GB1010919A - - Google Patents

Info

Publication number
GB1010919A
GB1010919A GB3896262A GB3896262A GB1010919A GB 1010919 A GB1010919 A GB 1010919A GB 3896262 A GB3896262 A GB 3896262A GB 3896262 A GB3896262 A GB 3896262A GB 1010919 A GB1010919 A GB 1010919A
Authority
GB
United Kingdom
Prior art keywords
viruses
column
virus
suspension
phosphate buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3896262A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of GB1010919A publication Critical patent/GB1010919A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32611—Poliovirus
    • C12N2770/32651—Methods of production or purification of viral material

Abstract

Aggregations of viruses in suspension are separated into single viruses and also purified by subjecting an aqueous suspension of the virus to chromatography at a speed of flow of 0.001 to 0.400 cc per minute per cm2 in a calcium phosphate adsorption column, which has been filled at a pressure of 500-600 m.m. of Hg with a suspension of baHP04 2H2O in a phosphate buffer having a pH value of 6.5 to 7.5 and a molar concentration of about 0.001 m, the ratio of the diameter of the column to its length being 1 : 10 to 1 : 20 and the concentration of the virus suspension being about 4000-6000 gamma N. per cc, washing the column with a phosphate buffer having a pH of 6.5 to 7.5 and a molar concentration about 0.001 m. and electing the adsorbed viruses from the column at a speed of flow of 0.001-0.400 cc per minute per cm2 with a phosphate buffer having a pH value of 6.5 to 7.5 and a molar concentration of 0.1 to 1.0 m. The virus may be further purified by treatment with an anion exchange cellulose derivative. Typical viruses are poliomyelitis and influenza virus.
GB3896262A 1961-10-14 1962-10-15 Expired GB1010919A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEB64383A DE1182391B (en) 1961-10-14 1961-10-14 Process for the deaggregation and purification of viruses

Publications (1)

Publication Number Publication Date
GB1010919A true GB1010919A (en) 1965-11-24

Family

ID=6974367

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3896262A Expired GB1010919A (en) 1961-10-14 1962-10-15

Country Status (3)

Country Link
DE (1) DE1182391B (en)
GB (1) GB1010919A (en)
SE (1) SE303174B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1814982B1 (en) 2004-10-22 2015-12-23 Oncolytics Biotech Inc. Improved viral purification methods

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1814982B1 (en) 2004-10-22 2015-12-23 Oncolytics Biotech Inc. Improved viral purification methods

Also Published As

Publication number Publication date
DE1182391B (en) 1964-11-26
SE303174B (en) 1968-08-19

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