IT1166636B - Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing - Google Patents
Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testingInfo
- Publication number
- IT1166636B IT1166636B IT2020579A IT2020579A IT1166636B IT 1166636 B IT1166636 B IT 1166636B IT 2020579 A IT2020579 A IT 2020579A IT 2020579 A IT2020579 A IT 2020579A IT 1166636 B IT1166636 B IT 1166636B
- Authority
- IT
- Italy
- Prior art keywords
- esp
- gps
- latex particles
- monomer
- pregnancy testing
- Prior art date
Links
- 239000004816 latex Substances 0.000 title abstract 4
- 229920000126 latex Polymers 0.000 title abstract 4
- 239000002245 particle Substances 0.000 title abstract 4
- 239000004593 Epoxy Substances 0.000 title abstract 3
- 230000008878 coupling Effects 0.000 title abstract 2
- 238000010168 coupling process Methods 0.000 title abstract 2
- 238000005859 coupling reaction Methods 0.000 title abstract 2
- 230000035935 pregnancy Effects 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 230000001900 immune effect Effects 0.000 title 1
- 239000000178 monomer Substances 0.000 abstract 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 abstract 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 abstract 2
- 229940084986 human chorionic gonadotropin Drugs 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 230000007775 late Effects 0.000 abstract 1
- YPNZYYWORCABPU-UHFFFAOYSA-N oxiran-2-ylmethyl 2-methylprop-2-enoate;styrene Chemical compound C=CC1=CC=CC=C1.CC(=C)C(=O)OCC1CO1 YPNZYYWORCABPU-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Coupling of protein (I) having a reactive gp. which includes a labile H atom., to latex particles of dia. 0.15-1.5 mu comprises reacting at pH 7-9 to form covalent bonds between (I) and free epoxy gps. in the lates. Unfixed (I) is then removed. The latex particles comprise a core, made by (co)polymerising >=1 'hard' monomer, and an outer sheel made by copolymerising >=1 'hard' monomer and an ethylenically unsatd. monomer having free epoxy gps. Both core and shell may include a 'soft' comonomer. Specifically (I) includes NH2 gps., esp. it is human chorionic gonadotropin (HCG), insulin or an enzyme, and the latex is esp. styrene-glycidyl methacrylate. The conjugates are useful in antigen-antibody testing, esp. pregnancy testing. Severe conditions which might denature (I) are avoided; the particles are of uniform size distribution, so are partic. suitable for automated analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2020579A IT1166636B (en) | 1979-02-15 | 1979-02-15 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2020579A IT1166636B (en) | 1979-02-15 | 1979-02-15 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT7920205A0 IT7920205A0 (en) | 1979-02-15 |
| IT1166636B true IT1166636B (en) | 1987-05-05 |
Family
ID=11164736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT2020579A IT1166636B (en) | 1979-02-15 | 1979-02-15 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1166636B (en) |
-
1979
- 1979-02-15 IT IT2020579A patent/IT1166636B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| IT7920205A0 (en) | 1979-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4210723A (en) | Method of coupling a protein to an epoxylated latex | |
| US4480042A (en) | Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays | |
| US3876504A (en) | Procedure for determination of antigens and antibodies and articles for use therewith | |
| DE68907372D1 (en) | DIAGNOSTIC SET AND METHOD FOR THE QUICK DETERMINATION OF ANTIBODY. | |
| CA2003508A1 (en) | Monodisperse superparamagnetic particles for use in nucleic acid analyses | |
| JP3363166B2 (en) | Method of using peptide pairs with extremely high specific affinity for each other in the field of in vitro diagnostics | |
| EP0223349A3 (en) | Hybridoma continuous cell line producing a monoclonal antibody for progesterone | |
| DK0564494T3 (en) | Test method and reagent kit thereto | |
| ES8304314A1 (en) | A method and a kit for the assay of antibodies to soluble antigens. | |
| EP0974671A8 (en) | Method for diagnosing bone dysbolism | |
| AU609721B2 (en) | Dispersion polymers, biologically active dispersion polymers, a process for their preparation, and their use as diagnostic aids | |
| FR2736642B1 (en) | IMMUNOVECTORS, IN PARTICULAR ANTIBODIES AND FRAGMENTS OF ANTIBODIES FOR USE IN THE INTRACELLULAR AND INTRANUCLEAR TRANSPORT OF BIOLOGICALLY ACTIVE PRINCIPLES IN PARTICULAR OF HAPTENES, PROTEINS AND NUCLEIC ACIDS | |
| FR2450263A1 (en) | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing | |
| EP0869177A3 (en) | Human a- and b- platelet-derived growth factor receptor proteins and uses therefor. | |
| IT1166636B (en) | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing | |
| WO1990009594A3 (en) | Wash composition, test kit and their use to determine a herpes simplex viral antigen | |
| GB2005275A (en) | Immunological diagnostic reagent | |
| EP0641385A4 (en) | Development of dna probes and immunological reagents of human tumor associated antigens. | |
| EP0387027A3 (en) | Endometriosis diagnosis methods and reagents | |
| EP0348174A3 (en) | Sperm antibody test | |
| EP0229723A3 (en) | Modified beta 2-microglobulin | |
| EP0325723A3 (en) | Method for the determination of blood clotting factors and reagent for use therein | |
| CA2138536A1 (en) | Chemiluminescent Assay for dsDNA Antibodies | |
| Nagasaki et al. | Identification of red tide algae Chattonella marina and Chattonella antigua by means of monoclonal antibodies. | |
| GB2041940A (en) | Method of Coupling a Protein to an Epoxylated Latex and the Products Formed Therefrom |