FR2432170A1 - Procede de mesure de recepteurs d'antigene sur des membranes de cellules - Google Patents

Procede de mesure de recepteurs d'antigene sur des membranes de cellules

Info

Publication number
FR2432170A1
FR2432170A1 FR7914858A FR7914858A FR2432170A1 FR 2432170 A1 FR2432170 A1 FR 2432170A1 FR 7914858 A FR7914858 A FR 7914858A FR 7914858 A FR7914858 A FR 7914858A FR 2432170 A1 FR2432170 A1 FR 2432170A1
Authority
FR
France
Prior art keywords
cell membranes
antigen receptors
measuring antigen
degrees
antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7914858A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corning Glass Works
Original Assignee
Corning Glass Works
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Corning Glass Works filed Critical Corning Glass Works
Publication of FR2432170A1 publication Critical patent/FR2432170A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention se rapporte à l'analyse biologique. Elle concerne un procédé pour mesurer des récepteurs d'antigène sur des membranes de cellules, caractérisé en ce que les cellules sont mélangées avec une solution aqueuse isotonique d'un antigène immunoréactif soluble dans l'eau, marqué qui est d'une pureté d'au moins 95 % et est immunoréactif à au moins 40 %, le mélange est mis à incuber à une température comprise entre 4 degrés C et 40 degrés C pendant un temps suffisant pour qu'on arrive à la liaison à l'équilibre, on récolte les cellules à partir du mélange ayant subi l'incubation, et on mesure la quantité d'antigène marqué fixée sur les cellules récoltées. Utilisation par les laboratoires d'analyse.
FR7914858A 1978-06-12 1979-06-11 Procede de mesure de recepteurs d'antigene sur des membranes de cellules Withdrawn FR2432170A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US91434778A 1978-06-12 1978-06-12

Publications (1)

Publication Number Publication Date
FR2432170A1 true FR2432170A1 (fr) 1980-02-22

Family

ID=25434229

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7914858A Withdrawn FR2432170A1 (fr) 1978-06-12 1979-06-11 Procede de mesure de recepteurs d'antigene sur des membranes de cellules

Country Status (4)

Country Link
JP (1) JPS551594A (fr)
DE (1) DE2923735A1 (fr)
FR (1) FR2432170A1 (fr)
GB (1) GB2022826A (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341055C (fr) * 1987-12-08 2000-07-18 Alan Mcclelland Lignees cellulaires transfectantes exprimant le recepteur majeur du rhinovirus chez l'homme
US6514936B1 (en) 1988-09-01 2003-02-04 Bayer Corporation Antiviral methods using human rhinovirus receptor (ICAM-1)
US6051231A (en) 1988-09-01 2000-04-18 Bayer Corporation Antiviral methods and prepations
ES2141076T3 (es) 1988-09-01 2000-03-16 Bayer Ag Proteina del receptor de rinovirus humano que inhibe la infectividad del virus.
ZA896668B (en) * 1988-09-01 1990-06-27 Molecular Therapeutics Inc A human rhinovirus receptor protein that inhibits virus infectivity
US6143298A (en) * 1988-09-01 2000-11-07 Bayer Corporation Soluble truncated forms of ICAM-1
US5686582A (en) * 1990-07-20 1997-11-11 Bayer Corporation Multimeric forms of human rhinovirus receptor protein
US5686581A (en) * 1990-07-20 1997-11-11 Bayer Corporation Multimeric form of human rhinovirus receptor protein
US6107461A (en) * 1990-07-20 2000-08-22 Bayer Corporation Multimeric forms of human rhinovirus receptor and fragments thereof, and method of use
DE69131564T2 (de) * 1990-07-20 2000-01-13 Bayer Ag Multimere Formen des menschlichen Rhinovirus-Rezeptorproteins

Also Published As

Publication number Publication date
DE2923735A1 (de) 1979-12-13
JPS551594A (en) 1980-01-08
GB2022826A (en) 1979-12-19

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Legal Events

Date Code Title Description
ST Notification of lapse