GB1278019A - A process for purifying microorganisms grown on hydrocarbon substrates - Google Patents

A process for purifying microorganisms grown on hydrocarbon substrates

Info

Publication number
GB1278019A
GB1278019A GB4428170A GB4428170A GB1278019A GB 1278019 A GB1278019 A GB 1278019A GB 4428170 A GB4428170 A GB 4428170A GB 4428170 A GB4428170 A GB 4428170A GB 1278019 A GB1278019 A GB 1278019A
Authority
GB
United Kingdom
Prior art keywords
hydrocarbon
micro
hydrocarbons
organisms
hydrocarbon solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4428170A
Inventor
C Decerle
A Deschamps
S Frankowiak
C Gatellier
G Glikmans
P Renault
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IFP Energies Nouvelles IFPEN
Original Assignee
IFP Energies Nouvelles IFPEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IFP Energies Nouvelles IFPEN filed Critical IFP Energies Nouvelles IFPEN
Publication of GB1278019A publication Critical patent/GB1278019A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1278019 Yeast &c INSTITUT FRANCAIS DU PETROLES DES CARBURANTS ET LUBRIFIANTS 16 Sept 1970 [18 Sept 1969] 44281/70 Heading C6F Micro-organisms from a hydrocarbon fermentation contaminated with hydrocarbon are contacted simultaneously with a surfactant and with a hydrocarbon solvent selected from (i) aliphatic, cycloaliphatic and aromatic hydrocarbons of boiling points between 30 and 200‹C, at standard pressure and (ii) straight-chain aliphatic C 9 -C 25 hydrocarbons, and the resulting purified microorganisms are separated from the surfactant and from the hydrocarbon solvent. Surfactants: non-ionic condensates of ethylene oxide (or propylene oxide) groups with lauryl alcohol, stearic acid, nonyl phenol, resin, dodecyl mercaptan, octylamine, lauryl mono-ethanolamide and tallow sugar glyceride. Solvents: (i) pentane, hexane, heptane, iso-octane, nonane, cyclohexane, methyl cyclohexane, benzene and (ii) nonane, n-decane, n-dodecane and n-hexadecane. The micro-organism is dispersed in an aqueous phase at a concentration of 5 to 150g (dry weight) per litre of aqueous phase before being contacted with the surfactant and hydrocarbon solvent. Residual hydrocarbon solvent may be taken up from the purified micro-organisms in a stream of inert gas or super-heated steam or by boiling. When the straight-chain aliphatic Ca-C 25 hydrocarbons are used as solvent, traces of these and of residual hydrocarbons from the fermentation can be at least partly eliminated from the micro-organisms by further fermentation in an aqueous medium without added hydrocarbon. Hydrocarbon solvent separated from the treated micro-organisms can be passed to the hydrocarbon fermentation. Examples are directed to the treatment of cells of Candida lipolytica after cultivation upon hydrocarbons, with hexane containing one of the abovementioned surfactants.
GB4428170A 1969-09-18 1970-09-16 A process for purifying microorganisms grown on hydrocarbon substrates Expired GB1278019A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR6931888A FR2058634A5 (en) 1969-09-18 1969-09-18

Publications (1)

Publication Number Publication Date
GB1278019A true GB1278019A (en) 1972-06-14

Family

ID=9040290

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4428170A Expired GB1278019A (en) 1969-09-18 1970-09-16 A process for purifying microorganisms grown on hydrocarbon substrates

Country Status (9)

Country Link
JP (1) JPS4834236B1 (en)
BE (1) BE756254A (en)
DE (1) DE2045991A1 (en)
EG (1) EG10263A (en)
FR (1) FR2058634A5 (en)
GB (1) GB1278019A (en)
NL (1) NL7013437A (en)
SE (1) SE377471B (en)
SU (1) SU404276A3 (en)

Also Published As

Publication number Publication date
DE2045991A1 (en) 1971-03-25
JPS4834236B1 (en) 1973-10-19
BE756254A (en) 1971-03-17
NL7013437A (en) 1971-03-22
FR2058634A5 (en) 1971-05-28
SU404276A3 (en) 1973-10-26
EG10263A (en) 1976-03-31
SE377471B (en) 1975-07-07

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees