FR2661833A1 - Method for preparing a water-soluble extract of fenugreek seeds and product obtained - Google Patents

Abstract

The invention relates to a method for preparing an extract of fenugreek seeds and to the product obtained. The method of the invention consists in inducing the swelling of fenugreek, preferably of Indian origin, by pouring filtered water at 45 DEG C onto it in the proportion of 100 ml per 100 g of seeds, then in adding twice the amount of water 15 minutes later and in leaving the preparation to infuse while stirring slowly for 45 minutes, the resulting solution thereafter being filtered and the seeds then removed by draining or centrifugation, after which the solution is subjected to a diafiltration through a calibrated porous membrane possessing a threshold of retention of substances whose molecular weight is equal to or above 300,000. The product according to the invention is intended for incorporation in cosmetological and, where appropriate, pharmaceutical compositions, in particular in preparations that retard dermal ageing or that nourish the dermis, for example those intended for use after exposure to the sun, or alternatively in specialities used for the treatment of wounds and burns.

Description

PROCESS FOR THE PREPARATION OF WATER-SOLUBLE SEED EXTRACT
OF FENUGREC AND PRODUCT OBTAINED
The subject of the present invention is a process for preparing fenugreek seed extract and the product obtained.

The product according to the invention is intended to be incorporated into cosmetic and possibly pharmaceutical compositions, in particular in preparations slowing down dermal aging, or nourishing the dermis, for example intended for use after exposure to the sun, or else in specialties used for the treatment of wounds and burns.

Many preparations of plant origin based on fenugreek have been described in the literature since ancient times.

For example, in the last book of the SMITH papyrus, 3000 years before Jesus Christ, we quote the Hemalt. This interpretation corresponds to the applications of the trigonelle described by PLINE, then by the Arabs. The Ebers papyrus, 2000 years before Jesus
Christ mentions it in the treatment of burns. However, the known preparations have a very limited trophic power and are most often devoid of regenerative and healing properties.

 The water-soluble extract of fenugreek seeds obtained by the process which is the subject of the present invention, on the contrary, exhibits remarkable trophic, regenerative and healing properties, as well as very good tolerance by the dermis.

The process according to the invention consists in causing the swelling of fenugreek, preferably originating in India, by pouring filtered water at 450C at the rate of 100 ml per 100 gr of seeds, then adding 15 minutes later double the amount of water and leave to infuse while stirring slowly for 45 minutes, the resulting solution then being filtered and the seeds removed by spin or centrifugation, after which the solution is subjected to a diafiltration on a calibrated porous membrane having a retention threshold of substances with a molecular weight of 300,000 or more.

 Other characteristics will emerge from the following detailed description, which relates to a nonlimiting example of one of the embodiments.

The product according to the present invention is obtained by applying the process below
We start by obtaining the maximum swelling of fenugreek by pouring 100 ml of water at 45 "C previously filtered at 0.22 micron per 100 gr of seeds. After 15 minutes, 200 ml of water filtered at 0 is added, 22 micron at the same temperature and allowed to infuse while stirring slowly for 45 minutes.

About 140 ml of solution are obtained. This is filtered, then the swollen and moist seeds are removed by spin or centrifugation. The extract obtained is first of all bright yellow and browns slightly after a few hours.

The solution is then kept under stirring at 37-40 "C, then it is introduced into a diafiltration device equipped with porous membranes, the retention threshold of which is 300,000 and the apparent diameter of the pores approximately 2 angstroms. circulate the entire solution.

The diafiltered solution is recovered and then 0.25 g of chloramide T is introduced into it per 100 gr. The active extraction isolated after diafiltration is clear yellow.

The physicochemical characteristics of the extract are as follows
Ph: 6, Density: 1.002; Dry extract: 0.5%; Refractive index: 1.334.

The quantities, duration of treatment and molecular weight indicated above are given as an indication and can vary within significant proportions.

The active principle can be incorporated into excipients usually used in cosmetic and pharmaceutical compositions, aqueous vehicles or not, fatty substances of animal or vegetable origin, paraffinic derivatives, glycols, the various wetting agents, dispersants or emulsifiers, conservatives.

Thanks to its remarkable effectiveness, the product which has just been described lends itself particularly well to the preparation of cosmetic or pharmacological specialties by being used in a proportion of 10 to 30% in creams, ointments, lotions, drops or other solutions.

The positioning of the various constituent elements gives this product a maximum of useful effects which had not been obtained to date by similar products.

Claims (5)

CLAIMS 1 "Process for the preparation of water-soluble extract of fenugreek seeds, intended to be incorporated into cosmetic and possibly pharmaceutical compositions, in particular in preparations which slow down dermal aging, or nourish the dermis, for example intended to be used after exposure to sun, or in specialties used for the treatment of wounds and burns, characterized by a treatment consisting of first causing swelling of fenugreek, by pouring over warm filtered water, then adding a double amount of water also filtered and lukewarm, and to let infuse with slow stirring, the resulting solution then being filtered, then the seeds removed by wringing or centrifugation, after which the solution is subjected to a diafiltration on a calibrated porous membrane. 20 The method of claim 1, characterized in that the swelling of fenugreek is obtained by pouring over 100 ml of water at 450C previously filtered to 0.22 micron per 100 gr of seeds, 200 ml of water filtered to 0 , 22 micron at the same temperature being added approximately 15 minutes later, the mixture then being left to infuse while stirring slowly for 45 minutes, the solution obtained then being filtered and the seeds removed by spin or centrifugation, the resulting solution then being maintained with stirring at 37-4O0C, then passed in its entirety in a diafiltration apparatus equipped with porous membranes, the retention threshold of which is 300,000 and the apparent diameter of the pores approximately 2 angstroms, the operation being completed by introducing 0, 25 gr of chloramide T for 100 gr, in the final extract, it being specified that the quantities, treatment times and molecular weight indicated may vary around the values indicated. 30 A method according to any one of the preceding claims, characterized in that the fenugreek used originates from India. 40 Product characterized in that it is obtained by applying the process according to any one of the preceding claims. 50 Product according to claim 4, characterized in that its apparent molecular weight is less than 300,000, that it has a pH of 6, a density of 1.002, a refractive index of 1.334 and contains 0.5% of dry extract. 60 Cosmetic or pharmaceutical compositions characterized in that they contain the product according to any one of claims 4 and 5.