FR2877092B1 - METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION - Google Patents

METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION

Info

Publication number
FR2877092B1
FR2877092B1 FR0452443A FR0452443A FR2877092B1 FR 2877092 B1 FR2877092 B1 FR 2877092B1 FR 0452443 A FR0452443 A FR 0452443A FR 0452443 A FR0452443 A FR 0452443A FR 2877092 B1 FR2877092 B1 FR 2877092B1
Authority
FR
France
Prior art keywords
dye
dyes
solution
relative measurement
fluorescence yield
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0452443A
Other languages
French (fr)
Other versions
FR2877092A1 (en
Inventor
Joel Bleuse
Sophie Carayon
Eric Delamadeleine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0452443A priority Critical patent/FR2877092B1/en
Application filed by Commissariat a lEnergie Atomique CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to US11/577,926 priority patent/US20090127450A1/en
Priority to JP2007538481A priority patent/JP2008518222A/en
Priority to DE602005016136T priority patent/DE602005016136D1/en
Priority to EP05857328A priority patent/EP1805504B1/en
Priority to PCT/FR2005/050896 priority patent/WO2006092475A1/en
Priority to CNA2005800439164A priority patent/CN101084430A/en
Priority to AT05857328T priority patent/ATE440274T1/en
Publication of FR2877092A1 publication Critical patent/FR2877092A1/en
Application granted granted Critical
Publication of FR2877092B1 publication Critical patent/FR2877092B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)

Abstract

The method involves exciting a dye, having two different concentrations in a solvent, using an electromagnetic radiation. The photoluminescence of the excited dye and fluorescence signal transmission through a cell (20) containing the dye are measured. The measured data is compared with photoluminescence and transmission data of a reference dye e.g. rhodamine 6G. The relative fluorescence quantum efficiency of the dye is calculated. An independent claim is also included for a device for measuring relative fluorescence quantum efficiency of a dye.
FR0452443A 2004-10-26 2004-10-26 METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION Expired - Fee Related FR2877092B1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR0452443A FR2877092B1 (en) 2004-10-26 2004-10-26 METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION
JP2007538481A JP2008518222A (en) 2004-10-26 2005-10-25 Relative method for measuring the fluorescence quantum yield of dyes in solution.
DE602005016136T DE602005016136D1 (en) 2004-10-26 2005-10-25 PROCESS FOR THE RELATIVE MEASUREMENT OF THE FLUORESCENCE QUA
EP05857328A EP1805504B1 (en) 2004-10-26 2005-10-25 Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution
US11/577,926 US20090127450A1 (en) 2004-10-26 2005-10-25 Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution
PCT/FR2005/050896 WO2006092475A1 (en) 2004-10-26 2005-10-25 Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution
CNA2005800439164A CN101084430A (en) 2004-10-26 2005-10-25 Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution
AT05857328T ATE440274T1 (en) 2004-10-26 2005-10-25 METHOD FOR THE RELATIVE MEASUREMENT OF THE FLUORESCENCE QUANTUM EFFICIENCY OF DYES IN SOLUTIONS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0452443A FR2877092B1 (en) 2004-10-26 2004-10-26 METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION

Publications (2)

Publication Number Publication Date
FR2877092A1 FR2877092A1 (en) 2006-04-28
FR2877092B1 true FR2877092B1 (en) 2006-12-29

Family

ID=34950258

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0452443A Expired - Fee Related FR2877092B1 (en) 2004-10-26 2004-10-26 METHOD OF RELATIVE MEASUREMENT OF THE QUANTUM FLUORESCENCE YIELD OF DYES IN SOLUTION

Country Status (8)

Country Link
US (1) US20090127450A1 (en)
EP (1) EP1805504B1 (en)
JP (1) JP2008518222A (en)
CN (1) CN101084430A (en)
AT (1) ATE440274T1 (en)
DE (1) DE602005016136D1 (en)
FR (1) FR2877092B1 (en)
WO (1) WO2006092475A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5161755B2 (en) * 2008-12-25 2013-03-13 浜松ホトニクス株式会社 Spectrometer, spectroscopic method, and spectroscopic program
CN102519883A (en) * 2011-12-30 2012-06-27 南开大学 Laser dye oxazine-1 perchlorate quantum efficiency measurement method
JP6248460B2 (en) * 2013-08-08 2017-12-20 株式会社島津製作所 Excitation light irradiation device
JP2018179556A (en) * 2017-04-04 2018-11-15 三浦工業株式会社 Water quality monitoring device and water treatment system
CN108535225B (en) * 2018-03-19 2020-11-03 苏州星烁纳米科技有限公司 Method for testing quantum yield
CN108801949B (en) * 2018-03-19 2020-11-03 苏州星烁纳米科技有限公司 Method for testing quantum yield
CN108562562B (en) * 2018-03-19 2021-03-02 苏州星烁纳米科技有限公司 Method for testing quantum yield

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5485527A (en) * 1985-11-04 1996-01-16 Becton Dickinson And Company Apparatus and method for analyses of biological specimens
US4804849A (en) * 1987-01-16 1989-02-14 Biospherical Instruments Inc. Method and apparatus for determining concentrations of chlorophyll and the rate of primary production in water
US5723338A (en) * 1994-11-04 1998-03-03 Amoco Corporation Tagging hydrocarbons for subsequent identification
WO1997002482A1 (en) * 1995-06-30 1997-01-23 Biometric Imaging, Inc. Volumetric cell quantification method and system
GB9621122D0 (en) * 1996-10-10 1996-11-27 Cambridge Imaging Ltd Improvements in and relating to methods and apparatus for assay analysis
US6251303B1 (en) * 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
EP1436596A2 (en) * 2001-09-28 2004-07-14 Ciencia, Incorporated Compact multiwavelength phase fluorometer

Also Published As

Publication number Publication date
CN101084430A (en) 2007-12-05
EP1805504A1 (en) 2007-07-11
FR2877092A1 (en) 2006-04-28
DE602005016136D1 (en) 2009-10-01
JP2008518222A (en) 2008-05-29
WO2006092475A1 (en) 2006-09-08
EP1805504B1 (en) 2009-08-19
ATE440274T1 (en) 2009-09-15
US20090127450A1 (en) 2009-05-21

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Effective date: 20140630