GB2017906A - Improved assay method for the determination of estrogens and ready- for-use unit therefor - Google Patents

Improved assay method for the determination of estrogens and ready- for-use unit therefor

Info

Publication number
GB2017906A
GB2017906A GB7908208A GB7908208A GB2017906A GB 2017906 A GB2017906 A GB 2017906A GB 7908208 A GB7908208 A GB 7908208A GB 7908208 A GB7908208 A GB 7908208A GB 2017906 A GB2017906 A GB 2017906A
Authority
GB
United Kingdom
Prior art keywords
estrogens
ready
nadp
dehydrogenase
assay method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7908208A
Other versions
GB2017906B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Publication of GB2017906A publication Critical patent/GB2017906A/en
Application granted granted Critical
Publication of GB2017906B publication Critical patent/GB2017906B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The assay method relates to the enzymatic determination of estrogens E1 and E2, especially in plasma or urine. Said method is carried out by means of 17 beta -estradiol-dehydrogenase of placental origin, desteriodated and freed from interfering non-estrogeno-dependent transhydrogenases, in the presence of NAD or an analogue of NAD recognized by the enzyme, of NADP or of one of its analogues, of a specific NADP- dehydrogenase enzyme and of a NADP- generating substrate. It may be performed by means of a ready-for-use unit or kit comprising essentially the above components in suitable predetermined proportions, in combination with an internal standard, a blank and buffer media. 17 beta -estradiol dehydrogenase is extracted from human placenta.
GB7908208A 1978-03-09 1979-03-08 Assay method for the determination of estrogens and ready-for-use unit therefor Expired GB2017906B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7806807A FR2419519A1 (en) 1978-03-09 1978-03-09 IMPROVEMENTS TO THE METHODS FOR MEASURING EESTROGENS AND READY TO USE ASSEMBLY FOR THE PERFORMANCE OF THE SAID DOSES

Publications (2)

Publication Number Publication Date
GB2017906A true GB2017906A (en) 1979-10-10
GB2017906B GB2017906B (en) 1983-02-23

Family

ID=9205562

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7908208A Expired GB2017906B (en) 1978-03-09 1979-03-08 Assay method for the determination of estrogens and ready-for-use unit therefor

Country Status (3)

Country Link
DE (1) DE2908957A1 (en)
FR (1) FR2419519A1 (en)
GB (1) GB2017906B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL57135A (en) * 1979-04-25 1982-12-31 Abic Project Partnership Method for the determination of a factor or factors which enhances the cortisol metabolism in lymphocytes or leucocytes
FR2547926B1 (en) * 1983-06-27 1986-05-23 Biomerieux Sa ASSAY PROCESS FOR ESTROGENS AND ANDROGENS BY AMPLIFICATION AND BIOLUMINESCENCE

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1397406A (en) * 1971-06-22 1975-06-11 Nyegaard & Co As Enzymic reagent

Also Published As

Publication number Publication date
DE2908957C2 (en) 1990-05-17
FR2419519A1 (en) 1979-10-05
DE2908957A1 (en) 1979-09-27
GB2017906B (en) 1983-02-23
FR2419519B1 (en) 1981-08-14

Similar Documents

Publication Publication Date Title
Garland et al. Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-coenzyme A in rat-liver mitochondria oxidizing palmitate
EP0179486A3 (en) Process for producing peroxidase
FR2446406A1 (en) EXPANDABLE RIVET ARRANGEMENT
ATE5898T1 (en) PROCEDURE FOR ENZYMATIC GLUCOSE OXIDATION.
DE2964377D1 (en) Analytical process and means for measuring the amount of hydrogen peroxide in aqueous media and of organic substrates generating hydrogen peroxide by enzymatic oxidation
GB1295992A (en)
GB1395126A (en) Process and reagent for the determination of triglycerides
ES8708163A1 (en) Miniaturized yeast identification system.
ES8106934A1 (en) A composition, test device and method of making it, and method for the determination of an analyte in a liquid.
GB1516820A (en) Process and reagent for determining total and bound cholesterol
GB1502204A (en) Process and reagent for the determination of triglyceride
GB2017906A (en) Improved assay method for the determination of estrogens and ready- for-use unit therefor
JPS55110711A (en) Desulfurization of molten pig iron
AU6879587A (en) Composition and method for stabilization of dinucleotides
Mason et al. INHIBITION OF PYRIDOXAL PHOSPHATE-DEPENDENT ENZYMES BY THE SULFATE ESTERS OF ESTRADIOL, ESTRONE AND DIETHYLSTILBESTROL1
GB1395127A (en) Process for the quantitative photometric determination of uric acid
ES486658A1 (en) Enzymatic bating method
White et al. Studies on adrenocorticotropin. XIV. Action of bovine fibrinolysin and of liver cathepsin on corticotropin-A: Effect on biological activity
CHIAO et al. Characterization of rat testicular alcohol dehydrogenase
GB1457678A (en) Determination of serum triglycerides and reagents therefor
GB1104197A (en) Method of producing uricase from yeast
EP0882800A3 (en) Method for determination of direct bilirubin and reagent therefor
FR2389892A1 (en) Colorimetric assay of total bile acid content - of blood, urine or bile, by reducing tetrazolium salt to formazan
JPS561896A (en) Determination of hydrogen peroxide and reagent for it
DE2961445D1 (en) Delta 5,3-cetosteroid isomerase and its use in immuno-enzyme quantifications

Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19950308