FR2450263A1 - Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing - Google Patents

Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing

Info

Publication number
FR2450263A1
FR2450263A1 FR7905248A FR7905248A FR2450263A1 FR 2450263 A1 FR2450263 A1 FR 2450263A1 FR 7905248 A FR7905248 A FR 7905248A FR 7905248 A FR7905248 A FR 7905248A FR 2450263 A1 FR2450263 A1 FR 2450263A1
Authority
FR
France
Prior art keywords
esp
gps
latex particles
monomer
pregnancy testing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7905248A
Other languages
French (fr)
Other versions
FR2450263B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dow Chemical Co
Original Assignee
Dow Chemical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Chemical Co filed Critical Dow Chemical Co
Priority to FR7905248A priority Critical patent/FR2450263A1/en
Publication of FR2450263A1 publication Critical patent/FR2450263A1/en
Application granted granted Critical
Publication of FR2450263B1 publication Critical patent/FR2450263B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Coupling of protein (I) having a reactive gp. which includes a labile H atom., to latex particles of dia. 0.15-1.5 mu comprises reacting at pH 7-9 to form covalent bonds between (I) and free epoxy gps. in the lates. Unfixed (I) is then removed. The latex particles comprise a core, made by (co)polymerising >=1 'hard' monomer, and an outer sheel made by copolymerising >=1 'hard' monomer and an ethylenically unsatd. monomer having free epoxy gps. Both core and shell may include a 'soft' comonomer. Specifically (I) includes NH2 gps., esp. it is human chorionic gonadotropin (HCG), insulin or an enzyme, and the latex is esp. styrene-glycidyl methacrylate. The conjugates are useful in antigen-antibody testing, esp. pregnancy testing. Severe conditions which might denature (I) are avoided; the particles are of uniform size distribution, so are partic. suitable for automated analysis.
FR7905248A 1979-02-28 1979-02-28 Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing Granted FR2450263A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7905248A FR2450263A1 (en) 1979-02-28 1979-02-28 Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7905248A FR2450263A1 (en) 1979-02-28 1979-02-28 Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing

Publications (2)

Publication Number Publication Date
FR2450263A1 true FR2450263A1 (en) 1980-09-26
FR2450263B1 FR2450263B1 (en) 1985-03-15

Family

ID=9222593

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7905248A Granted FR2450263A1 (en) 1979-02-28 1979-02-28 Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing

Country Status (1)

Country Link
FR (1) FR2450263A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0234083A2 (en) * 1986-02-25 1987-09-02 Tosoh Corporation Carrier for a biologically active component for immunoassay or enzymatic reaction
EP0291390A1 (en) * 1987-05-11 1988-11-17 Rhone-Poulenc Chimie Process for the preparation of polymer particles or of a latex made of polymer particles having on their surface amphiphile molecules with inorganic or reactive groups
CN113677993A (en) * 2019-03-29 2021-11-19 积水医疗株式会社 Immunoassay reagent and immunoassay method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2077297A1 (en) * 1970-01-23 1971-10-22 Exploaterings Ab Tbf
US3844392A (en) * 1971-11-02 1974-10-29 Bosch Gmbh Robert One-way clutch arrangement
FR2321520A1 (en) * 1975-08-22 1977-03-18 Rhone Poulenc Ind LATEX FROM POLYMERS WITH CN-GROUPS
FR2332287A1 (en) * 1975-11-22 1977-06-17 Behringwerke Ag COMBINATIONS WITH BIOLOGICAL ACTIVITY, THEIR PREPARATION PROCESS
US4070348A (en) * 1973-07-25 1978-01-24 Rohm Gmbh Water-swellable, bead copolymer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2077297A1 (en) * 1970-01-23 1971-10-22 Exploaterings Ab Tbf
US3844392A (en) * 1971-11-02 1974-10-29 Bosch Gmbh Robert One-way clutch arrangement
US4070348A (en) * 1973-07-25 1978-01-24 Rohm Gmbh Water-swellable, bead copolymer
FR2321520A1 (en) * 1975-08-22 1977-03-18 Rhone Poulenc Ind LATEX FROM POLYMERS WITH CN-GROUPS
FR2332287A1 (en) * 1975-11-22 1977-06-17 Behringwerke Ag COMBINATIONS WITH BIOLOGICAL ACTIVITY, THEIR PREPARATION PROCESS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DIE ANGEWANDTE MAKROMOLEKULARE CHEMIE, vol. 72, no. 72, octobre 1978 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0234083A2 (en) * 1986-02-25 1987-09-02 Tosoh Corporation Carrier for a biologically active component for immunoassay or enzymatic reaction
EP0234083A3 (en) * 1986-02-25 1989-11-08 Tosoh Corporation Carrier for a biologically active component for immunoassay or enzymatic reaction
EP0291390A1 (en) * 1987-05-11 1988-11-17 Rhone-Poulenc Chimie Process for the preparation of polymer particles or of a latex made of polymer particles having on their surface amphiphile molecules with inorganic or reactive groups
FR2615192A1 (en) * 1987-05-11 1988-11-18 Rhone Poulenc Chimie PROCESS FOR THE PREPARATION OF PARTICLES OF POLYMER OR LATEX CONSISTING OF PARTICLES OF POLYMER COMPRISING, IMPLANTED AT THEIR SURFACE, AMPHIPHILIC MOLECULES CARRYING IONOGENIC OR REACTIVE GROUPS
CN113677993A (en) * 2019-03-29 2021-11-19 积水医疗株式会社 Immunoassay reagent and immunoassay method

Also Published As

Publication number Publication date
FR2450263B1 (en) 1985-03-15

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