FR2388585A1 - Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion - Google Patents

Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion

Info

Publication number
FR2388585A1
FR2388585A1 FR7712518A FR7712518A FR2388585A1 FR 2388585 A1 FR2388585 A1 FR 2388585A1 FR 7712518 A FR7712518 A FR 7712518A FR 7712518 A FR7712518 A FR 7712518A FR 2388585 A1 FR2388585 A1 FR 2388585A1
Authority
FR
France
Prior art keywords
protein
esp
sepn
passivated
silica gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7712518A
Other languages
English (en)
Other versions
FR2388585B1 (fr
Inventor
Charles Dulout
Andre Peyrouset
Rene Panaris
Claude Hannoun
Jean Vincent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe National Elf Aquitaine
Societe Nationale Elf Aquitaine Production SA
Original Assignee
Societe National Elf Aquitaine
Societe Nationale Elf Aquitaine Production SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe National Elf Aquitaine, Societe Nationale Elf Aquitaine Production SA filed Critical Societe National Elf Aquitaine
Priority to FR7712518A priority Critical patent/FR2388585A1/fr
Priority to FR7810769A priority patent/FR2422720A2/fr
Priority to DE19782817871 priority patent/DE2817871A1/de
Priority to CA301,897A priority patent/CA1111415A/fr
Priority to JP4916878A priority patent/JPS53140097A/ja
Priority to GB16321/78A priority patent/GB1595378A/en
Priority to ES469156A priority patent/ES469156A1/es
Priority to IT2269278A priority patent/IT1095614B/it
Priority to SU782614452A priority patent/SU784784A3/ru
Priority to US05/900,081 priority patent/US4199450A/en
Publication of FR2388585A1 publication Critical patent/FR2388585A1/fr
Application granted granted Critical
Publication of FR2388585B1 publication Critical patent/FR2388585B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28057—Surface area, e.g. B.E.T specific surface area
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/327—Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procédé pour la séparation et la purification de protéines, et/ou d'unités morphologiquement organisées (virus), par chromatographie d'exclusion, en phase aqueuse, sur un support rigide de silice présentant des caractéristiques particulières, opération combinee ou non avec des procédés connus en soi de separation, utilisant des principes différents de celui de l'encombrement spatial. Ce procédé s'applique en particulier à la séparation ou/et purification de virus à partir du milieu de culture de ces derniers. Ce procédé concerne tout particulièrement la séparation et purification des virus de la grippe, obtenus avec des rendements voisins de 100 %, et parfaitement purs.
FR7712518A 1977-04-26 1977-04-26 Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion Granted FR2388585A1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR7712518A FR2388585A1 (fr) 1977-04-26 1977-04-26 Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion
FR7810769A FR2422720A2 (fr) 1977-04-26 1978-04-12 Separation et purification de proteines par chromatographie
DE19782817871 DE2817871A1 (de) 1977-04-26 1978-04-24 Verfahren und vorrichtung zur abtrennung und reinigung von proteinen durch chromatographie
JP4916878A JPS53140097A (en) 1977-04-26 1978-04-25 Separation and purification of protein by chromatography
CA301,897A CA1111415A (fr) 1977-04-26 1978-04-25 Separation et purification de proteines par chromatographie
GB16321/78A GB1595378A (en) 1977-04-26 1978-04-25 Chromatographic separation and purification of proteins and viruses
ES469156A ES469156A1 (es) 1977-04-26 1978-04-26 Procedimiento y dispositivo de separacion y purificacion de proteinas por cromatografia
IT2269278A IT1095614B (it) 1977-04-26 1978-04-26 Procedimento per la separazione e purificazione di proteine per cromatografia e dispositivo per la sua attuazione
SU782614452A SU784784A3 (ru) 1977-04-26 1978-04-26 Способ выделени протеина
US05/900,081 US4199450A (en) 1977-04-26 1978-04-26 Process of separating from an aqueous medium a protein or a morphologically organized unit by liquid exclusion chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7712518A FR2388585A1 (fr) 1977-04-26 1977-04-26 Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion

Publications (2)

Publication Number Publication Date
FR2388585A1 true FR2388585A1 (fr) 1978-11-24
FR2388585B1 FR2388585B1 (fr) 1981-10-16

Family

ID=9189909

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7712518A Granted FR2388585A1 (fr) 1977-04-26 1977-04-26 Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion

Country Status (1)

Country Link
FR (1) FR2388585A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2533456A1 (fr) * 1982-09-28 1984-03-30 Pharmuka Lab Installation automatique pour chromatographie liquide
US4478713A (en) * 1983-09-23 1984-10-23 Pharmuka Laboratoires Automatic installation for liquid chromatography
WO2005070513A1 (fr) * 2004-01-09 2005-08-04 Alltech Associates Inc. Recuperation amelioree d'analytes en chromatographie industrielle

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2533456A1 (fr) * 1982-09-28 1984-03-30 Pharmuka Lab Installation automatique pour chromatographie liquide
EP0107544A2 (fr) * 1982-09-28 1984-05-02 Pharmuka Laboratoires Installation automatique pour chromatographie liquide
EP0107544A3 (en) * 1982-09-28 1984-06-20 Pharmuka Laboratoires Automatic system for liquid chromatography
US4478713A (en) * 1983-09-23 1984-10-23 Pharmuka Laboratoires Automatic installation for liquid chromatography
WO2005070513A1 (fr) * 2004-01-09 2005-08-04 Alltech Associates Inc. Recuperation amelioree d'analytes en chromatographie industrielle

Also Published As

Publication number Publication date
FR2388585B1 (fr) 1981-10-16

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