FR2489364A1 - Nouveaux plasmides hybrides et microorganismes les comportant - Google Patents
Nouveaux plasmides hybrides et microorganismes les comportant Download PDFInfo
- Publication number
- FR2489364A1 FR2489364A1 FR8018755A FR8018755A FR2489364A1 FR 2489364 A1 FR2489364 A1 FR 2489364A1 FR 8018755 A FR8018755 A FR 8018755A FR 8018755 A FR8018755 A FR 8018755A FR 2489364 A1 FR2489364 A1 FR 2489364A1
- Authority
- FR
- France
- Prior art keywords
- plasmid
- dna
- yeast
- hind iii
- eco
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 85
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 37
- 244000005700 microbiome Species 0.000 title claims description 8
- 108020000946 Bacterial DNA Proteins 0.000 title claims description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title description 24
- 229910052757 nitrogen Inorganic materials 0.000 title description 12
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 claims abstract description 41
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 108020004414 DNA Proteins 0.000 claims description 48
- 101150050575 URA3 gene Proteins 0.000 claims description 5
- 241000701959 Escherichia virus Lambda Species 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 32
- 241000588724 Escherichia coli Species 0.000 description 11
- 229960000723 ampicillin Drugs 0.000 description 10
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 239000013598 vector Substances 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 101150090724 3 gene Proteins 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 3
- 102000004533 Endonucleases Human genes 0.000 description 3
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 3
- 244000007853 Sarothamnus scoparius Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241000702208 Shigella phage SfX Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 101150085823 hsdR gene Proteins 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 108091092742 A-DNA Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101100238763 Bacillus subtilis hsdRM gene Proteins 0.000 description 1
- 241000272194 Ciconiiformes Species 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
- 241000701533 Escherichia virus T4 Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101150090128 PCM1 gene Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241001664469 Tibicina haematodes Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- JJWKPURADFRFRB-UHFFFAOYSA-N carbonyl sulfide Chemical compound O=C=S JJWKPURADFRFRB-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 101150102883 hsdM gene Proteins 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- RGCLLPNLLBQHPF-HJWRWDBZSA-N phosphamidon Chemical compound CCN(CC)C(=O)C(\Cl)=C(/C)OP(=O)(OC)OC RGCLLPNLLBQHPF-HJWRWDBZSA-N 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 101150116440 pyrF gene Proteins 0.000 description 1
- 101150007503 rps1 gene Proteins 0.000 description 1
- 101150008822 rpsA gene Proteins 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8018755A FR2489364A1 (fr) | 1980-08-29 | 1980-08-29 | Nouveaux plasmides hybrides et microorganismes les comportant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8018755A FR2489364A1 (fr) | 1980-08-29 | 1980-08-29 | Nouveaux plasmides hybrides et microorganismes les comportant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2489364A1 true FR2489364A1 (fr) | 1982-03-05 |
| FR2489364B1 FR2489364B1 (enExample) | 1984-03-09 |
Family
ID=9245497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8018755A Granted FR2489364A1 (fr) | 1980-08-29 | 1980-08-29 | Nouveaux plasmides hybrides et microorganismes les comportant |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2489364A1 (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2568891A1 (fr) * | 1984-08-09 | 1986-02-14 | Transgene Sa | Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue |
| US4626505A (en) * | 1984-02-24 | 1986-12-02 | E. I. Du Pont De Nemours And Company | Selectable markers for yeast transformation |
| US4670399A (en) * | 1983-01-31 | 1987-06-02 | Stauffer Chemical Co. | Hybrid plasmid with marker |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2441659A1 (fr) * | 1978-11-14 | 1980-06-13 | Anvar | Nouveaux plasmides hybrides et microorganismes les contenant |
-
1980
- 1980-08-29 FR FR8018755A patent/FR2489364A1/fr active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2441659A1 (fr) * | 1978-11-14 | 1980-06-13 | Anvar | Nouveaux plasmides hybrides et microorganismes les contenant |
Non-Patent Citations (1)
| Title |
|---|
| EXBK/79 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4670399A (en) * | 1983-01-31 | 1987-06-02 | Stauffer Chemical Co. | Hybrid plasmid with marker |
| US4626505A (en) * | 1984-02-24 | 1986-12-02 | E. I. Du Pont De Nemours And Company | Selectable markers for yeast transformation |
| FR2568891A1 (fr) * | 1984-08-09 | 1986-02-14 | Transgene Sa | Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2489364B1 (enExample) | 1984-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0367644B1 (fr) | Séquence de nucléotides codant pour une proteine à activité uréasique | |
| Mlouka et al. | The gas vesicle gene cluster from Microcystis aeruginosa and DNA rearrangements that lead to loss of cell buoyancy | |
| US4359535A (en) | Autonomously replicating DNA containing inserted DNA sequences | |
| FR2489363A1 (fr) | Nouveaux plasmides hybrides utiles notamment pour l'introduction des genes responsables de la fixation de l'azote dans des microorganismes et cellules eucaryotes; microorganismes et cellules eucaryotes contenant lesdits plasmides | |
| Delihas et al. | The SS ribosomai RNA of Euglena gracilis cytoplasmic ribosomes is closely homologous to the SS RNA of the trypanosomatid protozoa | |
| FR2500847A1 (fr) | Marqueurs genetiques selectifs pour cellules eucaryotes, procede de mise en oeuvre de tels marqueurs et application des cellules contenant un tel marqueur a la fabrication de proteines determinees apres leur transformation par un adn correspondant | |
| EP0295156B1 (fr) | Séquences de nucléotides codant pour des polypeptides dotés d'une activité larvicide vis-à-vis de lépidoptères | |
| FR2489364A1 (fr) | Nouveaux plasmides hybrides et microorganismes les comportant | |
| EP0258118B1 (fr) | Procédé de stabilisation d'un plasmide contenu dans une souche bactérienne et souche obtenue | |
| CA2341776C (fr) | Procede de transformation non-homologue de yarrowia lipolytica | |
| EP0643134B1 (fr) | Séquence d'acides nucléiques et plasmides comprenant au moins un mécanisme de résistance aux phages, bactéries les contenant et leur utilisation | |
| Gabant et al. | Isolation and location on the R27 map of two replicons and an incompatibility determinant specific for IncHI1 plasmids | |
| EP0262043B1 (fr) | Séquence d'ADN vecteurs, virus recombinants et procédé mettant en oeuvre des virus de la vaccine recombinants capables de se multiplier dans les cellules CHO | |
| Miller et al. | Plasmid transfer and expression in Rhodopseudomonas sphaeroides | |
| EP0452224A1 (fr) | Molécule d'ADN et plasmide comprenant au moins un mécanisme de résistance aux phages, bactéries les contenant et leur utilisation | |
| FR2780733A1 (fr) | Polynucleotide, vecteur et cellule hote le contenant, polypeptide correspondant, procede pour leur production et leur utilisation | |
| FR2677996A1 (fr) | Vecteurs de clonage et/ou d'expression preparation et utilisation. | |
| CA2091061C (fr) | Fragments d'adn codant pour un mecanisme resistance aux bacteriophages | |
| CA1301678C (en) | Promoter, vector having the improved promoter, plasmid having the vector, and transformer with the plasmid | |
| EP0528925B1 (fr) | Nouveaux vecteurs de clonage et/ou d'expression, procede de preparation et leur utilisation | |
| FR2462476A1 (fr) | Vecteur cosmide possedant un gene de resistance a la kanamycine | |
| EP0771872A1 (fr) | Procédé de polymèrisation de séquences d'acides nucléiques et ses applications | |
| FR2753202A1 (fr) | Bacteries multiresistantes, procedes d'obtention et utilisation | |
| EP1056872B1 (fr) | Sequences d'adn contenant un mecanisme de transfert par conjugaison | |
| EP0764724A1 (fr) | Séquences d'acides nucléiques et plasmides comprenant au moins un mécanisme de résistance aux phages, bactéries les contenant et leur utilisation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |