GB1152386A - Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation - Google Patents

Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation

Info

Publication number
GB1152386A
GB1152386A GB9766/67A GB976667A GB1152386A GB 1152386 A GB1152386 A GB 1152386A GB 9766/67 A GB9766/67 A GB 9766/67A GB 976667 A GB976667 A GB 976667A GB 1152386 A GB1152386 A GB 1152386A
Authority
GB
United Kingdom
Prior art keywords
antibiotic
ethyl acetate
solvent
antibiotics
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB9766/67A
Inventor
Denise Mancy
Leon Ninet
Jean Preud Homme
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhone Poulenc SA
Original Assignee
Rhone Poulenc SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc SA filed Critical Rhone Poulenc SA
Publication of GB1152386A publication Critical patent/GB1152386A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G11/00—Antibiotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/55—Streptomyces hygroscopicus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/56—Streptomyces lavendulae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

1,152,386. Antibiotics 16,511 R.P. and 18,051 R.P. RHONE - POULENC S.A. 1 March, 1967 [2 March, 1966; 13 Jan., 1967], No. 9766/67. Heading C2A. The invention relates to an antibiotic 16,511 R.P., which is a yellow amorphous powder of m. pt. 114‹ C. and which is an acid forming salts with bases, e.g. alkali metal salts; it is soluble in methanol and ethanol, methylene chloride, chloroform, ethyl acetate, dimethylformamide, dimethylsulphoxide and acetone, less so in benzene and diethyl ether and practically insoluble in water, cyclohexane and hexane; it has the elementary analysis C= = 65À8%, H=7À8%, O=22À7%, N=3À55%; its ultraviolet spectrum (Fig. 1) shows absorption max. at 232 nm. (E<SP>1%</SP> 1 cm =682) and 350 nm max. at 232 nm. (E<SP>1%</SP> 1cm = 682) and 350 nm (E<SP>1%</SP> 1 cm. = 375) and minimum at 260 nm (E<SP>1%</SP> 1 cm. =130); its infra-red spectrum is shown in Fig. 3; [α] D <SP>22</SP> = - 121‹Œ2‹ (C=1À0); it contains components A (Rf value=0À1), B (Rf=0À2) also known as 18,051 R.P., C (Rf= 0À35) and D (Rf=0À60). Rf value is determined by thin layer chromatography using a Kieselgel H support and ethyl acetate as solvent. Antibiotic 18,051 R.P. is a yellow amorphous powder of m. pt. 112‹ C.; having the elementary analysis C=64À7%, H=8À2%, O=23À75%, N=3À35%; U.V. as in Fig. 6 (not shown); I.R. as in Fig. 8 (not shown), and [α]<SP>22</SP> D = - 116À5‹Œ 5À5‹ (C=0À73). Antibiotic 16,511 R.P. is produced by aerobically cultivating Streptomyces hygroscopicus var. tepidalitus NRRL 3237, Streptomyces lavendulae NRRL 3277, Streptomyces lavendulae NRRL 3278, or a 16,511 R.P.- producing mutant thereof. 16,511 R.P. may be isolated from the fermentation broth by (a) solvent extraction of the whole or clarified broth, (b) adsorption or strongly anionic exchange resins from a clarified broth, followed by elution with aqueous alcohol containing an electrolyte, e.g. sodium chloride. Purification may be by (a) precipitation from solution by a non-solvent such as hexane, (b) chromatography on alumina, silica gel or florisil, (c) treatment with carbon. 18,051 R.P. is separated from 16,511 R.P. by (A) chromatography on Kieselgel buffered at pH 4À0 with M/15 phosphate buffer, the chromatogram being developed with ethyl acetate; (B) selective extraction with a methylene chloride-cyclohexane M/15 phosphate buffer at pH 9À0 (80: 20: 100 by volume). The antibiotics are effective against Gram positive micro-organisms. Pharmaceutical compositions, e.g. injections, comprise at least one of 16,511 R.P. and 18,051 R.P. or a non-toxic salt thereof and a carrier.
GB9766/67A 1966-03-02 1967-03-01 Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation Expired GB1152386A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR51696A FR1516739A (en) 1966-03-02 1966-03-02 New antibiotics and their methods of preparation by culture of streptomyces hygroscopicus
FR91136 1967-01-13

Publications (1)

Publication Number Publication Date
GB1152386A true GB1152386A (en) 1969-05-14

Family

ID=26168950

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9766/67A Expired GB1152386A (en) 1966-03-02 1967-03-01 Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation

Country Status (9)

Country Link
AT (1) AT270066B (en)
BE (1) BE694872A (en)
CH (1) CH472494A (en)
ES (1) ES337456A1 (en)
FR (2) FR1516739A (en)
GB (1) GB1152386A (en)
GR (1) GR32437B (en)
LU (1) LU53088A1 (en)
NL (1) NL6702707A (en)

Also Published As

Publication number Publication date
ES337456A1 (en) 1968-03-01
GR32437B (en) 1967-07-04
CH472494A (en) 1969-05-15
FR1516739A (en) 1968-02-05
BE694872A (en) 1967-09-01
AT270066B (en) 1969-04-10
LU53088A1 (en) 1967-09-01
NL6702707A (en) 1967-09-04
FR1577210A (en) 1969-08-08

Similar Documents

Publication Publication Date Title
IE36434L (en) Gentamicin antibiotics.
GB1058686A (en) The antibiotic rubradirin
IE33857L (en) Daunorubicin
GB1357538A (en) New antibiotic
GB1152386A (en) Antibiotics 16,511 R.P. and 18,051 R.P. and their preparation
GB1303842A (en)
GB1350271A (en) Antibiotic and a process for its preparation
IE41640L (en) Production of antibiotic substances
IE37892L (en) Antibiotic a.2315.
ES252350A1 (en) The new organic compound tylosin, its hydrolysis product desmycosin and methods for preparing same
GB1292010A (en) A new antibiotic, its production and its use
GB1131079A (en) The antibiotic armentomycin
GB1212741A (en) A method for producing an antibiotic b-2847
GB1226665A (en)
GB1159970A (en) The Antibiotic Steffisburgensimycin
GB1356723A (en) Antibiotic sf-1293 substance and its production and uses
ES341402A1 (en) Antibiotics of agricultural fungicides,polyoxins d,e,f,g and h
GB1387080A (en) Antibiotics and the preparation thereof
GB1224379A (en) New antibiotic nk-1003 and processes for the production of the same
ES350779A1 (en) Antibiotic 19,402 r.p.
GB1488629A (en) Antibiotic
GB1113501A (en) Antibiotic ra-6950b and method of production
ES356123A1 (en) Preparation of the antibiotic rifamycin sv
GB1387769A (en) Butenoic acid derivatives and a process for the manufacture thereof
GB1284949A (en) Novel antibiotics and methods of preparing same

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PLNP Patent lapsed through nonpayment of renewal fees