GB1108770A - Process and composition for the enzymatic determination of glucose - Google Patents

Process and composition for the enzymatic determination of glucose

Info

Publication number
GB1108770A
GB1108770A GB36385/66A GB3638566A GB1108770A GB 1108770 A GB1108770 A GB 1108770A GB 36385/66 A GB36385/66 A GB 36385/66A GB 3638566 A GB3638566 A GB 3638566A GB 1108770 A GB1108770 A GB 1108770A
Authority
GB
United Kingdom
Prior art keywords
phosphate
glucose
transferase
acylate
donor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB36385/66A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1108770A publication Critical patent/GB1108770A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/14—Beverages
    • G01N33/143—Beverages containing sugar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/823—Acetobacter

Abstract

Glucose is determined by a sequence of reactions comprising converting glucose into glucose-6-phosphate by means of a phosphate donor and a transferase, converting the glucose-6-phosphate into 6-phosphogluconic acid by means of triphosphopyridine-nucleotide in the presence of glucose-6-phosphoric acid dehydrogenase, reduced triphosphopyridine-nucleotide being formed at the same time, and then determining the latter spectrophotometrically: characterised in that phosphate donor is a compound of the general formula:- <FORM:1108770/B1-B2/1> in which R is an organic radical, said radical not possessing a negative charge in the immediate neighbourhood of the phospate group, and wherein the transferase is acylphosphate: glucose p transferase. Specified phosphate donors are the following phosphates:-benzoyl, nicotoyl, acetyl, propionyl, N-benzoyl, glycoyl, carbonyl, glutaroyl, succinyl, and 3-phosphoglyceric acid-1-phosphate. The phosphate donor may be synthesized immediately before glucose determination by reacting acylate ions and an acylate-phosphokinase (e.g. acetyl phosphate from an acetate, adenosine triphosphate by means of acetate - kinase, and carbamyl phosphate from carbamic acid and ATP by means of carbamatephosphokinase.) Examples describe the analysis of various fruit juices and blood for glucose. Fructose, if present, does not interfere. In one Example, the acyl phosphate used is derived from a glycocollacetate buffer of pH 9.0, and magnesium chloride is also be present. The sample to be determined is mixed with all the reactant except the acyl phosphate-glucose transferase, and the latter is then added to start the reaction. Glucose is determined spectrophotometrically after some minutes. The invention also comprises a composition comprising the above defined phosphate donor, acyl phosphate: glucose transferase, glucose-6-phosphate-dehydrogenase and triphosphopyridine nucleotide: or a similar composition in which the phosphate donor is replaced by adenosine triphosphate, acylate ions and an acylate-phosphokinase.
GB36385/66A 1951-01-28 1966-08-15 Process and composition for the enzymatic determination of glucose Expired GB1108770A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19511598067 DE1598067A1 (en) 1951-01-28 1951-01-28 Methods and diagnostic agents for the enzymatic determination of glucose
DEB0083297 1965-08-17

Publications (1)

Publication Number Publication Date
GB1108770A true GB1108770A (en) 1968-04-03

Family

ID=25753451

Family Applications (1)

Application Number Title Priority Date Filing Date
GB36385/66A Expired GB1108770A (en) 1951-01-28 1966-08-15 Process and composition for the enzymatic determination of glucose

Country Status (9)

Country Link
US (1) US3509025A (en)
JP (1) JPS503674B1 (en)
BE (1) BE685538A (en)
CH (1) CH488186A (en)
DE (1) DE1598067A1 (en)
GB (1) GB1108770A (en)
NL (1) NL6611550A (en)
NO (1) NO117818B (en)
SE (1) SE337492B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56169598A (en) * 1980-05-26 1981-12-26 Mitsubishi Petrochem Co Ltd Measuring composition
JPS59151899A (en) * 1983-02-16 1984-08-30 Unitika Ltd Determination composition
JPS62232555A (en) * 1986-04-02 1987-10-13 Unitika Ltd Oxygen sensor
US4935346A (en) * 1986-08-13 1990-06-19 Lifescan, Inc. Minimum procedure system for the determination of analytes
US6458326B1 (en) 1999-11-24 2002-10-01 Home Diagnostics, Inc. Protective test strip platform
TWI275795B (en) * 2001-02-14 2007-03-11 Sysmex Corp Novel assay method
US6562625B2 (en) 2001-02-28 2003-05-13 Home Diagnostics, Inc. Distinguishing test types through spectral analysis
US6525330B2 (en) 2001-02-28 2003-02-25 Home Diagnostics, Inc. Method of strip insertion detection
US6541266B2 (en) 2001-02-28 2003-04-01 Home Diagnostics, Inc. Method for determining concentration of an analyte in a test strip

Also Published As

Publication number Publication date
NL6611550A (en) 1967-02-20
CH488186A (en) 1970-03-31
DE1598067C3 (en) 1974-04-18
US3509025A (en) 1970-04-28
BE685538A (en) 1967-02-16
DE1598067A1 (en) 1970-03-26
NO117818B (en) 1969-09-29
SE337492B (en) 1971-08-09
DE1598067B2 (en) 1973-09-27
JPS503674B1 (en) 1975-02-07

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