GB1091045A - Production of 6-demethyltetracycline - Google Patents
Production of 6-demethyltetracyclineInfo
- Publication number
- GB1091045A GB1091045A GB44991/64A GB4499164A GB1091045A GB 1091045 A GB1091045 A GB 1091045A GB 44991/64 A GB44991/64 A GB 44991/64A GB 4499164 A GB4499164 A GB 4499164A GB 1091045 A GB1091045 A GB 1091045A
- Authority
- GB
- United Kingdom
- Prior art keywords
- deficient
- methionine
- aureofaciens
- gene
- strains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/65—Tetracyclines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P29/00—Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
- C12R2001/485—Streptomyces aureofaciens
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
6-Dimethyl tetracycline antibiotic producing gene-recombinants of a species of the genus Streptomyces are obtained by subjecting two different strains of a tetracycline antibiotic producing species of Streptomyces, one of which strains is deficient in its methionine biosynthesis, to gene-recombination in the presence of a folic acid antagonist and selecting and isolating a 6-dimethyl tetracycline-producing strain from the resulting culture. The strain or strains other than the methionine deficient strains may exhibit normal biosynthetic processes or be deficient in a biosynthetic substance other than methionine. The methionine deficient strain may exhibit an additional biosynthetic deficiency. The folic acid antagonist includes 4-amino-pteroyl-glutamic acid, 4-amino-10-methyl-pteroyl glutamic acid and sulphonamides of formula:- <FORM:1091045/C6-C7/1> where R is a substituted or unsubstituted guanidine, thiazole, pyrimidine or pyrazino radical. The gene-recombination may be effected in generally known manner in a "minimal medium" to which is added the substances in which the strains are deficient in their biosynthesis. In examples, gene-recombinants occur when the two mutants are as follows:- (a) Streptomyces aureofaciens methionine deficient with S. aureofaciens arginine and cysteine deficient. (b) S. aureofaciens methionine and amino nitrogen deficient with S. aureofaciens methionine and asparagine deficient. (c) S. aureofaciens methione and asparagine deficient with S. aureofaciens arginine and cysteine deficient.ALSO:6 - Demethyltetracycline - antibiotic - producing gene-recombinants of a species of the genus Streptomyces are obtained by subjecting two different strains of a tetracycline-antibiotic-producing species of Streptomyces, one of which strains is deficient in its methionine biosynthesis to gene recombination in the presence of a folic acid antagonist, and selecting and isolating a 6-demethyl-tetracycline-producing strain from the resulting culture. The strain or strains other than the methionine-deficient strain may exhibit normal biosynthetic processes or be deficient in a biosynthetic substance other than methionine. The methionine-deficient strain may exhibit an additional biosynthetic deficiency. The folic acid antagonist includes 4-amino-pteroylglutamic acid, 4-amino - 10 - methyl - pteroylglutamic acid and sulphonamides of formula <FORM:1091045/C2/1> wherein R is a substituted or unsubstituted guanidine, thiazole, pyrimidine or pyrazino radical. The gene-recombination may be effected in generally known manner in a "minimal medium" to which is added the substances in which the strains are deficient in their biosynthesis. In examples, gene-recombinants occur when the two mutants are as follows: (a) Streptomyces aureofaciens methionine-deficient, with S. aureofaciens arginine- and cysteine-deficient, (b) S. aureofaciens methionine-and amino-nitrogen-deficient with S. aureofaciens cysteine- and asparagine-deficient, (e) S. aureofaciens methionine- and asparagine-deficient with S. aureofaciens arginine- and cysteine-deficient. A 6-demethyltetracycline antibiotic is produced by culturing in a conventional manner a gene-recombinant as above or a descendant or mutant thereof. Chloride ions, bromide ions or chlorination or bromination inhibitors may be present to produce predominantly the appropriate chlorotetracycline, bromotetracycline or tetracycline. A methylation inhibitor may be added gradually in accordance with Specification 1,091,046.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HUCI000479 | 1963-11-05 | ||
HUCI000480 | 1963-11-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1091045A true GB1091045A (en) | 1967-11-15 |
Family
ID=26318372
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB44991/64A Expired GB1091045A (en) | 1963-11-05 | 1964-11-04 | Production of 6-demethyltetracycline |
GB20660/67A Expired GB1091046A (en) | 1963-11-05 | 1964-11-04 | Production of 6-demethyltetracyclines |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB20660/67A Expired GB1091046A (en) | 1963-11-05 | 1964-11-04 | Production of 6-demethyltetracyclines |
Country Status (4)
Country | Link |
---|---|
AT (1) | AT264710B (en) |
DE (1) | DE1467833C3 (en) |
DK (1) | DK129243B (en) |
GB (2) | GB1091045A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110699314B (en) * | 2019-10-22 | 2022-12-23 | 河北圣雪大成制药有限责任公司 | Method for producing 6-demethyltetracycline by fermentation |
-
1964
- 1964-11-03 AT AT930464A patent/AT264710B/en active
- 1964-11-04 GB GB44991/64A patent/GB1091045A/en not_active Expired
- 1964-11-04 GB GB20660/67A patent/GB1091046A/en not_active Expired
- 1964-11-04 DE DE1467833A patent/DE1467833C3/en not_active Expired
- 1964-11-04 DK DK543564AA patent/DK129243B/en unknown
Also Published As
Publication number | Publication date |
---|---|
AT264710B (en) | 1968-09-10 |
GB1091046A (en) | 1967-11-15 |
DE1467833A1 (en) | 1968-11-28 |
DE1467833B2 (en) | 1973-10-04 |
DK129243C (en) | 1975-02-03 |
DK129243B (en) | 1974-09-16 |
DE1467833C3 (en) | 1974-05-02 |
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