GB1319114A - Microbiological method of preparing lipids - Google Patents

Microbiological method of preparing lipids

Info

Publication number
GB1319114A
GB1319114A GB5240170A GB1319114DA GB1319114A GB 1319114 A GB1319114 A GB 1319114A GB 5240170 A GB5240170 A GB 5240170A GB 1319114D A GB1319114D A GB 1319114DA GB 1319114 A GB1319114 A GB 1319114A
Authority
GB
United Kingdom
Prior art keywords
per cent
lipids
efficient
phosphorus
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5240170A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VNII BIOSINTEZA BELKOVYKH VESC
Original Assignee
VNII BIOSINTEZA BELKOVYKH VESC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VNII BIOSINTEZA BELKOVYKH VESC filed Critical VNII BIOSINTEZA BELKOVYKH VESC
Publication of GB1319114A publication Critical patent/GB1319114A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides

Abstract

1319114 Yeast &c. VSESOJUZNY NAUCHNOISSLEDOVATELSKY INSTITUT BIOSINTEZA BELKOVYKH VESCHESTV 4 Nov 1970 52401 /70 Heading C6F [Also in Division C5] A method of preparing a lipid comprises avokically cultivating lipid-producing microorganisms, particularly yeasts of the genus Candida, in a nutrient medium containing mineral salts as sources of nitrogen, phosphorus, potassium and magnesium, and a paraffinic hydrocarbon as a source of carbon, the quantity of paraffinic hydrocarbons not exceeding 75 per cent by volume, maintaining the ratio of nitrogen to carbon no lower than 1:75, and the ratio of phosphorus to carbon, calculating phosphorus as phosphorus pentexide, no lower than 1:60, and maintaining simultaneously a medium dilution co-efficient in the range of 0À5 to 0À07 per hour. The hydrocarbon may be a petroleum paraffin fraction or a synthetic paraffin. Lipids which contain up to 50% by weight phospho-lipids, up to 40 per cent free fatty acids and up to 10 per cent tri-glycerides are obtained when the amount of paraffinic hydrocarbon in the cultivation does not exceed 25 per cent by volume. N:C is from 1:5 to 1:20, P:C is from 1:15 to 1:30 and the medium dilution co-efficient is between 0À1 and 0À07 per hour. Lipids containing approximately up to 75 per cent of tri-glycerides, up to 9 per cent phospho-lipids and up to 5 per cent free fatty acids are obtained when the amount of paraffinic hydrocarbon in the cultivation does not exceed 50 per cent by volume, N:C is from 1:30 to 1:45, P:C is from 1:15 to 1:30 and the medium dilution co-efficient is between 0À1 and 0À07 per hour. Similarly, lipids which contain up to 50 per cent of free fatty acids and have only low contents of phospho-lipids and tri-glycerides are obtained when the amount of paraffinic hydrocarbon is in the range 50 to 70 per cent by volume, N:C is from 1:5 to 1:20, P:C is from 1:15 to 1:30 and the dilution co-efficient is between 0À5 and 0À07 per hour. Examples are directed to the cultivation of Candida tropicalis, Candida pelliculosa and Candida guilliermondii, respectively.
GB5240170A 1970-11-04 1970-11-04 Microbiological method of preparing lipids Expired GB1319114A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB5240170 1970-11-04

Publications (1)

Publication Number Publication Date
GB1319114A true GB1319114A (en) 1973-06-06

Family

ID=10463786

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5240170A Expired GB1319114A (en) 1970-11-04 1970-11-04 Microbiological method of preparing lipids

Country Status (1)

Country Link
GB (1) GB1319114A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4678748A (en) * 1981-11-03 1987-07-07 Pal Sutka Process for the production of immunobiological preparations applicable in the diagnosis, prevention and/or treatment of Candida guilliermondii infections
WO2005004888A1 (en) * 2003-07-04 2005-01-20 Norferm Da Lipids from methanotrophic bacteria for cholesterol reduction
EP2245136A1 (en) * 2008-01-18 2010-11-03 Bio Processing Australia Pty Ltd Process for preparing nutritional, therapeutic or organoleptic products from crude glycerol

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4678748A (en) * 1981-11-03 1987-07-07 Pal Sutka Process for the production of immunobiological preparations applicable in the diagnosis, prevention and/or treatment of Candida guilliermondii infections
WO2005004888A1 (en) * 2003-07-04 2005-01-20 Norferm Da Lipids from methanotrophic bacteria for cholesterol reduction
EP2245136A1 (en) * 2008-01-18 2010-11-03 Bio Processing Australia Pty Ltd Process for preparing nutritional, therapeutic or organoleptic products from crude glycerol
EP2245136A4 (en) * 2008-01-18 2011-06-29 Bio Proc Australia Pty Ltd Process for preparing nutritional, therapeutic or organoleptic products from crude glycerol
US8802408B2 (en) 2008-01-18 2014-08-12 Bio Processing Australia Pty Ltd. Process for preparing nutritional, therapeutic or organoleptic products from crude glycerol

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee