GB1395126A - Process and reagent for the determination of triglycerides - Google Patents

Process and reagent for the determination of triglycerides

Info

Publication number
GB1395126A
GB1395126A GB2769573A GB2769573A GB1395126A GB 1395126 A GB1395126 A GB 1395126A GB 2769573 A GB2769573 A GB 2769573A GB 2769573 A GB2769573 A GB 2769573A GB 1395126 A GB1395126 A GB 1395126A
Authority
GB
United Kingdom
Prior art keywords
saponification
lipase
determination
glycerol
carboxylesterase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2769573A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2229849A external-priority patent/DE2229849C2/en
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1395126A publication Critical patent/GB1395126A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1395126 Determination of glycerides BOEHRINGER MANNHEIM GmbH 11 June 1973 [19 June 1972] 27695/73 Heading G1B A process for the determination of triglycerides by enzymatic saponification with lipase and measurement of the liberated glycerol, is characterized in that the saponification is carried out in the presence of carboxylesterase (such as pig liver, esterase) and of an alkali metal or alkaline earth metal alkyl sulphate, the alkyl radical of which contains 10 to 15 carbon atoms. Optionally serum albumen such as bovine serum albumen is present, and cobalt and/or magnesium ions may be included to accelerate the reaction. Preferably the lipase used is from Rhizopus arrhizus. The invention also comprises the reagent for carrying out the above process and comprising a saponification agent, a buffer and a system for the detection of glycerol, wherein the saponification agent comprises a lipase, carboxylesterase and an alkali metal or alkaline earth metal alkyl sulphate, the alkyl radical of which contains 10 to 15 carbon atoms. A suitable glycerol detection system comprises NADH, ATP, phosphoenol pyruvate, lactate dehydrogenase, pyruvate kinase, glycerokinase, Co and/or Mg ions and a buffer. The process finds application in foodstuff analysis and medical diagnosis.
GB2769573A 1972-06-19 1973-06-11 Process and reagent for the determination of triglycerides Expired GB1395126A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2229849A DE2229849C2 (en) 1972-06-19 1972-06-19 Method and reagent for the determination of triglycerides

Publications (1)

Publication Number Publication Date
GB1395126A true GB1395126A (en) 1975-05-21

Family

ID=5848134

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2769573A Expired GB1395126A (en) 1972-06-19 1973-06-11 Process and reagent for the determination of triglycerides

Country Status (17)

Country Link
US (1) US3862009A (en)
JP (1) JPS4964495A (en)
AR (1) AR195328A1 (en)
AT (1) AT324282B (en)
BE (1) BE801106A (en)
CA (1) CA994658A (en)
CH (1) CH573117A5 (en)
CS (1) CS166842B2 (en)
DD (1) DD104367A5 (en)
DK (1) DK144643C (en)
FR (1) FR2190277A5 (en)
GB (1) GB1395126A (en)
HU (1) HU167097B (en)
IT (1) IT990535B (en)
NL (1) NL165845C (en)
SE (1) SE413326B (en)
SU (1) SU639487A3 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275152A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4275151A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT339505B (en) * 1974-03-14 1977-10-25 Boehringer Mannheim Gmbh ENZYMATIC ANALYSIS PROCEDURE
JPS50142090A (en) * 1974-04-30 1975-11-15
US4014744A (en) * 1975-01-30 1977-03-29 Miles Laboratories Inc. Processes for measuring tri-, di- and monoglycerides
AT354406B (en) * 1975-08-12 1979-01-10 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR DETERMINATION OF TRIGLYCERIDES
US4012287A (en) * 1975-11-18 1977-03-15 Dr. Bruno Lange Gmbh Method and reagent for the quantitative analysis of triglycerides
US4045297A (en) * 1975-12-15 1977-08-30 Monsanto Company Triglycerides determination method
DE2558536B2 (en) * 1975-12-24 1979-07-12 Boehringer Mannheim Gmbh, 6800 Mannheim Method of kinetic substrate determination and reagent for its implementation
US4056442A (en) * 1976-06-01 1977-11-01 The Dow Chemical Company Lipase composition for glycerol ester determination
US4179334A (en) * 1976-08-19 1979-12-18 Eastman Kodak Company Hydrolysis of protein-bound triglycerides
US4241178A (en) * 1978-01-06 1980-12-23 Eastman Kodak Company Process and composition for the quantification of glycerol ATP and triglycerides
DE2831580C2 (en) * 1978-07-18 1980-09-18 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for the determination of glycerin
US4178285A (en) * 1978-12-20 1979-12-11 Felts James M Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
US4264589A (en) * 1978-12-20 1981-04-28 Felts James M Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
FR2449726A1 (en) * 1979-02-22 1980-09-19 Millipore Corp Fast reacting lipase compsn. for hydrolysis of glycerol ester(s) - contg. Rhizopus arrhizus lipase and Pseudomonas Fluoresens lipase
US4394445A (en) * 1979-02-22 1983-07-19 Nix Paul T Enzymatic glyceride hydrolysis
US4259440A (en) * 1979-05-21 1981-03-31 Miles Laboratories, Inc. Hydrolysis and assay of triglycerides
JPS55156598A (en) * 1979-05-25 1980-12-05 Mitsubishi Chem Ind Ltd Enzymatic determination of monobasic fatty acid
JPS561895A (en) * 1979-06-20 1981-01-10 Mitsubishi Chem Ind Ltd Enzymic determination of monofunctional fatty acid
DE2950381A1 (en) * 1979-12-14 1981-06-19 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD AND REAGENT FOR DETERMINING TRIGLYCERIDES
US4322496A (en) * 1980-04-17 1982-03-30 Eastman Kodak Company Inhibition of lactate oxidase
US4309502A (en) * 1980-06-30 1982-01-05 Beckman Instruments, Inc. Enzymatic assay for glycerol and triglycerides and a reagent for use therein
EP0045031A1 (en) * 1980-07-22 1982-02-03 Baker Instruments Corporation Glycerol detection reagent and its use
DE3413118A1 (en) * 1984-04-06 1985-10-24 Miles Laboratories, Inc., Elkhart, Ind. ANALYSIS METHOD AND MEANS FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYMS

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2000127C3 (en) * 1970-01-02 1974-12-12 Boehringer Mannheim Gmbh Method for the quantitative cleavage and for the quantitative detection of tri-, di- and monoglycerides
US3703591A (en) * 1970-12-16 1972-11-21 Calbiochem Triglyceride hydrolysis and assay

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275152A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4275151A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters

Also Published As

Publication number Publication date
NL165845C (en) 1981-05-15
HU167097B (en) 1975-08-28
NL7305350A (en) 1973-12-21
CH573117A5 (en) 1976-02-27
DK144643C (en) 1982-09-27
DK144643B (en) 1982-04-26
SE413326B (en) 1980-05-19
CS166842B2 (en) 1976-03-29
JPS4964495A (en) 1974-06-21
CA994658A (en) 1976-08-10
SU639487A3 (en) 1978-12-25
US3862009A (en) 1975-01-21
AR195328A1 (en) 1973-09-28
AT324282B (en) 1975-08-25
DD104367A5 (en) 1974-03-05
NL165845B (en) 1980-12-15
FR2190277A5 (en) 1974-01-25
BE801106A (en) 1973-12-19
IT990535B (en) 1975-07-10

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years

Effective date: 19930610