6 (36k13) (FKi3P6) mRNA, and translated products 3046 8197 13298 0.9 1.OOE-99 J03171.1 NT Human Interferon-alpha receptor (HuIFN-alpha-Rec) mIRNA, complete cds 4283 9408 14492 2.7 1.OOE-99 AF098018.1 NT Horno sapiens fatty add amide hydrolase (FAAH) gene, exon 14 4283 9408 14493 2.7 1.0OE-99 AF098018.1 NT Homo sapiens fatty add amide hydrolase (FAAH) gene, exon 14 5200 10291 1.2 1.OOE-99 AL1 63281.2 NT Homo sapiens chromosome 21 segment HS21 C081 1 5211 10312 1,1 1.OOE-100 AL.163247.2 NT Homo saplens chromosome 21 segment HS21C047 2 5211 10312 0.7 1.OOE-100 AL.163247.2 NT Homo sapiens chromosome 21 segment HS21C047 81 5287 10415 0.5 1.OOE-100 AW275237.1 EST - HUMAN xv78bl 1.xl NCI_CGAP_Bm53 Homo saplens cDNA done IMAGE:2824605 3' 162 5357 10482 1.2 I.OOE-100 AL.163206.2 NT Homo sapiens chromosome 21 segment HS21 COW 314 5498 10627 1.0 1,OOE-100 AL.163249.2 NT Homo sapiens chromosome 21 segment HS21 C049 340 5521 10646 0.8 1.OOE-100 TO5087.1 EST HUMAN EST02975 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA done HFBCR32 430 5597 0.5 1.0OE-100 AF003528.1 NT Homo sapiens X-linked anhidroidc ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 481 5647 2.4 1.0OE-100 X89631.1 NT G.godlia DNA for ZNF80 gene homolog 500 5664 10787 1.4 1.0OE-100 BE180609.1 EST-HUMAN RC3-HT0625-040500-022- b09 HT0625 Homo sapiens cDNA 969 6114 11273 4.3 1.0OE-100 7705430 NT Homo sapiens hypothetical, protein (HSPC016), mRNA 1002 6142 11295 0.8 1.OOE-100 7661685 NT Homc sapiens DKFZP586M01 22 protein (DKFZP586M0122), mRNA 1002 6142 11296 0.8 1.OOE-100 7661685 NT Horno s;piens DKFZP586M0122 protein (DKFZP586M0122), mIRNA 1528 6655 1.3 1.OOE-100 AW207555.1 EST_HUMAN Ul-H-Bil-afk-c,07-0-Ul.sl ISICI-PGAP-Sub3 Homo sapiens c[DNA clone IMAGE:2722164 3' 0 Page 148 of 196 Table 4

* G- A r 1533 6659 11826 0.5 1.OOE-100 A1200857.1 EST - HUMAN qf62f09.xl Soares tesds NHT Homo sapiens cIDNA cJone IMAGE:1 754633 Ysimilar to SW:CY-T-COTJA P8106-1 CYSTATIN; 2195 7304 0.5 1.0OE-1100 D83349.1 NT Rat mRNA for short type PB-cadherin, complete cds 2294 7398 12607 1.5 1.OOE-100 AW937782.1 EST-HUMAN QV3-DT0045-140200-082- el 1 DT0045 Homo saplens cIDNA 2388 7489 12692 1.1 1.OOE-100 AF164788.1 NT Pan troglodytes Interferon gamma precursor G) mRNA, partial cds 2464 7562 12766 0.7 1.OOE-100 W26450.11 EST HUMAN 30a2 Human retina cIDNA randomly primed sublibrary Homo sapiens cDNA 2985 8137 2.5 1.!LOE-100 D1 1078.1 NT Homo sapiens RGH2 gene, retrovirus- like element 4116 9242 14328 1.5 1.OOE-100 AF057354.1 NT Homo saplens myotubuladn- related protein la mRNA, partial cds 4143 9269 14348 1.9 1.OOE-100 4503792 NT Homo sapiens follicle sfirnulating hormone receptor (FSHR) mRNA 5024 10125 15197 3,1 1.OOE-100 5032104 NT Homo saple s small optic lobes (Drosophila) homolog (SOLH) mRNA 5024 10125 15198 3.1 1.6OE-100 5032104 NT Horno sapiens'small optic lobes (Drosophila) homolog (SOL11) mRNA 5277 10403 1.2 1.OOE-101 7110714 NT Homo saplens SEC14 (S. cerevislaeLAike 2 (SEC141-2), mIRNA 5277 10404 1.2 1.OOE-101 7110714 NT Homo sapiens SEC14 (S. cereAsiaelike 2 (SEC141-2), mRNA 671 '5827 10954 1.2 1.OOE-101 AB007915.2 NT Homo saplens mRNA for K1AAW6 protein, partial cds 688 5843 10979 1.5 1.OOE-101 7110734 NT Homo saplens ventral anterior homeobox 2 (VAX2), mRNA 688 5843 10980 1.5 1.0OE-101 7110734 NT Horno sapiens ventral anterior homeobox 2 (VAX2), mRNA 754 5906 11053 1.3 1.0015-101 7657454 NT Homo sapiens pescadillo (zebrafish) homolog 1, containing BRCT domain (PES1), mRNA 835 5984 11144 1,3 1.OOE-101 4503914 NT Homo sapiens phosphodbosylgiycinamide formyttransferase, phosphoribosylgiycinamide synthetase, phosphoribosylaminoimldazole synthetase (GART) mRNA 908 6054 11211 1,2 1,0012-101 Z20656.11 NT Homo saplens of cardiac alpha- myosin heavy chain gene 1036 6176 11328 0.6 1.OOE-101 A1221878.1 EST HUMAN g999e09.xl Soares NFL T GBC S1 Homo sapiens cDNA done IMAGE:1843336 3' 1712 6835 12015 0.9 1.0012-101 76621'83 NT Homo sapiens KIAA0569 gene product (K1AA0569), mRNA 1712 6835 12016 0.9 11.0OE-101 7662183 NT Homo saplens KIAA0569 gene product (K1AA0569), mRNA 1901 7019 12209 0.6 1.0OE-1011 4502996 NT Homo sapiens carboxypepddase Al (pancreatic) (CPA1) mRNA 2010 7124 12324 0.6 1.OOE-101 X97869.1 NT H.sapiens gene encoding La autoandgen 2301 7940 12612 0.6 1.OOE-101 5729892 NT Homo sapiens A kinase (PRKA) anchor protein 6 (AKAP6), mRNA 2572 7665 12867 1.6 1.0OE-101 Y07848.1 NT Horno sapiens EWS, gar22, rrp22 and bam22 genes 2714 7798 13000 0.9 1.OOE-101 AJ237744.1 NT Homo sapiens RIBOR gene (partial), exon.12 2714 7798 13001 0.9 1.0012-101 AJ237744.1 NT Homo sapiens RIBI1R gene (partial), exon 12 2925 8076 12.1 1.0OE-1011 AJ252312.1 NT Homo sapiens genomic downstream Rhesus box 3167 8316 13429 2.5 1.OOE-101 4885270 NT Homo saplens gammaglutamyltransferase 1 (GGT1) mRNA 3334 8478 13591 1.7 1.00EA 01 AW965556.11 EST HUMAN EST377629 MAGE resequences, MAGI Homo saplens cDNA 3354 7798 13000 1.8 1.OOE-101 AJ237744,1 NT Homo saplens RIBI1R gene (partial), exon 12 3354 7798 13001 1.8 1.OOE-101 A.1237744.1 NT Homo saplens RIBIR gene (partial), exon 12 3757 8895 13986 1.4 1.0OE-101 AF145712.1 NT Homo sapiens soluble neuropilin-l mRNA, complete cds 3800 8937 14031 4.8 1.0OE-101 AB022785.1 NT Homo sapiens ASH2L gene, complete cds, similar to Drosophila ash2 gene 4965 10068 15141 1.7 1.0OE-101 5921460 NT Homo sapi ns butyrophilin, subfamily 2, member AI (BTN2A1), mRNA 4965 10068 15142 1.7 1.OOE-101 5921460 NT Horno sapie s butyrophilin, subfamily 2, member Al (BTN2A1), mRNA 33 5519 10643 1.1 1.0OE-102 AL163303.2 NT Homc saplens chromosoffie 21 segment HS21 Cl 03 Page 149 of 196 Table 4

9 C 604 5762 10882 0.9 1.OOE-102 BE262470.1 EST_HUMAN 601108292F1 NIH MGQ_16 Homo saplens cIDNA done IMAGE:3344326 5' 748 5900 11047 0.7 1.OOE-102 D30612.1 NT Horno sapiens mRNA for repressor protein, partial cds 758 5910 11058 1.5 1.0OE-102 4557534 NT Homo sapiens down-regulated In adenoma (DRA) mRNA 1101 6239 11391 0.7 1.OOE-102 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 1398 6525 11694 33.0 1,00EA 02 BE408447.1 EST-HUMAN 601299982F1 N1H MGC_21 Homo sapiens cDNA clone IMAGE:3629901 5' 2157 7266 12476 0.7 1.OOE-102 AF182645.1 NT Homo sapiens chondrosarcoma- associated protein 2 (CSA2) mRNA, complete cds 2261 7367 12576 1.3 1.OOE-102 A1950528.1 EST HUMAN wx51e07.xl NCI-CGAP- Lu28 Horno sapiens cDNA done IMAGE:2547204 3'sImilar to SW:GG95 HUMAN 008379 GOLGIN-95.;contains element MER22 repetitive element; 2261 7367 12577 1.3 1.0012-102 A1950528.1 EST HUMAN wx51e07.xl NCI CGAP - Lu28 Homo sapiens cDNA done IMAGE:2547204 Ysimilar to SW:GG95 HUjAN Q08379 GOLGIN-95.;contains element MER22 repetitive element:

3026 8178 13279 1.5 1.00EA 02 7661979 NT Homo sapiens KIAA01 87 gene product (K1AA01 87), mRNA 4139 9265 14346 1.5 1.0OE-102 AL163207.2 NT Homo sapiens chromosome 21 segment HS21C007 4314 9437 14526 2.1 1.OOE-102 BE251310.1 EST-HUMAN 601107843F1 NIH MGQ_16 Homo sapiens cIDNA done IMAGE:3343882 V 5063 10161 15237 1.1 1.OOE-102 R66.488.1 EST-HUMAN Y132c04xl Soares placenta N1J2HP Homo sapiens cDNA clone IMAGE:140934 S' 93 5300 10426 2.8 1.00E.1 03 D87078.2 NT Homo saplens mRNA for KIAA0235 protein, pardal cds 201 5395 10521 0.8 1.0OE-103 5453793 NT Homo sapiens nudeolar p;otein (KKE/0 repeat) (NOP56) mIRNA 1578 6704 11874 0.8 1.OOE-1 03 AF012872.1 NT Homo sapiens phosphatidylinositol 4-kinase 230 (p14K230) mRNA, complete cds 1872 6990 12175 1,0 1.0OE-103 7657592 NT Homo sapiens smg GIDS-ASSOCIATED PROTEIN (SMAP), mRNA 1932 7049 12240 1.3 1.00EA 03 4502428 NT Homo sapiens bone morphogenefic protein 8.(osteogenic protein 2) (13MP8) mRNA 1932 7049 12241 1.3 1.0OE-103 4502428 NT Homo sapiens bone morphogenefic protein 8,(osteogenic protein 2) (13MP8) mRNA 2257 7363 12572 0.6 1.OOE-103 5803184 NT Homo sapiens synaptophysin-like protein (SYPL), mRNA 2401 7500 12707 0.8 1.OOE-103 AF060568.1 NT Homo sapiens promyelocytic leukernia zinc finger protein (PLZF) gene, complete cds 2578 7671 12872 0.9 1.OOE-103 N32770.1 EST HUMAN yw9ldO8.si Soares_piacenta-8togweeks-2NbHP8togW Homo sapiens cDNA clone IMAGE:259599 3' 3333 8477 13590 3.4 1.0OE-103 AW298245.1 EST-HUMAN Ul-H-BWO-ajt-h-11-0-Ul. sl NCI-CqAP-Sub. 6 Horno sapiens cDNA clone IMAGE:2733165 3, 3393 8535 13640 1.1 1.0012-103 A13040892.1 NT Homo sapiens mRNA for KIAA1459 protein, partial ods 3592 8731 1.3 1.0OE-103 AL048453.2 EST-HUMAN MFZp586,11 124jI 586 (synonym: hutel) Horno sapiens cIDNA clone DI(Rp586J1 124 3695 8834 2.4 1.OOE-103 AF023861.1 NT Macaca mulatta cyclophilin A mRNA, complete cds 3725 8862 13955 1.2 1.OOE-103 AA485663.1 EST-HUMAN ablOdl2.sl Stratagene lung (#937210) Homo sapiens cDNA done IMAGE:840407 Ysimilar to conta-ins element LTR10 repetitive element; 3922 9056 14143 3.0 1.OOE-103 T23683.1 EST HUMAN seq340 b4HB3MA-CotIO9+10- Bio Horno sapiens cDNA lone b4HB3MA-CotlOg+10-Bio-7 3 233 5424 10548 0.9 1.0OE-104 AL037549.3 EST-HUMAN DKIZZp5641-11072-rl 564 (synonym. Mbr2) Homo sapiens cDNA done DIKFZp5641-111072 5' 233 5424 10549 0.9 1.OOE-104 A1.037549.3 EST HUMAN DKFZP564H107:2-rl 564 (synonym: Mbr2) Homo sapiens cIDNA done DKRp5641-11072 5, 579 5739 10855, 1.1 1.0OE-104 AA496139.1 ES7_HUMAN zv51M 1.sl SoareE_testis-NHT Homo sapiens cIDNA done IMAGE:757197 Ysimilar to TR:G487738 G487738 PUTATIVE POTASSIUM CHANNEL SUBUNIT.; 5739 10856, 1.1 1.0OE-104 AA496139.1 EST HUMAN zv51fl.i.sl SoareeLtesds NHT Homo sapiens cIDNA done IMAGE:757197 Ysimilar to TR:G487738 G487738 PUTATIVE POTASSIUM CHANNEL SUBUNIT.; Page 150 of 196 Table 4

1845 6963 12149 0.7 1.0OE-104 4502428 NT Horno sapiens bone morphogenefic protein 8 (osteogenic protein 2) (13MP8) mRNA 1898 7016 1.9 1.0012-104 AW054828.1 EST HUMAN ws60d04.xl NCI - CGAP - Bm25 Horno sapiens cIDNA done IMAGE:2501575 3' similar to TR:Q13541 Q13541 412-BINDING PROTEIN l.; 2147 7257 12464 2.6 1.0OE-104 M34671.1 NT Human lymphocydc antigen CD59/MEM43 mRNA, complete cds 2318 7421 12629 1.2 1.OOE-104 AF167706.1 NT Homo sapiens cystelne-rich repeat-containing protein S52 precursor, mRNA, complete cds 2318 7421 12630 1.2 1.OOE-104 AF167706.1 NT Homo sapiens cystelne-rich repeat-containing protein S52 precursor, mRNA, complete cds 2387 7488 12691 0,6 1.OOE-104 5031570 NT Homo sapiens ARP2 (actin-related protein 2. yeast) homolog (ACTR2), mRNA 2842 7993 13091 7.6 1.OOE-104 M34671.1 NT Human lymphocytic antigen CD59/MEM43 mRNA, complete cds 2886 8037 2,8 1.OOE-104 Y1 1151.1 NT H.sapiens gene encoding phenylpyruvate tautomerase 11 3345 8489 1,5 1.0OE-104 AA319436.1 EST HUMAN EST21658 Adrenal gland tumor Homo sapiens cDNA S' end 3550 -8690 13792 1.0 1.OOE-104 AB033102.1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 3550 8690 13793 1.0 1.0OE-1 04 A13033102.1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 3861 8997 14096 0.9 1.OOE-104 AB032998.1 NT Homo sapiens mRNA for K1AA1 172 protein, partial cds 4280 9405 14489 4.3 1.OOE-104 X02761.1 NT Human mRNA for fibronectin (FN precursor) 4504 9623 14714 0.9 1.00EA 04 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 4504 9623 14715 0.9 1.0OE-1 04 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 4735 9846 2.8 1.OOE-104 AW054828.1 EST HUMAN ws60c104.xl NCI - CGAF_Bm25 Homo saplens cDNA done IMAGE:2501575 Ysimilar to TR:013541 013541 4E-BINDING PROTEIN l_.

277 7859 10594 1.0 1.00FE-105 4502166 NT Homo sapiens arnyloid beta (A4) precursor protein (protease nexin-II, Alzheirner disease) (APP), mRNA 419 5206 10309 5.7 1.OOE-105 4505150 NT Homo saplens MelsI (mouse) homolog (MEIS1) mRNA 1777 6898 12085 1.2 1.0OE-105 AL163280.2 NT Homo saplens chromosome 21 segment HS21 C080 1884 7002 12187 0.6 1.00IE-105 D50918.1 NT Human mRNA for KIAA0128 gene, partial cds 2142 7252 12460 0.6 1.OOE-105 J04977.1 NT Human Ku autolmmune anfigen gene, complete cds 2689 7776 0.9 1.OOE-105 AA584808.1 EST-HUMAN nol 0d05,sl NCI CGAP_Phel Homo sapiens cDNA done IMAGE:1 100265 X 2975 8126 2.6 1.OOE-105 AJ229041.1 NT Homo sapiens 959 kb contiq between AML1 and CBR1 on chromosome 21q22; segment 113 3307 8452 13564 0.9 1.OOE-105 7304922 NT Horno sapiens bromodomain agacent to zinc finger domain, 213 (BAZ2B), mRNA 3307 8452 13565 0.9 1.OOE-105 7304922 NT Horno saple s bromodomain adjacent to zinc finger domain, 2B (BAZ213), mRNA 4008 9139 14221 2.2 1.OOE-105 AW961688.1 EST HUMAN EST373761 MAGE rosequences, MAGG Homo sapient cIDNA 4855 9962 3.7 1.OOE-105 AIL163208.2 NT Homo sapiens chromosome 21 segment HS21C008 5044 10144 15222 1.0 1.0OE-105 AB018339.1 NT Homo sapiens mRNA for KIAA0796 protein, partial cds 5095 10193 15272 2.2 1.00EA 05 AB020673.1 NT Homo saplens mRNA for KIAA0866 protein, complete ods 5161 10144 15222 1.3 1.OOE-105 A13018339.1 NT Homo saplens mRNA for KIAA0796 protein, partial cds 5341 1.5 1.OOE-106 AW503208.1 EST HUMAN Ul-HF-BNO-akt-WO7-0-Ul.rl NIH MGC W Horno saplens cIDNA done IMAGE:3078348 5' 198 5392 10519 0.5 1.OOE-106 A1565065.1 EST HUMAN tg79c011.xl NCI C2Utl Homo saplens cDNA done IMAGE:2215008 3' 212 5405 10629 0.9 1.OOE-106 8922990 NT Homo sapiens hypothetical protein FI-J11 1316 (FI-J1 1316), mRNA 531 5695 10818 0.6 1.OOE-106 AW965556.1 EST.-HUMAN EST377629 MAGE resequences, MAGI Homo sapiens cDNA 5899 5749 10866 0.8 1.0OE-106 J00146.1 NT 1Human dihydrofolate reductase pseudogene (psi-hdl) Page 151 of 196 Table 4

C 590 5749 10866 1,2 1.OOE-106 J100146.1 NT Human dihydrofolate reductase pseudogene (p 1-hdl) 1508 6635 11804 1.0 1.OOE-106 AF1145712.1 NT Homo sapiens soluble neuropilin-1 mRNA, complete cds 1674 6798 11976 1.6 1.OOE-106 U48724.1 NT Human epidermal growth factor receptor (EGFIR) precursor-mRNA, exon 4, partial cds 1762 6883 12065 1.9 1.OOE-106 AA527446.1 EST-HUMAN ng41c05.sl NCI'CGAP- Co3 Homo sapiens cIDNA done IMAGE:937352 Ysimilar to contains element LTR3 repetitive element, 1762 6883 12066 1.9 1.OOE-106 AA527446.1 EST HUMAN n941c05.sl NCI__CGAF_Co3 Homo sapiens.dDNA done IMAGE:937352 Ysimilar to contains element LTR3 repetitive element; 2075 7187 12390 1.1 1.OOE-106 BE144286.1 EST HUMAN MRO-HT0165-140200-008- d10 HT0165 Homo sapiens cDNA 2269 7375 12584 3.0 1.OOE-106 4504184 NT Homo sapiens glutathione S- transferase theta 1 (GSTT11), mRNA 2456 7555 12761 0,6 1.0OE-106 AF003528.1 NT Homo saplens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 2557 7651 12854 1.3 1.OOE-106 U64675.2 NT Horno saplens sperm membrane protein BS-63 mRNA, complete cds 2559 7653 12856 0.7 11.0OE-106 BE313721.1 EST HUMAN 601149352F1 N1H MGC 19 Homo sapiens cDNA done IMAGE:3502117 5' 2729 7813 13019 1.5 1.OOE-106 A1276526.1 EST-HUMAN q176hl 0.xl Soares NhHIVIPu S1 Homo sapiens cDNA done IMAGE:1878307 3' 2795 6541 11715 1.1 1.0OE-106 4504184 NT Homo sap] ns glutathione S- transferase theta 1 (GSTTI), mRNA 2795 6541 11716 1.1 1.OOE-106 4504184 NT Homo saplens glutathione S- transferase theta 1 (GSTT11), mRNA 2911 856-1 13167 5.0 1.OOE-106 A13037747.1 NT Homo saplens mRNA for K1AA1 326 protein, partial cds 2911 8061 13168 5.0 1.OOE-106 AB037747.11 NT Homo sapiens mRNA for K1AA1 326 protein, partial cds 3143 8292 13399 2.4 11.00E. -106 8922965 NT Homo sapiens hypothetical protein FUll 1273 (FLJI 1273), mRNA 3143 8292 13400 2.4 1.OOE-106 8922965 NT Homo sapiens hypothetical protein FL.1 11273 (RJ1 1273), mRNA 3328 8472 13585 0.8 1.OOE-106 ABOO8681.1 NT Homo sapiens gene for activin receptor type llB, complete cds 3350 8494 13605 3.0 1.0OE-106 AWS03073.1 EST-HUMAN U1-1-IF-13P0p-aje-h-06- 0-Ul.rl NIH_MGC-51 Homo sapiens eDNA clone IMAGE:3074170 5 3394 8536 13641 1.0 1.OOE-106 AB033104.1 NT Homo sapiens mRNA for KIAA1278 protein. partial cds 3394 8536 13642 1.0 11.0OE-106 AB033104.1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 3840 8976 14076 1.8 1.OOE-106 BE145043,1 EST-HUMAN PM4-HT01 91 -281099- 002-d06 HT01 91 Horno sapiens cDNA 3952 9085 14166 7.2 1.OOE-106 AW974650.1 EST HUMAN EST386875 MAGE resequences, MAGN Homo sapiens cDNA 3952 9085 14167 7,2 1.OOE-106 AW974650.1 EST HUMAN EST386875 MAGE resequences, MAGN Homo sapiens cDNA 4497 9617 14708 1.2 1.00EA06 BE144286.1 EST-HUMAN MRO-HT0165-140200-008- d10 HT0165 Homo sapiens cDNA 5183 10275 1.2 1.OOE-106 141644.1 NT Homo saffiens dystrophin gene, exon 41 222 5414 10535 0,5 1.0OE-107 4506454 NT Homo sapiens reficulocalbin 1, EF- hand calcium binding domain (RCNII), mRNA 234 5425 1 22 1.OOE-107 AJ271735.1 NT Homo saplens Xq pseudoautosomal region; segment 112 264 5451 1.1 1.OOE-107 X60459.1 NT Human 1 FNAR gene for Interferon. alphalbeta receptor 606 W64 1.1 1.OOE-107 4826863 NT Homo sapiens neuronal cell adhesion molecule (NIRCAM) mRNA 614 5772 10893 0.6 1.OOE-107 AF155103.1 NT Homo sapiens W.REN-25 anfigen mRNA, partial cds 6' 9 5947 11097 0.9 1.0OE-107 X60459.1 NT Human IFNAR gene for Interferon alphalbeta receptor 6 6616 11176 1.2 11.0OE-107 X60459.11 NT Human IFNAR gene for Interferon alphalbeta receptor 68 818 953 6099 11256 3.6 1.0012-107 AF154121.1 NT Homo sapiens sodium-dependent high-affinity dicarboxylate transporter (NADC3) mRNA, 9 complete ods P796 6386 11547 0.8 1.OOE-107 AB032253.1 NT Homo sapiens BAZI B mRNA for bromodomain adjacent to zinc finger domain 1 B, complete 1 257 cds Page 152 of 196 1718 6841 12020 0.5 1.OOE-107 AF136275.1 NT Homo sapiens cathepsin Z precursor (CTSZ) gene, exon 3 1797 6917 12103 1.0 1.OOE-107 ABOO7922.2 NT Homo saelens mRNA for KIAA0453 protein, pardal cds 1797 6917 12104 1.0 1.OOE-107 A13007922.2. NT Homo sapiens mRNA for f<1AA0453 protein, partial cds 2161 7270 12481 1.3 1.OOE-107 U13729.1 NT Human dipepfidA peptdase IV (C1326) gene, ekon 20 2487 7584 12789 0,9 1.OOE-107 4759153 NT Homo sapiens synaptosomal- associated protein, 29k1D (SNAP29) mRNA 2487 7584 12790 0.9 1.OOE-107 4759153 NT Homo sapiens synaptosomal- associated protein, 2gkD (SNAP29) mIRNA 2976 8127 13233 1.9 1.OOE-107 AW842451.1 EST-HUMAN PM1-CN0031-190100-001- d03 CN0031 Horno sapiens cIDNA 2976 8127 13234 1.9 1.OOE-107 AW842451.1 EST-HUMAN PM1-CN0031-190100-001- d03 CN0031 Homo sapiens cDNA 3061 8212 13311 2.6 1.OOE-107 5902097 NT Homo sapiens SMT3 (suppressor of mif two 3, yeast) homolog 2 (SMT3H2), mRNA 3754 8892 13982 3.9 1.OOE-107 AF020671.1 NT Homo saplens myotubularin (MnMI) gene, exon 9 939 6086 11243 1.5 1.OOE-108 BE296042.1 EST-HUMAN 601177018F1 N11-1MC,_17 Homo sapiens cIDNA done IMAGE:3532348 5' 1244 6373 11531 0.5 1.0OE-1108 V18000.1 NT Homo sapiens NF2 gene 2012 -7126 0.7 1.00EA08 A1089548,1 EST HUMAN qb06f02.xl Soares_pregnant- uteru!_NbHPU Homo sapiens cDNA done IMAGE:1695483 3' 2282 7388 12595 2.7 1.OOE-108 AF138303.1 NT Homo sapiens decorin D mRNA, complete cds, alternadvely spliced 2282 7388 12596 2.7 1.0OE-108 AF138303.1 NT Homo sapiens decorin D mRNA, complete cds, altemabvely spliced 2378 7480 12682 2.6 1.OOE-108 BE206694.1 EST-HUMAN bb25b10.xl NIH-MGC-14 Homo sapiens cDNA done IMAGE:2963899 3' similar to qb:X53777 60S RIBOSOMAL PROTEIN L23 (HUMAN), qb:J05277 Mouse hexokinase mRNA, complete cds (MOUSE); 3158 8307 13417 1.9 1.0OE-108 4506354 NT Homo sapiens pregnancy-zone protein (PZP) mRNA 3158 8307 13418 1.9 1.OOE-108 4506354 NT Homo sapiens pregnancy-zone protein (PZP) mRNA 3742 8879 13969 0.9 1.OOE-108 5453855 NT Homo sapiens pedcenbiolar material 1 (PCM1) mRNA 4065 9192 14276 1.3 1.OOE-108 AW664438.1 EST-HUMAN W12a11.xl NCI - CGAP, GUI Homo sapiens cDNA done IMAGE:2972060 Ysimilar to SW:313PI-MOUSE P55194 SH3-BINDING PROTEIN 3BP-1, 4425 9546 14638 2.0 1.OOE-108 U72961.1 NT Human hepatocyte nuclear factor 4-alpha gene. axon 2 4425 9546 14639 2.0 1.00E508 U72961.1 NT Human hepatocyte nuclear factor 4-alpha gene, exon 2 4555 -9673 14764 2.1 1.OOE-108 AA186965.1 EST HUMAN zp66f01.rl Stratagene, endotheliat cell 937223 Horno sapiens cDNA clone IMAGE:625177 5 4699 9 F1 14908 2.9 1.OOE-108 7661979 NT Homo sapiens KIAA0187 gene product (K1AA0187), mRNA 4868 9975 15060 2.2 1.0OE-108 A.1008005.1 NT Homo sapiens PSW gene. altemadve transcript 58 5266 10388 0.9 1.0OW-109 D86974.1 NT Human mRNA for KIAA0220 gene, pardal cds 459 5625 10751 1.2 1.OOE-109 4507712 NT Homo saplens tetrahicopeptide repeat domain 2 (TTC2) mRNA 583 5743 10860 5.0 1.0OE-109 AB023216.1 NT Homo sapiens mRNA for KIAA0999 protein, pardal cds 583 5743 10861 5.0 1.OOE-109 AB023216.1 NT Homo sapiens mRNA for KIAA0999 protein, parlfal cds 1184 6317 11473 3.3 1.OOE-109 M28699.1 NT Homo saffiens nudeolar phosphoproteln B23 (NPM1) mRNA, complete cds 1185 6317 11473 1.7 1.0OE-109 M28699.1 NT Homo sapiens nudeolar phosphoprotein 823 (NPMI) mRNA, complete ods 1831 6950 12134 0.5 1.0OE-1 09 D13643.2 NT Homo saeiens mRNA for KIAA0018 protein, parlial cds 2194 7303 12517 0.7 1.OOE-109 AL163284.2 NT Horno saplens chromosome 21 segment HS21C084 2204 7313 12525 0.7 1.OOE-109 Y17123.1 NT Homo sapiens SISIF5/INIII gene, exon 6 2581 7674 12876 1.4 1.0OE-109 A1022328.1 EST_HUMAN ow95a01.xl Soares_fetal-tiveLspteen 1 NFLS_S 1 Homo saplens cDNA done IMAGE:1654536 Ysimilar to TR:00211i7 002197 CIRCULATING CATHODIC ANTIGEN.; Page 153 of 196 Table 4

2581 7674 12877 1.4 1.OOE-109 A1022328.1 EST HUMAN ow95a01.xl Soares fetal - liver-spleen-11 NIFILS-SI1 Homo sapiens cDNA done IMAGE:1654536 3 similar to TR:002197 002197 CIRCULATING CATHODIC ANTIGEN.; 2582 7675 12878 1,0 1.0OE-1109 4504206 NT Homo sapiens guanylate cyclase activator 1A (retina) (GLICAIA) mRNA 2907 8057 13162 1.3 1.OOE-109 BE243668.1 EST-HUMAN TCBAPID1367 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA Homo sapiens cDNA done TCBAP1367 2907 8057 13163 1.3 11.0OE-109 BE243668.11 EST HUMAN TWAP1 D1 367Pediatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project=TCBA Homo sapiens cDNA done TCBAP1367 3020 8172 13272 1.9 11.0OE-109 N85190.11 EST HUMAN J2816F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA done J2816 Ysimilar to ZINC FINGER PROTEIN ZNF43 3342 8486 13600 1,5 1.0012-109 AW893192.1 EST-HUMAN CM3-NNOOOO,-190400- 150-flO NNO009 Homo sapiens cDNA 3342 8486 13601 1.5 1.OOE-109 AW893192.1 EST - HUMAN CM3-NNO009-190400- 150-flO NNO009 Homo sapiens cDNA 3474 8615 13727 1.2 11.0OE-109 AF240698.1 NT Homo sapiens refinol dehydrogenase homolog lsoform-1 (RDH) mRNA, complete eds 3167 6905 1.5 1.0012-109 BE146144.1 EST HUMAN MRO-HT0209-110400-108-a04 HT0209 Homo sapiens cIDNA 3911 9046 14133 1.5 1.0OE-11 09 AB01 1181.2 NT Homo sapiens mRNA for KIAA0609 protein, partial cds, 3911 9046 14134 1.5 1.OOE-109 AB01 1181.2 NT Homo sapiens mRNA for KIAA0609 protein, partial cds 4054 9181 14264 3.7 1.OOE-109 A1655417.1 EST-HUMAN ts98e06.xl NCI WAP- GC6 Homo sapiens c[DNA done IMAGE:2239330 Ysimilar to WP:F53A2.8 CE116100; 4070 9197 14281 1.0 1.0012-109 AA662274.11 EST-HUMAN nu93c112.sli NCI WAP- Pr22 Homo sapiens cIDNA done IMAGE:1218262 Ysimilar to SW:WT2 HUMAN P30712 GLUTHATHIONE S-TRANSFERASE THETA 2; 4070 9197 14282 1.0 11.0OE-109 AA662274.1 EST HUMAN nu93c112.sl NCLWAP- Pr22 Homo sapiens cDNA done IMAGE:1218262 Ysimilar to SW:GTT2-H UMAN P30712 GLUTHATHIONE S-TRANSFERASE THETA 2; 4304 9428 14518 2.3 1.0OE-1109 4504206 NT Homo sapiens quanylate cyclase activator 1A (raVna) (GLIMA) mRNA 4496 9616 14707 1.2 11.0OE-109 7662083 NT Homo saplens KIAA0377 gene product (K1AA0377), mRNA 4839 9946 15035 1.0 1.OOE-109 R1 5400.1 EST HUMAN ya48e06xl Soares Infant bralb 1 NIB Homo saplens cDNA done IMAGE:53057 5 4984 10087 15158 0.9 11.0OE-11 09 BE293673.1 EST HUMAN 601186922F2 NIH MGC 15 Horno sapiens cDNA done IMAGE:2959636 5' 4984 10087 15159 0.9 1.0OE-109 BE293673.1 EST HUMAN 601186922F2 NIH_MGC_15 Homo sapiens cIDNA done IMAGE:2959636 5' 3 5212 10313 0.5 1.OOE-110 7549804 NT Homo sapiens delodinase, iodothyronine, type 11 (D102), transcript variant 2, mRNA 34 5243 10349 1.3 1.OOE-110 5803073 NT Homo sapiens leudne-zipper-like transcriptional regulator, 1 (LZTR1), mRNA 34 5243 10350 1.3 1.OOE-110 5803073 NT Homo sapiens leucine-Apper-like transcriptional regulator, 1 (LZTR1), mRNA 5281 10408 10.0 1.0OE-11 10 C04498.11. EST-HUMAN C04498 Human heart cDNA (YNakamura) Homo sapiens cDNA clone 3NHC3467 104 5212 10313 0.6 1.OOE-110 7549804 NT Homo sapiens delodinase, lodothyronine, type 11 (D102), transcript variant 2, mRNA 291 5477 10608 0.9 1.0OE-11 10 D87291.1 NT Human mRNA for Inward rectifier potassium channel, complete cds 516 5680 10801 1.2 1.OOE-1 10 U84550.1 NT Human dystrobrevin (DTN) gene, exon 20 1163 6298 11453 1.1 1.00EA 10 5031620 NT Homo sapiens caldtonin receptor- like (CALCRL) mRNA 1258 6387 11W 1.0 1.OOE-110 AB032253.1 NT Homo sapiens BAZ1 S mRNA for bromodomain adjacent to zinc finger domain 1 B, complete ods 1879 6997 12182 0.5 11.0OE-110 BE379477.1 EST-HUMAN 601237545F1 NIH MGCL44 Homo saplens cIDNA done IMAGE:3609683 5' 2810 7961 1.0 1.0OE-1110 4503098 NT Horno sapiens chondroitin sulfate proteoglycan 4 (melanoma-assodated) (CS1PG4), mRNA 3048 8199 1.1 1.0OE-110 U78027.1 NT Homo saplens Bruton's tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like ribosomal protein (L441---)and FTP3 (FTP3) genes, complete ods 1 Page 154 of 196 Table 4

3966 9098 14182 0.9 1.0OE-110 BE018556.1 EST HUMAN bb82a05.yl NIH - MGC 10 Homo saplens cIDNA clone iMAGE:3048548 5'similar to TR:060312 060312 KIAA0566 PROTEIN; 4533 9652 14739 2,1 1.OOE-110 A1017213.1 EST HUMAN ou32b10.xl Soares-NFL- T-WC---S1 Homo sapiens cIDNA clone IMAGE:1627963 3'sImilar to SW:W21_RAT P52591 NUCLEAR ENVELOPE PORE MEMBRANE PROTEIN POM 121 4898 10005 2.3 1.OOE-110 7662441 NT Horno saplens KIAA1002 protein (K1AA1002), mRNA 168 5362 5.7 1.00EA 11 U43701.1 NT Human dbosomal protein L23a mRNA, complete cds 189 5383 10510 1.0 1.00EA 11 4758807 NT Homo saplens ras GTPase activating protein.like (NGAP) mRNA 726 5880 11021 1.2 1.00EA 11 8393092 NT Homo sapiens cat eye syndrome critical region gene 1 (CECRI). mRNA 911 6057 11216 25.0 1.0OE-11 11 M25142.1 NT Human cardiac alpha-myosin heavy chain (MYH6) gene, exons 32 to 34 1564 6691 11860 2.0 1.0OE-11 11 AA213434.1 EST-HUMAN zq93f12.rl Stratagene hNT neuron (#937233) Homo sapiens cDNA done IMAGE:649583 5' 1564:6.. 691 11861 2.0 1.00EA 11 AA213434.1 EST-HUMAN zq93f12.rl Stratagen.e hNT neuron (#937233) Homo saplens cDNA done IMAGE:649583 5' 3642 8781 13874 1.2 1.0OE-11 11 6912641 NT Homo sapiens sex comb on midleg homolog 1 (SCMH1),_mRNA 3642 8781 13875 1.2 1.00EA 11 6912641 NT Homo sapiens sex comb on midleg homolog 1 (SCMH1), mRNA 4080 92071 14292 1.1 1.0OE-1 11 7661569 NT Horno sapiens DKFZP434D1 56 protein (1)KFZP434D1 56), mRN 4235 9359 14441 4.5 1.0OE-1 11 K02268.1 NT Human enkephalin B (enkB) gene, exon 4 and V1ank and homplete cds 592 5750 10867 0.9 1.00E.1 12 4501854 NT Homo sapiens acetyl-Coenzyme A carboxylase beta lACB), mRNA 594 5752 10869 1.5 1.00EA 12 U29103.1 NT Human steroidogenic acute regula!2D protein (StAR gene, exon 5 594 5752 10870 1.5 1.0OE-1 12 U29103.1 NT Human steroldogenic acute regulatory protein (StAR) gene, exon 5 985 6130 11285 1.0 1.0OE-112 AF157623.1 NT Homo sapiens HTRA sedne protease (PRSS1 1) gene, complete cds 1046 6186 11337 0.6 1.0OE-1 12 P52742 SWISSPROT ZINC FINGER PROTEIN 135 1658 6782 11956 2.1 1.OOE-1 12 7662125 NT Homo sapi ns KIAA0440protein (K1AA0440), mRNA 1658 6782 11957 2.1 1.00EA 12 7662125 NT Homo saplens KtAA0440 protein (K1AA0440), mRNA 2460 7559 12763 0.9 1.0OE-11 12 AF0Q4713.1 NT Homo sapiens DNA-dependent DNA polymerase (hREV3) mRNA, partial cds 3135 8284 13391 1.2 1.0OE-1 12 AF247662.1 NT Homo sapiens SR-related protein LD2 mRNA; partial ods 3809 'BM 14042 0.8 1.00EA 12 BE076073.1 EST-HUMAN MR2-BT0590-090300-113- f09 BT0590 Homo saplens cIDNA 4646 9759 14853 5.1 1.00EA 12 A13037832.1 NT Homo sapiens mRNA for KIAA1411 protein, partial cds 4646 9759 14854 5.1 1.00EA 12 A13037832.1 NT Homo saplens mRNA for KIAA1411 protein, partial ods 5170 10264 15336 0.9 1.00EA 12 9055269 NT Homo saplens low density lipoproteln receptor related protein-deleted In tumor (LRPDiT), mRNA 725 5879 11019 1.3 1.0OE-113 A1365586.1 EST HUMAN ao95f01.xl Schiller meningioma Homo sapiens cDNA done IMAGE:1953625 3, 725 5879 11020 1.3 1.OOE-113 A1365586.1 EST HUMAN ao95101.xl Schiller meningloma Homo saplens cDNA done IMAGE:1 953625 3, 927 6074 11232 2.2 1.00EA 13 M1 1965.1 NT Human X-linked phosphoglycerate kinase gene, exon 8 1523 6650 11818 1.1 1.0OE-11 13 A1365586.1 EST-HUMAN ao95101.xl Schiller meningloma Homo sapiens cDNA done IMAGE:1 953625 3, 2048 7161 12365 1.2 1.00EA 13 5453699 NT Homo sapiens Interferon regulatory factor 6 (iRF6) mRNA 2405 7504 12711 1.0 1.00EA 13 AJ006976.1 NT Horno sapiens PLP gene 3091 8242 13345 2.3 1.00EA 13 AJ223948.1 NT Homo saplens mRNA for putative RNA helicase. X end 5035 10135 15209 1.0 1.00EA 13 7657065 NT Homo saplens v-ets avian erythrobtastosts virus E26 oncogene related (ERG),. mRNA 503S00135 15210 1.0 1.00EA 13 7657065 NT Homo saplens v-ets avian erythroblastosis xlrus E26 oncogene related (ERG), m - - U Page 155 of 196 Table 4

9 cj 629 5788 10911 2.6 1.0012-114 T70551.1 EST HUMAN ydl5c01.sl Scares fetal liver spleen I NFILS HomosapienicDNA done IMAGE:108288 3' similar to gb:A21187 ALPHA-2-MACROGLOBULIN PRECUI RSOR (HUMAN);contains Alu repetitive element; 1055 6194 11347 1.3 1.00EA 14 8923087 NT Horno sapiens hypothetical protein FLJ20080 (FLJ20080), mRNA 1291 6420 11585 1.2 1.00EA 14 7657529 NT Homo sapiens rhabdold tumor deletion region protein 1 (RTDR1), mRNA 1648 6774 11945 2.0 1.00EA 14 6679073 NT Horno saplens nudeoporin-like protein 1 (NI-P 1), rnRNA 1980 7095 12297 1.2 1.00EA 14 BE165972.1 EST-HUMAN MR3-HT0487-150200-116- d01 HT0487 Homc, saplens cDNA 2773 5247 10355 0.8 1,00EA 14 A13033102.1 NT Homo sapiens mRNA for K1AA1 276 protein, pardal cds 2773 5247 10356 0.8 1.00EA 14 A13033102.1 NT Horno saplens mRNA for KIAA1276 protein, partial cds 3092 8243 13346 2.3 1.00EA 14 X04086.1 NT Human gene for catalase (EC 1. 11. 1.6) exon 2 mapping to chromosome 11, band pl 3 3934 9068 14153 2.0 1.00EA 14 AF149773.1 NT Homo sa ens NOD1 protein (NOD1) gene, exons 1, 2. and 3 5120 10216 15294 1,2 1.OOE-114 AA194468.1 EST HUMAN zq05e05xl Stratagene muscle 937209 Homo saplens cDNA clone IMAGE:628832 W similar to contains MER224 MER22 repetitive element; 5121 10217 15295 2.3 1.OOE-114 AF004849.1. NT Horno sapiens PKY protein kinase -mRNA, complete cds 21 5230 10333 1.2 1.00EA 15 4758111 NT Horno sapiens HLA-B associated transcript-1 (D6S81 E) mRNA 5321 10452 1.0 1.OOE-115 4505938 NT Homo sapiens polymerase (RNA) 11 (DNA directed) polypepfide A (220kD) (POLR2A) mRNA 129 5325 0.7 1.00EA 15 4557887 NT Homo sa lens keratin 18 (KRT1 8) mRNA 289 5475 10605 0.7 1.00EA 15 AW804759.1 EST-HUMAN QV4-UM0094-300300-156- b08 UM0094 Homc, sapiens cIDNA 374 5550 10683 1.0 1,00EA 15 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTS AND CINIC HOMOLOG 1) (HA2303) 525 5689 10810 1.1 1.OOE-115 A1339206.1 EST HUMAN qtO6f01.xl INIC1 CGAP - GC4 Homo sapiens cIDNA clone IMAGE:1946809 3' similar to TR:000536 000536 TTIF-1 INTERACTING PEPTIDE 5; 525 5689 10811 1.1 1.0OE-115 A1339206.1 EST HUMAN qt06f01.xl NCI CGAP GC4 Homo sapiens cIDNA done IMAGE:1946809 Ysimilar to TR:000536 0C0536 TTF-1 INTERACTING PEPTIDE 5; 769 5921 11069 0,6 1.0012-115 5174702 NT Homo sapiens transforming growth factor beta-activated kinase-binding protein 1 (TAB1), mRNA 769 5921 11070 0.6 1.00EA 15 5174702 NT Homo sapiens transforming growthfactor beta-activated kinase-binding protein 1 (TAB1), mRNA 771 592$ 11072 17.0 1.00EA 15 4503794 NT Homo sapiens ferritin, heavy poiyl?epdde 1 (FTH1) mRNA 1539 6665 11833 0.9 1.00EA 15 AF229180.1 NT Homo saplens alpha- aminoadipate semialdehyde synthase mRNA, complete cds 1539 6665 11834 0.9 1.OOE-115 AF229180.1 NT Homo sapiens alpha- aminoadipate semialdehyde synthase mRNA, complete cds 1785 6905 12093 1.1 1.OOE-115 AJ277892.1 NT Homo sapiens partial TTN gene for fifin 1798 6918 12105 0.5 1.00EA 15 U78027.1 NT Homo sapiens Bruton's tyrosine kinase (M). alpha-D-galactosidase A (GLA), L44-like ribosomal protein (1.441---)and- FTP3 (FTP3) genes, complete cds 2034 7147 12349 0.9 1.OOE-115 BE296353.1 EST-HUMAN 601174384F1 NIH MGC 17 Horno sapiens cIDNA done IMAGE:3529773 5, 2034 7147 12350 0.9 1.00EA 15 BE296353.1 EST-HUMAN 601174384F1 NIH MGC 17 Homo sapiens cIDNA done IMAGE:3529773 V 2704 7878 12990 0.8 1,00EA 15 4758111 NT Homo sapiens HLA-B as7o"clated transcript-1 (D6S81 E) mRNA 2820 7971 1.8 1.OOE-115 AW804759.1 EST HUMAN QV4-UM0094-300300-156-b08 UM0094 Homo sapiens cDNA 3077 872-8 13327 2.1 1.00E.1 15 AJ245922.1 NT Homo saplens mRNA for alphatubulin 8 BA8 gene) 3077 8228 13328 2.1 1.00EA 15 AJ245922.1 NT Homo sapiens mRNA for alpha- tubulin 8 (TUBA8 gene) Page 156 of 196 Table 4

I-- C.) 3427 8569 13681 4.0 1.0OE-115 AJ277892.1 NT iHomo sapiens partial TTN gene for titin 3956 9089 14172 4.0 1.00EA 15 A13002348.2 NT Homo sapiens mRNA for KIAA0350 protein, partial ods 4169 9295 14376 1.1 1.0OE-1 15 AL137163.1 NT Novel human gene mapRing to chomosome X 4301 9425 14515 3.4 1.00EA 15 6912659 NT Homo;aplens s!r2-like 3 (SIRT3), mRNA 4335 9458 14546 3.8 1.00EA 15 4758279 NT Horno sapiens EphA4 (EPHA4) mRNA 4578 9694 14786 2.6 1.0OE-1 15 AL096857.1 NT Novel human mRNA from chromosome 1, whichhas similarities to BAT2 genes 4578 9694 14787 2.6 1.0OE-1 15 A1.096857.1 NT Novel human mIRNA from chromosome 1, which'has similarities to BAT2 genes 4813 9922 15014 3.0 1.0OE-11 15 AL.163268.2 NT Horno sapiens chromosome 21 segment HS21 C068 4813 9922 15015 3.0 1.OOE-115 AL.163268.2 NT Homo sapiens chromosome 21 segment HS21 C068 560 5722 10838 1.4 1.OOE-116 BE275502.1 ESTHUMAN 601121347F1 N1H MGC 20 Homo sapiens cDNA done IMAGE:2988875 V 783 5935 11084 1.3 1.0OE-1 16 4507334 NT Homo sapiens synaptojanin 1 (SYNA), mIRNA 841 5990 6.9 1.0OE-1 16 4507334 NT Horno, sapiens synaptojanin 1 (SYNA), mRNA 1952 7069 12265 0.9 1.0OE-1 16 5174478 NT Horno, sapiens pericentrin (PCNT) mRNA 1952 7069 12266 0.9 1.0OE-11 16 5174478 NT Horno saplens pericentrin (PCNT) mIRNA 2050 7933 12366 1.0 1.0OE-1 16 M19824.1 NT Human apolipoeroteln 8-100 (apoB) gene, exons 17 and 18 2050 7933 12367 1.0 1.OOE-116 M19824.1 NT Human apollpoproteln 8-100 (apoB) gene, exons 17 and 18 2259 7365 12574 0,7 1.00EA 16 5453941 NT Homo sapiens protein phosehatase, EF hand calcium-binding domain 1 (PPEF1) mRNA 2293 7397 0.5 1.00EA 16 U78308.1 NT Human olfactory receptor olfr117-201- 1 (OR17-201-1) gene, olfactory receptor olfr17-32 (01117 32) gene and olfactory receptor pseudo-olfrl7-01 (OR17-01) pseudogene, complete ods 2407 7506 12713 1.6 1.OOE-1 16 A13018333.1 NT Homo sapiens mRNA for KIAA0790 protein, partial cds 3137 8286 13392 4.7 1.0OE-1 16 L77570.1 NT Homo sapiens DIGeorge syndrome critical region, centromeric end 3137 8286 13393 4.7 1.00EA 16 1.77570.1 NT Horno sapiens DiGeorge syndrome critical region, centromeric end 4281 9456 14490 2.0 1.00EA 16 5031954 NT Homo sapiens sodium phosphate transporter 3 (NPT3) mRNA 4747 9858 14950 2.2 1.OOE-116 A1907096.1 EST-HUMAN PM-BT135-070499-016 BT135 Homc sapiens cDNA 5122 10218 15296 1.2 1.00EA 16 AJ243213.1 NT Homo, sapiens partial 5-HT4 receptor gene, exons 2 to 5 267 5454 10579 1.4 1.0OE-11 17 4507500 NT Homo, saplens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 546 5709 10828 1.2 1.00EA 17 4826636 NT Horno, sapiens acetyt-Coenzyme A carboxylase alpha (ACACA), mIRNA 11 OU 6V 11 7906 11353 0.5 1.00EA 17 AF124393.1 NT Mus musculus fragile-X- related protein 1 (Fxrl h) gene, exons 13a through 15 1719 6842 12021 6.3 1.0OE-1 17 AF123320.1 NT Homo sapiens lymphocyte activation-associated protein mRNA, comElete cds 1772 6893 12081 2.1 1.00EA 17 H26662.1 EST-HUMAN y114d05.sl Soares breast 2NbHBst Homo sapiens cIDNA done IMAGE:1 58217 Ysimilar to gbW09850 ZINC FINGER PROTEIN 76 (HUMAN); 1790 6910 12096 0.8 1.00EA 17 M19816.1 NT Human apolipoprotein B-100 (apoB) gene, exon 10 2164 7273 12484 1.1 1.0OE-1 17 AW957699.1 EST-HUMAN EST369769 MAGE resequences, MAGE Horno saplens cIDNA 3230 8378 13485 1.8 1.0OE-1117 AA978114.1 EST-HUMAN op32cl 1M Soares NFt, GBC S1 Homo sapiens cIDNA done IMAGE:1 578548 3' 3908 9043 14131 3.6 1.00EA 17 AA316723.1 EST HUMAN EST188414 HCC cell line (metastasis to liver In mouse) 11 Homo sapiens cDNA Vend similar to dbosomal protein L29 4247 9372 14452 1.9 1.0012-117 8659564 NT Horno sapiens collagen, type IV, alpha 5 (Alport syndrome) (COL4A5), mRNA 4475 9595 14690 1.9 1.00EA 17 AL.042120.1 EST-HUMAN DKFZp434C1 120-rl 434 (synonym. htes3) Homo saplens cIDNA done DI(FZp434C1 120 5' 4622 9736 14831 1.4 1.00EA 17 X89670.1 NT H.sapiens mRNA for TIPM1 6 protein Page 157 of 196 Table 4

00 r 4622 9736 14832 1.4 1.00EA 17 X89670.1 NT H.sapiens mRNA for TPC1R1 6 protein 4705 9817 14914 9.2 1.OOE-117 AF134304.2 NT Homo sapiens Scar2 (SCAR2) gene, partial cds 4705 9817 14915 9.2 1.00EA 17 AF1 34304.2 NT Homo sapiens Scar2 (SCAR2) gene, partial cds 4856 9963 15048 3,4 1.OOE-117 AB020673.1 NT Homo sapiens mRNA for KIAA0866 protein, complete ods 64 5272 10395 3.3 1.OOE-118 AF161500.1 NT Homo sapiens HSPC151 mRNA, complete cds 5296 10422 0.9 1.00EA 18 AL045854.1 EST HUMAN DOZp434105Q._rl 434 (synonym: htes3) Homo sapiens cDNA done DOZp4341056 5' 355 5534 10664 0.5 1.00EA 18 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 506 5670 10793 1.7 1.00EA 18 7657016 NT Homo sapiens hypothetical protein (DJ328E19.C1.1), mRNA 896 7932 11199 1.0 1.00EA 18 5174680 NT Homo sapiens sine oculls homeobox (Drosophila) homolog 1 (SIX1) mRNA 2186 1295 12507 0.9 1.00EA 18 BE389705.1 EST HUMAN 601281947F1 NIH MGCj44 Horno saplens cDNA clone IMAGE:3604019 5' 2186 7295 12508 0.9 1.00EA 18 BE389705.1 ESTHUMAN 601281947F1 N1H MGC 44 Horno sapiens cDNA clone IMAGE:3604019 5' 2186 7295 12509 0.9 1.00EA 18 SE389705.1 EST HUMAN 601 281947F1 N1H MGC_44 Horno sapiens cDNA done IMAGE:3604019 5' 2281 7387 1.4 1.OOE-118 AW951729.1 EST-HUMAN EST365799 MAGE resequences, MAGB Homo sapiens cDNA 2711 7796 12997 0.9 1.0OE-1 18 U07000.1 NT Human breakpoint duster region (BCR) gene, complete cds 2711 7796 12998 0.9 1.00EA 1 a U07000.1 NT Human breakpoint cluster region (BCR) gene, complete cds 3066 8217 3.7 1.0012-118 Y13932.1 NT Homo saplens PRKY exon 7 3159 8308 13419 4.5 1.0OE-1 18 A1347694.1 EST HUMAN gpOlf05.xl NCI CGAP K1d5 Homo sapiens cDNA done IMAGE: 1916769 3' 3159 8308 13420 4,5 1.OOE-118 A1347694.1 EST-HUMAN qp01f05.xl NCI CGAP K1d5 Homo sapiens cDNA done IMAGE:1916769 3, 3998 9129 14211 7.7 1.00EA 18 D23660.1 NT Human mRNA for ribosomal protein, complete ods 741 5894 11040 0.8 1.OOE-119 AF170492.1 NT Homo sapiens chloride channel CLC4 (C1C4) mRNA, complete cds 1021 7905 11313 0.5 1.00Ei 19 7705607 NT Homo sapiens CGIA 05 protein (LOC51 011), ffiRNA 1891 7008 12195 0.8 1.OOE-119 AB023147.1 NT Homo sapiens mRNA for KIAA0930 protein, partial cds 3064 6215 13315 1.8 1.0OE-119 8922205 NT Homo sapiens hypothetical protein FLJ10052 (FU10052). mRNA 3202 8350 1,1 1.OOE-119 AA916760.1 EST-HUMAN on10b05.sl NCI-CGAP-Lu5 Homo sapiens cDNA done IMAGE:1556241 3'similar to WRE04F6.2 CE01214; 3870 9006 14105 1.2 1.OOE-119 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 5111 10207 15285 1.0 1.OOE-119 AA077394.1 EST-HUMAN 7B14F03 Chromosome 7 Fetal Brain cDNA Library Homo sapiens cDNA clone 7B14F03 237 5427 10554 1.4 1.OOE-120 A13018301.1 NT Homo sapiens mRNA for KIAA0758 protein partial ods 298 5484 10614 1.3 1.OOE-120 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 1025 6165 11319 0.6 1.OOE-120 AF248540.1 NT Homo sapiens Intersectin 2 (SH3D1 B) mRNA, complete cds 1025 6165 11320 0.6 1.0012-120 AF248540.1 NT Homo sapiens intersectin 2 (SH3D1 B) mRNA, complete cds 1406 653 11704 1.6 1.OOE-120 N44873.1 ES',HUMAN)y4Ogl 2.rl Soares melanocyte 2NbHM Horno sapiens cDNA done IMAGE:273766 S' 1583 6709 11880 1.4 1.OOE-120 AF167706.1 NT Homo sapiens cystelne-rich repeat-containing protein S52 precursor, mRNA, complete cds 1684 6808 11990 0.7 1.OOE-120 5729777 NT Homo saplens collagen, type X11, alpha 1 (COL12A1), mikNA 1684 6808 11991 0.7 1.OOE-120 5729777 NT Homo sapiens collagen, type X11, alpha 1 (COLI2A1), mRNA 2060 7172 12379 0.9 1.OOE-120 5174574 NT Homo saplens myeloldllymphoid or mixed-lineage leukernia (trithorax (Drosophila) homolog); translocated to, 4 (MI-LT4) mRNA 2060 7172 123801 0.9 1.0OE-120 5174574 NT Homo sapiens myeloidllymphold or mixed-lineage leukernia (trithorax (Drosophila) homolog); translocated to, 4 (MI.LT4) mRNA 7580 12782 0.8 1.0OE-120 4755124 NT Homo sapiens aquapodn 4 (ACIP4), splice variant b, mRNA Page 158 of 196 Table 4 (3- 3267 5484 10614 1.1 1.0OE-120 i 4507334 NT Homo sapiens synaptojanin 1 (SYNJ 1), mRNA 4260 9385 14468 1.2 1.OOE-120 AF056490.1 NT Homo sapiens cAMP-specJflc phosphodiesterase 8A (PDE8A) mRNA, partial cds 4260 9385 14469 1.2 1.OOE-120 AF056.490.1 NT Horno, sapiens cAMP-specific phosphodiesterase 8A (PDE8A) mRNA, partial cds 4543 9662 14752 1,2 1.OOE-120 AW378049.1 EST-HUMAN MRO-HT0209-301199-101- d04 HT0209 Hom6sdpiens cIDNA 4563 9681 14774 2.8 1.OOE-120 AF098463.1 NT Homo sapiens stanniocaldn (STC) gene, partial cels 4563 9681 14775 2.8 1.OOE-120 AF098463.1 NT Homo saplens stanniocalcin (STC) gene, partial cds 5168 10262 15333 0.9 1.OOE-120 A1190903.1 EST HUMAN qd61f03.xl Soares tesds NHT Homo sapiens cIDNA done IMAGE:1733981 3' 67 5274 10399 1.0 1.OOE-121 Y18000.1 NT Horno, saplens NF2 gene.

707 7896 10997 1.2 1.0OE-121 5032192 NT Homo, sapiens TNF receptorassociated factor 1 (TRAF1) mRNA 1556 6683 11851 1.0 1.OOE-121 A13011153.1 NT Homo sapiens mRNA for KIAA0581 protein, partial cds 1923 7040 12229 0.9 1.0OE-121 4755139 NT Homo sapiens inositol polyphosphate-4-phosphatase, type 1. 107ki) (INPP4A), splice variant a, mIRNA 1923 7040 12230 0.9 1.OOE-121 4755139 NT Homo sapiens Inositol polyphosphate-4-phosphatase, type i, IOAD (INPP4A), splice variant a. mRNA 2055 7167 12373 1.2 1.0OE-121 6006005 NT Homo sapiens glutamate receptor, metabotroplc 1 (GRM1) mRNA 3042 8193 13295 3.1 1.OOE-121 Y19208.1 NT Homo saplens hHb3 gene for hair keratin, exons 1 to 9 3042 8193 13296 3.1 1.OOE-121 Y19208.1 NT Homo sapiens hHb3 gene for hair keratin, exons 1 to 9 3492 8633 13745 1.1 1.OOE-121 AB037758.1 NT Homo sapiens mRNA for K1AA1 337 protein, partial cds 3492 8633 13746 1.1 1.OOE-121 A13037758.1 NT Homo sapiens mRNA for KIAA1337 protein, partial cds 1 3624 8763 13857 6.6 1.0OE-121 AF155156.2 NT Homo sapiens adaptor-related protein complex A12-4 epsilon subunit mRNA, complete cds 4236 9360 14442 1.2 1.0OE-121 A1263294.1 EST_HUMAN qx57b01.xl NCI CGAP Panll Homo sapiens cDNA done IMAGE:2005417 3' 4565 9683 14777 5.5 1.0OE-121 4506118 NT Homo sapiens prospero-related homeobox 1 (PROX1) mIRNA 4901 10008 15088 2.6 1.OOE-121 X919377 NT H.sapiens ECE-1 gene (exon 17) 5050 10150 15227 1.0 1.OOE-121 A1904151.1 EST-HUMAN CM-BT043-090299-075 BT043 Homo saplens cDNA 333 5514 10639 0.8 1.0OE-122 AF1 14488.1 NT Homo sapiens intersectin short Isoform (ITSN) mRNA, complete ods 864 6012 11173 1.0 1.0012-122 AF114488.1 NT Homo sapiens intersectin short Isoform (ITSN) mRNA, complete cd 1201 6333 11489 1.2 1.0OE-122 M20707.1 NT Human kappa-lmmunoglobulln germline pseudogene (Chr22.4) variable region (subgroup V kappa 11) 1667 6791 11967 1.3 1.OOE-122 AF167706.1 NT Homo sapiens cysteine-dch repeat-containing protein S52 precursor, mRNA, complete ods 2441 7540 12747 3.8 1.OOE-122 4506662 NT Homo sapiens dbosomal protein L8 (RPL8) mRNA 2441 7540 12748 3.8 1.OOE-122 4506662 NT Homo saplens dbosomal protein L8 (RPL8) mRNA 4738 9849 14941 1.8 1.OOE-122 4502166 NT Homo saplens amyrfold beta (A4) precursor protein (protease neyin-li, Akhelmer disease) (APP), mIRNA 4918 10025 1.2 1.OOE-122 AW504645.1 EST HUMAN Ul-HF-BNO-alf-a-03-0-Ul.rl N1H MGC 50 Homo sapiens cDNA done IMAGE:3079948 5' 181 5375 10500 1.1 1.OOE-123 U31519.1 NT Human phosphoenolpyruvate carboxyikinase (PCK1) gene, promoter region and partial eds 996 6138 11292 1.3 1.0OE-123 AL163249.2 NT Homo sapiens chromosome 21 segment HS21 C049 A 6145 11299 1.7 1.OOE-123 5803114 NT Homo saplens inner membrane protein, mitochondrial (mitofilin) (IMMT), mIRNA 0 Page 159 of 196 Table 4

1218 6348 11508 1.1 1.OOE-123 4505818 NT Homo sapiens phosphaddylinositol4-phosphate 5-kinase, type 11, beta (P1P5K213) mRNA, and translated products 1218 6348 11509 1.1 1.OOE-123 4505818 NT Homo sapiens phosphaddylinositol- 4-phosphate 5-kinase, type 11, beta (P1P5K2B) mRNA, and translated products 1293 6422 11588 0,9 1.0OE-1123 AW362922.1 EST HUMAN RC1-CT0295-241 199-01 11-a03 CTO295 Homo sapiens cDNA 1439 6566 11743 0.6 1.0OE-1123 AJ3886411.1 NT Homo saplens partial mRNA for immunoglobulin kappa chain variable region (IGVK gene), sample GN02 2052 7164 12369 1.1 11.0OE-123 4502072 NT Homo sapiens amelogenin (Y chromosome) (AMELY) mRNA 2052 7164 12370 1.1 1.0OE-1123 4502072 NT Homo sapiens amel RNA 2052 7164 12371 1.1 11.0OE-1123 4502072 NT Homo sapiens amei,,jenin (Y chromosome) (AMELY) mRNA 2267 7373 1.6 11.0OE-123 7705962 NT Homo sapiens RAB9-like protein (LOC51209), mRNA 268 5455 10580 1.1 1.0012-1124 4507500 NT Homo sapiens T-cell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 268 5455 10581 1.1 1.OOE-124 4507500 NT Homo sapiens T-call lymphoma Invasion and metastasis 1 (TIAM1) mRNA 273 5461 0.7 1.0OE-1124 D87675.1 NT Homo sapiens DNA for arnyloid precursor protein, complete cds 477 5643 10770 0,7 1.0OE-1124 AL1 63246.2 NT Homo sapiens chromosome 21 segment HS21 C046 676 5831 10961 0.7 1.OOE-124 AA3975511.1 EST-HUMAN zt81 b04.rl Stratagene schizo brain S1 1 Homo sapiens cDNA done IMAGE:728719 Csimilar to TR:G300482 G300482 P01---=REVERSETRANSCRIPTASE HOMOLOG (RETROVIRAL ELEMENT}; 676 5831 10962 0.7 1.OOE-124 AA3975511.1 EST-HUMAN zt81b04.rl Stratagene schizo brain S11 Homo sapiens cIDNA done IMAGE:728719 5' similar to TR:G300482 G300482 P01---=REVERSETRANSCRIPTASE HOMOLOG {RETROVIRAL ELEMENT); 742 5895 11041 1.3 1.OOE-124 AF155654.1 NT Human putative ribosomal protein S1 mRNA 791 5942.11093 1.1 1.OOE-124 4507500 NT Homo saplens T-cell lymphoma Invasion and metastasis 1 (TiAM1) mRNA 886 6034 11192 0.6 1.OOE-124 7705446 NT Homo sapiens hypothetical protein (HSPC068), mRNA 1324 6453 11618 2.0 1.OOE-124 AF274892.1 NT Homo sapiens glucose transporter 3 gene, exons 9, 10, and complete cds 1324 6453 11619 2.0 1.OOE-124 AF274892.1 NT Horno sapiens glucose transporter 3 gene, exons 9, 10, and complete cds 1773 6894 12082 0.9 1.0OE-124 AJ131712.1 NT Homo sapiens mRNA for nudeolar RNA-helicase (noH61 gene) 1802 6921 12107 0.9 1.0OE-124 4508032 NT Homo sapiens zinc finger protein 76 (expressed In tests) (ZNF76) mRNA 2309 7412 0.8 1.OOE-124 AW846943.1 EST HUMAN RC3-CTO11 97-150999-011 -GOS CTO1 97 Homo sapiens eDNA 2408 7507 12714 0.9 11.0OE-124 A13024069.11 NT Homo saplens gene for B 120, exon 11 3319 8464 13577 0.9 11.0OE-124 4504116 NT Homo sapiens glutamate receptor, lonotroplc, kainate 1 (GRIKI1) mRNA 3446 8588 13701 1.0 1.0OE-1124 S78684.1 NT Homo sapiens ATIP-sensitive Inwardly rectifying K-channel subunit (KCIN1J6/BIR1) gene, exon 3446 8588 13702 1.0 1.0OE-1124 S78684.11 NT Horno sapiens ATP-sensifive inwardly rectifying K-channel subunit ( 1 KCNJ6/BIR11) gene, exon 3598 8737 13832 3,0 11.0OE-124 X1 3794.1 NT H.sapiens lactate dehydrogenase B gene exon 1 and 2 (EC 1.1.1.27) (and joined CIDS) 3825 8962 14060 1.1 11.0OE-124 4507500 NT Homo sapiens T-cell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 3983 9115 14198 1.2 11.0OE-124 4504116 NT Homo saplens glutamate rece tor, ionotroplc. kainate 1 (GRIKII) mRNA 4639 9753 14847 1.6 11.0OE-1124 AB024069.1 NT Homo saplens gene for B1 20, exon 11 4850 9957 1.1 11.0OE-124 M18178.1 NT Human fibronectin gene extra type Ill repeat (EDII), exon x+l 50r -10139 15217 2,7 11.0OE-124 A1204535.1 EST HUMAN qf56h03.xl Soares testis NHT Homo sapiens cDNA done IMAGE:1754069 3' Page 160 of 196 Table 4 p CA 316 5500 1.8 1.OOE-125 A13032998.1 NT Homo sapiens mRNA for K1AA1 172 protein, partial ods 628 5787 10909 0.6 1.0OE-125 A1110656.1 EST-HUMAN HA0086 Human fetal liver cIDNA library Homo sapiens cIDNA 628 5787 10910 0.6 1.OOE-125 A1110656.1 EST-HUMAN HA0086 Human fetal liver cDNA library Homo sapiens cIDNA 711 5865 11001 1.2 1.OOE-125 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial cds 842 5991 11151 0.7 1.OOE-125 AA042813.1 ES--HUMAN zk53c07.sl Soares_pregnant uterus_NbHPU Homo sapiens cDNA done IMAGE:486540 3' similar to qb:X65857_0sl OLFACTORY RECEPTOR-LIKE PROTEIN HG1VIP07E (HUMAN); 982 6127 11282 0.5 1.OOE-125 AL163210.2 NT Homo sapiens chromosome 21 segment HS21 C01 0 1136 6272 11422 0.6 1.OOE-125 7662279 NT Homo sapiens KIAA0744 gene product: histone deacetylase 7 (K1AA0744), mRNA 1649 7922 11946 1.1 1.OOE-125 7661867 NT Homo sapiens KIAA0022 gene product (K1AA0022), mRNA 1769 6890 12077 1.4 1.OOE-125 AF015450.1 NT Homo sapiens Usurpin-alpha mRNA, complete cds 1769 6890 12078 1.4 1.OOE-125 AF015450.1 NT Homo sapiens Usurpin-alpha mRNA, complete cds 2395 7495 0.91 1.0OE-125 6005756 NT Homo saplens chromatin-specific transcription elongation factor, 140 kDa subun t (FACTP140), mRNA 2455 7554 12760 1,2 1.OOE-125 AA042813.1 EST HUMAN zk53c07.sl Soares_pregnant-uterus_NbHPU Horno sapiens cDNA clone IMAGE:486540 3' similar to gb:X65857_cdsl OLFACTORY RECEPTOR-LIKE PROTEIN HG1VIP07E (HUMAN); 2551 7646 12847 0.6 1.0OE-125 4504696 NT Horno sapiens inhibin, alpha (INHA) mRNA 2551 7646 12848 0.6 1.OOE-125 4504696 NT Homo sapiens Inhibin, alpha (INHA) rnRNA 2555 7649 12852 0,9 1.OOE-125 A1732966.1 EST HUMAN. oh64d02.x5 NCI- CGAP_K!d5 Horno saRiens cIDNA done IMAGE:1471779 3' 3089 8240 13340 1,4 1.OOE-125 P52742 SWISSPROT ZINC FINGER PROTEIN 135 3089 8240 13341 1.4 1.0OE-125 P52742 SWISSPROT ZINC FINGER PROTEIN 135 3089 8240 13342 1.4 1.OOE-125 P52742 SWISSPROT ZINC FINGER PROTEIN 135 4516 9635 14727 0.8 1.0OE-125 BE315412.1 EST-HUMAN 601141152F1 NIH MGC_91 Horno sapiens cDNA done IMAGE:3140796 5' 757 5909 11057 0.9 1.OOE-126 4758007 NT Homo sapiens CDC-like kinase (CLK) mRNA 760 5912 11060 1.2 1.OOE-126 M61936.1 NT Human laminin B1 chain gene, exon 20 845 5994 2,5 1.OOE-126 AWS78952.1 EST-HUMAN PM2-CT0328-1100100-001-h12 CT0328 Horno sapiens cIDNA 901 6048 11202 0.7 1.OOE-126 X68735.1 NT H.sapiens gene for alphal- antichymotrypsin, exon 3 2371 7473 0.7 1.0OE-126 BE258140.1 EST-HUMAN 601114764F1 NIH MGQ_16 Homo sapiens cIDNA clone IMAGE:3355622 5' 2552 7647 12849 0.8 1.OOE-126 6382078 NT Homo sapiens RAN binding protein 2 (RANBP2), mRNA 2754 7838 13039 1.8 1.OOE-126 BE275250.1 EST-HUMAN 601122073F1 N1H MGC 20 Homo saplens cIDNA done IMAGE:3346075 5' 3035 8187 13289 6.1 1.0OE-126 AA160709.1 EST HUMAN zo72c03xl Stratagene pancreas (#937208) Homo sapiens cIDNA done IMAGE:592420 5, 3035 8187 13290 6.1 1.OOE-126 AA160709.1 EST-HUMAN zo72c03,rl Stratagene pancreas (#937208) Homo sapiens cDNA clone IMAGE:592420 C 3577 8716 13813 1.2 1.OOE-126 X53941.1 NT H.sapiens DNA for liver cytochrome 155 pseudogene 3605 8744 13839 2.0 1.0OE-126 7657038 NT Horno sapiens death receptor 6 (DR6), mRNA 4677 9789 14888 1.0 1.0OE-126 AF101108.1 NT Homo sapiens collagen type XI alpha-1 (COL1 1A1) gene, exon 63 4677 9789 14889 1.0 1.OOE-126 AF101108.1 NT Homo sapiens collagen type Xl alpha-1 (COLI1A1) gene, exon 63 4728 9840 14932 1.6 1.OOE-126 N34018.1 EST_HUMAN yx78c06xl Soares melanocyte 2NbHM Homo sapiens cDNA done IMAGE:267850 5' 5360 10486 1.2 1.0OE-127 AB024597.1 NT Homo sapiens mRNA for casein kinase 1 epsilon, complete cds Page 161 of 196 Table 4

N C 5360 10487 1.2 1.OOE-127 AB024597.1 NT Homo sapiens mRNA for casein kinase 1 epsilon, comelete cds 166 5360 10486 0.8 1.OOE-127 AB024597.1 NT Homo sapiens mRNA for casein kinase 1 epsilon, complete ods 166 5360 10487 0.8 1.OOE-127 AB024597.1 NT Homo sapiens mRNA for casein kinase 1 epsilon, complete ods 272 5460 10588 0.8 1.0OE-127 087675.1 NT Homo saplens DNA for amytold precursor protein, complete cels 272 5.460 10589 0.8 1.OOE-127 D87675.1 NT Homo saplens DNA for amylold precursor protein, complete cds 863 6011 11172 1.3 1.OOE-127 AF1 14488.1 NT Homo saplens Intersectin short Isoform (ITSN) mRNA, complete ods 898 W-45 11201 1.3 1.OOE-127 U72621.2 NT Homo sapiens lost on transformation LOT1 mRNA, complete cds 1665 6789 11965 1,0 1.OOE-127 4827053 NT Homo sapiens ubiquitin specific protease 8 (USP8) mRNA 2020 7133 12332 0.6 1.OOE-127 5803065 NT Homo sapiens leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1 (LILRA1), mRNA 2020 7133 12333 0.6 1.OOE-127 5803065 NT Homo sapiens leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1 (LILRA1), mRNA 2153 7263 12471 2.7 1.OOE-127 4506620 NT Homo sapiens ribosomal protein L26 (RPL26 mRNA 2566 7659 12862 1.0 1.0012-127 4557887 NT Homo sapiens keratin 18 (KRT1 8) mRNA 2579 7672 12873 1.0 1.OOE-127 AW403790.1 EST HUMAN Ul-HF-BKO-abn-a-06-0- Ul.rl NIH-MW36 Homo sapiens cDNA clone IMAGE:3056555 T 2579 7672 12874 1.0 1.OOE-127 AW403790.1 EST HUMAN Ul-HF-BKO-abn-a-06-0- Ul.rl NIH-MGC-36 Homo sapiens cDNA done IMAGE:3056555 5' 3436 8578 13689 1.0 1.OOE-127 AW610489.1 EST HUMAN RCO-ST0379-210100-032- h12 ST0379 Homo sapiens cDNA 3436 8578 13690 1.0 1.0OE-127 AW610485.1 EST-HUMAN RCO-ST0379-210100-032h12 ST0379 Homo sapiens cIDNA 3740 8877 13967 0.9 1.0OE-127 AW161297.1 EST-HUMAN au80e06.y11 Schneider fetal brain 00004 Homo sapiens cDNA done IMAGE:2782594 S' similar to TR:Q15170 Q15170 TRANSCRIPTION FACTOR S-II-RELATED PROTEIN contains element MER22 repetitive element; 4026 9154 14238 1.1 1.0OE-127 AF135188.1 NT Homo saplens delayed rectifier potassium channel subunit IsK mRNA, complete cds 4128 9254 14334 0.9 1.OOE-127 AL163247.2 NT Homo sapiens chromosome 21 segment HS21 C047 4160 9286 14365 21,5 1.OOE-127 7706239 NT Homo sapiens neuroblastoma- amplified protein (LOC51594), mRNA 4160 9286 14366 21.5 1.OOE-127 7706239 NT Homo sapiens neuroblastoma- amplified protein (LOC51594), mRNA 4395 9517 14607 0.9 1.OOE-127 AF252297.1 NT Homo saplens cytochrome P450 refinold metabofidng protein P450RAI-2 mRNA, complete cds 4499 9619 14710 4,2 1.OOE-127 4506384 NT Homo saelens RAD1 (S. pombe) homolog (RAD1) mRNA, and translated products 4532 9651 1.9 1.OOE-127 AL163268.2 NT Homo sapiens chromosome 21 segment HS21 C068 4575 9692 14784 1.0 1.OOE-127 6912639 NT Homo sapiens Ringl and YY1 binding protein (RYBP), mRNA 452 5618 10746 1.5 1.0OE-128 BE385617.1 EST HUMAN 601278127F1 N1H MGC 20 Homo sapiens c1DNA done IMAGE:3618822 9 1138 6274 11424 1.5 1.OOE-128 4758081 NT Homo sapiens chondrolfin sulfate proteogiycan 2 (versican) (CSPG2) MRNA 1138 6274 11425 1.5 1.00EA28 4758081 NT Homo sapiens chondroldn sulfate proteoglycan 2 (versicag) (CSPG2) mRNA 2025 7138 12337 4.4 1.00EA28 U02523.1 NT Human FAU1 P pseudogene, trinuclootide repeat regions 2025 7138 12338 4.4 1.OOE-128 U02523.1 NT Human FAU1P pseudogene, trinudeodde repeat regions 2162 7271 12482 4.8 1.00EA28 4506718 NT Homo sapiens ribosomal protein S2 (RPS2) mRNA 3348 8492 1 1.00EA28 AB033073.1 NT Homo sapiens mRNA for K1AA1 247 protein, partial cds 116 5575 136! 1431l. 1.00EA29 S37722.1 NT insulin-like growth factor binding protein-2 [human, placenta, Genomic, 1019 nt, segment 2 of 107 2,2 4] Page 162 of 196 Table 4

C4- Zk 407 5575 10714 0,8 1.0012-129 S37722.1 NT Insulin-flke growth factor binding protein-2 [human, placenta, Genomic, 1019 nt, segment 2 of 4] 1689 6813 11993 1.0 1.0012-129 ALog688o.i NT Novel human mRNA containing Mnc finger C2H2 type domains 2751 7835 13035 1.2 1.OOE-129 4505682 NT Horno sapiens platelet-derived growth factor receptor, beta polypepfide (P1)GFIRB) mIRNA 2751 7835 13036 1.2 1.OOE-129 4505682 NT Homo sapiens platelet-derived growth factor receptor, beta polypepfide (PIDGFIRB) mRNA 4004 9135 14217 6.1 1.OOE-129 4504686 NT Homo sapiens IMP (Inosine monophosphate) dehyd"enase 1 (IMPDH1) mRNA 4073 9200 14286 2.2 1.OOE-129 A8040892.1 NT Homo saffiens mRNA for KIAA1459 protein, partialcds 4183 9309 14388 9.7 1.OOE-129 AW755254.1 EST-HUMAN CMYA5 Human cardiac muscle expression library Horno sapiens cDNA done 4151935 similar to CMYA5 Cardiomyopathy associated gene 5 4183 9309 14389 9.7 1.OOE-129 AW755254.1 EST-HUMAN CMYA5 Human cardiac muscle expression library Homo sapiens cDNA done 4151935 similar to CMYA5 Cardiomyopathy associated gene 5 192 5386 10513 1.0 1.00EA 30 AF233453.1 NT Homo sapiens RACK-like protein PIRKCBP1 (PRKCBPI) mIRNA,. complete cds 192 5386 10514 1.0 1.OOE-130 AF233453.1 NT Homo sapiens RACK-like protein PIRKC13P11 (PIRKCBP1) mRNA, complete cds 993 6136 0.7 1.OOE-130 AJ133269.1 NT Homo sapiens caveolin-11-2 locus, Condgl, D7S522, genes CAV2 (exons 1. 2a, and 2b), CAV1 (exons 1 and 2) 994 6136 1.2 1.OOE-130 AJ133269.1 NT Homo saplens caveolin-11-2 locus, Configl, D7S522, genes CAV2 (exons 1, 2a, and 2b), CAV1 (exons 1 and 2) 1643 6769 11940 2.5 1.OOE-130 BE275192.1 EST-HUMAN 601121995F1 N1H MGC 20 Homb saplens cONA clone IMAGE 3346366 5 1643 6769 11941 2.5 1.0012-130 BE275192.1 EST HUMAN 601121995F1 NIH MGC 20 Horno sapiens cDNA done IMAGE:3346366 5' 1940 7057 0.8 1.OOE-130 X04092.1 NT Human gene for catalase (EC 1.11.1.6) exon 9 mapping to chromosome 11, band p13 2253 7359 12566 1.5 1.0OE-130 BE280697.1 EST-HUMAN 601155971 Fl NIM- MGQ_21 Homo sapiens cDNA done IMAGE:3139408 5' 2263 7359 12567 1.5 1.OOE-130 BE280697.1 EST-HUMAN 601155971 Fl Nlimi_lVIGC 21 Homo sapiens cDNA done JMAGE:3139408 5' 2743 7827 2.8 1.00EA 30 AJ010230.1 NT Homo sapiens RET finger protein- like 1 antisense transcript, partial 2952 8103 13209 0.8 1.OOE-130 4505458 NT Homo sapiens neuropilin 2 (NIRP2) mRNA 2952 8103 13210 0,8 1.OOE-130 4505458 NT Homo sapiens neuropilin 2 (NIRP2) mRNA 3530 8671 13775 1.1 1.0OE-130 AF240698.1 NT Homo saplens refinol dehydrogenase homolog isoform-l (RDH) mIRNA, complete cds 3857 8993 14092 1.1 1.OOE-130 AW503580.1 EST-HUMAN Ul-HF-BNO-aky-g-O"-Ul. rl NIH-MGCL50 Horno saplens cIDNA done IMAGE:3078731 5' 4438 9559 14654 7.5 1.0OE-130 AW843993.1 EST-HUMAN CM4-CN0045-180200-51 1402 CN0045 Homo sapiens cIDNA 5029 10130 15205 1.1 1.OOE-130 AW363299.1 EST-HUMAN RCO-CTO318-201199-031 -all CT0318 Homo sapiens cIDNA 5029 10130 15206 1.1 1.0OE-130 AW363299.1 EST-HUMAN RCO-CTO318-201 19M31- al 1 CT0318 Homo sapiens cDNA 4 5213 10314 0.9 0 AA228126.1 EST HUMAN zr58c04.rl Soares_Nhi-IMPu-S1 Homo sapiens cDNA done IMAGE:667590 5'similar to TR:G222811 G222811 ALPHA 1 CHAIN OF TYPE XII COLLAGEN.; 4 5213 10315 0.9 0 AA228126.1 EST-HUMAN zr58c04xl Soares-NhHIVIP4_S1 Horno saplens c1DNA done IMAGE:667590 5' similar to TR:G222811 G222811 ALPHA 1 CHAIN OF TYPE XII COLLAGEN.; 7 5215 10318 1.4 0 4885136 NT Homo sapiens checkpoint suppressor 1 (CHES1), mRNA 522 10324 1.3 0 8923349 NT Homo sapiens hypothetical protein FLJ20371 (FU20371), mRNA 5223 1.3 0 8923349 NT Homo sapiens hypothetical protein FLJ20371 (FL. 120371), mIRNA L___,5229 1 2.5 0 D83327.1 NT Homo sapiens OCIRR1 mRNA, partial cds Page 163 of 196 Table 4 t- 14,11 4 5229 10332 2.5 0 D83327.1 NT Horno sapiens DWR1 mRbJk partial cds 24 5233 10336 1.9 0 AF141349.1 NT Homo sapiens beta-tubulin mRNA, complete ods 33 5242 10348 1,0 0 M58600.1 NT Human heparin cofactor 11 (HCF2) gene, exons-1 th U h 5 5244 10351 0.8 0 6857825 NT Homo saplens RNA-binding protein SI, sedne- rich domaifi (RNPS1), mRNA 51 5260 10377 1.4 0 Y17151.2 NT Horno sapiens mRNA for mulfidrug resistance protein 3 (ABCC3) 51 5260 10378 1.4 0 Y17151.2 NT Homo sapiens mRNA for multdrug resistance proteln_3 (ABCC3) 52 5261 10379 1.2 0 D78804.1 EST HUMAN HUM516HO8B Human placenta polyA+ (TFujiwara) Horno, sapiens cIDNA, clone GEN-516H08 5' 52 5261 10380 1.2 0 D78804.1 EST-HUMAN HUM516HO8B Human placenta polyA+ (TFujiwara) Homo sapiens cIDNA clone GEN-516H08 S' 53 5262 10381 1.4 0 L16558.1 NT Human dbosonial protein L7 (RPL7) mRNA, comp ete s 5264 10384 2.8 0 AW069534.1 EST HUMAN cr48e07.xl Jia bone marrow strorna Homo sapiens cDNA done I-IBIVISC cr48e07 3' 5264 10385 2.8 0 AW069534.1 EST HUMAN cr48e07.xl Jia bone marrow stroma Horno s;piens cDNA clone HBIVISC cr48e07 3' 59 5267 10389 2.0 0 M60676.1 NT Human von Willebrand factor pseudogene corresponding to exons 23 through 34 61 0.9 0 M60676.1 NT Human von Willebrand factor pseudogene corresponding to exons 23 through 34 69 51269 10401 0.6 0 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrata 1 (PTPNS1) mRNA 5276 69 5276 10402 0.6 0 4758977 NT Homo sapiens protein tyrosine Phosphatase, non-receptor type substrate 1 (IDTPNS1) mRNA 71 5276 10401 0.5 0 4758977 NT Homo sapiens protein. tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 71 5276 10402 0.5 0 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 74 5280 10407 14.0 0 AA953770.1 EST-HUMAN on89e04.sli Soares - NFLj_GBC - S1 Horno sapiens cIDNA done IMAGE:1563870 3'similar to SW:TMOP_HUhiAN P28289 TROPOMODULIN.; 76 5282 10409 1.1 0 4501850 NT Homo sapiens amiloride binding protein 1 (amine oyJdase (copper-wntaining)) (A13P1), nuclear gene encoding mitochondrial protein, mRNA 77 5283 5.0 0 4504444 NT Homo sapiens heterogeneous nuclear dbonucleoproteln A1 (HNRPA1) m 1 RNA 86 5292 10418 16.0 0 5016088 NT Homo sapiens actin, beta (ACTB) mRNA 89 5295 10421 4.6 0 U89277.1 NT Human polyhomeofic 1 homolog (HPHI) mRNA, partial ods 94 5301 10427 1.5 0 A1114743.1 EST HUMAN HA1 347 Human fetal liver cDNA library Horno sapiens cDNA 5302 10428 1.0 0 AB037784.1 NT Homo sapiens mRNA for KIAA1363 protein, partial cds 102 50-7 10433 2.1 0 X91213.1 NT H.sapiens ncxl gene (exon 2) 5313 10438 1.4 0 A1623701.1 EST HUMAN ts38b05.xl NCI-CGAP-Ut4 Homo sapiens cDNA clone IMAGE:2230833 Ysimilar to TR:Q99551 Q99551 MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR PRECURSOR.; 111 5313 10438 0.6 0 A1623701.1 EST-HUMAN ts38bO NCI-CGAP-Ut4 Homo saplens cIDNA clone IMAGE:2230833 Ysimilar to TR:099551 Q99551 MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR PRECURSOR.; 112 7858 10439 0.6 0 N36040.1 EST HUMAN yy01h09xl Soares melanocyte 2NbHM Horno sapiens cDNA clone IMAGE:270017 5' Page 164 of 196 Table 4

V\ 1p 9 5316 10445 0.9 0 4505458 NT Homo saplens neuropilin 2 (NRP2) mRNA 126 5322 10453 1.1 0 4505938 NT Homo sapiens polymerase (RNA) 11 (DNA directed) polypeptide A (220kD) (POLR2A) mRNA 126 5322 10454 1.1 0 4505938 NT Homo sapiens polymerase (RNA) 11 (DNA directed) polypeptide A (220kD) (POLR2A) mRNA 5330 10461 0.5 0 T56945.1 EST HUMAN ya83g04x2 Stratagene fetal spleen (#937205) Homo sapiens cDNA clone IMAGE:68310 5' 5330 10462 0.5 0 T56945.1 EST HUMAN ya83904.r2 Stratagene fetal spleen (#937205) Homo sapiens cDNA done IMAGE:68310 5' 147 5343 3.1 0 4504444 NT Homo saple s heterogeneous nuclear dbonudeoproteln AI (HNIRPA1) mRNA 153 5349 5.4 0 4504444 NT Horno sapiens heterogeneous nuclear dbonudeoproteln A1 (HNRPA1) mRNA 156 5352 10478 1.4 0 AF1 11168,2 NT Homo sapiens serine palmitoyl transferase, subunit 11 gene, complete ods; and unknown genes 158 5354 10479 1.1 0 BE295973.1 EST HUMAN 601174270F1 N1H MGC 17 Honio sapiens cDNA done IMAGE.3529864 5' 159 5354 10479 1.2 0 BE295973.1 EST-HUMAN 601174270F1 N1H MGC 17 Homo sapiens cDNA done IMAGE:3529864 5' 5355 10480 1.0 0 W73973.1 EST-HUMAN zd62b05.rl Soaresjetal_ heart NbHH1 9W Homo sapiens cDNA done IMAGE:345201 S' similar to gb:X16282 odsl ZINC FINGER PROTEIN CLONE 647 (HUMAN); 161 5356 10481 0.5 0 AF244088.1 NT Homo sapiens Anc finger protein mRNA, complete cds 164 5359 10484 6.3 0 AIL163202.2 NT Homo saplens chromosome 21 segment HS21 C002 164 5359 10485 6.3 0 AL163202.2 NT Horno sapiens chromosome 21 segment HS21 C002 174 5367 10492 1.5 0 BE018970.1 EST-HUMAN bb24e12.yl N1H MGC 14 Horno sapiens cDNA done IMAGE:253854 5'similar to WP:Y57A10A.Z7E22j31; 174 5367 10493 1,5 0 BE018970.1 EST-HUMAN bb24e12.yl NIH-MGC-14 Horno saplens cD.NA done IMAGE:2963854 5'similar to WP:Y57A10A.ZCE22631; 179 5372 10496 0.7 0 A13018327.1 NT Homo sapiens mRNA for KIAA0784 protein partial cds 179 5372 10497 0.7 0 A13018327.1 NT Horno sapiens mRNA for KIAA0784 protein, partial cds 187 5381 10508 44.0 0 D50659.1 NT Human gamma-cytoplasmic actin (ACTGP9) pseudogene 194 5388 10516 1.0 0 AF167174.1 NT Homo sapiens chromosome X IVISI-3-2 protein mRNA, complete cds 194 5388 10517 1.0 0 AF167174.1 NT Horno sapiens chromosome X MS1-3-2 protein mRNA, complete cds 203 7883 10523 3.2 0 A1587308.1 EST HUMAN tq0008.xl NCI - CGAP W Horno sapiens cDNA done IMAGE:2207847 Ysimilar to gbA03191 PROFILIN 1 (HUMAN); 203 7883 10524 3.2 0 A1587308.1 EST-HUMAN tq04108.xl NCI-CGAP-Ut3 Homo sapiens cDNA done IMAGE:2207847 3' similar to gb:J03191 PROFILIN 1 (HUMAN); 205 5398 10526 0.7 0 AF195658A NT Horno sapiens DNA mismatch repair protein (M1-H3) gene, complete cds 208 5401 5.7 0 4506632 NT Homo sapiens dbosomal protein L31 (RPL31) mRNA 209 5402 1.2 0 AF132000.11 NT Homo sapiens TADA1 protein mRNA, complete cds 215 5408 10531 0.9 0 AB018264.1 NT Horno sapiens mRNA for KIAA0721 protein, partial cds 216 5468 10531 0.7 0 AB0118264.1 NT Homo sapiens mRNA for KIAA0721 protein, partial ods 21 5409 10532 0.6 0 6678444 NT Mus musculus tesds-specific proteirf, Y- encoded-like (Tspyl), mRNA 5417 10536 1.2 0 BE246780.1 EST HUMAN TWAP1 E4466 Pediatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project--TC13A Horno sapiens cDNA done TCBAP4466 Page 165 of 196 Table 4

224 5417 10537 1.2 0 BE246780.1 EST-HUMAN MBAP1 E4466 Pedlatric pre-B cell acute lymphoblastic leukernia Bayfor-HGSC project--TCBA Homo sapiens eDNA clone TCBAP4466 224 5417 10538 1.2 0 BE246780.1 ESTHUMAN TCBAP1E4466 Pediatric pre-B cell acute lymphoblastic leukem(a Baylor-HGSC project--TCBA Horno saplens eDNA clone TCBAP4466 225 5417 10536 1.4 0 BE246780.1 EST HUMAN MBAP1 E4466 Pedlatric; pre-B cell acute lymphoblastic leukernia Baylor-HGSC project--TCBA Homo sapiens cDNA done TCBAP4466 225 5417 10537 1.4 0 BE246780.1 EST HUMAN TWAP1 E4466 Pedlatric pre-8 cell acute lymphoblastic leukernia Bayior-HGSC project=TCBA Horno saplens cDNA clone TWAP4466 225 5417 10538 1.4 0 BE246780.1 EST_HUMAN TWAP1 E4466 Pedlatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project--TCBA Homo sapiens cDNA done TCBAP4466 226 5417 10536 4.3 0 BE246780.1 EST_HUMAN TC13AP1 E4466 Pediatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project=TCBA Homo sapiens cDNA done TCBAP4466 226 5417 10537 4.3 0 BE246780.1 EST-HUMAN MBAP1 E4466 Pediatric pre-B cell acute lymphoblastic leukernia Baylor-HGSC project--TCBA Homo sapiens cDNA clone TCBAP4466 226 5417 10538 4,3 0 BE246780.1 EST-HUMAN TC13AP1 E4466 Pedlatric pre-B cell acute lymphoblastic leukernia Bayfor-HGSC project=TCBA Homo sapiens cDNA clone TCBAP4466 238 5428 10555 1.6 0 5453805 NT Homo.sapiens NSI-associated protein 1 JNSAP1) mRNA 240 5430 2.2 0 AL163201.2 NT Homo sapJens chromosome 21 segment HS21C001 247 5435 10561 1.3 0 AF231 919.1 NT Homo saplens chromosome 21 unknown mRNA 249 5437 10564 1.5 0 X89772.1 NT H.sapiens mRNA for Interferon alphalbeta receptor (long form) 257 5445 2.3 0 AF231919.1 NT Horno sapiens chromosome 21 unknown mRNA 269 5456 10582 1.1 0 4507500 NT Homo sapiens T-cell lymphorna Invasion and metastasis 1 (TIAM1) mRNA 269 5456 10583 1.1 0 4507500 NT Homo sapiens T-cell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 270 5458 10585 0.9 0 7706028 NT Homo sapiens hypothetical protein (LOC51250), mRNA 281 5468 10598 1.1 0 D83327.1 NT Homo saplens DCRR1 mRNA, partial cds.

281 5468 10599 1.1 0 D83327.1 NT Horno sapiens DCRR1 mRNA,_pardat cds 282 5469 0.9 0 AW845293.1 EST-HUMAN IL2-CTO031-181199-020-803 CTO031 Homo sapiens cDNA 290 5476 10606 1.8 0 4557029 NT Homo sapiens potassium inwardly- rectifying channel, subfamily J, member 15 (KCNil 5) mRNA 290 5476 10607 1.8 0 4557029 NT Homo sapiens potassium inwardly- rectifying channel, subfamily J, member 15 (KCNJ15) mRNA 301 5487 10617 1.4 0 AB028942.1 NT Homo sapiens mIRNA for KIAA1019 protein. partial cds 302 5488 10618 1.1 0 AB028942.1 NT Homo sapiens mIRNA for KJAM 019 protein, partial cds 303 7886 1.8 0 4506728 NT Horno sapiens ribosomal protein S5 (RPS5) mRNA 304 5489 10619 0.7 0 4503914 NT Homo sapiens phosphodbwylgiycinamide formtransferase, phosphodbosAglycJnamide synthetase, phosphodbosylaminolmidazole synthetase (GMT) MRNA 305 5490 0.7 0 AA480002.1 EST-HUMAN M M6.rl Soares Nhi-IMPuAl Homo sapiens cDNA done IMAGE:753994 9 306 5491 10620 3.3 0 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 307 5491 1062 4. 0 4507152 NT Horno sapiens SON DNA binding protein (SON) mRNA 31 t --495 10624 0.8 0 AF114488.1 NT Horno sapiens Intersectin short Isoform (ITSN) mRNA, complete ods Page 166 of 196 Table 4

324 5507 10633 0.9 0 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACHI (BTB AND MC HOMOLOG 1) (HA2303) 324 5507 10634 0.9 0 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND MC HOMOLOG 1) (HA2303) ' 325 5508 10635 1.4 0 7657213 NT Homo sapiens hormonally upregulated neu turnor-assocated kinase (HUNK), rnRNA 326 5508 10635 0.8 0 7657213 NT Homo sapiens hormonally upregulated neu tumor-assocJated kinase (HUNK), m NA 341 5522 10647 1.3 0 5174574 NT Homo, sapiens myelold/lymphold or mixed- lineage leukernia (trithorax (Drosophila) hornolog); translocated to, 4 (MLLT4) mRNA 342 5523 10648 0.9 0 4505256 NT Homo sapiens moesin (MSN), mRNA 345 5526 10652 1,3 0 4827057 NT Homo sapiens X-box binding protein 1 (X13P1) mRNA 348 5529 10657 0,8 0 U71600.1 NT Human zinc finger protein zfp31 (M1) mRNA, partial cds 353 5533 10661 0.7 0 AF231919.1 NT Homo sapiens chromosome 21 unknown mRNA 353 5633 10662 0.7 0 AF231 919.1 NT Homo sapiens chromosome 21 unknown mRNA 7887 10663 1.2 0 AF231 919.1 NT Homo sapiens chromosome 21 unknown mRNA 356 5535 10665 0.9 0 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 358 5537 10668 1.4 0 4503854 NT Homo saplens GA-binding protein transcripdon factor. alpha subunit (60kD) (GA13PA), mRNA 359 5538 10669 1.4 0 D80006.1 NT Human mRNA for KIAA0184 gene, partial ods 360 5638 10669 0.5 0 D80006.1 NT Human mRNA for KIAA0184 gene, partial ods 381 5587 10720 1.8 0 A13028942.1 NT Homo saplens mRNA for K1AA1019'protein, partial cds 382 5588.10721 1.0 0 A1363014.1 EST-HUMAN qy81h05.xl NCI_CGAP_Bm25 Homo sapiens cDNA cJo IMAGE:2018457 Ysimilar to gb:X54199 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (HUMAN); 387 5556 10688 3.4 0 AW754180,1 EST HUMAN IRC2-CT0320-300100-016-a09 CT0320 Horno sapiens cDNA 390 5558 10691 1.4 0 4503680 NT Homo sapiens I9G Fc binding protein (FC(GAMMA)BP) mRNA 391 5559 10692 0.7 0 4503680 NT Homo, sapiens igG Fc binding protein (FC(GAMMA)13P) mRNA 391 5559 10693 0.7 0 4503680 NT Homo saplens IgG Fc binding protein (FC(GAMMA)1312) mRNA 392 5560 10694 1.2 0 4503680 NT Homo sapiens 19G Fe binding Rrotein (FC(GAMMA)11P) mRNA 393 5561 10695 0.6 0 4503680 NT Homo saplens IgG Fc binding protein (FC(GAMMA)BP) mRNA 393 5561 10696 0.6 0 4503680 NT Homo saplens IgG Fc binding protein (FC(GAMMA)W) mRNA 394 5562 10697 2.4 0 4503680 NT Horno sapiens IgG Fc binding protein (FC(GAMMA)B12) MRNA 395 5563 10698 0.9 0 4503680 NT Homo sapiens IgG Fc binding protein (FC(GAMMA)BP) mRNA 396 5564 10699 0.8 0 X74870.1 NT H.sapiens gene for RNA pol 11 largest subunit, exons 23-29 396 5564 10700 0.8 0 X74870.1 NT H.sapiens gene for RNA pol 11 largest subunit, exons 23-29 400 5568 15.0 0 4506608 NT Homo saplens ribosomal protein L19 (RPLI9) rnRNA 414 5203 10304 1.3 0 R17795.1 EST-HUMAN yg09a02.M Soares Infant brain 1 NIB Homo sapiens cDNA done IMAGE:31652 5, 422 5589 0.9 0 4506728 NT Horno, sapiens dbosornal protein S5 (RPS5) mRNA 423 5590 10722 0.8 0 A13028942.1 NT Homo sapiens mRNAfor KIAA1019 protein, partial cds 424 5591 10723 1,6 0 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 424 5591 10724 1.6 0 4507152 NT Horno sapiens SON DNA binding protein (SON) mRNA 425 5592 10725 1.2 0 AF1936077 NT Mus musculus truncated SON úroteln (Son) mRNA, complete ods 4557879 NT Homo sapiens interferon gamma receptor 1 (IFNGR1) mRNA Page 167 of 196 Table 4 p lp c 443 5609 1.0 0 AA324262.11 EST HUMAN EST27054 Cerebellum 11 Homo sapiens cDNA 6 end 444 5610 0.9 0 BE254447.1 EST HUMAN 601111520F1 N1H MGC 16 Homo sapiens cDNA done (MAGE,3352348 5 460 5626 10752 1,1 0 4504532 NT Homo sapiens 5-hydroxytfyptamine (serotonin) receptor 1 S (HTRI B) mRNA 460 5626 10753 1.1 0 4504532 NT Horno sapiens 5-hydnqxffiptamine (serotonin) receptor 1 (HTIR1 B) mIRNA 465 5830 10760 1.3 0 4557887 NT Homo saplens keratin 18 (KIRT18) mRNA 465 5630 10761 1.3 0 4557887 NT Homo sapiens keratin 18 (KIRT18) mRNA 475 5641 10767 0.8 0 AL163246.2 NT Homo sapiens chromosome 21 segment HS21C046 476 56.42 10768 2.4 0 AL163246.2 NT Homo sapiens chromosome 21 segment HS21 C046 476 5642 10769 2.4 0 AIL163246.2 NT Homo sapiens chromosome 21 segment HS21C046 485 5650 10774 0.9 0 A13033035.1 NT Horno sapiens_mRNA for KIAA1209 protein, partial cds 495 5660 10782 0.7 0 BE385144.1 EST-HUMAN 601274951F1 NIM_MGP_20 Homo sapiens cDNA done IMAGE:3615756 5, 496 7890 10783 1.1 0 AW938825.1 EST-HUMAN PMO-DT0065-130400-002-c06 DT0065 Homo sapiens cDNA 498 5662 10785 1.1 0 AL1 17233.1 NT Novel human gene mapping to chomosome 1 499 5663 10786 0.6 0 8923955 NT Homo sapiens PC326 protein (PC326), mRNA 508 5672 10795 1.3 0 A1-163210.2 NT Homo saplens chromosome 21 segment HS21C010 515 7891 10799 0.7 0 BE081527,1 EST HUMAN QV2-13TO635-160400-1142-h05 BT0635 Homo sapiens cDNA 526 5690 10812 1.2 0 A13040909.1 NT Homo sapiens mRNA for KIAA1476 protein, partial ccis 529 5693 10815 3,8 0 6006030 NT Homo sapiens transcription elongation tactor B (Sill), polypepfide 1-like (TCEB1 Q mIRNA 530 5694 10816 1.4 0 4504036 NT Homo saplens guanine nudeotide binding protein (G protein), alpha 11 (Gq dass) (GNA1 1) mRNA 530 5694 10817 1.4 0 4504036 NT Homo saplens quanine nudeotide binding protein (G protein), alpha 11 (Gq class) (GNA1 1) mIRNA 536 5699 2.0 0 AF003528.1 NT Homo sapiens X-linked anhiciroitic ectoderrnal dysplasia protein gene (EDA), exon 2 and flanking repeat regions 544 5707 10827 0.6 0 AW135324.1 EST-HUMAN Ul-H-Bil-acb-b-04-0-Ul.sl NCI- CGAP_Sub3 Homo saplens cDNA done IMAGE:2713951 3, 554 5717 1.1 0 D10083.1 NT Homo sapiens RGH1 gene, retrovirus-like element 571 5732 10850 0,9 0 5174742 NT Homo sapiens ubiquinot-cytochrome c red. uctase, Rieske Iron-sulfur polypepfide 1 (UQCRFSI), nuclear gene encoding mitochonddal protein, mRNA 584 5744 1.8 0 J04066.1 NT Human apollpoprotein A-1 (ApoA-1) gene, exon 1 593 5751 10868 1.5 0 4501854 NT Homo sapiens acetyl-Coenzyme A carboxylase beta ACB), mRNA 598 5756 10874 1.1 0 AF221712.1 NT Horno sapiens Smad- a - nd Olfinteracting zinc finger protein mRNA, partial cds 598 5756 10875 1.1 0 AF221712.1 NT Homo saplens Smad- and Olf-interacting 7Jnc finger protein mRNA, partial ccis 607 5765 10883 1.4 0 AF149773.1 NT Homo sapiens NOD1 protein (NOD1) gene, exons 1, 2, and 3 610 5768 10886 1.0 0 6806918 NT Homo saplens low density lipoprotein- related protein 2 (LRP2), mRNA 611 5769 10887 0.8 0 6806918 NT Homo sapiens low densi!y lipoprotoin- telated protein 2 (LRP2), mRNA 611 5769 10888 0.8 0 6806918 NT Homo sapiens low density lipoprotein- retated protein 2 (LR122), mIRNA 612 5770 10889 0.9 0 6806918 NT Homo saplens low density lipoprotein- related protein 2 (LRP2), mIRNA 612 5770 10890 0.9 0 6806918 NT Homo saplens low density lipoprotein- retated protein 2 (LRP2), mIRNA 5778 10900 1.3 0 AA3M86.1 EST HUMAN zt60c07.rl Soares testis NHT Homo saplens cDNA done IMAGE:726732 S' Page 168 of 196 Table 4

Z_. n r 623 5782 10904 2.2 0 D1 1078.1 NT Homo sapiens RGH2 gene, retrovirus-like element 627 5786 10907 1.1 0 W7881 1.1 EST HUMAN zh51 b04.rl Soares_fetaLliveLspicerLl NFI.SSli Homo sapiens cDNA clone IMAGE:415567 5'sImilar to gb.A21187 ALPHA-2-MACROGLOBULIN PRECURSOR (HUMAN); 627 5786 10908 1.1 0 W7881 1.1 EST HUMAN zh51b04xl Soares_feta, !_liver_spleenl INIFI-S-S1 Homo sapiens cDNA done IMAGE:415567 5'sImilar to gb:A21187 ALPHA-2-MACROGLOBULIN PRECURSOR (HUMAN); 630 5789 1.1 0 4885526 NT Homo sapiens novel SH2-containing protein 3 (NSP3) mRNA 637 5796 10920 1.0 0 6006003 NT Homo sapiens glutamate receptor, lonotropic, N-methyl D-aspartate 213 (GRIN2B) mRNA 639 5798 10923 1.1 0 5031624 NT Homo sapiens CCAAT-box-binding transcription factor (C13F2) mRNA 642 5801 10927 1.4 0 U05235,11 NT Human neutral amino add transporter (ASCT1) gene, exon 8 646 5805 10930 0.7 0 AF108389.1 NT Homo sapiens sodiun/caidum exchanger isoform NaCa3 (NCX1) mRNA, complete cds 646 5805 10931 0.7 0 AF1 08389.1 NT Homo sapiens sodium/calcium exchanger Isoform NaCa3 (NCX1) mRNA, complete cds 652 5810 10936 1.4 0 4826947 NT Homo sapiens protein kinase, X-linked (PRKX) mRNA 652 5810 10937 1.4 0 4826947 NT Homo sapiens protein kinase, X-linked (PRKX) mRNA 658 7894 1.0 0 X57147.1 NT Human endogenous retrovirus pHE.1 (ERV9) 667 5823 10951 1.6 0 4504424 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 1 (HMGI) mRNA 672 5828 10955 1.5 0 AB029012.1 NT Homo sapiens mRNA for KIAA1089 protein, partial cds 682 5837 10970 0.7 0 7657468 NT Homo sapiens similar to rat Integral membrane glycoprotein POM121 (POM1211.1), mRNA 692 5847 10982 6.6 0 AA614537.1 EST HUMAN rip49d01.sl NCI-CGAP-Brl.l Homo saplens cDNA done IMAGE:1 129633 Ysimilar to gb:X57352 INTERFERON-INDUCIBLE PROTEIN 1-8U (HUMAN); 696 5851 10986 26 0 M60675.11 NT Human von Willebrand factor gene, exons 23 through 34 696 5851 10987 2.6 0 M60675.11 NT Human von Willebrand factor gene,'exons 23 through 34 706 5861 10996 1.5 0 5032192 NT Homo sapiens TNF receptor-associated factor 1 (TRAF1) mRNA 712 5866 11002 1.3 0 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial cds 712 11003 1.3 0 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial ods 719 5873 11013 0,6 0 BE241577.1 EST HUMAN TCAAP1D0779 Pedlatric acute myclogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA Homo sapiens cDNA done TCAAP0779 739 5892 11037 1.1 0 AF226990.2 NT Homo sapiens MI-IC class 1 antigen (HLA-G) mRNA, HLA-Gl allele, complete cels 739 5892 11038 1.1 0 AF226990.2 NT Homo sapiens MI-IC class 1 antigen (HILA-G) mRNA, HILA-Gli allele, complete cels 740 5893 11039 2.4 0 AF170492.1 NT Homo sapiens chloride channel CLC4 (C1C4) mRNA, complete cds 743 5896 11042 0.5 0 J03764.1 NT Human, plasminogen activator inhibitor-1. gene, exons 2 to 9 743 5896 11043 0.5 0 J03764.11 NT Human, plasminogen activator inhibitor- 1 gene, exons 2 to 9 745 5898 11044 0.8 0 A13037760.1 NT Homo saplens mRNA for KIAA1339 protein, partial ods 746 5899 11045 1.1 0 6912749 NT Homo saplens zinc finger protein 212 (ZNF212), mRNA 747 7898 11046 0.8 0 D30612.1 NT Homo sapiens mRNA for repressor protein, partial cds 752 5904 11051 1.2 0 R48915.11 EST HUMAN A69g08xl Soares breast 2NbHBst Homo saplens cDNA clone IMAGE:1 54046 5' 753 5905 11052 0.8 0 5032086 NT Homo sapiens splicing factor 3a, subunit 1, 120k13 (SF3A1), mRNA 762 5914 11061 0.5 0 AB01 1399.1 NT Homo sapiens gene for AF-6. complete cels -_ 91 8 11065 1.0 0 7661965 NT Homo saplens K1AA011 70 gene product (K1AA01 70), mRNA Page 169 of 196 Table 4

775 5927 11076 1.2 0 D80006.1 NT Human mRNA for KIAA0184 gene, partial cds 775 5927 11077 1.2 0 D80006.1 NT Human mRNA for KIAA01 84 gene, partial cds 780 5932 11081 0.9 0 X89772.1 NT H.sapiens mRNA for Interferon alphalbeta receptor (long form) 784 5936 11085 0,8 0 AB020717.1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 784 5936 11086 0.8 0 A13020717.1 NT Homo sapiens mRNA for KIAA091 0 protein, pardal cds 789 5940 11092 2.3 0 5174478 NT Homo sapiens pericentrin (PCNT) mRNA 790 5941 2,4 0 4507500 NT Homo sapiens T-oell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 807 5958 11113 0.5 0 7657213 NT Homo sapiens hormonally upregulated neu tumor-assodated kinase (HUNK), mRNA 808 5959 11114 1.5 0 7657213 NT Homo saplens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 810 5961 11116 1.3 0 4557686 NT Homo sapiens potassium voitag"ated channel, Isk-related family, member 1 (KCNE11) mRNA 816 5966 11122 1.2 0 AF108830.1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 816 5966 11123 1.2 0 AF108830.1 NT Homo sapiens sedne-threonine protein kinase (MNBH) mRNA, complete eds 821 5971 11128 1.1 0 4503854 NT Homo sapiens GA-binding protein transcription factor, alpha subunit (60kD) (GABPA). mRNA 825 5974 11133 0.5 0 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 825 5974 11134 0.5 0 4507500 NT Homo sapiens T-cell lymphoma Invasion and metastasis 1 (TIAM1) mRNA 832 5981 0.5 0 AF027153.1 NT Homo sapiens sodium/myo-inositot cotransporter (SLC5A3) gene, complete cds 836 5985 11145 1.2 0 AB028942.1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 836 5985 11146 1.2 0 AB028942.1 NT Homo sapiens mRNA for K1AA11 019 protein, partial cds 837 5986 11147 2.5 0 4507152 NT Horno sapiens SON DNA binding protein (SON) mRNA 838 5987 11148 1.0 0 AB028942.1 NT Horno sapiens mRNA for KITA1019 protein, partial cds 839 5988 11149 0.7 0 4506728 NT Homo sapiens dbosomal protein SS (RPS5) mIRNA 843 5992 11152 1.3 0 A13020717.1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 843 5992 11153 1.3 0 A13020717.1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 844 5993 11154 0.7 0 AA533272.1 EST HUMAN nj66d07.sl NCI CGAP Pr10 Homo sapiens cDNA done IMAGE:997453 844 5993 11155 0.7 0 AA533272.1 EST HUMAN nj66d07.sl NCI CGAP Pr10 Homo sapiens cIDNA done IMAGE:997453 849 5998 11156 1.3 0 7657213 NT Homo sapiens hormonally upregulated neu turnor-associated kinase (HUNK), mRNA 849 5998 11157 1,3 0 7657213 NT Homo sapiens hormonally upregulated neu. turnor-assodated kinase (HUNK), mRNA 850 5999 11158 0.7 0 7657213 NT Homo sapiens hormonally upregulated neu tumor-assodated kinase (HUNK), mRNA 850 5999 11159 0.7 0, 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 873 6021 11182 0.9 0 AL1 63203.2 NT Homo sapiens chromosome 21 segment HS21 C003 880 6628 11187 0.6 0 BE089592.1 EST-HUMAN OVO-BT0703-280400-21 1 -911 BT0703 Horno sapiens cDNA 880 6028 11188 0.6 0 BE089592.1 ESTHUMAN OVO-BT0703-280400-21 1 -911 BT0703 Homo sapiens cDNA 890 6038 11197 1.3 0 AL163203.2 NT Homo sapiens chromosome 21 segment HS21 C003 900 6047 2.5 F- 4504958 NT Homo sapiens laminin recept;r 1 (67kD, dbosomal protein SA) (LAMR1), mRNA 903 6047 1.1 0 4504958 NT Homo sapiens faminin receptor 1 (67kD, dbosomal rotein SA) (LA R1), mIRNA 11205 0.5 0 AF089747.1 NT Homo saplens alpha-l-andchymotrypsin precursor, mRNPJ, partial cds 905 6051 11206 1.0 0 S69364.1 NT protein C inhibitor [human, laukoeytes, Genomic, 1216 nt, segment 2 of 5] A9046050 g0_ 6051 11207 1.0 0 S69364.1 NT rotein C inhibitor [human, leukocytes, Genornic, 1216 nt, segment 2 of 51 9 6051 11208 1.0 0 S69364.1 NT nhibitor [human, leukocytes, Genomic, 1216 nt, segment 2 of 51 Page 170 of 196 Table 4

906 6052 11209 0.7 0 L28101.1 NT Horno sapiens kallistatn (P14) gene, exons 1-4, complete cds 909 6055 11212 36.0 0 Z20656.11 NT Homo sapiens of cardiac alpha-myosin heavy chain gene 909 6055 11213 36.0 0 Z20656.11 NT Homo sapiens of cardiac alpha-myosin heavy chain gene 910 6056 11214 57.0 0 Z20656.11 NT Homo sapiens of cardiac alpha-myosin heavy chain gene 910 6056 11215 57.0 0 Z20656.11 NT Homo sapiens of cardiac alpha-myosin heavy chain gene 935 6082 11238 12.0 0 M37190.11 NT Human ras Inhibitor mRNA, Xend 936 6083 11239 4.5 0 M371 90.1 NT Human ras Inhibitor mRNA, Xend 937 6084 11240 18.0 0 M37190.1 NT Human ras Inhibitor mRNA, Yend 938 6065 11241 0.7 0 4507430 NT Homo sapiens thyrotrophic embryonic factor (TEF), mRNA 938 6085 11242 0.7 0 4507430 NT Homo sapiens thyrotrophic embronic factor (TEF), mRNA 946 7903 11249 0.8 0 A1001948.1 EST HUMAN os98c03.sl NCI CGAP GC3 Homo sapiens cDNA done iMAGE:1613404 3' 946 7903 11250 0.8 0 A1001948.1 EST HUMAN os98e03.sl NCI CGAP GC3 Homo sapiens cDNA done IMAGE:1613404 X 948 6094 2.5 0 7657266 NT Homo saplens KIAA0929 protein Msx2 Interacting nuclear target (MINT) homolog (K1AA0929), mRNA 968 6113 11271 1.3 0 X52207.1 NT Homo saplens partial c-fgr gene, exons 2 and 3 968 6113 11272 1.3 0 X52207.1 NT Homo sapiens partial c4gr gene, exons 2 and 3 977 6122 11279 1.3 0 4757969 NT Homo sapi ns chromodomain protein, Y chromosome-like (CDYL) mRNA 988 6132 11287 1.1 0 U83668.1 NT Human beta-tubulin (TUa4q) gene,. complete cds 989 6133 11288 2.6 0 U83668.11 NT Human beta-tubulin (TUB4q) gene, complete cds 990 6133 11288 2.3 0 U83668.1 NT Human beta-tubulin (TUB4q) gene, complete cds 997 6139 11293 0.8 0 AF1 11170.3 NT Homo sapiens 14g32 Jagged12 gene, complete cds; and unknown gene 998 6139 11293 1.4 0 AF111170.3 NT Homo sapiens 14g32Jagged12 gene, complete cds; and Onknown gene 999 6139 11293 0.6 0 AF111170.3 NT Homo sapiens 14g32 Jagged2 gene, complete cds; and unknown gene 1000 6140 11294 0.8 0 AF111170.3 NT Homo sapiens 14g32 Jagged2 gene, complete ods; and unknown gene 1003 6143 11297 0.8 0 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 1007 6147 11301 0.9 0 5803114 NT Homo sapiens Inner membrane protein, mitochondrial (mitofilin) (IMMT), mRNA 1009 6149 0,7 0 AA458680.1 EST-HUMAN aa86g07.sl Stratagene fetal refina 937202 Homo sapiens cDNA clone IMAGE:838236 X similar to SW:PRS8 HUMAN P47210 265 PROTEASE REGULATORY SUBUNIT 8; 1012 6152 11306 1.0 0 N43182.1 EST-HUMAN EST51124 WATIVI1 Homo, saplens cDNA done 51124 similar to DNA-DIRECTED RNA POLYMERASE 11 (alignment Ser and Pro vAth BLASTx or p) 1012 6152 11307 1.0 0 N43182.1 EST-HUMAN EST51i24 WATIVII Horno sapiens cDNA clone 51124 similar to DNA-DIRECTED RNA POLYMERASE il (alignment Ser and Pro vAth BLASTx or p) 1013 6153 11308 1.0 0 4759249NT Homo sapiens TRAF family member-assodated NFKB activator (TANK) mRNA 1013 6153 11309 1.0 0 4759249 NT Homo sapiens TRAF family member- assodated NFKB activator (TANK) mRNA 1017 6157 1.5 0 8922933 NT Homo sapiens hypothetical protein FLA 1196 (FLJ1 1196), mRNA 1031 6171 11324 0.8 0 4758569 NT Homo sapiens heat shock 70ki) protein 98 (mortalin-2) (HSPA9B) mRNA 1049 6188 11339 0.6 0 4826672 NT Homo sapiens cadhedn 6, Wcadhedn (fetal kidney) (CDH6) mRNA 1049 6188 11340 0.6 0 4826672 NT Homo sapiens cadhedn 6, K-cadherin (fetal kidney) (CDH6) mRNA 1053 6192 11344 0.9 0 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FLJ20695), mRNA 1053 6192 11345 0.9 0 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FL.120695), mRNA 10M 6193 r-11 13467 13.0 0 AJ245922.1 NT Homo, sapiens mRNA for alpha- tubulin 8 (TUBA8 gene) S CV Page 171 of 196 Table 4

1056 6195 0.9 0 8923087 NT Homo sapiens hypothetical protein FLJ20080 ( U20080), mRNA 1058 6197 11350 1.1 0 5174384 NT Homo sapiens alkylation repair, alkB homolog (MBH), mIRNA mRNA 3 1066 6204 11359 1.2 0 4758117 NT Homo saplens Death associated protein (DAP3) 1080 6218 11369 1.2 0 BE005208.1 EST-HUMAN MRO-BN01 15-200300-003-h08 BNO1 15 Horno saplens cDNA 1103 6241 11393 1.3 0.7706134 NT Horno sapieins poss)um channel, subfamily K, member 9 (KCNK9), mRNA K,7i-n-ember 9 <CNK9), mRNA 1103 6241 11394 1.3 0 7706134 NT Homo sapiens potassium channel, subfamily 1116 6253 11404 -1.1 0.4826947 NT Homosaplens protein kinase, X-linked (PRKX) mIRNA 1116 6263 11405 1.1 0 4826947 NT Homo saplens.proteln kinase, X-linked (PRKX) mRNA S27a mIRNA 1117 6254 11406 1.9 0 4506712 NT Homo sapiens dbosomal protein (RPS27A) 1119 6256 11408 0.9 0 8923290 NT Homo sapiens hypothed 1 protein FLJ20309 (FLJ20309), mIRNA 1121 6258 11410 3.5 0 A13002059.1 NT Homo sapiens DNA for Human PM, complete cds cds 1123 6260 11411 4.1 0 A13002059.1 NT Homo saplens DNA for Hurnan P2XM, complete Homo sapiens similar to rat integral m embrane glycoprotein POM1 21 (POM1 21 L1), mIRNA 1124 6261 11412 0.7 0 7657468 NT Homosapiens similar to rat Integral membrane glycoproteln POM121 (POM121L1),mRNA 1124 6261 11413 0.7 0 7657468 NT 1128 6264 11416 1.0 0 7706500 NT Homo sapiens Npw38-bindin protein NpwBP (LOC51729), mRNA qb22dl 0.xl SoareMregnanLuterus_NbHPU. Homo sapiens cIDNA clone IMAGE:1697011 3 1129 6265 11417 1.0 0 AI 147650.1 EST HUMAN 1131 6267 11419 1.4 0 A13020710.1 NT Homo sapiens mRNA for KWO903 protein, pardal cds Homo sapiens glutamate decarboxylase 1 (brain, MD) (GAD1), transcript variant GAD25, 1152 6287 11440 0.8 0 7305076 NT mRNA Homo sapiens glutamate decarboxylase 1 (brain, MD) (GAD1), transcript variant GAD25-- 1152 6287 11441 0.8 0 7305076 NT mRNA 1154 6289 11443 0.5 0 A13037835.1 NT Homo sapiens mRNA for KIAM 414 protein, partial cds 1161 6296 11452 1.0 0 4557887 NT Horno sapie s keratin 18 (kR-Tf8-)-mRNA 1191 6323 -1.1 0 7657336 NT Homo saplens mutL (E. colt) homolog 3 (MLH3), mRNA 1206 6338 11494 1.1 0 AF264750.1 NT Homo sapiens ALR-like protein mRNA, pardal cds 1206. 6338 11495 1.1 0 AF264750.1 NT Homo saplens ALR-like protein mRNA, partial cds 1207 6339 11496 1.3 0 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial cds 1208 7909 11497 1.0 0 AF264750.1 NT Homo sapi ns AL-R-like protein mRNA, partial cds 1226 6356 11520 396 0 AF109718.1 NT Horno sapiens chromosome 3 subtelomeric region Homo sapiens chondroltin sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 1227 6357 11521 1.3 0 4503098 NT 1245 6374 1.5 0 Y1 8000.1 NT Homo sapiens NF2 gene 1253 6382 11541 16.0 0 4506718 NT Homo saplens dboc ial protein S2 (RPS2) mIRNA 9 (WBSCR9) mRNA, complete Homo sapiens Wilhams-Bcuren syndrome deletion transcript 1260 6389 11550 1.3_ 0 AF08"79.1 NT cds 1266 6395 11554 0.6 0 A13040940.1 NT Homo sapiens mIRNA for KIAA1507 protein, partial ods 1266 6395 11555 0.6 0 AB040940.1 NT Homo sapiens mRNA for K1AA1 507 protein, partial cds mRNA 127 11568 2.2 0 5174748 NT Homo sapi ns Wolfram yndrome MFS) f--t408 Ir- Page 172 of 196 Table 4

1278 6408 11569 2.2 0 5174748 NT Homo saplens Wolfram syndrome (WFS) mRNA 1278 6408 11570 2.2 0 5174748 NT Homo saplons Wolfram syndrome (WFS) mRNA 1279 6409 0.9 0 AF096156.1 NT Homo sapiens protein phosphatase 2A BR gamma subuffit gene, exon 5 1289 7911 11582 1.1 0 7657529 NT Homo sapiens rhabdoid tumor deletion region protein 1 (RTDRI), mRNA 1289 7911 11583 1.1 0 7657529 NT Homo sapiens rhabdoid tumor deletion region protein 1 (RTDR1), mRNA 1295 6424 11589 0.6 0 5803146 NT Homo saelens ring finger protein 9 (RNF9), mRNA 1296 6425 11590 0.8 0 4508004 NT Homo saplens tric finger protein 173 (ZNF173) mIRNA 1298 6427 11591 1.1 0 5803146 NT Homo sap! ns ring finger protein 9 (RNF9), mRNA 1300 6.429 11593 1.4 0 AB01 1149.1 NT Horno sapiens mRNA for KIAA0577 protein, complete ccis 1301 6430 11594 1.1 0 7661965 NT Homo sapiens KIAA0170 gene product (K1AA0170), mRNA 1302 6431 11595 1.6 0 7661965 NT Homo sapiens KIAA01 70 gene product (K1AA01 70), mRNA 1303 6432 11596 1,4 0 8567387 NT Homo sapiens period (Drosophila) homolog 3 (PER3), mRNA 1303.6432 11597 1.4 0 8567387 NT Homo sapiens period (Drosophila) homolog 3 (PER3), mIRNA 1316 1 11610 1.4 0 M14123.1 NT Human endogenous retrovirus HEIRWK1 0 6443 1386 6513 11679 0.9 0 AJ250014.1 NT Horno sapiens mRNA for Familial Cytindromatosis cyid gene 1393 6 11688 3.3 0 AJ277892,1 NT Homo saplens partial TTN gene for Otin 1396 6523 11692 1.0 0 A1208756,1 EST-HUMAN q938b06.xl Soares testis - NIIT Homo sapiens cDNA done IMAGE:1837427 Ysimilar to WP:T27A1.5 CE14213; 1397 6524 11693 3.0 0 6042206 NT RAN, member RAS oncogene familyHomo sapiens RAN. member RAS oncogene family (RAN), mRNA 1407 6534 11705 0,5 0 4505646 NT Homo sapiensproproteln convertase subdiisinlkexin type 2 (PCSK2) mRNA 1407 6534 11706 0.5 0 4505646 NT Homo sapiens proproteln convertase subdifsinlke)dn "c 2 (PCSK2) mRNA 1409 6536 11709 0.9 0 7705565 NT Homo sapiens K1AA1 114 protein (K1AA11 114), mRNA 1409 6536 11710 0.9 0 7705565 NT Homo sapiens K1AA1 114 protein (K1AA1 114). mRNA 1412 6539 11712 1.9 0 A.1238093.1 NT Homo saelens partial AF-4 gene, exons 2 to 7 and Nu repeat elements 1421 6549 11724 1.3 0 AF038280.1 NT Homo saplens. alpha1- 6fucosyltransferase (alphalw6FucT) gene, exon 7 1432 695-9 11735 3.5 0 4507720 NT Homo saplens Utin (TTN) mIRNA 1432 6559 11736 3.5 0 4507720 NT Horno sapiens titin (TTN) mRNA 1437 6564 11740 1,0 0 U35637.1 NT Human nebutin mRNA, partial cds 1437 6564 11141 1.0 0 U35637.1 NT Human nebulin mRNA, partial cds 1445 6572 11746 1.1 0 AL132999.1 NT Novel human gene on chromosome 20 1447 6574 11747 1.0 0 AL137764.1 NT Novel human gene mapping to chornosome 1 1451 6578 11752 1.2 0 D87077.1 NT Human mRNA for KIAA0240 gene, partial ods 1454. 6581 11755 1.8 0 6912457 NT Horno sapiens caldneurin binding protein 1 (K1AA0330), mRNA 1456 6583 11757 0.6 0 7661965 NT Homo sapiens KIAA0170 gene product (K1AA0170), mRNA 1456 6583 11758 0.6 0 7661965 NT Homo sapiens KIAA0170 gene product (K1AA0170), mRNA 1457 6584 1.0 0 Y07829.2 NT Horno sapiens RFB30 gene for RING finger protein 1462 6589 11763 1.3 0 M60676.1 NT Human von Willebrand factor pseudogene corresponding to exons 23 through 34 1462 6589 11764 1.3 0 M60676.1 NT Human von Willebrand factor Oseudogene corresponding to exons 23 through 34 6621 11791 1.3 0 7706434 NT Homo sapiens hHDC for gomolog of Drosophila headcase (LOC51696), mRNA E1495 6636 11805 1.0 0 AA481172.1 EST-HUMAN aa34a03xl NCI CGAP GM Homo sapiens cDNA done IMAGE:815116 5' 15;POk., Page 173 of 196 Table 4

1515 6642 11808 4.3 0 AF023860.1 NT Cercopithecus aethlops cyclophilin A mRNA, complete cds 1515 6642 11809 4.3 0 AF023860.1 NT Ceroopithecus aethlops cyclophilin A mRNA, complete ods 1517 6644 11812 1.0 0 D10884.1 NT Bovine mRNA for neurocalcin 1519 6646 0.7 0 U78027.1 NT Homo sapiens Bruton's tyrosine kinase (M), alpha-D-galactosidase A (GLA), L44-like ribosomal protein (L441---)and FTP3 (FTP3) genes, complete cds 1620 6647 11815 3.9 0 4505404 NT Homo sapiens transmembrane glycoprotein (GPNMB) mRNA 1520 6647 11816 3.9 0 4505404 NT Horno sapiens transmembrane glycoproteln (GPNMB) mRNA 1521 6648 11817 1.1 0 7662405 NT Homo sapiens KiAA0957 protein (K1AA0957), mRNA 1522 6649 3.0 0 7656972 NT Homo sapiens TINIF-inducible protein CG12-1 (CG12-1), mRNA 1527 6654 11821 1.8 0 M98478.1 NT Human transgiutaminase mRNA, complete cds 1530 6657 11823 2.1 0 4507720 NT Homo sapiens fitin (TTN) mRNA 1530 6657 11824 2.1 0 4507720 NT Horno sapiens Efin (TTN) mRNA 1531 7919 10.1 0 4506654 NT Horno sapiens ribosomal protein L5 (RPL5) mRNA 1532 6F58- 11825 4.0 0 M14199.1 NT Human laminin receptor (2H5 epitope) mRNA, Send 1541 6668 11838 2.1 0.4507720 NT Horno saplens fifin (TTN) mRNA 1541 6668 11839 2.1 0 4507720 NT Homo, sapiens fifin (TTN) mRNA 1543 6670 11840 4.7 0 4503098 NT Homo sapiens chondroidn sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 1562 6689 11857 0.6 0 Z83738.1 NT H.sapiens hH2B/e gene 1563 6690 11858 1,4 0 5921460 NT Horno sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA 1563 6690 11859 1.4 0 5921460 NT Homo sapiens butyropoilin. subfamily 2, member All (BTN2A1), mRNA 1566 7920 11862 0.5 0 A13040905.1 NT Homo sapiens mRNA for KIAA1472 protein, partial cds 1570 6696 11863 1.0 0 AF157476.1 NT Homo, saplens DNA polymerase zeta cataUc subunit (REV3) mRNA, Gomplete ods 1572 6698 11866 0.9 0 7662183 NT Homo sapiens KIAA0569 gene product (K1AA0569), mRNA 1572 6698 11867 0.9 0 7662183 NT Homo sapiens KIAA0569 gene product (K1AA0569), mRNA 1574 6700 11868 5.1 0 5729876 NT Homo saplens heat shock 70k13 protein 10 (HSC71) (HSPA110), mRNA 1574 6700 11869 5.1 0 5729876 NT Homo sapiens heat shock 70ki) protein 10 (HSC71) (HSPA10), mRNA 1576 6702 11871 0.9 0 M91 803.1 NT Human sodium channel mRNA 1591 6717 11887 1.7 0 H26973.1 EST-HUMAN yo76c05.sl Soares adult brain N2b41-JB55Y Homo sapiens cDNA clone IMAGE:183848 3' 1645 6771 11942 1.3 0 A1768104.1 EST HUMAN wg81b07.xl Soares_NSF F8_9W_QT_PA.P S1 Homo sapiens cDNA clone 67751 IMAGE:2371477 Ysimilar to TR:Q62788 Q62788 CYWHIS2 ZINC FINGER PROTEIN.

1646 677-2 11943 1.2 0 AF057177.1 NT Horno sapiens T-cell receptor gamma V1 gene region 1650 11947 0.6 0 M29580.1 NT Human zinc-finger protein 7 (ZFP7) mRNA, complete cds 1650 6775 11948 0.6 0 M29580.1 NT Human zino-finger protein 7 (ZFP7) mIRNA, complete cds 1652 6777 11950 1.2 0 4557887 NT Horno, saplens keratin 18 (KRT118) mRNA 1653 6778 11951 0.9 0 7657065 NT Homo sapiens v-ets avian crythroblastosis virus E26 oncogene related (ERG), jnRNA 1657 6781 11955 1.1 0 4557610 NT Homo sapiens gamma-aminobutydc acid (GABA) A recep r, gamma 2 (GA13RG2) mRNA 16W-11 6783 11958 1.1 0 H30132.1 EST HUMAN yo59e08xl Soares breast 3NbHBst Homo sapiens cDNA clone IMAGE:182246 5'sImilar to gb:M64099 GAMMA-GLUTAMYLTRANSPEPTIDASE 5 PRECURSOR (HUMAN); 13 (---I -1 Page 174 of 196 Table 4

1659 6783 11959 1.1 0 H30132.1 EST HUMAN yo59e08xl Soares breast 3NbHBst Homo sapiens cIDNA done IMAGE:182246 5'sImilar to gb:M64099 GAMMA-GLUTAMYLTRANSPEPTIDASE 5 PRECURSOR (HUMAN); 1661 6785 11961 1.2 0 Z80780.1 NT H.sapiens 2131h gene 1661 6785 11962 1.2 0 Z80780.1 NT H.sapiens H2B/h gene 1664 6788 2.8 0 5031748 NT Homo saplens high-mobility group (nonhistone chromosomal) protein 17 (HIVIG17), mRNA 1672 6796 11974 1.6 0 8923841 NT Homo sapiens F0M2 forkhead factor (LOC55810), mRNA 1677 6801 11980 1.0 0 M75980.1 NT Human hepatocyte growth factor gene, exon 15 1677 6801 11981 1.0 0 M75980.1 NT -Humanhepa cyte growth factor gene, exon 15 1680 6804. 11985 0.5 0 4826973 NT Homo sapiens RNA binding motif protein, Y chromosome, family 1, member A1 (R13MY1A1) mRNA 1685.6809 11992 0.9 0 A13026542.1 NT Homo sapiens WAVE2 mRNA for WASP- faMily protein. complete cds 1687 6811 0.9 0 S94400.1 NT TCR zeta [human. GenomicimRNA, 365 nt, se ment 1 of 8) 1745 7925 5.0 0 4506718 NT Homo sapiens dbosomal protein S2 (RPS2) mRNA 1749 6871 12049 0.9 0 4557556 NT Homo sapiens E1Abinding protein p300 (E1R300) mRNA 1749 6871 12050 0.9 0 4557556 NT Homo sapiens E1A binding protein p300 (E1P300) mRNA 1751 6873 12053 1.2 0 U63963.1 NT Human CSIX-1 receptor (FIVIS) gene, complete cds, and (SMF) gene, partial ods 1754 6876 1,1 0 W76571.1 EST HUMAN A66g09xl Soares_fetal-bearLNbHH19W Homo saplens cIDNA done IMAGE:345664 5' 1755 7926 12057 1.4 0 4505332 NT Homo sapiens nuclear autoandgenic sperm protein (histone-binding) (NASP) mRNA 1765 6886 12070 2.6 0 U14967.1 NT Human ribosomal protein L21 mRNA, complete cds 1767 6888 12073 1.7 0 A13002331.1 NT Human mRNA for KJAA0333 gene, partial cds 1768 6889 12074 1.6 0 4502264 NT Homo sapiens activating transcription factor 4 (tax-responfflve enhancer element 1367) (ATF4) 1768 6889 12075 1,6 0 4502264 NT mRNA 1768 6889 12076 1.6 0 4502264 NT Homo sapiens activating transcription factor 4 (tax-responsive enhancer element 1367) (ATF4) mRNA Homo saplens activating transcription factor 4 (tax-responsive enhancer element 1367) (ATIF4) mRNA 1781 6902 12090 1.0 0 4504626 NT Homo sapiens immunoglobin superfamily, member 3 (IGSF3) mRNA, and translated products 1781 6902 12091 1.0 0 4504626 NT Homo sapiens Immunoglobin superfamily, member 3 (IGSF3) mRNA, and translated products 1793 6913 12098 3.7 0 6005855 NT Homo sapiens Retina-derived POU-domain factor-1. (RPM), mRNA 1793 6913 12099 3.7 0 6005855 NT Homo sapiens Retina-derived POU-domain factor-1 (RPM), mRNA 1804 6923 12108 1.2 0 4826783 NT Homo sapiens potassium voitag"ated channel, Shab-related subfamily, member 1 (KCNI31) mRNA 1804 12109 1.2 0 Homo sapiens potassium voltage-gated channel, Shabrelated subfamily, member 1 NBI) mRNA 1805 69231 12110 1.6 n LJ0715 nan refinal degeneration slow (RDS) gene, exon 1 6924 6924 12111 nan retina] degeneration slow (RDS) gene, exon 1 1805 (' 1 Page 175 of 196, Table 4

1809 6928 12116 0.5 0 AW207280.1 EST-HUMAN Ul-H-Bil-afn-f-07-0-Ul.sl NCLWAP-Sub3 Homo sapiens cDNA clone IMAGE:2722333 3' 1809 6928 12117 0.5 0 AW207280.1 EST HUMAN Ul-H-Bil-afn-f-07-0-Ul.sl NCI CGAP Sub3 Homo sapiens cIDNA done IMAGE:2722333 3' 1832 6951 12135 0.8 0 BE277465.1 EST HUMAN 601179164F1 N1H MGC_20 Homo sapiens dDNA done IMAGE:3547239 5' 1832 6951 12136 0.8 0 BE277465.1 EST HUMAN 601179164F1 N1H MGC 20 Homo saplens cDNA clone IMAGE:3547239 5' 1852 6970 12155 1.0 0 BE006292.1 EST HUMAN RC2-BN0126-200300-012-b04 BN0126 Homo sapiens cDNA 1881 6999 12183 1.3 0 4506384 NT Horno sapiens RAD1 (S. pombe) homolog (RAD1) mRNA, and translated products 1881 6999 12184 1.3 0 4506384 NT Homo sapiens RAD1 (S. pombe) homolog (RAD1) mRNA, and translated products 1889 7007 0.6 0 AF157476.1 NT Homo saplens DNA polyTerase zeta catalytic subunit (REV3) mRNA, complete ods 1890 7929 12193 1.5 0 M98478.1 NT Human transgiutaminaso mRNA, complete cds 1890 7929 12194 1.5 0 M98478.1 NT Human transgiutaminase mRNA, complete cds 1895 7012 12201 0.8 0 4507464 NT Homo sapiens transforming growth factor, beta 3 (TGFB3), mRNA 1895 7012 12202 0.8 0 4507464 NT Homo sapiens transforming growth factor, beta 3 (TGFB3), mRNA 1903 7021 0.7 0 M55632.1 NT Human topoisomerase 9 pseudogene 1 1905 7930 12210 0.9 0 5901905 NT Homo saplens butyrophilin, subfamily 3, member A2 ffiTN3A2), mRNA 1913 7030 12217 0.7 0 4809282 NT Homo sapiens histidine ammonia-lyase LHAL) mRNA 1913 7030 12218 0.7 0 4809282 NT Homo saplens hisfidine ammonia-lyase (HAL) mRNA 1924 7041 1.1 0 AL163252.2 NT Homo saplens chromosome 21 segment HS21C052 1926 7043 12232 1.2 0 8400716 NT Homo sapiens nebulin (NEB). mRNA 1926 7043 12233 1.2 0 8400716 NT Homo sapiens nebulin (NES), mRNA 1927 7044 12234 2.9 0. 4826638 NT Homo saplens actinin, alpha 4 (ACTN4) mRNA 1927 7044 12235 2.9 0 4826638 NT Homo sapiens actinin, alpha 4 (ACTN4) mRNA 1937 7054 12247 1.2 0 A13018333.1 NT Homo sapiens mRNA for KJAA0790 protein, partial ods 1937 7054 12248 1.2 0 AB018333.1 NT Homo saplens mRNA for KIAA0790 protein, partial cds 1943 7060 12252 0.7 0 M33782.1 NT Human TFEB protein mRNA, partial cds 1943 7060 12253 0.7 0 M33782.1 NT Human TFEB protein mRNA, partial cds 1945 7062 12254 1.3 0 AW193024.1 EST HUMAN W69b01.xl NCI CGAP Panll Homo saplens cDNA done IMAGE:2679913 3' 1945 7062 12255 1.3 0 AW193024.1 EST-HUMAN A69b01.xl NCI CGAP Pad Homc, sapiens cDNA clone IMAGE:2679913 3, 1946 7063 12256 3.0 0 6912457 NT Homo sapiens calcineudn binding protein 1 (K1AA0330), mRNA 1946 7063 12257 3.0 0 6912457 NT Homo saplens caldneurin binding protein 1 (K1AA0330), mRNA IW8 7065 12259 0.9 0 Z47556.1 NT H.sapiens genes for semenogelin 1 and semenogelin 11 1948 7065 12260 0.9 0 Z47556.11 NT H.sapiens genes for sernenogelin 1 and semenogelin 11 1955 7072 12269 0.8 0 AB040946.1 NT Homo sapjens mRNA for K1AA1 513 protein, pardal cds 2011 7125 12325 1,1 0 4557887 NT Homo sapi ns keratin 18 (KIRT1 8) mRNA 2011 7125 12326 1.1 0 4557887 NT Homo sapjens kerafin 18 (KIRT1 8) mRNA 2013 7127 12327 10 0 4503648 NT Homo sapiens coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia 9) (F9) mRNA 2014 7128 12328 2.0 0 4505606 NT Homo sapiens pregnancy-associated plasma protein A (PAPPA) mRNA 2015 6536. 11709 1 0 7705565 NT Homc, saplens WAA1 114 protein (K1AA1 114), mRNA 201F1-16536 11710 1 0 7705565 NT Homo sapiens K1AA1 114 protein (K1AA1 114, mRNA 1 Page 176 of 196 ' Table 4

2017 7130 12330 0.7 0 AA077589.1 EST HUMAN 7B22E10 Chromosome 7 Fetal Brain cIDNA Library Homo sapiens cIDNA done 7B22E10 2017 7130 12331 0.7 0 AA077589.1 EST-HUMAN 7B22E1 0 Chromosome 7 Fetal Brain cIDNA Library Homo sapiens cIDNA done 7B22E1 0 2019 7132 0.9 0 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM1211-1), mRNA 2021 7134 0.6 0 4585863 NT Horno sapiens phosphodiesterase 6A, GMPspedfic, rod, alpha (PIDE6A), mRNA 2022 7135 12334 0.9 0 AF124250.1 NT Horno saplens SH2-containing protein Nsp2 mRNA, complete cds 2024 7137 0.7 0 A1244247.1 EST HUMAN qv90f08.xl NCLCGAPUt2 Homo saplens cDNA done IMAGE:1988871 Ysimilar to contains Alu repetitive element; 2029 7142 12343 0.9 0 X92841.1 NT H.saplens MICA gene 2031 7144 12345 0.5 0 BE326593.1 EST-HUMAN hr62a08.xl NCII-PGAP-Kidl 1 Homo sapiens cIDNA done IMAGE:3133046 Ysimilar to TR997307 P97307 SW2.; 2031.7144 12346 0.5 0 BE326593.1 EST-HUMAN hr62a08.xl NCLPGAP-Kidl 1 Homo sapiens cIDNA done IMAGE:3133046 3 similar to TR:P97307 P97307 SW2.; 2035 7148 12351 0.9 0 7662007 NT Homo sapiens KIAA0218 gene product (K1AA0218), mIRNA 2035 '7148 12352 0.9 0 7662007 NT Homo saplens KRA0218 gene product (K1AA0218). mIRNA 2040 7153 12358 0.9 0 4757807 NT Homo sapiens ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2) mIRNA 2040 7153 12359 0,9 0 4757807 NT Homo saplens ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2) mRNA 2045 7158 12363 1,3 0 4758489 NT Homo sapiens GTP binding protein 1 (GTPBPI) mRNA 2067 7179 1.0 0 U90552.1 NT Human butyrophilin (BTF5) mRNA, complete cds 7180 1.1 0 AF018963.1 NT Homo saplens X-linked juvenile rednoschisis protein (XLIRS1) gene, exon 6 and complete ods 2070 7182 12386 1.1 0 U54777.2 NT Homo sapiens hMSH6 protein (MSH6) mRNA, complete ods 2071 7183 12387 0.7 0 4503756 NT Horno sapiens flavin containing monooxygenase 2 (FIVI02) mRNA 2074 7186 12389 1,2 0 AW752708.1 EST - HUMAN IL3-CTO219-271099-022- GlOCTO219HomosapienscDNA 2076 7188 12391 0.7 0 A19046.40.1 EST HUMAN QV- 65-020399-092 BT065 Horno sapiens cIDNA 2076 1188 12392 0.7 0 A1904640.1 EST-HUMAN QV-BT065-020399-092 ST065 Homo sapiens cIDNA 2112 7223 1,2 0 7657252 NT Homo sapiens potassium large conductance caldum-activated channel, subfamily M, beta member 3-11ke (KCNMB3L), mRNA 2132 7242 0.6 0 L14787.1 NT Human DNA-binding protein mRNA, Yend 2138 7248 12454 1,0 0 BE274696.1 EST HUMAN 601122338F1 N1H MGC 20 Homo sapiens cIDNA done IMAGE:3346688 5, 2140 7250 12457 1.1 0 7662017 NT Homo sapiens KIAA0244 protein (K1AA0244), mRNA 2141 7251 12458 3.8 0 AB015610.1 NT Chlorocebus aethlops mRNA for dbosomal protein S4X, complete cds 2141 7251 12459 3.8 0 AB015610.1 NT Chlorocebus aethlops mRNA for dbosomal protein S4X, complete cds 2143 7253 12461 0.5 0 7661569 NT Homo sapiens DKFZP434D1 56 protein (DKFZP434D1 56), mRNA 2145 7255 0,9 0 M19828.1 NT Human apolipoprotein B-100 (apoEl) gene, exons 22 through 29 2148 7258 12465 6.2 0 Y118552.1 NT Homo sapiens HLA-B8null gene 2149 7259 12466 4.1 0 4506648 NT Homo sapiens dbosomal protein 1.3 (RPL3) mRNA 2152 7262 1246 0.9 0 U21556.1 NT Human membrane protein-like protein mRNA, pardal cds 2152 7262 12470 0.9 0 U21556.1 NT Human membrane protein-like protein mRNA, partial ods 2156 7936 12475 0.6 0 AF044962.1 NT Homo sapiens polio virus related protein 2 gene, exon 2 Page, 177 of 196 Table 4 ll>c 2159 7268 12478 0,7 0 BE018750.1 EST-HUMAN bb84e02.yl NIH - MGC - 10 Homo sapiens cDNA done IMAGE:3049082 5'similar to TR:Q15170 Q15170 TRANSCRIPTION FACTOR S-II-RELATED PROTEIN; 2160 7269 12479 0.9 0 AA042813.1 EST-HUMAN zk53c07.sl Soares_pregnant- uteru!s_NbHPU Homo sapiens cIDNA done IMAGE:486540 3 similar to gb:X65857_Osl OLFACTORY RECEPTOR-LIKE PROTEIN HG1VIP07E (HUMAN); 2160 7269 12480 0.9 0 AA042813.1 ESTHUMAN zk53c07.sl Soares_pregnant uterus NbHPU Homo saplens cDNA done IMAGE:486540 3' similar to gb:X65857_cdsl OiFACT6RY RECEPTOR-LIKE PROTEIN HG1VIP07E (HUMAN); 2168 7277 12488 0.8 0 AL163204.2 NT Homo sapiens chromosome 21 segment HS21 C004 2168 7277 12489 0.8 0 AL163204.2 NT Homo sapiens chromosome 21 segment HS21 C004 2169 7278 12490 1.0 0 7662401 NT Homo sapiens KIAA0952 protein (K1AA0952), mRNA 2169 7278 12491 1,0 0 7662401 NT Homo sapiens KIAA0952 protein (K1AA0952, mRNA 2174 7283 1.0 0 U36264.1 NT Human beta-prime-adaptin (BAM22) gene, exon 16 2193 7302 12516 2.7 0 4557556 NT Homo saplens E1A binding protein p300 (EP300) mRNA 2199 7308 12520 0.5 0 7662401 NT Homo sapiens KIAA0952 protein (K1AA0952), mRNA 2206 7315 12528 1.1 0 A13023195.1 NT Horno saplens mRNA for KIAA0978 protein, partial cds 2209 7318 12531 0.9 0 7661965 NT Homo sapiens KIAA0170 gene product (K1AA0170), mRNA 2209 7318 12532 0.9 0 7661965 NT Homo saplens KIAA01 70 gene product (K1AA01 70), mRNA 2212 7320 12534 1.4 0 A13037784.1 NT Homo sapiens mRNA for KIAA1363 protein, partial cds 2254 7360 12568 0.9 0 A1076404.1 EST-HUMAN oz09c07xl Soares fetai_iiver_spieen_l NIZI-S-S1 Homo sapiens ciDNA clone IMAGE:1674828 3' 2256 7362 12570 0,8 0 AA429001.1 EST HUMAN zv78al 1.rl Soares-totai-fetusNb2HF8._gw Homo sapiens cDNA clone IMAGE:759740 5, 2256 7362 12571 0.8 0 AA429001.1 EST HUMAN zv78al 1xl Soares-total-fe. tus-Nb2HF8-gw Homo sapiens eDNA done IMAGE:759740 5' 2258 7364 12573 0.8 0 AJ010230.1 NT Horno saplens RET finger protein-like 1 antisense transcript, partial 2263 7369 12579 1.2 0 4826783 NT Homo sapiens potassium voltage-gated channel, Shab-related subfamily. member 1 (KCNB1) mRNA 2264 7370 12580 0.7 0 6325466 NT Homo sapiens flavin containing monooxygenase 3 (FM03), mRNA 2271 7377 12586 1.0 0 A1347926.1 EST HUMAN qp60f09.xl NCI CGA12 Co8 Homo sapiens cIDNA clone IMAGE: 1927433 & 2274 7380 12588 3.7 0 AF044571.1 NT Homo sapiens phosphorylase kinase alpha subunit (PHKA2) gene, exon 32 2275 7381 12589.1.0 0 A1625542.1 EST-HUMAN W57c08.xl NCI CGAP U1:2 Homo sapiens cDNA done IMAGE:2283182 3' 2280 7386 12593 0.6 0 5803178 NT Homo sapiens sperm specific antigen 2 (SSFA2), mRNA 2280 7386 12594 0.6 0 5803178 NT Homo sapiens sperm specific antigen 2 (SSFA2), mRNA 2291 7395 12604 1.5 0 AF058332.1 NT Homo sap! ns titin (TTN) gene, alternative splice products, partial cds 2291 7395 12605 1.5 0 AF058332.1 NT Homo sapiens titin (TTN) gene, alternative splice products, partial cds 2300 7404 12611 1.0 0 5174678 NT Homo sapi ns signal regulatory protein, beta, 1 (SIRP-BETA-11) mRNA 2304 7407 12615 0 AF246302.1 NT Homo sapiens peroxisome proliferative activated receptor delta (PPARD) gene, exons 7 and 7408 2.1 0 450659i NT Homo saplens dbosomal protein L12 (RPL12) mRNA 7409 1 1.0 0 AW867076.1 EST HUMAN IMRI-SNO033-120400-002-a04 SNO033 Homo sapiens cIDNA 12616 Page 178 of 196 Table 4 c 2307 7410 12617 0.7 0 7662017 NT Homo sapiens KIAA0244 protein (KiAA0244), mIRNA 2308 7411 12618 0.5 0 4758497 NT Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1 -dehydrogenase) (H6P0), mIRNA 2308 7411 12619 0.5 0 4758497 NT Horno sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PID), mRNA 2310 7413 12620 2.7 0 4505332 NT Homo sapiens nuclear autoandgenic sperm protein (histone-binding) (NASP) mRNA 2310 7413 12621 2.7 0 4505332 NT Homo sapiens nuclear autoandgenic serm protein (histone-binding) (NASP) mRNA 2310 7413 12622 2.7 0 4505332 NT Homo sapiens nuclear autoandgenic sperm protein (histone-binding) (NASP) mRNA 2327 7430 1.0 0 8922550 NT Homo sapiens hypothetical protein FLJ10618 (FLJ10618), mRNA 2366 7468 1.3 0 AF012872.1 NT Horno sapiens phosphaddylinositot 4-kinase 230 (p14K230) mRNA, complete cds 2369 7471 12673 1.0 0 AW303998.1 EST-HUMAN xvl5f07.xl Soares-NFGBC-SI Horno.sapiens cDNA done IMAGE:2813221 Ysimilar to TR:054924 054924 EX084.; 2382 7484 0.6 0 AF056332.1 NT Horno sapiens AK2B processed pseudogene 2386 7487 12690 2.0 0 6006002 NT Horno sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRiN2A) mRNA 2390 7490 12694 0.9 0 D85606.1 NT Homo sapiens gene for choleWtokinin type-A receptor, complete cds 2390 7490 12695 0.9 0 D85606.1 NT Horno sapiens gene for cholecystokinin type-A receptor, complete cds 2398 7408 12704 0.8 0 AF106275.1 NT Homo sapiens immunogtobutin-like transcript I c variant 4 (ILT1c) gene. exon 6 2410 7509 12717 1,4 0 5729777 NT Homo sapiens collagen, type XII, alpha 1 (COL12A1), mRNA 2414 7513 12720 0.9 0 4507280 NT Horno sapiens serinelthreonine kinase 9 (STK9) mRNA 2414 7513 12721 0.9 0 4507280 NT Homo sapiens sednelthroonine kinase 9 (STK9) mRNA 2419 7518 12725 0.8 0 4557887 NT Homo sapiens keratin 18 (KIRT18) mRNA 2428 7527 12733 1.0 0 AW466922.1 EST HUMAN haO4h04.xl NCL2GAP K1d112 Homo saplens cIDNA clone IMAGE:2872759 3' 2430 7529 12734 1.3 0 AW501010.1 EST-HUMAN Ul-HF-BPOp-ais-c-07-0-Ui.rl N1HAGQ_51 Horno sapienh cIDNA clone IMAGE:3072780 5' 2444 7543 12751 0.8 0 5453965 NT Homo sapiens protein kinase, AMP- activated, alpha 2 catalytic subunit (PIRKAA2) mRNA 2444 7543 12752 0.8 0 5453965 NT Homo sapiens protein kinase. AMP- activated, alpha 2 catalytic subunit (PIRKAA2) mRNA 2457 7556 0.9 0 AW813853.1 EST-HUMAN RC3-ST0197-300300-016-c04 ST0197 Homo sapiens cIDNA 2462 7561 12765 5.8 0 4506744 NT Homo sapiens ribosomal protein S9 (RPS9) mIRNA 2463 7014 12203 1.2 0 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA 2467 7565 12768 0.8 0 Z32684.2 NT Homo sapiens mRNA for membrane transert protein (XK gene) 2469 7567 2.0 0 5453871 NT Homo sapiens platelet-derived growth factor receptor-like (PIDGFIRL) mRNA 2471 7569 12771 0.7 0 AF058953.1 NT Homo saplens ATP-specific succlny]- CoA synthetase beta subunit (SCS) mRNA, partial cds 2472 7570 12772 0.7 0 7657468 NT Homo sapiens similar to rat Integral membrane glycoprotein POM121 (POM1211-1), mRNA 2473 7571 12773 20.0 0 4507720 NT offlo sapiens fifin (TTN) mRNA 2474 7572 12774 1.1 0 8923340 NT Horno sapiens hypothetical protein FLJ20366 (FIL.120366), mRNA 2479 7573 12775 1.2 0 4507524 NT Horno saplens translocation protein 1 (TILOC1) mRNA Page 179 of 196 Table 4

2481 7579 12781 0.6 0 8051635 NT Homo sapiens exportin, tRNA (nuclear export receptor for tRNAs) (XPOT), mRNA 2486 7583 12787 1.6 0 A1804747.1 EST HUMAN tu42cl 1.xl NCLWAP-Pr28 Homo sapiens cDNA done IMAGE:2253716 Ysimilar to contains element MSR1 repetitive element; 2486 7583 12788 1.6 0 A1804747.1 ESTHUMAN tu42c11.xl NCI - CGAP - Pr28 Homo sapiens cDNA done IMAGE:2253716 Ysimilar to contains element MSR1 repetitive element; 24887585 12791 1.0 0 AW888221.1 EST HUMAN WRA5 Human matrix tissue expression library Homo sapiens cIDNA clone Incyte 1996726 similar to WRAS Matrix remodefing associated gene 5 2509 7605 12806 1.4 0 4557336 NT Homo sapiens argininosuccinate synthetase (ASS), mRNA 2509 7665 12807 1.4 0 4557336 NT Homo saplens argininosuccinate synthetase S), mRNA 2510 7606 12808 1.2 0 4506402 NT Homo sa-plens refinoic acid Induced 3 (RA13) mRNA 2510 7606 12809 1.2 0 4506402 NT Homo sapiens refinoic acid Induced 3 (RA13) mRNA 2511 7607 12810 1.0 0 AW589700.1 EST-HUMAN hg19e07.xl NCl-WAP-GC6 Homo saplens cDNA done IMAGE:2946084 Ysimilar to TR:000246 000246 HYPOTHETICAL 9.3 KID PROTEIN; 2514 7610 12812 2,8 0 AW937782.1 EST-HUMAN OV3-DT0045-140200-082-el 1 DT0045 Homo sapiens ciDNA 2540 7636 12836 1.1 0 BE296613.1 EST-HUMAN 601173631 F1 N11-1_1wiGCJ 7 Homo sapiens cDNA done IMAGE.3529159 6 2553 7876 12850 0.9 0 AB037836.1 NT Homo sapiens mRNA for KIAA1415 protein, partial ods 2553 7876 12851 0.9 0 AB037836.1 NT Homo sapiens mRNA for KIAA1415 protein, partial cds 2554 7648 1.4 0 BE084725.1 EST HUMAN MRO-BT0659-280200-003-c06 BT0659 Homo sapiens cDNA 2560 7654 12857 1.0 0 8923955 NT Horno sapiens PC326 protein (PC326), mRNA 2562 7656 1.2 5 4504866 NT Homo sapiens ring finger protein (C31-IC4 type) 8 (RNFI3), mRNA 2569 7662 12864 1.3 0 AB037742.1 NT Horno sapiens mRNA for KIAA1321 protein, partial cds 2570 7663 12865 1.0 0 A1571737.1 EST HUMAN W9b08.xl NCLWAP_Brn25 Homo sapiens cIDNA done IMAGE:2168055 Ysimilar to gb120977 CALCIUM-TRANSPORTING ATPAS6 PLASMA MEMBRANE, BRAIN ISOFORM 2 (HUMAN); 2571 7664 12866 0.8 0 5032150 NT Homo sapiens TATA box binding protein (TBP)-assodated factor, RNA polymerase 11, 1, 28k13 (TAF21) mRNA 2573 7666 12868 2.1 0 AB037859.1 NT Homo sapiens mRNA for KIAA1438 protein, partial ods 2574 7667 12869 1.0 0 AF129756.1 NT Horno sapiens MSH55 gene, partial cds; and CLICI, DDAH, G6b, G6c, G5b, G6d, G6e, G6f.

BAT5, G5b, CSK213, BAT4, G4, Apo M, BAT3, BAT2, AIF-1, 1C7, LST-1, LTB, TW, and LTA genes, complete, cds 2574 7667 12870 1.0 0 AF129756.1 NT Homo sapiens MSH55 gene, partial c.ds; and CLIC1, DDAH, G6b, G6c, G5b, G6d, G6e, G6f, BAT5, G5b, CSK2B, BAT4, G4, Apo M, BAT3, BAT2, AIF-1, 1C7, LST-1, LTB, TW, and LTA genes, complete cds 2577 7670 12871 1.1 0 BE293328.1 EST HUMAN 601143722F1 NIH_MGQ_15 Homo sapiens cIDNA clone IMAGE:3051389 5' 2585 7678 3.8 0 L19185.1 NT Human natural killer cell enhancing factor (NKEFB) mRNA, complete cds 2595 7687 12890 0.9 0 4504686 NT Homo sapiens IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1) mRNA 2601 7949 12897 2.5 0 4507720 NT Horno, sapiens titin C7N) mRNA 2608 7698 1.1 0 U78027.1 NT Homo sapiens Bruton's tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like dbosomal protein (L441---)and FTP3 (FTP3) genes, complete cds 2609 7699 12901 1.9 0 AF173227.1 NT Homo sapiens guanylate cyclase- activating protein 2 (GUCA1 B) gene, exon 1 7703 12902 1.2 0 AB011108.1 Homo saplens rnRNA for KIAA0536 protein. partial cds Page 180 of 196 Table 4 f 7 1 2616 7706 12905 1.0 0 ABOO2299.1 NT Human mRNA for KLAA0301 gene, partial cds 2617 7707 12906 0.5 0 4502442 NT Homo sapiens bullous pemphigold anfigen 1 (230124OkD) (13PAG1) mRNA 2619 7709 12908 1.2 0 U22377.1 NT Human Zn-15 related zinc finger protein (do mRNA, complete cds 2619 7709 12909 1.2 0 U22377.1 NT Human Zn-15 related zinc finger protein (do mRNA, complete cds 2622 7712 12912 1.3 0 AW887015.1 EST-HUMAN RC1-OT0086-220300-01 1-d07 OT0086 Homo saplens cIDNA, 2626 7716 12917 1.0 0 AA92.1 EST-HUMAN ou58e08.sl NCI CGAP Br2 Horno sapiens cDNA done IMAGE:1632038 3' 2627 7717 12918 1.2 0 AF265208.1 NT Homo sapiens SW1-SW complex protein p270 mRNA, partial cds 2628 7718 1.0 0 7662223 NT Homo sapiens KIAA0649 gene product (K1AA0649), mRNA 2656 7745 12945 0.6 0 8922843 NT Homo sapiens hypothetical protein FLJ 11052 (FI-J1 1052), mRNA 2690 7777 3.2 0 AA316723.1 EST-HUMAN EST1 88414 HCC cell line (matastasis to liver In mouse) 11 Homo sapiens cIDNA Send similar to ribosomal protein 129 2691 7778 12975 0.9 0 J03537.1 NT Human ribosomal protein S6 mRNA, complete cds 2698 7784 12984 1.3 0 U36253.1 NT Human beta-prime-adaptin (BAM22) gene, exon 5 2700 7786 12986 1.1 0 7669517 NT Homo sapiens neuregulin 1 (NRGI), transcript variant SM13F, mRNA 2701 7787 12987 3.7 0 AF1 10763.1 NT Homo sapiens skeletal muscle LIM- projeln 1 (FHLI) gene, complete cds 2708 7793 12994 10.4 0 Y00052.1 NT Human mRNA for T-cell cyclophilin 2709 7794 12995 3.2 0 T07303.1 ES HUMAN EST05192 Fetal brain, Stratagene (cat#936206) Homo sapiens cIDNA clone HFBEG84 similar to Ring canal protein 2712 7953 12999 13.5 3 4506696 NT Homo saplens ribosomal protein S20 (RPS20) mRNA 2713 7797 1.3 0 A1065139.1 EST HUMAN HA0977 Human fetal liver cIDNA library Homo sapiens cDNA 2715 7799 13002 0.8 0 5174486 NT Horno sapiens spermatogenesis associated PD1 (K1AA0757) mRNA 2715 77U9 13003 0,8 0 5174486 NT Homo saplens spermatogenesis associated PD1 (K1AA0757) mRNA 2716 7800 13004 0.9 0 8923441 NT Homo saXns hypothetical protein FLJ20477 (FLJ20477), mRNA 2716 7800 13005 0.9 0 8923M1 NT Homo sapiens hypothetical protein FLJ20477 (FL.120477), mRNA 2717 7801 13006 0.8 0 7661839 NT Homo sapiens HT002 protein (HT002), mRNA 2718 7802 5.7 0 M1 1147.1 NT Human ferddn L chain mRNA, complete cds 2719 7803 13007 0.6 0 U21556.1 NT Human membrane protein-like protein mRNA, partial ods 2719 7803 13008 0,6 0 U21556.1 NT Human membrane protein-like protein mRNA, partial cds 2723 7807 13011 1,2 0 4757963 NT Homo sapiens cerebellar degeneration- related protein (34kD) (CDRI) MRNA 2723 7807 13012 1.2 0 4757963 NT Horno sapiens cerebellar degeneradon- related protein (34kID) (CDR1) mRNA 2727 7811 13017 0.8 0 4504918 NT Homo sapiens keratin 8 (KRT8) mRNA 2730 7814 13020 1.0 0 BE176836.1 EST-HUMAN RC4-HT0587-170300-012-dl 1 HT0587 Horno sapiens cDNA 2741 7825 1.3 0 AL1 63201.2 NT Horno sapiens chromosome 21 segment HS21 C001 2742 7826 13030 1.3 0 D63879.1 NT Human mRNA for KIAA0156 gene, complete cds 2748 7832 1.1 0 4503098 NT Horno sapiens chondroltin sulfate proteoglycan 4 (melanoma-assodated) (CSPG4), mRNA 2758 7842 13045 0.6 0 7427522 NT Homo sapiens protein tyrosine phosphatase, receptor type, T (PTPRT), mRNA 2761 7845 13047 3.5 0 AA490647.1 EST HUMAN aa01g04xl Soares_Nhi-IMPu-S1 Homo sapiens cIDNA done IMAGE:812022 5'similar to SW2N22-HUMAN P17026 ZINC FINGER PROTEIN 22;contains element LTR6 repetitive element; Page 181 of 196 Table 4 cj 2761 7845 13048 3.5 0 AA490647.1 EST-HUMAN aa01g04xl Soares_Nhi-IMPuSI Homo sapiens cDNA done IMAGE:812022 C similar to SW2N22 - HUMAN P17026 ZINC FINGER PROTEIN 22xontains element LTR6 repetitive element; 2763 7847 4.0 0 6912633 NT Horno sapiens dbosomal protein L13a (RPI-13A), mRNA 2766 7850 13053 0.7 0 AF036943.1 NT Homo sapiens myelin transcription factor 1 -like (MYT14) mRNA, complete cds 2767 7851 13054 1.1 0 4504918 NT Homo sapi ns keratin 8 (KRT8) mRNA 2769 7853 13055 1.1 0 AW505486.1 EST-HUMAN Ul-HF-BNO-amb-d-02-0-Ul.rl NIH- MGC-50 Homo sapiens cDNA clone IMAGE:3081626 6 2769 7853 13056 1,1 0 AW505486.1 EST HUMAN Ul-HF-BNO-amb-d-02-0-Ul.rl N1HAGQ_50 Homo saplens cDNA done IMAGE:3081626 5' 2770 7854 13057 3.7 0 4506648 NT Homo sapiens dbosornal protein 1.3 (RPL3) mRNA 2770 7854 13058 3,7 0 4506648 NT Homo saffiens ribosomal protein L3 (RPL3) mRNA 2775 5373 10498 1.6 0 S76830.1 NT glycoprotein D=Duffy group antigen (human, blood, Genomic DNA, 3068 nt) 2778 7860 0.7 0 A13033281.1 NT Homo saplens BTRCP2 mRNA for F-box and WD- repeats protein isoform C, complete cds 2784 5871 11011 1.4 0 AF264750.1 NT Homo sapiens ALR-like protein mRNA, partial cds 2784 5871 11012 1,4 0 AF264750.1 NT Homosapiens ALR-like protein mRNA, partial cds 2789 6163 11316 1.2 0 4503202 NT Homo sapiens cytochrome P450, subfamily 1 (dioxin-inducible), polypeptide 1 (glaucoma 3, primary Infantile) (CYPI1131) mRNA 2789 6163 11317 1.2 0 4503202 NT Homo sapiens cytochrome P450, subfamily 1 (dioxin-inducible), polypepfide 1 (glaucoma 3, primary Infantile) (CYPIB1) mRNA 2805 7957 13066 2.4 0 X85980.1 NT H.sapiens serine hydroxymethyitransferase pseudogene 2806 7958 1.3 0 AF068624.1 NT Homo sapiens 5-aminolevulinate synthase 2 (ALAS2) gene, complete ods 2808 7960 1A 0 A13040960.1 NT Homo sapiens mRNA for KIAA1527 protein, partial cds 2814 7965 1.1 0 AJ238852.1 NT Homo sapiens partial rp13 gene for dbosomal protein L3, U82 snoRNA, U83a snoRNA and U83b snoRNA genes 2815 7966 13070 2.2 0 AL163201.2 NT Homo sapiens chromosome 21 segment HS21 C001 2819 7970 13073 5.9 0 M80902.1 NT Human AHNAK nudeoprotein mRNA, Vend 2822 7973 13075 1.6 0 BE154504.1 EST HUMAN PMO-HT0343-281299-003-e02 HT0343 Homo sapiens cDNA - 2822 7973 13076 1.6 0 BE154504.1 EST-HUMAN PM0-HT0343-281299-003-e02 HT0343 Homo, sapiens eDNA 2824 7975 1.4 0 X73428.1 NT H.sapiens W3 gene for HLH tyRe transcription factor 2826 797-7 2.8 0 AL163268.2 NT Homo sapiens chromosome 21 segment HS21C068 2828 7979 13079 2.6 0 M98478.1 NT Human transglutominase mRNA, complete cds 2833 7984 13083 43.5 0 D50657.1 NT Homo sapiens gammma-cytoplasmic adfin (ACTGP3) pseudogene 2833 7984 13084 43.5 0 D50657.1 NT Homo sapiens gammma-oplasmic actin (ACTGP3) pseudogene 2837 7988 13087 1.3 0 AL096857,1 NT Novel human mRNA from chromosome 1, which has similarities to BAT2 genes 2838 7989 4.6 0 Y1 0658.1 NT H.sapiens mRNA for nuclear DNA helicase 11 2839 7990 1.0 0 AF152303.11 NT Homo sapiens prolocadherin alpha Cl (PCDH- alpha-Cl) mRNA, complete ods 2840 7991 13088 25.1 0 4503470 NT Homo sapiens eukaryofic translation elongation factor 1 alpha 1 (EEF1A1) mRNA 2840 7991 13089 25.1 0 4503470 NT Homo saple s eukaryofic translation elongation factor 1 alpha 1 (EEF1A1) mRNA 5002 13100 27--- 0 4507280 NT Homo sapiens sednelthreonine kinase 9 (STK9) mRNA 1 Page 182 of 196 Table 4 klIA c-, 1 2854 8005 13104 1.4 0 AL047599.1 EST HUMAN DKFZp586G0621-M 586 (synonym: hutel) Homo sapiens cDNA done DKRp586G06211 2855 8006 13105 1.3 0 7661883 NT Homo sapi ns KIAA0054 gene product; Helicase (K1AA0054), mRNA 2855 8006 13106 1,3 0 7661883 NT Homo sapiens KIAA0054 gene product. Helicase (K1AA0054), mRNA 2856 8007 3.4 0 4503098 NT Homo sapiens chondroltin suffate proteoglycan 4 (melanoma-assodated) (CSPG4), mRNA 2858 8009 13108 5,0 0 BE0811896.1 EST HUMAN QV2-BT0636-130400-138-h03 BT0636 Homo sapiens cDNA 2858 8009 13109 5.0 0 BE081896.1 EST HUMAN QV2-BT0636-130400-138-h03 BT0636 Homo sapiens cDNA 2866 8017 13120 1.6 0 AL1 63206.2 NT Homo sapiens chromosome 21 segment HS21C006 2866 8017 13121 1.6 0 AL1 63206.2 NT Homo saplens chromosome 21 segment HS21C006 2867 8018 13122 1.11, 0 AA215579.1 EST-HUMAN zr96bl 1M NCI CGAF_GCB1 Homo sapiens cIDNA done IMAGE:683517 Ysimilar to contains Alu reetiuve element; 2874 8025 3,1 0 Y19210.1 NT Horno sapiens Iril-1b5 gene for hair keratin, exons 1 to 9 2877 8028 13131 1.2 0 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 2878 8029 13132 18,7 0 4503470 NT Homo sapiens eukaryobc translation elon adon factor 1 alpha 1 (EEF1A1) mIRNA 2879 8030 13133 1.3 0 A1561002.1 EST-HUMAN tn18d07.xl NCI - CGAP_Bm25 Homo sapiens cDNA done IMAGE:2167981 X similar to TR:016247 016247 F44E7.2 PROTEIN.; 2879 8030 13134 1.3 0 A1561002.1 EST-HUMAN 018d07.xl NCI_CGAP_Bm25 Homo sapiens cDNA done IMAGE:2167981 Ysimilar to TR:016247 016247 F44E7.2 PROTEIN 2881 8032 13136 1.9 0 P52740 SWISSPROT ZINC FINGER PROTEIN 132 8033 13137 1.5 0 AF152338.1 NT Homo sapiens protocadherin gamma C4 ( CDHgamma-C4) mRNA, complete cds 2897 8048 13149 1,3 0 A8033093.1 NT Homo sapiens mIRNA for KIAA1267 protein; partial cds 2897 8048 13150 1.3 0 AB033093.1 NT Homo saplens mIRNA for KIAM 267 protein, partial ods 2898 8049 13151 5.0 0 A8040941.1 NT Homo saplens mRNA for KIAM 508 protein, partial ods 2898 8049 13152 5.0 0 AB040941.1 NT Horno sapiens mRNA for KIAA1508 protein, partial ods 2901 8052 13155 2.7 0 7661903 NT Horno sapiens KIAA0100 ge a product (K1AA0100), mIRNA 2901 8052 13156 2.7 0 7661903 NT Homo saplens K1AA0100 gene product (K1AA0100), mRNA 2902 8053 13157 3.2 0 5174574 NT Homo saplens myeloichlymphold or mixed- lineage leukernia (trithorax (Drosophila) homolog); 2902 8053 13158 3.2 0 5174574 NT translocated to, 4 (MI-LT4) mIRNA Homo sapiens myeloidAymphold or mixed-lineage leukernia (trithorax (Drosophila) homolog); translocated to, 4 (MI-LT4) mRNA 2915 8066 13174 2.0 0 4505084 NT Horno sapiens melanoma antigen, family B, 4 (MAGEB4). mIRNA 2915 8066 13175 2.0 0 4505084 NT Homo sapiens melanoma antigen, family 4 (MAGE84), mRNA 2917 8068 13177 0.9 0 4885214 NT Homo sapiens v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4 (ERBB4) mRNA 2917 8068 13178 0.9 0 4885214 NT Homo saplens v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4 (ERBB4,) mIRNA 2924 8075 131 W 1.6 0 4758827 NT Homo sapiens neurexin lit (NRXN3) mRNA 2927 0 13106 1.3 0 X15309.1 NT aplens NF-H gene, exon 4 8 8 18 8078 1 2927 0 13187 1,3 0 X153C iapiens NF-H gene, exon 4 8078 18 1 2929 8080 1 7n rno sapiens immunoglobulin-like transcript lc variant 4 (1LT1 c) gene, exon 6 8080 13189 Page 183 of 196 Table 4

C r 2943 8094 1.1 0 A1149880.1 EST - HUMAN qf43f09.xl Soares testis NHT Homo sapiens cDNA done IMAGE:1752809 3' 2953 8104 13211 1.0 0 4506118 NT Homo - sapiens prospero-related homeobox 1 (PROX1) mRNA 2954 8105 13212 2,2 0 A130048B4.1 NT Homo sapiens mRNA for PW-alpha; partial cds 2964 8115 13221 1.3 0 7662273 NT Homo saplens KIAA0737 gene product (K1AA0737), mRNA 2965 8116 13222 1.6 0 5729755 NT Homo saplens calcium channel. voltagedependent, gamma subunit 3 (CACNG3), mRNA 2965 8116 13223 1.6 0 5729755 NT Homo sapiens calcium channel, voltage- dependent, gamma subunit 3 (CACNG3), mRNA 2990 8142 0.9 0 AL163246.2 NT Homo sapiens chromosome 21 segment HS21C046 2992 8144 13246 1.0 0 M74099.1 NT Human displacement protein (CCAAT) mRNA 8153 13253 0.8 0 4506882 NT Homo sapiens semenogelin 1 (SEMG1) mRNA 3009 8161 13262 7.9 0 5579469 NT Homo sapiens heat shock 70ki) protein 1 (HSPA1A), mIRNA 3009 8161 13263 7.9 0 5579469 NT Homo sapiens heat shock 7OkD protein 1 (HSPAIA), mRNA 3011 8163 5.1 0 AL359403.1 NT Isoform 2 of a novel human mIRNA Corn chromosome 22 3014 8166 13267 2.0 0 AF017433.1 NT Homo sapiens putative transcription factor CR53 (CR53) mRNA, partial cds 3017 8169 1.7 0 AF196779.1 NT Homo sapiens transcription factor IGHM enhancer 3, JM1 1 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, complete ods;,and L-type calcium channel a> 3019 8171 13271 1.0 0 4504664 NT Homo sapiens intedeukin 2 receptor, beta (IL2RB) mRNA 3038 8190 13292 2.8 0 X03529.1 NT Human germline gene 16.1 for lg lambda L-chain C region (19L-C16.1) 3043 8194 1.9 0 AF199355.1 NT Homo saplens F-box protein FB1-5 (F8L5) mIRNA, complete cds 3047 8198 13299 1.9 0 AF064589.1 NT Homo sapiens melanoma-assodated antigen (MAGE-CI) gene, complete cds 3068 8219 13318 2.9 0 AF265208.1 NT Horno, sapiens SW1-SW complex protein p270 mIRNA, partial cds 3069 8220 13319 4.0 0 AF149773.1 NT Homo sapiens NODI protein (NOD1) gene, exons 1, 2, and 3 3074 8225 13323 4.2 0 7662139 NT Homo saplens KIAA0469 gene product (K1AA0469), mRNA 3075 8226 13324 1.6 0 AF042075.1 NT Homo sapiens olfactory receptor-like protein (OLFR 4213) gene, OLFR 42B-91 10 allele, partial ods 3102 8252 13356 3.5 0 4826783 NT Homo sapiens potassium voitag"ated channel, Shab-related subfamily, member 1 (KCNB1) rpIRNA 3111 8260 13364 19,6 0 L20941.1 NT Human ferrifin heavy chain mIRNA, complete cds 3115 8264 13368 1.8 0 AB01 1121.1 NT Homo saple s mRNA for KIAA0549 protein, partial cds 3115 8264 13369 1.8 0 AB01 1121.1 NT Homo sapiens mIRNA for KIAA0549 protein, partial cds 3123 8272 13377 9.4 0 TW70.1 EST HUMAN ye32f03.sl Stratagene lung (937210) Homo sapiens cIDNA done IMAGE:119453 3'similar to SRS29539 S29539 BASIC PROTEIN, 23K -;, 3140 8289 13395 1.0 0 A1968086.1 EST HUMAN wul2h10.xl NCI CGAP GC6 Homo saplens cDNA done IMAGE:2516803 3' 3145 8294 13402 4,0 0 X98922.1 NT H.sapiens mRNA for gamma- glutamAtransferase 3145 8294 13403 4.0 0 X98922.1 NT H.sapiens mRNA for gamma- glutamyltransferase 3156 8305 13415 1.5 0 4758827 NT Homo sapiens neureyJn Ill (NIRXN3) mRNA 3156 8305 13416 1.5 0 4758827 NT Homo sapiens noureyJn lit (NRXN3) mIRNA .8312 13424 7.7 0 4504658 NT mo sapiens intedeukin 1 receptor, type 1 (11- 11R1) mRNA Page 184 of 196 Table 4

3164 8313 13425 3.3 0 4507720 NT Homo sapiens fitin (TTN) mRNA 3164 8313 13426 3.3 0 4507720 NT Homo siplens titin (TTN) mRNA 3180 8329 13442 2.4 0 M28699.11 NT Homo sapiens nudeolar phosphoprotein B23 (NPM1) mRNA, complete cds 3183 8332 13444 2.2 0 4502098 NT Homo sapiens solute carrier family 25 (mitochondrial carrier; adenine nudeofide translocator), member 5 (SLC25A5), nuclear gene encoding mitochonddal protein, mRNA 3188 8337 13450 0.8 0 4758055 NT Homo sapiens CREB binding protein (Rubinstein-Taypi syndrome) (CREBBIP) mRNA 3188 8337 13451 0.8 0 4758055 NT Homo sapiens CRES binding protein (Rubinstein-Taybi syndrome) (CREBBP) mIRNA 3190 8339 13462 26.3 0 AA774783.1 EST-HUMAN ae87bl 11M Stratagene schizo brain S1 1 Homo sapiens c(DNA done IMAGE:971133 3, 3210 8358 13467 1.6 0 4557590 NT Homo sapiens fibrillin 1 (Marfan syndrome) (FBN1) mRNA 3215 8363 13474 3.4 0 4507720 NT Homo sapiens fitin (TTN) mRNA 3224 8372 4.4 0 M65189.11 NT Human connexin 43 processed pseudogene 3225 - 13481 1,2 0 AF019413.11 NT Homo sapiens HLA class fit region containing tenascin X (tenascin-X) gene, partial cds; 8373 cytochrome P450 21-hydroxylase (CYP21B), complement component C4 (C4131) G1 1.

helicase (SK12W), RD, complement factor B (B0, and complement component C2 (C2) genes,> 3227 8375 13483 4.2 0 AF055084.11 NT Homo sapiens very large G-protein coupled receptor-1 (VI-GR11) mRNA, complete ods 3237 10294 13491 3.5 0 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3237 10294 13492 3.5 0 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP.1), mRNA 3252 8399 13505 2.0 0 AF265208.1 NT Homo saplens SWI-SNF complex protein p270 mRNA, partial cds 3253 8400 13506 0.9 0 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FIL.120695), mRNA 3282 8427 13535 4.9 0 A1589294.1 EST HUMAN tr58f08.x2 NCI_PGAP_Panl Homo sapiens cIDNA done IMAGE:2222535 Ysimilar to SW:R1-1111RATP251121 60S RIBOSOMAL PROTEIN L11.;contains AW repetitive element; 3289 8435 13543 3.0 0 An28893.1 NT Homo sapiens telornerase reverse transcdptase (TERT) gene, exons 1-6 3289 8435 13544 3.0 0 AF128893.1 NT Homo saplens telomerase reverse transcriptase (TERT) gene, exons 1-6 3290 8436 13545 1.0 0 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 3290 8436 13546 1.0 0 7657213 NT Homo sapiens hormonally upre ulated neu tumor-assodated kinase (HUNK), mRNA 3292 8438 13547 1.0 0 4502582 NT Homo spiens caspase 8, apoptosis-related cystelne protease (CASP8) mRNA 3292! 8438 13548 1,0 0 4502582 NT Homo sapiens caspase 8, apoptosisrelated cysteine protease (CASP8) mRNA 3296 8442 13551 11.1 0 AF111163.1 NT Homo sapiens pyrin (MEFV) gene, complete cds 3298 8444 13553 1.0 0 AB040940.1 NT Homo sapiens mRNA for KIAA1507 protein, partial ods 3357 8500 13608 1.7 0 7363436 NT Homo sapiens olfactory receptor, family 10, subfamily C, member 1 (OR1 0C1), mRNA 3357 8500 13609 1.7 0 7363436 NT Homo sapiens olfactory receptor, family 10, subfamily C, member 1 (OR10C1), MRNA 3360 8503 13611 1.4 0 7706239 NT Homo sapiens neuroblastoma-amplified protein (LOC51594), mRNA 3351 d5U4 1JU12 llu 0 AF211189.1 NT Homo sapiens T-type calcium channel alphal subunit Alphal ka Isoform (CACNA1 1) mRNA, complete cds 3377 8520 13626 1.4 0 7662401 NT Homo sapiens KIAA0952 protein (K1AA0952), mRNA 3377 8520 13627 1.4 0 7662401 NT Homo sapiens KIAA0952 protein.(KIAA0952), mRNA 3378 8521 136281 1.0 0 4502398 NT Homo sapiens beaded filament structural protein 1, filensin (BIFSP1) mRNA Page 185 of 196 Table 4 __9 3381 8524 13630 1.7 0 5803067 NT Homo sapiens leukocyte immunoglobulin- like receptor, subfamily A (with TM domain), member 2 (LILRA2), mRNA 3390 7787 12987 6.0 0 AF110763.1 NT Homo sapiens skeletal muscle LIM- protein 1 (FHL1) gene, com&ete cds 3395 8537 13643 2.1 0 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA 3398 8540 13647 1.5 0 AJ277276.1 NT Homo saplens mRNA for mpa-2 (rapa gene) 3398 8540 13648 1.5 0 A.1277276.1 NT Horno, sapiens mRNA for rapa-2 (rapa 9e2Je 3400 8542 13650 5.5 0 K02380.1 NT Bacteriophage P1 replication region Including repA, parA, and parB genes and incA, incB, and incC Incompatibility determinants 3402 8544 13652 1.2 0 7427522 NT Horno sapiens protein tyrosine phosphatase, receptor type, T (PTPRT), mRNA 3409 8551 13658 3.7 0 A1935159.1 EST HUMAN wp14d10.xl NCI_CGAP-Lulg Homo saplens cDNA done IMAGE:2464819 Ysimilar to TR:073634 073634 NEURAL CELL ADHESION MOLECULE.; 3409 8551 13659 3.7 0 A1935159.1 EST HUMAN wp114d10.xl NCI - CGAP - Lulg Horno sapiens cDNA done IMAGE:2464819 Ysimilar to TR:073634 073634 NEURAL CELL ADHESION MOLECULE_.

3413 8555 13664 2,7 0 AJ278120.1 NT Homo sapiens mRNA for putative ankyrin-repeat containing protein (ORF1) 3420 8562 13673 2.9 0 6552332 NT Horno, sapiens v-fos FBJ mudne osteosa=ma viral oncogene homolog (FOS), mRNA 3420 8562 13674 2.9 0 6552332 NT Homo sapiens v-fos FB.) murine osteosarcoma Viral oncogene homolog (FOS), mRNA 3426 8568 13680 1.1 0 M14123.1 NT Human endogenous retrovirus HEW-K10 3431 8573 13685 6.2 0 U43293.1 NT Human MDS1A (AML1IMDS1 fusion) mRNA, partial ods 3440 8582 13695 2.1 0 AF045452.1 NT Homo sapiens cell-line KG1 transcriptional regulatory protein p54 mRNA, complete cds 3440 8582 13696 2.1 0 AF045452.1 NT Horno sapiens cell-line KG1 transcriptional regulatory protein p54 mRNA, complete cds 3448 8590 13704 1.2 0 AF231922.1 NT Homo sapiens chromosome 21 unknown mRNA 3455 8596 13707 0.9 0 AA626677.1 EST HUMAN ab51f12.rl Stratagene lung carcinoma 937218 Homo sapiens eDNA done IMAGE:844367 51 3455 8596 13708 0.9 0 AA626677.1 EST HUMAN ab51f12xl Stratagene lung carcinoma 937218 Homo sapiens cDNA done IMAGE:844367 51 3455 8596 13709 0.9 0 AA626677.1 EST HUMAN ab51f12.rl Stratagene lung carcinoma 937218 Homo sapiens cDNA clone IMAGE:844367 51 3458 8599 13712 1.1 0 4508028 NT Homo sapiens zinc finger protein 45 (a Kruppel-assodated box (KRAB) domain polypeptide) (ZNF45) mRNA 3461 8602 13714 2.2 0 BE304791.1 EST-HUMAN 601143853F1 N1H MGC 15 Homo sapiens cDNA done IMAGE:3051373 5' 3461 8602 13715 2.2 0 BE304791.1 EST-HUMAN 601143853F1 NIH WCJ 5 Homo saplens cDNA done IMAGE:3051373 5, 3463 8604 13717 1.4 0 4826795 NT Homo sapiens potassium voltage-gated channel, Isk-related family. member 2 (KCNE2) mRNA 3470 8611 13723 0.9 0 A1384007.1 EST-HUMAN te35g12.xl Soares - NhHMPu - S1 Horno sapiens ONA. done IMAGE:2088742 Ysimilar to TR:000498 000498 MYASTHEMA, GRAVIS AUTOANTIGEN GRAVIN; 3473 8614 13726 1.0 0 M10976.1 NT Human endogenous retroviral DNA (M), complete retrovimi segment 3495 8637 13749 0.8 0 4506884 NT Homo sapiens semenogelin 11 (SEMG2) mRNA 3497 8639 1.3 0 AF078868.1 NT Homo sapiens homologous yeast-44.2 protein mRNA, complete cds 3505 8647 13757 1.4 0 AL1 33204.1 NT Novel human gene mapping to chomosome X 3507 BW 13758 1.0 0 AB040909.1 NT Homo sapiens mRNA for KIAA1476 protein, partial cds Page 186 of 196 1 Table 4 r_ F e- 3527 8668 1.0 0 A1081907.1 ESTHUMAN ox77c11.xl Soares_Nhi-IMPu-SI1 HomosaplenscIDNA done IMAGE:1662356 Ysimilar to WRT19134.4 CE113742; 3529 8670 13774 1.3 0 6325463 NT Homo sapiens butyrophilin, subfamily 3, member A3 ffiTN3A3), mRNA 3533 8674 4.5 0 AW852217.1 EST-HUMAN QVO-CTO225-230300-169-e01 CTO225 Homo sapiens cDNA 3540 8681 1.0 0 AF1 18846.1 NT Homo sapiens gamma-glutamAcystelne synthetase (GLC) gene, partial cds 3564 8703 1.1 0 4826967 NT Homo saplens retinoblastoma-binding protein 2 (RBBP2) mRNA 3566 8705 13801 1.0 0 AW664693.1 EST-HUMAN h184g01.xl Soares NFI.J GBC S1 Ho o sapiens cIDNA done IMAGE:2979024 3' 3566 8705 13802 1.0 0 AW664693.1 EST HUMAN W84g01.xl Soares NFI-j_GI3C S1 Homo sapiens cIDNA done IMAGE:2979024 3' 3569 8708 13806 1.1 0 4826763 NT Homo sapiens heparan sulfate (glucosamine) 3-0-suifotransferase 1 (HS3ST1) mRNA 3571 8710 13809 1.0 0 7662319 NT Horno sapiens KIAA0806 gene product (K1AA0806), mIRNA 3578 8717 13814 0.8 0 4557752 NT Homo sapiens midline 1 (Opitz/BBB syndrome) (MID1) mIRNA 3578 8111 13815 0.8 0 4557752 NT Homo sapiens midline 1 (Opit7JBBB syndrome) (MID1) mRNA 3596 8735 13829 1.5 0 D87327.1 NT Homo sapiens mIRNA for G protein- coupled Inward rectifier potassium channel, complete cds 3600 8739 28.7 0 7669491 NT Homo saplens glyceraidehyd -3-phosphate dehydrogenase (GAPD), mRNA 3616 8755 13848 4.3 0 A13026542.1 NT Homo sapiens WAVE2 mRNA for WASP- family protein, complete cds 3619 8758 13850 3.3 0 AF124250.1 NT Homo sapiens SH2-containing protein Nsp2 mRNA, complete cds 3619 8758 13851 3.3 0 AF124250.1 NT Homo sapiens SH2-containing protein Nsp2 mRNA, complete cds 3623 8762 13855 1.5 0 AA852743.1 EST-HUMAN NI-ITI3Cael 5909fl Normal Human Trabecular Bone Cells Homo sapiens cIDNA done NI-ITI3Cae15g09 3623 8762 13856 1.5 0 AA852743.1 EST - HUMAN NHTI3Caell 5g09fl Normal Human Trabecular Bone Cells Homo sapiens cIDNA done NI-ITBCae15g09 3626 8765 13858 2.2 0 AL163204.2 NT Homo sapiens chromosome 21 segment HS21C004 3626 8765 13859 2.2 0 AL163204.2 NT Homo sapiens chromosom721 segment HS21 C004 3630 8769 13863 1.8 0 5729928 NT Homo saplens matrix metalloproteinase 24 (membrane-inserted) (MIVIP24), mRNA 3632 8771 13865 1.3 0 A13018339.1 NT Homo sapiens mRNA for KIAA0796 protein, partial cds, 3645 8784 13876 3.5 0 AW298134.1 EST-HUMAN Ul-H-BWO-ajs-e-12-0-Ul.sl NCI- CGAP_Sub6 Horno sapi ens cIDNA clone IMAGE:2733022 X 3645 8784 13877 3.5 0 AW298134.1 EST HUMAN Ul-H-BWO-ajs-c-12-0-Ul.sl NCI_PGAP-SO6 Homo sapiens cIDNA clone IMAGE:2733022 3' 3668 8807 13901 1.0 0 ABOO4630.1 NT Human gene for Type XIX collagen al chain, exon 6 3669 8808 13902 1.0 0 AA463659.1 EST---HUM N aa06901xl Soares_Nhi-IMPu_S1 Homo saplens cIDNA done IMAGE:812496 5'similar to SW:KR134_SHEEP P02445 KERATIN, HIGH-SULFUR MATRIX PROTEIN, 111134. [1]; 3677 8816 13909 3.4 0 7657468 NT Homo sapiens similar to rat Integral membrane glycoprotein POM121 (POM1 21 Ll), mRNA 3687 8825 13919 0.9 0 A13037835.1 NT Homo sapiens mRNA for KIAA1414 protein, partial ods 3699 8838 1 13929 3.9 0 7662183 NT Horno sapiens KIAA0569 gene product (K1AA0569), mRNA 3701 8840 13931 7.9 0 4506718 NT Horno sapiens dbosomal protein S2 (RP 2) mRNA 3705 8843 139331 1.0 0 7657065 NT Homo sapiens v-ets avian erythroblastosis virus E26 oncogene related (ERG), mIRNA 3705 8843 139341 1.0 0 7657065 NT Homo saplens v-ets avian erythroblastosis virus E26 oncogene related (ERG), mIRNA 37M 1 8884 13973 1.1 0;AF145712.1 NT Homo sapiens soluble neuropilin-1 mRNA, complete cds Page 187 of 196 Table 4

3747 8885 1.0 0 AF1 95658.1 NT Homo saplens DNA mismatch repair protein (MILH3) gene, complete cds 3748 8886 13974 2.3 0 AF1 79733.1 NT Pan troglodytes olfactory receptor (PTIR208) gene, partial cds 3751 8889 13978 1.7 0 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM 121 (POM 121 Ll), mRNA 3751 8889 13979 1.7 0 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM1 21 (POM 121 L1), mRNA 3752 8890 13980 1,5 0 AF020091.1 NT Homo sapiens smooth muscle myosin heavy chain SMI mRNA, alternatively spliced, partial cds 3758 8896 13987 1.1 0 A1377699.1 EST HUMAN te62fl 0.xl Soares NFL T GBC S1 Homo sapiens cIDNA done IMAGE:2091307 X 3759 8897 1.7 0 AF152496.1 NT Homo sapiens protocadhedn beta 3 (PCDH- beta3) mRNA complete cds 3760 8898 13988 4.5 0 4758199 NT Homo sapiens desmoplakin (13131, DPII) (DSP) mRNA 3763 8901 13990 11.7 0 S78685.1 NT Homo sapiens ATP-sensifive inwardly rectifying K-channel subunit (WNJ6/BIR1) gene, complete cds 3764 8902 13991 2.0 0 7710148 NT Homo saplens methyl CpG binding protein 2 (MECP2), mRNA 3765 8903 13992 2.4 0 7662183 NT Homo saplens KIAA0569 gene product (K1AA0569), mRNA 3768 8906 13994 1.3 0 AF069601.2 NT Homo sapiens myosin light chain kinase isoform 2 ^CK) mRNA, complete cds 3768 8906 13995 1.3 0 AF069601.2 NT Homo sapiens myosin light chain kinase lsoform 2 (MI-CK) mRNA, complete cds 3769 8907 13996 2,0 0 4504534 NT Homo s ns 5-hydroxytryptamine (scrotonin) receptor I D (HTRI D) mRNA 3773. 8910 13999 1.6 0 AL163279.2 NT Homo sapiens chromosome 21 segment HS21 C079 3775 8912 14002 1.1 0 6912735 NT Homo sapiens transient receptor potential channel 5 (TRPC5), mRNA 3780 8917 14010 5.7 0 4503178 NT Homo sapiens chromosome X open reading frame 5 (CXORF5) mIRNA 3780 8917 14011 5.7 04503178 NT Homo sapiens chromosome X open reading frame 5 (CXORF5) mRNA 3782 8919 14014 3.9 0 U09412.1 NT Human zinc finger protein ZW1 34 mRNA, complete cds 3784 8921 14015 1.6 0 4826783 NT Homo sapiens potassium voltag"ated channel, Shab-related subfamily, member 1 (KCNB1) mRNA 3787 8924 14018 1.0 0 AF012615.1 NT Homo sapiens familial mental retardation protein 2 (FIVIR2) gene, exon 11 3788 8925 14019 1.9 0 4759171 NT Homo saplens SC35-Interacting protein 1 (SRRP129), mIRNA 3790 8927 14021 0.8 0 AF099117.1 NT Homo saple s amphiphysin ene, partial cds 3799 8936 14030 2.5 0 A1864727.1 EST-HUMAN wkOlfOl.xl NCI_PGAP-4m12 Homo saplens cDNA done IMAGE:2411065 Ysimilar to TR:043340 043340 R28830 2.;contains element PTR7 repetitive element 3802 8939 14034 6.1 0 4506742 NT Homo saplens ribosomal protein S8 (RPS8), mRNA 3807 8944 14040. 1.4 0 AL040338.11 EST-HUMAN DKFZp434N0413-rl 434 (synonym: htes3) Homo sapiens cIDNA done DKFZp434NO413 5' 3813 8950 14047 1.0 0 6005887 NT Homo sapiens API gamma subunit binding protein 1 (AP11 GBPl), mRNA 3813 8950 14048 1.0 0 6005887 NT Homo sapiens AP1 gamma subunit binding protein 1 (AP1 GBPl), mRNA 3815 8952 14050 2.5 0 4504138 NT Homo saplens glutamate receptor, metabotropic 3 (GIRM3) mIRNA 3816 8953 1.8 0 4505078 NT Homo sapiens melanoma antigen, family B, 1 (MAGEB1) mRNA 3820 8957 14053 0.9 0 AF149412.1 NT Homo sapiens H13P17 hepadn-binding and FGF-binding protein gene, complete cds 3830 8967 14066 1.3 0 4506758 NT Homo saplens ryanodine receptor 3 (RYR3) mRNA 3833 8970 140691 1.6 0 4585642 NT Homo saplens zinc finger protein (K1AA0412) mRNA /1 ', 1 Page 188 of 196 Table 4

3842 8978 14078 1,4 0 AW888221.1 EST HUMAN IVIXIRA5 Human matrix tissue expression library Homo sapiens cDNA done Incyte 1996726 similar to IVIXIRA5 Matrix remodeling associated gene 5.

3842 8978 14079 1.4 0 AW888221.1 EST HUMAN WRAS Human matrix tissue expression library Homo sapiens cDNA clone Incyte 1996726 similar to MXRAS Matrix remodeling associated gene 5 3848 8984 14084 1.8 0 AF129533.1 NT Homo sapiens F-box protein Fb13b (F81- 313) MRNA, partial cds 3853 8989 14089 3.2 0 BE378602.1 EST-HUMAN 601236966F1 NIH-MGC 44 Homo saplens eDNA done IMAGE:3608800 5' 3854 8990 14090 1.3 0 BE313146.1 EST-HUMAN 601153727F1 NIH_MGC_19 Homo sapiens cDNA done IMAGE:3509743 5' 3860 8996 14095 1.0 0 AWS80740.1 EST-HUMAN PM3-LT0031-100100-003-h09 LT0031 Homo saplens cDNA 3893 W28 14119 4.6 0 AF1 16195.1 NT Homo sapiens cancer-teitis antigen CT1 0 (CT1 0) gene, complete cds 3893 9028 14120 4,6 0 AF116195.1 NT Homo saplens cancer-tesfis antigen CT10 (CTIO) gene, complete ods 3903 9038 3.9 0 M239110.1 NT Human MHC da,4s 11 lymphocyte antigen DIRw4- beta-2 pseudogene, exon 2 3905 9040 5.4 0 AL163303.2 NT Homo sapiens chromosome 21. segment HS21 Cl 03 3912 9047 14135 1.5 0 AL118494.1 NT Novel human gene mapping to chomosome 20 3916 9050 14137 2.9 0 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 3924 9058 14145 1.5 0 A1-163268.2 NT Horno sapiens chromosome 21 segment HS21 C068 3935 9069 25.8 0 4503470 NT Horno sapi ns eukaryodc translation elongation factor 1 alpha 1 (EEFIAI) mIRNA 3939 9072 14155 1.2 0 7662183 NT Homo sapiens KIAA0569 gene product (K1AA0569), mRNA 3940 9073 14156 2.1 0 U09366.1 NT Human zinc finger protein ZNF133 3960 9092 14177 6.2 0 AB015610.1 NT Chforocebus aethiops mRNA for ribosomal protein S4X, complete cds 3968 9100 3.8 0 AJ238617.1. NT Homo sapjens mRNA for UGA suppressor tRNA- associated andgenic protein (tiRNA48 gene) 3976 9108 14190 3.3 0 A.1277276.1 NT Homc sapiens mRNA for rapa-2 (rapa gene) 3976 9108 14191 3.3 0 AJ277276.1 NT Homo saplens mRNA for rapa-2 (rapa gene) 3981 9113 14195 5.6 0 5032026 NT Homc saplens retinoblastoma-binding protein 4 (RBBP4) mRNA 3981 9113 14196 5.6 0 5032026 NT Homo sapiens rednoblastoma-binding protein 4 (R1313P4) mRNA 3993 '9125 14209 0.8 0 4503914 NT Homo sapiens phosphodbosylglycinamide formyltransferase, phosphodbosylgiycinamide synthetase. phosphoribosylaminolmIdazole synthetase (GART) mRNA 4000 9131 14214 4,9 0 4885306 NT Horno sap)ens G protein-coupled receptor 21 (GPR21), mRNA 4ool 9132 14215 1.9 0 A13006625.1 NT Homo sapiens mRNA for KIAA0287 gene, partial cds 4003 9134 14216 1.3 0 4758807 NT Horno saplens ras GTPase activating protein-like (NGAP) mRNA 4005 9136 14218 1.6 0 AL096857.1 NT Novel human mRNA from romosorne 1, which has similarities to BAT2 genes 4013 9144 14227 2.7 0 AF165527.1 NT Homo sapiens DGCR8 (DGCR8) mRNA, complete cds 4017 10299 14229 0.9 0 AF157476.1 NT Homo saplens DNA polymerase zeta catalytic subunit (REV3) mRNA, complete cds 4022 6253 11404 0.8 0 4826947 NT Homo sapiens protein kinase, X-linked (PIRKX) mRNA 4022 6253 11405 0.8 0 4826947 NT Homo sapiens protein kinase, X-finked (PIRKX) mRNA 4028 9156 14239 1.0 0 5901905 NT Horno, sapiens butyrophilin, subfamily 3, member A2 (BTN3A2), mIRNA 4029 9157 14 240 1.2 0 4503854 NT Horno 1 sapiens GA-binding protein transcription factor, alpha subunit (60kID) (GABPA), mRNA 9157 14241 1.2 0 4503854 NT - Homo sapiens GA-binding protein transcription factor, alpha subunit (6OkD) (GABPA), mRNA Page 189 of 196 Table 4

9 r 4032 9160 14243 1.1 0 8922391 NT Homo sapiens hypothetical protein FLJ10379 (FLA0379), mRNA 4032 9160 14244 1.1 0 8922391 NT Homo saplens hypothetical protein FLJ10379 (FLJ10379), mRNA 4041 9169 14254 4.6 0 A1982597,1 EST HUMAN wu04d04.xl NCI CGAP GC6 Homo sapiens cDNA done IMAGE:2515975 3, 4041 9169 14255 4.6 0 A1982597.1 EST HUMAN wu04d04.xl NCI CGAF_GC6 Horno sapiens cIDNA clone IMAGE:2515975 3' 4044 9171 14257 0.9 0 BE184856.1 EST-HUMAN MR11-HT0707-100500-001-a02 HT0707 Homo sapiens cDNA 4044 9171 14258 0.9 0 BE184856.1 EST HUMAN MR1-HT0707-100,500-001-a02 HT0707 Homo saplens cDNA 4048 9175 2.0 0 BE274217.1 EST HUMAN 601 120778F1 NJK_MG_20 Homo sapiens cIDNA done IMAGE:2967690 5, 4055 9182 14265 1.0 0 4507476 NT Homo saplens transgiutaminase 3 (E polypepfide, protein-glutamine-gamma glutamyitransferase) (TGM3) mRNA 4056 9183 14266 2.0 0 5729725 NT Homo saplens nuclear receptor coactivator 3 (NCOA3), mRNA 4064 9191 6.2 0 AW675599.1 EST HUMAN ba51f04.xl NIH-MGC-10 Homo sapiens cDNA clone IMAGE:2900095 Ysimilar to SW:TH12-BOVIN Q95108 MITOCHONDRIAL THfOREDOXIN PRECURSOR; 4069 9196 14280 1.5 0 AW408788.1 EST HUMAN U]-HF-BMO-adx-c-02-0-Ul.rl NIH- MGC-38 Horno sapiens cIDNA clone IMAGE.3063147 V 4071 9198 14283 1.3 0 8922466 NT Homo sapiens hypothetical protein FLJ10498 (FLA0498), mIRNA 4071 9198 14284 1.3 0 8922466 NT Homo sapiens hypothetical protein FLJ10498 (FLA0498), mRNA 4081 9208 2,0 0 5174632 NT Homo sapiens polycysfic kidney disease (polycysfin) and REJ (sperm receptor for egg jelly.

sea urchin homolog)41ke (PKDREJ) mRNA 4098 9224 14307 7.8 0 AA401438.1 EST-HUMAN zu68h07.sl Soares-tesbp_NHT Homo sapiens cDNA done IMAGE:743197 3' similar to contains Alu repetitive element;contalns element MER35 repetitive element:

4098 9224 14308 7.8 0 AA401438.1 EST-HUMAN zu68h07.sl Soares-testis_NHT Homo sapiens cDNA done IMAGE:743197 Ysimflar to contains Alu repetitive element;contains element MER35 repetitive element:

4113 9239 14323 3.8 0 4507720 NT Homo sapiens tifin (TTN) mRNA 4113 9239 14324 3.8 0 4507720 NT Homo sapiens fitin (TTN) mRNA 4126 9252 14333 0.9 0 4506882 NT Homo saplens semenogelin 1 (SEIVIG1) mRNA 4130 9256 14335 8.2 0 4758199 NT Homo sapiens desmoplakin (DPI, DIP11) (DSP) mRNA 4130 9256 14336 8.2 0 4758199 NT Horno sapiens desmoplakin (DPI, DPII) (OSP) mRNA 4137 9263 0.9 0 AL163303.2 NT Homo sapiens chromosome 21 segment HS21 Cl 03 4163 9289 14369 1.1 0 AJ003145.1 NT Homo saplens mRNA for olfactory receptor protein, pseudogene 4176 9302 14383 7.8 0 J02610.1 NT Human apolipoprotein B-100 mRNA, complete cds 4190 9316 14400 1.0 0 AW936689.1 EST-HUMAN PM2-DT0023-080300-004a08 DT0023 Homo saplens cDNA 4196 9322 14408 0.9 0 4826827 NT Homo sapiens myelodysplasla syndrome 1 (MDS1) mRNA 4196 9322 14409 0.9 0 4826827 NT Homo sapiens myelodysplasia syndrome 1 (MDS1) mRNA 4198 9324 14411 5.7 0 AF174590.1 NT Homo saplens F-box protein Fb14 (F131- 4) mRNA, partial cds 4206 9331 1.9 0 A1189844.1 EST-HUMAN qd23f06.xl SoareMiacenta-8togweeks- 2NbHP8togW Homo sapiens cIDNA clone IMAGE:1724579 31 similar to contains MER2012 MER20 repetitive element; 4210 9334 4. 1 0 u14520,1 NT Human CBFA3 (Cbfa3) gene, partial cds 4224 9348 14429 1.0 0 4505646 NT Homo saplens proprotein convertase subdilsinlke)dn type 2 (PCSK2) mRNA 4230 9354 14436 0.9 0 6563384 NT Homo saplens protein kinase C, nu (PRI(CN), mRNA 4230 93 14437 0.9 0 6563384 NT Homo saplens protein kinase C, nu (PRKCN), mRNA 939 42 61 14443 1.2 0 U10991.1 NT Human G2 protein mRNA, partial cds 1 Page 190 of 196 Table 4

4237 9361 14444 1.2 0 U10991.11 NT Human G2 protein mRNA, partial cds 4245 9370 14449 10.2 0 6912281 NT Homo saplens COMPLEMENT COMPONENT Clq RECEPTOR (Cl QR), mRNA 4263 9388 1.2 0 AF153047.2 NT Homo sapiens gap)unction protein conne)dn- 36 (CX36) gene, complete cds 4268 '9393 14475 1.6 0 U03901,1 NT Human lg light chain V1.1 region germline (humivl c2c) gene, partial cds 4274 9399 14482 5.2 0 L14561.1 NT Homo sapiens plasma membrane'calclum ATPase isoform 1 (ATP2131) gene, alternative splice products, partial cds 4278 9403 14487 2.8 0 Z80780.1 NT H.sapiens H 131h gene 4278 9403 14488 2.8 0 Z80780.1 NT H.sapiens H2131h gene 4284 9409 14494 1.6 0 X60483.1 NT H.sapiens H4/d gene for H4 histone 4284 9409 14495 1.6 0 X60483.1 NT H.saplens H4/d gene for H4 histone 4289 9413 14W1 9.0 0 7662091 NT Homo sapiens KIAA0390 gene product (K1AA0390), rnRNA 4289 9413 14502 9.0 0 7662091 NT Homo sapiens KIAA0390 gene product (K1AA0390), rnRNA 4299 9423 14514 10.8 0 4885126 NT Homo saplens caudal type homeo box transcription factor 4 (CDX4), mRNA 4302 9426 14516 1.0 0 AB037781.1 NT Homo sapiens mRNA for KIAA1360 protein, pardaicds 4333 9.456 14544 1.1 0 7Q19456 NT Homo saplens myosin regulatory light chain Interacting protein (MIR), mRNA 4346 9469 14557 8.1 0 AJ249765.1 NT Homo sapiens ACTN2 gene for alpha- Acdnin 2, exon 10 4346 9469 14558 8.1 0 AJ249765.1 NT Homo sapiens ACTN2 gene for alpha- Actinin 2, exon 10 4368 9490 1,9 0 AF200629.1 NT Homo sapiens HPS1 gene, Intron 5 4401 9522 14612 40.2 0 AW084964.1 EST HUMAN xc68e08.xl NCI. CGAP - Eso2 Horno saplens cDNA done IMAGE:2589446 Ysimilar to SW:AHNK-HUMAN Q09666 NEUROBLAST DIFFERENTIATION ASSOCIATED PROTEIN AHNAK; 4403 10301 1.9 0 8051619 NT Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a,mRNA 4405 9525 14615 1.4 0 AF016050.1 NT Homo sapiens vascular endothelial cell growth factor 165 receptor/neuropilin (VEGFl 65) mRNA, complete cds 4408 9528 7.5 0 AL163207.2 NT Homo sapiens chromosome 21 segment HS21 C007 4410 9530 14619 1.3 0 AW381570.1 EST HUMAN PM1-HT0305-101199-002-d03 HT0305 Homo sapiens cDNA 4416 9536 14626 1,8 0 AJ278120.1 NT Homo sapiens mIRNA for putative ankyrin7T at containing p tein (ORF1) 4416 9536 14627 1.8 0 AJ278120.1 NT Homo saplens mRNA for putative ankyrin-rep a- - - - inidg protein (ORF1) 4418 9538 14629 3.9 0 4758467 NT Homo sapiens G protelln-coupled receptor 50 (GPR50) mRNA 4419 9539 14630 2.3 0 AF108830.1 NT Horno saplens sedne-threonine protein kinase (MNSH) mRNA, complete cds 4424 9544 14636 1.5 0 Z66526.1 NT H.sapiens pancreatic polypepude receptor PP1 gene 4429 9550 14643 1.1 0 S78684.1 NT Homo sapiens ATP-sensifive Inwardly. rectifying K-channel subunit (KCNJ6/BIR1) gene, exon 4430 9551 14644 2.0 0 AF111163.1 NT Horno sapiens pyrin (MEFV) gene, complete cds 4430 9551 14645 2.0 0 AF1 11163.1 NT Homo sapiens pyrin (MEFV) gene, complete ods 4439 10302 14655 3.1 0 6005973 NT Horno sapiens 7Jnc finger protein 195 (ZNFI95), mRNA 4444 9564 14660 5.4 0 AF208161.1 NT Horno saple s syncytin precursor, mRNA, complete cds 4449 9569 14667 1.9 0 AF152337.1 NT Homo saplens protocadherin gamma C3 (PCDH-garnma-C3) mRNA, complete ods 4452 9572 1.4 0 5454175 NT Homo sapiens Anc finger protein 211 (ZW21 1), mRNA 4462 9582 14679 15.2 0 4503470 NT Homo sapiens eukaryofic translation elongation factor 1 alpha 1 (EEF1A1) mRNA (7,11 Page 191 of 196 t Table 4

C- 1 4473 9593 14688 1.5 0 4503098 NT Homo sapiens chondroifin sulfate proteoglycan 4 (metanoma-associated) (CSPG4), MRNA 4477 9597 14693 1.6 0 4502556 NT Homo sapiens calcium/calmodulin- dependent protein kinase IV (CAMK4) mIRNA 4481 9601 2.8 t.9 1.35485.1 NT Homo sapiens iduronate sulphate sulphatase (IDS) gene, complete ods 4483 9603 14695 9.8 0 7662091 NT Homo saplens KIAA0390 gene product (K1AA0390), mRNA 4483 9603 14696 9.8 0 7662091 NT Homo sapiens KIAA0390 gene product (K1AA0390), mRNA 4498 9618 14709 2.0 0 AF143314.1 NT Homo sapiens PTEN (PTEN) gene, exons 3 through 5 4500 9620 14711 8.7 0 AJ245418.1 NT Homo sapiens mIRNA for G7c protein (G7c gene located in the class Ill region of the major histocompatibility complex) 4500 9620 14712 8.7 0 AJ245418.1 NT Homo sapiens mIRNA for G7c protein (G7c gene located in the class lit region of the major histocompatibility complex) 4511 9630 0.8 0 D87675.1 NT Homo sapiens DNA for amytold precursor protein, complere -cds 4523 9642 1.5 0 AA174072.1 EST HUMAN zp18908.sl Stratagene fetal retina 937202 Homo sapiens cDNA done IMAGE:609854 X 4526 9645 1.6 0 7657410 NT Homo sapiens odz (odd OzAen-m, Drosophila) homolog 1 (ODZ1), mRNA 4528 9647 1.2 0 AL163284.2 NT Homo sapiens chromosome 21 segment HS21 C084 4529 9648 14737 1.7 0 AF1 84110.1 NT Homo sapiens cyclophilin- lated protein (NKTIR) gene, complete cds 4530 9645 14738 4.4 0 AL163300.2 NT Homo sapiens chromosome 21 segment HS21 Cl 00 4531 9650 1.9 0 A13037521.1 NT Homo sapiens gene for natriuretic protein, partial cds 4541 9660 14748 1.9 0 4557887 NT Horno sapiens keratin 18 (KIRT18) mRNA 4541 9660 14749 1.9 0 4557887 NT Homo sapiens keratin 18 (KIRT18) mIRNA 4542 9661 14750 1.3 0 AF153819.1 NT Horno, sapiens inwardly-rectifying potassium channel Kir2.1 (KCNJ2) gene, exon 2 and complete cds 4542 9661 14751 1.3 0 AF153819.1 NT Homo sapiens inwardly-rectifying potassium channel Kir2.1 (KCNJ2) gene, exon 2 and complete cds 4554 9239 14323 7,4 0 4507720 NT Homo sapiens tifin (TTN) mRNA 4554 9239 14324 7.4 0 4507720 NT Homo saplens tifin (TTN) mRNA 4560 9678 14770 22.0 0 Y18890.1 NT Human endogenous retrovirus type K (HERV-K), gag. pol and env genes 4566 9684 14778 2.3 0 BE081527.1 EST-HUMAN QV2-BT0635-160400-142-h05 BT0635 Homo sapiens cDNA 4574 9691 2.0 0 AF086641.1 NT Homo sapiens truncated tenascin X13 (TNXB) gene, partial ods and TWA gene recombination breakpoint region 4580 9696 14789 2.7 0 AB037820.1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 4580 9696 14790 2.7 0 AB037820.1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 4581 9697 14791 2.6 0 M74099.1 NT Human displacement protein (CCAAT) mIRNA 4585 9700 14794 1.8 0 6453812 NT Homo sapiens butyrophilin, subfamily 2, member A2 (BTN2A2), mRNA 4585 9700 14795 1.8 0 6453812 NT Homo sapiens butyrophilin, subfamily 2, member A2 (BTN2A2), mRNA 4586 5330 10461 1.0 0 T56945.1 EST HUMAN ya83g0C2 Stratagene feta. 1 spleen (#937205) Homo saplens cIDNA clone IMAGE:68310 5, 4586 5330 10462 1.0 0 T56945.1 EST HUMAN ya83g0C2 Stratagene fetal spleen (#937205) Homo saplens cDNA clone IMAGE:68310 5 9701 1.0 0 BE278730.1 EST HUMAN G 601158935F1 N1H MGQ 21 Homo sapiens cIDNA clone IMA E:3505521 5' 1 1 Page 192 of 196 Table 4

C1r) (>IZ 0 4593 9707 14799 1A 0 BE390050.1 EST HUMAN 601285246F1 N1H MGQ_44 Homo sapiens cDNA clone IMAGE:3607067 6 4614 9728 14822 37.4 0 M80902,1 NT Human AHNAK nudeoprotein mRNA, Vend 4617 9731 14825 3,1 0 M69197.1 NT Human haptoglobin and haptoglobin- related protein (HP and HPR) genes, complete cds 4617 9731 14826 3.1 0 M69197.1 NT Human haptoglobin and haptoglobin- related protein (HP and HPR) genes, complete ods 4620 9734 14829 1.1 0 AF184110.1 NT Homo sapiens cycJophilin-retated protein (NKTR) gene, complete cds 4621 9735 14830 1.3 0 7662181 NT Homo saplens KIAA0563 gene product (K1AA0563), mRNA 4636 9750 1.5 0 X58467.1 NT Human CYP2D7AP pseudogene for cytochrome,P450 2D6 4644 9758 14851 1.0 0 7304922 NT Homo sapiens bromodomain adjacent to zinc finger domain, 2B (BAZ2B), mRNA 4644 9758 14852 1,0 0 7304922 NT Homo sapiens bromodomain adjacent to zinc finger domain, 213 (BAZ2B), mRNA 4652 9764 14859 1.1 0 AF026801.1 NT Homo sapiens alpha-3 type IX collagen (COL9A3) gene. promoter region, and exons 1-26 4655 9767 14862 0.9 0 7019320 NT Homo sapiens proteinx0008 (AD013), mRNA 4655 9767 14863 0.9 0 7019320 NT Homo sapiens protelnx0008 (AD013), mRNA 4676 9788 14887 1.8 0 AW444637.1 EST HUMAN Ul-H-B13-ajw-c-04-0-Ul.sl NCI- CGAP-Sub5 Homo sapiens cDNA clone IMAGE:2733294 3' 4686 9798 1.8 0 AF083242.1 NT Homo sapiens I-ISPC024-iso mRNA, complete cds 4697 9809 2.0 0 M65189.1 NT Human connexin 43 processed pseudogene 4737 9848 14940 2.8 0 X87205.1 NT M.fascicularis mRNA for metalloprotease- like, disintegrin-like protein, 1Va 4739 9850 14942 1.3 0 AF084479.1 NT Homo sapiens Williams-Beuren syndrome deletion transcript 9 (Wi3SCR9) mRNA, complete cds 4740 9851 14943 1.5 0 AF097416.1 NT Mus musculus zinc finger transcription factor Kaiso mRNA, complete cds 4741 9852 14944 3.5 0 4503766 NT Homo sapiens fragile X mental retardation 2 (FMR2) mRNA 4743 9854 14946 61.8 0 4885048 NT Homo sapiens actin, alpha, cardiac muscle (ACTC), mRNA 4744 9855 14947 1.4 0 P52740 SWISSPROT ZINC FINGER PROTEIN 132 4746 9857 14949 1.0 0 8922180 NT Homo sapiens hypothetical protein DKIZZp762E1 312 (1)KFZp762E1 312), mRNA 4748 9859 14951 0 8 0 AL163203.2 NT Homo sapiens chromosome 21 segment HS21C003 4752 9863 14956 7.8 0 8923080 NT Homo sapiens hypothetical protein FLJ20073 (FU20073), mRNA 4756 9867 14960 1.0 0 7661979 NT Homo sapiens KIAA01 87 gene product (K1AA01 87), mRNA 4757 9868 14961 1.7 0 M94081.1 NT Human Tcr-C-delta gene, exons 14; Tcr-V- delta gene, exons 1-2; T-cell receptor alpha (Tcr alpha) gene, J1-J61 segments; and Tcr-C-alpha gene, exons 1-4 4757 9868 14962 1.7 0 M94081.1 NT Human Tcr-C-delta gene, exons 14; Tcr-Vdelta gene, exons 1-2; T-cell receptor alpha (Tcr alpha) gene, J1-M segments; and Tcr-C-alpha gene, exons 1-4 4759 9870 14964 1.7 0 X94628.1 NT H.sapiens MeCP-2 gene 4759 9870 14965 1.7 0 X94628.1 NT H.sapiens MeCIP-2 gene 4762 9873 14968 1.1 0 M55582.1 NT Human collagenase type IV (CLG4) gene, exon 2 4763 9874 14969 3,2 0 AL163280.2 NT Homo sapiens chromosome 21 segment HS21C080 4776 9886 14979 1.0 0 5032150 NT Homo saplens TATA box binding protein (TBPassociated factor, RNA polymerase 11, 1, 28ki) (TAF21) mRNA 4786 9897 14990 0.8 0 6806918 NT Homo saplens low density lipoprotein- related protein 2 (LRP2), mRNA 4788 9899 14992 1.4 0 X92841.1 NT H.sapiens MICA gene 4791 9902 14995 2.0 0 4585642 NT Homo sapiens zinc finger protein (K1AA0412) mRNA Page 193 of 196 Table 4

J_ C110 C 4792 9903 14996 1.2 0 AB014533.1 NY Homo saplens mRNA for KIAA0633 protein, partial cds 4794 9905 14998 2.3 0 6677648 NT Mus musculus zinc finger protein Interacting with K protein 1 (Zfkl), mRNA 4795 9906 14999 1.1 0 5174560 NT Homo sapiens meningloma expressed andgen 6 (coiled-coil proline-rich) (MGEA6), mIRNA 4797 9908 15001 8.6 0 4758199 NT Homo sapiens desmoplakin (DIRI, DPI1) (DSP) mRNA 4799 9910 15003 1.2 0 Y16723.1 NT Homo sapiens gene encoding filensin, exon 8 4800 9911 15004 1.6 0 7705546 NT Homo sapjens zinc-finger DNA-binding protein (HUMHOXY1), mRNA 4801 9912 1,3 0 AJ010442.1 NT Homo sapiens mIRNA for immunoglobutin kappa light chain, anfi-RhD, therad 7 4806 9916 15009 24.9 0 AF055066.1 NT Homo saplens WC class 1 region 4808 9918 2.4 0 4505508 NT Homo sapiens opiold receptor, delta 1 (OPRDI) mIRNA 4809 9919 15012 2.5 0 AF091 711.1 NT Homo sapiens sEfice vadant AKAP350 mRNA, partial cds 4812 9239 14323 5.5 0 4507720 NT Homo saplens fitin (TTN) mRNA 4812 9239 14324 5.5 0 4507720 NT Homo sapiens tifin (TTN) mRNA 4814 9923 15016 0.9 0 AJ277892.1 NT Horno sapiens partial TTN gene for Udn 4824 9932 15022 12.0 0 4507720 NT Homo saplens fifin (TTN) mRNA 4827 9935 15025 1.0 0 D63562.1 NT Homo sapiens COL4A6 gene for a6(1V) collagen, exon 44 and partial cds 4831 9939 15028 1.5 0 4503684 NT Homo sapiens famesyl diphosphate synthase (famosyl pyrophosphate synthetase, dimethylailyitranstransferase, geranyltranstransfera.5c) (FDPS) mIRNA 4837 9545 14637 1,1 0 4506952 NT Homo saplens slalyltransferase 8 (alpha- N-acetyincuraminate: alpha-2,8-sialytransferase, GD3 synthase) (SIAT8) mIRNA 4845 9952 15040 1.3 0 D15050.1 NT Human mIRNA. for transcription factor AREB6, complete eds 4846 0962 15041 1.3 0 D15050.11 NT Human mIRNA for transcription factor ARE86, complete cds 4854 9961 15047 0.9 0 A13026898.1 NT Homo sapjens DNA, DLECI to ORCTIL4 gene region, section 112 (DLEC1, ORCTIL3, ORCTL4 genes, complete cds) 4871 9978 15062 1.3 0 AL163284.2 NT Homo sapiens chromosome 21 segment HS21 C084 4879 9986 15068 1.5 0 AW452728.1 EST-HUMAN U[-H-B13-aiv-f-02-0-Ul.sl NCI- C_SubS Homo sapiens cDNA done IMAGE:3068691 3, 4885 9993 15074 1.2 0 8922926 NT Homo sapiens hypothetical protein FUll 190 (FU11190), mIRNA 4886 9932 15022 8.0 0 4507720 NT Homo sapiens tifin (TTN) mRNA 4888 9995 15076 2.8 0 AF058332.1 NT Homo sapiens titin (TTN) gene, alternative splice products, pardal cds 4888 9995 15077 2.8 0 AF058332.1 NT Homo sapiens fitin (TTN) gene, alternative splice products, partial ods 4894 10001 15084 3.0 0 4507720 NT Homo saplens tifin (TTN) mRNA 4897 10004 4.3 0 U14967.1 NT Human ribosomal protein L21 mRNA, complete cds 4910 10017 2.6 0 SE408863.1 EST HUMAN 601303729F1 N1H MGC 21 Horno saplens cIDNA clone IMAGE:3638118 5' 4915 10022 15101 5.4 0 4758199 NT Homo sapiens desmoplakin (DPI. DPI1) (DSP) mIRNA.

* 4925 10032 15106 1.0 0 AB028966.1 NT Horno saplens mRNA for KIAA1043 protein. partial cd.9 4939 10045 15116 1.7 0 8923441 NT Homo saplens hypothetical protein FLJ20477 (FU20477). rnRNA iAHomo 4939 10045 15117 1.7 0 8923441 NT sapiens hypothetical protein FLJ20477 (FU20477), mIRNA Page 194 of 196 1 Table 4

W C"1 4953 10058 15128 1.1 0 U82671.2 NT Homo sapiens chromosome Xq28 melanoma antigen family A2a (MAGEA2A), melanoma antigen family A12 (MAGEA12), melanoma antigen family A2b (MAGEA2B), melanoma antigen family A3 (MAGEA3), caltractin (CALT), NAD(P)H dehydrogenase-like protein (NSDHL), and Ll> 4953 10058 15129 1.1 0 U82671.2 NT Homo sapiens chromosome Xq28 melanoma antigen family A2a (MAGEA2A), melanoma antigen family A12 (MAGEA12), melanoma antigen family A2b (MAGEA2B), melanoma antigen family A3 (MAGEA3), caltractin (CALT), NAD(P)H dehydrogenase-like protein (NSDHL), and Ll> 4957 9239 14323 5,8 0 4507720 NT Homo sapiens fitin (TTN) mRNA 4957 9239 14324 5.8 0 4507720 NT Homo saplens fitin (TTN) mRNA 4959 10062 15134 3.5 0 4507720 NT Homo sapiens fitin (TTN) mRNA 4960 10063 15135 7.8 0 4507720 NT Homo sapiens titin (TTN) mRNA 4968 10071 1.2 0 4758225 NT Homo sapiens E2F transcription factor 2 (E2F2) mRNA 4978 10081 15151 1,4 0 AF016705.1 NT Homo sapiens E6-AP ubiquign-protein ligase (UBE3A) gene, exon 3 4987 10090 1.3 0 AL163209.2 NT Homo saplens chromosome 21 segment HS21C009 4990 10093 37.8 0 D50657.1 NT Homo sapiens gammma-cytoRtasmic actin (ACTGP3) pseudogene 5000 10103 15171 2.3 0 AJ277892.1 NT Homo sapiens partial TTN gene for fitin W01 10104 15172 3.0 0 4507720 NT Homo saplens titin (TTN) mRNA 5003 10106 15174 4.2 0 4507720 NT Homo sapiens fifin (TTN) mRNA 5005 9239 14323 2.9 0 4507720 NT Homo saplens fifin (TTN) mRNA 5005 9239 14324 2.9 0 T507720 NT Homc, saplens fitin (TTN) mRNA 5018 10120 15192 2.4.0 X52988.1 NT Bacillus amAoliquefadens sacB gene for levansucrase (EC 2.4.1.10) 5037 10137 15213 1.8 0 AF240635.1 NT Homo sapiens vascular endothelial cadherin 2 mRNA, complete cds 5037 10137 15214 1.8 0 AF240635.1 NT Homo sapiens vascular endothelial cadherin 2 mRNA, complete cds 5040 10140 15218 1.0 0 5454153 NT Homo sapiens cyclophilin (USA-CYP) mRNA 5054 10154 15230 1.2 0 6677700 NT Homo, saplens G-protein coupled receptor (RE2), mRNA 5055 9239 14323 11.2 0 4507720 NT Homo sapiens fifin (TTN) mRNA 5055 9239 14324 11.2 0 4507720 NT Homo sapiens tifin (TTN) mRNA 5056 9239 14323 14.9 0 4507720 NT Homo sapiens tifin (TTN) mRNA 5056 9239 14324 14.9 0 4507720 NT Homo sapiens fitin (TTN) mRNA 5065 10163 15240 1.3 0 4557362 NT Horno sapiens PR domain containing 1, with ZNF domain (PRDMI) mRNA 5070 10168 15245 1.0 0 M10905.1 NT Human cellular fibronectin mRNA 5070 10168 15246 1.0 0 M10905.1 NT Human cellular fibronectin mRNA 5071 10169 15247 1.1 0 U91328.1 NT Human hereditary haemochroniatosis region, histone 2A-like protein gene, hereditary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete ods 5080 10178 15258 3.0 0 4507720 NT Homo sapiens tifin (TTN) mRNA 5096 9239 14323 16.3 0 4507720 NT Homo saplens fitin (TTN) mRNA 5096 9239 14324 6.3 0 4507720 NT U- saplens dfin (TTN) mRNA t 5098 10195 15275 1.3 0 L35475.1 NT ictory receptor-like gene, complete cds 15276 1.3 0 ictory receptor-like gene, complete cds Page 195 of 196 Table 4

5099 9239 14323 9.4 0 4507720 NT Homo saplens Efin (TTN) mRNA 5099 9239 14324 9.4 0 4W7720 NT Homo sapiens fitin (TTN) mRNA 5124 10220 15297 0,9 0 AF195658.1 NT Homo sapiens DNA mismatch repair protein (M1-H3) gene, complete cds 5126 10221 15298 1.4 0 5360213 NT Homo sapie5s glypican 3 (GPC3) mRNA 5130 10225 15300 0.8 0 AE000327.1 NT Eschedchia coli K-12 MG1655 section 217 of 400 of the complete genome 5140 10235 15310 1.1 0 4885474 NT Homo saplens melanoma antigen, family C, 1 (MAGEC1), mRNA 5159 10254 15325 1.0 0 4885474 NT Homo sapiens melanoma antigen, family C, 1 (MAGEC1), mRNA 5162 10256 15326 1.6 0 4758697 NT Homo sapiens mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA 5165 10259 15329 1.1 0 AF245703.1 NT Homo sapiens toll-like receptor 8 (TLRB) mRNA, complete cds 5165 10259 15330 1.1 0 AF245703.1 NT Homo sapiens toll-like receptor 8 (TLR8) mRNA, complete cds 5186 10278 15346 1.7 0 AF006061.1 NT Homo sapiens placental growth hormone Isoform hGH-W (hGH-V) mRNA, complete cds 5187 9239 14323 10.4 0 4507720 NT Homo saplens fifin (TTN) mRNA 5187 9239 14324 10.4 0 4507720 NT Horno sapiens titin (TTN) mRNA 5189 10280 15348 6.8 0 4507720 NT Homo sapiens fifin (TTN) mRNA 5193 10284 1.4 0 AL163285.2 NT Homo sa lens chromosome 21 segment HS21 C085 5195 10286 15354 4.0 0 4507720 NT Homo sapiens titin (TTN) mRNA 5195 10286 15355 4.0 0 4507720 NT Homo sapiens tifin (TTN) mRNA 5196 1 15356 1.0 0 4502398 NT Horno sapiens beaded filament structural protein 1, filensin (I3FSP1) mRNA Page 196 of 196 287

Claims (40)

1. A spat i al ly- addres sable set of single exon nucleic acid probes for measuring gene expression in a sample derived from human heart comprising a nucleotide sequence as set out in any of SEQ ID NOS.: 286, 287, 442, 871, 1029, 1280, 1597, 1619, 1694, 1715, 1721, 1848, 1935, 2119, 2893, 3003, 3003, 3149, 3403, 3471, 3513, 3607, 4730, 4779, 4976, 4988, 6723, 6745, 6818, 6838, 8545, 9889, 2233, 4102, 5473, 6844, 4164, 5608, 6966, 4184, 6019, 7052, 8612, 8654, 8746, 10079 and 10091.

4184, 4242, 6169, 6410, 7230, 7340, 8044, 8155, 8298, 9228, 9290, 9310, 9367, 9842,

2. A single exon nucleic acid probe for measuring gene expression in a sample derived from human comprising a nucleotide sequence as set out in any of SEQ ID N0s.: 286, 287, 442, 871, 1029, 1280, 1597, 1619, 1694, 1715, 1721, 1848, 1935, 2119, 2233, 2893, 3003, 3003, 3149, 3403, 3471, 3513, 3607, 4102, 4164, 4184, 4184, 4242, 4730, 4779, 4976, 4988 or a complementary sequence or a unique fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid molecule expressed in the human heart.

human heart

3.

A single exon nucleic acid probe as claimed in claim 2 comprising a nucleotide sequence as set out in any of SEQ ID N0s.: 5473, 5608, 6019, 6169, 6410, 6723, 6745, 6818, 6838, 6844, 6966, 7052, 7230, 7340, 8044, 8155, 8298, 8545, 8612, 8654, 8746, 9228, 9290, 9310, 9367, 9842, 9889, 10079 and 10091 or a complementary sequence or a unique fragment thereof.

288

4. A single exon nucleic acid probe f or measuring human gene expression in a sample derived from human heart which is a nucleic acid molecule having a sequence encoding a peptide comprising a peptide sequence as set out in any of SEQ ID N0s.: 10603, 10741, 11179, 11571, 11893, 11913, 11999, 12017, 12023, 12152, 12245, 12439, 12552, 13144, 13255, 13256, 13407, 13653, 13724, 13841, 14313, 14370, 14390, 14391, 14934, 15149 and 15161, or a complementary sequence or a unique fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human heart.

5. A single exon nucleic acid probe as claimed in any one of claims 1 to 4 wherein said single exon nucleic acid probe comprises between 15 and 25 contiguous nucleotides of said SEQ ID NO.

6. A single exon nucleic acid probe as claimed in any one of claims 1 to 4, wherein said probe is between 3 - 25 20 kb in length.

7. A single exon nucleic acid probe as claimed in any one of claims 1 to 6, wherein said probe is DNA, RNA or PNA.

8. A single exon nucleic acid probe as claimed in any one of claims 1 to 7, wherein said probe is detectably labeled.

9. A single exon nucleic acid probe as claimed in any one 30 of claims 1 to 8, wherein said probe lacks prokaryotic and bacteriophage vector sequence.

289

10. A single exon nucleic acid probe as claimed in any one of claims 1 to 9, wherein said probe lacks homopolymeric stretches of A or T.

6818, 8298, 9842,

11. A nucleic acid sequence as set out NOs: 286, 287, 442, 871, 1029, 1280, 1715, 1721, 1848, 1935 ' 2119, 2233, 3149, 3403, 3471, 3513r 4242, 4730, 4779, 4976, 6410, 6723, 6745, 7340, 8044, 8155, 9290, 9310, 9367, encodes a peptide.

3607, 4102, 4988, 5473, 6838, 6844, 8545, 8612, 9889, 10075 in any of 1597, 28-93, 4164, 5608, 6966, 8654, and 1619, 3003, 4184, 6019, 7052, 8746, 10091 SEQ ID 1694, 3003, 4184, 6169, 7230, 9228, which

12. A peptide encoded by a sequence as set out in any of SEQ ID Nos: 286, 287, 442, 871, 1029, 1280, 1597,1619, 1694, 1715, 1721, 1848, 1935, 2119, 2233, 2893, 3003, 3003, 3149, 3403, 3471, 3513, 3607, 4102, 4164, 4184, 4184, 4242, 4730, 4779, 4976, 4988, 5473, 5608, 6019, 6169, 6410, 6723, 6745, 6818, 6838, 6844, 6966, 7052, 7230, 7340, 8044, 8155, 8298, 8545, 8612, 8654, 8746, 9228, 9290, 9310, 9367, 9842, 9889, 10079 and 10091.

13. A peptide comprising a sequence as set out in any of SEQ ID NOS.: 10603, 10741, 11179, 11571, 11893,11913, 11999, 12017, 12023, 12152, 12245, 12439, 12552, 13144, 13255, 13256, 13407, 13653, 13724, 13841, 14313, 14370, 14390, 14391, 14934, 15149 and 15161.

14. A spatially- addressable set of single exon nucleic acid probes for measuring gene expression in a sample derived from human heart comprising a plurality single exon nucleic probes, said probes comprising any one of the nucleotide sequences set out in SEQ ID NOs: 1 - 5,202 or 290 a complementary sequence, sequence.

or a portion of such a

15. A spatial ly-addressable set of single exon nucleic acid probes as claimed in claim 1 wherein each of said plurality of probes is separately and addressably amplifiable.

16. A spat i al ly- addres sable set of single exon nucleic acid 10 probes as claimed in claim 1 wherein each of said plurality of probes is separately and addressably isolatable from said plurality.

17. A spatial ly-addressable set of single exon nucleic acid 15 probes as claimed in any of claims 1 to 3 wherein said probes comprise any one of the nucleotide sequences set out in SEQ ID NOS.: 5,203 - 10,283.

18. A spat ially-addressable set of single exon nucleic acid 20 probes as claimed in any of claims 1 to 4, wherein each of said plurality of probes is amplifiable using at least one common primer.

19. A spat ially-addressable set of single exon nucleic acid 25 probes as claimed in any of claims 1 to 5 wherein the set comprises between 50 - 20, 000 single exon nucleic acid probes.

20. A spat ially-addressable set of single exon nucleic acid 30 probes as claimed in any of claims 1 to 6, wherein the average length of the single exon nucleic acid probes is between 200 and 500 bp.

21. A spat ially-addressable set of single exon nucleic acid probes as claimed in any of claims 1 to 7, wherein at 291 least 50% of said single exon nucleic acid probes lack prokaryotic and bacteriophage vector sequence.

22. A spatially-addressable set of single exon nucleic acid probes as claimed in any of claims 1 to 8, wherein at least 50% of said single exon nucleic acid probes lack homopolymeric stretches of A or T.

23. A spat ially-addressable set of single exon nucleic acid probes as claimed in any of claims 1 - 9 characterised in that said set of probes is addressably disposed upon a substrate.

24. A spatially-addressable set of single exon nucleic acid probes as claimed in claim 10 wherein said substrate is selected from glass, amorphous silicon, crystalline silicon and plastic.

25. A microarray comprising a spatially addressable set of single exon nucleic acid probes as claimed in any of claims 1 - 11.

26. A single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart comprising a nucleotide sequence as set out in any of SEQ ID N0s.: 1 - 5,202 or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid molecule expressed in the human heart.

27. A single exon nucleic acid probe as claimed in claim 13 comprising a nucleotide sequence as set out in any of SEQ ID N0s.: 5, 203 - 10 0,283 or a complementary sequence or a fragment thereof.

292

28. A single exon nucleic acid probe for measuring human gene expression in a sample derived from human heart which is a nucleic acid molecule having a sequence encoding a peptide comprising a peptide sequence as set out in any of SEQ ID N0s.: 10,284 - 15,322, or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human heart.

29. A single exon nucleic acid probe as claimed in any one of claims 13 to 15 wherein said single exon nucleic acid probe comprises between 15 and 25 contiguous nucleotides of said SEQ ID NO.

30. A single exon nucleic acid probe as claimed in any one of claims 13 to 15, wherein said probe is between 3 - 25 kb in length.

31. A single exon nucleic acid probe as claimed in any one 20 of claims 13 - 17, wherein said probe is DNA, RNA or PNA.

32. A single exon nucleic acid probe as claimed in any one of claims 13 18, wherein said probe is detectably labeled.

33. A single exon nucleic acid probe as claimed in any one of claims 13 19, wherein said probe lacks prokaryotic and bacteriophage vector sequence.

34. A single exon nucleic acid probe as claimed in any one of claims 13 20, wherein said probe lacks homopolymeric stretches of A or T.

293

35. A method of measuring gene expression in a sample derived from human heart, comprising: contacting the microarray of claim 12, with a first collection of detectably labeled nucleic acids, said first collection of nucleic acids derived from mRNA of human heart; and then measuring the label detectably bound to each probe of said microarray.

36. A method of identifying exons in a eukaryotic genome, comprising:

algorithmically predicting at least one exon from genomic sequence of said eukaryote; and then detecting specific hybridization of detectably labeled nucleic acids to a single exon probe, wherein said detectably labeled nucleic acids are derived from mRNA from the heart of said eukaryote, said probe is single exon probe having a fragment identical in sequence to, or complementary in sequence to, said predicted exon, said probe is included within a microarray according to claim 12, and said fragment is selectively hybridizable at high stringency.

37. A method of assigning exons to a single gene, 25 comprising:

1 identifying a plurality of exons from genomic sequence according to the method of claim 23; and then measuring the expression of each of said exons in a plurality of tissues and/or cell types using hybridization to single exon microarrays having a probe with said exon, wherein a common pattern of expression of said exons in said plurality of tissues and/or cell types indicates that 35 the exons should be assigned to a single gene.

294

38. A nucleic acid sequence as set out in any of SEQ ID NOs: 1 10,283 which encodes a peptide.

39. A peptide encoded by a sequence as set out in any of SEQ ID Nos: 1 10,283.

40. A peptide comprising a sequence as set out in any of SEQ ID Nos: 10,284 - 15,322.