FR2780721A1 - BIOLOGICAL MEANS OF SANITATION OF POLLUTED EFFLUENTS AND / OR MAINTENANCE OF THE DRAINAGE OF THE SAID EFFLUENT - Google Patents

Abstract

The invention concerns biological means for sanitary drainage of polluted effluents and/or maintenance of a sewer pipe system for evacuating said effluents. One of the aims of the invention is to offer bacteriological means for pollution management and sanitary drainage not only of soiled effluents but also of the infrastructure evacuating them and which contains them, said means needing to be simple, economical, non-pathogenic and efficient. The invention achieves this as it concerns a biological means for sanitary drainage of polluted effluents and/or maintenance of the sewer pipe system evacuating said effluents, characterised in that it comprises at least a biodegradable support loaded with a composition of saprophytic bacteria belonging, preferably to at least one of the following species Bacillus subtilis, Rhodococcus rubica, Rhodococcus rubra, Pseudomonas putida, Pseudomonas putrefaciens. Said means is advantageously bioactive toilet paper loaded with bacteria of these five species in three bacterial floras F1, F2, F3 respectively adapted for the biodegradation of the support, maintenance of the pipe system and pollution management.

Description

The field of the present invention is that of the bioprocessing of

  polluted effluents of domestic origin and / or from industry and / or agriculture.

  By bioprocessing is meant the use of living microorganisms capable of transforming, by metabolization, organic or even mineral matters which contaminate all the liquid discharges produced at home, as well as in industry and

in agriculture.

  The present invention relates to a biological means of this type, active in the remediation of polluted effluents and / or in the maintenance of pipelines.

evacuation of said effluents.

  The invention also relates to the bacterial active ingredient, in the form

  solid or liquid, forming part of the above-mentioned means.

  Furthermore, the invention relates to a method for preparing the means

biological considered.

  The bacteriological treatment of polluted effluents, and in particular the elimination of fats and hydrocarbons contained in these effluents, has been known for a very long time. But if, in principle, this means of depollution seems attractive and interesting, it turns out in practice that it is not as efficient as one might have wished. This is partly related to the fact that the biological active material remains difficult to handle. In addition, the customs and culture in terms of depollution, in particular biological, are still little developed. We therefore know, for example, that 8 out of 10 urban effluent treatment plants show more or less malfunctions.

less serious.

  To the extent that the regulatory framework concerning respect for the environment will become increasingly drastic, it is important that everyone has, individually, all the information and all the desirable means

  to promote and facilitate the biological fight against pollution.

  The poor management of the requirements of the biotreatment of the contaminated effluents that we produce and that our industry and our agriculture produce, also has repercussions in the maintenance of the infrastructure for the collection and disposal of wastewater. Indeed, with regard to the sanitation and maintenance of sewage pipes and columns in buildings, we are faced with a problem which has not, to date, been addressed. satisfactory solutions. In practice, fats, organic matter, fatty scales or other similar materials, form concretions in the light of the pipes. So we are gradually witnessing a clogging of these pipes which causes, among other things, backflow and release of bad odors. To remedy this, but only curatively and not preventively, we conventionally proceed to a mechanical cleaning operation of the interior of the pipes. This technique is expensive and must be renewed regularly. In addition, it does not restore the qualities of the material of the pipe walls, hence a risk of cracking and appearance

  subsequent leaks increasing continuously as they wear out.

  To generally optimize the biotreatment of polluted effluents and to try to provide a solution to the problem of maintenance of pipelines and related clogging, the field of biotechnology has proposed bacteriological means judiciously constructed and selected for this purpose. There are thus liquid or pulverulent bacterial compositions intended to regulate and standardize the functioning of purification sites such as septic tanks, treatment basins and lagoons, or even grease traps. These compositions contain suitable bacteria, whether in lyophylized form or not, as well as

  suitable enzymes, mineral salts, trace elements and nutrient agents.

  These compositions have the effect of reactivating the flora of the purification medium. They also help to eliminate bad odors. They promote and accelerate the transformation of organic matter and contribute to the liquefaction of crusts

of polluting material.

  In the same way, there are on the market bacterial compositions also called purifying aids for the maintenance of pipes for discharging polluted effluents. These compositions have an action of biodegradation of fats and organic matter. They allow the separation of fatty tartars or other concretions (limestone, silica, phosphate ...). In addition, the flora brought by these compositions neutralize the harmful effects of the commensal flora of the pipes, in particular with regard to the formation of H2S which, in addition to the olfactory nuisances which it causes, has a corrosive action on the walls of the pipes. . In summary, these compositions make it possible to combat the clogging of the pipes. They constitute a veritable biological means of cleaning and sanitation which supplements, or even advantageously replaces the means

  traditional maintenance (chemical and / or mechanical).

  By nature, these powders and biotreatment liquids underlie a slightly restrictive application methodology which requires a minimum of training. In such a context, one of the motivations of the inventor was to move in the direction of maximum simplification for the implementation of means

  depollution and sanitation bacteria.

  Another essential objective which presided over the inventive approach specific to the present invention, was to bring into play notions of regularity and low dose,

  which are essential in microbiology.

  Another fundamental objective of the invention was to take into account the economic constraint by reflecting on the development of means of

  bioprocessing as simple as inexpensive.

  Another essential objective of the present invention was naturally to integrate the non-pathogenicity parameter of the flora used in the

biological means sought.

  Another essential objective of the invention was to develop

  adapted associations of specific and infraspecific bacteria.

  Another objective of the invention consisted in developing a simple and economical and safe preparation process for a biological means of purification.

  presenting the above-mentioned specifications.

  Having set these objectives, the inventor had the merit of developing, in a completely surprising and unexpected manner, after long and laborious research and experiments, a biological means of sanitation of polluted effluents and / or maintenance of the evacuation pipes of said effluents, characterized in that it comprises at least one biodegradable support loaded with a composition of saprophytic bacteria preferably belonging to at least one of the following species: Bacillus subtilis Rhodococcus rubica Rhodococcus rubra Pseudomonas putida

Pseudomonas putrefaciens.

  The invention proceeds in the first place from the original and advantageous selection of several bacterial species endowed with metabolic faculties adapted to the transformation of polluting substrates contained in domestic effluents or coming from industry and agriculture and having moreover aptitudes to fight against concretions clogging the pipes or other ways

  disposal, such as sewers.

  In addition to this bacterial selection, the invention is also based on the choice of a biodegradable support, preferably by the active bacteria which it contains. It is a question of implementing a vector capable of ensuring the transport of this active living matter where it is intended to exert its action, that is to say in the evacuation pipes and in the purification sites. such as septic tanks, sewage tanks, lagoons, grease traps, etc. As a corollary, this vector must not be a pollution factor and it is therefore provided in accordance with the invention that this support is bioresorbable by the microorganisms present in the media considered and, preferably, by the microorganisms of the

  bacterial composition selected according to the invention.

  In accordance with a preferred embodiment of the invention, the bacterial composition comprises: - at least one flora F1 of bacteria adapted to the biodegradation of the support, - and / or at least one flora F2 of bacteria suitable for cleaning and maintenance of the evacuation pipes of polluted effluents, - and / or at least one flora F3 of bacteria adapted to the depollution in purification sites of the septic tank type, basin / lagoon

sewage treatment plant or grease trap.

  The F1 flora can be composed of one or more different strains belonging to one or more of the 5 species of bacteria selected in the context of the invention. This flora F, removes the pollutant load that constitutes

  potentially the carrier of the bacterial composition.

  The bacteria forming the flora F2 can also present a

  infra-specific variability and belong to one or more of the 5 selected species.

  This F2 flora is specialized in the fight against clogging of drainage pipes. Finally, the flora F3 has the same characteristics as the flora FI and F2 except as regards its exacerbated aptitude for promotion and improvement.

  the functioning of liquid biological purification media.

  Advantageously, the microorganisms used in the means according to the invention are not pathogenic. These are class 1 bacteria in accordance with standard NF X42-040. This class I which corresponds to microorganisms which have never been described as causative agents of

  diseases in humans that do not pose a threat to the environment.

  Nor can these class I non-pathogenic bacteria be considered opportunistic pathogens. Given this nonpathogenicity, the bacteria of the composition can be brought into contact with any part of the body

  human and in particular with the skin, even if it is damaged.

  It is of definite technical and commercial interest that the biological means according to the invention be provided with a bacterial composition comprising simultaneously an F1 flora, an F2 flora and an F3 flora, in order to increase these potentials. In the same vein, it is recommendable that the composition

  bacterial contains bacteria belonging to each of the 5 species mentioned above

  above, namely: subtle Bacillus, Rhodococcus rubica, Rhodococcus rubra,

  Pseudomonas putida and Pseudomonas putrefaciens.

  The variety and infraspecific variability of the bacteria in the composition is one of the strong points of the biological means according to the invention. Indeed, the fact that the species (or species) of the bacterial composition includes (s) different strains within the same species, makes it possible to build a tailor-made biological tool to

  broad spectrum, suitable for different classes of polluting substrates.

  To further perfect, without this being limiting, the identification and definition of the bacteria associated in the means according to the invention, we give below, on the one hand, the morphological and physiological characteristics and, on the other hand , the

  biochemical characteristics of 5 bacterial species mentioned above.

  I - Subtle Bacillus: 1.1. Morphological and physiological characteristics GRAM rods + non-deformable oval spore with thin wall catalase + aerobic mobile strict growth temperature: 10-50 degrees C optimal growth temperature: 25-35 degrees C. 1.2. Biochemical characteristics acetone production (VOGES PRAUSKAUER test) VP + starch * acid on glucose +

indole-

casein + golatin + lecithin +

urease-

  nitrate +. 2 - Rhodococcus Rubica: 2.1. Morphological and physiological characteristics group of bacteria Actinomycetales (complex identification) family of Nocardioforms variable morphology, limited filamentation, morphogenetic cycle with cocciform or bacillary cells

GRAM +

  2.2. Biochemical characteristics Arabinose + Galactose + Cellulose + Starch + 3 - Rhodococcus Rubra: 3.1. Morphological and physiological characteristics group of bacteria Actinomycetales (complex identification) family of Nocardioforms variable morphology, limited filamentation, morphogenetic cycle with cocciform or bacillary cells GRAM + 3.2. Biochemical characteristics Arabinose + Galactose + Cellulose + Starch +. 4- Pseudomonas Putida: 4.1. Morphological and physiological characteristics

GRAM- sticks

  aerobic oxidase + catalase + PHB acid synthesis 4.2. Biochemical characteristics

Nitrate-

indole'

glucose fermentation-

  ADH + urea- glucose gelatin + ARA + MNE +

MAN +

  CAP + MLT + CIT + OX +. 5 - Pseudomonas Putrefaciens: 5.1. Morphological and physiological characteristics

GRAM- sticks

  aerobic oxidase + catalase + PHB acid synthesis 5.2. Nitrate + biochemical characteristics

indole-

  glucose fermentation ADH- urea- esc + gelatin + glu 'ARA MNE- MAN- CAP +

MLT +

  CIT- oX +. Regarding their origins, the bacteria used are

  advantageously natural and have not been subject to any genetic manipulation.

  They can be, for example, in practice commercial bacteria present either in vegetative form or in spore form. Conventionally, these commercial bacteria are kept in anhydrous form, by

lyophilized example.

  Preferably, the bacterial composition also includes suitable nutrients and / or enzymes. As other adjuvants which can be incorporated into the formulation including the above-mentioned bacterial composition, it is possible to

  cite mineral salts, trace elements.

  All these adjuvants are judiciously chosen by a person skilled in the art to reactivate revivify (awaken) the bacteria which must pass from the vegetative or spore form to a living form of growth. Nutrients, enzymes or other mineral salts and trace elements are intended to place bacteria in the exponential growth phase. Correctly chosen, this growth environment also makes it possible to select particular phenotypes, at the origin of precise metabolic functionalities appropriate to the role sought for these bacteria. In practice, it may be indicated, by way of example, that the bacteria used are lyophilized bacteria conditioned on a support of the lithotamous or bran powder type and accompanied by awakening and protective nutrients to enable them to resist treatment. lyophilization (eg: nitrogen,

phosphorus, trace elements ...).

  For the use of these bacteria in the means according to the invention, the formulation comprising the bacterial composition can optionally be further

  added with other nutrients, enzymes, trace elements and specific mineral salts.

  Furthermore, the bacterial composition may include natural compounds secreted by the bacteria themselves. As examples of these compounds

  endogenous, there may be mentioned biosurfactants or other lipases.

  With regard to the support which is the other essential characteristic element of the invention, it is very particularly advantageous that it is of cellulose nature. Paper supports are preferred but, better still, paper supports

  paper made of cellulose wadding is especially preferred.

  Thus, it is when the support consists of toilet paper and / or hygienic, that the biological means according to the invention best expresses all of these

  potential and all these advantages.

  Indeed, a toilet paper loaded with an infra and / or plurispecific bacterial association (subtle Bacillus, Rhodococcus rubica, Rhodococcus, rubra, Pseudomonas putida and Pseudomonas putrefaciens) constitutes a simple means,

  economical and efficient biological pollution control.

  Advantageously, the toilet paper according to the invention can comprise: a flora F1 of bacteria capable of degrading the cellulose and starch derivatives, and / or an flora F2, always originating from the same species, the latter being however more specially selected in with a view to ensuring the maintenance and unclogging of the pipes, these bacteria F2, being particularly suitable for the biodegradation eg of fats and starches as well as for the neutralization of the sulfur cycle; - and / or a flora F3 of bacteria suitable for the biotreatment of polluted effluents at the level of treatment sites which can be septic tanks, treatment tanks, grease traps, lagoons, etc. The toilet paper loaded with the above-mentioned bacterial composition can be likened to a means of seeding, by bacteria capable of ensuring

  sanitation, of the entire drainage and purification network for contaminated effluents.

  The systematic and regular use of toilet paper is perfectly suited to the specifications required in terms of biological purification. Indeed, in this area, it is preferable to inoculate the purification medium with small regular doses of bacteria rather than to carry out a weekly inoculation in large doses, likely to be destroyed in the event of the appearance of factors d 'inhibition

in the environment (eg. chlorine).

  The fact of using toilet paper as a seeding medium is all the more attractive as it does not impose any methodological constraints and allows everyone to be an actor in promoting the biological purification of

polluted effluents.

  According to a preferred characteristic of the invention, the biological sanitation means is formed by a toilet paper support loaded with the above-mentioned bacterial composition, at a rate of at least 104, preferably, at a rate of from 107 and , more preferably still, at a rate of approximately 106 bacteria / g dry toilet paper. It is thanks to bacterial loads of this order of magnitude that the paper according to the invention allows the whole network to be sown with a sufficient number of exogenous saprophytic bacteria to compete with the endogenous bacteria present in the media to be treated. : pipes - sewers - treatment sites. This is how colonization and the expression of functions operate.

  sought after sanitation and biological maintenance.

  According to another of these aspects, the present invention relates to the bacterial composition, adjuvanted or not, used in the means

  biological as described above.

  This bacterial composition can be in powdery solid form or else in liquid form, for example in the form of a suspension, preferably aqueous, comprising the above-mentioned bacterial composition as a heterogeneous solid phase. It is preferable that the bacterial composition is in liquid form and in particular in the form of a suspension, with a view to its application to the support, by

  example in cellulosic material such as toilet paper and / or toilet paper.

  With regard to the preparation of biological sanitation means as defined above, the present invention also relates to a process for the preparation of said means, characterized in that it essentially consists: - possibly of awakening (revivifying) and / or reactivating bacteria of the bacterial composition,

  - to use a liquid suspension - preferably aqueous -

  containing the bacterial composition, preferably supplemented with nutrients and / or enzymes and / or mineral salts and / or trace elements; bringing said active bacterial suspension into contact with the biodegradable support, preferably in toilet and / or hygienic paper; - and to ensure that the liquid phase of the suspension is eliminated,

preferably by evaporation.

  According to an advantageous arrangement of the invention, the cellulosic support,

  preferably the toilet paper used is an embossed paper.

  Insofar as the bacterial strains used are inactive, because in vegetative or spore form, it is necessary to provide a prior step of revivification and multiplication of the bacteria considered in an appropriate culture medium, comprising nutrients in the form of carbon source and / or nitrogen and / or phosphorus, enzymes of the trace elements and / or specific mineral salts. This preliminary stage consists in a culture in a bioreactor which must lead to concentrations of active bacteria of at least 10 10 bacteria / ml of liquid culture medium. Naturally, this culture takes place under controlled pH and temperature conditions. The culture medium can be supplied with nutrients as and

  as these are consumed by the growing bacteria.

  Concentration analyzes on certain substrates of the culture medium are carried out regularly or continuously. Similarly, we proceed to an enumeration

  bacterial, in order to follow the evolution of the growth of the strains.

  Naturally the quality of the water used in the culture medium is also controlled. This possible preliminary stage of culture for the purpose of revivifying inactive bacteria (for example lyophilized) also constitutes a means of infraspecific selection for all or part of the bacterial species used in the

means according to the invention.

  It follows that according to a preferred arrangement of the invention falling within the preferred embodiment where three types of flora F, F2 and F3 intervene as defined above, the selection of these specific flora is carried out by culturing separate selective to obtain 3 mother suspensions each containing Fl,

  F2 and F3, which will then be mixed before being applied to the support.

  In accordance with the invention, each mother suspension of specialized flora F1, F2 or F3, each having an infraspecific variety and a targeted spectrum of activity, is obtained, more preferably still, by implementing the respective respective broths Flora F .: A Rhodococcus rubica A Rhodococcus rubra A nutrients, preferably of peptide nature - Flora F2: A Bacillus Subtilis A Pseudomonas Putida A Pseudomonas Putrefaciens A nutrients, preferably of peptide nature - Flora F3: A Bacillus Subtilis A Pseudomonas Putida A Pseudomonas Putrefaciens A Rhodococcus rubica

  Nutrients, preferably peptide in nature.

  This preferred configuration of the flora broths Fi, F2, F3 corresponds firstly to an interspecific selection, and secondly to an induction of infraspecific mutations leading to strains specialized in

  metabolizations assigned to the bacterial composition of the means according to the invention.

  It should be noted that the present invention also relates, as a new product, to a bacterial composition containing the flora F1, F2, F3 as defined above by the properties of the bacteria which they comprise (cf. page 4 lines 13 to 19 ) and by

  their production characteristics (see page 11 line 23 to page 12 lines 1 to 6).

  It should be observed that the activated bacterial suspension after culture of revivification and multiplication must advantageously be fixed on the support within a limited time, for example one hour, due to the exhaustion of the nutrient resources. Once an active bacterial suspension is available, the bacterial composition is applied and / or brought into contact, preferably adjuvanted in nutrients / enzymes / trace elements / mineral salts, by coating and / or

  misting and / or spraying, preferably by coating.

  The latter technique appears to be more advantageous on the industrial level, because it limits the risks of contamination and bacterial spread in production premises, notwithstanding the non-pathogenicity of the bacteria used.

  in the composition specific to the invention.

  The coating methods are well known in the field of paper processing. It may for example be devices in which the strip of paper to be coated is passed through the air gap of two rotary rollers, one of which (at least) is supplied with a film of coating liquid (size press- gate roll press). We

  may also consider scraping techniques.

  In this case, the coating liquid consists of the activated bacterial suspension,

  adjuvanted and kept stirring.

  In practice, the suspension to be applied contains, for example, around 1 g of dry matter (bacteria + excipients) per liter. The coating load is approximately 200 ml of suspension per kilo of paper or approximately 7 ml / m2 of paper, knowing that the density

  seeding should preferably be at least 106 / g of paper.

  Advantageously, the culture broth is in the exponential growth phase with a concentration of bacteria of approximately 1010 bacteria / ml, before sowing on the support, for example, by coating, spraying or misting. The last step in the process for preparing biological sanitation means according to the invention is that of total or partial evaporation of the aqueous liquid phase, which has allowed the transport and deposition on the support,

  preferably in toilet paper, the adjuvanted bacterial composition.

  According to an optional variant, it is conceivable to provide drying means in order to facilitate the elimination by evaporation of this aqueous phase. However, it goes without saying that evaporation into the ambient atmosphere can be more than sufficient. As we have understood, one of the major but not exclusive applications of biological means for the remediation of polluted effluents and / or for the maintenance of the evacuation pipes for said effluents, as defined above by characteristics of structure and obtaining, is that of toilet paper

and / or bioactive hygienic.

  Such papers loaded with this judiciously selected bacterial composition are intended to circulate and to be present in the evacuation and treatment networks of domestic polluted effluents, even those from industry or agriculture. Non-pathogenic, economical, simple to use and efficient in sanitation and maintenance of the evacuation network, this means concentrates many advantages and brings great technical progress in the field of

biological purification.

  As examples of other biological means of sanitation different from toilet paper loaded with bacteria, there may be mentioned paper towels, sanitary napkins, tissues, papers

"wipe

all "....

  The invention will be better understood with the aid of the example which follows and which also has the advantage of clearly highlighting all the advantages and the variants.

for carrying out the invention.

EXAMPLE

  1 - Bacterial material The strains used in this example are Bacillus subtilis, Pseudomonas

  putida, Pseudomonas putrefaciens, Rhodococcus rubica and Rhodococcus rubra.

  The morphological, physiological and biochemical identity card of these 5 species of

  bacteria is given on pages 5, 6 and 7 of the description.

  These bacteria are available in lyophilized form commercially, under the references: - ACTIFORCE piping (ACTION BIO company) ACTIFOSSE L (ACTION BIO company) - EUROBIO EC (EUROBIO company) - EUROBIO FS (EUROBIO company)

- EUROBIO CELL (EUROBIO company).

  In this example, the 9 commercial strains all produced by the company EUROBIO and listed below are more specifically retained: - 1 - Bacillus subtilis EBl - 12 - Bacillus subtilis EB2 - 21 Rhodococcus rubica EBl - 22 - Rhodococcus rubica EB4 - 3 - Rhodococcus rubra EB3 - 41- Pseudomonasputida EB7 - 42 - Pseudomonas putida EB14 - 51 Pseudomonas putrefaciens EB 1 - 52 - Pseudomonasputrefaciens EB5 2 Culture of revivification / reactivation and selective multiplication of the 9 commercial strains (Ij. 12, 222, 3, 41 42,51 . 52) referred to in 1 for the production of the flora F, _ F2 F3 Each of the Flora F1, F2, F3 is produced individually in a 1 liter fermenter containing a rich liquid medium constituted by a broth containing casein peptone and flour soy. This medium is of the type marketed by the

  MERCK or that marketed by DIFCO.

  The commercial strains used in the fermenter of

  production of flora F, are strains 2, and 3.

  The commercial strains used in the fermenter of

  production of F2 flora are strains 12,41, 52.

  The commercial strains used in the fermenter of

  production of F3 flora are strains 11, 42, 51, 22.

  The initial concentration of bacteria in each of the three fermenters is approximately 1010 bacteria / ml of medium. The pH of the culture medium inside the fermenters is neutral and its temperature is 30 C. After stirring for a few hours, a broth of

  culture and a concentration of bacteria of the order of 1010 / ml.

  The bacteria are counted on standard nutritive gelose

  (PCA): (Agar with casein peptone, glucose and yeast extract).

  3 - Inoculation of the cellulosic support The 3 cultures obtained as described in point 2 above, are mixed in the same proportions in order to produce a bacterial suspension by means of which the inoculation of the support constituted by toilet paper will be carried out.

  marketed by PERLA SPA under the registered trademark LORY.

  Ten sheets of this 12.5 x 10 cm toilet paper with two folds, each having a density of 17 g / m2, are impregnated with the suspension

  bacterial using a fogger.

  This impregnation is carried out at the rate of 200 μl of suspension per gram of

  paper, i.e. 850 pl for 10 sheets, whose total mass corresponds to 4.25 g.

  4 - Verification of the colonization of the support by the bacteria After drying in a sterile storage for 1 week, the paper is taken up

  and ground according to conventional techniques of food microbiology.

  The grinding broth is seeded on: - PCA agar (total flora) tributyrin agar (lipase flora: B Subtilis)

  - Actinomycetes special agar, type DIFCO 0957-17-4 (Rhodococcus).

  - TOTAL FLORA results (Bacteria / g)

TEST 1 7.105

TEST 2 1,1.106

TEST 3 2.105

  Good lipase activity of the colonies on tributyric gelose.

  Global activity close to the conditions on traditional supports already marketed

(lithotham, zeolite, sound ...).

  6 - Variant In this variant, the same bacterial suspension is used, but the sprayer is replaced with a roller press device of the size press type. The

  volume of suspension deposited by coating is 200 ml / kg.

  Like what happens after the misting implemented in point 3 above, the removal of the water fixed on the toilet paper holder is carried out by

evaporation to ambient air.

  The same results are obtained by coating as by misting,

  terms of bacterial colonization.

  7 - Application The toilet paper loaded with bacterial composition obtained according to the present example, are introduced into the network for evacuation of domestic polluted effluents, by means of a toilet bowl. It can be seen that no regular introduction, over several months, of seeded toilet paper, leads to limiting the formation of concretions in the evacuation pipes and allows the improvement of the performance of the treatment site in which the effluents are treated. by said pipeline. In addition, there is also a

  notable digestion of the cellulosic support carrying the active sanitation flora.

Claims (9)

CLAIMS:   1 - Biological means for cleaning up polluted effluents and / or for maintaining the evacuation pipes for said effluents, characterized in that it comprises at least one biodegradable support loaded with a composition of saprophytic bacteria preferably belonging to at least one of the following species: Bacillus subtilis Rhodococcus rubica Rhodococcus rubra Pseudomonas putida Pseudomonas putrefaciens.   2 - Means according to claim 1, characterized in that the bacterial composition comprises: - at least one flora F, of bacteria adapted to the biodegradation of the support, - and / or at least one flora F2 of bacteria suitable for cleaning and maintenance of the evacuation pipes for polluted effluents, - and / or at least one F3 flora of bacteria suitable for pollution control in treatment plants.   3 - Means according to claim I or 2 characterized in that the bacterial composition comprises bacteria belonging to each species mentioned in claim 1.   4 - Means according to any one of claims 1 to 3, characterized in   what (or the) species of the bacterial composition includes (including)   different strains within the same species.   - Means according to any one of claims 1 to 4, characterized in   what the bacterial composition also includes nutrients and / or enzymes.   6 - Means according to any one of claims 1 to 5 characterized in   what the support is of a cellulosic nature, paper being preferred and paper being   cellulose wadding being especially preferred.   7 - Means according to any one of claims I to 6, characterized in   that the support consists of toilet paper and / or toilet paper.   8 - Bacterial composition used in the means according to one   any of claims 1 to 7.   9 - Bacterial composition according to claim 8, characterized in that it comprises: - at least one flora F1 of bacteria adapted to the biodegradation of the support, this flora being obtained by culturing the bacteria Rhodococcus rubica and Rhodococcus rubra in a broth additionally containing nutrients, preferably of a peptide nature, and at least one flora F2 of bacteria suitable for cleaning and maintaining the pipes for discharging polluted effluents, this flora being obtained by culturing the subtle Bacillus bacteria, Pseudomonas putida and Pseudomonas putrefaciens, in a broth additionally containing nutrients, preferably of peptide nature, - and at least one flora F3 of bacteria suitable for depollution in purification sites, this flora being obtained by culturing bacteria Bacillus subtilis, Pseudomonas putida, Pseudomonas putrefaciens and Rhodococcus rubica, in a broth containing out re   nutrients, preferably peptide in nature.   - Bacterial suspension, preferably aqueous, characterized in that   that it comprises the composition according to claim 8 or 9.   11 - Method for preparing the means according to any one of   claims 1 to 7, characterized in that it consists essentially in setting   contacting the active bacterial suspension according to claim 9 with the biodegradable support, and ensuring that the liquid phase of the suspension is eliminated, preferably by evaporation.