GB2359357A - FRET-based assay to detect RNA binding antimicrobials - Google Patents

FRET-based assay to detect RNA binding antimicrobials Download PDF

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Publication number
GB2359357A
GB2359357A GB0030862A GB0030862A GB2359357A GB 2359357 A GB2359357 A GB 2359357A GB 0030862 A GB0030862 A GB 0030862A GB 0030862 A GB0030862 A GB 0030862A GB 2359357 A GB2359357 A GB 2359357A
Authority
GB
United Kingdom
Prior art keywords
rna
antimicrobial
target rna
binding
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0030862A
Other languages
English (en)
Other versions
GB0030862D0 (en
GB2359357A8 (en
Inventor
David Justin Charles Knowles
Jonathan Karn
Alastair Iain Hamilton Murchie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vernalis Cambridge Ltd
Original Assignee
Ribotargets Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/465,355 external-priority patent/US6316194B1/en
Priority claimed from GBGB9929810.1A external-priority patent/GB9929810D0/en
Application filed by Ribotargets Ltd filed Critical Ribotargets Ltd
Publication of GB0030862D0 publication Critical patent/GB0030862D0/en
Publication of GB2359357A publication Critical patent/GB2359357A/en
Publication of GB2359357A8 publication Critical patent/GB2359357A8/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB0030862A 1999-12-16 2000-12-18 FRET-based assay to detect RNA binding antimicrobials Withdrawn GB2359357A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/465,355 US6316194B1 (en) 1999-12-16 1999-12-16 Methods and kits for discovery of RNA-binding antimicrobials
GBGB9929810.1A GB9929810D0 (en) 1999-12-16 1999-12-16 Methods and kits for discovery of RNA-binding antimicrobials

Publications (3)

Publication Number Publication Date
GB0030862D0 GB0030862D0 (en) 2001-01-31
GB2359357A true GB2359357A (en) 2001-08-22
GB2359357A8 GB2359357A8 (en) 2002-03-04

Family

ID=26316128

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0030862A Withdrawn GB2359357A (en) 1999-12-16 2000-12-18 FRET-based assay to detect RNA binding antimicrobials

Country Status (4)

Country Link
EP (1) EP1238109A2 (de)
AU (1) AU2200401A (de)
GB (1) GB2359357A (de)
WO (1) WO2001044505A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0020083D0 (en) * 2000-08-15 2000-10-04 Ribo Targets Ltd Assay 14
WO2009115287A1 (de) * 2008-03-17 2009-09-24 Technische Universität Dortmund Thiostrepton-derivate

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593835A (en) * 1995-05-12 1997-01-14 President And Fellows Of Harvard College Methods and kits for RNA binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
WO1998039484A1 (en) * 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
WO1999013338A1 (en) * 1997-09-12 1999-03-18 Genelabs Technologies, Inc. dsRNA/dsRNA-BINDING PROTEIN METHODS AND COMPOSITIONS
WO1999064625A2 (en) * 1998-06-05 1999-12-16 Ribotargets Limited Methods and kits for discovery of rna-binding compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712096A (en) * 1994-08-23 1998-01-27 University Of Massachusetts Medical Center Oligoribonucleotide assays for novel antibiotics

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593835A (en) * 1995-05-12 1997-01-14 President And Fellows Of Harvard College Methods and kits for RNA binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
WO1998039484A1 (en) * 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
WO1999013338A1 (en) * 1997-09-12 1999-03-18 Genelabs Technologies, Inc. dsRNA/dsRNA-BINDING PROTEIN METHODS AND COMPOSITIONS
WO1999064625A2 (en) * 1998-06-05 1999-12-16 Ribotargets Limited Methods and kits for discovery of rna-binding compounds

Also Published As

Publication number Publication date
WO2001044505A2 (en) 2001-06-21
WO2001044505A3 (en) 2001-12-20
EP1238109A2 (de) 2002-09-11
GB0030862D0 (en) 2001-01-31
AU2200401A (en) 2001-06-25
GB2359357A8 (en) 2002-03-04

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Legal Events

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)