FR2471410A2 - Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement - Google Patents
Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement Download PDFInfo
- Publication number
- FR2471410A2 FR2471410A2 FR7930820A FR7930820A FR2471410A2 FR 2471410 A2 FR2471410 A2 FR 2471410A2 FR 7930820 A FR7930820 A FR 7930820A FR 7930820 A FR7930820 A FR 7930820A FR 2471410 A2 FR2471410 A2 FR 2471410A2
- Authority
- FR
- France
- Prior art keywords
- determn
- electrode
- protease
- assay
- carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 title claims abstract description 9
- 239000004365 Protease Substances 0.000 title claims abstract description 9
- 239000000137 peptide hydrolase inhibitor Substances 0.000 title claims abstract description 9
- 239000000758 substrate Substances 0.000 title abstract description 5
- 150000001412 amines Chemical class 0.000 title abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 150000001408 amides Chemical class 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 13
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 238000003556 assay Methods 0.000 claims description 9
- 102000035195 Peptidases Human genes 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 4
- 239000005662 Paraffin oil Substances 0.000 claims description 3
- 239000004809 Teflon Substances 0.000 claims description 3
- 229920006362 Teflon® Polymers 0.000 claims description 3
- 239000012736 aqueous medium Substances 0.000 claims description 3
- 238000006911 enzymatic reaction Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 claims 2
- XVHWVDFGKDNOBO-AWEZNQCLSA-N (2s)-2-amino-5-(diaminomethylideneamino)-n-(4-methoxynaphthalen-2-yl)pentanamide Chemical compound C1=CC=C2C(OC)=CC(NC(=O)[C@@H](N)CCCNC(N)=N)=CC2=C1 XVHWVDFGKDNOBO-AWEZNQCLSA-N 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 239000004475 Arginine Substances 0.000 abstract 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 abstract 1
- 238000004082 amperometric method Methods 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 abstract 1
- 230000023555 blood coagulation Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 125000004433 nitrogen atom Chemical group N* 0.000 abstract 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108090000190 Thrombin Proteins 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- PKTSCJXWLVREKX-UHFFFAOYSA-N n-butyl-n-methylnitrous amide Chemical compound CCCCN(C)N=O PKTSCJXWLVREKX-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229960004072 thrombin Drugs 0.000 description 2
- QDAKPTLLVBBHMP-UHFFFAOYSA-N 2-n-phenylbenzene-1,2-diamine;hydrochloride Chemical compound Cl.NC1=CC=CC=C1NC1=CC=CC=C1 QDAKPTLLVBBHMP-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000012911 assay medium Substances 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
- -1 chloride methoxy-2-naphthylamide Chemical compound 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000004154 complement system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000004832 voltammetry Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Neurosurgery (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7930820A FR2471410A2 (fr) | 1979-12-17 | 1979-12-17 | Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement |
| DE8080102107T DE3061860D1 (en) | 1979-04-24 | 1980-04-18 | Process for the determination of proteases and antiproteases |
| EP80102107A EP0018002B1 (de) | 1979-04-24 | 1980-04-18 | Neues Bestimmungsverfahren für Proteasen und Antiproteasen |
| US06/142,655 US4304853A (en) | 1979-04-24 | 1980-04-22 | Method of determination for proteases and antiproteases |
| CA000350447A CA1149019A (en) | 1979-04-24 | 1980-04-23 | Method of determination for proteases and antiproteases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7930820A FR2471410A2 (fr) | 1979-12-17 | 1979-12-17 | Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2471410A2 true FR2471410A2 (fr) | 1981-06-19 |
| FR2471410B2 FR2471410B2 (enExample) | 1984-02-17 |
Family
ID=9232833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7930820A Granted FR2471410A2 (fr) | 1979-04-24 | 1979-12-17 | Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2471410A2 (enExample) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2317280A1 (fr) * | 1975-07-11 | 1977-02-04 | Kabi Ab | Nouveaux substrats chromogenes pour la thrombine |
-
1979
- 1979-12-17 FR FR7930820A patent/FR2471410A2/fr active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2317280A1 (fr) * | 1975-07-11 | 1977-02-04 | Kabi Ab | Nouveaux substrats chromogenes pour la thrombine |
Non-Patent Citations (2)
| Title |
|---|
| CA1974 * |
| EXBK/73 * |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2471410B2 (enExample) | 1984-02-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CL | Concession to grant licences |