FR3059549B1

FR3059549B1 - COSMETIC USE OF AN EXTRACT FROM ULEX EUROPAEUS.

Info

Publication number: FR3059549B1
Application number: FR1670731A
Authority: FR
Inventors: Jade Olivier
Original assignee: Laboratoires Clarins SAS
Current assignee: Laboratoires Clarins SAS
Priority date: 2016-12-02
Filing date: 2016-12-02
Publication date: 2019-06-21
Anticipated expiration: 2036-12-02
Also published as: FR3059549A1

Abstract

L'invention concerne l'utilisation cosmétique d'un extrait d'Ulex europaeus pour protéger la peau des effets délétères des UV. L'invention concerne également l'utilisation cosmétique d'un extrait d'Ulex europaeus dans une composition cosmétique protégeant la peau des effets délétères des UV.The invention relates to the cosmetic use of an extract of Ulex europaeus to protect the skin from the deleterious effects of UV. The invention also relates to the cosmetic use of an extract of Ulex europaeus in a cosmetic composition protecting the skin from the deleterious effects of UV.

Description

COSMETIC USE OF AN EXTRACT FROM ULEX EUROPAEUS. The invention relates to the cosmetic use of an extract of Ulex europaeus to protect the skin from the deleterious effects of UV.

The skin, also called tegument (from the Latin tegumentum, cover), is the heaviest and most extensive organ of the body. In the adult man, its surface 2 represents 2 m for a mass of about 4 kg. Its thickness varies from 0.05 mm at the level of the eyelids to 4 mm at the level of the palms of the hands and the soles of the feet. However, the skin is not a simple body envelope; it provides multiple functions: protection function against external aggressions (mechanical, chemical, thermal and infectious stresses), fluid conservation, thermoregulation, exchanges, metabolic function (vitamin D synthesis, of lipids) and sensory function (touch, pain). All these functions are provided by a complex structure, associating various tissue elements (epithelial, connective, vascular and nervous), organized into three distinct compartments which are, from the surface to the depth: the epidermis, the dermis and 1'hypoderme.

The cell types encountered in the skin are numerous and have multiple functions:

The stratum corneum consists of corneocytes, cells filled with keratin, with high mechanical resistance and which are the end stage of differentiation of the keratinocytes of the underlying epidermis. The living epidermis, which is renewed every 28 days, consists of four cell types: keratinocytes (90%) that will form the very resistant stratum corneum, melanocytes (3%), which play a role in pigmentation and the defense of the skin against ultraviolet rays (UV), Merkel cells, which act as mechanoreceptors, and Langerhans cells, which provide the first line of immune defense against antigens in the environment.

The dermis is responsible for the biomechanical properties of the skin, firmness and elasticity. The dermis and epidermis are connected by a zone of adhesion, the dermo-epidermal junction (JDE), a very complex interface that controls the transfer of signals and nutrients between the dermis and the epidermis. The dermis contains the cutaneous vascular system and is irrigated by blood vessels. In the dermis, there are fibroblasts that synthesize an extracelullary matrix containing collagens (mainly type I and III collagens), proteoglycans and elastin, dendrocytes and cells of the immune system that can be recruited if necessary after stress or damage due to UV. The hypodermis acts mainly as a reservoir of hormones and growth factors. Thermal and mechanical insulation, it is composed of mesenchymal cells and adipocytes.

The intrinsic aging of the skin, or chronological aging, is the result of changes that take place over time. This intrinsic aging affects the skin and other organs in the same way. These changes are mainly the result of an accumulation of Reactive Oxygen Species (ROS) or telomere shortening, particular structures found at the ends of the chromosomes that make up our genetic heritage. In a similar way to the aging of other systems, chronological skin aging is characterized by the decline of a large number of skin functions: loss of proliferative capacities of keratinocytes, reduction of skin repair, decline of sensory functions, reduction of the number of epidermal cells of Langerhans, fall of the immune function and decrease of the functions of mechanical protection of the cutaneous barrier.

Extrinsic aging adds to intrinsic aging. 111 involves external events, such as air pollution, tobacco, but especially mainly UV radiation.

UV rays increase the expression of metalloproteinases (MMPs), which are responsible for the degradation of many matrix components. Among them are MMP-1, cleaving type I collagen, MMP-2 capable of degrading elastin or fibrillin as well as type VII collagen present in the JDE. MMP-3, on the other hand, has a broader substrate specificity and can degrade Type IV collagen (JDE), laminin (JDE), proteoglycans (dermis), fibrillin (dermis) and fibronectin (dermis). ).

In addition to the degradation of matrix collagens, UV also inhibits the synthesis of type I and III collagens: after irradiation of human skin, procollagen type I synthesis is significantly reduced within eight hours, and then reaches its maximum level of reduction. approximately 70% within 24 hours, before returning to a constitutive level three days later.

There are at least two mechanisms that explain this decrease in collagen synthesis: on the one hand, UV-irradiation of the skin activates AP-1 and NF-kappaB, two particular proteins called transcription factors that bind to DNA ( Deoxyribonucleic acid) and inhibit the expression of collagen I. On the other hand, this irradiation inhibits the TGF-beta signaling pathway, known to increase mainly the expression of collagen I.

The part of the solar radiation that penetrates our skin is composed of: visible rays (40% of the total); infrared (IR) (50%); and ultraviolet for the remaining 10%, mainly UV-A and UV-B.

UV, although a minority in percentage, is the most biologically active fraction of the solar spectrum. UV-C radiation (100-280 nm) is stopped by the ozone layer while UV-B (280-320 nm) is absorbed by the epidermis, and UV-A (320-400 nm) cross the epidermis and are absorbed by the dermis. UV-B is more energetic than UV-A, but much less represented than the latter in the light spectrum.

UV can react with DNA, directly by attacking DNA, and / or indirectly. When there is DNA attack, the four bases of DNA are attacked, a true "alphabet" of DNA carrying the genomic message: the purine bases, Guanine (G) and Adenine (A), and pyrimidine bases, Cytosine (C) and Thymine (T). These 4 bases of DNA are organized in complementary double helix where a purine base of a helix is associated (via weak bonds called "hydrogen") with its complementary pyrimidine base. It is the UV-B light, the most energetic, which is the most damaging for the genome: the UV-B photons are directly absorbed by the DNA, in which they induce photochemical reactions. The main targets are the pyrimidine bases (T and C) which, if they are located adjacent in the double helix of the DNA, react two by two to create new covalent bonds. The chemical structure of the bases is thus strongly altered since they are found in the form of photoproducts, classified in two categories: "cyclobutane pyrimidine", or CPD (with formation of pyrimidine dimers, ie dimers of C or T) and the photoproducts (called photoproducts 6-4), these designations referring to the nature of the bonds formed to create the damage. On the other hand, DNA absorbs very little UV-A and it has long been thought that these UV-A rays mainly intervene only in the production of reactive oxygen species, its effects on DNA. being then essentially only indirect. In fact, UV-A also generate photoproducts on DNA, the nature of these photoproducts induced by UV-A and UV-B radiation being very different. Indeed, exposure to UV-A only leads to the formation of cyclobutane pyrimidine dimers with a total absence of 6-4 photoproducts.

These DNA modifications are at the origin of cell death, or conversely of changes in DNA structures and mutations that can trigger the processes involved in the appearance of tumors.

The reactive oxygen species are oxygen radical derivatives and not radical radicals. In the presence of radiation, metals or enzymes, the oxygen (02) is able to capture an electron to give the radical superoxide · 02, which is an unstable radical. This radical is the substrate of essential enzymes, SOD (Superoxide Dismutases), which transform it into hydrogen peroxide (H2O2). Hydrogen peroxide can have several uses: - in the presence of metals, in particular Fe + ferrous iron, it is converted into a hydroxyl • OH radical by the Fenton reaction. The latter is very reactive and will oxidize very quickly the neighboring molecules, sometimes forming other free radicals; - Oxygenated water can also undergo detoxification reactions catalyzed by catalase, glutathione peroxidase or peroxyredoxins. Similarly, several compounds, including vitamins E and C, can interact with radicals and prevent their accumulation.

In addition, nitric oxide (NO ·) is a free radical with known physiological properties which, when it interacts with the superoxide · 02 anion, gives peroxynitrite, an extremely reactive and toxic compound. It is the imbalance between the formation of the ROS and the detoxification of the oxidized molecules which constitutes the "oxidative stress".

All cellular macromolecules are potentially the target of ROS: the nucleic bases are likely to be oxidized, in particular Guanine (G) leading to the formation of 8-oxo-guanine (8-oxo-G), at the origin of gene mutations. This phenomenon probably explains the genotoxicity of free radicals; proteins are also targets of ROS, especially certain amino acids (structural bases of proteins) such as cysteine, methionine and tyrosine. This oxidation plays a role in maturation and in signaling but can also lead to cellular toxicity, each oxidized molecule, unstable, becoming in turn a ROS, and can stabilize by oxidizing a neighboring molecule. A more "pure" oxidation of the proteins can further lead to their carbonylation and denaturation and thus to a loss of their function (or even to a "toxic" function of the protein); - ROS also react with unsaturated fatty acids, leading to the formation of hydroperoxides. In particular, lipid peroxidation affects the membranes of all skin cells and forms a compound called malondialdehyde (MDA). MDA is therefore a good marker of oxidative stress on lipids.

In conclusion, during extrinsic aging,

UV can generate harmful modifications of DNA; either directly, via the formation of cyclobutane dimers or photoproducts, or indirectly via ROS that can attack DNA (8-oxo-G formation) or other living molecules such as lipids (MDA formation), or proteins. The UV, by activating the MMPs, can further degrade the proteins of the extracellular dermal matrix, including fibrillin.

The Applicant has surprisingly demonstrated that an extract of Ulex europaeus protects the skin from the deleterious effects of UV.

By deleterious effects of UV on the skin is meant the effects of UV on the DNA of cutaneous cells, on epidermal cell membranes as well as on matrix proteins. The extract of Ulex europaeus protects the skin from the deleterious effects of UV, that is to say, it protects the DNA of cutaneous cells by limiting the formation of pyrimidine dimers, it protects epidermal cell membranes from oxidative stress and finally it protects the matrix proteins. As a result, the extract of Ulex europaeus prevents the loss of skin elasticity due to the sun. Loss of skin elasticity is understood to mean the involvement of the dermis fibers due to UV damage resulting in a wrinkled, possibly pigmented, appearance of the upper layers of the epidermis.

The present invention thus relates to the cosmetic use of an extract of Ulex europaeus to protect the skin from the deleterious effects of UV, to protect epidermal cell membranes from oxidative stress and to protect matrix proteins. The invention also relates to the cosmetic use of an extract of Ulex europaeus to prevent the loss of cutaneous elasticity due to the sun. The extract of Ulex europaeus may also be advantageously used in a cosmetic composition protecting the skin from the deleterious effects of UV. The use according to the invention is particularly suitable for application to healthy skin. In the context of the present invention, the term "healthy skin" denotes skin that does not exhibit skin pathology.

Ulex europaeus is a perennial evergreen shrub 1 to 2 meters tall belonging to the Fabaceae family. It is ash green and has many thick, upright stems. This bush is thorny with thorns robust, very vulnerable. The evergreen leaves are acicular and usually shorter than the spines. The many flowers of this plant are isolated or arranged in clusters and are between 1.5 and 2.5 cm long. These flowers have a characteristic smell of coconut oil. The fruit is a black oval pod, 1 to 2 cm wide. Very hairy, it is longer than the evergreen calyx and contains 1 to 7 seeds.

Ulex europaeus is a shrub legume native to Europe (rather central and western and especially Brittany, Britain and Galicia). Its range in Europe has expanded to include countries outside its area of origin, such as Austria, Belgium, the Czech Republic, Denmark, Sweden or Norway. Over the last century, its area has expanded considerably in Australia, New Zealand, Hawaii, Chile and Costa Rica, as well as along the Pacific and Atlantic coast in North America where it is considered an invasive species. From now on, Ulex europaeus would have been introduced on all the continents. In cold or temperate latitudes, it is present at sea level, in the tropics between 1000 and 2500 meters and at the equator between 2000 and 3500 meters.

The first use of Ulex europaeus stems from its nutritional qualities for livestock. It was called "alfalfa of the poor countries". Ulex europaeus flowers are also used which have a characteristic smell of vanilla and coconut flavors in food. They can be eaten raw or in salad, or marinated like capers. They are also used for wine design and to add color and taste to Irish whiskey.

This plant is not well known in herbal medicine, and there is little data available on its medical virtues. It is only in Spain that the traditional use of its flowers before maturity is described for their cardiotonic and depurative virtues of the liver. Compounds with antiviral activity were isolated from stems and leaves of Ulex europaeus. In addition, seed pectins are used in agglutination or blood grouping techniques.

Wood ash from Ulex europaeus was used to make soap. The bark of Ulex europaeus was used to make a dark green dye and the flowers gave yellow and green dyes. The cosmetic use according to the present invention preferably implements an extract of Ulex europaeus or a cosmetic composition comprising such an extract. The extract according to the invention is an extract of Ulex europaeus, preferably an extract of the aerial parts and more particularly the flowering aerial parts. Advantageously, this extract is an extract obtained by supercritical CO2 extraction using a caprylic / capric triglyceride cosolvent. The extract thus obtained is therefore contained in a caprylic / capric triglyceride co-solvent. It is obtained according to a process comprising at least the following stages: - Harvesting of the aerial parts (flowers) of Ulex europaeus which are dried. - Grinding in a refrigerated mill. - Extraction with supercritical CO2 using Co-solvent Caprique / Caprylic Triglyceride. The extraction is carried out at a pressure of 170-190 bar, and a temperature of 50-70 ° C, the amount of dried aerial parts being 35-45%. Evaporation under vacuum at a temperature of 60-80 ° C. - Adjustment to 0.3% of unsaponifiables with caprylic / capric triglyceride co-solvent. The extract of the aerial flower parts of Ulex europaeus used according to the invention is a cloudy yellow-green liquid with a characteristic green odor. It has the following analytical characteristics: - Relative density at 20 ° C: 0.94-.96 - Refractive index at 20 ° C: 1.446-1.452 - Acid number (mg KOH / g): <10 - Saponification index ( mg KOH / g): 320-350 - Peroxide value (meq Cq / kg): <10 - Unsaponifiable:> 0.2% - Water content <0.2% - Thin layer chromatography of unsaponifiable matter: presence of sterols.

Cosmetic use is understood to mean the use of a cosmetic composition, that is to say a formulation suitable for a cutaneous application, comprising the Ulex europaeus extract as well as cosmetically acceptable excipients. Thus, the compositions according to the invention may comprise one or more formulating agents or additives of known and conventional use in cosmetic compositions such as, by way of examples and in a nonlimiting manner, softeners, colorants film-forming active agents, surfactants, perfumes, preservatives, emulsifiers, oils, glycols, vitamins such as vitamin E, UV filters, etc. With his knowledge of cosmetics, the skilled person will know which formulating agents add to the compositions of the invention and in what quantities according to the desired properties.

The compositions according to the invention may be in any form known to those skilled in the field of cosmetology and dermatology with no other pharmaceutical restriction than the application on the face and on the body. Advantageously, the compositions according to the invention are in the form of a gel, a cream, a lotion, a mask, an oil, a milk, a spray, etc.

Preferably, the cosmetic composition according to the invention comprises from 0.01 to 10% of an extract of Ulex europaeus by weight of dry matter relative to the total weight of the composition. Advantageously, the composition comprises from 0.01 to 5% of an extract of Ulex europaeus by weight of dry matter relative to the total weight of the composition.

The Applicant has demonstrated that the Ulex europaeus extract used according to the invention as well as the cosmetic composition comprising it can be used by application to the skin to protect it from the deleterious effects of UV.

The present invention also relates to a method for protecting the skin from the deleterious effects of UV comprising the cutaneous administration of an extract of Ulex europaeus or a cosmetic composition comprising said extract.

The following examples relate, on the one hand, to the evaluation of the effect of an extract of Ulex europaeus on UVB stress by analysis of pyrimidine dimers, on the protection of epidermal cell membranes by assaying MDA and finally vis-à-vis the UVA stress by analysis of the amount of fibrillin 1. On the other hand the examples below relate to compositions object of the present invention.

The examples refer to the following figures in which: - Figure 1 shows the percentage of cells with pyrimidine dimers after exposure to UVB under different conditions. FIG. 2 represents the lipid peroxidation rate (MDA assay) in 10 mg of epidermis exposed to UVA under different conditions. FIG. 3 shows the immuno-detection of fibrillin in explants exposed to UVA under different conditions.

I. EVALUATION OF THE ACTIVITY OF A O'ULEX EUROPAEUS EXTRACT ON THE DELETE EFFECTS OF UV.

A. MATERIALS AND METHODS

Normal human skin samples (2 donors) were obtained from a tummy tuck of a 55-year-old woman and a breast reduction of a 62-year-old woman. A skin sample was stored at 0 ° C in formalin and frozen in OCT (Optimal Cutting Temperature Compound) at -32 ° C.

The skin fragments were deposited in inserts (1 cm 2) themselves placed on culture wells. Dulbecco's Modified Eagle Medium (DMEM) was added to the bottom of the wells, the passage of the culture medium to the inserts being carried out by slow diffusion between the two compartments by the intermediate of a porous membrane (12 μm).

B. REALIZATION OF OXIDATIVE STRESS MODEL

To be applied to the skin samples, two basic cosmetic formulations are prepared containing 0.2% and 0.3% Ulex europaeus extract in 100% 012-15 alkyl benzoate QSP. A placebo formula is performed with 100% 012-15 alkyl benzoate.

These 2 formulas are applied daily, topically, at a concentration of 2 mg / cm 2 from day 0 to day 4. 2 models of radiation are carried out on day 4 after the last application of the topicals: - model of irradiation by a session of UVB with 8 J / cm2 in order to obtain alterations of the DNA of the epithelial cells; - Irradiation model with a UVA session at 16 J / cm2 in order to obtain alterations of the cell membranes of the epithelium and dermal alterations, in particular with regard to the elastic fibers. On D5, each fragment was cut into 3: - formalin fixation and paraffin embedding for histological analysis - freezing at -32 ° C in OCT for immunohistochemical analysis - freezing at -32 ° C for MDA assay .

C.ANALYSIS 1) Histological analysis

Staining with hemalun-eosin makes it possible to check the possible cytotoxicity of the two formulas. 2) Immunohistochemical evidence of DNA damage by quantification of cyclobutane pyrimidine dimers (CPP) DNA of UV-disrupted epithelial cells was detected using an antibody anti-CPD (Abnova, clone KTM53, mouse monoclonal) (from 2 thymines, UV formation of cyclobutane pyrimidine dimers). The labeled cell nuclei were revealed in green fluorescence (fluorescent secondary antibody labeled FITC (Fluorescein IsoThioCyanate)). The cuts are mounted using the mounting medium

Vectashield (Vector, H-1300) to counterstain the DNA of the nuclei in red propidium ionide. Since the expression of pyrimidine dimers is not present on the whole of the skin, the number of labeled cells is counted at the level of 3 positive fields at magnification 40 and relative to the total number of cells of the epidermis des-dits fields. A calculation of the percentage of cells comprising DNA alterations is therefore performed. 3) Biochemical evidence of lipid peroxidation of cell membranes

The lipid peroxidation at the cell membranes generates MalonDiAldehyde (MDA) compounds and is a marker of oxidative stress. Peroxidation of lipids is assayed using a SIGMA kit, reference MAK085.

For this analysis, 10 mg of epidermis are extracted in 300 μl of buffer containing BHT (butylated hydroxytoluen). After centrifugation, the extraction supernatant is used to assay the binding of 2 molecules of thiobarbituric acid (TBA) on an MDA molecule. The pink chromogen thus produced is determined by sprectrophotometry at 532 nm.

A standard curve is made using a standard 4.17 M MDA solution. The amount of MDA is based on the weight of the epidermis. The results are therefore expressed in nM of MDA / mg of epidermis. 4) Immunohistochemical Detection of Fibrillin Immunodetection is performed using a 3-layer indirect immunoperoxidase technique (ABC Peroxidase kit, Vector Laboratories) and revealed in AEC. The evaluation is carried out using semi-quantitative scores (0 to 4) indicating the intensity of the labeling of fibrillin 1 in the elastic fibers.

D.RESULTS 1) Histological analysis At 0.3%, the extract of Ulex europaeus does not induce a cytotoxic effect on the skin. At 0.2% a moderate cytotoxic effect is observed. 2) Immunohistochemical detection of DNA damage by quantification of cyclobutane pyrimidine dimers (CPP)

After treatment with UVB, 21.6% of the keratinocytes contain the presence of pyrimidine dimers (testifying to the alteration of the DNA) relative to the control. Pre-treatment of skins with 0.3% Ulex europaeus extract prevents DNA damage by protecting keratinocytes with only 6.6% CPD in the epidermis. The protection obtained thus corresponds to a reduction in the formation of pyrimidine dimers of approximately 53% compared with placebo. Pre-treatment of

skin by the concentration of 0.2% Ulex europaeus extract does not protect the skin vis-à-vis alterations by UVB. 3) Biochemical evidence of lipid peroxidation of cell membranes

The

The level of MDA measured after application of the products alone is not different from that measured in the control skin.

After treatment with UVA, an increase in peroxidation of membrane lipids is observed with 700.5 nM / mg MDA compared with

control skin where the level is 343.5 nM / mg (2-fold increase).

Pre-treatment of the skin with 0.2% Ulex europaeus extract reduces lipid peroxidation with only 203.5 nM / mg MDA in the epidermis. This corresponds to a decrease of about 70% compared to placebo. With 0.3% Ulex europaeus extract the result is no different from the placebo. 4) Immunohistochemical Detection of Fibrillin

After irradiation with UVA, a decrease in the amount of fibrillin-1 is observed in the superficial dermis with a score of 2.9 versus 3.8 for the control skin. The extract of Ulex europaeus at 0.2% and 0.3% can protect the elastic tissue with semi-quantitative scores of 3.3 to 3.4 respectively. This corresponds respectively to a fibrillin protection rate of 132% and 136% compared to placebo.

III.EXAMPLES OF COSMETIC COMPOSITIONS

PROTEIN FACE CREAM O, "o ACRYLATES / C10-30 ALKYL ACRYLATE CROSSPOLYMER 0.35 PHENOXYETHANOL ............................... 0,40 GLYCERIN ..................................... 5,0 0 XANTHAN GUM ... ............................... 0.2 0 BUTYLOCTYL SALICYLATE ............. ........... 5,00 BUTYL METHOXYDIBENZOYLMETHANE ............... 3,00 CETEARYL ALCOHOL & CETEARYL GLUCOSIDE ....... 3,00 HOMOSALATE .................................. 9.0 0 DIMETHICONE .......... ....................... 3.0 0 02-15 ALKYL BENZOATE .................. ..... 3,00 TOCOPHEROL .................................. 0,0 6 TOCOPHERYL ACETATE. ......................... 0,20 BUTYROSPERMUM PARKII (SHEA) BUTTER .......... 5,00 DIMETHICONE / VINYL DIMETHICONE CROSSPOLYMER & SILICA & BUTYLENE GLYCOL .................... 1,00 TROMETHAMINE ................. ............... 0,25 PERFUME ............................... ....... 0,50 ULEX EUROPAEUS EXTRACT ...................... 0,3 DEMINERALIZED WATER .......... ................ QSP 100

MILK PROTECTIVE BODY O, "o ACRYLATES / C10-30 ALKYL ACRYLATE CROSSPOLYMER 0.20 CARBOMER ............................... ..... 0.10 02-15 ALKYL BENZOATE ........................ 3.00 BUTYLENE GLYCOL ........ ...................... 5,00 DISODIUM EDTA ....................... ......... 0,20 POTASSIUM SORBATE ............................ 0,10 PHENOXYETHANOL .... .................................. 0.60 TROMETHAMINE ................... .............. 0,40 POTASSIUM CETYL PHOSPHATE .................... 1,00 TOCOPHERYL ACETATE ..... ...................... 0,20 VP / EICOSENE COPOLYMER ..................... ... 2.00 ETHYLHEXYL METHOXYCINNAMATE & BHT ............ 7.50 OCTOCRYLENE ....................... ........... 10,00 BUTYL METHOXYDIBENZOYLMETHANE ................ 3,00 ETHYLHEXYL SALICYLATE ............. ........... 3,00 DIMETHICONE .................................. 8 , 00 ALOE BARBADENSIS LEAF JUICE & SODIUM BENZOATE 1.00 DIMETHICONE .................................. 8, 00 ULEX EUROPAEUS EXTRACT ....................... 0,3 0 PERFUME ...................................... 0,40 DEMINERALIZED WATER ..... ...................... QSP 100

MINERAL PROTECTIVE BASE O, "o DISODIUM EDTA ............................... 0.10 SODIUM CHLORIDE ... .......................... 1,00 PHENOXYETHANOL .................... .......... 0.60

AQUA / WATER / WATER & STYRENE / ACRYLATES COPOLYMER & PENTYLENE GLYCOL & AMMONIUM HYDROXIDE ..... 12.00 DIMETHICONE & TITANIUM DIOXIDE & Polyglyceryl-3

POLYDIMETHYLSILOXYETHYL DIMETHICONE & ALUMINUM HYDROXIDE & STEARIC ACID .................... 20.00 NYLON-12 ...................... .............. 3,00 DIMETHICONE & PEG / PPG-18/18 DIMETHICONE ..... 3,00 DIMETHICONE ................................. 5,00 ISODODECANE ................................. 5,0 0 ALCOHOL ........ ............................. 4,0 0 ULEX EUROPAEUS EXTRACT .............. ........ 0.30 PERFUME ...................................... 0.4 0 DEMINERALIZED WATER ........................... QSP 100

Claims

1) Cosmetic use of an extract of ülex europaeus to protect the skin from the deleterious effects of UV.

2} The use of claim 1 for protecting epidermal cell membranes from oxidative stress.

3) Use according to claim 1 for protecting the matrix proteins.

4. Cosmetic use according to any one of the preceding claims, for preventing the loss of skin elasticity due to the sun.

5} Cosmetic use of an extract of Ulex europaeus in a cosmetic composition protecting the skin from the deleterious effects of UV, said deleterious effects of UV on the skin are selected from the protection of the epidermal cellular membranes of oxidative stress, the protection of proteins matrix and the prevention of loss of skin elasticity due to the sun.

6) Cosmetic use according to any one of the preceding claims, wherein the Ulex europaeus extract is an extract of the aerial parts of the plant.

7) Cosmetic use according to any one of the preceding claims, wherein the Ulex europaeus extract is an extract of the flowering aerial parts of the plant.

8) Cosmetic use according to any one of the preceding claims wherein the Ulex europaeus extract is an extract obtained by supercritical COz extraction.

9) Cosmetic use according to any one of the preceding claims wherein the Ulex europaeus extract is an extract contained in a caprylic / capric triglyceride co-solvent.

10) Cosmetic use according to claims 5 and following in which the cosmetic composition comprises from 0.01 to 10% of said Ulex europaeus extract by weight of dry matter relative to the total weight of the composition, preferably from 0.01 to 5% of said extract by weight of dry matter relative to the total weight of the composition.

11) Cosmetic use according to claims 5 and following wherein said cosmetic composition further comprises one or more formulating agents or additives such as softeners, dyes, film-forming agents, surfactants, perfumes, preservatives, emulsifiers, oils, glycols, UV filters, and vitamins.