FR3100819B1 - Production et fonctionnalisation de nanoparticules dérivées du phage T5 et utilisations thérapeutiques - Google Patents
Production et fonctionnalisation de nanoparticules dérivées du phage T5 et utilisations thérapeutiques Download PDFInfo
- Publication number
- FR3100819B1 FR3100819B1 FR1910305A FR1910305A FR3100819B1 FR 3100819 B1 FR3100819 B1 FR 3100819B1 FR 1910305 A FR1910305 A FR 1910305A FR 1910305 A FR1910305 A FR 1910305A FR 3100819 B1 FR3100819 B1 FR 3100819B1
- Authority
- FR
- France
- Prior art keywords
- phage
- functional fragment
- fragment
- relates
- capsid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002105 nanoparticle Substances 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 230000001225 therapeutic effect Effects 0.000 title abstract 2
- 241000701988 Escherichia virus T5 Species 0.000 title 1
- 238000007306 functionalization reaction Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 9
- 210000000234 capsid Anatomy 0.000 abstract 6
- 102000037865 fusion proteins Human genes 0.000 abstract 4
- 108020001507 fusion proteins Proteins 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 239000005556 hormone Substances 0.000 abstract 2
- 229940088597 hormone Drugs 0.000 abstract 2
- 230000008685 targeting Effects 0.000 abstract 2
- 239000003053 toxin Substances 0.000 abstract 2
- 231100000765 toxin Toxicity 0.000 abstract 2
- 101710087971 Decoration protein Proteins 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/385—Haptens or antigens, bound to carriers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55566—Emulsions, e.g. Freund's adjuvant, MF59
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
- A61K2039/572—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 cytotoxic response
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10023—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10041—Use of virus, viral particle or viral elements as a vector
- C12N2795/10042—Use of virus, viral particle or viral elements as a vector virus or viral particle as vehicle, e.g. encapsulating small organic molecule
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne des capsides de phage T5 dépourvue d’ADN génomique dudit phage et exposant, à sa surface, au moins une protéine de fusion d’intérêt. L’invention concerne en particulier une capside de phage T5 dépourvue d’ADN génomique dudit phage et exposant, à sa surface, au moins une protéine de fusion, ladite protéine de fusion comprenant : - au moins un fragment de peptide ou de protéine ayant au moins 80% d’identité avec un fragment d’une protéine de décoration pb10 ; et - au moins un fragment fonctionnel d’un antigène, ou au moins un fragment fonctionnel d’une toxine, ou au moins un fragment de récepteur, ou au moins un fragment fonctionnel d’un signal d’adressage ou de ciblage ou de transport, ou au moins un fragment fonctionnel d’une enzyme, ou au moins un fragment fonctionnel d’une hormone, ou au moins un fragment fonctionnel d’un anticorps, ou au moins un antigène, ou au moins une toxine, ou au moins un récepteur, ou au moins un signal d’adressage ou de ciblage ou de transport, ou au moins une enzyme, ou au moins une hormone, ou au moins un anticorps, ou une combinaison quelconque de ceux-ci. La présente invention porte également sur les méthodes de production d’une telle capside et sur des vecteurs permettant leur production. L’invention concerne en outre les protéines de fusion d’intérêt exposées sur la capside et les acides nucléiques les codant. L’invention concerne également des nanoparticules comprenant ces capsides fonctionnalisées, des compositions pharmaceutiques comprenant ces nanoparticules et/ou ces capsides fonctionnalisées, ainsi que leurs utilisations thérapeutiques, notamment comme médicament et/ou comme vaccin.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1910305A FR3100819B1 (fr) | 2019-09-18 | 2019-09-18 | Production et fonctionnalisation de nanoparticules dérivées du phage T5 et utilisations thérapeutiques |
CA3154856A CA3154856A1 (fr) | 2019-09-18 | 2020-09-18 | Production et fonctionnalisation de nanoparticules derivees du phage t5 et utilisations therapeutiques |
JP2022518200A JP2022549257A (ja) | 2019-09-18 | 2020-09-18 | ファージt5由来ナノ粒子の作製及び機能化並びに治療用途 |
EP20785815.0A EP4031668A1 (fr) | 2019-09-18 | 2020-09-18 | Production et fonctionnalisation de nanoparticules dérivées du phage t5 et utilisations thérapeutiques |
US17/761,403 US20230190925A1 (en) | 2019-09-18 | 2020-09-18 | Production and functionalization of nanoparticles derived from phage t5 and therapeutic uses |
PCT/FR2020/051628 WO2021053309A1 (fr) | 2019-09-18 | 2020-09-18 | Production et fonctionnalisation de nanoparticules dérivées du phage t5 et utilisations thérapeutiques |
CN202080079575.0A CN114729375A (zh) | 2019-09-18 | 2020-09-18 | 源自噬菌体t5的纳米颗粒的生产和功能化以及治疗用途 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1910305 | 2019-09-18 | ||
FR1910305A FR3100819B1 (fr) | 2019-09-18 | 2019-09-18 | Production et fonctionnalisation de nanoparticules dérivées du phage T5 et utilisations thérapeutiques |
Publications (2)
Publication Number | Publication Date |
---|---|
FR3100819A1 FR3100819A1 (fr) | 2021-03-19 |
FR3100819B1 true FR3100819B1 (fr) | 2024-04-19 |
Family
ID=71094391
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR1910305A Active FR3100819B1 (fr) | 2019-09-18 | 2019-09-18 | Production et fonctionnalisation de nanoparticules dérivées du phage T5 et utilisations thérapeutiques |
Country Status (7)
Country | Link |
---|---|
US (1) | US20230190925A1 (fr) |
EP (1) | EP4031668A1 (fr) |
JP (1) | JP2022549257A (fr) |
CN (1) | CN114729375A (fr) |
CA (1) | CA3154856A1 (fr) |
FR (1) | FR3100819B1 (fr) |
WO (1) | WO2021053309A1 (fr) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE359295T1 (de) * | 2002-06-11 | 2007-05-15 | Glaxosmithkline Biolog Sa | Immunogene zusammensetzungen |
-
2019
- 2019-09-18 FR FR1910305A patent/FR3100819B1/fr active Active
-
2020
- 2020-09-18 JP JP2022518200A patent/JP2022549257A/ja active Pending
- 2020-09-18 US US17/761,403 patent/US20230190925A1/en active Pending
- 2020-09-18 CA CA3154856A patent/CA3154856A1/fr active Pending
- 2020-09-18 CN CN202080079575.0A patent/CN114729375A/zh active Pending
- 2020-09-18 WO PCT/FR2020/051628 patent/WO2021053309A1/fr unknown
- 2020-09-18 EP EP20785815.0A patent/EP4031668A1/fr active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2021053309A1 (fr) | 2021-03-25 |
CA3154856A1 (fr) | 2021-03-25 |
JP2022549257A (ja) | 2022-11-24 |
CN114729375A (zh) | 2022-07-08 |
EP4031668A1 (fr) | 2022-07-27 |
FR3100819A1 (fr) | 2021-03-19 |
US20230190925A1 (en) | 2023-06-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PLFP | Fee payment |
Year of fee payment: 2 |
|
PLSC | Publication of the preliminary search report |
Effective date: 20210319 |
|
PLFP | Fee payment |
Year of fee payment: 3 |
|
TQ | Partial transmission of property |
Owner name: UNIVERSITE PARIS-SACLAY, FR Effective date: 20211221 Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (, FR Effective date: 20211221 |
|
PLFP | Fee payment |
Year of fee payment: 4 |
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PLFP | Fee payment |
Year of fee payment: 5 |