GB1297581A - - Google Patents

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Publication number
GB1297581A
GB1297581A GB1297581DA GB1297581A GB 1297581 A GB1297581 A GB 1297581A GB 1297581D A GB1297581D A GB 1297581DA GB 1297581 A GB1297581 A GB 1297581A
Authority
GB
United Kingdom
Prior art keywords
culture medium
cells
carbon
carbon source
atcc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of GB1297581A publication Critical patent/GB1297581A/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic
    • C12N1/30—Processes using, or culture media containing, hydrocarbons aliphatic having five or less carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1297581 Cultivating mycobacteria NIPPON OIL CO Ltd 16 Sept 1970 [16 Sept 1969] 44247/70 Heading C6F A method of preparing protein-containing cells involves inoculating a culture medium containing a nitrogen source and a carbon source which comprises carbon dioxide, a saturated hydrocarbon having 1 to 5 carbon atoms or an n-paraffin having 11 to 18 carbon atoms, with Mycobaterium cuneatum ATCC 21498 or Mycobacterium petroleophilum ATCC 21497, culturing the microorganism cells under aerobic conditions, separating the cells from the culture medium and (optionally) freeze-drying or spray-drying them. The carbon source may be a gaseous mixture of carbon dioxide with hydrogen, liquefied petroleum gas consisting mainly of propane and butane, byproduct gases consisting predominantly of hydrogen and methane discharged from naphtha cracking apparatus, fuel gases discharged from petroleum refining apparatus, kerosene, light oil or heavy oil. When using liquid hydrocarbons as the carbon source a small amount of surface active agent may be included in the culture medium and the separated cells after cultivation may be treated with surface active agent and extracted with an organic solvent such as hexane, ethyl ether, acetone, liquefied petroleum gas (butane, propane) or a chlorinating solvent to remove the remaining hydrocarbon sufficiently.
GB1297581D 1969-09-16 1970-09-16 Expired GB1297581A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7299269 1969-09-16

Publications (1)

Publication Number Publication Date
GB1297581A true GB1297581A (en) 1972-11-22

Family

ID=13505394

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1297581D Expired GB1297581A (en) 1969-09-16 1970-09-16

Country Status (2)

Country Link
FR (1) FR2062188A5 (en)
GB (1) GB1297581A (en)

Also Published As

Publication number Publication date
FR2062188A5 (en) 1971-06-25

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee