FR2844523B1 - METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER - Google Patents

METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER

Info

Publication number
FR2844523B1
FR2844523B1 FR0306905A FR0306905A FR2844523B1 FR 2844523 B1 FR2844523 B1 FR 2844523B1 FR 0306905 A FR0306905 A FR 0306905A FR 0306905 A FR0306905 A FR 0306905A FR 2844523 B1 FR2844523 B1 FR 2844523B1
Authority
FR
France
Prior art keywords
detection
water
complex mixture
identification
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR0306905A
Other languages
French (fr)
Other versions
FR2844523A1 (en
Inventor
Stephane Jean Claude Bonilla
Sebastien Alain Christian Bouton
Debitte Nadine Batisse
Gabriel Festoc
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pall Genedisc Technologies SAS
Original Assignee
GeneSystems SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0211400A external-priority patent/FR2844522A1/en
Application filed by GeneSystems SA filed Critical GeneSystems SA
Priority to FR0306905A priority Critical patent/FR2844523B1/en
Priority to AU2003282157A priority patent/AU2003282157A1/en
Priority to PCT/FR2003/002709 priority patent/WO2004024944A2/en
Publication of FR2844523A1 publication Critical patent/FR2844523A1/en
Application granted granted Critical
Publication of FR2844523B1 publication Critical patent/FR2844523B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé pour la détection, l'identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l'eau, ledit procédé comprenant une étape d'amplifications multiples, simultanées mais séparées dans l'espace, de séquences d'acides nucléiques cibles spécifiques et exclusives. La présente invention a en outre pour objets des couples d'amorces pour amplification spécifiques et exclusifs, utiles à la mise en oeuvre dudit procédé, ainsi que des sondes nucléotidiques pour l'amplification en temps réel ou la détection de séquences d'acides nucléiques spécifiques et exclusives des bactéries contenues dans un mélange complexe ou dans de l'eau. L'invention concerne enfin des outils de biologie moléculaire, à savoir un kit d'analyse et des micro-réseaux ou puces à acides nucléiques pour la détection, l'identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l'eau.The invention relates to a method for the detection, identification and, where appropriate, quantification of bacteria contained in a complex mixture or in water, said method comprising a step of multiple amplifications, simultaneous but separate in the space, specific and exclusive target nucleic acid sequences. The present invention also relates to specific and exclusive pairs of primers for amplification, useful for the implementation of said method, as well as nucleotide probes for the amplification in real time or the detection of specific nucleic acid sequences. and exclusive of bacteria contained in a complex mixture or in water. The invention finally relates to molecular biology tools, namely an analysis kit and microarrays or nucleic acid chips for the detection, identification and, where appropriate, quantification of bacteria contained in a complex mixture. or in water.

FR0306905A 2002-09-13 2003-06-06 METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER Expired - Lifetime FR2844523B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR0306905A FR2844523B1 (en) 2002-09-13 2003-06-06 METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER
AU2003282157A AU2003282157A1 (en) 2002-09-13 2003-09-12 Method and nucleotide sequences for the detection and identification of micro-organisms in a complex mixture or water
PCT/FR2003/002709 WO2004024944A2 (en) 2002-09-13 2003-09-12 Method and nucleotide sequences for the detection and identification of micro-organisms in a complex mixture or water

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0211400A FR2844522A1 (en) 2002-09-13 2002-09-13 Multiplex detection and identification of bacteria in complex mixtures, useful for testing food and environmental samples, by simultaneous, but spatially resolved amplification
FR0306905A FR2844523B1 (en) 2002-09-13 2003-06-06 METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER

Publications (2)

Publication Number Publication Date
FR2844523A1 FR2844523A1 (en) 2004-03-19
FR2844523B1 true FR2844523B1 (en) 2011-01-14

Family

ID=31947976

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0306905A Expired - Lifetime FR2844523B1 (en) 2002-09-13 2003-06-06 METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER

Country Status (3)

Country Link
AU (1) AU2003282157A1 (en)
FR (1) FR2844523B1 (en)
WO (1) WO2004024944A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0710889A2 (en) * 2006-04-24 2011-08-16 Sigma Alimentos Sa De Cv method for multiple and simultaneous detection and quantification of pathogens by real time polymerase chain reaction
ES2366798T3 (en) * 2006-05-29 2011-10-25 Bio-Rad Pasteur MULTIPLE DETECTION IN REAL TIME OF THREE BACTERIAL SPECIES RESPONSIBLE FOR SEXUAL TRANSMISSION DISEASES.
EP2358908B1 (en) 2008-11-14 2014-01-08 Gen-Probe Incorporated Compositions and methods for detection of campylobacter nucleic acid
FR2970264B1 (en) 2011-01-12 2014-09-05 Ct Scient Tech Batiment Cstb METHOD AND FRAGMENTS OF NUCLEOTIDES FOR DETECTION AND / OR QUANTIFICATION OF LEGIONELLA HOSTS, KIT THEREFOR

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2812306B1 (en) * 2000-07-28 2005-01-14 Gabriel Festoc POLYMERSIS CHAIN AMPLIFICATION SYSTEM OF TARGET NUCLEIC SEQUENCES

Also Published As

Publication number Publication date
WO2004024944A2 (en) 2004-03-25
AU2003282157A1 (en) 2004-04-30
FR2844523A1 (en) 2004-03-19
WO2004024944A3 (en) 2004-09-16
AU2003282157A8 (en) 2004-04-30

Similar Documents

Publication Publication Date Title
EP2474630A3 (en) Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof
EP1362929A3 (en) Methods for genotyping
MXPA04008477A (en) Methods for detection of genetic disorders.
WO2004078994A3 (en) Methods for detection of genetic disorders
ATE358733T1 (en) RAPID METHOD FOR DETECTING MICROORGANISMS IN FOOD SAMPLES
TW200942621A (en) Apparatus for nucleic acid sequencing by Raman spectroscopy
EP1046717A3 (en) Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method
CA2497988A1 (en) Quantification of gene expression
WO2002086163A8 (en) Methods for high throughput genome analysis using restriction site tagged microarrays
GB9903578D0 (en) Detection system
EP1670947A4 (en) Methods and compositions for the correlation of single nucleotide polymorphisms in the vitamin k epoxide reductase gene and warfarin dosage
WO2003048300A3 (en) Methods of identifying cellular target molecules
AU2002303444A1 (en) Detection of microsatellite instability and its use in diagnosis of tumors
WO2003027638A3 (en) Methods for detecting genetic mosaicisms using arrays
CA2303414A1 (en) Non-competitive co-amplification methods
AU6975694A (en) Salmonella identification by the polymerase chain reaction
IL145607A0 (en) Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
AU2003289927A1 (en) Multiplex assay detection of pathogenic organisms
FR2844523B1 (en) METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER
HK1068377A1 (en) Analytical method and kit
GB0223563D0 (en) Detection system
EP1531180A4 (en) Method of diagnosing risk of myocardial infarction
WO2003074739A3 (en) Automated allele determination using fluorometric genotyping
WO2002079512A3 (en) Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr
AU2002231689A1 (en) In situ hybridization system for specifically detecting microorganisms

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 14

PLFP Fee payment

Year of fee payment: 15