FR2906143A1 - Binary process useful to (re)synchronize the skin and the whole body, comprises applying a night formulation to stimulate and/or activate sirtuin and applying a day formulation to inhibitor the same sirtuins - Google Patents

Abstract

Binary process for cosmetic, pharmaceutical or dermo-cosmetic treatment to synchronize or resynchronize the skin and the whole body, comprises applying a formulation to be used at night, having stimulation and/or activation properties of sirtuins and applying a formulation to be used in day, having inhibitory properties of the same sirtuins. Independent claims are included for: (1) a composition for the implementation of the process comprising a formulation to be used in the night containing isoflavones, flavones, flavanones, cathechins and tannins, chalcone, stilbenes, oligostilbenes, stilbenoids and anthocyanidines having an effect of stimulation and/or activation of sirtuins; and (2) a composition for the implementation of the process comprising a formulation to be used in day containing nicotinamide, nicotinic acid, niacin, N-methyl nicotinamide, different nicotinates (ethyl, methyl, tocopheryl), sphingolipids i.e. sphingosine, phytoshingosine and phytosphingosine ascorbate or any other compound having inhibitory effect of sirtuins and/or derived from the given molecules. ACTIVITY : Dermatological. MECHANISM OF ACTION : Sirtuin activator; Sirtuin inhibitor.

Description

The present invention relates to a cosmetic binary composition.

  pharmaceutical and dermo-cosmetic consisting of two formulas whose joint use during the same nycthemeron has the effect of synchronizing or resynchronizing the circadian rhythms of the skin and allow it to optimize its activity and be less tired.

  It is known that the skin has many circadian rhythms concerning its activity and physiology (see: Touitou Y. Soudant et al., Circadian rhythms in a set of biochemical and biophysical skin variables (including transepidermal water loss) with: Noninvasive Methods in Healthy Young Women In: E. Haus (cd), Biological Rhythms and Medications, 6th Int.Am Amalia Island FA July 1994. Abstract book, XI-5 and also Yosipovitch G et al. In this article, we discuss the effects of transepidemal water on skin surface pH and skin temperature J Invest Dermatol 1998: 110: 20-3 and Yosipovich G Circadian rhythms of skin Cosmetics and Toiletries 1999: 114: 45-6). During the day, it plays a protective role while it is renewed at night (see: Reinberg A Circadian rhythms of the human skin: adaptive value of its temporal organization to the periodic variations of the environment In: Schmitt D Biology of human skin INSERM Paris, 1997: pp 267-28) Many pathologies are accompanied by desynchronization. from simple fatigue to cancer and Alzheimer's. There are already cosmetics using these biological rhythms for better efficacy (see: Reinberg A. Koulbanis C. Soudant E et al., Da) -night differences in the effects of cosmetic treatments on facial skin. Effects on facial skin appearance Cluronobiology International 1990: 7: 69-79) Among the most pronounced chronobiological and important rhythms for the good functioning of the epidermis, that of cellular divisions holds a special place (see: Frentz G, Moller U. HQlmich P et al. Circadian rhytluns in human epidermal cell proliferation Acta Demi Venereol1991: 71: 85-87). For the man in good health the maximum of the cellular divisions of the epidermis is reached during the night and the minimum during the day. This rhythm of cell division or mitosis is modified during pathology (see on this subject: Zagula-Mally Z. Cardoso SS, Williams D et al Time point differences in skin mitotic activity of actinium 30 keratosis and skin cancers. In: A. Reinberg, F. Halberg (eds), Chronopharmacology, Pergamon Press, Oxford, 1979, pp. 399-402), but also in the case of shift work or jet lag during trips after which epidermal cells still divide according to the previous rhythm for a longer or shorter time. Research is directed toward drugs that can resynchronize the body such as melatonin agonists (Reinberg A. et al., J. Biological Rhythms, PUF, Paris, 1st Ed, 1957. 7th Ed 1997). But the interest of melatonin is controversial and is not allowed in some countries like France. However, the resynchronization of rhythms is a reality since we are able to restore the biological rhythms and the balance of the whole organism in the seasonal depressive syndrome thanks to the strong light. Some genes and corresponding proteins also play a role in the regulation of cell and epidermal divisions, for example the genes expressing sirtuins. It is known from studies on simple organisms such as baker's yeast (S. ce revisiae), nematodes (C. elegans) or Drosophila (D. melanogaster) that their longevity can be increased by a calorie restriction and by stimulating certain Sirtuin family genes (SIR2 for silent information regulator 2) (see, for example, Howitz et al., Small molecules activators of sirtuins extend Saccharomyces cervisine lifespan .-- Nature # 23.191-196.2003) These genes would appear to have been conserved during evolution and they are present in mammals including humans where at least 8 genes have been identified. The Sirtl gene is the equivalent of the Sir2 gene of yeast. They are expressed in others in the skin by keratinocytes and fibroblasts. Sirtuins act by deacetylating various molecules which are then inactivated and particularly the p53 tumor suppressor protein as well as FOXO transcription factors (see for example for p53: Langley E .. Human SIR2 deacetylates p53 and PML antagonists / p53-induced cellular senescence.The ElIBO.Journal 21, 2383-2396, 2002 and for FOXO: Brunet A. et al .. Stress-Dependent Regulation of FOXO Transcription Factors by the SIRTI Deacetylase Science 303: 2011-20152004). P53 (or TP 53 for tumor protein 53), often referred to as the guardian of the genome, plays a central role in cellular responses to genotoxic stress. She triggers. depending on the context. apoptosis or stopping the cell cycle.

  FOXO (for Forkhead box sub-group O) is a family of transcription factors comprising in humans 4 members (FOXOI, FOXO3, FOXO4 and FOXO6). The cellular functions of FOXO which are also the stop of cell division and apoptosis. Sirtuins also work by deacetylating the histones. proteins related to DNA and regulating its functioning. When the histones are deacetylated, there is a repression of a whole series of pre-programmed genes leading to cell proliferation. On the contrary, when the histones are hyperacetylated, there is an activation of the transcription of this same series of genes and the cell proliferation ceases leaving room for either apoptosis or cell differentiation (see: Gabrielli B.et When p53 and FOXO are inactivated, cell proliferation is accelerated (see, for example, for FOXO, Hu MC, et al., IkappaB kinase). tumorigenesis through inhibition of FOXO3a forkhead, Cell 117. 225-37.2004 and see for example p53 Larsen CJ.The ARF-p53 pathway: a line of defense against oncogenic signals Pathol Biol: 48: 308-17, 2000). Activation or stimulation of sirtuins therefore results in a decrease in p53 and FOXO activity and in the deacetylation of histones and thus causes an increase in cell divisions. Conversely, an inhibition of sirtuins results in an increase in p53 and FOXO activity and in a hyperacetylation of the histones and thus causes a slowing down of the cell divisions Thus, the patent 20050136537 Composition for manipulating the lifespan and stress 2906143 3 response of cells and organism describes two opposite strategies for: - either stimulating by sirtuin activating agents (STACs for Sirtuin activating compounds) in order to reduce the activity of p53. apoptosis and increase the longevity of cells and organisms. or by siruine inhibitory agents to increase p53 activity, apoptosis and cancer at the cellular level or organisms. Sirtuin activating STACs are small molecules, the best known of which are derived from stilbene. Stilbenes are a class of molecules with a number of biological properties and are quite common in plants. The best known and most studied is resveratrol, which is 3,4,5-trihydroxystilbene. and found in a large number of plants. A number of these plants also synthesize oligomers of resveratrol. defined as oligostilbenes when they have a stilbene bond. and stilbenoids when stilbene double bonds are involved in a polymerization reaction. Plants can also synthesize resveratrol analogs such as epsilon-viniferin. R-Viniferine. R2-Viniferin, Anigopreissin A. Ampelopsin, Amurensin. Balanocarpol. Betulifol Canaliculatol. Caraganaphenol. Cassigerol. Copalliferol, Distichol, Flexuosol. Gnetin. Heimiol A. Hemsleyanol. Heyneanol A. Hopéaphénol. Hopéaphénol, Isohopeaphenol, Isohopéaphénol A, Isorhapontigenin, Kobophenol. Miyabenol, Pallidol. Parviflorol, Piceatannol, Piceid, Rhapontigenin. Stemonoporol. Suffruticosol, Vaticaffinol. Vaticanol. Vaticaphenol, alpha-Viniferin. Visitifuran. Vitisin, 5,4'-dihydroxy-3-methoxystilbene. and 3,5-dihydroxy-4'-methoxystilbene. Resveratrol is claimed for many biological activities: -Vase-relaxing. "Endothelium-dependent vasodilator effects of Resveratrol, a natural phenolic component of red wines in aorta rat" Orallo et al., Cardiovascular phar- nactia PA-56 pp.127. Anti-oxidant Antioxidant action of resveratrol on lipoperoxidation J.P. Blond and Al. Food Sciences. (1995). 347-358. -Anti-inflammatory, Molecular and cellular effects of Resveratrol in inflammation J.Schwager and Al. MIS 2002. Rennes. France. May 2002. It is also claimed in patents skin care products such as FR 2766176 which claims certain resveratrol esters or oligomers for their anti-radical activity. antioxidant, vasoprotective. FR 2777186 (L'Oreal) claims from 0.001 to 10% of hydroxystilbenes and hydroxyalkyl derivatives to stimulate collagen synthesis. stimulate the proliferation of fibroblasts. and inhibit the expression of proteases and metalloproteinases of the extracellular matrix of the dermis. L'Oréal claims the same compounds in the same proportions in patent FR 2777184 for reducing the adhesion of microorganisms on the skin and the mucous membranes. and in the patent FR 2277183. the increase of the desquamation of the skin and finally in the patent FR 2796278 the inhibition of the glycation of proteins. FR 2778337 claims the polyhydroxy stilbenes. monomers or polymers as antagonists of the aryl hydrocarbon receptor ligands.

U.S. Patent 6,085,821 claims resveratrol and piceatanol as a myeloperoxidase inhibitor. Patent FR 15098000 describes resveratrol and its oligomers as inhibitors of the enzyme Salpha reductase. Patent FR 2867977 describes resveratrol and its derivatives as an inhibitor of muscle contractions. Epsilon-Viniferin is claimed in patents (already cited) alongside resveratrol. The epsilon-viniferin is also claimed in the patent FR 2844714 as hormone-like and in the patent FR 2844 714 to prevent and / or fight against various conditions of the skin and its appendages, conditions such as reducing the thickness , suppleness, elasticity, firmness of the skin. or as microbial infections. like the excess of keratin. or to fight against pollution or glycation. The epsilon-viniferin which is a dimer (oligomer) of resveratrol, is cited in the scientific literature as having a number of biological activities: -Protection of hepatocytes against the aggressions of CC 14 (Oshima Y. et al, Experimentia: vol 51; 15 n 1; pp 63-66; 1995) -Anti-bacterial activity (Sultanbawa et al., Phytochemistry; 26: 3: 1987: 799-802) -Anti-tumoral and hepatoprotective activity (Masayaoshi Ohyama et al. , Bioorganic @ Mec / le / na / Chemistry Letters: 9: 1999: 3057-3060) -Anti-fungal (Baia AEA et al., Journal of Physiopathology; 2000, vol 148. No. 1, pp. 29-32).

The disadvantage of the first strategy and of all those claiming stimulation of sirtuins with compounds such as resveratrol, epsilon-viniferine or the like (STACs) is deacetylation. by sirtuins, histones, p53 tumor suppressor protein and FOXO transcription factor resulting in a detrimental increase in the maintenance of problem cells capable of generating tumors whereas normally they should have been eliminated (apoptosis) or stopped in their multiplication (stopping of the cell cycle). This risk is even more important when cells or organisms are subjected to significant stress exceeding or saturating the limits of repair of the cell or the body as it happens for the skin during the day. The disadvantage of the second strategy and all those claiming inhibition of sirtuins is a shortening of cellular longevity and the body. Research and experiments conducted by the author of the present invention have shown that the skin specializes temporally in the nycthémère giving priority to its protection during the day and its repair during the night (see for example Reinberg AE. Soudant E. et al Circadian dosing lime dependency in the forearm skin penetration of methyl and hexyl nicotinate Life Sciences 57, 1507-1513, 1995). Surprisingly, the inventor has found a better general state of the skin and the whole organism when conjunctive sirtuin stimulators at night and syrupine inhibitors during the day. He found that the skin and the whole organism were better synchronized, better repaired but also less tired. They recovered more quickly 40 a good state after temporary illnesses or fatigues.

He also noticed that during jet lag, the skin and the whole organism returned more quickly to normal functioning when both a sirtuin stimulator during the night of the arrival country and an inhibitor were used. sirtuine during the day. The present invention relates to a pharmaceutical and dermo-cosmetic cosmetic binary treatment process having the effect of resynchronizing the circadian rhythms of the skin and of the whole organism, enabling them to optimize their activities and to be less tired: Binary complex cosmetic, pharmaceutical and dermo-cosmetic according to the invention causes during the same nycthémère, on the one hand an increase in cellular divisions of the skin by stimulation or activation sirtuines during the night and on the other hand an inhibition of these 10 divisions by inhibition of these sirtuins during the day to the way that it happens for a healthy epidermis. The skin and the whole organism are thus better synchronized or resynchronized. The binary complex consists of 2 packaged formulas: - either in a single container comprising 2 compartments. one dedicated to the formula to be used on the day and the other containing the formula reserved for the night-either in 2 separate containers marketed in the same packaging-or still any system allowing the sale and the joint use of two formulas when of the same nycthemeron. The binary cosmetic, pharmaceutical and dermo-cosmetic composition according to the invention can be in suitable containers such as bottles, bottles, jars, boxes. capsules, capsules, ampoules, wipes. pencils, sticks. nonwoven support impregnated with a freeze-dried emulsion or not; The formula for use at night and stimulating or activating sirtuins preferably contains, but is not limited to, one or more of the molecules of the following group as well as any extract comprising one or more substances from the group consisting of isoflavones, flavones, flavanones. catechins and tannins. chalcones. stilbenes. oligostilbenes, stibenomids and anthocyanidins, for example resveratrol, epsilon viniferin. piceatannol, pinosylvin, isoliquirigenin, butine. fisetine and quercetin. Certain extracts of fenugreek, certain rice proteins or peptides, and in general all substances entering the family of STACs (Sirtuin activating compounds) as defined in the article (Howitz et al.). Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan Nature -125, 191-196.

11 September 2003) or in general any other compound derived from the above molecules (such as their acetates, glucosides or oligomers) and having an effect of stimulating and / or activating sirtuins may also be preferred.

The stimulating and activating effect of sirtuins can be measured in the manner known to those skilled in the art and as for example by a histone deacetylase activity (HDAC) assay as described in the article already cited. (Howitz et al., Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan, Nature 425, 191-196.

11 September 2003) and adapted to fibroblasts and / or keratinocytes.

The plant extracts containing the flavonoids mentioned above and activating sirtuins will also be preferable when forming formulas intended to be applied at night, for example the concentrated red wine produced as a food flavoring or plant extracts. like broccoli, tea. nuts. peanut. blueberry, vine shoot. of leaf and buds of vine, etc ....

This formula should not, however, contain any substance from the group of sirtuin-inhibiting substances and extracts such as those to be used on the day and listed below. Other cell division promoting substances may also be associated with the foregoing actives used at night such as, but not limited to, retinol and other retinoid-like molecules when they stimulate mitosis. The formula for daytime use and sirtuin-inhibiting preferably contains, but is not limited to, one or more of nicotinamide, nicotinic acid or niacin. N-methyl nicotinamide. the different nicotinates (ethyl, methyl tocopherol, etc., etc.). sphingolipids, including sphingosine, phytosphingosine and phytosphingosine ascorbate or, in general, any other compound derived from the preceding molecules and having a sirtuin-inhibiting effect. The formula for daytime use and sirtuin-inhibiting preferably contains, but is not limited to, one or more extracts having B3 vitamin activity and such as, for example, milk or aqueous fractions (aqueous extracts) of various plants conveniently free of vitamin B3. their proteins (deproteinized) and their possible flavonoids: aqueous extracts of baker's yeast - aqueous extracts of rye aqueous extracts of barley aqueous extracts of oats - aqueous extracts of wheat aqueous extracts of rice deproteinized aqueous extracts of rice bran deproteinized - aqueous extracts of wheat bran aqueous extracts of cabbage from aqueous extracts of carrots - aqueous extracts of kohlrabi aqueous extracts of spinach aqueous extracts of beans 35 aqueous extracts of pea aqueous extracts of lentils of aqueous extracts of soybean extracts aqueous of pears aqueous extracts of apples 40 aqueous extracts of apricots 2906143 7 aqueous extracts of lemons aqueous extracts of plums from aqueous extracts of peaches - aqueous extracts of figs 5 aqueous extracts of prunes from aqueous extracts of dates For cereals the corresponding flours may also be used with interest as filler or protective powder included or not in an emulsion or in a makeup product. The sirtuin inhibition effect can be measured in the manner known to those skilled in the art and as for example by a histone deacetylase (HDAC) activity assay as described in the article already cited (Howitz et al. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan, Nature 425. 191-196.

11 September 2003) and adapted to fibroblasts and / or keratinocytes placed in a situation where the expression of sirtuins is stimulated. Other cell division inhibiting substances may also be associated with the foregoing actives used in the day such as, for example, proliferation inhibitors which are found in plant extracts and which are known to humans. binary cosmetic, pharmaceutical and dermo-cosmetic according to the invention can be made in different forms. especially in the form of an oil-in-water (O / W) or water-in-oil (W / O) emulsion. multiple emulsion (W / O / H, W / O / W). microemulsion, twin-phase emulsion, PIT emulsion, nanoemulsion, pseudoemulsion (stable dispersion of two immiscible phases by means of gelling agent). of aqueous gel. of oily gel. hydroalcoholic gel, fatty phase, suspension, liquid or solid soap, foaming solution or not gel or freeze-dried emulsion and / or one of the preceding forms containing microcapsules, nano-capsules. liposomes, ethosomes. Compact or non-makeup-type powders may also be made for the formula corresponding to the day. The two formulas constituting the binary composition can be made in the same form or two different forms. The cosmetic, pharmaceutical and dermocosmetic binary composition according to the invention can be in the form of creams, liquids, pastes, lotions, emulsions or lotions. oils, gels, solids, powders. masks. stick, sprays, aerosol. The two formulas constituting the binary composition may be in the same form or in two different forms. They can have either the same color or have two different colors. Likewise they may have the same odor or be scented by different scents. The cosmetic composition. pharmaceutical and dermocosmetic according to the invention may be present, in order to improve the state of the skin more generally, in the form of a formula that can be used orally, for example capsules. tablets, syrups. According to a first embodiment of the invention the binary composition is composed of: 2906143 8 For the nocturnal formula: Acrylate C10-30 0.50 g - Resveratrol 0.001g - Ethoxydiglycol 5.00 g 5 -Extract red wine 0.20 g - Alcohol 2.00 g - Quercetin 0.01 g - Butylene glycol 0.24 g - Retinol palmitate 0.5g 10 - Carbomer 0.126 g - EDTA 0.005 g - Preservative 0.86 7 g - Macadamia oil 0.5 g - Hydrogenated lecithin 0.09 g 15 - Cyclomethicone 0.20 g - TEA 99% 0.62 g - Black tea extract 5.00 g - A dyes 0.0002 g - Demineralized water qs 100 g 20 For the diurnal formula: - Acr) late C10-30 0.50 g - Nicotinami of 0.001g - Ethoxydiglycol 5.00 g - Aqueous baker's yeast extract 0.20 g 25 - Alcohol 2.00 g - Methyl Nicotinate 0.01 g - Butylene glycol 0.24 g -Carbomer 0.126 g - EDTA 0.005 g 30 - Preservative 0.867 g - Macadamia oil 0.5 g - Hydrogenated lecithin 0.09 g - Cyclomethicone 0.20 g TEA 99% 0.62 g 35 - Soy extract 5.00 g - Coloran ts B 0.0002 g - Demineralized water qs 100 g These 2 formulas are presented in the same container comprising two compartments reserved for each of these formulas. The dyes A and B being different.

After 4 weeks of use over 72% of users perceive an improvement of at least a brand of fatigue such as wrinkles. the radiance of the complexion, the hydration of the skin and the firmness. It also occurs as if the fatigue of the whole organism was reduced, mood and sleep improved in the majority of subjects who used this topical treatment. According to a second embodiment, the binary composition is composed of: For the night-time formula: Carboxypolymethylene or carboxilic polymer 0.40 g Diethanolamine cetyl phosphate 1.50 g Octyl dodecanol 5.50 g Alcohol cetane 1.00 g Mineral oil 2.00 g Stearic acid 1.00 g Ethoxylated stearic alcohol (20 E) 0.30 g - Keto-stearic alcohol} Tick 1.80 g Preservative 0.15 g 15 Macadamia oil 0.50 g Tri ethanolamine 0.60 g Cyclomethicone 12.00 g Dimethicone copolyol 3.00 g - Phenyl trimetone 2.00 g Black tea extract 5.00 g Sepigel 305 1.00 g Rice peptide 1.00 g Butylene glycol _6.00 g Mica and titanium oxide 1.00 g 25 Fisetine 0 0001 g Demineralised water gsp 100 g For the diurnal formula: Carboxypolymeth3 Tene or polymer carboxilic acid 0.40 g Diethanolamine cetyl phosphate 1.50 g 30 - Octyl dodecanol 5.50 g - Cetyl alcohol 1.00 g Mineral oil 2.00 g Stearic acid _1.00 g - Ethoxylated stearic alcohol (20 E) 0.30 g 35 Ketostearyl alcohol 1.80 g Preservative 0.15 g Macadamia oil 0.50 g Triethanolamine 0.60 g Cyclomethicone 12.00 g 40 Dimethicone copalyol 3.00 g 2906143 10 Phenyl trimetone 2.00 g - Oat aqueous extract 5.00 g - Sepigel 305 1.00 g - Aqueous extract of apple without pol) phenol 1.00 g 5 Butylene glycol 6.00 g Mica and titanium oxide 1.00 g Nicotinic acid 0.0001 g Demineralized water gsp 100 g These 2 formulas are presented in two different containers, each containing one of these 10 two formulas but sold in the same package. After 4 weeks of use over 70% of users perceive an improvement of at least one brand of fatigue and aging such as wrinkles, radiance of complexion, skin hydration and firmness. The general state of the body is improved after 6 weeks of use and the mood in the first week of treatment in the majority of users. The day formula of the first embodiment can be associated with the night formula of second embodiment. In the same way, the day formula of the second embodiment can be advantageously associated with the night formula of the first embodiment. Four other exemplary embodiments are also set forth below: 1.) O / W emulsion: Formula to be used at night. Liquid paraffin 6 g liquid lanolin 3 g Arlacel 165 (marketed by Atlas) 6 g 25 Tween 60 (marketed by Atlas) 2 g cetyl alcohol 1.2 g stearic acid 2.5 g retinyl palmitate 0.5 g silicone oil volatile 10 g 30 - Triethanolamine 0. 1 g - Preservative 0.3 g - Antioxidants 0.3 g - Quercetin 0.003 g Fenugreek extract 5 g 35 - Broccoli extract 1 g - Demineralised water qs 100 g Formulation to be used during the day. - Liquid paraffin 6 g - Liquid lanolin 3 g 40 - Arlacel 165 (marketed by Atlas) 6 g 2906143 11 Tween 60 (marketed by Atlas) 2 g - Cetyl alcohol 1.2 g - Stearic acid 2.5 g - Phytoshingosine 0.05 g 5 - Oil volatile silicone 10 g-Triethmolamine 0.1 g - Preservative 0.3 g - Phenylbenzimidazole sulfonic acid 2g - Octal methoxycinnamate g 10 - Antioxidants 0.3 g - Nicotinamide 0.01 g - Aqueous extract of the deproteinized lens 5 g - Demineralised water qs 100 g 2.) Liposome cream : 15 For the night. Cetyl alcohol 4 g - B-sitosterol 4 g - Diketyl phosphate 0.5 g - preservative 0.3 g 20 - Carbopol 981 (marketed by Goodrich) 0.2 g - Triethanolamine 0.2 g - Phospholipid 0.05 g - Resveratrol 0.001 g - Nut extract 0.5 g 25 - Vine shoot extract lg - Demineralised water qs 100 g For the day - Cetyl alcohol 4 g - Bsitosterol 4 g 30 - Diketyl phosphate 0.5 g - Preservative 0.3 g Carbopol 981 (marketed by Goodrich) 0.2 g - Triethanolamine 0.2 g Sphingosine 0.05 g 35 - Nicotinate of tocopherol O.lg - Non-dormant narcissus extract 1g - Demineralised water qs 100 g 3.) W / O emulsion: For the night. 40 - Protégin (marketed by Goldschmidt) 19 g 2906143 12 - Glycerin 3 g - Petrolatum oil 8 g - Mg sulphate 0.5 g Fisétine 0.5 g 5 - Peanut extract 1 g - Retinyl palmitate 0.02 g Preservative 0.2 g - Demineralised water qs 100 g For the day.

Protégin (marketed by Goldschmidt) 19 g - Glycerin 3 g - Petrolatum oil 8 g - Mg sulfate 0.5 g - Nicotinamide 0.5 g 15 - Aqueous Prune extract 3 g - Aqueous apricot extract 1 g - Meth Nicotinate) the 0.01 g - preservative 0.2 g - demineralized water qs 100 g 4.) Emulsion gel O / W For the night. - Carbopol 981 (marketed by Goodrich) 0.6 g - Ethyl alcohol 15 g - Volatile silicone oil 3 g 25 - Purcellin oil 7 g - Preservative 0.3 g - Concentrated wine powder 1 g Lactic acid 0.05g -Fragrance O. 4g 30 - Resveratrol 0.00 2 g epsilon-viniferine 0.018 -Tethanolamine 0.25 g - Demineralized water qs 100 g For the day. -Carbopol 981 (marketed by Goodrich) 0.6 g Ethyl alcohol 15 g -Vegetable silicone oil 3 g - Purcellin oil 7 g - Preservative 0 3 g 40 - Perfume 0.4 g 2906143 13 - Nicotinic acid 0.01 g -Tethanolamine 0.2 g - Aqueous extract of fig 1 g - Demineralized water qs 100 g Each overnight formulation of each of these 4 examples can be advantageously associated with a day formula of one of the other three examples to form new binary compositions. 5.) Makeup powder. Without limitation, an example of non-compacted powder and having the following weight composition is given: 10 - 20 to 94% of mica, - 5 to 45% of polymer powder. - 1.5 to 5% of oil. - 0 to 4% of metallic soaps, - 0.5 to 1% of wheat flour 15 - 0.5 to 1% of rice bran flour - 02 to 1% of highly polymerised DNA - 0 to 30% of pearlescent agents. - 5% to other usual ingredients. and sufficient quantities of perfume and pigments, the sum of the percentages of the ingredients mentioned being equal to 100. This makeup powder may be associated with each of the night formulas to form a new binary composition or be associated with each binary composition. to provide a reinforcement of effectiveness to use the day when the skin is the most assaulted.

Claims (9)

  1.) Cosmetic, pharmaceutical and dermocosmetic binary treatment process intended to synchronize or resynchronize the skin and the whole organism, characterized in that it consists in applying, on the one hand, a formula for use at night and endowed with properties of stimulating and / or activating sirtuins, and secondly to apply a formula during the day and endowed with inhibiting properties of these same sirtuins.   2.) Cosmetic composition. pharmaceutical and dermal-cosmetic for the implementation of the method according to claim 1 characterized in that the formula to be applied at night contains one or more substances of the group consisting of isoflavones, flavones. flavanones, cathechins and tannins, chalcones, stilbenes, oligostilbenes, stilbenoids and anthocyanidins having an effect of stimulating and / or activating sirtuins.   3.) Cosmetic, pharmaceutical and dermo-cosmetic composition for carrying out the process according to claim 1 or claim 2 characterized in that the formula to be applied at night contains one or more substances from the group consisting of resveratrol, epsilon-viniferine, piceatannol, pinosylvine, isoquirigenin, buteine, quercetin's fisetin and / or any plant extract containing it and any other compound derived from these substances and having a stimulating effect and / or activation of sirtuins such as their acetates, their glucosides and / or their oligomers   4.) Cosmetic, pharmaceutical and dermo-cosmetic composition for carrying out the method according to claim 1 characterized in that the formula to be applied at night contains one or more plant extract from the group of extracts of vine shoots, leaves or vine buds, extracts of broccoli, fenugreek, tea, nuts, peanuts, blueberries, rice or red wine concentrate or peptide or polypeptide and any extract having a stimulating effect and / or activating sirtuins containing one or more substances according to claim 2 or 3.   5.) Cosmetic, pharmaceutical and dermal-cosmetic composition for carrying out the method according to claim 1, characterized in that the formula to be applied during the day contains one or more substances from the group consisting of nicotinamide. nicotinic acid, niacin, N-methyl nicotinamide, various nicotinates (ethyl, methyl, tocopheryl), sphingolipids including sphingosine. phytoshingosine and phytosphingosine ascorbate or generally any other compound and having a sirtuin-inhibiting effect and / or derived from the above molecules.   6.) Cosmetic, pharmaceutical and dermo-cosmetic composition for carrying out the method according to claim 1, characterized in that the formula to be applied during the day contains one or more aqueous fractions freed from their possible flavonoids and rich in vitamin 133 of the group. consisting of milk and aqueous extracts of baker's yeast, aqueous extracts of rye, barley, oats, wheat, deproteinized rice, wheat bran, deproteinized rice bran, cabbages, of kohlrabi carrots, spinach, beans, peas, lentils, soya, pears, apples, apricots, lemons, plums, peaches, figs, date prunes and any extract containing vitamin B3 and capable of inhibiting the activity of sirtuins or generally any extract containing vitamin B3 and capable of inhibiting the activity of sirtuins.   7. A cosmetic, pharmaceutical and dermo-cosmetic binary treatment process according to claim 1, characterized in that a day-day formula according to claims 5 to 6 and a different formula at night according to claims 2 to 4 are used.   8. Cosmetic, pharmaceutical and dermo-cosmetic binary treatment process according to claim 1 or 7, characterized in that each of its two formulas is either in the same form or in two different forms, in particular in the form of an emulsion. oil in water (O / W) or water in oil (W / O), multiple emulsion (W / O / W, W / O / W), microemulsion, twin phase emulsion, PTT emulsion , nanoemulsion, pseudoemulsion (stable dispersion of two immiscible phases by means of gelling agent), aqueous gel, fatty gel, hydroalcoholic gel, fatty phase, suspension, liquid or solid soap, foaming solution or not, freeze-dried gel or emulsion and / or one of the preceding forms containing microcapsules, nano-capsules, liposomes, ethosomes and which may be present without this being limiting in creams, liquids, pasta, lotions, emulsions, oils, gels, syrups, solids, powders, masks, stick, tablets, sprays, aerosol.   9. A method for cosmetic, pharmaceutical and dermo-cosmetic binary treatment according to claim 1, 7 or 8 for improving skin fatigue and optimizing the functioning of the skin and the whole organism and characterized in that The binary composition is applied during the same nycthemeron.