GB1343703A - Method of chemically coupling biologically active substances to oxirane-containing polymers - Google Patents

Method of chemically coupling biologically active substances to oxirane-containing polymers

Info

Publication number
GB1343703A
GB1343703A GB2018271A GB2018271A GB1343703A GB 1343703 A GB1343703 A GB 1343703A GB 2018271 A GB2018271 A GB 2018271A GB 2018271 A GB2018271 A GB 2018271A GB 1343703 A GB1343703 A GB 1343703A
Authority
GB
United Kingdom
Prior art keywords
trypsin
polyacrylamide
amino acid
agarose
chemically coupling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2018271A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exploaterings TBF AB
Original Assignee
Exploaterings TBF AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exploaterings TBF AB filed Critical Exploaterings TBF AB
Publication of GB1343703A publication Critical patent/GB1343703A/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1343703 Chemically coupling proteins &c. to polyacrylamide or polysaccharides EXPLOATERINGS AB TBF 19 April 1971 [23 Jan 1970] 20182/71 Headings C3H C3P and C3U A method of chemically coupling an amino acid, peptide, protein, plasma protein or nucleotide to a water-insoluble polysaccharide (agar, cellulose, dextran or cross-linked dextran) or polyacrylamide involves reacting a waterinsoluble hydrophilic oxirane group-containing form of the polysaccharide or polyacrylamide with a solution of the amino acid &c. under conditions which at least partially retain the biological activity of the amino acid &c. in the coupled product. According to the Examples (1) agarose is activated with epichlorohydrin, and soyabean-trypsin-inhibitor or trypsin is coupled to the activated agarose, (2) and (3) the coupled product is used to purify trypsin and soyabean-trypsin-inhibitor, respectively, by adsorption and elution. Examples 4, 5 and 6 are broadly similar except that the polymer is obtained by activating the tosyl ester of cellulose with sodium methylate, agarose with epibromohydrin and cross-linked polyacrylamide with bis-epoxide, respectively.
GB2018271A 1970-01-23 1971-04-19 Method of chemically coupling biologically active substances to oxirane-containing polymers Expired GB1343703A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7000843A SE394820B (en) 1970-01-23 1970-01-23 MAKE BULLY COOLANTLY BINDING TO A WATEROLO POLYMER COOLANTLY ACTIVATELY, AS AMINO ACIDS, PEPTIDES, PROTEINS, NUCLEOTIDES AND NUCLEIC ACIDS BY REACTION WITH AN SUCH POLY

Publications (1)

Publication Number Publication Date
GB1343703A true GB1343703A (en) 1974-01-16

Family

ID=20257189

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2018271A Expired GB1343703A (en) 1970-01-23 1971-04-19 Method of chemically coupling biologically active substances to oxirane-containing polymers

Country Status (7)

Country Link
JP (1) JPS5623588B1 (en)
CA (1) CA1000687A (en)
DE (1) DE2102514B2 (en)
FR (1) FR2077297B1 (en)
GB (1) GB1343703A (en)
NL (1) NL7100902A (en)
SE (1) SE394820B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987234A (en) * 1988-08-17 1991-01-22 Hoechst Aktiengesellschaft Process for the preparation of cyclic ureas
CN111965341A (en) * 2020-08-10 2020-11-20 武汉菲恩生物科技有限公司 Dextran-based HRP-goat anti-mouse IgG labeled antibody and preparation method thereof

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844892A (en) * 1972-12-19 1974-10-29 Gulf Research Development Co Method of immobilizing enzymes
FR2450263A1 (en) * 1979-02-28 1980-09-26 Dow Chemical Co Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing
DE3344912A1 (en) * 1983-12-13 1985-06-20 Hoechst Ag, 6230 Frankfurt CROSSLINKED POLYMERISATES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE
JPS61191700A (en) * 1984-12-28 1986-08-26 ジエネツクス・コ−ポレイシヨン Fixation of biological substance with epoxy-polyalkyleneimine copolymer
DE3500180A1 (en) * 1985-01-04 1986-07-10 Ernst Prof. Dr. 7400 Tübingen Bayer Graft copolymers from crosslinked polymers and polyoxyethylene, process for their preparation and their use
US4882226A (en) * 1986-09-23 1989-11-21 Akzo N.V. Carrier material for use in chromatography or carrying out enzymatic reactions
US5278303A (en) * 1992-06-12 1994-01-11 University Of Toronto Innovations Foundation Polymer-supported solution synthesis of oligosaccharides
US5616698A (en) * 1994-01-10 1997-04-01 University Of Toronto Innovations Foundation Polymer-supported solution synthesis of oligosaccharides
US9180206B2 (en) * 2007-05-14 2015-11-10 Empire Technology Development Llc Pharmaceutical preparation and manufacturing method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987234A (en) * 1988-08-17 1991-01-22 Hoechst Aktiengesellschaft Process for the preparation of cyclic ureas
CN111965341A (en) * 2020-08-10 2020-11-20 武汉菲恩生物科技有限公司 Dextran-based HRP-goat anti-mouse IgG labeled antibody and preparation method thereof
CN111965341B (en) * 2020-08-10 2023-04-11 武汉菲恩生物科技有限公司 Dextran-based HRP-goat anti-mouse IgG labeled antibody and preparation method thereof

Also Published As

Publication number Publication date
NL7100902A (en) 1971-07-27
JPS5623588B1 (en) 1981-06-01
SE394820B (en) 1977-07-11
DE2102514B2 (en) 1977-08-18
CA1000687A (en) 1976-11-30
FR2077297B1 (en) 1976-03-19
DE2102514A1 (en) 1971-07-29
FR2077297A1 (en) 1971-10-22

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years