FR2780283A1 - Inhibiting microbial growth in preservative-free aqueous cosmetic composition - Google Patents

Abstract

A combination of a diol (I) containing 3-4 carbon atoms and a sequestrant (II) is used to inhibit microbial growth in a preservative-free aqueous cosmetic composition. An Independent claim is also included for preservative-free aqueous cosmetic composition containing 0.5-15 wt. % (I) and 0.01-0.5 wt. % (II).

Description

The present invention relates to an aqueous cosmetic composition without

  preservative containing a diol compound in C3-C4 in combination

with a sequestering agent.

  Conventionally, cosmetic compositions are preserved from microbiological contamination due to the development of microorganisms introduced into cosmetic compositions, either during their manufacture, or even during their use, by raw materials qualified as "preservatives" and which form part of a positive list (Annex IV of the Directive

  o10 European 76/768 / EEC modified from August 1997).

  However, if these preservatives solve the problems of contamination, they are also the cause of other problems. They are particularly likely to cause allergies, and to be responsible

  skin and eye irritations.

  To overcome the disadvantages of these preservatives, as well as to meet consumer demand for "natural" products,

  so-called "preservative-free" cosmetic compositions have been developed (J.J.

  Kabara and D.S. Orth, Preservative-free and self-preserving Cosmetics and Drugs,

Marcel Dekker, 1997).

  In the case of aqueous cosmetic compositions, said “preservative-free” cosmetic compositions in fact exhibit a self-preservation system thanks to the physicochemical properties of the envisaged formulation. Thus, low water (aw) activity, acidic or basic pH, or agents known for their antimicrobial properties, such as alcohols, surfactants, or quaternary ammonium compounds, prevent or prevent the development of microorganisms in said cosmetic compositions.

  Throughout the description below, the term “

  the expression "cosmetic composition without preservative" a cosmetic composition containing none of the raw materials from the list in the appendix

  IV of the modified European Directive 76/768 / EEC of August 1997.

  The authors of the present invention have discovered that the combination of a hydrating agent chosen from C3-C4 diols and a sequestering agent makes it possible to prevent the development of microorganisms in

  aqueous cosmetic compositions without preservative.

  The present invention therefore relates to the use of a combination of a hydrating agent chosen from C3-C4 diols and a sequestering agent in an effective amount, in an aqueous cosmetic composition without preservative, to prevent the development of microorganisms in

said cosmetic composition.

  More particularly, said hydrating agent can be used at a content of 0.5 to 15% by weight of the cosmetic composition and said sequestering agent can be used at a content of 0.01 to 0.5% by weight of the cosmetic composition.

cosmetic composition.

  A more particular subject of the present invention is an S5 aqueous cosmetic composition without preservative containing: - from 0.5 to 15% by weight of a moisturizing agent chosen from C3-C4 diols alone or as a mixture, and

  - from 0.01 to 0.5% by weight of a sequestering agent.

  The content of said hydrating agent in the cosmetic composition according to the present invention may preferably be between 0.5 and 5%, of

  preferably still between 3 and 4.5%.

  Said hydrating agent can be in particular propylene glycol, butylene glycol or 2-methyl 1,3-propanediol (marketed under the name of MP

  Diol Glycol by Arco Chemical Compagny).

  Said moisturizing agent chosen from C3-C4 diols can be used alone or as a mixture. Preferably, said hydrating agent is

  2-methyl 1,3-propanediol, used alone or as a mixture with other C3- diols

  C4, such as in particular propylene glycol.

  The content of said sequestering agent may preferably be

  between 0.1 and 0.4% by weight.

  Said sequestering agent can in particular be a salt of EDTA,

  preferably tetrasodium EDTA.

  Said cosmetic composition according to the invention can also

contain an emulsifying agent.

  The content of said emulsifying agent can be in particular

  between 0.1 and 5%, preferably between 0.3 and 1%.

  s The said emulsifying agent may in particular be an acid derivative

  fat containing 6 to 14 carbon atoms.

  By "fatty acid derivative" is meant in particular fatty acid esters, such as glycerol monolaurate, alkali metal salts, such as sodium laurate, or amine salts. Preferably, said derivative

  1o of fatty acid chosen is sodium laurate.

  A preferred cosmetic composition according to the invention is an emulsion containing 2-methyl 1,3-propanediol, tetrasodium EDTA and

sodium laurate.

  Preferably, the content of tetrasodium EDTA is 0.1%, and 2-methyl 1,3-propanediol and sodium laurate are used in a

  0.9 / 0.1 2-methyl 1,3-propanediol / sodium laurate ratio.

  Another preferred cosmetic composition according to the invention is an emulsion containing 2-methyl 1,3-propanediol, tetrasodium EDTA and glycerol monolaurate. Preferably, the tetrasodium EDTA content is 0.1%, and 2-methyl 1,3-propanediol and glycerol monolaurate are used in a 2-methyl 1,3propanediol / monolaurate ratio.

0.9 / 0.1 glycerol.

  The following examples illustrate the invention without limiting it.

  Example 1 Effectiveness of a Cosmetic Composition Containing 2-methyl 1,3-Propanediol in Combination with EDTA

  tetrasodium to prevent microbiological contamination.

  A. Preliminary test: a) Preparation of the tested formulation: In order to be able to analyze the effectiveness of 2-methyl 1,3-propanediol in preventing the development of microorganisms within a cosmetic composition containing tetrasodium EDTA, the authors of the present invention used a body milk formula as described in Table I.

Table 1:

FIRST QUANTITY MATERIAL PHASE

  Triethanolamine 4 g Na4 EDTA 0.125 g A Propylene glycol 25 g Allantoin 0.5 g Demineralized water 215.825 g Lanolin alcohol 1.5 g Vaseline 8.5 g B Glycerol stearate 0.750 g Stearic acid 17.5 g Cetyl alcohol 0.5 g Mineral oil 35 (Marcol 82, 34 g Esso Chimie) C Cyclomethicone 10 g D Water 150 g Carbopol D 981 (BF Goodrich) 0.5 g E Mineral oil 35 (Marcol 82, 2 g Esso Chimie) Demineralized water 24, 5 g Triethanolamine 0.6 g G Demineralized water 0.7 g The operating conditions for the manufacture of this body milk are as follows: - Prepare the gel for phase E: Disperse Carbopol 981 well in mineral oil 35; Shake the cold water (pH: 5) vigorously but without a vortex;

  Introduce the oily polymer dispersion.

- Prepare the emulsion:

  In components, introduce components A, heat to 80 C-

  s 85 C by stirring with a turbine (RAYNERY) (1000 rpm), with a

thermostat hot plate 5.

  Successively introduce the components of phase B. Maintain the emulsion at a temperature of 800C for 30 minutes, at a speed of

1000 turns.

  Add the silicone at 1,700 rpm.

  Introduce cold water at 1000 rpm.

  At about 60 C, add the gel, stir with a turbine around 2000

rpm for 10 minutes.

  Then switch to paddle stirring (HEIDOLPH) at 1000 rpm

  at 35 C. Add phase G. Stop at 30 C.

  The pH is measured at room temperature and if necessary the medium

  is acidified with hydrochloric acid.

  To this formulation are added 7% of 2-methyl 1,3-propanediol.

  b) Results: The influence of 2-methyl 1,3-propanediol has been studied with respect to contamination by Aspergillus niger. The difference in log CFU / ml between D0 and D14 (CFU: Colony-forming unit) is -2.9 for a concentration of

7% 2-methyl 1,3-propanediol.

  B. Study of the effectiveness of a composition containing 2-

  methyl 1,3-propanediol and r'EDTA in combination with additional agents: a) Preparation of the tested formulation: To be able to analyze the effectiveness of 2-methyl 1,3-propanediol in combination with additional agents to prevent development of microorganisms within a cosmetic composition, the authors of the present invention used a body milk formula as described in

the table II.

Table II:

  Raw material Quantity Phase (in%) Ethoxylated fatty alcohol (Arlatone 985) 4 A Polyoxyethylene stearyl ester 21 (Brij 721) 2 A Heptamethylnonane (Arlamol HD) 10 A Sorbeth 30 (Atlas G2330) 3 B Water qs B Conservative mixture see table C III The operating conditions for the manufacture of this body milk are as follows: The two phases A and B are heated to 75 C and mixed with the turbine (1200 revolutions / minute) for 20 minutes at a temperature above 0C. At 60 ° C., phase C is added to the emulsion. Speed

  stirring at 1,200 rpm is continued for another 5 minutes.

  Then the emulsion is transferred to a paddle mixer (500

  rpm) until cooling.

  The system is agitated until cooling by means of a blade 4

propellers at 500 rpm.

  The preservative mixtures have the formula (Table III).

Table II1:

  Reference MPDiol EDTA Propylene Glycerol monolaurate Glycol sodium laurate% (Na4) glycol%% formula%%

34 4.5 0.1 0 0.5 0

4.5 0.1 0 0 0.5

38 2.50 0.1 2.50 0 0

62 2.50 0 2.50 0 0

63 4.50 0 0 0.5 0

b) Results:

  The results are reported in Table IV below.

Table IV:

  Reference of E. coli S. aureus P. aeruginosa C. albicans A. niger the formula (log CFU / ml) (log CFU / mI) (FCm) og CFU / ml) (log CFU / ml)

OJ 5.1 4.8 5.1 4.1 4.0

D1 (24h) 4.7 <3 <3 2.3 4.0

34 J7 <1 <1 <1 <2 <2

J14 <1 <1 <1 <1 <1

D28 <1 <1 <1 <1 <1

OJ 5.1 4.8 5.1 4.1 4.0

D1 (24h) <3 <3 <3 <2 3.6

J7 <1 <1 <1 <1 <1

J14 <1 <1 <1 <1 <1

D28 <1 <1 <1 <1 <1

OJ 5.1 4.8 5.1 4.1 4.0

JI1 (24h) 5.1 _ <3 <3 3.9 4.1

38 J7 <1 <1 <1 <2 <2

J14 <1 <1 <1 <1 1

J28 <I <1 <I <1 <1

J0 5.1 5.1 5.7 4.3 4.0

D 1 (24h) 4.9 3.0 4.1 4.7 3.5

62 J7 _ 2.9 <1 <1 _ _> 4.5 3.3

J14 <1 _ <<1 5.0 3.0

_______ 1J28 <1 <1 <1 5.0 2.0

OJ 5.2 5.1 5.7 4.1 3.8

63 Jl (24h) 4.8 2.0 3.6 4.5 3.5

J7 <1 <1 <1> 4.5 4.1

D14 <1 <1> 3.5 5.9 4.3

D28 <1 <1> 3.5 5.7 2.0

  CD - \ q oo oD Analysis of the results shows that samples 34, 35, and 38 containing 2-methyl 1,3-propanediol (MPDiol glycol) and EDTA respond

  to the standards of the French Pharmacopoeia for all of the germs tested.

  However, samples 62 and 63 not containing EDTA but containing MPDiol glycol do not meet the standards of the

French Pharmacopoeia.

Claims (11)

  1. Aqueous cosmetic composition without preservative containing: - from 0.5 to 15% by weight of a hydrating agent chosen from diols in C3-C4 alone or as a mixture, and   - from 0.01 to 0.5% by weight of a sequestering agent.   2. Cosmetic composition according to claim 1 containing   from 0.5 to 5%, preferably from 3 to 4.5%, by weight of said hydrating agent.   3. Cosmetic composition according to one of claims   above, wherein said hydrating agent is 2-methyl 1,3-   propanediol, alone or in admixture with another C3-C4 diol.   4. Cosmetic composition according to any one of   preceding claims, containing 0.1 to 0.4% by weight of said agent   sequestrant. 5. Cosmetic composition according to. any of   the preceding claims, wherein said sequestering agent is a salt   EDTA, preferably tetrasodium EDTA.   6. Cosmetic composition according to any one of   previous claims, further containing an emulsifier.   7. Cosmetic composition according to claim 6 containing from 0.1 to 5% by weight, preferably from 0.3 to 1% by weight of said emulsifying agent. 8. Cosmetic composition according to any one of   claims 6 or 7 wherein said emulsifying agent is a derivative   fatty acid containing from 6 to 14 carbon atoms.   9. Cosmetic composition according to claim 8 in   which said fatty acid derivative is sodium laurate.   10. Use of a combination of a hydrating agent chosen from C3-C4 diols and a sequestering agent in an effective amount, in an aqueous cosmetic composition without preservative, to prevent   development of microorganisms in said cosmetic composition.   11. Use according to claim 10, said aqueous cosmetic composition without preservative containing: - from 0.5 to 15% by weight of a moisturizing agent chosen from C3-C4 diols alone or as a mixture, and   - from 0.01 to 0.5% by weight of a sequestering agent.