GB1194207A - Spectrophotometric Determination of Creatine Phosphokinase - Google Patents

Spectrophotometric Determination of Creatine Phosphokinase

Info

Publication number
GB1194207A
GB1194207A GB3283967A GB3283967A GB1194207A GB 1194207 A GB1194207 A GB 1194207A GB 3283967 A GB3283967 A GB 3283967A GB 3283967 A GB3283967 A GB 3283967A GB 1194207 A GB1194207 A GB 1194207A
Authority
GB
United Kingdom
Prior art keywords
test solution
cpk
creatine
spectrophotometric
taking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3283967A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Chemical Co
Original Assignee
Sigma Chemical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Chemical Co filed Critical Sigma Chemical Co
Publication of GB1194207A publication Critical patent/GB1194207A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,194,207. Determining creative phosphokinase. SIGMA CHEMICAL CO. 17 July, 1967 [26 Sept., 1966], No. 32839/67. Heading D1X. Creatine phosphokinase (CPK) is determined by reacting ATP with creatine in a test solution containing an unknown quantity of CPK to form ADP, by a method comprising adding to the test solution a sulfhydryl compound, a buffer to maintain a pH of about 9, phospho (enol) pyruvate, pyruvate kinase, lactic dehydrogenase and DPNH, taking a first spectrophotometric measurement about 5 minutes after all the reactants have been added to the test solution, taking a second spectrophotometric measurament at the same wavelength at a known interval of time after the first measurement, and determining the CPK in the test solution from the difference between the first and second measurements. The sulfhydryl compound may be glutathione in its reduced form. The reactions used in the test are Reference has been directed by the Comptroller to Specification 1,153,273.
GB3283967A 1966-09-26 1967-07-17 Spectrophotometric Determination of Creatine Phosphokinase Expired GB1194207A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58171166A 1966-09-26 1966-09-26

Publications (1)

Publication Number Publication Date
GB1194207A true GB1194207A (en) 1970-06-10

Family

ID=24326269

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3283967A Expired GB1194207A (en) 1966-09-26 1967-07-17 Spectrophotometric Determination of Creatine Phosphokinase

Country Status (6)

Country Link
AT (1) AT285538B (en)
BE (1) BE703709A (en)
CH (1) CH504686A (en)
GB (1) GB1194207A (en)
NL (1) NL6712441A (en)
NO (1) NO122809B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2426263A1 (en) * 1978-07-17 1979-12-14 Beckman Instruments Inc
CN110699423A (en) * 2019-10-22 2020-01-17 浙江大学 Reagent, kit and method for detecting concentration of oxalic acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2426263A1 (en) * 1978-07-17 1979-12-14 Beckman Instruments Inc
CN110699423A (en) * 2019-10-22 2020-01-17 浙江大学 Reagent, kit and method for detecting concentration of oxalic acid

Also Published As

Publication number Publication date
NO122809B (en) 1971-08-16
BE703709A (en) 1968-03-11
NL6712441A (en) 1968-03-27
CH504686A (en) 1971-03-15
AT285538B (en) 1970-10-27

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Legal Events

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PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee