FR3057276B1 - Procede et dispositif de capture et/ou concentration de microorganisme. - Google Patents

Procede et dispositif de capture et/ou concentration de microorganisme. Download PDF

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Publication number
FR3057276B1
FR3057276B1 FR1659746A FR1659746A FR3057276B1 FR 3057276 B1 FR3057276 B1 FR 3057276B1 FR 1659746 A FR1659746 A FR 1659746A FR 1659746 A FR1659746 A FR 1659746A FR 3057276 B1 FR3057276 B1 FR 3057276B1
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France
Prior art keywords
liquid
lysis
washing
allowing
path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1659746A
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English (en)
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FR3057276A1 (fr
Inventor
Frederic Foucault
Edgar Minassian
David Mosticone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Priority to FR1659746A priority Critical patent/FR3057276B1/fr
Priority to PCT/FR2017/052755 priority patent/WO2018069612A1/fr
Priority to EP17794362.8A priority patent/EP3523624B1/fr
Priority to US16/336,441 priority patent/US12024702B2/en
Publication of FR3057276A1 publication Critical patent/FR3057276A1/fr
Application granted granted Critical
Publication of FR3057276B1 publication Critical patent/FR3057276B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif de capture et/ou concentration de microorganisme ou de protéine issue dudit microorganisme, comprenant - un porte-cartouche (300) comprenant : - au moins un moyen d'insertion (304) permettant à une cartouche (100) d'être insérée dans le porte-cartouche, - au moins un premier conduit d'admission (301) permettant l'entrée d'un échantillon liquide, - au moins un deuxième conduit d'admission (303) permettant l'entrée d'un liquide de lavage et/ou d'un liquide de lyse et/ou un liquide d'élution, au moins un conduit d'évacuation (302) permettant la sortie de l'échantillon liquide et/ou du liquide de lavage ou de lyse. - une cartouche (100) comprenant : - au moins un moyen d'insertion (106) permettant à la cartouche d'être insérée dans au moins deux positions différentes, une première position selon un chemin fluidique dit de « capture/concentration » et une deuxième position selon un chemin fluidique dit de « lyse et/ou lavage et/ou élution». - un module réactionnel comprenant : - au moins une voie d'admission (201) de l'échantillon liquide et/ou du liquide de lavage et/ou de lyse et/ou d'élution, - au moins une voie d'évacuation (202) de l'échantillon liquide et/ou du liquide de lavage et/ou de lyse et/ou d'élution, - un support de capture et/ou concentration (104) disposé entre la voie d'admission et la voie d'évacuation de telle sorte que tout ou partie de l'échantillon ou des liquides le traverse.
FR1659746A 2016-10-10 2016-10-10 Procede et dispositif de capture et/ou concentration de microorganisme. Active FR3057276B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1659746A FR3057276B1 (fr) 2016-10-10 2016-10-10 Procede et dispositif de capture et/ou concentration de microorganisme.
PCT/FR2017/052755 WO2018069612A1 (fr) 2016-10-10 2017-10-09 Procédé et dispositif de préparation d'échantillons
EP17794362.8A EP3523624B1 (fr) 2016-10-10 2017-10-09 Procédé et dispositif de préparation d'échantillons
US16/336,441 US12024702B2 (en) 2016-10-10 2017-10-09 Method and device for preparing samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1659746A FR3057276B1 (fr) 2016-10-10 2016-10-10 Procede et dispositif de capture et/ou concentration de microorganisme.
FR1659746 2016-10-10

Publications (2)

Publication Number Publication Date
FR3057276A1 FR3057276A1 (fr) 2018-04-13
FR3057276B1 true FR3057276B1 (fr) 2020-10-23

Family

ID=58162710

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1659746A Active FR3057276B1 (fr) 2016-10-10 2016-10-10 Procede et dispositif de capture et/ou concentration de microorganisme.

Country Status (1)

Country Link
FR (1) FR3057276B1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976824A (en) * 1993-11-24 1999-11-02 Abbott Laboratories Method and apparatus for collecting a cell sample from a liquid specimen
US9028777B2 (en) * 2010-03-23 2015-05-12 Fluidigm Corporation Automated cellular material preparation
US20130071872A1 (en) * 2011-09-15 2013-03-21 Lewis Ho Device and method for single-used matrix sampling
FR2997703B1 (fr) * 2012-11-07 2016-12-30 Biomerieux Sa Procede de traitement d'au moins un echantillon biologique

Also Published As

Publication number Publication date
FR3057276A1 (fr) 2018-04-13

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