FR2660196A1 - Method for the manufacture of water-soluble cell extract of egg embryos and product obtained - Google Patents

Method for the manufacture of water-soluble cell extract of egg embryos and product obtained Download PDF

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Publication number
FR2660196A1
FR2660196A1 FR9004215A FR9004215A FR2660196A1 FR 2660196 A1 FR2660196 A1 FR 2660196A1 FR 9004215 A FR9004215 A FR 9004215A FR 9004215 A FR9004215 A FR 9004215A FR 2660196 A1 FR2660196 A1 FR 2660196A1
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powder
hours
filtered
suspension
product obtained
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Courmontagne Albert
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/982—Reproductive organs; Embryos, Eggs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Dermatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for the manufacture of water-soluble cell extract of egg embryos and to the product obtained. The method of the invention consists in treating pasteurised powdered egg yolk successively by means of several organic solvents such as acetone and ethanol, the crude product obtained being collected and brought into aqueous solution, the latter being subjected to a diafiltration through a calibrated porous membrane possessing a threshold of retention of substances whose molecular weight is equal to or below 100,000. The product obtained is intended for incorporation in pharmaceutical or cosmetological compositions, in particular in preparations for the treatment of burns and wounds, or preparations that retard dermal ageing or that nourish the dermis, for example those intended for use after exposure to the sun.

Description

PROCEDE DE FABRICATION D'EXTRAIT CELLULAIRE HYDROSOLUBLE
D'EMBRYONS D'OEUF ET PRODUIT OBTENU
La présente invention a pour objet un procédé de fabrication d'extrait cellulaire hydrosoluble d'embryons d'oeuf et le produit obtenu.PROCESS FOR MANUFACTURING WATER-SOLUBLE CELL EXTRACT
EGG EMBRYOS AND PRODUCT OBTAINED
The subject of the present invention is a process for manufacturing a water-soluble cellular extract from egg embryos and the product obtained.

Le produit obtenu est destiné à être incorporé à des compositions pharmaceutiques ou cosmétologiques notamment dans des préparations pour traitement des brûlures et plaies, ou des préparations ralentissant le vieillissement dermique, ou nourrissant le derme, par exemple destinées à être utilisées après exposition au soleil.The product obtained is intended to be incorporated into pharmaceutical or cosmetic compositions in particular in preparations for the treatment of burns and wounds, or preparations slowing down dermal aging, or nourishing the dermis, for example intended for use after exposure to the sun.

De nombreuses préparations cosmétologiques décrites dans les formulaires de cosmétologie contiennent de la lécithine comme émulsionnant et co-émulsionnant, de l'huile d'oeuf riche en acide oléique et linoléique, ou du jaune d'oeuf, introduit en particulier dans les shampoings et les crèmes, plus dans un but de marketing que scientifique. Aucune de ces préparations n'est à même de tirer parti des propriétés régénératrices et cicatrisantes de l'embryon d'oeuf.Many cosmetic preparations described in cosmetology forms contain lecithin as an emulsifier and co-emulsifier, egg oil rich in oleic and linoleic acid, or egg yolk, introduced in particular in shampoos and creams, more for marketing than scientific. None of these preparations is able to take advantage of the regenerative and healing properties of the egg embryo.

L'extrait cellulaire d'oeuf obtenu par le procédé suivant la présente invention est remarquable en ce qu 'il présente des propriétés trophiques, régénératrices et cicatrisantes, ainsi qu'une très bonne tolérance par le derme.The egg cell extract obtained by the process according to the present invention is remarkable in that it has trophic, regenerative and healing properties, as well as very good tolerance by the dermis.

Le procédé suivant l'invention consiste à traiter de la poudre pasteurisée de jaune d'oeuf successivement au moyen de plusieurs solvants organiques tels que acétone et éthanol, le produit brut obtenu étant recueilli, mis en solution aqueuse, celle-ci étant soumise à une diafiltration sur membrane poreuse calibrée présentant un seuil de rétention de substances dont le poids moléculaire est égal ou inférieur à 100 000. The process according to the invention consists in treating pasteurized egg yolk powder successively by means of several organic solvents such as acetone and ethanol, the crude product obtained being collected, put into aqueous solution, the latter being subjected to a diafiltration on a calibrated porous membrane having a retention threshold for substances whose molecular weight is equal to or less than 100,000.

D'autres caractéristiques ressortent de la description détaillée suivante, qui concerne un exemple non limitatif d'une des formes de réalisation.Other characteristics will emerge from the following detailed description, which relates to a nonlimiting example of one of the embodiments.

Le produit selon la présente invention est obtenu en appliquant le procédé ci-après
Tout d'abord, 100 grammes de poudre de jaune d'oeuf pasteurisé sont mis en suspension dans 300 ml d'acétone froid. Le mélange est agité vigoureusement pendant 3 heures à 3500 tours/minute, puis la suspension est filtrée sur verre fritté et l'on recueille une poudre jaune que l'on essore rapidement.The product according to the present invention is obtained by applying the process below
First, 100 grams of pasteurized egg yolk powder is suspended in 300 ml of cold acetone. The mixture is vigorously stirred for 3 hours at 3500 rpm, then the suspension is filtered through sintered glass and a yellow powder is collected which is wrung quickly.

La poudre obtenue est mise en suspension dans 300 ml d'éthanol froid et agitée vigoureusement pendant 3 heures à 1500 tours/ minute, puis la suspension est filtrée sur verre fritté et l'on recueille d'une part la solution éthanolique, et d'autre part une poudre qui est mise en suspension dans 150 ml d'acétone froid.The powder obtained is suspended in 300 ml of cold ethanol and vigorously stirred for 3 hours at 1500 revolutions / minute, then the suspension is filtered on sintered glass and the ethanolic solution is collected on the one hand, and on the other hand a powder which is suspended in 150 ml of cold acetone.

Le mélange est agité vigoureusement pendant 2 heures à 1500 tours/minute, puis la suspension est filtrée sur verre fritté et l'on recueille la solution acétonique, et une poudre qui est essorée puis sèchée à température ambiante sous vide pendant 48 heures.The mixture is vigorously stirred for 2 hours at 1500 revolutions / minute, then the suspension is filtered through sintered glass and the acetone solution is collected, and a powder which is wrung and then dried at room temperature under vacuum for 48 hours.

On obtient finalement 40 grammes d'une poudre blanc-jaunâtre, qui sont mis en solution dans de l'eau distillée et filtrée à 0,22 nìicron et la solution est maintenue sous agitation à 250C pendant 4 heures puis centrifugée à 9000 g. La solution est récupérée et passée en totalité dans un appareil à diafiltrer équipé de membranes poreuses dont le seuil de rétention est de 100 000. La solution diafiltrée est récupérée puis l'on introduit 0,05 gramme de conservateur bactéricide pour 100 grammes de solution.40 grams of a yellowish-white powder are finally obtained, which are dissolved in distilled water and filtered at 0.22 nm and the solution is stirred at 250C for 4 hours and then centrifuged at 9000 g. The solution is recovered and passed entirely through a diafiltration device equipped with porous membranes, the retention threshold of which is 100,000. The diafiltered solution is recovered and then 0.05 grams of bactericidal preservative are introduced per 100 grams of solution.

Les quantités, durées de traitement et poids moléculaire indiqués ci-dessus sont donnés à titre indicatif et peuvent varier dans des proportions assez importantes.The quantities, duration of treatment and molecular weight indicated above are given as an indication and can vary within fairly large proportions.

Par ailleurs, les solutions acétonique et éthanolique sont mélangées puis distillées sous vide à 400C, ce qui permet d'obtenir 58 grammes d'huile d'oeuf.Furthermore, the acetone and ethanolic solutions are mixed and then distilled under vacuum at 400C, which allows to obtain 58 grams of egg oil.

L'extrait cellulaire hydrosoluble d'embryon d'oeuf est limpide, très légèrement jaune pale. Il a un ph de 6, une densité de 1,002 et contient en particulier les acides aminés suivants
Alanine, Arginine, Acide aspartique, Cystine, Acide glutamique,
Glycine, Histidine, Isoleucine, Leucine, Lysine, Méthionine,
Phénylanine, Proline, Serine, Tréonine, Tryptophane, Tyrosine, et Valine. The water-soluble cell extract from the egg embryo is clear, very slightly pale yellow. It has a pH of 6, a density of 1.002 and contains in particular the following amino acids
Alanine, Arginine, Aspartic acid, Cystine, Glutamic acid,
Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine,
Phenylanine, Proline, Serine, Treonine, Tryptophan, Tyrosine, and Valine.

Le principe actif peut être incorporé à des excipients habituellement utilisés dans les compositions cosmétologiques et pharmaceutiques, les véhicules aqueux ou non, les corps gras d'origine animale ou végétale, les dérivés paraffiniques, les glycols, les divers agents mouillants, dispersants ou émulsifiants, les conservateurs.The active principle can be incorporated into excipients usually used in cosmetic and pharmaceutical compositions, aqueous or non-aqueous vehicles, fatty substances of animal or vegetable origin, paraffinic derivatives, glycols, various wetting agents, dispersants or emulsifiers, conservatives.

Grâce à sa remarquable efficacité, le produit qui vient d'être décrit se prête particulièrement bien à la confection de spécialités cosmétologiques ou pharmacologiques en étant utilisé dans une proportion de 10 à 30 % dans les solutions crèmes, pommades, lotions, gouttes ou autres.Thanks to its remarkable effectiveness, the product which has just been described lends itself particularly well to the preparation of cosmetological or pharmacological specialties by being used in a proportion of 10 to 30% in creams, ointments, lotions, drops or other solutions.

Le positionnement des divers éléments constitutifs donnent à ce produit un maximum d'effets utiles qui n'avaient pas été obtenus à ce jour par des produits similaires. The positioning of the various constituent elements gives this product a maximum of useful effects which had not been obtained to date by similar products.

Claims (6)

REVENDICATIONS 10 Procédé de fabrication d'extrait cellulaire hydrosoluble d'embryons d'oeuf destiné à être incorporé à des compositions pharmaceutiques ou cosmétologiques notamment dans des préparations pour traitement des brûlures et plaies, ou des préparations ralentissant le vieillissement dermique, ou nourrissant le derme, caractérisé par le traitement de poudre pasteurisée de jaune d'oeuf au moyen d'un ou plusieurs solvants organiques tels que acétone et éthanol, le produit brut obtenu étant recueilli, mis en solution aqueuse, celle-ci étant centrifugée puis soumise à une diafiltration sur membrane poreuse, un conservateur bactéricide étant finalement ajouté au produit obtenu.10 Process for the production of water-soluble cellular extract from egg embryos intended for incorporation into pharmaceutical or cosmetic compositions, in particular in preparations for the treatment of burns and wounds, or preparations which slow dermal aging, or nourish the dermis, characterized by the treatment of pasteurized egg yolk powder using one or more organic solvents such as acetone and ethanol, the crude product obtained being collected, put into aqueous solution, the latter being centrifuged and then subjected to a diafiltration on a membrane porous, a bactericidal preservative being finally added to the product obtained. 20 Procédé suivant la revendication 1, se caractérisant par le fait que la poudre de jaune d'oeuf pasteurisé est d'abord mise en suspension dans de l'acétone froid, le mélange agité vigoureusement pendant plusieurs heures, la suspension obtenue étant filtrée sur verre fritté pour recueillir une poudre que l'on essore rapidement, cette poudre étant ensuite mise en suspension dans de l'éthanol froid et le mélange agité vigoureusement pendant plusieurs heures, la suspension étant alors filtrée sur verre et la poudre à nouveau receuillie et mise en suspension dans de l'acétone froid, puis le mélange agité vigoureusement pendant 1 à 3 heures et filtré sur verre fritté pour obtenir une poudre qui est essorée puis séchée à température ambiante sous vide pendant au moins 24 heures pour donner finalement une poudre, qui est mise en solution dans de l'eau distillée, la solution étant alors maintenue sous agitation à environ 250C pendant plusieurs heures puis centrifugée et passée en totalité dans un appareil à diafiltrer équipé de membranes poreuses dont le seuil de rétention est inférieur ou égal 500 000.20 The method of claim 1, characterized in that the pasteurized egg yolk powder is first suspended in cold acetone, the mixture stirred vigorously for several hours, the suspension obtained being filtered through glass sintered to collect a powder which is wrung quickly, this powder then being suspended in cold ethanol and the mixture vigorously stirred for several hours, the suspension then being filtered through glass and the powder again collected and placed in suspension in cold acetone, then the mixture vigorously stirred for 1 to 3 hours and filtered on sintered glass to obtain a powder which is wrung out and then dried at room temperature under vacuum for at least 24 hours to finally give a powder, which is dissolved in distilled water, the solution then being stirred at around 250C for several hours and then centered fugitive and passed entirely through a diafiltration device equipped with porous membranes with a retention threshold of 500,000 or less. 30 Procédé suivant l'une quelconque des revendications précédentes, se caractérisant par le fait que 100 grammes de poudre de jaune d'oeuf pasteurisé sont mis en suspension dans 300 ml d'acétone froid, le mélange agité vigoureusement pendant 3 heures à 3500 lours/minute, la suspension obtenue étant filtrée sur verre fritté pour recueillir une poudre jaune que l'on essore rapidement, cette poudre étant mise en suspension dans 300 ml d'éthanol froid et le mélange agité vigoureusement pendant 3 heures à 1500 tours minute, la suspension étant alors filtrée sur verre fritté et la poudre à nouveau recueillie et mise en suspension dans 150 ml d'acétone froid, le mélange étant agité vigoureusement pendant 2 heures à 1500 tours/minute, puis la suspension filtrée sur verre fritté pour obtenir une poudre qui est essorée puis séchée à température ambiante sous vide pendant 48 heures pour donner finalement 40 -grammes d'une poudre blanc-jaunâtre, qui sont mis en solution dans de l'eau distillée filtrée à 0,22 micron, la solution étant alors maintenue sous agitation à 250C pendant 4 heures puis centrofugée à 9000 g, et passée en totalité dans un appareil à dia filtrer équipé de membranes poreuses dont le seuil de rétention est de 100 000, la solution diafiltrée étant finalement mélangée avec 0,05 gramme de conservateur bactéricide pour30 A method according to any one of the preceding claims, characterized in that 100 grams of pasteurized egg yolk powder are suspended in 300 ml of cold acetone, the mixture vigorously stirred for 3 hours at 3500 lours / minute, the suspension obtained being filtered through sintered glass to collect a yellow powder which is wrung quickly, this powder being suspended in 300 ml of cold ethanol and the mixture vigorously stirred for 3 hours at 1500 rpm, the suspension then being filtered on sintered glass and the powder collected again and suspended in 150 ml of cold acetone, the mixture being vigorously stirred for 2 hours at 1500 rpm, then the suspension filtered on sintered glass to obtain a powder which is wrung and then dried at room temperature under vacuum for 48 hours to finally give 40 -grams of a white-yellowish powder, which are put in solution in distilled water filtered to 0.22 micron, the solution then being kept under stirring at 250C for 4 hours then centrifuged at 9000 g, and passed completely through a filtering device equipped with porous membranes, the threshold of retention is 100,000, the diafiltered solution being finally mixed with 0.05 gram of bactericidal preservative to 100 grammes de solution, étant précisé que les quantités, durées de traitement et poids moléculaire indiqués peuvent varier autour des valeurs indiquées. 100 grams of solution, it being specified that the quantities, duration of treatment and molecular weight indicated may vary around the values indicated. 40 Procédé suivant l'une quelconque des revendications précédentes, se caractérisant par le fait que les solutions obtenues par filtrage après les traitements par solvants organiques sont recueillies, mélangées puis distillées sous vide, pour obtenir de l'huile d'oeuf. 40 Method according to any one of the preceding claims, characterized in that the solutions obtained by filtration after the treatments with organic solvents are collected, mixed and then distilled under vacuum, to obtain egg oil. 50 Produit caractérisé par le fait qu'il est obtenu par l'appli- cation du procédé selon l'une quelconque des revendications précédentes.50 Product characterized in that it is obtained by the application of the process according to any one of the preceding claims. 60 Produit suivant la reveneication 5, se caractérisant par le fait que son poids moléculaire apparent est inférieur à 100 000, qu'il à un ph de 6, une densité de 1,002 et contient en particulier les acides aminés suivants : Alanine, Arginine, Acide aspartique, Cystine, Acide glutamique, Glycine, Histidine,60 Product according to reveneication 5, characterized in that its apparent molecular weight is less than 100,000, that it has a pH of 6, a density of 1.002 and contains in particular the following amino acids: Alanine, Arginine, Acid aspartic, Cystine, Glutamic acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Méthionine, Phénylanine, Proline,Isoleucine, Leucine, Lysine, Methionine, Phenylanine, Proline, Serine, Tréonine, Tryptophane, Tyrosine et Valine. Serine, Tréonine, Tryptophane, Tyrosine and Valine. 7" Compositions cosmétologiques ou pharmaceutiques se caractérisant par le fait qu'ils contiennent le produit suivant l'une quelconque des revendications 5 et 6.  7 "Cosmetic or pharmaceutical compositions characterized by the fact that they contain the product according to any one of claims 5 and 6.
FR9004215A 1990-03-28 1990-03-28 Method for the manufacture of water-soluble cell extract of egg embryos and product obtained Pending FR2660196A1 (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3558256A4 (en) * 2016-12-21 2019-12-11 Unilever PLC Personal care compositions with glutathione precursor comprising nicotinamide and amino acids
EP3558246A4 (en) * 2016-12-21 2020-01-08 Unilever PLC Personal care compositions with glutathione precursor comprising 4-substituted resorcinols and amino acids
US10751267B2 (en) 2016-12-21 2020-08-25 Conopco, Inc. Personal care compositions comprising poorly soluble compounds
US11077039B2 (en) 2016-12-21 2021-08-03 Conopco, Inc. Topical skin lightening additive and composition with amino acids and nicotinamide compounds
US11337908B2 (en) 2016-12-21 2022-05-24 Conopco, Inc. Personal care compositions with cystine
US11540984B2 (en) 2018-05-23 2023-01-03 Conopco, Inc. Nanoemulsions and a method for making the same

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3558256A4 (en) * 2016-12-21 2019-12-11 Unilever PLC Personal care compositions with glutathione precursor comprising nicotinamide and amino acids
EP3558246A4 (en) * 2016-12-21 2020-01-08 Unilever PLC Personal care compositions with glutathione precursor comprising 4-substituted resorcinols and amino acids
US10751267B2 (en) 2016-12-21 2020-08-25 Conopco, Inc. Personal care compositions comprising poorly soluble compounds
US10980718B2 (en) 2016-12-21 2021-04-20 Conopco, Inc. Personal care compositions comprising poorly soluble compounds
US11077039B2 (en) 2016-12-21 2021-08-03 Conopco, Inc. Topical skin lightening additive and composition with amino acids and nicotinamide compounds
US11260005B2 (en) 2016-12-21 2022-03-01 Conopco, Inc. Personal care compositions with glutathione precursor comprising 4-substituted resorcinols and amino acids
US11337908B2 (en) 2016-12-21 2022-05-24 Conopco, Inc. Personal care compositions with cystine
US11596586B2 (en) 2016-12-21 2023-03-07 Conopco, Inc. Personal care compositions with glutathione precursor comprising 4-substituted resorcinols and amino acids
US11666519B2 (en) 2016-12-21 2023-06-06 Conopco, Inc. Personal care compositions with cystine
US11759412B2 (en) 2016-12-21 2023-09-19 Conopco, Inc. Personal care compositions with glutathione precursor comprising 4-substituted resorcinols and amino acids
US11540984B2 (en) 2018-05-23 2023-01-03 Conopco, Inc. Nanoemulsions and a method for making the same

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