FR2477176A1 - Procede de stabilisation du produit de reaction decelable forme dans les dosages immologiques d'enzymes - Google Patents
Procede de stabilisation du produit de reaction decelable forme dans les dosages immologiques d'enzymes Download PDFInfo
- Publication number
- FR2477176A1 FR2477176A1 FR8104239A FR8104239A FR2477176A1 FR 2477176 A1 FR2477176 A1 FR 2477176A1 FR 8104239 A FR8104239 A FR 8104239A FR 8104239 A FR8104239 A FR 8104239A FR 2477176 A1 FR2477176 A1 FR 2477176A1
- Authority
- FR
- France
- Prior art keywords
- reaction
- enzyme
- reaction product
- peroxidase
- stabilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract description 13
- 239000007795 chemical reaction product Substances 0.000 title abstract description 10
- 230000000087 stabilizing effect Effects 0.000 title description 3
- 238000001952 enzyme assay Methods 0.000 title description 2
- 239000011541 reaction mixture Substances 0.000 abstract description 13
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract description 12
- 239000000758 substrate Substances 0.000 abstract description 11
- 150000003839 salts Chemical class 0.000 abstract description 9
- 230000006641 stabilisation Effects 0.000 abstract description 6
- 238000011105 stabilization Methods 0.000 abstract description 6
- 239000003381 stabilizer Substances 0.000 abstract description 4
- 150000004764 thiosulfuric acid derivatives Chemical class 0.000 abstract description 4
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 16
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 12
- 102000013415 peroxidase activity proteins Human genes 0.000 description 11
- 239000000047 product Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 7
- 239000003638 chemical reducing agent Substances 0.000 description 7
- 239000011734 sodium Substances 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 4
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 4
- 235000010262 sodium metabisulphite Nutrition 0.000 description 4
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 3
- -1 H 2 SO 4 Proteins 0.000 description 3
- 150000004985 diamines Chemical class 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 239000012429 reaction media Substances 0.000 description 3
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 3
- 235000019345 sodium thiosulphate Nutrition 0.000 description 3
- 101710132601 Capsid protein Proteins 0.000 description 2
- 229940126062 Compound A Drugs 0.000 description 2
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 2
- 239000004296 sodium metabisulphite Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
- KWVPRPSXBZNOHS-UHFFFAOYSA-N 2,4,6-Trimethylaniline Chemical compound CC1=CC(C)=C(N)C(C)=C1 KWVPRPSXBZNOHS-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- SNCJAJRILVFXAE-UHFFFAOYSA-N 9h-fluorene-2,7-diamine Chemical compound NC1=CC=C2C3=CC=C(N)C=C3CC2=C1 SNCJAJRILVFXAE-UHFFFAOYSA-N 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108060006006 Cytochrome-c peroxidase Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010023244 Lactoperoxidase Proteins 0.000 description 1
- 102000045576 Lactoperoxidases Human genes 0.000 description 1
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 1
- AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 description 1
- 229910004879 Na2S2O5 Inorganic materials 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 229940057428 lactoperoxidase Drugs 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- PTLRDCMBXHILCL-UHFFFAOYSA-M sodium arsenite Chemical compound [Na+].[O-][As]=O PTLRDCMBXHILCL-UHFFFAOYSA-M 0.000 description 1
- 229940001584 sodium metabisulfite Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-L thiosulfate(2-) Chemical compound [O-]S([S-])(=O)=O DHCDFWKWKRSZHF-UHFFFAOYSA-L 0.000 description 1
- 238000000954 titration curve Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12681780A | 1980-03-03 | 1980-03-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2477176A1 true FR2477176A1 (fr) | 1981-09-04 |
| FR2477176B1 FR2477176B1 (enrdf_load_html_response) | 1984-09-14 |
Family
ID=22426850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8104239A Granted FR2477176A1 (fr) | 1980-03-03 | 1981-03-03 | Procede de stabilisation du produit de reaction decelable forme dans les dosages immologiques d'enzymes |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS56137155A (enrdf_load_html_response) |
| BE (1) | BE887771A (enrdf_load_html_response) |
| DE (1) | DE3107904A1 (enrdf_load_html_response) |
| FR (1) | FR2477176A1 (enrdf_load_html_response) |
| GB (1) | GB2070767B (enrdf_load_html_response) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4842997A (en) * | 1982-03-03 | 1989-06-27 | National Research Development Corporation | Enhanced luminescent and luminometric assay |
| JPS63258329A (ja) * | 1987-04-13 | 1988-10-25 | Taitoo:Kk | カ−ド発行装置 |
| JPH01135573U (enrdf_load_html_response) * | 1988-03-10 | 1989-09-18 | ||
| JPH0358761U (enrdf_load_html_response) * | 1989-10-16 | 1991-06-07 | ||
| US5225325A (en) * | 1990-03-02 | 1993-07-06 | Ventana Medical Systems, Inc. | Immunohistochemical staining method and reagents therefor |
| GB9723773D0 (en) * | 1997-11-12 | 1998-01-07 | Univ Glasgow | Haptoglobin assay |
| GB9924180D0 (en) * | 1999-10-12 | 1999-12-15 | Univ Glasgow | Assays for mastitis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6608106A (enrdf_load_html_response) * | 1966-06-10 | 1967-12-11 | ||
| US4169012A (en) * | 1976-09-24 | 1979-09-25 | Akzona Incorporated | Stabilized peroxidase reagent for enzyme immunoassay |
| US4234680A (en) * | 1979-08-03 | 1980-11-18 | Calbiochem-Behring Corp. | Method for terminating a peroxidase catalyzed reaction |
| US4252896A (en) * | 1980-01-07 | 1981-02-24 | Abbott Laboratories | Method of stabilizing peroxidase in a serum protein based medium |
-
1981
- 1981-02-02 GB GB8103120A patent/GB2070767B/en not_active Expired
- 1981-02-26 JP JP2620781A patent/JPS56137155A/ja active Granted
- 1981-03-02 DE DE19813107904 patent/DE3107904A1/de not_active Ceased
- 1981-03-03 FR FR8104239A patent/FR2477176A1/fr active Granted
- 1981-03-03 BE BE0/203993A patent/BE887771A/fr not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6608106A (enrdf_load_html_response) * | 1966-06-10 | 1967-12-11 | ||
| US4169012A (en) * | 1976-09-24 | 1979-09-25 | Akzona Incorporated | Stabilized peroxidase reagent for enzyme immunoassay |
| US4234680A (en) * | 1979-08-03 | 1980-11-18 | Calbiochem-Behring Corp. | Method for terminating a peroxidase catalyzed reaction |
| US4252896A (en) * | 1980-01-07 | 1981-02-24 | Abbott Laboratories | Method of stabilizing peroxidase in a serum protein based medium |
Also Published As
| Publication number | Publication date |
|---|---|
| BE887771A (fr) | 1981-09-03 |
| GB2070767A (en) | 1981-09-09 |
| FR2477176B1 (enrdf_load_html_response) | 1984-09-14 |
| JPS56137155A (en) | 1981-10-26 |
| GB2070767B (en) | 1983-07-27 |
| JPS629862B2 (enrdf_load_html_response) | 1987-03-03 |
| DE3107904A1 (de) | 1982-01-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |