FR2444712A1 - Prodn. of nutrient substrates contg. low methoxy pectin - which is gelled with polyvalent metal cation - Google Patents

Prodn. of nutrient substrates contg. low methoxy pectin - which is gelled with polyvalent metal cation

Info

Publication number
FR2444712A1
FR2444712A1 FR7931004A FR7931004A FR2444712A1 FR 2444712 A1 FR2444712 A1 FR 2444712A1 FR 7931004 A FR7931004 A FR 7931004A FR 7931004 A FR7931004 A FR 7931004A FR 2444712 A1 FR2444712 A1 FR 2444712A1
Authority
FR
France
Prior art keywords
pectin
esp
pref
prodn
gelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7931004A
Other languages
French (fr)
Other versions
FR2444712B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Conviron Inc
Original Assignee
Conviron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/970,347 external-priority patent/US4241186A/en
Priority claimed from US06/006,253 external-priority patent/US4282317A/en
Application filed by Conviron Inc filed Critical Conviron Inc
Publication of FR2444712A1 publication Critical patent/FR2444712A1/en
Application granted granted Critical
Publication of FR2444712B1 publication Critical patent/FR2444712B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Prodn. of biological nutrients substrates comprises combining predetermined amts. of (a) liq. nutrient; (b) pectin material (I) of low (pref. about 5%) methoxy content and (c) a growth-permitting gel which contains among other things polyvalent metal cations which gel (I). Pref. (a) and (b) are mixed first to provide 10-50g (I) per 1, and the total vol. of this mixt. is 3-20 times that of (c). Esp. a mixt. of (a) and (b) is added to a culture vessel (Petri dish) which contains a first layer of (c). The medium is pref. buffered with Na2HPO4, NaH2PO4, Na3PO4, NaHCO3, or Na2CO3 and the metal for gelling (I) is esp. Ca2+ used at 100-125 mg per g (I). The substrates can be packaged and sterilised ready for use so are esp. suitable for teaching laboratories etc. which have limited resources for making their own media. The mixts. can be used hot, warm or cooled (contrast agar) and are suitable for prokaryotic and eukaryotic organisms, cells and tissues. Pectin is cheaper than agar and only a few microorganisms are capable of hydrolysing gelled pectin.
FR7931004A 1978-12-18 1979-12-18 Prodn. of nutrient substrates contg. low methoxy pectin - which is gelled with polyvalent metal cation Granted FR2444712A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US05/970,347 US4241186A (en) 1978-12-18 1978-12-18 Pectin culture media and method
US325879A 1979-01-15 1979-01-15
US06/006,253 US4282317A (en) 1979-01-15 1979-02-21 Pectin culture media and method

Publications (2)

Publication Number Publication Date
FR2444712A1 true FR2444712A1 (en) 1980-07-18
FR2444712B1 FR2444712B1 (en) 1984-02-24

Family

ID=27357359

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7931004A Granted FR2444712A1 (en) 1978-12-18 1979-12-18 Prodn. of nutrient substrates contg. low methoxy pectin - which is gelled with polyvalent metal cation

Country Status (2)

Country Link
DE (1) DE2950776A1 (en)
FR (1) FR2444712A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2970948A (en) * 1953-10-22 1961-02-07 Sunkist Growers Inc Method of preparing dry culture media
US3352761A (en) * 1964-06-12 1967-11-14 S B Penick & Company Fermentation media for industrial fermentations comprising amylopectin as essentially the sole carbohydrate source
FR2182074A1 (en) * 1972-04-26 1973-12-07 Miles Lab

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2970948A (en) * 1953-10-22 1961-02-07 Sunkist Growers Inc Method of preparing dry culture media
US3352761A (en) * 1964-06-12 1967-11-14 S B Penick & Company Fermentation media for industrial fermentations comprising amylopectin as essentially the sole carbohydrate source
FR2182074A1 (en) * 1972-04-26 1973-12-07 Miles Lab

Also Published As

Publication number Publication date
DE2950776C2 (en) 1988-01-21
FR2444712B1 (en) 1984-02-24
DE2950776A1 (en) 1980-07-03

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