FR2432170A1 - Procede de mesure de recepteurs d'antigene sur des membranes de cellules - Google Patents
Procede de mesure de recepteurs d'antigene sur des membranes de cellulesInfo
- Publication number
- FR2432170A1 FR2432170A1 FR7914858A FR7914858A FR2432170A1 FR 2432170 A1 FR2432170 A1 FR 2432170A1 FR 7914858 A FR7914858 A FR 7914858A FR 7914858 A FR7914858 A FR 7914858A FR 2432170 A1 FR2432170 A1 FR 2432170A1
- Authority
- FR
- France
- Prior art keywords
- cell membranes
- antigen receptors
- measuring antigen
- degrees
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000006306 Antigen Receptors Human genes 0.000 title abstract 2
- 108010083359 Antigen Receptors Proteins 0.000 title abstract 2
- 210000000170 cell membrane Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 3
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention se rapporte à l'analyse biologique. Elle concerne un procédé pour mesurer des récepteurs d'antigène sur des membranes de cellules, caractérisé en ce que les cellules sont mélangées avec une solution aqueuse isotonique d'un antigène immunoréactif soluble dans l'eau, marqué qui est d'une pureté d'au moins 95 % et est immunoréactif à au moins 40 %, le mélange est mis à incuber à une température comprise entre 4 degrés C et 40 degrés C pendant un temps suffisant pour qu'on arrive à la liaison à l'équilibre, on récolte les cellules à partir du mélange ayant subi l'incubation, et on mesure la quantité d'antigène marqué fixée sur les cellules récoltées. Utilisation par les laboratoires d'analyse.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91434778A | 1978-06-12 | 1978-06-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
FR2432170A1 true FR2432170A1 (fr) | 1980-02-22 |
Family
ID=25434229
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7914858A Withdrawn FR2432170A1 (fr) | 1978-06-12 | 1979-06-11 | Procede de mesure de recepteurs d'antigene sur des membranes de cellules |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPS551594A (fr) |
DE (1) | DE2923735A1 (fr) |
FR (1) | FR2432170A1 (fr) |
GB (1) | GB2022826A (fr) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1341055C (fr) * | 1987-12-08 | 2000-07-18 | Alan Mcclelland | Lignees cellulaires transfectantes exprimant le recepteur majeur du rhinovirus chez l'homme |
US6514936B1 (en) | 1988-09-01 | 2003-02-04 | Bayer Corporation | Antiviral methods using human rhinovirus receptor (ICAM-1) |
US6143298A (en) * | 1988-09-01 | 2000-11-07 | Bayer Corporation | Soluble truncated forms of ICAM-1 |
DE68929096T2 (de) | 1988-09-01 | 2000-05-11 | Bayer Corp., Pittsburgh | Menschliches Rhinovirusrezeptorprotein, das die Virusinfektionsanfälligkeit hemmt |
ZA896668B (en) * | 1988-09-01 | 1990-06-27 | Molecular Therapeutics Inc | A human rhinovirus receptor protein that inhibits virus infectivity |
US6051231A (en) | 1988-09-01 | 2000-04-18 | Bayer Corporation | Antiviral methods and prepations |
US6107461A (en) * | 1990-07-20 | 2000-08-22 | Bayer Corporation | Multimeric forms of human rhinovirus receptor and fragments thereof, and method of use |
US5871733A (en) * | 1990-07-20 | 1999-02-16 | Bayer Corporation | Multimeric forms of human rhinovirus receptor protein |
US5686581A (en) * | 1990-07-20 | 1997-11-11 | Bayer Corporation | Multimeric form of human rhinovirus receptor protein |
DE69131564T2 (de) * | 1990-07-20 | 2000-01-13 | Bayer Corp., Pittsburgh | Multimere Formen des menschlichen Rhinovirus-Rezeptorproteins |
-
1979
- 1979-06-11 GB GB7920277A patent/GB2022826A/en not_active Withdrawn
- 1979-06-11 FR FR7914858A patent/FR2432170A1/fr not_active Withdrawn
- 1979-06-11 JP JP7346279A patent/JPS551594A/ja active Pending
- 1979-06-12 DE DE19792923735 patent/DE2923735A1/de not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
GB2022826A (en) | 1979-12-19 |
JPS551594A (en) | 1980-01-08 |
DE2923735A1 (de) | 1979-12-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FR2432170A1 (fr) | Procede de mesure de recepteurs d'antigene sur des membranes de cellules | |
Schroeder et al. | [37] Monitoring specific protein-binding reactions with chemiluminescence | |
Shewring et al. | An improved radioreceptor assay for TSH receptor antibodies | |
Tabor et al. | Intermediate steps in the formylation of tetrahydrofolic acid by formiminoglutamic acid in rabbit liver | |
Hammarström et al. | Pokeweed mitogen induced differentiation of human B cells: evaluation by a protein A haemolytic plaque assay. | |
DE3169720D1 (en) | Method for analytical assays and rotor element for carrying out the method | |
IE790802L (en) | Cytological assay | |
FR2415301A1 (fr) | Procede et composition pour determination par fixation specifique avec un recepteur double d'un ligand echantillon | |
FR2401423A1 (fr) | Dosage selectif de cellules somatiques et microbiennes viables | |
FR2443103A1 (fr) | Procede d'echantillonnage d'objet | |
IE812927L (en) | Autonomous simulataneous analysis apparatus | |
FR2426262A1 (fr) | Procede d'essai de substances biologiques en phase solide | |
Donovan | Spectrophotometric observation of the alkaline hydrolysis of protein disulfide bonds | |
NO873258L (no) | Fremgangsmaate og analysesett til bestemmelse av frie virksomme stoffer i biologiske vaesker. | |
Wilder et al. | Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays | |
US5030554A (en) | Conservative whole blood sample preparation technique | |
FR2365800A1 (fr) | Procede de determination d'un reactif d'une reaction immunologique | |
FR2378281A1 (fr) | Reactif et procede d'analyse de la creatinine dans les liquides biologiques | |
Tew et al. | A radioimmunoassay for human antibody specific for microbial antigens | |
EP0166583A3 (en) | Compositions for enzyme immunoassay of prostaglandins | |
KR890700831A (ko) | 생체액중의 알부민 측정법 | |
Wang et al. | Enhanced luminescence enzyme immunoassay for factor VIII related antigen. | |
Götze et al. | A rapid micromethod for direct H-2 typing of mouse cultured lymphoid cells | |
SU1264933A1 (ru) | Способ оценки воспроизводительных способностей свиноматок | |
US4041147A (en) | Angiotensin iradioimmunoassay |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |