GB1407720A - Carrier bound macromolecular compounds - Google Patents

Carrier bound macromolecular compounds

Info

Publication number
GB1407720A
GB1407720A GB5602573A GB5602573A GB1407720A GB 1407720 A GB1407720 A GB 1407720A GB 5602573 A GB5602573 A GB 5602573A GB 5602573 A GB5602573 A GB 5602573A GB 1407720 A GB1407720 A GB 1407720A
Authority
GB
United Kingdom
Prior art keywords
epoxypropyl
compound
cross
molecular sieve
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5602573A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1407720A publication Critical patent/GB1407720A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/44—Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Polymerisation Methods In General (AREA)
  • Medicinal Preparation (AREA)
  • Macromonomer-Based Addition Polymer (AREA)

Abstract

1407720 Carrier bound macromolecular compounds BOEHRINGER MANNHEIM GmbH 4 Dec 1973 [8 Dec 1972] 56025/73 Headings C3H and C3P Carrier bound macromolecular compounds (e.g. protein, nucleic acid, peptide, porphyrin, haemin, phosphatide, cerebroside, ganglyoside or glycoside, e.g. enzymes, antibodies or hormones) covalently bound on to the surface of a polymer body which permeates a molecular sieve body are obtained by reacting a macromolecular compound A with a compound B which contains at least one function capable of coupling with the macromolecular compound A and at least one further function capable of polymerization, whereafter there is added a molecular sieve material in wholly or partially unswollen state, the degree of cross-linking of which excludes the macromolecular compound A, and the polymerizable group of the coupled product AB is then polymerized in the molecular sieve material. The compound B may comprise a group which alkylates or acylates the macromolecular compound and as the polymerizable group a carbon-carbon or carbon-oxygen double bond. The coupling function may be an oxirane, ethyleneimine, halide, acid halide, azide or anhydride group and may, for example have the formula in which R 1 is a mono- or polyunsaturated aliphatic hydrocarbon radical optionally containing one oxo group adjacent to a double bond and R 2 is a hydrogen atom or a lower alkyl radical. Compound B is preferably acryloyl chloride, allyl bromide, maleic anhydride, maleic acid azide, 1-allyloxy-3-(N-ethyleneimine) - propan - 2 - ol, acrylic acid 2,3 - epoxypropyl ester, but-2,3-ene oxide, methacrylic acid-2,3-epoxypropyl ester, maleic acid 2,3- epoxypropyl monoester, fumaric acid epoxy-(2, 3-epoxypropyl ester), fumaric acid epoxy-(2,3- epoxypropyl diester), fumaric acid epoxy-(2,3- epoxypropyl diester) or 1-allyloxy-2,3-epoxybutane. The molecular sieve material is advantageously cross-linked dextran, cross-linked polyacrylamide or cross-linked agar. In the examples glucose oxidase is coupled to an acrylamide/N,N<SP>1</SP>-methylene bis-acrylamide copolymer obtained by polymerization thereof using ammonium peroxydisulphate or to a cyanogen bromide activated cross-linked dextran gel. Poly A is attached to a polyacrylamide/N,N<SP>1</SP>- methylene bis-acrylamide copolymer.
GB5602573A 1972-12-08 1973-12-04 Carrier bound macromolecular compounds Expired GB1407720A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2260184A DE2260184C3 (en) 1972-12-08 1972-12-08 Process for the production of a carrier-fixed macromolecular compound

Publications (1)

Publication Number Publication Date
GB1407720A true GB1407720A (en) 1975-09-24

Family

ID=5863934

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5602573A Expired GB1407720A (en) 1972-12-08 1973-12-04 Carrier bound macromolecular compounds

Country Status (14)

Country Link
JP (1) JPS4999181A (en)
BE (1) BE807713A (en)
CA (1) CA1017692A (en)
CH (1) CH606066A5 (en)
DE (1) DE2260184C3 (en)
FR (1) FR2209774B1 (en)
GB (1) GB1407720A (en)
HU (1) HU169832B (en)
IL (1) IL43582A (en)
IT (1) IT1048144B (en)
NL (1) NL172969C (en)
SE (1) SE420611B (en)
SU (1) SU526294A3 (en)
ZA (1) ZA739320B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976527A (en) * 1981-08-05 1999-11-02 Siol, Werner Roehm Gmbh Chemishe Fabrik High surface area support having bound latex particles containing oxirane groups for immobilization of substances

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2426988C2 (en) * 1974-06-04 1985-02-14 Boehringer Mannheim Gmbh, 6800 Mannheim Method for carrier binding of biologically active proteins
DE2633259C3 (en) * 1975-07-23 1984-11-15 Japan Atomic Energy Research Institute, Tokio/Tokyo Method for immobilizing enzymes or enzyme-containing cells
US4210722A (en) * 1977-11-14 1980-07-01 Minnesota Mining And Manufacturing Company Protein immobilizer
US4530963A (en) * 1982-08-20 1985-07-23 Devoe-Holbein International, N.V. Insoluble chelating compositions

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH538003A (en) * 1968-03-29 1973-01-31 Anvar Process for obtaining textile articles carrying enzymes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976527A (en) * 1981-08-05 1999-11-02 Siol, Werner Roehm Gmbh Chemishe Fabrik High surface area support having bound latex particles containing oxirane groups for immobilization of substances

Also Published As

Publication number Publication date
CA1017692A (en) 1977-09-20
CH606066A5 (en) 1978-10-13
DE2260184B2 (en) 1981-01-22
ZA739320B (en) 1974-11-27
IL43582A0 (en) 1974-03-14
DE2260184C3 (en) 1981-10-08
IT1048144B (en) 1980-11-20
JPS4999181A (en) 1974-09-19
IL43582A (en) 1976-12-31
NL172969C (en) 1983-11-16
SU526294A3 (en) 1976-08-25
NL7314868A (en) 1974-06-11
HU169832B (en) 1977-02-28
SE420611B (en) 1981-10-19
FR2209774B1 (en) 1977-08-05
DE2260184A1 (en) 1974-07-11
BE807713A (en) 1974-05-24
NL172969B (en) 1983-06-16
FR2209774A1 (en) 1974-07-05

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee