GB1430217A - Process for the preparation of an infusible storage-stable haemoglobin solution - Google Patents
Process for the preparation of an infusible storage-stable haemoglobin solutionInfo
- Publication number
- GB1430217A GB1430217A GB4615773A GB4615773A GB1430217A GB 1430217 A GB1430217 A GB 1430217A GB 4615773 A GB4615773 A GB 4615773A GB 4615773 A GB4615773 A GB 4615773A GB 1430217 A GB1430217 A GB 1430217A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- isotonicity
- adjusted
- erythrocytes
- stable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000243 solution Substances 0.000 abstract 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 210000003743 erythrocyte Anatomy 0.000 abstract 3
- 239000000047 product Substances 0.000 abstract 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 2
- 101100188552 Arabidopsis thaliana OCT3 gene Proteins 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- VEZXCJBBBCKRPI-UHFFFAOYSA-N beta-propiolactone Chemical compound O=C1CCO1 VEZXCJBBBCKRPI-UHFFFAOYSA-N 0.000 abstract 2
- 239000003633 blood substitute Substances 0.000 abstract 2
- 239000003729 cation exchange resin Substances 0.000 abstract 2
- 229960000380 propiolactone Drugs 0.000 abstract 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 abstract 2
- 235000017557 sodium bicarbonate Nutrition 0.000 abstract 2
- XOHUEYCVLUUEJJ-UHFFFAOYSA-I 2,3-Diphosphoglycerate Chemical compound [O-]P(=O)([O-])OC(C(=O)[O-])COP([O-])([O-])=O XOHUEYCVLUUEJJ-UHFFFAOYSA-I 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract 1
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 239000010425 asbestos Substances 0.000 abstract 1
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920002301 cellulose acetate Polymers 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 239000008151 electrolyte solution Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 235000001727 glucose Nutrition 0.000 abstract 1
- 208000006454 hepatitis Diseases 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 229910052895 riebeckite Inorganic materials 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000011146 sterile filtration Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/829—Blood
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
1430217 Blood substitute BIOTESTSERUM-INSTITUT GmbH 3 Oct 1973 [3 Oct 1972] 46157/73 Heading ASB Hepatitis-proof, storage-stable, infusible haemoglobin solution which has a stable 2, 3- diphosphoglycerate level is prepared by (a) treating human erythrocytes in dilute solution with beta-propiolactone, (b) washing the product with a neutral or weakly alkaline solution to remove excess beta-propiolactone and its reaction products, (c) haemolysing the erythrocytes by stirring them into distilled water or a physiologically acceptable electrolyte solution, (d) reducing the pH and potassium content of the haemolysate and precipitating the stromalipids by treating the haemolysate with a cation exchange resin in the H<+> form, (e) removing the resin and the precipitated stroma mass by centrifuge, (f) adding substances to regulate isotonicity and pH and optionally ferrohaemoglobin and (g) filtering the product under sterile conditions. The solution used to wash the erythrocytes may be sodium chloride or sodium bicarbonate solution. The cation-exchange resin may be a polystyrene sulphonate. The isotonicity may be adjusted by addition of glucose, sodium acetate or sodium bicarbonate. The pH may be adjusted by addition of sodium hydroxide. The sterile filtration may be through a cellulose asbestos filter or cellulose acetate disc. The solution may be concentrated before its isotonicity is adjusted by freezing it and collecting the haemoglobin-rich melt which runs off the frozen body. The product may be used as a blood substitute, particularly in cases of shock.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2248475A DE2248475C3 (en) | 1972-10-03 | 1972-10-03 | Process for obtaining hepatic-safe, storage-stable and infusable hemoglobin solutions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1430217A true GB1430217A (en) | 1976-03-31 |
Family
ID=5858076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4615773A Expired GB1430217A (en) | 1972-10-03 | 1973-10-03 | Process for the preparation of an infusible storage-stable haemoglobin solution |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3864478A (en) |
| JP (1) | JPS6025411B2 (en) |
| DE (1) | DE2248475C3 (en) |
| FR (1) | FR2201102B1 (en) |
| GB (1) | GB1430217A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5872227A (en) * | 1993-04-08 | 1999-02-16 | Gramineer Ab | Process for separation of components from red blood cells |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4001200A (en) * | 1975-02-27 | 1977-01-04 | Alza Corporation | Novel polymerized, cross-linked, stromal-free hemoglobin |
| US3991181A (en) * | 1975-06-18 | 1976-11-09 | Warner-Lambert Company | Injectable stroma free hemoglobin solution and its method of manufacture |
| GB1578776A (en) * | 1976-06-10 | 1980-11-12 | Univ Illinois | Hemoglobin liposome and method of making the same |
| US4401652A (en) * | 1980-12-31 | 1983-08-30 | Allied Corporation | Process for the preparation of stroma-free hemoglobin solutions |
| DE3130770C2 (en) * | 1981-08-04 | 1986-06-19 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | Process for obtaining hepatitis-safe, sterile, pyrogen-free and stroma-free hemoglobin solutions |
| DE3225408A1 (en) * | 1982-07-07 | 1984-01-12 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | AQUEOUS SOLUTION FOR SUSPENDING AND STORING CELLS, ESPECIALLY ERYTHROCYTES |
| DE3587351T3 (en) * | 1984-03-23 | 1998-04-16 | Baxter Int | HEMOGLOBIN WITH REDUCED VIRUS RISK AND THEIR PRODUCTION. |
| US5281579A (en) * | 1984-03-23 | 1994-01-25 | Baxter International Inc. | Purified virus-free hemoglobin solutions and method for making same |
| DE3412144A1 (en) * | 1984-03-31 | 1985-10-10 | Biotest Pharma GmbH, 6000 Frankfurt | METHOD FOR PRODUCING HIGHLY CLEANED, ELECTRICITY-FREE, HEPATITIC-SAFE HUMAN AND ANIMAL HEMOGLOBIN SOLUTIONS |
| US4908350A (en) * | 1985-10-31 | 1990-03-13 | The Regents Of The University Of California | Hyperosmotic/hyperoncotic solutions for resuscitation of hypodynamic shock |
| US5753616A (en) * | 1986-11-10 | 1998-05-19 | Biopure Corporation | Method for producing a stable polymerized hemoglobin blood-substitute |
| US5955581A (en) * | 1986-11-10 | 1999-09-21 | Biopure Corporation | Method for producing a stable polymerized hemoglobin blood-substitute |
| US4927806A (en) * | 1987-04-23 | 1990-05-22 | The Regents Of The University Of California | Saturated salt/concentrated dextran formulation to treat hemorrhage |
| US5439882A (en) * | 1989-12-29 | 1995-08-08 | Texas Tech University Health Sciences Center | Blood substitute |
| US5895810A (en) * | 1995-03-23 | 1999-04-20 | Biopure Corporation | Stable polymerized hemoglobin and use thereof |
| US5691453A (en) * | 1995-06-07 | 1997-11-25 | Biopure Corporation | Separation of polymerized hemoglobin from unpolymerized hemoglobin on hydroxyapatite using HPLC |
| US5741894A (en) * | 1995-09-22 | 1998-04-21 | Baxter International, Inc. | Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form |
| US6518010B2 (en) * | 2001-02-28 | 2003-02-11 | Biopure Corporation | Use of defibrinated blood for manufacture of a hemoglobin-based oxygen carrier |
| US7001715B2 (en) * | 2002-02-28 | 2006-02-21 | Biopure Corporation | Purification of red blood cells by separation and diafiltration |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2527210A (en) * | 1944-01-25 | 1950-10-24 | John O Bower | Hemoglobin solution and method |
| US3634290A (en) * | 1969-08-06 | 1972-01-11 | Tipton L Golias | Method of preparing hemolysates for hemoglobin and other types of electrophoresis using chelating agents |
-
1972
- 1972-10-03 DE DE2248475A patent/DE2248475C3/en not_active Expired
-
1973
- 1973-10-02 FR FR7335228A patent/FR2201102B1/fr not_active Expired
- 1973-10-03 US US403141A patent/US3864478A/en not_active Expired - Lifetime
- 1973-10-03 JP JP48110633A patent/JPS6025411B2/en not_active Expired
- 1973-10-03 GB GB4615773A patent/GB1430217A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5872227A (en) * | 1993-04-08 | 1999-02-16 | Gramineer Ab | Process for separation of components from red blood cells |
Also Published As
| Publication number | Publication date |
|---|---|
| US3864478A (en) | 1975-02-04 |
| DE2248475B2 (en) | 1978-02-23 |
| JPS6025411B2 (en) | 1985-06-18 |
| DE2248475A1 (en) | 1974-04-25 |
| FR2201102B1 (en) | 1977-01-28 |
| FR2201102A1 (en) | 1974-04-26 |
| JPS5012223A (en) | 1975-02-07 |
| DE2248475C3 (en) | 1978-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19931002 |