GB1309781A - Method for fractionating sperm - Google Patents
Method for fractionating spermInfo
- Publication number
- GB1309781A GB1309781A GB1702670A GB1702670A GB1309781A GB 1309781 A GB1309781 A GB 1309781A GB 1702670 A GB1702670 A GB 1702670A GB 1702670 A GB1702670 A GB 1702670A GB 1309781 A GB1309781 A GB 1309781A
- Authority
- GB
- United Kingdom
- Prior art keywords
- sperm
- column
- fractions
- columns
- density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Zoology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
Abstract
1309781 Fractionating sperm W SHRIMPTON 9 April 1970 [10 April 1969] 17026/70 Heading B1D [Also in Division C6] Sperm is fractionated into the female-producing X sperm and male-producing Y sperm by cooling a mixture of the sperm and a nutrient medium to a temperature of about 0.8C to immobilize the sperm and introducing the cooled mixture into a column of separating medium comprising nutrient medium, to allow separation of the sperm to different levels. A uniform density gradient of separation medium e.g. milk and cream, in the columns 35, Fig. 5, is achieved by combining progressively increasing/decreasing proportions of two liquids of different densities from containers 21 and 22, Fig. 5, by raising/lowering the containers relative to each other by means of a pulley and cable. Mixed liquid of increasing density is introduced into the columns 34 upwardly from the bottom. Further liquid of slightly greater density is then added from container 35. The cooled mixture of sperm and nutrient medium is dispensed from a refrigerated container 47 into spouts 48 and valves 46 are operated to introduce the sperm into the column at the point 44 of average or median density, corresponding to the sperm sample density. X sperm fractions are withdrawn from the bottom of the columns through stopcocks 43. Y sperm fractions may be recovered by draining the entire column or directly, from the top of the column, by means of a pipette. Fractionation efficiencies can be increased by applying a negative pressure of up to 50.8 cm Hg to the columns. Positive pressure may assist the withdrawal of the fractions. Subdued or infra-red light are desirable. According to a further embodiment, Figs. 6 to 8, fractions of the least dense sperm (Y sperm) are withdrawn from the top of column 60 through successively lower apertures 62 into vials 66 by opening valves 64, the central portion is emptied through a single valve 106 and fractions of the densest sperm (X sperm) are withdrawn into vials through successively lower apertures at the bottom of the column. The valves are sequentially operated by actuators through spring-biased cam followers 79 responsive to rotating cams 80 and 82 at the top and bottom of the column, respectively. The column is cooled by a flow of refrigerated air within a chamber 90 generated by impeller 88.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US814906A US3894529A (en) | 1969-04-10 | 1969-04-10 | Method and means for controlling the sex of mammalian offspring and product therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1309781A true GB1309781A (en) | 1973-03-14 |
Family
ID=25216316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1702670A Expired GB1309781A (en) | 1969-04-10 | 1970-04-09 | Method for fractionating sperm |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US3894529A (en) |
| JP (1) | JPS5536291B1 (en) |
| AT (1) | AT326402B (en) |
| BR (1) | BR7018158D0 (en) |
| CH (1) | CH563164A5 (en) |
| ES (1) | ES378413A1 (en) |
| GB (1) | GB1309781A (en) |
| TR (1) | TR16657A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2435948A1 (en) * | 1978-09-01 | 1980-04-11 | Skjervold Harald | IMPROVED ARTIFICIAL INSEMINATION METHOD |
| EP0041367A2 (en) * | 1980-06-02 | 1981-12-09 | The Regents Of The University Of California | Method and apparatus for fractionating cells |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3976197A (en) * | 1974-11-22 | 1976-08-24 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation method and apparatus for controlling the sex of mammalian offspring |
| USRE32350E (en) * | 1974-11-22 | 1987-02-10 | Bhairab C. Bhattacharya | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
| US4092229A (en) * | 1975-12-17 | 1978-05-30 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
| US4605558A (en) * | 1984-04-20 | 1986-08-12 | Wallace Shrimpton | Process for cell separation |
| US4999283A (en) * | 1986-01-10 | 1991-03-12 | University Of Kentucky Research Foundation | Method for x and y spermatozoa separation |
| US5346990A (en) * | 1987-04-08 | 1994-09-13 | Cytogam, Inc. | Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex |
| JP4323571B2 (en) | 1997-01-31 | 2009-09-02 | エックスワイ， インコーポレイテッド | Optical device |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US5908380A (en) * | 1998-01-12 | 1999-06-01 | Zavos; Panayiotis M. | Compartmentalized Zavos sperm swim-up column |
| BR9912539A (en) | 1998-07-30 | 2001-05-02 | Xy Inc | Equine system for non-surgical artificial insemination |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| EP2258172B1 (en) | 2000-05-09 | 2017-04-19 | Xy, Llc | Flow cytometer for diffentiating x-chromosome bearing and y-chromosome bearing populations of spermatozoa |
| CA2822983C (en) | 2000-11-29 | 2017-05-09 | Xy, Llc | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| JP4595067B2 (en) | 2002-08-01 | 2010-12-08 | エックスワイ，エルエルシー | Low-pressure sperm cell separation system |
| CN100570360C (en) | 2002-08-15 | 2009-12-16 | Xy公司 | A kind of flow cytometer and flow cytometry method |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| BR0300604A (en) * | 2003-02-17 | 2004-11-03 | Fundacao De Amparo A Pesquisa | Sex selection process of mammalian sperm and quality control method of frozen sexed semen doses |
| DK2305171T3 (en) | 2003-03-28 | 2022-03-21 | Inguran Llc | Apparatus and methods for providing sexed animal semen |
| ES2541121T3 (en) | 2003-05-15 | 2015-07-16 | Xy, Llc | Efficient classification of haploid cells by flow cytometry systems |
| US20050130115A1 (en) * | 2003-10-31 | 2005-06-16 | Abs Global,Inc. | Method for altering the gender ratio of offspring in mammals by manipulation of spermatozoa |
| US7892725B2 (en) | 2004-03-29 | 2011-02-22 | Inguran, Llc | Process for storing a sperm dispersion |
| AU2005266930B2 (en) * | 2004-07-22 | 2010-09-16 | Inguran, Llc | Process for enriching a population of sperm cells |
| ES2583985T3 (en) * | 2004-07-22 | 2016-09-23 | Inguran, Llc | Process to enrich a population of sperm cells |
| US7618770B2 (en) | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
| JP2010531142A (en) * | 2007-06-22 | 2010-09-24 | サークル バイオロジクス、 エルエルシー． | Liquid concentrator, autologous concentrated body fluid, and methods of use thereof |
| AU2008338530A1 (en) | 2007-12-14 | 2009-06-25 | Minitube Of America, Inc. | Gender-specific separation of sperm cells and embryos |
| WO2019043656A1 (en) | 2017-09-01 | 2019-03-07 | Genus Plc | Methods and systems for assessing and/or quantifying sex skewed sperm cell populations |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3185623A (en) * | 1960-05-31 | 1965-05-25 | Smith Fred | Preservation of animal semen |
-
1969
- 1969-04-10 US US814906A patent/US3894529A/en not_active Expired - Lifetime
-
1970
- 1970-04-09 ES ES378413A patent/ES378413A1/en not_active Expired
- 1970-04-09 AT AT327770A patent/AT326402B/en not_active IP Right Cessation
- 1970-04-09 GB GB1702670A patent/GB1309781A/en not_active Expired
- 1970-04-10 TR TR16657A patent/TR16657A/en unknown
- 1970-04-10 JP JP3023970A patent/JPS5536291B1/ja active Pending
- 1970-04-10 BR BR218158/70A patent/BR7018158D0/en unknown
- 1970-04-10 CH CH534270A patent/CH563164A5/xx not_active IP Right Cessation
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2435948A1 (en) * | 1978-09-01 | 1980-04-11 | Skjervold Harald | IMPROVED ARTIFICIAL INSEMINATION METHOD |
| EP0041367A2 (en) * | 1980-06-02 | 1981-12-09 | The Regents Of The University Of California | Method and apparatus for fractionating cells |
| EP0041367A3 (en) * | 1980-06-02 | 1982-08-18 | The Regents Of The University Of California | Method and apparatus for fractionating cells |
Also Published As
| Publication number | Publication date |
|---|---|
| BR7018158D0 (en) | 1973-04-12 |
| CH563164A5 (en) | 1975-06-30 |
| US3894529A (en) | 1975-07-15 |
| ES378413A1 (en) | 1973-08-16 |
| ATA327770A (en) | 1975-02-15 |
| JPS5536291B1 (en) | 1980-09-19 |
| TR16657A (en) | 1973-03-01 |
| AT326402B (en) | 1975-12-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PLNP | Patent lapsed through nonpayment of renewal fees |