FR2964875A1 - Antioxidant composition, useful as functional food/cosmetic product, to prevent premature aging of cells in an organism, comprises an extract of aerial parts of nettle, an Ascophyllum thalli extract, acerola extract and ascorbic acid - Google Patents

Abstract

Antioxidant composition comprises at least one extract of aerial parts of nettle, an extract of Ascophyllum thalli, an extract of acerola and ascorbic acid. Independent claims are included for: (1) functional food comprising the composition; (2) #a cosmetic product comprising the antioxidant composition; (3) #a preparation of the composition comprising carrying a polar solvent extraction from the extracts of aerial parts of nettle, Ascophyllum thalli, and fruit of Malpighia punicifolia, to obtain a nettle extract comprising 0.05-50 wt.% of polyphenols, extract of Ascophyllumcontaining 1-70 wt.% of polyphenols, and acerola extract containing 1-80 wt.% of vitamin C. ACTIVITY : None given. MECHANISM OF ACTION : None given.

Description

The present invention relates to an anti-oxidant composition based on aerial part of nettle (Urtica dioica), ascophyllous nodosa thallus extract (Ascophyllum nodosum), acerola extract (Malpighia punicifolia) and vitamin C (ascorbic acid) having an antioxidant effect to contribute to the protection of cellular constituents against oxidative damage. An object of the present invention relates to a food or a cosmetic product comprising the aforementioned composition, as well as their uses.

The present invention also relates to the process for preparing the antioxidant composition. Oxidative stress is the imbalance between the production of free radicals and the antioxidant defenses. Under normal conditions, free radicals are produced continuously in our body and are destroyed by antioxidant defenses. This process is harmful to the body and is an important cause of damage to cell structures including lipids and membranes, proteins and DNA. Free radicals come from many sources both extra and intracellular. Among the extracellular sources, it is possible to distinguish several types of phenomena inducing free radicals, such as physical sources (ultraviolet radiation, radiation etc ...), chemical sources (pesticides, various atmospheric pollution, food pollutants, drugs, tobacco smoke etc ...). Intracellular sources include oxygen metabolism (mitochondrial respiratory chain), the immune system (production of free radicals to lyse pathogens), or prolonged physiological stress (physical or mental effort).

Exposure to free radicals from various sources (neutrophils, UV radiation, air and food pollutants, metabolism ...) has led the body to develop a series of defense mechanisms. Mechanisms of defense against free radicals induced by oxidative stress relate to: (i) mechanisms of prevention, (ii) mechanisms of repair, (iii) physical defenses, and (iv) antioxidant defenses. Enzymatic antioxidant defenses include superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT). The main non-enzymatic antioxidants are ascorbic acid (vitamin C), a-tocopherol (vitamin E), glutathione (GSH), carotenoids, flavonoids and other polyphenols. Antioxidants, synthesized by the body or brought by the diet or by cosmetics, are therefore necessary for homeostasis of the body to avoid an imbalance of free radicals causing serious cell damage and dysfunction, such as aging , inflammatory diseases etc. The invention thus aims to provide an antioxidant composition at least as effective as those existing on the market at present.

For this purpose, the present invention relates to an antioxidant composition comprising at least one aerial nettle extract, an ascophyllum thallus extract, an acerola extract and ascorbic acid. By extract is meant a composition obtained from a plant by a physical and / or chemical operation, in particular at least a part of the plant with at least one solvent. The present applicant has surprisingly discovered that the combination of an extract of aerial part of nettle (Urtica dioica), an extract of thallus of ascophyllous nodosa (Ascophyllum nodosum) with an extract of acerola ( Malpighia punicifolia) and vitamin C (ascorbic acid), has an antioxidant activity greater than that corresponding to the sum of the activities of each of the components of this composition. This allows to reduce the amount of free radicals, increase the antioxidant defenses, reduce the cellular damage (protein, lipids, DNA) related to oxidative stress.

The large nettle (Urtica dioica L.), also known as stinging nettle or common nettle, is a herbaceous, perennial plant of the family Urticaceae and the genus Urtica. Stinging nettle consists mainly of polyphenols, polysaccharides and minerals (quercetin glucosides and rutinosides, kaempferol and isorhamnetin, caffeoylmalic acid, chlorogenic acid, neochlorogenic acid, caffeic acid, scopoletin (cumarine), sitosterol (-3-O-glucoside)) . This plant is generally used for applications: food (salad, vegetable) both in humans and animals, agricultural (nettle leaf slurry for killing or repelling insects and as a fertilizer), industrial (widely used fiber) to make ropes, threads and clothes), or medicinal. Regarding the medicinal uses of nettle, many studies have shown the benefit of its root for the prostate, particularly in the case of benign hyperplasia. On the other hand, the use of the aerial parts of the plant is supported by a smaller number of studies. The leaves are used in particular for a wide variety of applications as a diuretic, for osteoarthritis, prostatitis, rheumatism, rheumatoid arthritis, high blood pressure or allergic rhinitis. Ascophyllum nodosum, or Ascophylle nodosa, is a large brown algae of the family Fucaceae. Ascophyllum is usually grown or harvested for its alginates, for fertilizer and for the production of animal and human food. Ascophyllum contains fucans as well as polyphenols consisting of phloroglucinol unit and having antioxidant activity. The antioxidant effect of an ascophyllum extract is correlated with the content of phenolic compounds in thalli. Malpighia punicifolia synonym of Malpighia glabra, or Acerola, is a tree of the Malpighiaceae family whose berry is used for its vitamin C content. Vitamin C (or L-ascorbic acid) is a water-soluble vitamin. It can be provided by various sources, the best known being Acerola (Malpighia punicifolia), Cynnorhodon (Rosa canina), Ortho siphon (Orthosiphon stamineus, O. aristatus, O. spicatus), Camu camu (Myrciaria dubia) ), guava (Psidium guava), Kiwi (Actinidia chinensis) or fruits of the Citrus genus.

This vitamin, which plays a major role in protecting the body's cells against damage caused by free radicals, is essential for the metabolic functioning of the body. As an antioxidant, vitamin C traps free radicals such as reactive oxygen species (superoxide anion, hydroperoxyl radical, peroxyl radical, singlet oxygen, ozone) and nitrogen (peroxynitrite, nitrogen dioxide, radical nitroxide), thus protecting other substrates, such as proteins, lipids and DNA from oxidative damage. Vitamin C can be taken orally or topically. Thus, several studies have shown that ascorbic acid provided as a cosmetic product has anti-aging effects on the skin. Preferably, the antioxidant composition comprises, by weight, relative to its total weight, 0.1% to 85% extract of aerial nettle parts, 0.1% to 85% of ascophyllum extract 0.1% to 85% acerola extract and 0.1% to 85% ascorbic acid.

Even more preferably, the antioxidant composition comprises, by weight, based on its total weight, 30% to 70% nettle extract, 05% to 25% ascophyllum extract, 5% to 15% by weight. % acerola extract and 30% to 70% ascorbic acid. An object of the present invention also relates to a food or cosmetic product comprising said antioxidant composition as described above. A food is understood to mean a food combining the notion of food and medicine as part of a functional diet. This food may be under the form of a food supplement or a fortified food A dietary supplement is by definition a foodstuff whose purpose is to supplement a normal diet and which constitutes a concentrated source of nutrients or other substances having a nutritional value. nutritional or physiological effect, alone or in combination, marketed in the form of doses. By dose is meant all conventional forms: capsules, lozenges, tablets, ampoules, or sachets. Foodstuffs intended for particular nutritional uses are foodstuffs which, by reason of their particular composition or particular process of manufacture, are clearly distinguishable from foodstuffs for everyday consumption, are suitable for the nutritional purpose indicated and are Such fortified foods are, by definition, products which have been supplemented with nutrients, such as yogurts or beverages, in which the antioxidant composition has been dissolved according to the invention. the invention.

A cosmetic is a substance or preparation intended to be brought into contact with various superficial parts of the human body, in particular the epidermis, the hair and hair systems, the nails, the lips, the external genital organs, the teeth and the oral mucosa. , in view, exclusively or mainly, to clean, protect, perfume, maintain in good condition, change their appearance or smell. Preferably, the food or cosmetic product comprises, by weight, relative to the total weight of said food or said cosmetic product, from 0.1% to 85% of said antioxidant composition. Even more preferably, the food or cosmetic product comprises, by weight, relative to the total weight of said food or said cosmetic product, from 3.0% to 65% of said antioxidant composition.

Advantageously, the food or cosmetic product further comprises at least one of the active agents or extracts chosen from: vitamins such as vitamin E; carotenoids such as beta-carotene and lutein; coenzyme Q10; alpha lipoic acid; trace elements / minerals such as selenium; rosemary extract; thyme extract; melissa extract; basil extract; tea extract; berry extract; orthosiphon extract,; camu camu extract; Orthosiphon extract, polyphenols, or a mixture thereof. Other pharmaceutically acceptable agents and / or food and / or cosmetic can be added to the composition, such as fillers, fluidizers, natural extracts, vitamins, minerals, trace elements, amino acids , fatty acids, anti-caking agents, natural oils, flavors, colorants, acidifiers, thickeners, preservatives, sweeteners, adjuvants, colorants, emulsifiers, surfactants, viscosity. The fillers are advantageously microcrystalline cellulose, maltodextrin potato or corn. The thickener used preferably is potato starch, hydroxypropyl methylcellulose, citrus pectin, guar gum, carob, agar-agar, konjac, hydrogenated oils, beeswax.

The fluidizers may be magnesium silicate, magnesium stearate, colloidal silica. Anti-caking agents are those usually used in the food industry, such as magnesium stearate, colloidal silica. Vitamins are selected from, among others, vitamins E, B6, B1, B2, B3, B5, B8. As an acid, citric acid may be used in the composition of the present invention.

The stabilizers employed in the manufacture of said composition are those usually used in the food and cosmetic industries, derived from sugar and cereals, such as sorbitol. The aromas that can be used are varied, such as aroma of coffee, lemon, apple, peach, chocolate, vanilla, strawberry.

The sweeteners used are, inter alia, inulin, xylitol, aspartame, glucose syrup, fructo-oligosaccharide syrup, maltitol powder or syrup, acesulfame potassium, fructooligosaccharide, sucralose, neohesperidin, steviosides and sodium cyclamate.

Fatty acids can also be added to the composition, such as omega 3, omega 6, galactolipids, but also minerals: chromium, boron, magnesium, calcium, iron, molybdenum and amino acids, such as tryptophan, leucine, arginine and glycine. Preservatives are useful so that said composition is preserved over time. The preservatives used may be, for example, potassium sorbate, parabens, sodium benzoate, citric acid, benzoic acid, rosemary or ascorbyl palmitate (antioxidant). The surfactants make it possible to solubilize two non-miscible phases. The surfactants used may be sugar derivatives such as cetearyl glucoside, lauryl glucoside or coco glucoside. All of these compounds are in no way limiting, pharmaceutically and / or food and / or cosmetically acceptable agents that can be added to the composition according to the present invention.

Preferably, the food or cosmetic product is in the form of a solution or an aqueous suspension in a glass ampoule, stick or drink, dropper bottle, spray, or in the dry state of tablet coated or uncoated, capsules, dragees, capsules, powder, effervescent tablets, granules, sticks, pellets, and wherein the cosmetic product is in the form of cream, lotion, care, shampoo , soap, patch, gel or paste. Preferably, the food or cosmetic product, as described above, is applied to prevent the premature aging of cells in an organism and / or protect it against damage caused by free radicals and oxidative stress. The present invention also relates to the use of a food or cosmetic product, as described above, wherein the food is administered to a human one to four times a day, per dose comprising from 10 mg to 3 g, preferably from 20 mg to 2 g and even more advantageously from 100 mg to 900 mg of said antioxidant composition, and the cosmetic product is applied to a human, one to four times a day, per dose comprising from 10 mg to 30 g, preferably from 20 mg to 20 g of the antioxidant composition and this, for a period of 5 days to 3 years. The antioxidant effect of the present invention is beneficial for skin, hair, joint, cardiovascular, digestion, tonus, thinness, cerebral, vision, bone, sports, cellular aging and more broadly to all cellular structures sensitive to free radicals and oxidative stress. Another object of the present invention relates to a process for the manufacture of the aforementioned antioxidant composition, characterized in that it comprises the step of: - extracting with a polar solvent from extracts of: aerial parts of nettle extract, ascophyllum thallus, acerola extract, and Malpighia punicifolia fruit, to obtain a nettle extract comprising from 0.05% to 50% by weight of polyphenols total, based on the total weight of said nettle extract obtained, an ascophyllum extract comprising from 1% to 70% by weight of total polyphenols, relative to the total weight of said ascophyllum extract obtained, and an acerola extract comprising from 1% to 80% by weight of vitamin C, relative to the total weight of said acerola extract obtained. The various constituents of the antioxidant composition can be provided individually or together in the final product (food, cosmetics) according to the manufacturing facilities. This process may comprise more particularly the following steps: nettle in the form of a leaf is placed respectively in the form of thalle-like ascophyllum and berry-shaped acerola in a polar solvent. , then an extraction step is performed and the extracts obtained in the previous step are respectively recovered. The polar solvent is preferably a solvent having a dipole moment other than zero and in particular greater than 1.5. The polar solvent is advantageously a protic solvent. Alcohols such as methanol, ethanol, propanol, butanol and pentanol, water such as osmosis water, distilled and permuted, as well as one of their mixtures, are suitable as polar solvents according to the present invention.

The solvent may furthermore comprise an additive, such as an antioxidant, a preservative, one or more enzymes, in particular intended to degrade the plant walls, for example to facilitate the extraction, an anti-foaming agent, an acid or still a basis, especially to modify the isoelectric point and promote precipitation. The extraction step can be done by: decoction, maceration, infusion, digestion, percolation, leaching, hot water entrainment, such as distillation or hydrodistillation, microwave assisted solvent, and ion exchange column .

The decoction comprises the following steps: - the boiling of a mixture comprising nettle leaves or ascophyllum thalli or acerola berries in a polar solvent as defined above; and maintaining this boiling for a particular period, in particular from 1 minute to 2 hours, in particular approximately 20 minutes, then possibly returning to ambient temperature.

By "maceration" is meant a method of cold soaking nettle leaves or ascophyllum thalli or acerola berries in the polar solvent for a particular period of time in order to extract the soluble compounds therefrom. In particular, the maceration step has a duration ranging from 1 hour to 15 days, and preferably 1 day. In particular, it is at room temperature, ie 10 to 25 ° C. The infusion is a process of pouring the polar solvent preferably at boiling temperature onto nettle leaves or ascophyllum thalli or acerola berries and then allowing the infusion mixture for a period of from one minute to two hours. Digestion is a method of heating a mixture comprising nettle leaves or ascophyllum thalli or acerola berries in the polar solvent at a temperature below the boiling temperature, for example in the case of water at 10 to 89 ° C, and in particular 25 to 70 ° C. By "percolation" or "leaching" is meant a process of passing the polar solvent through nettle leaves or ascophyllum thalli or acerola berries.

The distillation can be carried out for example by VMHD (pulsed vacuum microwave hydrodistillation). During this extraction step, the polar solvent is brought to a pressure greater than atmospheric pressure. In particular when the solvent is water, it may be in the subcritical state, that is to say that the water is at a temperature between 100 and 374 ° C and at a pressure between 1 and 218 bars. Under these conditions, the water is in liquid form. At the end of the extraction step, the polar solvent is brought to a temperature ranging from ambient temperature (25 ° C.) to a temperature of 300 ° C. above the boiling point of the solvent used. and preferably at the boiling point of the solvent used. Thus, the polar solvent is at least partially reduced or completely eliminated, in particular by evaporation, by sublimation, and preferably under reduced pressure. The term "boiling point of the solvent" means the highest temperature that the solvent can reach before evaporating, in gaseous form, freely. This temperature is calculated at atmospheric pressure of 1 atmosphere (101.3 kPa). The extraction step of the process according to the invention may be continuous or discontinuous.

The method according to the invention may comprise a step involving heating, in particular of a duration ranging from 1 second to 15 days, and in particular from 10 seconds to 8 hours. In an additional step, the extracts recovered after extraction can be sterilized, in particular by heating with or without pressure and / or by passage over a membrane. These extracts of nettle leaves, ascophyllum thalli and acerola berries can also be disinfected, in particular by heating with or without pressure or by means of a disinfecting agent. Finally, these extracts are generally dried. This drying can be carried out by lyophilization and / or by atomization. This polar solvent extraction process thus makes it possible to obtain extracts of nettle leaves, thalli of ascophyllum, acerola berries respectively sources of polyphenols and vitamin C. In fact, the extract of nettle obtained comprises from 0.05% to 50% of total polyphenols, and especially from 0.10% to 10%. The ascophyllum extract obtained comprises from 1% to 70% of total polyphenols, and especially from 2% to 20%. The acerola extract obtained comprises from 1% to 80% of vitamin C, and especially from 2% to 50%. However, as will be explained below, the anti-oxidant composition, combining a nettle extract, an ascophyllum extract, an acerola extract and ascorbic acid, present, and this surprisingly an antioxidant activity greater than that corresponding to the sum of the activities of each of the components of this composition expected by those skilled in the art.

Examples A to F of compositions for food (food supplement or fortified food), as well as examples G and H of cosmetic product, are given as an illustration: 3510 Example A: Ingredients Per stick in mg For 100 g in g Nettle extract 40.00 2.00 Ascophyllum extract 15.00 0.75 Acerola extract 20.00 1.00 Ascorbic acid 5.00 0.25 Fructo oligosaccharides 1200.00 60.00 Aroma 10.00 0.50 Sweetener 5.00 0.25 A food supplement or fortified food is obtained in the form of a powder, then packaged in a bag (stick). Said dietary supplement or fortified food is able to be diluted in a glass of water or it can be ingested directly by humans.

Example B: Ingredients Per jar in mg For 100 g in g Nettle extract 80.00 3.04 Ascophyllum extract 30.00 1.14 Acerola extract 40.00 1.52 Ascorbic acid 10.00 0, 38 Inulin 1820.00 68.94 Flavor 25.00 0.95 Sweetener 5.00 0.19 Dyestuff 10.00 0.3 8 Maltodextrin qs qs 100 g Fortified food or fortified food is packaged as a cork adapted to be screwed on a bottle 11 of water of 300 mL, 1 L or 1.5 L. in order to use this supplement or this fortified food, it will be sufficient for the user to screw the stopper containing said composition on a bottle 300 mL, 1 L or 1.5 L containing, for example, water. The recommended dose for the individual will then be 1 cork per day.

Example C Ingredients per ampoule in mg Per 100 g in g Nettle extract 80, 00 0, 80 Ascophyllum extract 60.00 0.60 Acerola extract 40.00 0.40 Ascorbic acid 20.00 0, Aroma 50.00 0.50 Sweetener 10.00 0.10 Water qs qs 100 g After autoclaving, a composition consisting of extracts of nettle, ascophyllum and acerola according to the invention is obtained. in a light bulb. The recommended dose is one ampoule per day to be diluted in a glass of water.

Example D: Ingredients Per jar in mg For 100 g in g Nettle extract 18000.00 11.25 Ascophyllum extract 14000.00 8.75 Acerola extract 9000.00 5.63 Ascorbic acid 7000.00 4, Inulin 112 000.00 70.00 5 A food supplement or fortified food is obtained in the form of extruded granules. The recommended daily dose is 2.6 g of granules, dosed for example with a teaspoon and diluted in a glass of water.

Example E: Ingredients Per capsule in mg For 100 g in g Nettle extract 95.00 24.7 Ascophyllum extract 65.00 16.9 Acerola extract 25.00 6.5 Ascorbic acid 10.00 2, 6 Excipients: 1.00 0.26 Colloidal silica QS 1.00 0.26 Magnesium stearate QS The recommended daily dose is 1 capsule per day. Example F: Ingredients Per vial in mg For 100 g in g Nettle extract 7500.00 1.50 Ascophyllum extract 4000.00 0.80 Acerola extract 3100.00 0.62 Ascorbic acid 1000.00 0, 20 Flavor 4000.00 0.80 Sweetener 500.00 0.10 Citric acid pH <4.5 500.00 0.10 Potassium sorbate 500.00 0.10 Sodium benzoate 500.00 0.105 The dietary supplement or the fortified food according to this example is packaged in a bottle with measuring cup. The recommended daily dose is 25 ml (to be metered with the measuring cup) and diluted in a glass of water.

Example G: Ingredients For 100 g in g Water QSP 100.00 Caprylic / capric triglyceride 12.00 Cetearyl alcohol 8.50 Coco-caprylate / Caprate 2.70 Glycerin 2.40 Cera alba 1.70 Cetearyl glucoside 1.00 Extract Ascophyllum 0.120 Sodium dehydroacetate 0.0644 Nettle extract 0.045 Benzyl alcohol 0.80 Mel 0.80 Perfume 0.50 Simmondsia chinensis oil 0.20 Acerola extract 0.07 Maltodextrin 0.03 Xanthan gum 0, 10 Tocopherol 0.10 Helianthus root oil 0.09 annuus Citric acid 0.02 Linalool 0.03012 Lemongrass oil 0.01502 Limonene 0.011705 The cream is spread on the face every night. Example H: Ingredients For 100 g in g Water QSP 100.00 Triglyceride 5.00 caprylic / capric Cetearyl alcohol 4.40 Glycerin 1.40 Cetearyl glucoside 0.60 Ascophyllum extract 0.12 Nettle extract 0.045 Sodium dehydroacetate 0.014 Benzyl alcohol 0.90 Perfume 0.50 Butyrospermum butter 0.40 parkii Xanthan gum 0.30 Acerola extract 0.07 Maltodextrin 0.03 Sodium dehydroacetate 0.10 Citric acid 0.012 Linalool 0.03012 Lemongrass oil 0.01502 Limonene 0.0117 A milk is spread on the body one to several times a day.

The present applicant has demonstrated the antioxidant activity of a composition comprising a nettle extract, an ascophyllum extract, an acerola extract and ascorbic acid, by an analysis of the antioxidant capacity by the ORAC method ( Oxygen Radical Absorbance Capacity). The ORAC method allows a global measurement of the antioxidant power of pure or mixed substances as in finished products. The first step consists in extracting the active ingredients in a mixture of water and ethanol for the water-soluble molecules and in ethanol for the lipophilic ones. The test is carried out in spectrofluorimetry. It consists of a measurement of the protection exerted by a given molecule against the oxidation of fluorescein by a stable free radical, AAPH (2,2-azobis- (dimethylaminopropane hydrochloride). The results are expressed in relation to the protection exerted by a reference antioxidant, Trolox®, which is the water-soluble equivalent of vitamin E and reduced by gram of the test product. ORAC test is to propose a standardized and widely accepted measure of antioxidant power, contrary to the many other existing methods Sample Results (μmoles of trolox® equivalents / g of product) Extract of aerial parts of nettles 1600 Extract of ascophyllum nodosum 1090 Acerola berry extract 400 L-ascorbic acid 2660 Antioxidant composition 616020 In addition, this composition has an antioxidant activity greater than that of c corresponding to the sum of the activities of each component of this composition.

Although the invention has been described in connection with a particular embodiment, it is obvious that it is not limited thereto and that it comprises all the technical equivalents of the means described and their combinations if they are within the scope of the invention

Claims (17)

REVENDICATIONS1. Antioxidant composition comprising at least one aerial nettle extract, an ascophyllum thallus extract, an acerola extract and ascorbic acid. 2. An antioxidant composition according to claim 1, comprising, by weight, relative to the total weight of said composition, 0.1% o to 85% extract of aerial parts of nettle, 0.1% to 85% d ascophyllum extract, 0.1% to 85% acerola extract and 0.1% to 85% ascorbic acid. 3. Antioxidant composition according to claim 2, comprising, by weight, relative to the total weight of said composition, 30% to 70% of nettle extract, 05% to 25% of ascophyllum extract, 5% 15% acerola extract and 30% to 70% ascorbic acid. 15 4. A plant comprising said antioxidant composition according to one of claims 1 to 3. 5. The plant according to claim 4, comprising, by weight, relative to the total weight of said medicament, from 0.1% to 85% of said antioxidant composition. 20 6. An animal according to claim 5, comprising, by weight, based on the total weight of said food, from 3.0% to 65% of said antioxidant composition. The plant of any one of claims 4 to 6, further comprising at least one of the active agents or extracts selected from vitamins such as vitamin E; carotenoids such as beta-carotene and lutein; coenzyme Q10; alpha lipoic acid; trace elements / minerals such as selenium; rosemary extract; thyme extract; melissa extract; basil extract; tea extract; berry extract; orthosiphon extract,; camu camu extract; Orthosiphon extract, polyphenols, or a mixture thereof. 8. The animal according to one of claims 4 to 7, wherein the food is in the form of a solution or an aqueous suspension in a glass ampoule, stick or drink, dropper bottle, in spray, or in the dry state of naked or coated tablets, capsules, lozenges, capsules, powder, effervescent tablets, granules, sticks, lozenges. 9. An animal according to one of claims 4 to 8, applied to prevent the premature aging of cells in an organism and / or protect it against damage caused by free radicals and oxidative stress. 10. Cosmetic product comprising said antioxidant composition according to one of claims 1 to 3. 11. Cosmetic product according to claim 10, comprising, by weight, relative to the total weight of said cosmetic product, from 0.1% to 85% of said antioxidant composition. 12. Cosmetic product according to claim 11, comprising, by weight, relative to the total weight of said cosmetic product, from 3.0% to 65% of said antioxidant composition. 13. Cosmetic product according to one of claims 10 to 12, further comprising at least one of the active agents or extracts chosen from: vitamins such as vitamin E; carotenoids such as beta-carotene and lutein; coenzyme Q10; alpha lipoic acid; trace elements / minerals such as selenium; rosemary extract; thyme extract; melissa extract; basil extract; tea extract; berry extract; orthosiphon extract,; camu camu extract; Orthosiphon extract, polyphenols, or a mixture thereof. 14. Cosmetic product according to one of claims 10 to 13, wherein the cosmetic product is in the form of cream, lotion, care, shampoo, soap, patch, gel or paste. 15. Cosmetic product according to one of claims 10 to 14, applied to prevent the premature aging of cells in an organism and / or protect it against damage caused by free radicals and oxidative stress. 16. Use of a cosmetic product according to claim 15, characterized in that it is applied to a human, one to four times a day, per dose comprising from 10 mg to 30 g of the antioxidant composition during a period of 5 days to 3 years. 17. A method of manufacturing the antioxidant composition according to one of claims 1 to 3, characterized in that it comprises the step of: - extracting with a polar solvent from extracts: parts aerial extracts of nettle extract, thalled Ascophyllum, an acerola extract, and fruit of Malpighia punicifolia, so as to obtain a nettle extract comprising from 0.05% to 50% by weight of polyphenols total, based on the total weight of said nettle extract obtained, an ascophyllum extract comprising from 1% to 70% by weight of total polyphenols, relative to the total weight of said ascophyllum extract obtained, and an acerola extract comprising from 1% to 80% by weight of vitamin C, relative to the total weight of said acerola extract obtained.