FR2820432A1 - Airborne micro-organism sampler grille has safety projections to prevent it being fitted before Petri dish cover is removed - Google Patents

Airborne micro-organism sampler grille has safety projections to prevent it being fitted before Petri dish cover is removed Download PDF

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FR2820432A1
FR2820432A1 FR0101574A FR0101574A FR2820432A1 FR 2820432 A1 FR2820432 A1 FR 2820432A1 FR 0101574 A FR0101574 A FR 0101574A FR 0101574 A FR0101574 A FR 0101574A FR 2820432 A1 FR2820432 A1 FR 2820432A1
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petri dish
grid
grille
impactor
holes
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FR2820432B1 (en
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Thierry Roger Garcia
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L5/00—Gas handling apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Clinical Laboratory Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The airborne micro-organism sampler has an impacter grille (5) with holes (7), designed to be fitted over a Petri dish (1) with a culture medium. Prior to use the Petri dish is protected by a safety cover (3), and the grille, made from a material able to withstand autoclaving temperatures e.g. of 134[deg] C for 5 min, has projections (6) on its underside to prevent it being fitted to the Petri dish holder before the cover has been removed.

Description

<Desc/Clms Page number 1> <Desc / Clms Page number 1>

DESCRIPTION Le contrôle de l'hygiène est un facteur critique, notamment dans les milieux industriels (Agro-alimentaire, Electronique et Pharmaceutique) et Hospitaliers. Parallèlement à une évolution des conditions de travail imposant des zones d'activités de plus en plus propres du point de vue des particules en suspensions et des germes dans l'air, les appareils pour les contrôles microbiologiques de l'air ont évolués. Les résultats de ces appareils ont désormais une grande importance, car la détection d'un germe risque de mettre en péril un lot de fabrication. Les enjeux économiques et les critères de qualité étant de plus en plus sévère, il devient nécessaire d'éliminer tous risques de résultats décrit comme faux positif ou faux négatif . DESCRIPTION Hygiene control is a critical factor, particularly in industrial settings (agri-food, electronics and pharmaceuticals) and hospitals. In parallel with a change in working conditions requiring increasingly clean activity areas from the point of view of suspended particles and germs in the air, devices for microbiological air monitoring have evolved. The results of these devices are now of great importance because the detection of a germ may jeopardize a production batch. As the economic stakes and the quality criteria are more and more severe, it becomes necessary to eliminate all risks of results described as false positive or false negative.

Les consommables les plus utilisés pour les prélèvements d'air sont les boites de pétri standards diamètre 90 ou 55 et les boites de pétri de type Rodac (1) (avec une jupe et un bord extérieur (4)). Les boites standards ont un couvercle qui repose sur les cotés du fond de la boite et à l'extérieur en position fermée, les boites Rodac (1) ont un couvercle qui repose sur le fond de la boite entre le bord intérieur (2) retenant la gélose et le bord extérieure (4) de la jupe. L'utilisation des boites de pétri dans les contrôles microbiologiques de l'air est d'usage, sauf pour quelques irréductibles qui fabriquent encore un consommable spécifique à leurs appareils. The most used consumables for air sampling are standard petri dishes, diameter 90 or 55, and Rodac (1) petri dishes (with a skirt and an outer edge (4)). The standard boxes have a lid that rests on the sides of the bottom of the box and outside in the closed position, the boxes Rodac (1) have a lid that rests on the bottom of the box between the inner edge (2) retaining the agar and the outer edge (4) of the skirt. The use of petri dishes in the microbiological controls of the air is customary, except for some irreducible who still make a consumable specific to their devices.

Un inconvénient majeur dans le suivi de l'aérobiocontamination, est le faux négatif qui résulte d'un prélèvement le plus souvent fait en oubliant d'ôter le couvercle (3) de la boite de pétri, soit en oubliant de déclencher l'appareil, soit en oubliant de mettre la boite

Figure img00010001

de pétri. Les conséquences d'un tel acte sur l'interprétation finale est majeure, car elle i peut aboutir à une conclusion du type Absence de micro-organisme viable ou état stérile , ce qui conclut à un faux sentiment de sécurité qui pourra avoir des conséquences économiques importantes, comme le retrait de marchandise après distribution. A major disadvantage in the monitoring of aerobiocontamination, is the false negative that results from a sampling most often done forgetting to remove the lid (3) of the petri dish, or forgetting to trigger the device, either forgetting to put the box
Figure img00010001

of petri. The consequences of such an act on the final interpretation is major, because it can lead to a conclusion of the type Absence of viable microorganism or sterile state, which leads to a false sense of security which may have economic consequences. important, such as the withdrawal of goods after distribution.

Un autre inconvénient majeur est le faux positif, qui résulte le plus souvent d'un prélèvement fait avec une grille non stérile, le passage de l'air décroche des particules présente sur la paroi des trous d'impactions (7) et si les particules sont des germes, ils vont être détectés et comptés. Another major disadvantage is the false positive, which most often results from a sampling made with a non-sterile grid, the passage of air picks up particles present on the wall of the impaction holes (7) and if the particles are germs, they will be detected and counted.

L'interprétation du contrôle va aboutir à une conclusion d'un état de non stérile et à une destruction du lot de production. The interpretation of the control will lead to a conclusion of a non-sterile state and a destruction of the production lot.

En règle générale sur tous les appareils le couvercle de l'appareil comprend la grille, (5)

Figure img00010002

l'utilisation des termes couvercle , crible , tamis ou grille est similaire, il sera utilisé le terme grille (5) pour désigner le couvercle de l'appareil, afin de ne pas le confondre avec le couvercle (3) de la boite de pétri. As a general rule on all devices the cover of the apparatus includes the grid, (5)
Figure img00010002

the use of terms cover, sieve, sieve or grid is similar, it will be used the term grid (5) to designate the lid of the apparatus, so as not to confuse it with the lid (3) of the petri dish .

Nous avons conçu un dispositif sur la grille d'impaction rendant impossible la fermeture de la grille sur la boite de pétri si le couvercle est encore présent sur celle-ci, et nous avons opté pour une matière transparente afin de visualiser la présence ou non de la boite de pétri pendant le prélèvement. We designed a device on the impaction grid making it impossible to close the grid on the petri dish if the lid is still present on it, and we opted for a transparent material to visualize the presence or absence of the box of kneaded during the sampling.

De plus nous avons opté pour une matière pouvant être autoclavable selon les normes actuelles, c'est à dire 5 minutes à 134OC. In addition we opted for a material that can be autoclavable according to current standards, ie 5 minutes at 134OC.

Nous avons conçu un autre dispositif : La grille utilise pour les trous (7) une technique dite Tube/Plaque en opposition aux appareils dits Fente/Plaque. We have designed another device: The grid uses for the holes (7) a so-called Tube / Plate technique in opposition to devices called Slot / Plate.

<Desc/Clms Page number 2> <Desc / Clms Page number 2>

La grille (5) d'impaction de l'appareil comprend un dispositif de sécurité rendant impossible la fermeture de la grille sur la boite de pétri si le couvercle (3) est encore présent sur celle-ci. Voir figure 1 en position ouverte et figure 2 en position fermée. The grid (5) of impaction of the apparatus comprises a safety device making it impossible to close the gate on the petri dish if the cover (3) is still present thereon. See Figure 1 in the open position and Figure 2 in the closed position.

Il est réalisé de la façon suivante, ce dispositif de sécurité est composé de piétements qui lors de la fermeture de la grille (5) de l'appareil impacteur, les piétements (6) doivent se trouver en lieu et place d'une partie (8) formant le couvercle (3) de la boite de pétri (1), afin de rendre impossible la coïncidence des éléments de fermeture respectivement de la

Figure img00020001

grille d'impaction (5) et de l'appareil si le couvercle (3) protecteur du milieu de culture ou gélose (11) de la boite de pétri (1) est présent. It is made in the following manner, this safety device is composed of legs that when closing the grid (5) of the impactor device, the legs (6) must be in place of a part ( 8) forming the lid (3) of the petri dish (1), in order to make it impossible for the closure elements to coincide respectively with the
Figure img00020001

impaction grating (5) and the apparatus if the cover (3) protecting the culture medium or agar (11) of the petri dish (1) is present.

La plupart des grilles d'appareils sont réalisées en aluminium ou en inox pour pouvoir être autoclavable. Most appliance grilles are made of aluminum or stainless steel to be autoclavable.

Notre originalité de conception, notre soucis de la sécurité nous ont dicté un choix différent, nous avons recherché des solutions techniques existant dans d'autres domaines et nous les avons apportées pour les biocollecteurs d'air. Ce choix de matériaux devra ouvrir une porte aux désigners et diminué les coûts de fabrication de ces appareils. Our originality of design, our concern for safety have dictated a different choice, we have researched technical solutions existing in other areas and we have made them for air biocollectors. This choice of materials will have to open a door to designators and reduced the manufacturing costs of these devices.

L'évolution des techniques d'injection plastique et nos recherches sur les différents composants plastiques, fait que notre appareil sera le premier biocollecteur portable à utiliser une grille transparente autoclavable, le choix de la matière est le polycarbonate, car il est transparent et supporte les températures élevées. The evolution of the techniques of plastic injection and our research on the different plastic components, makes that our device will be the first portable biocollector to use a transparent autoclavable grid, the choice of the material is polycarbonate, because it is transparent and supports the high temperatures.

La grille de l'appareil composée en polycarbonate ou d'une autre matière plastique, est un dispositif de sécurité caractérisé en ce que ce composé est transparent, et qu'il permet de visualiser la présence ou non de la boite de pétri pendant le prélèvement. The grid of the device made of polycarbonate or other plastic material, is a safety device characterized in that this compound is transparent, and that it allows to visualize the presence or not of the petri dish during the sampling .

La grille de l'appareil composée en polycarbonate ou d'une autre matière plastique, est un dispositif de sécurité caractérisé en ce que ce composé est transparent et est résistant aux différentes températures d'autoclavage utilisés en hospitalier, c'est à dire

Figure img00020002

autoclavable selon les normes actuelles, c'est à dire 5 minutes à 134Oc. The grid of the device made of polycarbonate or other plastic material, is a safety device characterized in that this compound is transparent and is resistant to different autoclaving temperatures used in hospital, ie
Figure img00020002

autoclavable according to current standards, ie 5 minutes to 134Oc.

La grille utilise pour les trous (7) une technique dite Tube/Plaque en opposition aux appareils dits Fente/Plaque. Auparavant les appareils utilisaient des grilles ou cribles d'une épaisseur faible, 1 à 2 mm maximums. Hors pour une même vitesse de passage à travers le tube que fait le trous dans la grille, la particule sera mieux canalisée à sa sortie et restera le plus longtemps dans l'axe de ce trous que la hauteur de ce trous sera importante. Nos études ont montrées que la hauteur de gélose (9,10) d'une boite de pétri varie en fonction du temps, elle diminue de 1 à 3 millimètres Cette technique permet de compenser la variation de la hauteur de gélose d'une boite de pétri. Cette technique peut être comparée à deux fusils, l'un à canon scié l'autre normal, dans le premier cas le nuage de plomb se disperse avec une grande amplitude, dans le deuxième cas, le nuage de plomb reste groupé et se disperse donc avec une plus faible amplitude pour une même distance d'impact. Le système permet de mieux canaliser les particules, donc de mieux les accélérer perpendiculairement à la gélose (11) et ainsi d'éviter la dispersion des particules les plus légères. The grid uses for the holes (7) a so-called Tube / Plate technique in opposition to devices called Slot / Plate. Previously the devices used grids or sieves of a small thickness, 1 to 2 mm maximum. Out for the same speed of passage through the tube that makes the hole in the grid, the particle will be better channeled at its output and stay longer in the axis of this hole that the height of this hole will be important. Our studies have shown that the agar height (9,10) of a petri dish varies as a function of time, it decreases by 1 to 3 millimeters. This technique makes it possible to compensate the variation of the agar height of a box of kneaded. This technique can be compared to two rifles, one sawed and the other normal, in the first case the lead cloud disperses with a large amplitude, in the second case, the cloud of lead remains grouped and disperses so with a lower amplitude for the same impact distance. The system makes it possible to better channel the particles, thus to better accelerate them perpendicular to the agar (11) and thus to avoid the dispersion of the lightest particles.

L'effet constaté intervient pour un rapport situé de 4 à 8 entre le diamètre des trous et la hauteur des trous, au-dessus de ce rapport les pertes de charges générées deviennent trop importante. La technique d'impaction des particules viables dite Tube/Plaque intervient pour un rapport situé de 4 à 8 entre le diamètre des trous (d) et la hauteur des trous (h), soit h = a X d avec 0, 6 d < 1, 1 mm et 4 a 8 mm. The effect noted is for a ratio of 4 to 8 between the hole diameter and the height of the holes, above this ratio the generated pressure losses become too great. The technique of impaction of the viable particles called Tube / Plate intervenes for a ratio of 4 to 8 between the diameter of the holes (d) and the height of the holes (h), ie h = a X d with 0, 6 d < 1, 1 mm and 4 to 8 mm.

Claims (5)

u 1-Grille d'appareil impacteur adaptable sur une boîte de Pétri, caractérisée en ce qu'elle comporte des trous d'impaction (7) et un dispositif de sécurité permettant à des piétements (6) de se trouver en lieu et place d'une partie (8) formant le couvercle (3) de la boîte de Pétri (1), lors de la fermeture de la grille, afin de rendre impossible la coïncidence des éléments de fermeture respectivement de la grille (5) d'impaction et de l'appareil impacteur, si le couvercle (3) protecteur du milieu de culture de la boîte de Pétri (1) est présent.  u 1-impactor device grid adaptable to a petri dish, characterized in that it comprises impaction holes (7) and a safety device allowing legs (6) to be in place of a part (8) forming the cover (3) of the petri dish (1), when the gate is closed, in order to make it impossible for the closure elements respectively of the impaction gate (5) to coincide with each other; of the impactor device, if the cover (3) protecting the culture medium of the Petri dish (1) is present.
Figure img00030001
Figure img00030001
 REVENDICATIONS 2-Grille (5) d'appareil impacteur, selon la revendication 1, caractérisée en ce qu'elle est en polycarbonate ou d'une autre matière plastique transparente. 2-grid (5) impactor apparatus, according to claim 1, characterized in that it is polycarbonate or other transparent plastic material. 3-Grille (5) d'appareil impacteur, selon la revendication 2, caractérisée en ce qu'elle est résistante aux différentes températures d'autoclavage. 3-Grid (5) impactor apparatus, according to claim 2, characterized in that it is resistant to different autoclaving temperatures. 4-Grille (5) d'appareil impacteur selon l'une des revendications 1 à 3, caractérisé en ce 4-grater (5) impactor apparatus according to one of claims 1 to 3, characterized in that
Figure img00030002
Figure img00030002
 que les trous (7) satisfont l'équation h = a X d, où (d) représente le diamètre trous et (h) représente la hauteur des trous, avec 0, 6 d 1, 1 mm et 4 a < 8 mm.  that the holes (7) satisfy the equation h = a X d, where (d) represents the hole diameter and (h) represents the height of the holes, with 0, 6 d 1, 1 mm and 4 a <8 mm. 5-Utilisation de la grille (5) d'appareil impacteur selon l'une des revendications 1 à 4, pour une technique d'impaction de particules viables dite Tube/Plaque en opposition aux appareils dits Fente/Plaque.5-Use of the grid (5) impactor apparatus according to one of claims 1 to 4, for a viable particle impaction technique known as Tube / Plate in opposition to devices called Slot / Plate.
FR0101574A 2001-02-06 2001-02-06 SAFETY DEVICES FOR THE COLLECTION OF MICROORGANISMS INTO THE AIR Expired - Fee Related FR2820432B1 (en)

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FR0101574A FR2820432B1 (en) 2001-02-06 2001-02-06 SAFETY DEVICES FOR THE COLLECTION OF MICROORGANISMS INTO THE AIR

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2880355A1 (en) * 2004-12-31 2006-07-07 Acanthe Sarl CRIBLE FOR BIO-IMPACTOR, BIO-IMPACTOR EQUIPPED WITH SUCH A CRIBLE
DE102007014082A1 (en) * 2007-03-21 2008-09-25 Sartorius Stedim Biotech Gmbh Filtration unit and method for the microbiological examination of liquid samples
WO2014016607A1 (en) * 2012-07-27 2014-01-30 University Of Newcastle Upon Tyne Autoclable cell culture device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1032574B (en) * 1956-05-02 1958-06-19 Chirana Praha Np Device for determining the number of microbes and other microorganisms in the air
GB1441576A (en) * 1972-09-01 1976-07-07 Secr Defence Air sampling devices
GB2224118A (en) * 1988-08-16 1990-04-25 Burkard Manufacturing Company Air sampler
EP0964241A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling method and sampling apparatus for microbiological analysis of air
JP2000300246A (en) * 1999-04-19 2000-10-31 Midori Anzen Co Ltd Portable type sampler for bacteria floating in air

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1032574B (en) * 1956-05-02 1958-06-19 Chirana Praha Np Device for determining the number of microbes and other microorganisms in the air
GB1441576A (en) * 1972-09-01 1976-07-07 Secr Defence Air sampling devices
GB2224118A (en) * 1988-08-16 1990-04-25 Burkard Manufacturing Company Air sampler
EP0964241A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling method and sampling apparatus for microbiological analysis of air
JP2000300246A (en) * 1999-04-19 2000-10-31 Midori Anzen Co Ltd Portable type sampler for bacteria floating in air

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 13 5 February 2001 (2001-02-05) *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2880355A1 (en) * 2004-12-31 2006-07-07 Acanthe Sarl CRIBLE FOR BIO-IMPACTOR, BIO-IMPACTOR EQUIPPED WITH SUCH A CRIBLE
WO2006072691A1 (en) * 2004-12-31 2006-07-13 Acanthe Sieve for bio-impactor, bio-impactor equipped with same
DE102007014082A1 (en) * 2007-03-21 2008-09-25 Sartorius Stedim Biotech Gmbh Filtration unit and method for the microbiological examination of liquid samples
DE102007014082B4 (en) * 2007-03-21 2009-04-02 Sartorius Stedim Biotech Gmbh Filtration unit and method for the microbiological examination of liquid samples
US8951788B2 (en) 2007-03-21 2015-02-10 Sartorius Stedim Biotech Gmbh Filtration unit and method for the microbiological analysis of liquid samples
WO2014016607A1 (en) * 2012-07-27 2014-01-30 University Of Newcastle Upon Tyne Autoclable cell culture device

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Effective date: 20111102