GB2295394A - Vaccine Compositions - Google Patents

Vaccine Compositions

Info

Publication number
GB2295394A
GB2295394A GB9601739A GB9601739A GB2295394A GB 2295394 A GB2295394 A GB 2295394A GB 9601739 A GB9601739 A GB 9601739A GB 9601739 A GB9601739 A GB 9601739A GB 2295394 A GB2295394 A GB 2295394A
Authority
GB
United Kingdom
Prior art keywords
fusion proteins
protein
vaccine compositions
tetc
het
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9601739A
Other versions
GB9601739D0 (en
GB2295394B (en
Inventor
Mohammed Anjam Khan
Steven Neville Chatfield
Gordon Dougan
Carlos Estenio Hormaeche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medeva Holdings BV
Original Assignee
Medeva Holdings BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9401787A external-priority patent/GB9401787D0/en
Application filed by Medeva Holdings BV filed Critical Medeva Holdings BV
Priority to GB9601739A priority Critical patent/GB2295394B/en
Priority claimed from PCT/GB1994/001647 external-priority patent/WO1995004151A2/en
Publication of GB9601739D0 publication Critical patent/GB9601739D0/en
Publication of GB2295394A publication Critical patent/GB2295394A/en
Application granted granted Critical
Publication of GB2295394B publication Critical patent/GB2295394B/en
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention provides a DNA construct comprising a DNA sequence encoding a fusion protein of the formula: TetC-(Z)a-Het, wherein: TetC is the C fragment of tetanus toxin, or a protein comprising the epitopes thereof; Het is a heterologous protein, Z is an amino acid, and a is zero or a positive integer, provided that (Z)a does not include the sequence Gly-Pro. The invention also provides replicable expression vectors containing the constructs, bacteria transformed with the constructs, the fusion proteins per se and vaccine compositions formed from the fusion proteins or attenuated bacteria expressing the fusion proteins. <IMAGE>
GB9601739A 1994-01-31 1994-07-29 DNA encoding a fusion protein comprising the C fragment of tetanus toxin Revoked GB2295394B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9601739A GB2295394B (en) 1994-01-31 1994-07-29 DNA encoding a fusion protein comprising the C fragment of tetanus toxin

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9401787A GB9401787D0 (en) 1994-01-31 1994-01-31 Vaccine compositions
GB9601739A GB2295394B (en) 1994-01-31 1994-07-29 DNA encoding a fusion protein comprising the C fragment of tetanus toxin
PCT/GB1994/001647 WO1995004151A2 (en) 1993-07-30 1994-07-29 Vaccine compositions containing recombinant tetc-fusion proteins

Publications (3)

Publication Number Publication Date
GB9601739D0 GB9601739D0 (en) 1996-03-27
GB2295394A true GB2295394A (en) 1996-05-29
GB2295394B GB2295394B (en) 1997-09-24

Family

ID=26304238

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9601739A Revoked GB2295394B (en) 1994-01-31 1994-07-29 DNA encoding a fusion protein comprising the C fragment of tetanus toxin

Country Status (1)

Country Link
GB (1) GB2295394B (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989006974A2 (en) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. T-cell epitope as carriers molecule for conjugate vaccines
EP0432965A1 (en) * 1989-12-08 1991-06-19 Smithkline Beecham Corporation Malaria vaccine
WO1991009621A1 (en) * 1989-12-29 1991-07-11 Institut Pasteur PEPTIDE FRAGMENT COMPRISING A SEQUENCE FROM THE 28kDa PROTEIN OF SCHISTOSOMA MANSONI, AND VACCINATING AND/OR THERAPEUTIC COMPOSITIONS COMPRISING SAID FRAGMENT
WO1992015689A1 (en) * 1991-03-05 1992-09-17 The Wellcome Foundation Limited Expression of recombinant proteins in attenuated bacteria
WO1993008290A1 (en) * 1991-10-16 1993-04-29 University Of Saskatchewan Enhanced immunogenicity using leukotoxin chimeras
WO1994003615A1 (en) * 1992-07-31 1994-02-17 Medeva Holdings B.V. Expression of recombinant fusion proteins in attenuated bacteria

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989006974A2 (en) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. T-cell epitope as carriers molecule for conjugate vaccines
EP0432965A1 (en) * 1989-12-08 1991-06-19 Smithkline Beecham Corporation Malaria vaccine
WO1991009621A1 (en) * 1989-12-29 1991-07-11 Institut Pasteur PEPTIDE FRAGMENT COMPRISING A SEQUENCE FROM THE 28kDa PROTEIN OF SCHISTOSOMA MANSONI, AND VACCINATING AND/OR THERAPEUTIC COMPOSITIONS COMPRISING SAID FRAGMENT
WO1992015689A1 (en) * 1991-03-05 1992-09-17 The Wellcome Foundation Limited Expression of recombinant proteins in attenuated bacteria
WO1993008290A1 (en) * 1991-10-16 1993-04-29 University Of Saskatchewan Enhanced immunogenicity using leukotoxin chimeras
WO1994003615A1 (en) * 1992-07-31 1994-02-17 Medeva Holdings B.V. Expression of recombinant fusion proteins in attenuated bacteria

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIO/TECHNOLOGY, Vol. 10, No. 8, 1992, pages 888-892 *
FEMS Symposium, No. 73, "Bacterial Protein Toxins", published 1994, Freer, J. et al (Ed.) *
J. IMMUNOLOGY, Vol. 141, No. 5, 1988, pages 1687-1694 *
NUCLEIC ACIDS RESEARCH, Vol. 19, No. 11, 1991, pages 2889-2892 *

Also Published As

Publication number Publication date
GB9601739D0 (en) 1996-03-27
GB2295394B (en) 1997-09-24

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Legal Events

Date Code Title Description
773K Patent revoked under sect. 73(2)/1977

Free format text: PATENT REVOKED ON 20030915