FR2950360B1 - Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules - Google Patents

Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules

Info

Publication number
FR2950360B1
FR2950360B1 FR0956522A FR0956522A FR2950360B1 FR 2950360 B1 FR2950360 B1 FR 2950360B1 FR 0956522 A FR0956522 A FR 0956522A FR 0956522 A FR0956522 A FR 0956522A FR 2950360 B1 FR2950360 B1 FR 2950360B1
Authority
FR
France
Prior art keywords
protein
biogenesis
microarn
visualization
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0956522A
Other languages
English (en)
Other versions
FR2950360A1 (fr
Inventor
Jerome Cavaille
Cavaille Marie Line Bortolin
Clement Bellemer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR0956522A priority Critical patent/FR2950360B1/fr
Priority to EP10770603A priority patent/EP2480670A1/fr
Priority to US13/497,669 priority patent/US20120202209A1/en
Priority to PCT/FR2010/051974 priority patent/WO2011036398A1/fr
Publication of FR2950360A1 publication Critical patent/FR2950360A1/fr
Application granted granted Critical
Publication of FR2950360B1 publication Critical patent/FR2950360B1/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR0956522A 2009-09-22 2009-09-22 Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules Expired - Fee Related FR2950360B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR0956522A FR2950360B1 (fr) 2009-09-22 2009-09-22 Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules
EP10770603A EP2480670A1 (fr) 2009-09-22 2010-09-21 Procédé d'imagerie cellulaire pour la visualisation de la biogenèse des microarns dans les cellules
US13/497,669 US20120202209A1 (en) 2009-09-22 2010-09-21 Cell imaging method for viewing microrna biogenesis in the cells
PCT/FR2010/051974 WO2011036398A1 (fr) 2009-09-22 2010-09-21 Procédé d'imagerie cellulaire pour la visualisation de la biogenèse des microarns dans les cellules

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0956522A FR2950360B1 (fr) 2009-09-22 2009-09-22 Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules

Publications (2)

Publication Number Publication Date
FR2950360A1 FR2950360A1 (fr) 2011-03-25
FR2950360B1 true FR2950360B1 (fr) 2011-11-25

Family

ID=42104603

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0956522A Expired - Fee Related FR2950360B1 (fr) 2009-09-22 2009-09-22 Procede d'imagerie cellulaire pour la visualisation de la biogenese des microarns dans les cellules

Country Status (4)

Country Link
US (1) US20120202209A1 (fr)
EP (1) EP2480670A1 (fr)
FR (1) FR2950360B1 (fr)
WO (1) WO2011036398A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2964114A1 (fr) * 2014-10-09 2016-04-14 Anthrogenesis Corporation Exosomes de cellules adherentes derives du placenta et utilisations associees

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6573043B1 (en) * 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US20070077582A1 (en) * 2005-09-16 2007-04-05 Primera Biosystems, Inc. Method for quantitative detection of short RNA molecules
AU2008232316A1 (en) * 2007-03-26 2008-10-02 Newcastle Innovation Limited Therapeutic targets and molecules

Also Published As

Publication number Publication date
US20120202209A1 (en) 2012-08-09
WO2011036398A1 (fr) 2011-03-31
FR2950360A1 (fr) 2011-03-25
EP2480670A1 (fr) 2012-08-01

Similar Documents

Publication Publication Date Title
ZA201606403B (en) Pluripotent stem cell culture on micro-carriers
MX2010008099A (es) Anticuerpos y angiopoyetina-2 estabilizados y sus usos.
WO2009135074A3 (fr) Micro-organismes pour la production d'acide méthacrylique
MX2018010529A (es) Composiciones y metodos para la produccion y administracion de acido ribonucleico.
MX342038B (es) Metodo para monitorizar proceso de esterilizacion.
UA106613C2 (uk) Сферичні блоки для унітаза, спосіб їх виготовлення і підвісні контейнери зі сферичними блоками для унітаза
BRPI0922572A2 (pt) método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
WO2010089151A8 (fr) Bioréacteur pour la culture de cellules de mammifère
WO2011150241A8 (fr) Réduction du niveau de lactate et augmentation de la production de polypeptides par régulation négative de l'expression de la lactate déshydrogénase et de la pyruvate déshydrogénase kinase
IL212387A0 (en) Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products
ES2527592T3 (es) Métodos basados en microARN para el diagnóstico del cáncer de colon
WO2010096588A3 (fr) Milieu de culture cellulaire contenant des combinaisons de protéines
EA201290602A1 (ru) КОМПОЗИЦИИ ДЛЯ НАПРАВЛЕННОЙ ДОСТАВКИ МАЛЫХ ИНТЕРФЕРИРУЮЩИХ РНК (миРНК)
ES2523998T3 (es) Método de aislamiento de citoblastos dérmicos
WO2010019439A3 (fr) Prédicteur du moment de récolte de cellules cultivées dans un bioréacteur
HK1171790A1 (zh) 用於 蛋白表達的細胞培養基
CL2014001564A1 (es) Metodo de cosecha de cultivo celular de un mamífero que comprende cultivar células de mamífero que expresan una proteína recombinante en un medio de cultivo celular por un tiempo predeterminado.
EP2460900A4 (fr) Acier inoxydable pour des séparateurs de pile à combustible qui présente une excellente conductivité et une excellente ductilité et son procédé de fabrication
BRPI0819648A2 (pt) sistema de co-cultura, e, métodos de cultura de levedura e produção de halogeneto de metila
EP2579495A3 (fr) Procédé et appareil pour générer une rétroaction dans un système de communication
IN2012DN00852A (fr)
FR2946362B1 (fr) Photobioreacteur,notamment pour la croissance et le developpement de microorganismes photosynthetiques
EP3272885A3 (fr) Procédés d'isolement, d'identification et de quantification des miarn
AU2010202926B2 (en) Markers and methods relating to the assessment of Alzheimer's disease
WO2011119986A3 (fr) Procédés de culture et d'analyse de cellules

Legal Events

Date Code Title Description
ST Notification of lapse

Effective date: 20150529