GB2286340A - Intracerebral injection apparatus for non-anaesthetized rodents - Google Patents
Intracerebral injection apparatus for non-anaesthetized rodents Download PDFInfo
- Publication number
- GB2286340A GB2286340A GB9501291A GB9501291A GB2286340A GB 2286340 A GB2286340 A GB 2286340A GB 9501291 A GB9501291 A GB 9501291A GB 9501291 A GB9501291 A GB 9501291A GB 2286340 A GB2286340 A GB 2286340A
- Authority
- GB
- United Kingdom
- Prior art keywords
- head
- rodents
- workplate
- animal
- baseplate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000002347 injection Methods 0.000 title claims abstract description 16
- 239000007924 injection Substances 0.000 title claims abstract description 16
- 241000283984 Rodentia Species 0.000 title claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims description 12
- 239000000837 restrainer Substances 0.000 claims description 2
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 206010002091 Anaesthesia Diseases 0.000 description 3
- 238000001949 anaesthesia Methods 0.000 description 3
- 230000037005 anaesthesia Effects 0.000 description 3
- 230000003444 anaesthetic effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 206010067482 No adverse event Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229940124326 anaesthetic agent Drugs 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Landscapes
- Veterinary Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Housing For Livestock And Birds (AREA)
- Catching Or Destruction (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
Abstract
Apparatus for immobilizing a rodent enabling intracerebral injection comprises a head rest 2 and a longitudinally displaceable positioning block 5 with a V-shaped recess 6 that engages the anterior of the rodents head. A workplate 12 can slide down guide rods 13 as far as stop sleeves 14 and has at least one funneled guide hole 15 through which an injection syringe 18 can be directed. A hoop 11 extends over the recess 6 and grips the nose of the rodent while guide rods 17 enclose the posterior of the rodents head. Accurate and rapid intracerebral injections can thus be easily performed without anaesthetizing the rodent. <IMAGE>
Description
An Apparatus for Intracerebral
Iniections in Non-Anesthetized Rodents
This invention relates to an apparatus for intracerebral injections in non-anaesthetized rodents in neuropharmacology.
Hitherto animals to be injected, e.g. for experimental purposes, have been held by hand during the injection. In this case it has been necessary to keep the animal quiet by anaesthesia in order to allow the injection to be performed accurately on target. Great skill was need by the person performing the injection to ensure accurate administration, and the manipulation times were relatively long. Furthermore, the anaesthetic administered to the rodents influenced the results of the experiments.
It is an object of the present invention to develop an apparatus with which intracerebral injections can be performed in rapid sequence with maximum accuracy in rodents without anaesthesia.
We have found that this object is achieved by an apparatus which includes a head restrainer for the animal to be treated comprising a baseplate with a rest for the head of the animal and a positioning unit, which can be displaced and fixed relative to the baseplate, for the anterior part of the head, and a workplate which can be moved towards the positioning unit and has at least one tubular guide tube for an injection syringe which is arranged in use to be directed towards the head region.
An embodiment of the invention will now be described, by way of example only, with particular reference to the accompanying drawing the sole figure of which diagrammatically depicts an exemplary embodiment of apparatus for intracerebral injection of rodents.
The apparatus comprises an elongate baseplate 1 having a projection 3 at one end which forms a rest 2 for the head of the animal and slopes down towards the front and above which a positioning unit 4 for the anterior part of the head can be displaced and fixed. The positioning unit consists of a block 5 which is provided with a V-shaped recess 6 enclosing the anterior part of the head, and is guided lengthwise in elongate apertures 8 in holding plates 7 which are fixed on the sides of the baseplate 1. The block 5 is fixed in position by means of screws 9 with knurled heads 10. A holding-down hoop 11 extends over the recess 6 to grip over the nose region of the head which is immobilized in block 5.
A workplate 12 is arranged above the baseplate 1 and can be moved relative to the baseplate 1, guided by sliding rods 13 anchored in the baseplate, as far as stop sleeves 14 on the positioning unit 4. Above the positioning unit, the workplate 12 is equipped with at least one tubular guide 15 which penetrates obliquely through the workplate and through which a disposable tube can be passed to perform a targeted trephination on the head of the animal. The intracerebral injection then takes places through the tubular guide. It is advantageous to provide the guide 15 with an introduction funnel 16 or the like conical introductory inlet.
It is furthermore expedient to attach guide rods 17 laterally on the workplate 12 above the rest 2 for the head of the animal, which enclose the posterior region of the head of the animal when the workplate is lowered.
The use of the apparatus according to the invention as a stereotactic appliance for intracerebral injections on rodents shows the following advantages:
1. Possibilities of use in screening methods
- rapid performance
- different head sizes can be treated, and
thus the animal weight range is extended.
2. No anaesthesia necessary
- no adverse effects on the results of
experiments
- saving of anaesthetics and technical
equipment including environmental
protection measures
- more safety for operators
- no adverse environmental effects.
3. Good reproducibility of the results of
experiments.
Claims (6)
1. An apparatus fo- intracerebral injections in non-anaesthetized rodents, including a head restrainer for the animal to be treated comprising a baseplate with a rest for the head of the animal and a positioning unit, which can be displaced and fixed relative to the baseplate, for the anterior part of the head, and a workplate which can be moved towards the positioning unit and has at least one tubular guide for an injection syringe which is arranged in use to be directed towards the head region.
2. An apparatus according to claim 1, wherein the positioning unit consists of a block having a V-shaped recess and which is guided between lateral holding plates each provided with an elongate aperture, a holding-down hoop being arranged above the recess.
3. An apparatus according to claim 1 or 2, wherein the workplate can travel on slide rods anchored in the baseplate.
4. An apparatus according to any of the preceding claims, wherein the workplate is equipped with two guide rods for enclosing the posterior region of the head of the animal.
5. An apparatus according to any of the preceding claims, wherein the tubular guide has a tapered introduction inlet.
6. An apparatus for intracerebral injections in non-anaesthetized rodents, the apparatus being constructed and arranged substantially as herein described with reference to, and as illustrated in, the accompanying drawing.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE9401191U DE9401191U1 (en) | 1994-01-25 | 1994-01-25 | Device for intracerebral injections in non-anesthetized rodents |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB9501291D0 GB9501291D0 (en) | 1995-03-15 |
| GB2286340A true GB2286340A (en) | 1995-08-16 |
Family
ID=6903658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB9501291A Withdrawn GB2286340A (en) | 1994-01-25 | 1995-01-24 | Intracerebral injection apparatus for non-anaesthetized rodents |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE9401191U1 (en) |
| GB (1) | GB2286340A (en) |
| IE (1) | IE950056A1 (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4254767A (en) * | 1979-07-30 | 1981-03-10 | Mixon Jr James L | Laboratory device for small animals |
-
1994
- 1994-01-25 DE DE9401191U patent/DE9401191U1/en not_active Expired - Lifetime
-
1995
- 1995-01-24 GB GB9501291A patent/GB2286340A/en not_active Withdrawn
- 1995-01-25 IE IE950056A patent/IE950056A1/en not_active IP Right Cessation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4254767A (en) * | 1979-07-30 | 1981-03-10 | Mixon Jr James L | Laboratory device for small animals |
Also Published As
| Publication number | Publication date |
|---|---|
| GB9501291D0 (en) | 1995-03-15 |
| DE9401191U1 (en) | 1994-03-24 |
| IE950056A1 (en) | 1995-07-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |