WO2001044505A3 - Methods and kits for discovery of rna-binding antimicrobials - Google Patents

Methods and kits for discovery of rna-binding antimicrobials Download PDF

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Publication number
WO2001044505A3
WO2001044505A3 PCT/GB2000/004844 GB0004844W WO0144505A3 WO 2001044505 A3 WO2001044505 A3 WO 2001044505A3 GB 0004844 W GB0004844 W GB 0004844W WO 0144505 A3 WO0144505 A3 WO 0144505A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding
test compound
target rna
group
rna
Prior art date
Application number
PCT/GB2000/004844
Other languages
French (fr)
Other versions
WO2001044505A2 (en
Inventor
David Justin Charles Knowles
Jonathan Karn
Alastair Iain Hamilton Murchie
Georg Friedrich Lentzen
Original Assignee
Ribotargets Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/465,355 external-priority patent/US6316194B1/en
Priority claimed from GBGB9929810.1A external-priority patent/GB9929810D0/en
Application filed by Ribotargets Ltd filed Critical Ribotargets Ltd
Priority to EP00985595A priority Critical patent/EP1238109A2/en
Priority to AU22004/01A priority patent/AU2200401A/en
Publication of WO2001044505A2 publication Critical patent/WO2001044505A2/en
Publication of WO2001044505A3 publication Critical patent/WO2001044505A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides a method for determining whether a test compound binds to a target RNA, the method comprising the steps of: a) contacting the test compound with a pair of indicator molecules comprising an antimicrobial labelled with a donor group or an acceptor group and the target RNA labelled with a complementary acceptor or donor group, the pair being capable of binding to each other in an orientation that permits the donor group to come into sufficient proximity to the acceptor group to permit fluorescent resonance energy transfer and/or quenching to take place; and b) measuring the fluorescence of the target RNA and/or the antimicrobial in the presence of the test compound and comparing this value to the fluorescence of a standard.
PCT/GB2000/004844 1999-12-16 2000-12-15 Methods and kits for discovery of rna-binding antimicrobials WO2001044505A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00985595A EP1238109A2 (en) 1999-12-16 2000-12-15 Methods and kits for discovery of rna-binding antimicrobials
AU22004/01A AU2200401A (en) 1999-12-16 2000-12-15 Methods and kits for discovery of rna-binding antimicrobials

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/465,355 US6316194B1 (en) 1999-12-16 1999-12-16 Methods and kits for discovery of RNA-binding antimicrobials
GB9929810.1 1999-12-16
US09/465,355 1999-12-16
GBGB9929810.1A GB9929810D0 (en) 1999-12-16 1999-12-16 Methods and kits for discovery of RNA-binding antimicrobials

Publications (2)

Publication Number Publication Date
WO2001044505A2 WO2001044505A2 (en) 2001-06-21
WO2001044505A3 true WO2001044505A3 (en) 2001-12-20

Family

ID=26316128

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004844 WO2001044505A2 (en) 1999-12-16 2000-12-15 Methods and kits for discovery of rna-binding antimicrobials

Country Status (4)

Country Link
EP (1) EP1238109A2 (en)
AU (1) AU2200401A (en)
GB (1) GB2359357A (en)
WO (1) WO2001044505A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0020083D0 (en) * 2000-08-15 2000-10-04 Ribo Targets Ltd Assay 14
WO2009115287A1 (en) * 2008-03-17 2009-09-24 Technische Universität Dortmund Thiostrepton derivatives

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593835A (en) * 1995-05-12 1997-01-14 President And Fellows Of Harvard College Methods and kits for RNA binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
US5712096A (en) * 1994-08-23 1998-01-27 University Of Massachusetts Medical Center Oligoribonucleotide assays for novel antibiotics
WO1999013338A1 (en) * 1997-09-12 1999-03-18 Genelabs Technologies, Inc. dsRNA/dsRNA-BINDING PROTEIN METHODS AND COMPOSITIONS
WO1999064625A2 (en) * 1998-06-05 1999-12-16 Ribotargets Limited Methods and kits for discovery of rna-binding compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998039484A1 (en) * 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712096A (en) * 1994-08-23 1998-01-27 University Of Massachusetts Medical Center Oligoribonucleotide assays for novel antibiotics
US5593835A (en) * 1995-05-12 1997-01-14 President And Fellows Of Harvard College Methods and kits for RNA binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
WO1999013338A1 (en) * 1997-09-12 1999-03-18 Genelabs Technologies, Inc. dsRNA/dsRNA-BINDING PROTEIN METHODS AND COMPOSITIONS
WO1999064625A2 (en) * 1998-06-05 1999-12-16 Ribotargets Limited Methods and kits for discovery of rna-binding compounds

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CARDULLO R A ET AL: "DETECTION OF NUCLEIC ACID HYBRIDIZATION BY NONRADIATIVE FLUORESCENCE RESONANCE ENERGY TRANSFER", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 85, no. 23, 1 December 1988 (1988-12-01), pages 8790 - 8794, XP000453537, ISSN: 0027-8424 *
WANG Y ET AL: "Specificity of aminoglycoside binding to RNA constructs derived from the 16S rRNA decoding region and the HIV-RRE activator region", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 36, 1997, pages 768 - 779, XP002120808, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2001044505A2 (en) 2001-06-21
AU2200401A (en) 2001-06-25
EP1238109A2 (en) 2002-09-11
GB2359357A8 (en) 2002-03-04
GB0030862D0 (en) 2001-01-31
GB2359357A (en) 2001-08-22

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