GB2261877A - Animal feed additive comprising enzyme and amino acid - Google Patents

Animal feed additive comprising enzyme and amino acid Download PDF

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GB2261877A
GB2261877A GB9224171A GB9224171A GB2261877A GB 2261877 A GB2261877 A GB 2261877A GB 9224171 A GB9224171 A GB 9224171A GB 9224171 A GB9224171 A GB 9224171A GB 2261877 A GB2261877 A GB 2261877A
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enzyme
amino acid
improving
feed additive
activity
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GB9224171D0 (en
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Shunitsu Konno
Ichiro Matsuura
Kunikatsu Shirahata
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KH Neochem Co Ltd
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Kyowa Hakko Kogyo Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01011—Dextranase (3.2.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

A concentrated animal feed additive containing a plant tissue-destruction enzyme and at least one essential amino acid effective for increasing milk yield, improving milk qualities, promoting growth, improving the meat qualities and elevating the breeding efficiency of animals. The enzyme may comprise at least one of cellulase, xylanase, mannanase, laminarinase, pectinase, amylase, ligninase, protease and dextranase, and the amino acid selected from lysine, methionine, threonine and tryptophan.

Description

_) -. - 1 _ C. 2 1 --- ANIMAL FEED ADDITIVE FfELD OF THE INVENTION This
invention relates to an animal f eed additive which is effective for increasing milk yield, improving milk qualities, promoting growth, improving meat qualities or elevating the breeding efficiency of animals, in particular, those having a ruminant stomach (hereinafter called ruminants).
BACKGROUND OF THE INVENTION
It has been known to add enzyme compositions containing enzymes having plant tissue-destruction activity such as cellulase to cattle feeds in order to increase the milk yield and to improve the milk qualities of milk cows (Biotechnology Letters, 9 (5), 369 (1987); and U.S.Patent 4,144, 354].
It has previously been considered that it is sufficient to supply ruininants with a feed mainly comprising roughage without providing essential amino acids since microorganisms produce essential amino acids in the rumen of ruminants. With the spread of the feeding of milk cows capable of providing an increased milk yield in recent years, however, it seems that the essential amino acids produced by microorganisms in vivo cannot provide a sufficient amount of protein. It is, therefore, necessary to supply a definite amount of non-digestible proteins - 1 which would pass through the rumen. For example, attempts have been made to add protected amino acids to feeds [i. Dairy Sci., 69, 2348 (1986)]. However, none of these attempts have been successfully put into practical use, since the effects thus achieved are unsatisfactory and, furthermore, the protected amino acids are expensive. It is also reported to orally administer a liquid composition containing amino acids to ruminants (Japanese Published Unexamined Patent Application No. 255047/90). However this method suffers from problems including decomposition of the amino acids contained in the liquid composition as well as difficulty in administration and feeding.
The need exists for an inexpensive animal feed additive of good quality which is effective for increasing milk yield, improving milk qualities, promoting growth, improving meat qualities or elevating the breeding efficiency of ruminants.
SUMMARY OF THE INVENTION
The present inventors have found that oral administration of a feed with an animal feed additive which contains an enzyme having plant tissuedestruction activity together with one or more essential amino acids, is highly effective for increasing milk yield, improving milk qualities, promoting growth, improving meat qualities or elevating the breeding efficiency of the animals.
Z An object of the present invention is to provide a concentrated animal feed additive which consists essentially of an enzyme having plant tissuedestruction activity and at least one essential amino acid.
DETAILED DESCRIPTION OF THE INVENTION
Any enzyme having plant tissue-destruction activity, such as fibrinolytic activity, CMCase activity, saccharific activity, proteolytic activity and the like, can be used in the present invention, so long as the effects of the present invention can be achieved thereby.
Examples of suitable enzymes include cellulase, hemicellulases such as xylanase and mannanase, laminarinase, pectinase, amylase, ligninase, protease and dextranase. These enzymes or mixtures of them may be used alone or in combinations of two or more. A preferred example of an enzymecontaining mixture having plant tissue-destruction activity is obtained by incubating a microorganism of Basidiomycetes, belonging to the genus Fomitopsis, Irpex or Ascomyeetes belonging to the genus Trichoderma.
A specific example thereof include Driselasel (manufactured by Kyowa Hakko Kogyo Co., Ltd.) which is an enzyme composition obtained by culturing Basidiomycetes belonging to the species Irpex lacteus having plant tissuedestruction activity, in particular, intense plant cell wall-destruction activity such as activities of cellulase, laminarinase, xylanase, pectinase, amylase, protease and dextranase (U.S.Patent 4,144, 354).
Examples of the essential amino acids to be used in the present invention include valine, leucine, isoleucine, threonine, phenylalanine, tryptophan, methionine, lysine, histidine, arginine and salts thereof. Lysine, methionine, threonine and tryptophan are particularly suitable.
Any essential amino acid obtainable by synthesis or fermentation may be used. Further, for example, protected amino acids coated with an organic polymer or protected amino acid derivatives may be employed. Preferred protected amino acids are those which can liberate essential amino acids in the rumen or can be converted into essential amino acids.
The animal feed additive of the present invention may be given to an animal at a dose of from 0.01 to 0.5 g of amino acid, per kg body weight of the animal per day, which corresponds to a fiber-destructive potency of from 0.8 to 80 in terms of enzymes, per kg body weight of the animal per day. The unit of an enzyme having plant tissuedestruction activity used herein means "fiber-destructive potency" determined in accordance with the "fiberdestructive potency test" [Extra Edition No. 28 of Official Gazette, pages 8 - 9, March 20, 1990, published by Printing Bureau of the Japanese Ministry of Finance].
The fiber-destructive potency test can be carried out as follows.
A sample is accurately weighed and dissolved in 1 M acetic acid/sodium acetate buffer having a pH optimal for the enzyme contained in the sample. A 5 ml portion of the sample solution is charged into an L tube and allowed to stand for 5 minutes at 37 t 5 OC. Immediately after two pieces of filter paper (1 cm x I cm) are added thereto, the tube is shaken at 65 rpm, at an amplitude of 60 mm and at 37 0.5 OC. The period of time required for completely destructing filter paper into fine fibers is measured and the fiber-destructive potency unit is calculated in accordance with the following equation.
Fiber-destructive 1 potency unit X 1,000 per gram T x W average time (min) required for completely destructing filter paper (except for the longest and the shortest) amount of the sample (g) in 5 ml of the sample solution Examples of the dose of each component in the feed additives per animal per day are given below.
(1) Milk cow (average body weight: 500 - 600 kg) enzyme 400 - 4,000 (fiber-destructive potency unit) L-lysine hydrochloride 5 - 50 (g) DL-methionine 1 - 10 (g] L-threonine 0.2 - 5 (g) L-tryptophan 0.1 - 3 (g) (2) Cattle on rearing (calf) (average body weight: 100 - 2 00 kg) enzyme 80 - 800 (fiber-destructive potency unit) L-lysine hydrochloride 1 DL-methionine 0.2 - 5 L-threonine 0.05 - 3 (3) _ g) 9) 9) L-tryptophan 0.02 - 2 (g) Fattening cattle (average body weight: 500 - 600 kg) enzyme 400 - 4,000 (fiber-destructive potency unit) 5 5 0 (g) 1 10 (g) L-threonine 0.2 5 (g) L-tryptophan 0.1 3 (g) By animals we mean livestock and domestic animals such as cattle, goats, sheep, camels, deer and reindeer.
The feed additives according to the present invention may be formulated into, for example, a powder, pellets or an aqueous solution and given to the animals either alone or in the form of a mixture with other feeds.
L-lysine hydrochloride DL-methionine - 6 According to the present invention, an animal feed additives, which are excellent in improving milk yield, improving milk qualities, promoting growth, improving meat qualities or elevating the breeding efficiency of animals when orally administered, can be obtained by adding an enzyme having plant tissuedestruction activity and one or more essential amino acids to a traditional animal feed.
To further illustrate the present invention in greater detail, the following examples are given, which are not to be construed to limit the scope of the present invention.
EXAMPLE 1 Improvement in milk yield and milk qualities of milk cows female lactating adult Holstein milk cows were divided into six groups (A, B, C, D, E and F) of 5 cows each. In a preliminary test, all 30 cows were fed with a traditional feed of the composition shown in Table 1.for 10 days (the preliminary test period). Next, each of six different pre- mix feeds containing the additives according to the present invention listed in Table 2 was mixed with the traditional feed of Table 1 in such a manner as to give a dose of 100 g of pre-mix per animal per day. Then the cows were fed with the feed thus obtained for 30 days (the 7 - main test period). The milk yield was measured and, milk f at percentage, non-f at solid percentage of the milk and milk protein percentage were determined with Milko-scan 133 (AS. N. Foss. Electric) and the results reported in Table 3.
TABLE 1 Composition of traditional feed Corn Barley with husk Heated soybean Cotton seed meal Beet pulp Soybean meal Corn gluten feed Lucerne Hay cube Sudan grass Oat (kq/animal/day) 4.3 3.6 1.5 1.5 3.3 1.5 1.0 4.0 1.3 2.6 1.3 TABLE 2
Composition of pre-mix feeds (% by-weiqht) A B c D E F L-lysine hydrochloride 10 10 10 10 0 0 DL-methionine 2 2 2 2 0 0 L-threonine 0.5 0.5 0 0 0 0 L-tryptophan 0.5 0.5 0 0 0 0 Driselase 1 0 1 0 1 0 Glutamic acid fermenta- 34 34 34 34 34 34 tion meal Citric acid 2 2 2 2 2 2 vitamin/mineral premix 10 10 10 10 10 10 Algae powder 10 10 10 10 10 10 Lucerne meal 20 20 20 20 20 20 Rice bran 10 11 11 12 23 24 Total 100 100 100 100 100 100 Enzyme activity in Driselase = 800 fiber-destructive potency unit/g.
9 - TABLE 3
Item Non-f at Milk Milk yield Milk fat solid protein (kq/daV) percentaqe percentage percentaqe _ Test p 1 M.Z p m p X p X Group A 27.0 29.9 3.4 3.8 8.3 9.4 3.0 3.5 Group B 27.3 28.6 3.4 3.5 8.3 8.6 3.0 3.2 Group C 27.5 30.0 3.4 3.7 8.3 9.2 3.0 3.3 Group D 27.3 28.5 3.4 3.5 8.3 8.5 3.0 3.1 Group E 27.2 28.0 3.4 3.5 8.3 8.5 3.0 3.1 Group F 27.0 27.1 3.4 3.4 8.3 8.3 3.0 3.0 1: P means the preliminary test period. 2: X means the main test period.
(Expressed in the average in each test period.) Table 4 shows the percent gain in each item during the main test period based on the average in the preliminary test period (prior to incorporating the premix).
TABLE 4
Group A Group B Group C Group D Group E Group F Milk yieli Percentace 10.7 4.8 9.1 4.4 2.9 0.4 Non-f at Milk Milk fat solid protein Percentaqe Percentaqe percentaqe 11.8 13.3 16.7 2.9 8.8 2.9 2.9 0.0 3.6 10.8 2.4 2.4 0.0 6.7 10.0 3.3 3.3 0.0 These results indicate that the administration of the feed additives of the present invention (groups A and C) to milk cows resulted in an increase in milk yield and improvement in milk qualities.
EXAMPLE 2
Growth promotion of calves female Holstein calves aged 5 to 6 months were divided into six groups (A, B, C, D, E and F) of 5 calves each. In a preliminary test, all calves were fed with a traditional feed comprising 5 kg/day of a roughage (dry pasturage) and 4 kg/day of a concentrated feed for grower, which is referred to as the "traditional feed A" hereinafter, for 10 days (the preliminary test period). Next, each of six different pre-mix feeds containing the additives according to the present invention listed in the above Table 2 was mixed with the traditional feed A in such a manner as to give a dose of 50 g of pre-mix per animal per day. Then the- calves were f ed with the feed thus obtained for 90 ays (the main test period). The body weight gain during each test period was measured.
The body weight gain ratios based on the control group (group F) were determined according to the following equation.
Body weight gain ratio Body weight gain of each group Body weight gain of control group Table 5 shows the results.
X 100 - 100 TABLE 5
Body %yeight Body weight at the end at the end Body weight of pre. test of main test aain ratio (kglanimal) (kg/animal) M Group A 165 290 21.4 Group B 165 277 8.7 Group C 164 279 11.7 Group D 170 280 6.8 Group E 163 271 4.9 Group F 170 273 0.0 These results indicate that the growth of the calves was promoted by feeding them the feed additives of the present invention (groups A and C).
EXAMPLE 3 Impcovement in meat texture of fatteninq cattle Three months bef ore shipping, 15 Holstein oxen aged 32 months were divided into three groups (G, H and I) of 5 oxen each. In a preliminary test, all oxen fed with a traditional feed comprising 2 kg/day of a roughage (rice straw) and 10 kg/day of a concentrated feed for finisher, which is referred to as the "traditional feed B11 hereinafter, for 10 days (the preliminary test period). Next, each of three different pre-mix feeds containing the additives according to the present invention listed in the following Table 6 was mixed with the traditional feed B in such a manner as to give a dose of 100 g of pre-mix per animal per day. The oxen were f ed with the f eed thus obtained for 90 days (the main test period). The body weight during each test period was measured. Further the rib eye area (cm2) at the slaughter/ shipping were measured by dissecting light split carcass between the 6th rib and the 7th rib and measuring the area of musculus longissimus thoracis (rib eye) of the section.
The body weight gain ratios based on the control group (group I) were determined according to the following equation.
Body weight gain ratio (%) = Body weight gain of each group Body weight gain of control group Table 7 shows the results.
- 14 X 100 - 100 i TABLE 6
Composition of pre-mix feeds (% by weight) G L-lysine hydrochloride DL-methionine L-threonine L-tryptophan Driselase Glutamic acid fermentation meal Citric acid Vitamin/mineral premix 10 Algae powder Lucerne meal Rice bran Total 2 1 34 2 H 0 0 0.5 0 0.5 0 1 34 2 10 20 23 I 0 0 0 0 0 34 2 10 20 24 100 100 Enzyme activity in Driselase = 800 fiber-destructive potency unit/g.
TABLE 7
Body weight_ Body weight at the end at the end Body weight Rib eye of pre. test of main test gain ratio area (kq/animal) (kq/animal) (%) _ (CM2 /animal) Group G 616 736 18.8 45 Group H 612 721 7.9 42 Group 1 620 721 0.0 40 These results indicate that the administration of the feed additives of the present invention (group G) increased the meat weight and enlarged the rib eye area of oxen before shipping. EXAMPLE 4 Feed for milk cow A feed for milk cow of the composition as listed in the following Table 8 was prepared and given to 5 female adult Holstein milk cows in an amount of 26,000 g/animal/day for 4 months. As a control, the same composition as in Table 8 except that L-lysine hydrochloride, DL-methionine, L- threonine and Driselase were replaced with the same amount of rice bran was prepared and given to cows of a control group.
At the initiation and completion of feed, the milk yield was measured and milk fat percentage, non-fat solid percentage and milk protein percentage were determined with Milko-scan 133 (AS. N. Foss Electric). The results are shown in Table 9.
TABLE 8 Composition of milk cow feed Corn Barley with husk Heated soybean Cotton seed meal Beet pulp Soybean meal Corn gluten feed Lucerne Hay cube Sudan grass Oat L-lysine hydrochloride DL-methionine L-threonine Driselase Glutamic acid fermentation meal Citric acid Vitamin/mineral premix Algae powder Lucerne meal Rice bran Total (q) 4300 3600 1500 1500 3300 1500 1000 4000 1300 2600 1300 2 0.5 1 34.5 2 26,000 Enzyme activity in Driselase 800 fiber-destructive potency unit/g - 17 TABLE 9
Non-f at Milk yield Milk fat solid Milk protein (kcr/day) Percentaqe Percentaqe percentaqe Invention - initiation of feed 27.5 3.4 8.3 3.0 completion of feed 28.9 3.7 8.8 3.3 Control initiation of feed 27.3 3.4 8.5 3.0 completion of feed 27.4 3.4 8.5 3.0 The results in Table 9 indicates that the administration of the feed additives of the present invention (Table 8) increased milk yield and improvement in milk qualities.
While the invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope thereof.

Claims (11)

1. A concentrated animal feed additive which consists essentially of an enzyme having plant tissuedestruction activity and at least one essential amino acid.
2. The concentrated animal feed additive as claimed in Claim 1, wherein said enzyme having plant tissuedestruction activity is one or more substances selected from a group consisting of cellulase, xylanase, mannanase, laminarinase, pectinase, amylase, ligninase, protease, dextranase and mixtures of two or more thereof.
3. The concentrated animal feed additive as claimed in Claim 1, wherein said essential amino acid is selected from lysine, methionine, threonine and tryptophan.
4. The concentrated animal feed additive for ruminant animals as claimed in Claim 1.
5. A method for improving milk yield and milk quality, promoting growth, improving meat quality or improving the breeding efficiency of ruminants comprising orally administering to said ruminant an effective amount of a composition containing an enzyme having plant tissuedestruction activity in combination with at least one essential amino acid.
6. The method of Claim 5 in which the amount of amino acid or amino acids administered is from about 0.01 to about 0. 5 g per kg of animal body weight per day and the amount of enzyme administered is from about 0.8 to about 80 f iber destructive potency unit per kg animal body weight per day.
7. The method of Claim 5, wherein said enzyme having plant tissuedestruction activity is one or more substances selected from a group consisting of cellulase, xylanase, mannanase, laminarinase, pectinase, amylase, ligninase, protease, dextranase and mixtures of two or more thereof.
8. The method of Claim 5, wherein said essential amino acid is selected from lysine, methionine, threonine and tryptophan.
9. A method as claimed in claim 5, substantially as hereinbefore described in any one of Examples 1 to 4.
10. An animal feed additive as claimed in claim 1, substantially as hereinbefore described in any one of Eximples 1 to 4.
11. The combined use of an enzyme having plant tissuedestruction activity and at least one essential amino acid for improving milk yield, improving milk quality, improving meat quality and/or improving the breeding efficiency of ruminants.
GB9224171A 1991-11-21 1992-11-18 Animal feed additive comprising enzyme and amino acid Withdrawn GB2261877A (en)

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* Cited by examiner, † Cited by third party
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WO1995017103A1 (en) * 1993-12-23 1995-06-29 Nutreco Nederland B.V. The use of mannan-rich materials in feedstuffs
GB2287867A (en) * 1994-03-30 1995-10-04 Finnfeeds Int Ltd Use of an enzyme for assisting an animal to digest protien
WO1996005739A1 (en) * 1994-08-19 1996-02-29 Finnfeeds International Limited An enzyme feed additive and animal feed including it
WO1996023421A1 (en) * 1995-02-03 1996-08-08 The Minister Of Agriculture Fisheries & Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain & Northern Ireland Amino acid compositions
WO1997001967A1 (en) * 1995-07-05 1997-01-23 Her Majesty The Queen In Right Of Canada, Represented By The Department Of Agriculture And Agri-Food Canada Enzyme additives for ruminant feeds
GB2327345A (en) * 1997-07-18 1999-01-27 Finnfeeds Int Ltd Use of a carbohydrase for controlling bacterial infection
WO2001049128A1 (en) * 1999-12-31 2001-07-12 Dr. Smoler - Feed Additives And Technologies Ltd. Dietary supplement
US6451063B1 (en) * 1996-09-25 2002-09-17 Genencor International, Inc. Cellulase for use in industrial processes
WO2004013322A2 (en) * 2002-08-02 2004-02-12 Klenzyme, Ltd. Degrading lignocellulosic materials
WO2007075343A3 (en) * 2005-12-15 2007-10-04 Chemgen Corp Enzymes for reduced immunological stress
WO2010109257A1 (en) * 2009-03-23 2010-09-30 Carlo Ghisalberti Composition to treat protein intolerance
WO2014207435A1 (en) * 2013-06-25 2014-12-31 Ucl Business Plc Anti-microbial agents and uses therefor
EP3344063B1 (en) 2015-09-01 2022-11-02 DuPont Nutrition Biosciences ApS Methods of increasing fat soluble vitamin uptake in feed

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10231710A1 (en) * 2002-07-13 2004-01-22 Degussa Ag Process for the production of bulk goods containing at least two active substances

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1071923A (en) * 1976-03-15 1980-02-19 Canada Packers Limited Calf feed containing sorangium enzymes
SU743668A1 (en) * 1978-06-20 1980-06-30 Научно-Исследовательский И Проектно- Технологический Институт Животноводства Центрально-Черноземной Зоны Whole milk substitute for young agricultural animals
US4533537A (en) * 1982-08-12 1985-08-06 Metallgesellschaft Aktiengesellschaft Process of producing sulfuric acid
HUT39576A (en) * 1985-03-08 1986-10-29 Muanyagipari Kutato Intezet Process for producing antibacterial prophylactic fodder additive
JPS6456350A (en) * 1987-08-26 1989-03-03 Mazda Motor Production of ceramic molding

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1071923A (en) * 1976-03-15 1980-02-19 Canada Packers Limited Calf feed containing sorangium enzymes
SU743668A1 (en) * 1978-06-20 1980-06-30 Научно-Исследовательский И Проектно- Технологический Институт Животноводства Центрально-Черноземной Зоны Whole milk substitute for young agricultural animals
US4533537A (en) * 1982-08-12 1985-08-06 Metallgesellschaft Aktiengesellschaft Process of producing sulfuric acid
HUT39576A (en) * 1985-03-08 1986-10-29 Muanyagipari Kutato Intezet Process for producing antibacterial prophylactic fodder additive
JPS6456350A (en) * 1987-08-26 1989-03-03 Mazda Motor Production of ceramic molding

Cited By (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995017103A1 (en) * 1993-12-23 1995-06-29 Nutreco Nederland B.V. The use of mannan-rich materials in feedstuffs
AU683720B2 (en) * 1994-03-30 1997-11-20 Finnfeeds International Limited Use of an enzyme for assisting an animal to digest protein
GB2287867A (en) * 1994-03-30 1995-10-04 Finnfeeds Int Ltd Use of an enzyme for assisting an animal to digest protien
EP0704167A1 (en) * 1994-08-19 1996-04-03 Finnfeeds International Ltd. An enzyme feed additive and animal feed including it
WO1996005739A1 (en) * 1994-08-19 1996-02-29 Finnfeeds International Limited An enzyme feed additive and animal feed including it
WO1996023421A1 (en) * 1995-02-03 1996-08-08 The Minister Of Agriculture Fisheries & Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain & Northern Ireland Amino acid compositions
WO1997001967A1 (en) * 1995-07-05 1997-01-23 Her Majesty The Queen In Right Of Canada, Represented By The Department Of Agriculture And Agri-Food Canada Enzyme additives for ruminant feeds
US5720971A (en) * 1995-07-05 1998-02-24 Her Majesty The Queen In Right Of Canada, As Represented By The Department Of Agriculture And Agri-Food Canada Enzyme additives for ruminant feeds
US6451063B1 (en) * 1996-09-25 2002-09-17 Genencor International, Inc. Cellulase for use in industrial processes
US7011964B2 (en) 1997-07-18 2006-03-14 Finnfeeds International Ltd. Use of an enzyme for the manufacture of an agent for controlling bacterial infection
GB2327345A (en) * 1997-07-18 1999-01-27 Finnfeeds Int Ltd Use of a carbohydrase for controlling bacterial infection
GB2327345B (en) * 1997-07-18 1999-06-23 Finnfeeds Int Ltd Use of an enzyme for manufacturing an agent for controlling bacterial infection
WO2001049128A1 (en) * 1999-12-31 2001-07-12 Dr. Smoler - Feed Additives And Technologies Ltd. Dietary supplement
WO2004013322A2 (en) * 2002-08-02 2004-02-12 Klenzyme, Ltd. Degrading lignocellulosic materials
WO2004013322A3 (en) * 2002-08-02 2004-07-29 Klenzyme Ltd Degrading lignocellulosic materials
US8778648B2 (en) 2005-12-15 2014-07-15 Eli Lilly And Company Enzymes for reduced immunological stress
US7914782B2 (en) 2005-12-15 2011-03-29 Chemgen Corporation Enzymes for reduced immunological stress
AU2006329927B2 (en) * 2005-12-15 2011-09-29 Elanco Us Inc. Enzymes for reduced immunological stress
RU2465005C2 (en) * 2005-12-15 2012-10-27 Эли Лилли Энд Компани Composition reducing immune stress (versions), method for improving growth indicators and method for reducing immune stress thereby
WO2007075343A3 (en) * 2005-12-15 2007-10-04 Chemgen Corp Enzymes for reduced immunological stress
HRP20080319B1 (en) * 2005-12-15 2015-11-20 Eli Lilly And Co. Enzymes for reduced immunological stress
CN105831428A (en) * 2005-12-15 2016-08-10 美国礼来公司 Enzymes for reduced immunological stress
NO340818B1 (en) * 2005-12-15 2017-06-26 Lilly Co Eli Compositions for oral administration to animals comprising enzymes for reduced immunological stress
CN105831428B (en) * 2005-12-15 2020-12-01 伊兰科美国公司 Enzymes for reducing immune stress
WO2010109257A1 (en) * 2009-03-23 2010-09-30 Carlo Ghisalberti Composition to treat protein intolerance
WO2014207435A1 (en) * 2013-06-25 2014-12-31 Ucl Business Plc Anti-microbial agents and uses therefor
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