FR2432171A1 - METHOD AND KIT FOR USING SEMI-PERMEABLE MICROCAPSULES FOR THE DETERMINATION OF A BIOLOGICALLY ACTIVE AND FREE SUBSTANCE - Google Patents

METHOD AND KIT FOR USING SEMI-PERMEABLE MICROCAPSULES FOR THE DETERMINATION OF A BIOLOGICALLY ACTIVE AND FREE SUBSTANCE

Info

Publication number
FR2432171A1
FR2432171A1 FR7915709A FR7915709A FR2432171A1 FR 2432171 A1 FR2432171 A1 FR 2432171A1 FR 7915709 A FR7915709 A FR 7915709A FR 7915709 A FR7915709 A FR 7915709A FR 2432171 A1 FR2432171 A1 FR 2432171A1
Authority
FR
France
Prior art keywords
substance
antibody
determination
semi
free substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7915709A
Other languages
French (fr)
Other versions
FR2432171B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Damon Corp
Original Assignee
Damon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/963,932 external-priority patent/US4255411A/en
Application filed by Damon Corp filed Critical Damon Corp
Publication of FR2432171A1 publication Critical patent/FR2432171A1/en
Application granted granted Critical
Publication of FR2432171B1 publication Critical patent/FR2432171B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5089—Processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
    • F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
    • F02B75/00—Other engines
    • F02B75/02—Engines characterised by their cycles, e.g. six-stroke
    • F02B2075/022—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle
    • F02B2075/025—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle two

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Procédé et nécessaire pour déceler et doser une substance active dans un échantillon liquide contenant la subtance et des protéines. On fait incuber l'échantillon avec des microcapsules semi-perméables contenant de l'anticorps spécifique de la substance à déceler et un analogue de cette substance pouvant s'en distinguer. La membrane des capsules laisse passer la substance libre (qui entre en compétition avec l'analogue pour se fixer sur l'anticorps) mais retient les protéines naturelles et l'anticorps. On sépare ensuite l'anticorps et la substance libre. La détermination de la quantité d'analogue associé à l'anticorps ou présent dans le reste du système de réaction indique (par référence à une courbe témoin) la quantité de substance libre présente dans l'échantillon. Application : détermination d'une hormone (thyroxine, cortisol, testostérone ou digoxine).Method and necessary to detect and measure an active substance in a liquid sample containing the substance and proteins. The sample is incubated with semi-permeable microcapsules containing antibody specific for the substance to be detected and an analogue of this substance which can distinguish it. The membrane of the capsules allows the free substance to pass (which competes with the analog to bind to the antibody) but retains the natural proteins and the antibody. The antibody and the free substance are then separated. Determination of the amount of analog associated with the antibody or present in the rest of the reaction system indicates (by reference to a control curve) the amount of free substance present in the sample. Application: determination of a hormone (thyroxine, cortisol, testosterone or digoxin).

FR7915709A 1978-06-20 1979-06-19 METHOD AND KIT FOR USING SEMI-PERMEABLE MICROCAPSULES FOR THE DETERMINATION OF A BIOLOGICALLY ACTIVE AND FREE SUBSTANCE Granted FR2432171A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91736578A 1978-06-20 1978-06-20
US05/963,932 US4255411A (en) 1978-11-27 1978-11-27 Process of determining an immunogenic substance by competition with an antibody in a microcapsule

Publications (2)

Publication Number Publication Date
FR2432171A1 true FR2432171A1 (en) 1980-02-22
FR2432171B1 FR2432171B1 (en) 1985-02-15

Family

ID=27129721

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7915709A Granted FR2432171A1 (en) 1978-06-20 1979-06-19 METHOD AND KIT FOR USING SEMI-PERMEABLE MICROCAPSULES FOR THE DETERMINATION OF A BIOLOGICALLY ACTIVE AND FREE SUBSTANCE

Country Status (9)

Country Link
CA (1) CA1120397A (en)
CH (1) CH647869A5 (en)
DE (1) DE2924881A1 (en)
DK (1) DK256279A (en)
FR (1) FR2432171A1 (en)
GB (1) GB2023816B (en)
IT (1) IT1119920B (en)
NO (1) NO154409C (en)
SE (1) SE445680B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8006102L (en) * 1980-09-02 1982-03-03 Gambro Ab SET TO RECOVER A PEPTIDE-INHALING ASSOCIATION AND Means FOR IMPLEMENTATION OF THE SET
JPH0616046B2 (en) * 1982-03-19 1994-03-02 エキンズ，ロジャー フィリップ Free ligand assay method and kit
CA2105698A1 (en) * 1992-09-16 1994-03-17 Jozsef Hepp Label trapping assay-nonseparation binding assay method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3793445A (en) * 1970-04-29 1974-02-19 Wisconsin Alumni Res Found Reagent for radioimmunoassay
FR2228221A1 (en) * 1973-05-01 1974-11-29 Wisconsin Alumni Res Found
FR2288104A1 (en) * 1974-10-16 1976-05-14 Sjoholm Ingvar BIOLOGICALLY ACTIVE SUBSTANCE IMMOBILIZED IN A THREE-DIMENSIONAL POLYMER NETWORK AND ITS APPLICATIONS
GB1460631A (en) * 1973-03-19 1977-01-06 Summa Corp Method and apparatus for immunoassay with regeneration of immunadsorbent

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3793445A (en) * 1970-04-29 1974-02-19 Wisconsin Alumni Res Found Reagent for radioimmunoassay
GB1460631A (en) * 1973-03-19 1977-01-06 Summa Corp Method and apparatus for immunoassay with regeneration of immunadsorbent
FR2228221A1 (en) * 1973-05-01 1974-11-29 Wisconsin Alumni Res Found
US3970429A (en) * 1973-05-01 1976-07-20 Wisconsin Alumni Research Foundation Method for immunological determinations
FR2288104A1 (en) * 1974-10-16 1976-05-14 Sjoholm Ingvar BIOLOGICALLY ACTIVE SUBSTANCE IMMOBILIZED IN A THREE-DIMENSIONAL POLYMER NETWORK AND ITS APPLICATIONS

Also Published As

Publication number Publication date
CA1120397A (en) 1982-03-23
CH647869A5 (en) 1985-02-15
FR2432171B1 (en) 1985-02-15
DK256279A (en) 1980-01-28
SE445680B (en) 1986-07-07
NO154409C (en) 1986-09-10
GB2023816A (en) 1980-01-03
DE2924881A1 (en) 1980-01-17
IT7968303A0 (en) 1979-06-19
IT1119920B (en) 1986-03-19
NO154409B (en) 1986-06-02
NO792030L (en) 1979-12-21
SE7905101L (en) 1979-12-21
GB2023816B (en) 1983-02-23

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TP Transmission of property
ST Notification of lapse