FR3075817B1 - MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP - Google Patents

MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP Download PDF

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Publication number
FR3075817B1
FR3075817B1 FR1762895A FR1762895A FR3075817B1 FR 3075817 B1 FR3075817 B1 FR 3075817B1 FR 1762895 A FR1762895 A FR 1762895A FR 1762895 A FR1762895 A FR 1762895A FR 3075817 B1 FR3075817 B1 FR 3075817B1
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FR
France
Prior art keywords
well
micro
fluidic chip
chip
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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FR1762895A
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French (fr)
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FR3075817A1 (en
Inventor
Sarah Milgram
Damien Fleury
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Nanobiose
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Nanobiose
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Publication date
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Priority to FR1762895A priority Critical patent/FR3075817B1/en
Priority to US16/956,632 priority patent/US20200406263A1/en
Priority to PCT/FR2018/053524 priority patent/WO2019122788A1/en
Priority to EP18845401.1A priority patent/EP3728556A1/en
Publication of FR3075817A1 publication Critical patent/FR3075817A1/en
Application granted granted Critical
Publication of FR3075817B1 publication Critical patent/FR3075817B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La puce micro-fluidique (1) comporte : • au moins un canal d'entrée (4) connecté à au moins un puits (2), chaque puits (2) étant associé à un canal de sortie (5), • au moins une chambre d'analyse (3a, 3b) connectée à au moins un puits (2). • une surface d'analyse (6) comportant des éléments de captation (6a) capturant des biomarqueurs présents dans le liquide issu du puits (2) et représentatifs de la réponse cellulaire d'un échantillon biologique contenu dans le puits (2). Un flux de liquide s'écoule depuis le puits (2) vers la surface d'analyse (6) comportant les éléments de captation (6a). Le flux de liquide est compris entre 0,1µL/min et 2 mL/min et s'écoule de façon laminaire dans la chambre d'analyse (3a, 3b). La puce (1) fait partie d'un laboratoire sur puce micro-fluidique muni d'un générateur de flux appliquant plusieurs liquides différents.The microfluidic chip (1) comprises: • at least one input channel (4) connected to at least one well (2), each well (2) being associated with one output channel (5), • at least an analysis chamber (3a, 3b) connected to at least one well (2). • an analysis surface (6) comprising capture elements (6a) capturing biomarkers present in the liquid from the well (2) and representative of the cellular response of a biological sample contained in the well (2). A flow of liquid flows from the well (2) to the analysis surface (6) comprising the collection elements (6a). The liquid flow is between 0.1 μL / min and 2 mL / min and flows in a laminar fashion in the analysis chamber (3a, 3b). The chip (1) is part of a micro-fluidic lab-on-chip equipped with a flow generator applying several different liquids.

FR1762895A 2017-12-21 2017-12-21 MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP Expired - Fee Related FR3075817B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1762895A FR3075817B1 (en) 2017-12-21 2017-12-21 MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP
US16/956,632 US20200406263A1 (en) 2017-12-21 2018-12-21 Microfluidic chip, microfluidic lab-on-chip, fabrication method of one such chip and analysis method
PCT/FR2018/053524 WO2019122788A1 (en) 2017-12-21 2018-12-21 Microfluidic chip, microfluidic lab-on-a-chip, method for producing such a chip and analysis method
EP18845401.1A EP3728556A1 (en) 2017-12-21 2018-12-21 Microfluidic chip, microfluidic lab-on-a-chip, method for producing such a chip and analysis method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1762895A FR3075817B1 (en) 2017-12-21 2017-12-21 MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP
FR1762895 2017-12-21

Publications (2)

Publication Number Publication Date
FR3075817A1 FR3075817A1 (en) 2019-06-28
FR3075817B1 true FR3075817B1 (en) 2020-12-25

Family

ID=61873477

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1762895A Expired - Fee Related FR3075817B1 (en) 2017-12-21 2017-12-21 MICRO-FLUIDIC CHIP AND LABORATORY ON MICRO-FLUIDIC CHIP

Country Status (1)

Country Link
FR (1) FR3075817B1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112547148A (en) * 2020-12-09 2021-03-26 武汉纺织大学 Micro-fluidic target capture chip based on micro dam array, preparation method and application
CN113617403A (en) * 2021-08-05 2021-11-09 上海交通大学 Novel micro-fluidic chip of unicellular western blot

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
US10078075B2 (en) * 2011-12-09 2018-09-18 Vanderbilt University Integrated organ-on-chip systems and applications of the same
WO2013086329A1 (en) * 2011-12-08 2013-06-13 Research Triangle Institute Human emulated response with microfluidic enhanced systems
WO2014039514A2 (en) * 2012-09-05 2014-03-13 President And Fellows Of Harvard College Removing bubbles in microfluidic systems
EP3574985A1 (en) * 2013-12-20 2019-12-04 President And Fellows Of Harvard College Organomimetic devices and methods of use and manufacturing thereof
CA2934662C (en) * 2013-12-20 2024-02-20 President And Fellows Of Harvard College Low shear microfluidic devices and methods of use and manufacturing thereof
JP6131338B2 (en) * 2013-12-27 2017-05-17 株式会社朝日Ｆｒ研究所 3D micro chemical chip
US20160102283A1 (en) * 2014-10-09 2016-04-14 The United States Of America As Represented By The Secretary Department Of Health And Human Services Microfluidic device and system for precisely controlling and analyzing shear forces in blast-induced traumatic brain injuries

Also Published As

Publication number Publication date
FR3075817A1 (en) 2019-06-28

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