FR3001633A1 - Cosmetic use of a combination comprising at least a first and a second nutritional agents for inducing weight loss to restore, maintain and promote good esthetic condition of skin and to promote weight loss - Google Patents

Abstract

Cosmetic use, during a weight loss nutritional diet, of a combination comprising at least a first and a second nutritional agents for inducing weight loss to restore, maintain and promote good esthetic condition of skin and/or its annexes and to promote weight loss, is claimed. The nutritional agents are implemented simultaneously, separately or sequentially and are, respectively, administered at a dose for a period of time unable to induce weight loss. Independent claims are included for: (1) a cosmetic method for restoring, maintaining and promoting good esthetic condition of the skin and hair and/or the annexes, and promoting weight loss, during a weight loss nutritional diet, comprising administering, to an individual, the combination of the first and second nutritional agents; and (2) an assembly to implement the method, comprising, separately packaged, a first combination comprising at least glucomannan, a second combination comprising vitamins B3 and B8, Lactobacillus rhamnosus and fibers, and a third combination comprising calcium, glucosamine, phenolic extract of pine and green tea extract. ACTIVITY : Anorectic; Anabolic. MECHANISM OF ACTION : None given.

Description

The present invention relates to the field of food supplements intended to accompany slimming diets. In particular, the present invention relates to a use of a combination of at least two, at least first and second, nutritional active agents distinct from each other, in order to concomitantly exert an effect cosmetic on the skin, or its annexes, and promote weight loss. Also, the present invention relates to uses and methods for, concomitantly, (i) restoring, maintaining or promoting a good aesthetic condition of the skin and / or its appendages and (ii) promoting weight loss, as well as assemblies suitable for these uses and methods.

The recommended weight range for an individual can be estimated by a calculation of the BMI (Body Mass Index or BMI) index. The BMI is one of the norms of weight normality which is calculated by dividing the weight of the individual (in Kg) by the square of his height (in m). A BMI considered normal for a healthy human being is between 18.5 and 24.9 in an adult woman and between 23 and 25 in an adult male. This is obviously a calculated indication and practitioners sometimes confirm this first indication by additional medical and biological examinations to confirm the actual physiological normality of weight that may vary depending on the morphology of the individual and these others. physiological parameters. Weight-loss or slimming diets designed to promote weight loss to achieve a BMI qualified as normal or ideal are multiple and address this issue in different ways. Thus, we can see that there are so-called vegetarian, low calorie, hyper-protein diet, etc. In general, a slimming or slimming diet comprises three phases, or periods of time, successive: 1- A first phase, called acute phase of intervention or attack, characterized by weight loss is a short phase with generally a strong negative energy balance. 2- A second phase, called the stabilization or cruise phase, is characterized by weight management involving an active phase of intervention of equilibrium, but less negative in terms of energy balance. It corresponds to a phase where weight loss is actively engaged and under control and that the target in terms of ideal BMI or acceptable target target weight should be almost reached. 3- A third phase, called the maintenance phase, is defined by the objective of maintaining an ideal body weight while introducing a dietary diversification, or even allowing certain differences on the food balance. It consists in particular to avoid any rebound or to regain weight or a fat mass and to ensure a balanced diet including the supply of essential nutrients. Strict monitoring of diets designed to regain an equilibrium weight that is acceptable to the person who decides to stick to it can sometimes lead to nutritional imbalances and, in the long term, even severely deprive the body of important nutrients for its good functioning. Among these nutrients, there are elements that the body is able to synthesize (they are called non-essential) and elements that it is not able to synthesize and that can only be brought by food ( speaks of essential nutrients). There is an intermediate class of nutrients that the body can synthesize under certain conditions (for example vitamin D3 under the effects of a reasoned solar exposure) but that the food can also contribute to bring when the physiological conditions of its production by the organism are no longer insured: we speak of semi-essential nutritional elements. Some weight loss diets cause some of the essential or semi-essential nutrients to be in short supply. We are talking about deficiencies. A report from ANSES (Opinion of the National Agency for Food Safety, the Environment and Labor on the demand for risk assessment of dietary weight-loss practices, 4 May 2011. ANSES - Reference n ° 2009-SA-0099) indicates, for example, that the majority of the practices of slimming lead to notable deficiencies in terms of contributions in particular, fibers, vitamins C, D or E, magnesium, iron, etc. It is also known that, like any organ of the human body, skin, hair, nails, teeth, or gums are particularly sensitive to nutritional intake.

Thus, for example, research in cosmetics and dermatology has made it possible to identify that, beyond its action with respect to scurvy, vitamin C plays a vital role in increasing the synthesis of collagen in the skin. that vitamin E acts to protect the skin cells from oxidative stress and in particular that associated with the aggressions of the sun, that the vitamin D3 makes it possible to limit the inflammatory processes cutaneous, or that vitamin B8 makes it possible to promote the growth of the hair, etc. However, with the exception of clinical data obtained on patients undergoing massive weight changes, there is little or no published scientific evidence regarding the impact on the skin or hair of the diet in non-obese patients. . Common knowledge refers, mainly, to the unsightly effects of sudden weight loss, such as the appearance of unsightly skin folds in the neck and lower part of the face, as well as stretch marks reflecting a disturbance of the skin. Collagen fibers consecutively to rapid successive weight changes (succession of setting phases and weight loss, also called the yoyo effect).

Thus, it appears useful, during the follow-up of slimming or slimming diets, in particular of low-calorie diets, to take care to supply in a continuous and sufficient way nutrients favoring a good protection and a good development of the skin, the dander, the teeth or the mucous membranes, while achieving an expected balanced weight, safely and effectively.

It is also necessary to find an acceptable balance between the nutritional restriction and the needs of the skin and appendages but also other organs and those of other cells, including fat, to achieve a goal of a return to normal physiological weight considered normal for a healthy individual while maintaining an aesthetic appearance of the skin or its annexes in good condition.

There is also a need for new treatments or dietary supplements intended to remedy, and in particular, reduce, prevent or treat aesthetic defects of the skin and / or its appendages that may be consecutive to the follow-up of dieting or slimming. The object of the present invention is to satisfy these needs.

Thus, according to a first subject, the present invention relates to a cosmetic use, during a diet slimming diet, of a combination of at least a first and a second nutritional assets capable of inducing weight loss to (i) restore maintain or promote a good aesthetic state of the skin and / or its appendages, and (ii) promote weight loss, said active agents being carried out simultaneously, separately or sequentially and being respectively administered at a given dose. and / or for a period of time incapable of inducing weight loss. A combination of assets of the invention comprises at least two assets, the assets being distinct from one another. Unexpectedly, the inventors have determined that it is possible to establish a range of assets or combinations of assets to be used as dietary supplements during a diet and to promote a return to an equilibrium weight without to compromise the normal development of the skin, skin appendages, teeth or mucous membranes, or even significantly improving it.

The present invention is based on the advantageous observation that it is possible to use assets or combinations of active agents, which are distinct from one another, which are known to be active to induce weight loss, but at doses and / or or times of supplementation lower than those clinically evaluated as being effective when used alone for the sole purpose of weight loss, for, concomitantly, (i) restoring, maintaining or promoting a good aesthetic condition of the skin and / or its annexes, and (ii) promote weight loss. According to another of its objects, the present invention relates to a cosmetic method for (i) restoring, maintaining or promoting a good aesthetic state of the skin and hair and / or their appendages and (ii) promoting weight loss, when of a dieting nutritional diet, said method comprising at least one step of administering, to an individual in need thereof, at least one combination of at least a first and a second nutritional asset capable of inducing a weight loss, said active ingredients being carried out simultaneously, separately or sequentially and being respectively administered at a dose and / or for a period of time incapable of inducing weight loss. According to yet another of its objects, the present invention relates to an assembly, in particular for implementing a method or a use of the invention, comprising, conditioned separately, a first combination of active agents comprising at least glucomannan; a second combination of active ingredients comprising at least vitamins B8 and B3, Lactobacillus rhamnosus, and fibers; and a third combination of actives comprising at least calcium, glucosamine, phenol pine extract, and green tea extract. According to an alternative embodiment, the uses and methods of the invention can implement at least two, at least a first and a second combination of active ingredients as defined above, and preferably at least three, at least first, second, and third combinations of assets. These different combinations of assets can be implemented simultaneously, separately or sequentially. For the purposes of the invention, the expression "skin and its appendages" is intended to denote all of the skin of the body, including the scalp, as well as the nails, the hair, and the mucous membranes, such as the lips. Preferably, the present invention is directed to the skin of the body, the nails or the hair. The present invention is intended for humans, preferably adults, with or without overweight, and who follow a diet, preferably a diet low calorie diet. Thus, the uses and methods of the invention are used in individuals who follow a diet slimming or slimming. For the purpose of the invention, the term "simultaneous, separate or sequential use" with respect to a combination of assets of the invention, taken as a single entity, an oral administration of the constituent the combination, either together in the same composition, or together in separate compositions, or successively in time, in separate compositions. When administered together in separate compositions of the constituent active ingredients a combination of the invention, the compositions are administered one after the other so that the administration is considered to be carried out in the same unit of time and constitutes a single action. When sequentially being delivered in separate compositions, the time interval between each of the administrations is such that administrations are considered to be performed in different time units. Such a time interval can range from a few tens of minutes to a few hours. Preferably, such a time interval can range from 1, or even 2, or even 3 or 4, 5, 6, 7 or 8 hours, so that the compositions are considered to be administered at different times of a same day. In a combination of assets of the invention, the assets constituting the combination can therefore be administered at different times of the same day. The invention also relates to the use of a plurality of combinations of assets, in particular at least two combinations, and preferably at least three combinations of assets as defined above or below. These combinations can also be administered simultaneously, separately or sequentially as indicated above. For the purposes of the invention, the term "nutritional active ingredient capable of inducing weight loss" means any nutritional active ingredient whose effect on the weight loss of a human individual has been clinically validated. Those skilled in the art may, for example, refer to Pittler & Ernst (Pittler MEI, Ernst E., Dietary supplements for body-weight reduction: a systematic review, Am J Clin Nutr. 2004 Apr; 79 (4) : 529-36) for the valuation of such assets. For the purposes of the invention, the term "diet slimming or slimming" means any diet whose primary purpose is to ensure weight loss in an individual who follows. Examples of such diets include hyperproteic type diets (eg MAYO, ATKINS, California, FRICKER, DURAN, etc.); hyperglucidic-type diets (eg LEMON DETOX, cabbage soup, WEIGHT WATCHER, etc.); hyperlidic diets (eg MONTIGNAC diets, chrononutrition, MIAMI, etc.); see opinion PANSES May 4, 2011. For the purposes of the invention, by "promote weight loss" with respect to a combination of assets, the property to directly induce such weight loss or to enhance the weight loss effect achieved by a dieting nutritional diet. For the purposes of the invention, the term "cosmetic" is intended to include assets, combinations of such assets, their uses, methods implementing them or sets comprising them, which are capable of improving, protecting, promoting, maintaining or restore the appearance and the good aesthetic state of the skin and its appendages.

For the purposes of the invention, the term "prevent", with respect to an aesthetic defect of the skin or its appendices, reduces the risk of occurrence of this defect. Indications The invention advantageously makes it possible, simultaneously, (i) to restore, maintain or promote a good aesthetic state of the skin and / or its appendages and (ii) to promote weight loss in an individual following a diet slimming diet. Thus, the present invention proves particularly advantageous for reducing, preventing and / or treating an aesthetic defect in the skin and / or its appendages that may be associated with or occur during the follow-up of a nutritional slimming diet.

In particular, the invention is advantageous for restoring, maintaining or promoting a synthesis of collagen or connective tissue, to protect the skin cells against oxidative stress to promote the quality and growth of hair, to promote the reduction of the fall of hair, to restore, maintain or promote normal development of the skin, mucous membranes, nails or hair, to restore, maintain or promote the beauty of the face or hair, to restore, maintain or promote normal pigmentation of the skin or hair, or to restore, maintain or promote normal function of skin, mucous membranes, nails or hair. In particular, the invention advantageously makes it possible to exert a nutritive effect on the skin, nails or hair, and / or to stimulate defense or differentiation and / or growth functions of the skin and / or hair and / or nails and / or mucous membranes and / or teeth. According to another embodiment, the invention can be implemented to promote weight loss and concomitantly improve the aesthetic appearance of the face, teeth, smile, mucous membranes, skin or its annexes.

As an example of aesthetic defects of the skin and / or its appendages that may be associated with the monitoring of a dieting nutritional regime, mention may be made of wrinkles and fine lines, wilted skin, loose skin, lack of elasticity and / or tone of the skin, thinning of the dermis and / or degradation of collagen fibers which causes the appearance of soft and wrinkled skin. According to another preferred embodiment, the invention is particularly suitable for reinforcing the mechanical properties of the skin, in particular for combating wilted, soft, distended, sagging and / or thinned skin, and / or strengthening and / or restoring the elasticity or the firmness of the skin.

According to another preferred embodiment, the invention is suitable for preventing, treating or reducing an aesthetic defect in the skin chosen from thinning of the skin, loss of firmness, loss of elasticity, loss of density, or loss of skin tone, the appearance of a marked microrelief of the skin, the formation and / or presence of fine lines and / or wrinkles, an alteration of the radiance of the complexion of the skin, a papery appearance of skin, sagging skin, or wilting of the skin. According to another preferred embodiment, the invention is also suitable for ensuring and maintaining the presence of a physiological hydration of the skin and its appendages during a dietary diet slimming. Thus, the invention is suitable for preventing, treating or reducing an aesthetic defect in the skin resulting from a manifestation of skin water deficiency, following a dieting nutritional regime, such as a rough-to-touch, wrinkled skin, even covered with scales. According to a preferred embodiment, the invention is also suitable for preventing, treating or reducing the skin withered, the lack of elasticity, flexibility and / or tone of the skin, the rough feel, the presence of crevices, desquamation , the presence of scales, or the wrinkles and fine lines associated with dry skin. According to another embodiment, the invention is particularly suitable for preventing, treating or reducing an aesthetic defect of the skin affecting the complexion and / or the pigmentation of the skin and following a diet slimming diet.

Pigmentation defects of the skin can manifest in a variety of different forms. However, skin pigmentation defects concerned by the invention may be defined as involving the occurrence of at least one cutaneous zone having a darker or lighter color than the average color of the cutaneous surface of the examined individual. This zone can be of macroscopic or microscopic size.

For the purposes of the invention, the term "skin pigmentation defects" means any occurrence of a change in the color, or the complexion, of all or part of the surface of the skin, which includes a change global or local complexion of the skin, as well as hyperpigmentary defects, hypopigmentary or dyspigmentaires.

The present invention also relates to aesthetic disorders of the skin affecting the skin tone and may occur during a diet slimming diet. Thus, an implementation of the invention may be suitable for preventing, reducing and / or treating a heterogeneity of the complexion of the skin, or even for improving the complexion of the skin. The invention advantageously allows to promote and maintain the radiance of the complexion of the skin, and in particular a uniform complexion. According to one embodiment, a use according to the invention can make it possible to treat and / or prevent an alteration of the radiance of the complexion, or a loss of the radiance of the complexion of the skin. Use according to the invention may make it possible to give the skin a uniform, luminous, more radiant, even radiant complexion, reflecting a healthy skin.

The invention advantageously makes it possible to prevent, reduce and / or treat a dull skin complexion, a complexion of dull skin, a complexion of heterogeneous skin, imperfections of the skin, in particular chosen from pimples, scabs, dyschromias, blackheads, or to prevent, reduce and / or treat a waxy, yellowish, greyish or ashen or even sickly complexion.

According to yet another embodiment, the invention is suitable for reducing, preventing and / or treating the aesthetic defects of keratinous fibers, in particular the hair or the eyelashes, and which may occur during a diet slimming diet. Thus, the invention is suitable for cosmetically improving, and even preventing and / or treating soft, brittle, dull hair, refining forks, and improving the vigor of keratinous fibers, the volume of the hair and its shine. In particular, the present invention aims to improve the aesthetic appearance of the hair, in particular by limiting or delaying the appearance of soft, brittle or dull hair. According to one embodiment, the invention also aims to keep the eyelashes in good condition and / or improve their condition and / or their appearance. According to another embodiment, the present invention aims to induce and / or stimulate the growth of human keratinous fibers, in particular the hair and the eyelashes, and / or to slow down and / or delay their fall, and / or to increase their density.

According to one embodiment, the invention also aims to induce and / or stimulate the growth of the eyelashes and / or increase their density. According to one embodiment, the present invention aims to prevent and / or treat the reduction in the density of keratinous fibers, and in particular the hair, and the decrease in the diameter of the hair rods. From a cosmetic standpoint, the present invention advantageously makes it possible to increase the capillary density in order to obtain abundant hair. Assets and combinations of assets A combination of the invention comprises at least two assets, at least one first and one second, necessarily distinct from each other. The active agents that are suitable for the invention are nutritional assets capable of inducing weight loss and may be chosen from vitamins, water-soluble and insoluble vegetable fibers, probiotic microorganisms, minerals, vitamins, prebiotic compounds, glucosamine and its derivatives, plant extracts or amino acids. The nutritional assets capable of inducing weight loss and suitable for the invention may also be chosen from conjugated linoleic acids (or CLAs), licorice extracts, chili extracts, saffron extracts, milk proteins, vegetable proteins, L-carnitine, chitosan, guar gum, chromium, garcinia cambogia, Psyllium, yerba mate, guarana, common bean or Phaseolus vulgaris L., Nopal fiber or Opuntia Ficus Indica. The active agents are each used in combinations of the invention at a dose and / or used for a duration such that, if they are used alone at this dose or during this period, they do not have slimming activity validated clinically. These doses and durations are described as ineffective. In other words, at these doses or durations the assets of the invention are incapable of inducing weight loss. On the other hand, the combinations of the invention comprising these active agents in ineffective amounts and / or used during ineffective periods are advantageously endowed with clinical efficacy both for promoting weight loss and also for reducing, preventing or treating defects. aesthetics of the skin and / or its appendages related to the follow-up of a slimming diet.

Vitamins The vitamins that are suitable for the invention may especially be chosen from vitamins C, B8, B3, B2, E, D and A, in particular from vitamins B8, B3 and D, and especially vitamins B8 and B3.

The daily doses that may be suitable for the invention are as follows: Vitamin C: 12 to 100 mg / day Vitamin B8: 7 to 100 mg / day Vitamin B3: 2 to 20 mg / day Vitamin B2: 0.2 to 5 mg / day Vitamin E: 2 to 15 mg / d Vitamin D: 0.5 to 50 mg / day Vitamin A: 100 to 900 mg / day Article N ° 432/2012 (EU regulation) dated 16 May 2012 legislates on health properties that can legally be declared on certain nutritional ingredients. More particularly in the field of the skin and its appendages, vitamin B8 is recognized to contribute to the maintenance of the skin. Water-soluble plant fibers According to a preferred embodiment, a water-soluble plant fiber suitable for the invention is glucomannan. Glucomannan Glucomannan is derived from an Amorphophallus konjac root fiber.

Konjac mannan, also known as glucomannan, is composed of a main chain of D-glucose residues linked by a link f3-1,4 to residues of D-mannose, and side chains composed of units 13-1. , 6 glucosyl. The degree of substitution by side chains is estimated at about 3 side chains for each chain of 32 units of sugar in the main chain. The side chains are composed of mannose and glucose units present in a molar ratio of 1.6: 1. Acetyl groups along the KGM backbone are present every 9 or 19 units of sugar. Acetyl groups contribute to the solubility properties of konjac mannan. Konjac mannan includes the compound having the following formula (I): (I) To practice the present invention, those skilled in the art may in particular use a konjac composition.

Konjac (Amorphophalus Konjac C. Koch) is a perennial plant belonging to the family Aracceae. The "Konnyaku" prepared from the konjac tuber has been used in traditional kitchens in Japan for several centuries. The main component of konnyaku is a glucomannan also called "konjac mannan" (or KGM). Konnyaku is prepared from konjac flour obtained from the dried konjac tuber. The KGM flour is obtained by grinding the tuber root of the plant of Amorphophalus Konjac C. Koch. With regard to Konjac, those skilled in the art can refer in particular to the review article by Chua et al. (2010, Journal of Ethnopharmacology, Vol 128: 268-278). Various techniques are known for obtaining a mannan composition from konjac flour. In a first technique, konjac flour is boiled in water and then treated with Fehling's liquor to convert the mannan to its complex with copper. The copper complex thus obtained is then decomposed to obtain the mannan, which is purified (see Agr Chem Soc Japan, 1930, Vol 6: 991-995). According to a second technique, the konjac flour is subjected to a water extraction step, and the impurities are removed by precipitation with ethanol. The precipitate is then subjected to several redissolution steps in the water before a drying step, after which a pure Konjac mannan composition is obtained (see Bull Chem Soc Japan, 1927, Vol 49: 298-322). . In a third technique, a konjac tuber grinder is subjected to one or more water extraction steps prior to separating the insoluble material, dialyzing the solid-free liquid against water, and then lyophilizing the material. dialysed liquid to remove water (see US Patent No. 3,973,008). Other methods for the preparation of konjac are 11 H 112011 (112011 H 0 H 0 0 H (H2) 11 H CY, C112011- 0 described in European Patent Application No. EP 2087798. Konjac flour having a capacity of rapid hydration is described in U.S. Patent No. 5,536,521, In general, mannan represents about 8 to 10% by weight, based on the total weight of the konjac tuber, for example, the mannan content of konjac flour is about 70% by weight, based on the total weight of flour.The use of glucomannan for weight reduction in the context of a nutritional regime has been validated by the European regulatory authorities (EFSA Claim 13.1) under strict conditions of use ie regular consumption for 1 month at a rate of 3 g glucomannan per day in 3 lg doses concomitant with the intake of l to 2 glasses of water Several clinical studies support this recommendation and all have a duration of use well above 3 weeks (4 to 6 weeks on average) to achieve a demonstrated clinical effectiveness. The glucomannan may advantageously be used according to the invention in a proportion of 3 g of glucomannan per day in 3 g taken for 5 to 14 days, and preferably for 7 days. Such a treatment duration constituting a sub-effective duration. Thus a use of glucomannan at 3 g per day for a period of time less than or equal to at most 2 weeks is an inefficient period of time to induce weight loss.

Probiotic microorganisms Probiotic microorganisms are microbial cell preparations or components of microbial cells that have a beneficial effect on the health or well-being of the host (Salminen S, Ouwehand A. Benno Y. et al, "Probiotics: How should they be defined" Trends Food Sci. 1999, 10: 107-10).

According to a preferred embodiment, a probiotic microorganism that is suitable for the invention may be Lactobacillus rhamnosus. Lactobacillus rhamnosus Lactobacillus rhamnosus is one of the probiotics isolated from the human microflora. The administration of Lactobacillus rhamnosus in obese mice has demonstrated a preventive action of weight gain for an administration period of 8 weeks (Lee 2006 - Human originated bacteria, Lactobacillus rhamnosus PL60, produce conjugated linoleic acid and anti-obesity show Biochimica and Biophysica Acta 1761 (2006) 736-744). WO 2009/021824 has also demonstrated an interest of Lactobacillus rhamnosus in the prevention of weight gain in an animal model after 8 weeks of use. In both studies, the use necessary for preventive action on weight gain (distinct action of a direct effect on weight loss) is at least 2 months to show significant clinical efficacy. Thus a use of Lactobacillus rhamnosus for a period of time less than or equal to at most one month is an inefficient period of time to induce weight loss. A Lactobacillus rhamnosus suitable for the invention may be chosen from Lactobacillus rhamnosus CGMCC 1.3724 or Lactobacillus rhamnosus CCN 4007. Lactobacillus rhamnosus may be used in inactivated live form. It can be grown and prepared for food shaping by any method known to those skilled in the art, or it can be obtained commercially from specialty suppliers such as Christian Hansen and Danisco in a form suitable for food processing. . A Lactobacillus rhamnosus can be used according to the invention at a rate of 1x107 cfu per day at 1x10 12 cfu per day for 4 to 7 weeks, and preferably for 5 to 6 weeks. Such a duration of treatment constituting a sub-effective duration in terms of weight loss. Prebiotic Compounds The term "prebiotic compound" is intended to mean food substances intended to promote the growth of probiotic bacteria in the intestine. A prebiotic compound suitable for the invention may be chosen from oligosaccharides, and optionally contain fructose, glucose, galactose, mannose, xylose, fructo-oligosaccharides and / or inulin, dietary fibers, or mixtures thereof. Inulin Fructan (13 (2-1) -linked fructose polymer) is naturally present in plants such as onions, asparagus, chicory, garlic, wheat or rye, and of general formula: (C6F11006) 11, with n superior or equal to 9.

The inulin suitable for the invention may be an inulin of plant origin extracted from chicory root (Orafti GRdu, supplier BeneOrafti). The literature shows that effective doses range from 4 to 10 g / day. According to the invention, the daily doses of inulin according to the invention are from 0.01 g / day to 1 g / day, preferentially from 0.03 to 0.8 g / day, for example for a duration of 4 to 7 weeks. These doses are called "under-effective". Fructo-oligo-saccharides (FOS) The fructose chains may or may not be terminated (in the case of inulin-derived oligofructoses) by a glucose unit. Fructo-oligosaccharides (FOS) belong to the group of fructans. They are natural polymers of D-fructose terminated by a molecule of D-glucose (linked to a molecule of D-fructose by a glycoside bond 1-1 '). The fructose molecules are linked together by f3 (1-2) osidic bonds. The number of associated fructose molecules is not greater than 10 in the case of FOS. FOS are complex molecules organized into linear or branched chains, usually firmly attached to other food compounds. The main dietary sources of FOS are vegetables (Jerusalem artichoke, onion, leek, chicory root, garlic, banana, asparagus), some cereals (barley, oats, rye ) and even mushrooms (shiitake for example). FOS are indigestible carbohydrates (fibers) and are fermented in the intestine by the colonic flora. Their presence in the intestine promotes the appearance of a flora consisting of lactobacilli and bifidobacteria, exerting a favorable effect in the host. The fermentation of FOS by the bacteria of the intestine also leads to the production of short-chain fatty acids (butyrate, acetate, etc.) which also have beneficial effects. These fermentability properties make FOS molecules of interest in the treatment and prevention of certain colonic (colon cancer, inflammatory diseases) and metabolic diseases (overweight, diabetes, hypercholesterolemia). In humans, several studies have shown that FOS consumed at 4 to 12.5 g / d induce an increase in the number of bifidobacteria in the stool. A more recent study on humans has even shown that doses ranging from 2.5 to 10 g / day are effective, the last dose being the maximum tolerable dose with no side effects. According to the invention, the daily doses of FOS are from 0.01 g / day to 1 g / day, preferably from 0.03 to 0.8 g / day, for example for a duration of 4 to 7 weeks. These doses are called under-effective.

The FOS that are suitable for the invention may be of commercial origin (Orafti 095, BeneOrafti supplier). Glucosamine and its derivatives Glucosamine is an amino sugar, especially of marine origin, composed of a glucose base and an amine function. It is a simple sugar of low molecular weight and is in the form of white crystals, soluble in water, of formula (1) or (2).

NI-12 OH OF IC OH Open Form (1) Cyclized Form (2) Glucosamine can be obtained from chitin which is a natural, complex, biodegradable sugar and is as naturally present as cellulose. In chitin, more than 60% of the total glucosamine is present in acetylated form. The chemical formula of chitin is: There is another source of chitin that can be used to produce glucosamine hydrochloride. It is chitin obtained from the biomass produced by fermentation of the A. figer fungus of the deuteromycetes class, order Monoliales, family Moniliaceae, genus Aspergillus and species figer.20 In the trade, glucosamine can be proposed under different forms of saline derivatives: sulfate, chloropotassic sulfate (2 KCl), hydrochloride (HC1), acetylated or polymerized (N-acetyl-glucosamine). The nature of the salt often depends on the process of obtaining used.

Thus, in the context of the present invention, it is understood that the term "glucosamine and its derivatives" includes all salified, acetylated and / or polymeric forms of glucosamine. Salified forms of glucosamine include sulfate, chloropotassium sulfate, chlorosodium sulfate and hydrochloride. An example of a method of production is given below by way of illustration.

Glucosamine in sulphated form can for example be prepared from shrimp shells (rich in chitin). In this process of obtaining, the first step consists of an acid hydrolysis in the presence of hydrochloric acid, carried out under vacuum, at 95 ° C. The hydrolysis conditions are dependent on the raw material.

Still in this process of obtaining, the reaction medium is then decolorized with activated carbon which retains the absorbable organic impurities. After gradual concentration in vacuo, the glucosamine hydrochloride obtained after hydrolysis crystallizes slowly in the cold for several hours. After filtration, it is washed with alcohol.

Finally, the last step of this method of obtaining is to neutralize glucosamine hydrochloride by potassium sulfate in aqueous phase. The mixture is then evaporated and the solid obtained dried under vacuum at 60 ° C. for several hours. When the chitin from the above fungus is used in place of shrimp shell chitin, it also undergoes hydrolysis to produce glucosamine. According to the invention, the daily doses of glucosamine can be 50 to 500 mg / day. Glucosamine can be administered, for example, for a period of 4 to 10 weeks. These doses and duration of administration are called "under-effective".

Plant Extracts A plant extract suitable for the invention may be selected from phenolic pine extracts or green tea extracts, or mixtures thereof. According to the invention, the daily doses of green tea extract can be from 50 mg to 400 mg / day. Daily doses of polyphenol pine extract can be 10 to 80 mg / day. A duration of administration of the plant extracts can vary from 4 to 10 weeks. These doses and duration of administration are called "under-effective". Phenolic extract of pine A pine extract suitable for the invention may be an extract of maritime pine bark (Pinus pinaster), and in particular of a maritime pine bark extract containing at least 60% by weight, of preferably at least 63% by weight of procyanidins. According to a first variant, it may be a maritime pine bark extract containing from 63 to 75% by weight of procyanidins, for example, the maritime pine extract marketed under the reference FLAVOPIN. by the company DRT. According to another variant, it may be a maritime pine bark extract containing at least 90% by weight, preferably 90 to 98% by weight of total polyphenols, for example in the form of extracts ( Maritime) Pine Bark of DRT. Within the meaning of the invention, the terms "procyanidin" and "proanthocyanidin" are equivalent and denote oligomers of flavonoids comprising, for example, catechin units. More particularly, the maritime pine bark extract considered according to the invention may contain procyanidics consisting of catechin and epicatechin units linked together by CC bonds so as to form dimers, trimers and other oligomers. chain length of up to 6 or even 7 molecules. The maritime pine bark extract considered according to the invention may also contain other compounds, and for example at least one compound chosen from catechins, epicatechins and organic acids, especially phenolic acids such as caffeic acid. , ferulic acid, p-coumarinic acid and p-benzoic acid. Of the above-mentioned organic acids, some may be in free form or in combination with glucose, and for example form, for example, glucose esters or glucose ethers. In particular, it is possible to use a maritime pine bark extract obtained according to the processes described in US Pat. No. 4,698,360, US Pat. No. 6,372,266 and US Pat. No. 5,720,956. By way of example of such extracts, mention may be made especially of the compound referred to under the name INCI Pinus pinaster (bark extract) and under the name CTFA Pine (Pinus pinaster) bark extract. It may in particular be the maritime pine extract marketed under the reference Flavopin by the company DRT (The Resin and Terpenic Derivatives).

According to the invention, daily doses of polyphenol pine extract can be from 10 to 80 mg / day. A duration of administration can vary from 4 to 10 weeks. These doses and duration of administration are called "under-effective". Green Tea Extract Green tea has a higher vitamin and polyphenol content than black tea because it has undergone little processing. The tea leaf is traditionally used orally in the symptomatic treatment of mild diarrhea, in functional asthenia, as an adjunct to dieting, or to promote the renal elimination of water (Source: Explanatory note from the Agency of the drug, 1998).

It contains at least 2% caffeine and 30% catechins. In recent years, green tea has attracted attention for the antioxidant properties of its phenolic constituents and its possible preventive role of the occurrence of cardiovascular or cancerous diseases. Polyphenols are natural antioxidants widely represented in plants. Unfermented green teas contain a remarkable 36% of total polyphenols in the dry weight of the leaves and thus constitute the richest food source (Trevisanato S. Tea and Health, 2000, Nutrition reviews 58, 1: 1 -10). Among other activities, tea is traditionally used as a drink and in large quantities to promote the renal elimination of water (Witchl M., Anton R.

Therapeutic plants, 2nd edition. TEC Publishing 2003: pp 605-606). Although present in a small amount in green tea (2%), caffeine gives it its mild stimulating action and diuretic. Finally, among the demonstrated physiological effects of tea catechins are vasodilator properties (Huang Y., Zhang A., Lau C.W., Chen Z.Y. (1998).

Vasorelaxant effects of purified green tea epicatechin derivatives in rat mesenteric artery. Life Sci; 63 (4): 275-283; Chen Z.Y., Law W.I., Yao X.Q., Lau C.W., Ho W.K., Huang Y. (2000). Inhibitory effects of purified green tea epicatechins on contraction and proliferation of arterial smooth muscle cells. Acta Pharmacol Sin .; 21 (9): 835-840), which could contribute to a positive effect on the microcirculation (contribution to a better elimination of cellular debris, and globules from destocking of fats). These effects are observed for very large intakes of catechins and caffeine: 150 mg of caffeine and 400 mg of catechins. In addition, caffeine has a diuretic activity for intakes of 250 to 350 mg.

According to the invention, the daily doses of green tea extract can be from 50 mg to 400 mg / day, ie 1 to 8 mg / day of caffeine and 15 to 120 mg / day of catechins. A duration of administration can vary from 4 to 10 weeks. These doses and duration of administration are called "under-effective".

L-arginine Arginine is an amino acid. It is one of the 20 main natural constitutive acids of proteins in its chiral L-arginine form. According to a preferred embodiment, the arginine selected in the context of the invention is of synthetic origin, from commercial source (supplier: Kyowa) and concentrated to 100%.

The literature shows that effective doses range from 4 to 10 g / day. According to the invention, the daily doses of arginine may be from 0.01 to 2 g / day, preferably from 0.03 to 1 g / day. A duration of administration can vary from 4 to 7 weeks. These doses and duration of administration are called "under-effective".

Combinations of active agents that are suitable for the invention may advantageously comprise at least two active agents that are distinct from one another and selected from a group comprising: vitamins C, B8, B3, B2, A, E, D ( in particular D3), glucomannan, Lactobacillus rhamnosus, copper, manganese, calcium, magnesium, iron, chromium, zinc, selenium, fibers, inulin, fructo-oligosaccharide, glucosamine, a phenolic pine extract, a green tea extract, or L-Arginine. According to another embodiment, a combination of actives of the invention may comprise glucomannan, glucomannan and vitamin B8; glucomannan and Lactobacillus rhamnosus; calcium, glucosamine, phenolic pine extract, and green tea extract; or vitamins B8, B3 and D, especially D3, a Lactobacillus rhamnosus, L-Arginine, a fructo-oligosaccharide, and inulin; Lactobacillus rhamnosus, vitamins B8 and B3, and fibers; Lactobacillus rhamnosus, vitamins B8 and B3 and a fructo-oligo-saccharide and / or inulin; Lactobacillus rhamnosus, vitamins B8 and B3 and fructo-oligo-saccharide and inulin. As indicated above, the invention can implement at least one, or even at least two, and preferably at least three combinations of at least two assets. A first combination of actives may comprise at least glucomannan, or glucomanane and / or vitamin B8, or glucomannan and / or lactobacillus rhamnosus. More preferably, a first combination may comprise at least, or consist of, glucomannan and vitamin B8, or glucomannan and Lactobacillus rhamnosus. Preferably, a first combination of assets comprises or consists of glucomannan.

A second combination of actives may comprise at least two active ingredients selected from vitamins B8 and B3, a Lactobacillus rhamnosus, and fibers. Also, a second combination of active agents may comprise at least two active agents selected from vitamins B8 and B3, a Lactobacillus rhamnosus, fructo-oligosaccharide, and inulin. Preferably, a second combination of active agents may comprise at least one vitamin B8 and at least one other asset defined above. Still more preferably, a second combination of active agents may comprise at least one vitamin B8 and at least one active agent selected from vitamin D, in particular D3, a Lactobacillus rhamnosus, L-Arginine, and fibers. More preferably, a second combination may comprise at least, or consist of, vitamins B8 and B3, Lactobacillus rhamnosus, vitamin D, especially D3, and L-Arginine. Preferably, a second combination of active ingredients comprises or consists of Lactobacillus rhamnosus, vitamin B8, vitamin B3, and fibers. More preferably, a second combination of actives comprises or consists of Lactobacillus rhamnosus, vitamin B8, vitamin B3, fructooligosaccharide, and / or inulin.

A third combination of actives may comprise at least two active ingredients selected from calcium, glucosamine, phenolic pine extract, and green tea extract. Preferably, a third combination of active agents may comprise at least calcium and at least one other asset defined above. Still more preferably, a third combination of actives may comprise at least calcium and at least one active ingredient selected from glucosamine, phenol pine extract, and green tea extract. Still more preferably, a third combination of actives may also comprise at least Lactobacillus rhamnosus and at least one active agent selected from arginine, vitamin D3, and fibers. More preferably, a third combination of actives may comprise at least, or consist of, calcium, glucosamine, phenol pine extract, and green tea extract. Alternatively, this third combination of actives may also comprise at least, or consist of, Lactobacillus rhamnosus, arginine, vitamin D3, and fibers. Preferably, a third combination of actives comprises or consists of calcium, green tea extract, phenol pine extract and glucosamine.

In a preferred manner, combinations of active agents that are suitable for the invention may advantageously be chosen from combinations comprising, or consisting of, at least glucomannan; vitamins B8 and B3, Lactobacillus rhamnosus, fibers; Lactobacillus rhamnosus, arginine and vitamin D3; Lactobacillus rhamnosus, vitamins B8 and B3 and fructo-oligo-saccharide and / or inulin; Lactobacillus rhamnosus, vitamins B8 and B3, fructo-oligo-saccharide, and inulin, calcium, glucosamine, phenolic pine extract, and green tea extract. In general, the active combinations of the invention are implemented so that the active agents are administered from 5 days to 1 month.

A first combination of actives of the invention may be administered for about 5 days. A second combination of actives of the invention may be administered for about 30 days. A third combination of actives of the invention may be administered for about 30 days. The active combinations of the invention are intended for the oral route and are advantageously formulated in the form of foods or food supplements. Such formulations may comprise an unmanageable carrier, the nature of which is adapted according to the type of composition considered. Examples of suitable foodstuffs are tablets, capsules or lozenges, suspensions, oral supplements in dry form and oral supplements in liquid form, milk, yogurt, cheese, fermented milks, fermented products. based on milk, ice cream, cereal-based products or fermented cereal-based products, milk-based powders, or confectionery-type foods, chocolate, cereals. The formulations of the invention may be made by any usual method known to those skilled in the art to produce oral solutions, dragees, capsules, gels, emulsions, swallowing or chewable tablets, capsules , in particular soft or hard capsules, dissolving granules, syrups, solid or liquid foods and hydrogels allowing a controlled release, food bars, powders, compacted or not, suspensions or liquid solutions, confectionery, fermented milk, fermented cheeses, chewing gums, toothpastes or spray solutions. The oral compositions may be in anhydrous form or in aqueous form according to the dermocosmetic indication.

An active ingredient or a combination of the invention may also be formulated with the excipients and components customary for such oral compositions or food supplements, namely in particular fatty and / or aqueous components, humectants, thickeners, agents and the like. preservatives, texture, flavor and / or coating agents, and / or antioxidants. The formulating agents and excipients for oral composition, and especially for food supplements, are known in the art and are not the subject of a detailed description here.

Methods A combination of active ingredients according to the invention is intended to be administered in addition to a dietary diet slimming. Advantageously, a use according to the invention may comprise a sequential administration of at least two combinations of at least two active ingredients, and preferably at least three combinations of at least two active ingredients in addition to a dietary slimming diet. The asset combinations considered may in particular be as defined above. A cosmetic method of the invention allows, simultaneously, (i) restore, maintain or promote a good aesthetic state of the skin and / or its appendages and (ii) promote weight loss during a diet slimming diet. A method of the invention comprises at least one step of administering to an individual in need, at least one combination of at least two nutritional assets capable of inducing weight loss, said active agents being used simultaneously, separated or sequential and being, respectively, administered at a dose and / or for a period of time unfit to induce weight loss. According to one embodiment, a method of the invention may comprise at least three successive periods of time consisting of: a first period of time comprising at least one administration of glucomannan or a first combination of at least two active agents comprising at least 30 glucomannan, - a second period of time comprising at least one administration of a second combination of at least two active agents comprising at least, or consisting of, a vitamin B8 or Lactobacillus rhamnosus, and - a third period of time comprising at least one administration of a third combination of at least two active agents comprising at least, or consist of, calcium or Lactobacillus rhamnosus. According to one embodiment, a method of the invention may comprise at least three successive periods of time consisting of: a first period of time comprising at least one administration of glucomannan or a first combination of at least two active agents comprising at least glucomannan; a second time period comprising at least one administration of a second combination of at least two active agents comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and fibers, and - a third period of time comprising at least one administration of a third combination of at least two active agents comprising at least Lactobacillus rhamnosus, vitamin D3 and Arginine, and fibers, or comprising at least or consist of a green tea extract, glucosamine, calcium and a phenolic pine extract. According to one embodiment, a method of the invention may comprise at least three successive periods of time consisting of: a first period of time comprising at least one administration of glucomannan or a first combination of at least two active ingredients comprising at least glucomannan; a second time period comprising at least one administration of a second combination of at least two active agents comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and fibers, and a third time period comprising at least one administration of a third combination of at least two actives comprising at least, or consist of, a green tea extract, glucosamine, calcium and an extract. phenolic pine. According to one embodiment, a method of the invention may comprise at least three successive time periods consisting of: a first period of time comprising at least one glucomannan administration or a first combination of at least two actives comprising at least glucomannan; a second time period comprising at least one administration of a second combination of at least two active agents comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and fructo-oligosaccharide, and / or inulin, and - a third period of time comprising at least one administration of a third combination of at least two active ingredients comprising, or consist of, at least one tea extract green, glucosamine, calcium and phenolic pine extract. According to one embodiment, a method of the invention may comprise at least three successive periods of time consisting of: a first period of time comprising at least one administration of glucomannan or a first combination of at least two active agents comprising at least 10 glucomannan; a second time period comprising at least one administration of a second combination of at least two active agents comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and fructo-oligosaccharide and inulin, and a third time period comprising at least one administration of a third combination of at least two actives comprising, or consist of, at least one green tea extract, glucosamine, calcium and phenolic pine extract. Advantageously, the first, second and third combinations of assets used above may correspond to the first, second and third combinations of assets previously defined. Preferably, a first combination of active agents used in a method of the invention may comprise at least, or consist of, glucomannan; glucomannan and vitamin B8; or glucomannan and Lactobacillus rhamnosus. Preferably, a second combination of active agents used in one method of the invention may comprise at least one vitamin B8 and at least one other asset defined above. More preferably, a second combination of active agents may comprise, or consist of, at least, or consist of, a vitamin B8 and at least one active ingredient selected from vitamin B3, Lactobacillus rhamnosus, fructo-oligosaccharide, and inulin. Alternatively, preferably, a second combination of actives may comprise, or consist of, at least one Lactobacillus rhamnosus, a vitamin B8, a vitamin B3, and fibers. More preferably, a second combination of actives may comprise, or consist of, at least one Lactobacillus rhamnosus, a vitamin B8, a vitamin B3 and a fructo-oligo-saccharide, and / or inulin. More preferably, a second combination of actives may comprise, or consist of, at least one Lactobacillus rhamnosus, a vitamin B8, a vitamin B3 and a fructo-oligosaccharide, and inulin. Preferably, a third combination of active agents used in a method of the invention may comprise at least calcium and at least one other asset defined above. Still more preferably, a third combination of actives may comprise at least, or consist of, calcium and at least one active agent selected from glucosamine, a phenolic pine extract, and a green tea extract. Alternatively, preferably, a third combination of active agents may comprise, or consist of, at least Lactobacillus rhamnosus, vitamin D3 and Arginine, and fibers, or comprise at least one green tea extract, glucosamine, calcium and a phenolic pine extract. Preferably a third combination of actives may comprise at least, or consist of, a green tea extract, glucosamine, calcium and a phenol pine extract. Preferably, a method of the invention may comprise a second period of time comprising at least one administration of a combination of active agents comprising, or consisting of, vitamins B8 and B3, a Lactobacillus rhamnosus, and fibers, and or a third period of time comprising at least one administration of a combination of active agents comprising, or consisting of, calcium, glucosamine, a phenolic pine extract, and a green tea extract.

Preferably, a method of the invention may comprise a second period of time comprising at least one administration of a combination of active agents comprising, or consisting of, vitamins B8 and B3, a Lactobacillus rhamnosus, fructooligo-saccharide, and / or inulin, and / or a third time period comprising at least one administration of a combination of active agents comprising, or consisting of, calcium, glucosamine, a phenol extract of pine, and an extract green tea. Preferably, a method of the invention may comprise a second period of time comprising at least one administration of a combination of active agents comprising, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and or a third period of time comprising at least one administration of a combination of active ingredients comprising, or consisting of, Lactobacillus rhamnosus, vitamin D3 and Arginine, and fibers, or comprising at least one tea extract green, glucosamine, calcium and phenolic pine extract. Still more preferably, a method of the invention may comprise a first period of time comprising at least one administration of a first combination of active agents comprising at least, or consisting of, glucomannan, or glucomannan and vitamin B8 or glucomannan and Lactobacillus rhamnosus, a second time period comprising at least one administration of a second combination of active agents comprising at least, or consisting of, vitamins B8 and B3, a Lactobacillus rhamnosus, and fibers, and a third time period comprising at least one administration of a third combination of active agents comprising at least, or consisting of, calcium, glucosamine, a phenolic pine extract, and a green tea extract. Still more preferably, a method of the invention may comprise a first period of time comprising at least one administration of a first combination of assets comprising at least, or consisting of, glucomannan, or glucomannan and vitamin B8 or glucomannan and Lactobacillus rhamnosus, a second period of time comprising at least one administration of a second combination of active ingredients comprising at least, or consisting of, vitamins B8 and B3, Lactobacillus rhamnosus, fructo-oligo-saccharide and / or inulin, and a third time period comprising at least one administration of a third combination of active agents comprising at least, or consisting of, calcium, glucosamine, a phenolic pine extract, and a green tea extract. Still more preferably, a method of the invention may comprise a first period of time comprising at least one administration of glucomannan or a first combination of at least two active agents comprising at least glucomannan, a second period of time comprising at least one least one administration of a second combination of active ingredients comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and a third time period comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin D3 and Arginine, and fibers, or comprising at least, or consisting of, green tea extract, glucosamine, calcium, and phenol pine extract. Still more preferably, a method of the invention may comprise a first period of time comprising at least one administration of glucomannan or a first combination of at least two active agents comprising at least glucomannan, a second period of time comprising at least one least one administration of a second combination of active ingredients comprising at least, or consisting of, Lactobacillus rhamnosus, vitamin B8, and vitamin B3, and fibers, and a third period of time comprising at least, or consisting of, green tea extract, glucosamine, calcium and phenolic pine extract. Generally, a first period of time may be of a duration ranging from 5 to 14 days, and preferably may be of a duration of 5 days. A second period of time may be of a duration ranging from 3 to 6 weeks, and preferably may be from 4 to 5 weeks, or about 30 days. A third period of time according to the invention may be of a duration varying from 3 to 6 weeks, and preferably may be from 4 to 5 weeks, or approximately 30 days. Sets The invention also relates to assemblies for implementing the methods and uses defined above. According to one embodiment, an assembly, in particular for implementing a method or a use of the invention, can comprise, conditioned separately, a first combination of active agents comprising at least, or consisting of, glucomannan; a second combination of active ingredients comprising at least, or consisting of, vitamins B8, B3, Lactobacillus rhamnosus, and fibers; and a third combination of active agents comprising at least, or consisting of, calcium, glucosamine, phenolic pine extract, and green tea extract. According to one embodiment, an assembly, in particular for implementing a method or a use of the invention, may comprise, conditioned separately, a first combination of active agents comprising at least, or consisting of, glucomannan and / or Lactobacillus rhamnosus; a second combination of active ingredients comprising at least, or consisting of, vitamins B8 and B3, Lactobacillus rhamnosus, fructo-oligosaccharide, and / or inulin; and a third combination of active agents comprising at least, or consisting of, calcium, glucosamine, phenolic pine extract, and green tea extract. According to one embodiment, an assembly, in particular for implementing a method or a use of the invention, can comprise, conditioned separately, a first combination of active agents comprising at least, or consisting of, glucomannan; a second combination of active ingredients comprising at least, or consisting of, vitamins B8 and B3, Lactobacillus rhamnosus, fructo-oligosaccharide and / or inulin; and a third combination of assets comprising at least, or consisting of, calcium, glucosamine, phenol pine extract, and green tea extract. According to one embodiment, an assembly, in particular for implementing a method or a use of the invention, can comprise, conditioned separately, a first combination of active agents comprising at least, or consisting of, glucomannan; a second combination of active ingredients comprising at least, or consisting of, vitamins B8 and B3, Lactobacillus rhamnosus, fructo-oligosaccharide, and inulin; and a third combination of assets comprising at least, or consisting of, calcium, glucosamine, phenol pine extract, and green tea extract. An assembly of the invention comprises a primary packaging, in which are packaged, each in a specific packaging, or secondary packaging, at least one combination of active ingredients of the invention as defined above. Thus, an assembly of the invention comprises a primary packaging, and three secondary packaging. Also, a set of the invention may furthermore comprise at least one written or figurative information, such as a diagram, on a support, for example a paper, plastic or electronic medium such as a USB key, and indicating the mode of use of the devices. combinations of assets. Such information may, for example, detail the dosage of combinations, the timing of their administration during the day, for example in the morning or in the evening, during or outside meals, the dose, the number of shots, single or fragmented in two or three doses, the mode of administration, for example simultaneous, separate or sequential, the duration of the treatment, for example from 5 days to 1 month, etc.

The following examples are presented by way of illustration of the embodiment of the invention, and should not be interpreted as limiting the scope of the teaching of the description or the claims. EXAMPLES Example 1 Sequential use of glucomannan, a combination of Lactobacillus rhamnosus, vitamin B8, vitamin B3 and fiber and a combination of calcium, green tea extract, phenol pine extract and glucosamine to achieve a loss of weight while promoting the beauty of the face and hair. Material & Methods The usage test lasts 65 days. It involves 180 women, aged 25 to 45 years, not premenopausal and wishing to lose between 1 and 8 kg.

It comprises three successive phases: 1 / "Attack" phase (first period): quantitative study performed after 5 days of use of the attack phase (glucomannan). 2 / "Slimming" phase (second period, as part of a low calorie diet): quantitative study carried out after 30 days of use of the Slimming Phase (combination of Lactobacillus rhamnosus, vitamin B8, vitamin B3, and fiber ) 3 / "Stabilization" phase: a quantitative study carried out after 30 days of use of the Stabilization Phase (combination of calcium, green tea extract, phenolic pine extract and glucosamine) Result A progressive loss of weight is observed throughout the program: 1 / At the end of period 1 or "Attack phase": - 91% of women lost from 1 to 3 kg - 9% of women did not lose weight 2 / At the end of period 2 or "slimming phase" - 54% of women lost from 1 to 3 kg - 39% of women lost from 4 to 6 kg - 6% of women lost 7 kg or more - 1% of women did not lose weight 3 / Over the entire program - 47% of women lost 4 to 6 kg - 40% women lost 1 to 3 kg - 13% of women lost 7 kg or more30

Claims (11)

REVENDICATIONS1. Cosmetic use, during a weight loss diet, of a combination of at least a first and a second nutritional asset capable of inducing weight loss to (i) restore, maintain or promote a good aesthetic state of the skin and and / or its annexes, and (ii) promote weight loss, said active agents being carried out simultaneously, separately or sequentially and being, respectively, administered at a dose and / or for a period of time unfit to induce a loss of weight. weightloss. 2. Use according to claim 1, for restoring, maintaining or promoting a synthesis of collagen or connective tissue, to protect the skin cells against oxidative stress, to promote the quality and growth of the hair, to help reduce the fall hair, to restore, maintain or promote the normal development of the skin, mucous membranes, nails or hair, to restore, maintain or promote the beauty of the face or hair, to restore, maintain or promote normal pigmentation of the skin skin or hair, or to restore, maintain or promote normal function of the skin, mucous membranes, nails or hair. 3. Use according to claims 1 or 2, wherein the active ingredients are selected from vitamins, water-soluble or insoluble vegetable fibers, probiotic microorganisms, minerals, prebiotic compounds, glucosamine and its derivatives, plant extracts, or amino acids. 4. Use according to any one of the preceding claims, wherein the active ingredients are selected from vitamins C, B8, B3, B2, A, E, D, glucomannan, Lactobacillus rhamnosus, copper, manganese, calcium. , magnesium, iron, chromium, zinc, selenium, fiber, inulin, fructo-oligosaccharides, glucosamine, phenolic pine extract, green tea extract, or L-Arginine. Use according to any one of the preceding claims, wherein the combination of active ingredients comprises glucomannan, glucomannan and vitamin B8; glucomannan and Lactobacillus rhamnosus; calcium, glucosamine, phenolic pine extract, and green tea extract; or vitamins B8, B3 and D, especially D3, Lactobacillus rhamnosus, L-Arginine, fructooligosaccharide, and inulin; Lactobacillus rhamnosus, vitamins B8 and B3, and fibers; Lactobacillus rhamnosus, vitamins B8 and B3 and a fructo-oligo-saccharide and / or inulin; Lactobacillus rhamnosus, vitamins B8 and B3 and fructo-oligo-saccharide and inulin. Use according to any one of the preceding claims, comprising sequentially administering at least a first and a second combination of assets, and preferably at least a first, a second and a third combination of assets. 7. Cosmetic method for (i) restoring, maintaining or promoting a good aesthetic state of the skin and hair and / or their appendages and (ii) promoting weight loss during a dieting diet, said method comprising at least one step of administering, to an individual in need thereof, at least one combination of at least a first and a second nutritional asset capable of inducing weight loss, said active agents being used simultaneously, separately or in combination with each other; sequential and being, respectively, administered at a dose and / or for a period of time unfit to induce weight loss. 8. Method according to the preceding claim, wherein said assets are as defined in claims 3 to 5. The method according to claim 7 or 8, said method comprising at least three successive periods of time consisting of: a first period of time comprising at least one administration of glucomannan or a first combination of at least two active ingredients comprising at least less glucomannan; - a second period of time comprising at least one administration of a second combination of active agents comprising at least one Lactobacillus rhamnosus, a vitamin B8, and a vitamin B3, and fibers, and - a third period of time comprising at least one administration of a third combination of active agents comprising at least Lactobacillus rhamnosus, vitamin D3 and Arginine, and fibers, or comprising at least one green tea extract, glucosamine, calcium and a phenolic pine extract. 10. Method according to the preceding claim wherein said second period of time comprises at least one administration of a combination of active agents comprising vitamins B8 and B3, a Lactobacillus rhamnosus, and fibers, and / or said third period of time comprises at least one administration of an active combination comprising calcium, glucosamine, phenol extract of pine, and green tea extract. 11. A combination for carrying out a method as defined in claims 7 to 10 comprising, conditioned separately, a first combination of actives comprising at least glucomannan; a second combination of active ingredients comprising at least vitamins B8 and B3, Lactobacillus rhamnosus, and fibers; and a third combination of actives comprising at least calcium, glucosamine, phenol pine extract, and green tea extract.