FR3034103B1 - Procede de mesure de la concentration de microorganismes dans un liquide - Google Patents

Procede de mesure de la concentration de microorganismes dans un liquide Download PDF

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Publication number
FR3034103B1
FR3034103B1 FR1552556A FR1552556A FR3034103B1 FR 3034103 B1 FR3034103 B1 FR 3034103B1 FR 1552556 A FR1552556 A FR 1552556A FR 1552556 A FR1552556 A FR 1552556A FR 3034103 B1 FR3034103 B1 FR 3034103B1
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FR
France
Prior art keywords
sample
microorganisms
liquid
concentration
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1552556A
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English (en)
Other versions
FR3034103A1 (fr
Inventor
Pierre Sanchez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Couturier Xavier
Original Assignee
Couturier Xavier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Couturier Xavier filed Critical Couturier Xavier
Priority to FR1552556A priority Critical patent/FR3034103B1/fr
Publication of FR3034103A1 publication Critical patent/FR3034103A1/fr
Application granted granted Critical
Publication of FR3034103B1 publication Critical patent/FR3034103B1/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0092—Polarisation microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/075—Investigating concentration of particle suspensions by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N2015/0687—Investigating concentration of particle suspensions in solutions, e.g. non volatile residue
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Optics & Photonics (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Signal Processing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un dispositif de mesure de la concentration de microorganismes dans un échantillon (12) de liquide, comportant une source de lumière (11), un support d'échantillon (17), un capteur d'images (14), un dispositif optique (13) situé entre ledit support d'échantillon (17) et ledit capteur d'images (14), un microprocesseur et un programme de comptage, ledit dispositif optique (13) étant constitué d'une seule lentille transparente. La présente invention concerne également un procédé de contrôle du développement de microorganismes dans un liquide, caractérisé en ce qu'il comporte les étapes suivantes : - prélèvement d'un échantillon (12) dudit liquide, - introduction dudit échantillon (12) dans un dispositif selon l'une des revendications précédentes, - détection d'une image dudit échantillon (12) par ledit capteur d'images (14), - détermination de la concentration de microorganismes contenus dans ledit échantillon par comptage de microorganismes sur ladite image, par le moyen dudit programme.
FR1552556A 2015-03-26 2015-03-26 Procede de mesure de la concentration de microorganismes dans un liquide Expired - Fee Related FR3034103B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR1552556A FR3034103B1 (fr) 2015-03-26 2015-03-26 Procede de mesure de la concentration de microorganismes dans un liquide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1552556A FR3034103B1 (fr) 2015-03-26 2015-03-26 Procede de mesure de la concentration de microorganismes dans un liquide

Publications (2)

Publication Number Publication Date
FR3034103A1 FR3034103A1 (fr) 2016-09-30
FR3034103B1 true FR3034103B1 (fr) 2022-04-22

Family

ID=53541743

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1552556A Expired - Fee Related FR3034103B1 (fr) 2015-03-26 2015-03-26 Procede de mesure de la concentration de microorganismes dans un liquide

Country Status (1)

Country Link
FR (1) FR3034103B1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3067110A1 (fr) * 2017-06-01 2018-12-07 Centre National De La Recherche Scientifique (Cnrs) Dispositif et procede de detection et d'imagerie d’elements biocontaminants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1269244A2 (fr) * 2000-04-03 2003-01-02 Pocketscope.com LLC Lentilles et utilisations, notamment avec des microscopes
US9304280B2 (en) * 2013-03-14 2016-04-05 The Regents Of The University Of Michigan Compact lens system and array

Also Published As

Publication number Publication date
FR3034103A1 (fr) 2016-09-30

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