GB1598019A - Bacteriophage and method for preparing same - Google Patents
Bacteriophage and method for preparing same Download PDFInfo
- Publication number
- GB1598019A GB1598019A GB16242/78A GB1624278A GB1598019A GB 1598019 A GB1598019 A GB 1598019A GB 16242/78 A GB16242/78 A GB 16242/78A GB 1624278 A GB1624278 A GB 1624278A GB 1598019 A GB1598019 A GB 1598019A
- Authority
- GB
- United Kingdom
- Prior art keywords
- phage
- bacteriophage
- dna
- endonuclease
- ifo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241001515965 unidentified phage Species 0.000 title claims description 41
- 238000000034 method Methods 0.000 title claims description 29
- 108020004414 DNA Proteins 0.000 claims description 73
- 102000053602 DNA Human genes 0.000 claims description 64
- 108091008146 restriction endonucleases Proteins 0.000 claims description 33
- 230000002068 genetic effect Effects 0.000 claims description 31
- 101710132601 Capsid protein Proteins 0.000 claims description 29
- 101710094648 Coat protein Proteins 0.000 claims description 29
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 29
- 101710125418 Major capsid protein Proteins 0.000 claims description 29
- 101710141454 Nucleoprotein Proteins 0.000 claims description 29
- 101710083689 Probable capsid protein Proteins 0.000 claims description 29
- 108010042407 Endonucleases Proteins 0.000 claims description 24
- 102000004533 Endonucleases Human genes 0.000 claims description 24
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 claims description 12
- 230000007017 scission Effects 0.000 claims description 11
- 238000003776 cleavage reaction Methods 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000012217 deletion Methods 0.000 claims description 7
- 230000037430 deletion Effects 0.000 claims description 7
- 241000894007 species Species 0.000 claims description 6
- 238000003786 synthesis reaction Methods 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 5
- 230000013011 mating Effects 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000001243 protein synthesis Methods 0.000 claims 2
- 230000014616 translation Effects 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 37
- 239000000725 suspension Substances 0.000 description 24
- 229920001817 Agar Polymers 0.000 description 19
- 239000008272 agar Substances 0.000 description 19
- 239000002609 medium Substances 0.000 description 19
- 239000012137 tryptone Substances 0.000 description 18
- 239000002245 particle Substances 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000011777 magnesium Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 239000007853 buffer solution Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 6
- 230000006798 recombination Effects 0.000 description 6
- 238000005215 recombination Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 230000028744 lysogeny Effects 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 239000011425 bamboo Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- -1 for example Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- WZIMSXIXZTUBSO-UHFFFAOYSA-N 2-[[bis(carboxymethyl)amino]methyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CN(CC(O)=O)CC(O)=O WZIMSXIXZTUBSO-UHFFFAOYSA-N 0.000 description 1
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 238000010504 bond cleavage reaction Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007412 host metabolism Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000007505 plaque formation Effects 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- NVBFHJWHLNUMCV-UHFFFAOYSA-N sulfamide Chemical class NS(N)(=O)=O NVBFHJWHLNUMCV-UHFFFAOYSA-N 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 101150035767 trp gene Proteins 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4740977A JPS53133684A (en) | 1977-04-26 | 1977-04-26 | Novel bacteriophage and fabricating same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1598019A true GB1598019A (en) | 1981-09-16 |
Family
ID=12774319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB16242/78A Expired GB1598019A (en) | 1977-04-26 | 1978-04-25 | Bacteriophage and method for preparing same |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4332897A (enExample) |
| JP (1) | JPS53133684A (enExample) |
| DK (1) | DK161105C (enExample) |
| GB (1) | GB1598019A (enExample) |
| NL (1) | NL191440C (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0095934A3 (en) * | 1982-06-02 | 1985-08-14 | Noda Institute For Scientific Research | Novel bacteriophage and method for breeding thereof |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4469786A (en) * | 1981-01-30 | 1984-09-04 | The Mount Sinai School Of Medicine Of The City University Of New York | Detection of chemical mutagens in marker rescue assay |
| US4593002A (en) * | 1982-01-11 | 1986-06-03 | Salk Institute Biotechnology/Industrial Associates, Inc. | Viruses with recombinant surface proteins |
| US5559018A (en) * | 1983-09-13 | 1996-09-24 | Mattson; Thomas L. | Inviable phages, their production and DNA thereof |
| US4774182A (en) * | 1984-06-29 | 1988-09-27 | Wisconsin Alumni Research Foundation | Partially defective foreign gene for conferring immunity on a biological host |
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US7413537B2 (en) * | 1989-09-01 | 2008-08-19 | Dyax Corp. | Directed evolution of disulfide-bonded micro-proteins |
| AU8740491A (en) * | 1990-09-28 | 1992-04-28 | Protein Engineering Corporation | Proteinaceous anti-dental plaque agents |
| ATE439435T1 (de) * | 1991-03-01 | 2009-08-15 | Dyax Corp | Chimäres protein mit mikroprotein mit zwei oder mehr disulfidbindungen und ausgestaltungen davon |
| CA2186962A1 (en) * | 1994-04-05 | 1995-10-12 | Richard M. Carlton | Antibacterial therapy with genotypically modified bacteriophage |
| US20010043924A1 (en) * | 1994-04-05 | 2001-11-22 | Exponential Biotherapies, Inc. | Antibacterial therapy with bacteriophage physico-chemically altered to delay inactivation by the host defense system |
| NZ294014A (en) | 1994-10-18 | 1999-02-25 | Scottish Crop Research Inst | A method of producing a rod-shaped or pseudovirus capable of replicating and assembling in plants |
| US20090053179A1 (en) * | 2005-10-05 | 2009-02-26 | Internalle, Inc. | Method for using liberated dormant bacteriophage and environmental stress to reduce infectious bacteria |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4259444A (en) * | 1972-06-07 | 1981-03-31 | General Electric Company | Microorganisms having multiple compatible degradative energy-generating plasmids and preparation thereof |
-
1977
- 1977-04-26 JP JP4740977A patent/JPS53133684A/ja active Granted
-
1978
- 1978-04-12 US US05/895,602 patent/US4332897A/en not_active Expired - Lifetime
- 1978-04-25 GB GB16242/78A patent/GB1598019A/en not_active Expired
- 1978-04-25 DK DK179078A patent/DK161105C/da not_active IP Right Cessation
- 1978-04-26 NL NL7804483A patent/NL191440C/xx not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0095934A3 (en) * | 1982-06-02 | 1985-08-14 | Noda Institute For Scientific Research | Novel bacteriophage and method for breeding thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6137915B2 (enExample) | 1986-08-26 |
| NL191440B (nl) | 1995-03-01 |
| US4332897A (en) | 1982-06-01 |
| DK161105B (da) | 1991-05-27 |
| NL191440C (nl) | 1995-07-04 |
| DK161105C (da) | 1991-11-11 |
| DK179078A (da) | 1978-10-27 |
| NL7804483A (nl) | 1978-10-30 |
| JPS53133684A (en) | 1978-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4348477A (en) | Method for preparing a recombinant DNA phage | |
| Roth | [1] Genetic techniques in studies of bacterial metabolism | |
| Chetverin et al. | On the nature of spontaneous RNA synthesis by Qβ replicase | |
| Morrison et al. | Frequency of F116-mediated transduction of Pseudomonas aeruginosa in a freshwater environment | |
| US4348478A (en) | Method for preparation of a recombinant DNA phage | |
| US4332897A (en) | Novel bacteriophage and method for preparing same | |
| Belfort et al. | Genetic and biochemical investigation of the Escherichia coli mutant hfl-1 which is lysogenized at high frequency by bacteriophage lambda | |
| Duncan et al. | Transformation of Bacillus subtilis and Escherichia coli by a hybrid plasmid pCD1 | |
| Muckerman et al. | Transformation of restriction endonuclease phenotype in Streptococcus pneumoniae | |
| EP0095934B1 (en) | Novel bacteriophage and method for breeding thereof | |
| Abeliovich et al. | Bacteriophages of Rhodopseudomonas spheroides: isolation and characterization of a Rhodopseudomonas spheroides bacteriophage | |
| MOMOSE et al. | On the transducing phages in glutamic acid-producing bacteria | |
| Bamburg et al. | Progress in Molecular and Subcellular Biology | |
| Weissmann et al. | Reduction of possible hazards in the preparation of recombinant plasmid DNA | |
| JPS60153792A (ja) | 低下した制限活性を持つストレプトマイセスの宿主細胞 | |
| Hewitt | Mechanism of virulence transfer by bacterial viruses | |
| US5273902A (en) | Genetically modified vibrio | |
| US6589732B2 (en) | Transducing phages | |
| Roberts et al. | Characterization of Bdellovibrio bacteriovorus bacteriophage MAC-1 | |
| Tichý et al. | Genetic transformation and transfection of Bacillus subtilis spheroplasts | |
| CN116656623B (zh) | 两株具有特征分子靶标的蜡样芽孢杆菌广谱肌尾噬菌体dc1、dc2及其应用 | |
| Elseth | The Multiplication of Lambda and Lambda-DG Within Their Lysogenic Host Escherichia coli K12 | |
| JPS5869898A (ja) | 新規なテンペレ−トフア−ジｄｎａ | |
| Varon | The bdellophage three-membered parasitic system | |
| Ripp | The role of transduction and pseudolysogeny in bacteriophage-host interactions in a natural freshwater environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19980424 |