FR2827873B1 - AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER - Google Patents

AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER

Info

Publication number
FR2827873B1
FR2827873B1 FR0109841A FR0109841A FR2827873B1 FR 2827873 B1 FR2827873 B1 FR 2827873B1 FR 0109841 A FR0109841 A FR 0109841A FR 0109841 A FR0109841 A FR 0109841A FR 2827873 B1 FR2827873 B1 FR 2827873B1
Authority
FR
France
Prior art keywords
pathogenic organisms
unit
rna
detecting
concentrating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0109841A
Other languages
French (fr)
Other versions
FR2827873A1 (en
Inventor
Marie Christine Huau
Thomas Schalkhammer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe dAmenagement Urbain et Rural SAUR SAS
Original Assignee
Societe dAmenagement Urbain et Rural SAUR SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0109841A priority Critical patent/FR2827873B1/en
Application filed by Societe dAmenagement Urbain et Rural SAUR SAS filed Critical Societe dAmenagement Urbain et Rural SAUR SAS
Priority to PCT/IB2002/003424 priority patent/WO2003010278A2/en
Priority to AT02758725T priority patent/ATE486950T1/en
Priority to US10/484,869 priority patent/US20040241828A1/en
Priority to AU2002324289A priority patent/AU2002324289A1/en
Priority to DE60238199T priority patent/DE60238199D1/en
Priority to EP02758725A priority patent/EP1412470B1/en
Publication of FR2827873A1 publication Critical patent/FR2827873A1/en
Application granted granted Critical
Publication of FR2827873B1 publication Critical patent/FR2827873B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom

Abstract

Detecting the presence of pathogenic organisms in water sample, involves concentrating pathogenic organisms, breaking to open cells of pathogenic organisms, concentrating DNA and/or RNA, amplifying nucleic acid and detecting DNA and/or RNA by qualitatively and/or quantitatively. The process is controlled fully automatically by using a control logic system. An Independent claim is included for a device for detecting the presence of pathogenic organisms in a water sample. The device comprises a unit (1) for concentrating the pathogenic organisms, a unit (2) for breaking open cells from the pathogenic organisms, a unit (3) for concentrating DNA and/or RNA, a unit (4) for amplifying nucleic acids, a unit (5) for qualitatively and/or quantitatively detecting DNA and/or RNA and a control logic system (6) which controls fully automatically the sequential treatment steps carried out by units (1) to (5), pumping units (7), automatic sampling and distribution units (8), and a central control system (9).
FR0109841A 2001-07-24 2001-07-24 AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER Expired - Fee Related FR2827873B1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FR0109841A FR2827873B1 (en) 2001-07-24 2001-07-24 AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER
AT02758725T ATE486950T1 (en) 2001-07-24 2002-07-24 AUTOMATIC METHOD FOR DETECTING PATHOGENIC ORGANISMS IN WATER
US10/484,869 US20040241828A1 (en) 2001-07-24 2002-07-24 Automated process for detecting pathogenic organisms in water
AU2002324289A AU2002324289A1 (en) 2001-07-24 2002-07-24 Automated process for detecting pathogenic organisms in water
PCT/IB2002/003424 WO2003010278A2 (en) 2001-07-24 2002-07-24 Automated process for detecting pathogenic organisms in water
DE60238199T DE60238199D1 (en) 2001-07-24 2002-07-24 AUTOMATIC PROCEDURE FOR DETECTION OF PATHOGENIC ORGANISMS IN WATER
EP02758725A EP1412470B1 (en) 2001-07-24 2002-07-24 Automated process for detecting pathogenic organisms in water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0109841A FR2827873B1 (en) 2001-07-24 2001-07-24 AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER

Publications (2)

Publication Number Publication Date
FR2827873A1 FR2827873A1 (en) 2003-01-31
FR2827873B1 true FR2827873B1 (en) 2003-09-26

Family

ID=8865828

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0109841A Expired - Fee Related FR2827873B1 (en) 2001-07-24 2001-07-24 AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER

Country Status (3)

Country Link
AT (1) ATE486950T1 (en)
DE (1) DE60238199D1 (en)
FR (1) FR2827873B1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2868082B1 (en) * 2004-03-26 2006-06-09 Amenagement Urbain & Rural PROCESS FOR EXTRACTING INTRACELLULAR COMPOUNDS OR COMPLEXES FROM A SAMPLE CONTAINING CELLS IN SUSPENSION IN A LIQUID
CN106929406B (en) * 2017-05-23 2024-02-06 至农科技发展(浙江)有限公司 Compact device for nucleic acid extraction and detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2033718A1 (en) * 1990-01-19 1991-07-20 Ronald M. Atlas Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
ATE291637T1 (en) * 1997-03-24 2005-04-15 Robert E Fields BIOMOLECULAR PROCESSOR
WO1999018421A1 (en) * 1997-10-03 1999-04-15 Monterey Bay Aquarium Research Institute Aquatic autosampler device
EP1192006B1 (en) * 1999-06-22 2008-05-14 Tecan Trading AG Devices for the performance of miniaturised in vitro amplification assays
US6878517B1 (en) * 1999-12-15 2005-04-12 Congra Grocery Products Company Multispecies food testing and characterization organoleptic properties

Also Published As

Publication number Publication date
FR2827873A1 (en) 2003-01-31
DE60238199D1 (en) 2010-12-16
ATE486950T1 (en) 2010-11-15

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TP Transmission of property
ST Notification of lapse

Effective date: 20140331